pycom14g04080

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0019801
Physical Location & Seq
Forward (+)
3508762 .. 3509454
693 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g04080.1

Sequence Viewer

Length: 693 bp
ATGTCTGCAACTACAACCTCAGACTGCGAGAATCCCCGCTCCTTAGCCATGTCTGCAACTGAAAGATCTCCGCCAGCGTGCAACAAAATCTGTGCCGCCCGCACTATGATTATGGTTTTTGCTGCTATGATTGGGCTTGTGTTGTGGTTCACCATAAAAAGTATTTCGAAGTACAAATGCCCCGACTTCACCATCAACGGTGCTTATCTCAGCCAATTCAGTTATACCGAAAGCAATCTTACTCTCTCGTACAACCTCGCCCTCAACATCACCCTCACAAACCCTAAGAAGAAAATCTTCCACGCGTTGATTGACGTCGAAGTCAGTGCATACTATCAAGACAAGAGACTTGGTCAGGTGACTTTGGTGGATCGATCGATGTCGACATCCAAGAAGACAACCGTTTTTCAGAATGTAGTGGTTCGAGGGCACGACATGTTGTTTGAGGAATTTGTGACCCCTGCTGCTGATCTTTACAGTATCGACGTGGTCATTACTTTCCAGGACAAGAACTATACAAAGTCAACAGATCTTACTGAATCTAAAATATTATGGCTACCCAAACCGTACAATGTTCATTGGCGAGGGGACATCCACAGCGGTGAGGTCATCTACAATCTAAGGCTTCCTTTGAGTTTCAACGGGACATCTTGGGATGCTGACAAGACTACACACTGCCCCATAGATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

231

Amino Acids

25.96

Weight (kDa)

7.63

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019801)

Species Orthologous Gene IDs
prunus_persica Prupe.1G185400_v2.0.a1 Prupe.1G413200_v2.0.a1 Prupe.6G103200_v2.0.a1
pyrus_communis pycom05g01680 pycom14g04070 pycom14g04080 pycom14g04090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 320
AatII GACGTC 1 cut(s) 318
AccBSI CCGCTC 1 cut(s) 39
AccI GTMKAC 1 cut(s) 383
AccII CGCG 1 cut(s) 305
AciI CCGC 5 cut(s) 37, 71, 96, 100, 600
AclWI GGATC 1 cut(s) 378
AcsI RAATTY 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 3 cut(s) 173, 251, 569
AflIII ACRYGT 2 cut(s) 303, 435
AgsI TTSAA 1 cut(s) 640
AjiI CACGTC 1 cut(s) 487
AjnI CCWGG 1 cut(s) 501
AleI CACNNNNGTG 1 cut(s) 600
Alw26I GTCTC 1 cut(s) 340
AlwI GGATC 1 cut(s) 378
ApeKI GCWGC 2 cut(s) 122, 464
ApoI RAATTY 1 cut(s) 449
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
Asp700I GAANNNNTTC 1 cut(s) 296
AsuHPI GGTGA 5 cut(s) 142, 181, 262, 370, 614
AsuII TTCGAA 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 432
BbsI GAAGAC 1 cut(s) 401
BbvI GCAGC 2 cut(s) 109, 451
BccI CCATC 1 cut(s) 200
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 1 cut(s) 503
BcoDI GTCTC 1 cut(s) 340
BglII AGATCT 2 cut(s) 65, 529
BisI GCNGC 3 cut(s) 96, 123, 465
BlsI GCNGC 3 cut(s) 97, 124, 466
Bme1390I CCNGG 1 cut(s) 503
BmgBI CACGTC 1 cut(s) 487
BmrFI CCNGG 1 cut(s) 503
BmsI GCATC 1 cut(s) 646
BpiI GAAGAC 1 cut(s) 401
Bpu10I CCTNAGC 1 cut(s) 43
Bpu14I TTCGAA 1 cut(s) 167
Bsa29I ATCGAT 2 cut(s) 373, 377
BsaHI GRCGYC 1 cut(s) 315
BseBI CCWGG 1 cut(s) 503
BseCI ATCGAT 2 cut(s) 373, 377
BseGI GGATG 3 cut(s) 386, 591, 661
BseMII CTCAG 2 cut(s) 33, 223
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 2 cut(s) 109, 451
Bsh1236I CGCG 1 cut(s) 305
Bsh1285I CGRYCG 1 cut(s) 377
BshVI ATCGAT 2 cut(s) 373, 377
BsiEI CGRYCG 1 cut(s) 377
BslFI GGGAC 2 cut(s) 602, 658
BsmAI GTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 602, 658
Bsp119I TTCGAA 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 5 cut(s) 65, 370, 374, 469, 529
BspACI CCGC 5 cut(s) 37, 71, 96, 100, 600
BspCNI CTCAG 2 cut(s) 32, 222
BspDI ATCGAT 2 cut(s) 373, 377
BspFNI CGCG 1 cut(s) 305
BspPI GGATC 1 cut(s) 378
BspT104I TTCGAA 1 cut(s) 167
BsrBI CCGCTC 1 cut(s) 39
BssMI GATC 5 cut(s) 65, 370, 374, 469, 529
BssNI GRCGYC 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 4 cut(s) 200, 403, 479, 567
BstACI GRCGYC 1 cut(s) 315
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 75, 79, 100
BstDEI CTNAG 5 cut(s) 19, 43, 209, 285, 620
BstF5I GGATG 3 cut(s) 386, 591, 661
BstFNI CGCG 1 cut(s) 305
BstKTI GATC 5 cut(s) 68, 373, 377, 472, 532
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 5 cut(s) 65, 370, 374, 469, 529
BstMCI CGRYCG 1 cut(s) 377
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 1 cut(s) 503
BstNSI RCATGY 1 cut(s) 439
BstSCI CCNGG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 432
BstUI CGCG 1 cut(s) 305
BstV1I GCAGC 2 cut(s) 109, 451
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 2 cut(s) 65, 529
BstYI RGATCY 2 cut(s) 65, 529
Bsu15I ATCGAT 2 cut(s) 373, 377
BsuTUI ATCGAT 2 cut(s) 373, 377
BtrI CACGTC 1 cut(s) 487
BtsCI GGATG 3 cut(s) 386, 591, 661
BtsI GCAGTG 1 cut(s) 673
BtsIMutI CAGTG 2 cut(s) 331, 673
Cac8I GCNNGC 3 cut(s) 75, 79, 100
ClaI ATCGAT 2 cut(s) 373, 377
Csp6I GTAC 3 cut(s) 172, 250, 568
CviAII CATG 2 cut(s) 49, 436
CviJI RGCY 5 cut(s) 47, 136, 213, 556, 625
CviKI_1 RGCY 5 cut(s) 47, 136, 213, 556, 625
CviQI GTAC 3 cut(s) 172, 250, 568
DdeI CTNAG 5 cut(s) 19, 43, 209, 285, 620
DpnI GATC 5 cut(s) 67, 372, 376, 471, 531
DpnII GATC 5 cut(s) 65, 370, 374, 469, 529
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
EciI GGCGGA 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 501
FaeI CATG 2 cut(s) 52, 439
FalI AAGNNNNNCTT 4 cut(s) 281, 313, 613, 645
FaqI GGGAC 2 cut(s) 602, 658
FatI CATG 2 cut(s) 48, 435
FauI CCCGC 2 cut(s) 44, 107
FblI GTMKAC 1 cut(s) 383
Fnu4HI GCNGC 3 cut(s) 96, 123, 465
FokI GGATG 3 cut(s) 373, 578, 668
Fsp4HI GCNGC 3 cut(s) 96, 123, 465
GluI GCNGC 3 cut(s) 96, 123, 465
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 2 cut(s) 52, 439
HincII GTYRAC 2 cut(s) 384, 525
HindII GTYRAC 2 cut(s) 384, 525
HinfI GANTC 2 cut(s) 31, 539
HphI GGTGA 5 cut(s) 142, 181, 262, 370, 614
Hpy166II GTNNAC 3 cut(s) 150, 384, 525
Hpy188I TCNGA 2 cut(s) 22, 411
Hpy188III TCNNGA 1 cut(s) 338
Hpy8I GTNNAC 3 cut(s) 150, 384, 525
Hpy99I CGWCG 2 cut(s) 320, 488
HpyCH4III ACNGT 4 cut(s) 200, 403, 479, 567
HpyCH4IV ACGT 2 cut(s) 315, 486
HpyCH4V TGCA 4 cut(s) 8, 56, 81, 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpyF3I CTNAG 5 cut(s) 19, 43, 209, 285, 620
HpySE526I ACGT 2 cut(s) 315, 486
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 2 cut(s) 52, 439
Kzo9I GATC 5 cut(s) 65, 370, 374, 469, 529
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 5 cut(s) 87, 341, 474, 488, 515
Lsp1109I GCAGC 2 cut(s) 109, 451
LweI GCATC 1 cut(s) 646
MaeII ACGT 2 cut(s) 315, 486
MaeIII GTNAC 2 cut(s) 358, 454
MalI GATC 5 cut(s) 67, 372, 376, 471, 531
MbiI CCGCTC 1 cut(s) 39
MboI GATC 5 cut(s) 65, 370, 374, 469, 529
MboII GAAGA 3 cut(s) 289, 301, 406
MflI RGATCY 2 cut(s) 65, 529
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 3 cut(s) 215, 449, 688
MluI ACGCGT 1 cut(s) 303
MnlI CCTC 8 cut(s) 28, 266, 272, 284, 419, 439, 578, 598
MroXI GAANNNNTTC 1 cut(s) 296
MslI CAYNNNNRTG 1 cut(s) 600
MspA1I CMGCKG 1 cut(s) 600
MspR9I CCNGG 1 cut(s) 503
MvaI CCWGG 1 cut(s) 503
MvnI CGCG 1 cut(s) 305
MwoI GCNNNNNNNGC 1 cut(s) 53
NdeII GATC 5 cut(s) 65, 370, 374, 469, 529
NlaIII CATG 2 cut(s) 52, 439
NmuCI GTSAC 2 cut(s) 358, 454
NspI RCATGY 1 cut(s) 439
NspV TTCGAA 1 cut(s) 167
OliI CACNNNNGTG 1 cut(s) 600
PciI ACATGT 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 296
PfeI GAWTC 2 cut(s) 31, 539
PflFI GACNNNGTC 2 cut(s) 351, 488
PfoI TCCNGGA 1 cut(s) 501
PkrI GCNGC 3 cut(s) 97, 124, 466
Ple19I CGATCG 1 cut(s) 377
PscI ACATGT 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 501
PspGI CCWGG 1 cut(s) 501
PsuI RGATCY 2 cut(s) 65, 529
PsyI GACNNNGTC 2 cut(s) 351, 488
PvuI CGATCG 1 cut(s) 377
RsaI GTAC 3 cut(s) 173, 251, 569
RsaNI GTAC 3 cut(s) 172, 250, 568
RseI CAYNNNNRTG 1 cut(s) 600
SalI GTCGAC 1 cut(s) 382
SatI GCNGC 3 cut(s) 96, 123, 465
Sau3AI GATC 5 cut(s) 65, 370, 374, 469, 529
ScrFI CCNGG 1 cut(s) 503
SduI GDGCHC 1 cut(s) 432
SetI ASST 6 cut(s) 20, 258, 318, 360, 489, 609
SfaNI GCATC 1 cut(s) 646
SfuI TTCGAA 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 600
Sse9I AATT 3 cut(s) 215, 449, 688
SsiI CCGC 5 cut(s) 37, 71, 96, 100, 600
SspI AATATT 1 cut(s) 549
StyD4I CCNGG 1 cut(s) 501
TaaI ACNGT 4 cut(s) 200, 403, 479, 567
TaiI ACGT 2 cut(s) 318, 489
TaqI TCGA 7 cut(s) 167, 318, 373, 377, 383, 424, 483
TasI AATT 3 cut(s) 215, 449, 688
TatI WGTACW 1 cut(s) 171
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 2 cut(s) 31, 539
TscAI CASTG 2 cut(s) 331, 680
TseFI GTSAC 2 cut(s) 358, 454
TseI GCWGC 2 cut(s) 122, 464
Tsp45I GTSAC 2 cut(s) 358, 454
TspDTI ATGAA 1 cut(s) 566
TspRI CASTG 2 cut(s) 331, 680
Tth111I GACNNNGTC 2 cut(s) 351, 488
XapI RAATTY 1 cut(s) 449
XceI RCATGY 1 cut(s) 439
XmiI GTMKAC 1 cut(s) 383
XmnI GAANNNNTTC 1 cut(s) 296
ZraI GACGTC 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.