pycom05g03040

Family of unknown function (DUF5306)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016755
Physical Location & Seq
Forward (+)
3527367 .. 3527987
621 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 369 bp
ATGGAGTACTCACTGTCGCATAAAGTATCAGCTGATGAGGAGGAAGAGTTGATGGACAAGAGAAGTTACTATATATTTCCATGCCACTTCCTTGAAGAAGCCGTCCGGGCTTTTTTCAAGTGTTTGGGCATTGAGACTAAATCTCAGGAGGACAAAGACACTGATGAAATAAACCCCGAGAAAATTACTCCTCCAACCTCAGAACAGCTACTTGATGGTGCAGTTCCATCACTTCTGATGACATACGGTGATCCTCCATCTTCATCAGCTACAGAAACTGCAGACGAAGCTGCTGCAATTACAAGGATAAAGGTGAGCGCTCGAGAAAGACCTGGACTAAGCACAGGGAAAGGTGGGAAAACTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF5306

Protein Analysis

123

Amino Acids

13.45

Weight (kDa)

4.68

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pep1_7 PF17232 13 - 59 5.9e-09 Elicitor peptide 1-7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016755)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12531
malus_domestica MD00G1161700.v1.1 MD10G1043000.v1.1
prunus_persica Prupe.8G048500_v2.0.a1
pyrus_communis pycom05g03040
rosa_laevigata RLG00000013646
rosa_roxburghii Rroxscaffold_1G00008980 Rroxscaffold_3G00220660 Rroxscaffold_7G00195720
rosa_rugosa Rorug06G0072300
rosa_samantha Rh6AG187900 Rh6BG191300 Rh6CG190300 Rh6DG181900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 245
AfaI GTAC 1 cut(s) 8
AfeI AGCGCT 1 cut(s) 319
AgsI TTSAA 2 cut(s) 95, 118
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 4 cut(s) 32, 208, 269, 290
AluI AGCT 4 cut(s) 32, 208, 269, 290
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 1 cut(s) 245
AlwNI CAGNNNCTG 1 cut(s) 278
Ama87I CYCGRG 2 cut(s) 176, 321
Aor51HI AGCGCT 1 cut(s) 319
ApeKI GCWGC 2 cut(s) 290, 293
AspLEI GCGC 1 cut(s) 320
AsuC2I CCSGG 1 cut(s) 107
AsuHPI GGTGA 2 cut(s) 260, 325
AvaI CYCGRG 2 cut(s) 176, 321
BbvI GCAGC 2 cut(s) 277, 280
BccI CCATC 4 cut(s) 46, 209, 235, 265
BceAI ACGGC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 1 cut(s) 333
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 128
BfmI CTRYAG 2 cut(s) 270, 279
BfoI RGCGCY 1 cut(s) 321
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 2 cut(s) 291, 294
BlsI GCNGC 2 cut(s) 292, 295
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 2 cut(s) 107, 333
BmeT110I CYCGRG 2 cut(s) 176, 321
BmrFI CCNGG 2 cut(s) 107, 333
BpuMI CCSGG 1 cut(s) 107
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
BseBI CCWGG 1 cut(s) 333
BseMII CTCAG 2 cut(s) 158, 213
BseRI GAGGAG 2 cut(s) 53, 180
BseXI GCAGC 2 cut(s) 277, 280
BsgI GTGCAG 1 cut(s) 240
BsiHKCI CYCGRG 2 cut(s) 176, 321
BsiSI CCGG 1 cut(s) 106
BsmAI GTCTC 1 cut(s) 128
BsoBI CYCGRG 2 cut(s) 176, 321
Bsp143I GATC 1 cut(s) 250
BspCNI CTCAG 2 cut(s) 157, 212
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 1 cut(s) 245
BssMI GATC 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 2 cut(s) 15, 248
Bst6I CTCTTC 1 cut(s) 39
BstDEI CTNAG 3 cut(s) 144, 199, 338
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 1 cut(s) 253
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 1 cut(s) 250
BstMWI GCNNNNNNNGC 2 cut(s) 107, 287
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 2 cut(s) 105, 331
BstSFI CTRYAG 2 cut(s) 270, 279
BstV1I GCAGC 2 cut(s) 277, 280
BtsIMutI CAGTG 2 cut(s) 11, 159
CaiI CAGNNNCTG 1 cut(s) 278
CfoI GCGC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 7
CviAII CATG 1 cut(s) 81
CviJI RGCY 6 cut(s) 32, 101, 110, 208, 269, 290
CviKI_1 RGCY 6 cut(s) 32, 101, 110, 208, 269, 290
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 3 cut(s) 144, 199, 338
DpnI GATC 1 cut(s) 252
DpnII GATC 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco47III AGCGCT 1 cut(s) 319
Eco88I CYCGRG 2 cut(s) 176, 321
EcoRII CCWGG 1 cut(s) 331
FaeI CATG 1 cut(s) 84
FaiI YATR 5 cut(s) 21, 72, 74, 82, 244
FatI CATG 1 cut(s) 80
Fnu4HI GCNGC 2 cut(s) 291, 294
Fsp4HI GCNGC 2 cut(s) 291, 294
GlaI GCGC 1 cut(s) 319
GluI GCNGC 2 cut(s) 291, 294
HaeII RGCGCY 1 cut(s) 321
HapII CCGG 1 cut(s) 106
HhaI GCGC 1 cut(s) 320
Hin1II CATG 1 cut(s) 84
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HpaII CCGG 1 cut(s) 106
HphI GGTGA 2 cut(s) 260, 325
Hpy188I TCNGA 2 cut(s) 202, 237
Hpy188III TCNNGA 2 cut(s) 146, 323
HpyCH4III ACNGT 2 cut(s) 15, 248
HpyCH4V TGCA 3 cut(s) 221, 281, 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 287
HpyF3I CTNAG 3 cut(s) 144, 199, 338
Hsp92II CATG 1 cut(s) 84
HspAI GCGC 1 cut(s) 318
Kzo9I GATC 1 cut(s) 250
LpnPI CCDG 5 cut(s) 119, 131, 318, 330, 345
Lsp1109I GCAGC 2 cut(s) 277, 280
MaeIII GTNAC 1 cut(s) 65
MalI GATC 1 cut(s) 252
MboI GATC 1 cut(s) 250
MboII GAAGA 3 cut(s) 56, 107, 252
MluCI AATT 3 cut(s) 183, 297, 364
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 6 cut(s) 31, 34, 142, 201, 208, 264
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 2 cut(s) 107, 333
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 2 cut(s) 107, 287
NciI CCSGG 1 cut(s) 107
NdeII GATC 1 cut(s) 250
NlaIII CATG 1 cut(s) 84
PaeR7I CTCGAG 1 cut(s) 321
PkrI GCNGC 2 cut(s) 292, 295
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PstI CTGCAG 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SatI GCNGC 2 cut(s) 291, 294
Sau3AI GATC 1 cut(s) 250
ScaI AGTACT 1 cut(s) 8
ScrFI CCNGG 2 cut(s) 107, 333
SetI ASST 8 cut(s) 34, 200, 210, 271, 292, 315, 334, 355
SfcI CTRYAG 2 cut(s) 270, 279
Sfr274I CTCGAG 1 cut(s) 321
SlaI CTCGAG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 3 cut(s) 183, 297, 364
StyD4I CCNGG 2 cut(s) 105, 331
TaaI ACNGT 2 cut(s) 15, 248
TaqI TCGA 1 cut(s) 322
TasI AATT 3 cut(s) 183, 297, 364
TatI WGTACW 1 cut(s) 6
TscAI CASTG 2 cut(s) 18, 166
TseI GCWGC 2 cut(s) 290, 293
TspDTI ATGAA 2 cut(s) 180, 252
TspRI CASTG 2 cut(s) 18, 166
XhoI CTCGAG 1 cut(s) 321
ZrmI AGTACT 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.