pycom14g04070

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0019801
Physical Location & Seq
Forward (+)
3507325 .. 3508047
723 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04070.2

Sequence Viewer

Length: 672 bp
ATGAGAGAGACTCACTCTTGTTATACTACGTGGAATAGTTATATATTAGTCTACAACTTAACCCCGTTTTTCACCCATTGTAACTCTGCAACTACAACCTCAGACTGTGAGAATCCCCGCTTCTTAGCCATGTCTGCAACCGAAAGATCTCCGCCAGCGTGCAACAAAATCTGTGCCGCCAGCACTATGATTATGGTTTTTGCTGCTATGATTGGGCTTGTGTTGTGGTTCATCATACAAACTACTTCGGGTTATACCGAAAGCAATCTTACTCTCTCGTACAACCTCGCCCTCAACATCACCCTCACAAACCCTAAGAAGAAACTTTTCGACGCGTTGATTGACGTCAAAGTCAGTGCATACTATCAAGACAAGAGAATTGGTCAGGTGACTTTGGTGGATCGGTCGATGTCGACATCCAAGAAGACAACCGTTTTTCAGAATGTAGTGGTTCGACCTCGCGGCATGTTGTTTGAGGAATTTGTGACCTCTGCAGCTGATCTTTACAGATCGACGTGTCCATTACTTTCCAGGACAAGAACAATGTTCCTCGGCGAGGGGTCATCTCCAGCCTACAGGGATGAGGTCATATACAATCTATGGCTTCCTTTGAGTTTCAACGGGACATCTTGGGATGCTGACAAGACTACACACTGCCCCATAGATACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

224

Amino Acids

25.14

Weight (kDa)

8.13

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019801)

Species Orthologous Gene IDs
prunus_persica Prupe.1G185400_v2.0.a1 Prupe.1G413200_v2.0.a1 Prupe.6G103200_v2.0.a1
pyrus_communis pycom05g01680 pycom14g04070 pycom14g04080 pycom14g04090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
AatII GACGTC 1 cut(s) 348
AccI GTMKAC 2 cut(s) 51, 413
AccII CGCG 2 cut(s) 335, 462
AciI CCGC 4 cut(s) 118, 152, 177, 462
AclWI GGATC 1 cut(s) 408
AcsI RAATTY 1 cut(s) 479
AcyI GRCGYC 1 cut(s) 345
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 556
AflIII ACRYGT 2 cut(s) 333, 515
AgsI TTSAA 1 cut(s) 619
AjiI CACGTC 1 cut(s) 516
AjnI CCWGG 1 cut(s) 530
AluBI AGCT 1 cut(s) 497
AluI AGCT 1 cut(s) 497
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 1 cut(s) 408
ApeKI GCWGC 2 cut(s) 203, 494
ApoI RAATTY 1 cut(s) 479
Asp700I GAANNNNTTC 1 cut(s) 326
AsuHPI GGTGA 3 cut(s) 64, 292, 400
BbsI GAAGAC 1 cut(s) 431
BbvI GCAGC 2 cut(s) 190, 506
BciT130I CCWGG 1 cut(s) 532
BcoDI GTCTC 1 cut(s) 2
BfmI CTRYAG 2 cut(s) 492, 574
BglII AGATCT 1 cut(s) 146
BisI GCNGC 4 cut(s) 177, 204, 463, 495
BlsI GCNGC 4 cut(s) 178, 205, 464, 496
Bme1390I CCNGG 1 cut(s) 532
BmgBI CACGTC 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 532
BmsI GCATC 1 cut(s) 625
BpiI GAAGAC 1 cut(s) 431
BplI GAGNNNNNCTC 2 cut(s) 27, 31
BpmI CTGGAG 1 cut(s) 552
BsaAI YACGTR 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 550
Bsc4I CCNNNNNNNGG 1 cut(s) 556
BseBI CCWGG 1 cut(s) 532
BseDI CCNNGG 1 cut(s) 550
BseGI GGATG 3 cut(s) 416, 586, 640
BseLI CCNNNNNNNGG 1 cut(s) 556
BseMII CTCAG 1 cut(s) 114
BseXI GCAGC 2 cut(s) 190, 506
Bsh1236I CGCG 2 cut(s) 335, 462
Bsh1285I CGRYCG 1 cut(s) 407
BsiEI CGRYCG 1 cut(s) 407
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 1 cut(s) 556
BsmAI GTCTC 1 cut(s) 2
BsmFI GGGAC 1 cut(s) 637
Bsp143I GATC 4 cut(s) 146, 400, 499, 509
BspACI CCGC 4 cut(s) 118, 152, 177, 462
BspCNI CTCAG 1 cut(s) 113
BspFNI CGCG 2 cut(s) 335, 462
BspMAI CTGCAG 1 cut(s) 496
BspPI GGATC 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 550
BssMI GATC 4 cut(s) 146, 400, 499, 509
BssNI GRCGYC 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 532
Bst4CI ACNGT 2 cut(s) 107, 433
BstACI GRCGYC 1 cut(s) 345
BstBAI YACGTR 1 cut(s) 30
BstC8I GCNNGC 3 cut(s) 156, 160, 181
BstDEI CTNAG 4 cut(s) 100, 124, 315, 669
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 3 cut(s) 416, 586, 640
BstFNI CGCG 2 cut(s) 335, 462
BstKTI GATC 4 cut(s) 149, 403, 502, 512
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 4 cut(s) 146, 400, 499, 509
BstMCI CGRYCG 1 cut(s) 407
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 532
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 530
BstSFI CTRYAG 2 cut(s) 492, 574
BstUI CGCG 2 cut(s) 335, 462
BstV1I GCAGC 2 cut(s) 190, 506
BstV2I GAAGAC 1 cut(s) 431
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BtrI CACGTC 1 cut(s) 516
BtsCI GGATG 3 cut(s) 416, 586, 640
BtsI GCAGTG 1 cut(s) 652
BtsIMutI CAGTG 2 cut(s) 361, 652
Cac8I GCNNGC 3 cut(s) 156, 160, 181
CseI GACGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 280
CviAII CATG 2 cut(s) 130, 466
CviJI RGCY 5 cut(s) 128, 217, 497, 572, 604
CviKI_1 RGCY 5 cut(s) 128, 217, 497, 572, 604
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 4 cut(s) 100, 124, 315, 669
DpnI GATC 4 cut(s) 148, 402, 501, 511
DpnII GATC 4 cut(s) 146, 400, 499, 509
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
EciI GGCGGA 1 cut(s) 141
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 530
FaeI CATG 2 cut(s) 133, 469
FaqI GGGAC 1 cut(s) 637
FatI CATG 2 cut(s) 129, 465
FauI CCCGC 1 cut(s) 125
FblI GTMKAC 2 cut(s) 51, 413
Fnu4HI GCNGC 4 cut(s) 177, 204, 463, 495
FokI GGATG 3 cut(s) 403, 593, 647
Fsp4HI GCNGC 4 cut(s) 177, 204, 463, 495
GluI GCNGC 4 cut(s) 177, 204, 463, 495
GsuI CTGGAG 1 cut(s) 552
HgaI GACGC 1 cut(s) 341
Hin1I GRCGYC 1 cut(s) 345
Hin1II CATG 2 cut(s) 133, 469
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 2 cut(s) 10, 112
HphI GGTGA 3 cut(s) 64, 292, 400
Hpy166II GTNNAC 2 cut(s) 52, 414
Hpy188I TCNGA 2 cut(s) 103, 441
Hpy188III TCNNGA 1 cut(s) 368
Hpy8I GTNNAC 2 cut(s) 52, 414
Hpy99I CGWCG 2 cut(s) 335, 517
HpyCH4III ACNGT 2 cut(s) 107, 433
HpyCH4IV ACGT 3 cut(s) 29, 345, 515
HpyCH4V TGCA 5 cut(s) 89, 137, 162, 359, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 4 cut(s) 100, 124, 315, 669
HpySE526I ACGT 3 cut(s) 29, 345, 515
Hsp92I GRCGYC 1 cut(s) 345
Hsp92II CATG 2 cut(s) 133, 469
Kzo9I GATC 4 cut(s) 146, 400, 499, 509
LpnPI CCDG 7 cut(s) 168, 193, 371, 517, 544, 562, 582
Lsp1109I GCAGC 2 cut(s) 190, 506
LweI GCATC 1 cut(s) 625
MaeII ACGT 3 cut(s) 29, 345, 515
MaeIII GTNAC 3 cut(s) 80, 388, 484
MalI GATC 4 cut(s) 148, 402, 501, 511
MboI GATC 4 cut(s) 146, 400, 499, 509
MboII GAAGA 2 cut(s) 331, 436
MflI RGATCY 1 cut(s) 146
MluCI AATT 2 cut(s) 378, 479
MluI ACGCGT 1 cut(s) 333
MlyI GAGTC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 326
MseI TTAA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 497
MspR9I CCNGG 1 cut(s) 532
MvaI CCWGG 1 cut(s) 532
MvnI CGCG 2 cut(s) 335, 462
MwoI GCNNNNNNNGC 1 cut(s) 134
NdeII GATC 4 cut(s) 146, 400, 499, 509
NlaIII CATG 2 cut(s) 133, 469
NmeAIII GCCGAG 1 cut(s) 531
NmuCI GTSAC 2 cut(s) 388, 484
NspI RCATGY 1 cut(s) 469
PdmI GAANNNNTTC 1 cut(s) 326
PfeI GAWTC 1 cut(s) 112
PfoI TCCNGGA 1 cut(s) 530
PkrI GCNGC 4 cut(s) 178, 205, 464, 496
PleI GAGTC 1 cut(s) 4
PpsI GAGTC 1 cut(s) 4
Ppu21I YACGTR 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 530
PspGI CCWGG 1 cut(s) 530
PstI CTGCAG 1 cut(s) 496
PsuI RGATCY 1 cut(s) 146
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SalI GTCGAC 1 cut(s) 412
SaqAI TTAA 1 cut(s) 59
SatI GCNGC 4 cut(s) 177, 204, 463, 495
Sau3AI GATC 4 cut(s) 146, 400, 499, 509
SchI GAGTC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 532
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 2 cut(s) 492, 574
Sse9I AATT 2 cut(s) 378, 479
SsiI CCGC 4 cut(s) 118, 152, 177, 462
StyD4I CCNGG 1 cut(s) 530
TaaI ACNGT 2 cut(s) 107, 433
TaiI ACGT 3 cut(s) 32, 348, 518
TaqI TCGA 5 cut(s) 330, 407, 413, 454, 512
TaqII GACCGA 1 cut(s) 393
TasI AATT 2 cut(s) 378, 479
TauI GCSGC 2 cut(s) 179, 465
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TscAI CASTG 2 cut(s) 361, 659
TseFI GTSAC 2 cut(s) 388, 484
TseI GCWGC 2 cut(s) 203, 494
Tsp45I GTSAC 2 cut(s) 388, 484
TspDTI ATGAA 1 cut(s) 220
TspRI CASTG 2 cut(s) 361, 659
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 1 cut(s) 479
XceI RCATGY 1 cut(s) 469
XmiI GTMKAC 2 cut(s) 51, 413
XmnI GAANNNNTTC 1 cut(s) 326
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.