pycom14g04090

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0019801
Physical Location & Seq
Forward (+)
3510406 .. 3511672
1267 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04090.1

Sequence Viewer

Length: 489 bp
ATGGATCTTAGCTGTCTCTGTAATTTGTACTCTCTTTCCTATATTCACCATGTCTGCAACTACAAGCTCAGACTGCGAGAATCCCGACTCCTTACTACAAGTTCAGACTGCAAGGATCCCGGCTCCTTAGCCATGTCTGCAACTAAAAGCTCTCCGCCAGCGTGCGGCGCAATCTGTTTTGGCGTCTTTGTGATTGCTCTCTTTCTCGTGCCTTTTGCTGTTTTGATTGGGGTCCAGTTTCAGGCCAGCAAAAGAAATTCGGAGTACAAATGCGCCGACTTTACCATCAACGGTGCTTATCTCACAGTTAGCTCGCTCATTTATTCGGTTGAGATTATAAACATTGTTCTACTATCTTTCAAAAGAGTTAACTATATCAACGAAAATAATCGAATTCAATTCAACTTTAATTACTTTACATGTACTCGTTCCCGGGTTCTCTTTGCATTCCATCATCAAACTCTCAAATATCCCGCCAAAATCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

163

Amino Acids

18.35

Weight (kDa)

9.17

Isoelectric Point (pI)

46.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019801)

Species Orthologous Gene IDs
prunus_persica Prupe.1G185400_v2.0.a1 Prupe.1G413200_v2.0.a1 Prupe.6G103200_v2.0.a1
pyrus_communis pycom05g01680 pycom14g04070 pycom14g04080 pycom14g04090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 338
AciI CCGC 3 cut(s) 155, 165, 474
AclWI GGATC 3 cut(s) 12, 110, 123
AcsI RAATTY 2 cut(s) 256, 393
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 3 cut(s) 29, 266, 424
AfiI CCNNNNNNNGG 2 cut(s) 164, 241
AflIII ACRYGT 1 cut(s) 419
AgsI TTSAA 3 cut(s) 361, 398, 403
AluBI AGCT 4 cut(s) 12, 67, 150, 312
AluI AGCT 4 cut(s) 12, 67, 150, 312
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 3 cut(s) 12, 110, 123
Ama87I CYCGRG 1 cut(s) 432
AoxI GGCC 1 cut(s) 243
ApoI RAATTY 2 cut(s) 256, 393
AspLEI GCGC 2 cut(s) 170, 275
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 3 cut(s) 120, 433, 434
AsuHPI GGTGA 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 432
AvaII GGWCC 1 cut(s) 232
BamHI GGATCC 1 cut(s) 115
BauI CACGAG 1 cut(s) 206
BccI CCATC 2 cut(s) 293, 459
BcnI CCSGG 3 cut(s) 120, 433, 434
BcoDI GTCTC 1 cut(s) 20
BisI GCNGC 1 cut(s) 166
BlsI GCNGC 1 cut(s) 167
Bme1390I CCNGG 3 cut(s) 120, 433, 434
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 432
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 3 cut(s) 117, 124, 233
BmrFI CCNGG 3 cut(s) 120, 433, 434
Bpu10I CCTNAGC 1 cut(s) 127
BpuMI CCSGG 3 cut(s) 120, 433, 434
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 432
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 241
Bse1I ACTGG 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 432
BseLI CCNNNNNNNGG 2 cut(s) 164, 241
BseMII CTCAG 1 cut(s) 82
BseNI ACTGG 1 cut(s) 235
BshFI GGCC 1 cut(s) 245
BsiHKCI CYCGRG 1 cut(s) 432
BsiSI CCGG 2 cut(s) 120, 433
BslI CCNNNNNNNGG 2 cut(s) 164, 241
BsmAI GTCTC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 432
Bsp143I GATC 2 cut(s) 4, 115
BspACI CCGC 3 cut(s) 155, 165, 474
BspANI GGCC 1 cut(s) 245
BspCNI CTCAG 1 cut(s) 81
BspLI GGNNCC 3 cut(s) 117, 124, 233
BspPI GGATC 3 cut(s) 12, 110, 123
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 1 cut(s) 432
BssMI GATC 2 cut(s) 4, 115
BssNI GRCGYC 1 cut(s) 183
BssSI CACGAG 1 cut(s) 206
Bst2BI CACGAG 1 cut(s) 206
Bst4CI ACNGT 2 cut(s) 293, 307
BstACI GRCGYC 1 cut(s) 183
BstC8I GCNNGC 4 cut(s) 159, 163, 247, 314
BstDEI CTNAG 3 cut(s) 8, 68, 127
BstHHI GCGC 2 cut(s) 170, 275
BstKTI GATC 2 cut(s) 7, 118
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 2 cut(s) 4, 115
BstMWI GCNNNNNNNGC 3 cut(s) 73, 137, 167
BstNSI RCATGY 1 cut(s) 423
BstSCI CCNGG 3 cut(s) 118, 431, 432
BstX2I RGATCY 2 cut(s) 4, 115
BstYI RGATCY 2 cut(s) 4, 115
BsuRI GGCC 1 cut(s) 245
Cac8I GCNNGC 4 cut(s) 159, 163, 247, 314
CfoI GCGC 2 cut(s) 170, 275
Cfr13I GGNCC 1 cut(s) 232
Cfr9I CCCGGG 1 cut(s) 432
CseI GACGC 1 cut(s) 172
Csp6I GTAC 3 cut(s) 28, 265, 423
CviAII CATG 3 cut(s) 50, 133, 420
CviJI RGCY 7 cut(s) 12, 67, 123, 131, 150, 245, 312
CviKI_1 RGCY 7 cut(s) 12, 67, 123, 131, 150, 245, 312
CviQI GTAC 3 cut(s) 28, 265, 423
DdeI CTNAG 3 cut(s) 8, 68, 127
DpnI GATC 2 cut(s) 6, 117
DpnII GATC 2 cut(s) 4, 115
EciI GGCGGA 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 232
Eco88I CYCGRG 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 393
FaeI CATG 3 cut(s) 53, 136, 423
FaiI YATR 6 cut(s) 42, 51, 134, 338, 375, 421
FatI CATG 3 cut(s) 49, 132, 419
FauI CCCGC 1 cut(s) 481
Fnu4HI GCNGC 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 166
GlaI GCGC 2 cut(s) 169, 274
GluI GCNGC 1 cut(s) 166
HaeIII GGCC 1 cut(s) 245
HapII CCGG 2 cut(s) 120, 433
HgaI GACGC 1 cut(s) 172
HhaI GCGC 2 cut(s) 170, 275
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 3 cut(s) 53, 136, 423
Hin6I GCGC 2 cut(s) 168, 273
HinP1I GCGC 2 cut(s) 168, 273
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 2 cut(s) 80, 87
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 2 cut(s) 120, 433
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 3 cut(s) 71, 106, 262
Hpy188III TCNNGA 1 cut(s) 84
Hpy8I GTNNAC 1 cut(s) 370
HpyCH4III ACNGT 2 cut(s) 293, 307
HpyCH4V TGCA 4 cut(s) 57, 111, 140, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 137, 167
HpyF3I CTNAG 3 cut(s) 8, 68, 127
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 3 cut(s) 53, 136, 423
HspAI GCGC 2 cut(s) 168, 273
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 2 cut(s) 4, 115
LmnI GCTCC 1 cut(s) 128
LpnPI CCDG 6 cut(s) 133, 171, 227, 248, 259, 446
MalI GATC 2 cut(s) 6, 117
MboI GATC 2 cut(s) 4, 115
MflI RGATCY 2 cut(s) 4, 115
MluCI AATT 5 cut(s) 22, 256, 393, 398, 409
MlyI GAGTC 1 cut(s) 81
MseI TTAA 2 cut(s) 369, 408
MspI CCGG 2 cut(s) 120, 433
MspR9I CCNGG 3 cut(s) 120, 433, 434
Mva1269I GAATGC 1 cut(s) 446
MwoI GCNNNNNNNGC 3 cut(s) 73, 137, 167
NciI CCSGG 3 cut(s) 120, 433, 434
NdeII GATC 2 cut(s) 4, 115
NlaIII CATG 3 cut(s) 53, 136, 423
NlaIV GGNNCC 3 cut(s) 117, 124, 233
NspI RCATGY 1 cut(s) 423
PciI ACATGT 1 cut(s) 419
PctI GAATGC 1 cut(s) 446
PfeI GAWTC 1 cut(s) 80
PkrI GCNGC 1 cut(s) 167
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
PscI ACATGT 1 cut(s) 419
PsiI TTATAA 1 cut(s) 338
PspN4I GGNNCC 3 cut(s) 117, 124, 233
PspPI GGNCC 1 cut(s) 232
PsuI RGATCY 2 cut(s) 4, 115
RsaI GTAC 3 cut(s) 29, 266, 424
RsaNI GTAC 3 cut(s) 28, 265, 423
SaqAI TTAA 2 cut(s) 369, 408
SatI GCNGC 1 cut(s) 166
Sau3AI GATC 2 cut(s) 4, 115
Sau96I GGNCC 1 cut(s) 232
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 3 cut(s) 120, 433, 434
SetI ASST 4 cut(s) 14, 69, 152, 314
SinI GGWCC 1 cut(s) 232
SmaI CCCGGG 1 cut(s) 434
Sse9I AATT 5 cut(s) 22, 256, 393, 398, 409
SsiI CCGC 3 cut(s) 155, 165, 474
StyD4I CCNGG 3 cut(s) 118, 431, 432
TaaI ACNGT 2 cut(s) 293, 307
TaqI TCGA 1 cut(s) 391
TasI AATT 5 cut(s) 22, 256, 393, 398, 409
TatI WGTACW 3 cut(s) 27, 264, 422
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 2 cut(s) 369, 408
Tru9I TTAA 2 cut(s) 369, 408
TspMI CCCGGG 1 cut(s) 432
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 2 cut(s) 256, 393
XceI RCATGY 1 cut(s) 423
XmaI CCCGGG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.