Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0016570

Physical Location & Seq

Genomic Coordinates Forward (+)

4595612 .. 4598267

Gene Structure 2656 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g03990.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1248 bp

ATGCCATTACCCACCACAATCATAACCATAACACCACCGTCCACCTCCTGCCCTGCTCCAATTTTCCCACCAAATCGCCCTCATTTCCCGCCCAGAAATCTGTTTACTACAAAAACCATCCTCCACAACCACAGACCAGCAATGGCAAATCTTTCTGGGTTCGGTTTCAGTATTGGGTCCCGACTGGTGGCTTCTTCCGGTACTAGTGGCAGTGTTGCCGATGATGAGGTGCGCACCCTGATTGAGTATGCGGCCGGCGAGGGAGGTGCCACTGTGGGAGATGATCTGGTGCTTCTGTTCCACCAATTACAGTACGCCTGCAAGAGAATAGCAGCTCTTGTAGCTTCTCCTTTCAATTCCAGCCTTGGGAAGCACAAGGGTCTCTCTGGCGGCGGCGCTTCGGCTGCTGGGTCAGGCCGGGATGCACCGAAGCCTCTTGATATCGTTTCGAATGAAATCATCTTGTCCTCTCTTAAAAATTCTGGAAAAGTAGCTGTCATGGCTTCAGAAGAAGATGATACTCCAATTTGGATAACCGATGATGGCCCATTTGTGGTGGTAACGGATCCTCTAGATGGTTCTCGAAATATTGATGCGTCCATTCCCACTGGAACCATTTTTGGGATTTATAAACGCCTTCCGGAGCTGGATCATCTACCAAAGGAGGAGAAGGCTTTGCTGAATTCACTCCAGAAGGGAACTAATCTTGTTGCTGCCGGCTATGTTCTTTATTCATCTGCCACAATATTCTGCACCAGCTTTGGTTCAGGAACACATGCATTCACGCTGGATCATTCAACAGGAGATTTCATTCTCACACACCCAAGCTTAAAAATTCCTCCTCGAGGGCAAATATATTCTGTAAATGATGCTCGATATTTTGACTGGCCCAATGGATTAAGGCAATACATAGACACTGTGAGACAAGGGAAAGGCAGATACCCCAAGAAGTACTCTGCGCGTTATATCTGTTCTCTGGTAGCAGATTTTCATCGAACTCTACTGTATGGTGGAGTGACAATGAATCCAAGGGACCACCTTCGTCTGGTTTACGAAGCCAACCCTCTTAGTTTTCTGGTGGAGCAAGCCGGGGGCAAATGTTCTGATGGTAAAAGTAGAATTCTATCGATTCAGCCAGTCAAGCTGCACCAAAGGCTGCCTCTGTTCCTGGGGAGTTTGGAGGACATGGAAGAGTTAGAGAGTTACGGAGATGTGCAACAGAAAGTGAACCCAGGATACGAGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko04152 ko04910 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map04152 map04910

Pfam Domains

Protein Families

FBPase

Protein Analysis

416

Amino Acids

44.92

Weight (kDa)

6.86

Isoelectric Point (pI)

37.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FBPase	PF00316	94 - 275	2.2e-37	Fructose-1-6-bisphosphatase, N-terminal domain
FBPase_C	PF18913	279 - 402	7.2e-38	Fructose-1-6-bisphosphatase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016570)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64380
fragaria_vesca	FvH4_2g11590
malus_domestica	MD05G1048200.v1.1
prunus_persica	Prupe.8G070500_v2.0.a1
pyrus_communis	pycom05g03990
rosa_chinensis	RchiOBHm_Chr6g0270781
rosa_laevigata	RLG00000013769
rosa_multiflora	Rmu_sc0000642.1_g000021
rosa_roxburghii	Rroxscaffold_7G00197600
rosa_rugosa	Rorug06G0056600
rosa_samantha	Rh6AG175800 Rh6BG179000 Rh6DG167600
rosa_wichuraiana	Rw6G014970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	630
AbsI	CCTCGAGG	1 cut(s)	843
Acc16I	TGCGCA	1 cut(s)	233
AccB1I	GGYRCC	1 cut(s)	266
AccII	CGCG	1 cut(s)	961
AccIII	TCCGGA	1 cut(s)	640
AciI	CCGC	4 cut(s)	89, 251, 390, 393
AclWI	GGATC	4 cut(s)	560, 573, 657, 798
AcoI	YGGCCR	1 cut(s)	252
AcsI	RAATTY	4 cut(s)	478, 682, 834, 1119
AcuI	CTGAAG	1 cut(s)	489
AfaI	GTAC	3 cut(s)	202, 314, 953
AfiI	CCNNNNNNNGG	7 cut(s)	187, 366, 553, 575, 621, 845, 1045
AgsI	TTSAA	2 cut(s)	355, 798
AhlI	ACTAGT	1 cut(s)	203
AjnI	CCWGG	2 cut(s)	1167, 1231
AluBI	AGCT	7 cut(s)	335, 344, 494, 646, 759, 828, 1144
AluI	AGCT	7 cut(s)	335, 344, 494, 646, 759, 828, 1144
Alw26I	GTCTC	2 cut(s)	386, 916
AlwI	GGATC	4 cut(s)	560, 573, 657, 798
Ama87I	CYCGRG	1 cut(s)	843
Aor13HI	TCCGGA	1 cut(s)	640
AoxI	GGCC	4 cut(s)	252, 415, 544, 887
ApeKI	GCWGC	5 cut(s)	332, 404, 713, 1144, 1156
ApoI	RAATTY	4 cut(s)	478, 682, 834, 1119
AspLEI	GCGC	3 cut(s)	234, 398, 961
AspS9I	GGNCC	4 cut(s)	177, 545, 888, 1033
AsuC2I	CCSGG	2 cut(s)	419, 1090
AsuII	TTCGAA	1 cut(s)	449
AvaI	CYCGRG	1 cut(s)	843
AvaII	GGWCC	2 cut(s)	177, 1033
BamHI	GGATCC	1 cut(s)	565
BanI	GGYRCC	1 cut(s)	266
BbvI	GCAGC	5 cut(s)	344, 391, 700, 1131, 1143
BccI	CCATC	4 cut(s)	125, 536, 569, 1100
BcgI	CGANNNNNNTGC	2 cut(s)	248, 282
BciT130I	CCWGG	2 cut(s)	1169, 1233
BciVI	GTATCC	1 cut(s)	1229
BcnI	CCSGG	2 cut(s)	419, 1090
BcoDI	GTCTC	2 cut(s)	386, 916
BcuI	ACTAGT	1 cut(s)	203
BfaI	CTAG	2 cut(s)	204, 572
BfoI	RGCGCY	1 cut(s)	399
BfuI	GTATCC	1 cut(s)	1229
BisI	GCNGC	8 cut(s)	252, 333, 391, 394, 405, 714, 1145, 1157
BlsI	GCNGC	8 cut(s)	253, 334, 392, 395, 406, 715, 1146, 1158
BmcAI	AGTACT	1 cut(s)	953
Bme1390I	CCNGG	4 cut(s)	419, 1090, 1169, 1233
Bme18I	GGWCC	2 cut(s)	177, 1033
BmeT110I	CYCGRG	1 cut(s)	843
BmgT120I	GGNCC	4 cut(s)	177, 545, 888, 1033
BmiI	GGNNCC	6 cut(s)	178, 179, 268, 567, 613, 1034
BmrFI	CCNGG	4 cut(s)	419, 1090, 1169, 1233
BmsI	GCATC	3 cut(s)	412, 583, 859
BpmI	CTGGAG	1 cut(s)	674
Bpu14I	TTCGAA	1 cut(s)	449
BpuMI	CCSGG	2 cut(s)	419, 1090
Bsa29I	ATCGAT	1 cut(s)	1127
BsaBI	GATNNNNATC	1 cut(s)	990
BsaI	GGTCTC	1 cut(s)	386
BsaJI	CCNNGG	5 cut(s)	364, 1028, 1089, 1168, 1231
BsaWI	WCCGGW	2 cut(s)	197, 640
Bsc4I	CCNNNNNNNGG	7 cut(s)	187, 366, 553, 575, 621, 845, 1045
Bse118I	RCCGGY	2 cut(s)	254, 716
Bse1I	ACTGG	4 cut(s)	189, 613, 890, 1136
Bse3DI	GCAATG	1 cut(s)	147
Bse8I	GATNNNNATC	1 cut(s)	990
BseAI	TCCGGA	1 cut(s)	640
BseBI	CCWGG	2 cut(s)	1169, 1233
BseCI	ATCGAT	1 cut(s)	1127
BseDI	CCNNGG	5 cut(s)	364, 1028, 1089, 1168, 1231
BseGI	GGATG	2 cut(s)	117, 427
BseJI	GATNNNNATC	1 cut(s)	990
BseLI	CCNNNNNNNGG	7 cut(s)	187, 366, 553, 575, 621, 845, 1045
BseMI	GCAATG	1 cut(s)	147
BseNI	ACTGG	4 cut(s)	189, 613, 890, 1136
BseRI	GAGGAG	2 cut(s)	680, 831
BseX3I	CGGCCG	1 cut(s)	252
BseXI	GCAGC	5 cut(s)	344, 391, 700, 1131, 1143
BseYI	CCCAGC	1 cut(s)	407
BsgI	GTGCAG	2 cut(s)	736, 1130
Bsh1236I	CGCG	1 cut(s)	961
Bsh1285I	CGRYCG	1 cut(s)	255
BshFI	GGCC	4 cut(s)	254, 417, 546, 889
BshNI	GGYRCC	1 cut(s)	266
BshVI	ATCGAT	1 cut(s)	1127
BsiEI	CGRYCG	1 cut(s)	255
BsiHKCI	CYCGRG	1 cut(s)	843
BsiSI	CCGG	6 cut(s)	198, 255, 418, 641, 717, 1089
BslFI	GGGAC	2 cut(s)	163, 1046
BslI	CCNNNNNNNGG	7 cut(s)	187, 366, 553, 575, 621, 845, 1045
BsmAI	GTCTC	2 cut(s)	386, 916
BsmFI	GGGAC	2 cut(s)	163, 1046
BsmI	GAATGC	1 cut(s)	779
BsnI	GGCC	4 cut(s)	254, 417, 546, 889
Bso31I	GGTCTC	1 cut(s)	386
BsoBI	CYCGRG	1 cut(s)	843
Bsp119I	TTCGAA	1 cut(s)	449
Bsp13I	TCCGGA	1 cut(s)	640
Bsp143I	GATC	4 cut(s)	283, 565, 649, 790
BspACI	CCGC	4 cut(s)	89, 251, 390, 393
BspANI	GGCC	4 cut(s)	254, 417, 546, 889
BspDI	ATCGAT	1 cut(s)	1127
BspEI	TCCGGA	1 cut(s)	640
BspFNI	CGCG	1 cut(s)	961
BspLI	GGNNCC	6 cut(s)	178, 179, 268, 567, 613, 1034
BspPI	GGATC	4 cut(s)	560, 573, 657, 798
BspT104I	TTCGAA	1 cut(s)	449
BspT107I	GGYRCC	1 cut(s)	266
BspTNI	GGTCTC	1 cut(s)	386
BsrDI	GCAATG	1 cut(s)	147
BsrFI	RCCGGY	2 cut(s)	254, 716
BsrI	ACTGG	4 cut(s)	189, 613, 890, 1136
BssAI	RCCGGY	2 cut(s)	254, 716
BssECI	CCNNGG	5 cut(s)	364, 1028, 1089, 1168, 1231
BssMI	GATC	4 cut(s)	283, 565, 649, 790
BssT1I	CCWWGG	2 cut(s)	364, 1028
Bst2UI	CCWGG	2 cut(s)	1169, 1233
Bst4CI	ACNGT	5 cut(s)	39, 274, 312, 919, 1005
Bst6I	CTCTTC	1 cut(s)	1185
BstBI	TTCGAA	1 cut(s)	449
BstC8I	GCNNGC	4 cut(s)	256, 319, 718, 1086
BstDEI	CTNAG	1 cut(s)	1067
BstENI	CCTNNNNNAGG	1 cut(s)	843
BstF5I	GGATG	2 cut(s)	117, 427
BstFNI	CGCG	1 cut(s)	961
BstH2I	RGCGCY	1 cut(s)	399
BstHHI	GCGC	3 cut(s)	234, 398, 961
BstKTI	GATC	4 cut(s)	286, 568, 652, 793
BstMAI	GTCTC	2 cut(s)	386, 916
BstMBI	GATC	4 cut(s)	283, 565, 649, 790
BstMCI	CGRYCG	1 cut(s)	255
BstMWI	GCNNNNNNNGC	5 cut(s)	341, 404, 500, 1141, 1153
BstNI	CCWGG	2 cut(s)	1169, 1233
BstNSI	RCATGY	1 cut(s)	779
BstSCI	CCNGG	4 cut(s)	417, 1088, 1167, 1231
BstUI	CGCG	1 cut(s)	961
BstV1I	GCAGC	5 cut(s)	344, 391, 700, 1131, 1143
BstX2I	RGATCY	1 cut(s)	565
BstYI	RGATCY	1 cut(s)	565
BstZI	CGGCCG	1 cut(s)	252
Bsu15I	ATCGAT	1 cut(s)	1127
BsuI	GTATCC	1 cut(s)	1229
BsuRI	GGCC	4 cut(s)	254, 417, 546, 889
BsuTUI	ATCGAT	1 cut(s)	1127
BtsCI	GGATG	2 cut(s)	117, 427
BtsI	GCAGTG	1 cut(s)	217
BtsIMutI	CAGTG	4 cut(s)	217, 270, 606, 915
Cac8I	GCNNGC	4 cut(s)	256, 319, 718, 1086
CfoI	GCGC	3 cut(s)	234, 398, 961
Cfr10I	RCCGGY	2 cut(s)	254, 716
Cfr13I	GGNCC	4 cut(s)	177, 545, 888, 1033
ClaI	ATCGAT	1 cut(s)	1127
CseI	GACGC	1 cut(s)	585
Csp6I	GTAC	3 cut(s)	201, 313, 952
CviAII	CATG	3 cut(s)	499, 776, 1186
CviQI	GTAC	3 cut(s)	201, 313, 952
DdeI	CTNAG	1 cut(s)	1067
DpnI	GATC	4 cut(s)	285, 567, 651, 792
DpnII	GATC	4 cut(s)	283, 565, 649, 790
EaeI	YGGCCR	1 cut(s)	252
EagI	CGGCCG	1 cut(s)	252
Eam1104I	CTCTTC	1 cut(s)	1185
EarI	CTCTTC	1 cut(s)	1185
EclXI	CGGCCG	1 cut(s)	252
Eco130I	CCWWGG	2 cut(s)	364, 1028
Eco31I	GGTCTC	1 cut(s)	386
Eco32I	GATATC	1 cut(s)	442
Eco47I	GGWCC	2 cut(s)	177, 1033
Eco52I	CGGCCG	1 cut(s)	252
Eco57I	CTGAAG	1 cut(s)	489
Eco88I	CYCGRG	1 cut(s)	843
EcoNI	CCTNNNNNAGG	1 cut(s)	843
EcoO109I	RGGNCCY	1 cut(s)	177
EcoRI	GAATTC	2 cut(s)	682, 1119
EcoRII	CCWGG	2 cut(s)	1167, 1231
EcoRV	GATATC	1 cut(s)	442
EcoT14I	CCWWGG	2 cut(s)	364, 1028
EcoT22I	ATGCAT	1 cut(s)	781
ErhI	CCWWGG	2 cut(s)	364, 1028
FaeI	CATG	3 cut(s)	502, 779, 1189
FaqI	GGGAC	2 cut(s)	163, 1046
FatI	CATG	3 cut(s)	498, 775, 1185
FauI	CCCGC	1 cut(s)	96
Fnu4HI	GCNGC	8 cut(s)	252, 333, 391, 394, 405, 714, 1145, 1157
FokI	GGATG	2 cut(s)	104, 434
Fsp4HI	GCNGC	8 cut(s)	252, 333, 391, 394, 405, 714, 1145, 1157
FspAI	RTGCGCAY	1 cut(s)	233
FspBI	CTAG	2 cut(s)	204, 572
FspI	TGCGCA	1 cut(s)	233
GlaI	GCGC	3 cut(s)	233, 397, 960
GluI	GCNGC	8 cut(s)	252, 333, 391, 394, 405, 714, 1145, 1157
GsaI	CCCAGC	1 cut(s)	411
GsuI	CTGGAG	1 cut(s)	674
HaeII	RGCGCY	1 cut(s)	399
HaeIII	GGCC	4 cut(s)	254, 417, 546, 889
HapII	CCGG	6 cut(s)	198, 255, 418, 641, 717, 1089
HgaI	GACGC	1 cut(s)	585
HhaI	GCGC	3 cut(s)	234, 398, 961
Hin1II	CATG	3 cut(s)	502, 779, 1189
Hin6I	GCGC	3 cut(s)	232, 396, 959
HinP1I	GCGC	3 cut(s)	232, 396, 959
HindIII	AAGCTT	1 cut(s)	826
HinfI	GANTC	2 cut(s)	1024, 1129
HpaII	CCGG	6 cut(s)	198, 255, 418, 641, 717, 1089
Hpy166II	GTNNAC	4 cut(s)	42, 105, 1051, 1228
Hpy188I	TCNGA	2 cut(s)	508, 1105
Hpy188III	TCNNGA	8 cut(s)	180, 437, 483, 572, 582, 641, 691, 768
Hpy8I	GTNNAC	4 cut(s)	42, 105, 1051, 1228
HpyAV	CCTTC	4 cut(s)	647, 664, 688, 1049
HpyCH4III	ACNGT	5 cut(s)	39, 274, 312, 919, 1005
HpyCH4V	TGCA	6 cut(s)	321, 425, 753, 779, 1147, 1216
HpyF10VI	GCNNNNNNNGC	5 cut(s)	341, 404, 500, 1141, 1153
HpyF3I	CTNAG	1 cut(s)	1067
Hsp92II	CATG	3 cut(s)	502, 779, 1189
HspAI	GCGC	3 cut(s)	232, 396, 959
KflI	GGGWCCC	1 cut(s)	177
Kpn2I	TCCGGA	1 cut(s)	640
KroI	GCCGGC	2 cut(s)	254, 716
KroNI	GCCGGC	2 cut(s)	256, 718
Kzo9I	GATC	4 cut(s)	283, 565, 649, 790
LmnI	GCTCC	3 cut(s)	61, 643, 1081
Lsp1109I	GCAGC	5 cut(s)	344, 391, 700, 1131, 1143
LweI	GCATC	3 cut(s)	412, 583, 859
MaeI	CTAG	2 cut(s)	204, 572
MaeIII	GTNAC	3 cut(s)	559, 1015, 1202
MalI	GATC	4 cut(s)	285, 567, 651, 792
MboI	GATC	4 cut(s)	283, 565, 649, 790
MboII	GAAGA	4 cut(s)	186, 521, 524, 1202
MflI	RGATCY	1 cut(s)	565
MluCI	AATT	8 cut(s)	60, 305, 355, 478, 525, 682, 834, 1119
Mph1103I	ATGCAT	1 cut(s)	781
MroI	TCCGGA	1 cut(s)	640
MroNI	GCCGGC	2 cut(s)	254, 716
MseI	TTAA	3 cut(s)	474, 830, 899
MspI	CCGG	6 cut(s)	198, 255, 418, 641, 717, 1089
MspR9I	CCNGG	4 cut(s)	419, 1090, 1169, 1233
Mva1269I	GAATGC	1 cut(s)	779
MvaI	CCWGG	2 cut(s)	1169, 1233
MvnI	CGCG	1 cut(s)	961
MwoI	GCNNNNNNNGC	5 cut(s)	341, 404, 500, 1141, 1153
NaeI	GCCGGC	2 cut(s)	256, 718
NciI	CCSGG	2 cut(s)	419, 1090
NdeII	GATC	4 cut(s)	283, 565, 649, 790
NgoMIV	GCCGGC	2 cut(s)	254, 716
NlaIII	CATG	3 cut(s)	502, 779, 1189
NlaIV	GGNNCC	6 cut(s)	178, 179, 268, 567, 613, 1034
NmuCI	GTSAC	1 cut(s)	1015
NsbI	TGCGCA	1 cut(s)	233
NsiI	ATGCAT	1 cut(s)	781
NspI	RCATGY	1 cut(s)	779
NspV	TTCGAA	1 cut(s)	449
PaeR7I	CTCGAG	1 cut(s)	843
PctI	GAATGC	1 cut(s)	779
PdiI	GCCGGC	2 cut(s)	256, 718
PfeI	GAWTC	2 cut(s)	1024, 1129
PkrI	GCNGC	8 cut(s)	253, 334, 392, 395, 406, 715, 1146, 1158
PpuMI	RGGWCCY	1 cut(s)	177
PsiI	TTATAA	1 cut(s)	630
Psp5II	RGGWCCY	1 cut(s)	177
Psp6I	CCWGG	2 cut(s)	1167, 1231
PspFI	CCCAGC	1 cut(s)	407
PspGI	CCWGG	2 cut(s)	1167, 1231
PspN4I	GGNNCC	6 cut(s)	178, 179, 268, 567, 613, 1034
PspPI	GGNCC	4 cut(s)	177, 545, 888, 1033
PspPPI	RGGWCCY	1 cut(s)	177
PspXI	VCTCGAGB	1 cut(s)	843
PsrI	GAACNNNNNNTAC	1 cut(s)	1220
PsuI	RGATCY	1 cut(s)	565
RsaI	GTAC	3 cut(s)	202, 314, 953
RsaNI	GTAC	3 cut(s)	201, 313, 952
SaqAI	TTAA	3 cut(s)	474, 830, 899
SatI	GCNGC	8 cut(s)	252, 333, 391, 394, 405, 714, 1145, 1157
Sau3AI	GATC	4 cut(s)	283, 565, 649, 790
Sau96I	GGNCC	4 cut(s)	177, 545, 888, 1033
ScaI	AGTACT	1 cut(s)	953
ScrFI	CCNGG	4 cut(s)	419, 1090, 1169, 1233
SfaNI	GCATC	3 cut(s)	412, 583, 859
Sfr274I	CTCGAG	1 cut(s)	843
SfuI	TTCGAA	1 cut(s)	449
SinI	GGWCC	2 cut(s)	177, 1033
SlaI	CTCGAG	1 cut(s)	843
SmlI	CTYRAG	1 cut(s)	843
SmoI	CTYRAG	1 cut(s)	843
SpeI	ACTAGT	1 cut(s)	203
Sse9I	AATT	8 cut(s)	60, 305, 355, 478, 525, 682, 834, 1119
SsiI	CCGC	4 cut(s)	89, 251, 390, 393
SspI	AATATT	2 cut(s)	589, 747
SspMI	CTAG	2 cut(s)	204, 572
StyD4I	CCNGG	4 cut(s)	417, 1088, 1167, 1231
StyI	CCWWGG	2 cut(s)	364, 1028
TaaI	ACNGT	5 cut(s)	39, 274, 312, 919, 1005
TaqI	TCGA	6 cut(s)	449, 583, 844, 874, 994, 1127
TasI	AATT	8 cut(s)	60, 305, 355, 478, 525, 682, 834, 1119
TatI	WGTACW	1 cut(s)	951
TauI	GCSGC	3 cut(s)	254, 393, 396
TfiI	GAWTC	2 cut(s)	1024, 1129
Tru1I	TTAA	3 cut(s)	474, 830, 899
Tru9I	TTAA	3 cut(s)	474, 830, 899
TscAI	CASTG	4 cut(s)	217, 277, 613, 922
TseFI	GTSAC	1 cut(s)	1015
TseI	GCWGC	5 cut(s)	332, 404, 713, 1144, 1156
Tsp45I	GTSAC	1 cut(s)	1015
TspDTI	ATGAA	5 cut(s)	468, 723, 799, 980, 1037
TspGWI	ACGGA	2 cut(s)	578, 1221
TspRI	CASTG	4 cut(s)	217, 277, 613, 922
VpaK11BI	GGWCC	2 cut(s)	177, 1033
XagI	CCTNNNNNAGG	1 cut(s)	843
XapI	RAATTY	4 cut(s)	478, 682, 834, 1119
XbaI	TCTAGA	1 cut(s)	571
XceI	RCATGY	1 cut(s)	779
XhoI	CTCGAG	1 cut(s)	843
XspI	CTAG	2 cut(s)	204, 572
ZrmI	AGTACT	1 cut(s)	953
Zsp2I	ATGCAT	1 cut(s)	781

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.