pycom05g04040

glutamine amidotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012575
Physical Location & Seq
Forward (+)
4673304 .. 4677630
4327 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 732 bp
ATGGGTGGGAAGAAATTCGCAGTGCTTCTGTGCGCAGAGGACACGGAGTTTGTGAAAAAGACGTACGGGGGATATTTTGGGGTGTATCTGAGAATGCTGGCGGAGGAGGGGGAGGCTTGGGACTTGTACCGGGTGGCGTGCGGTGAGTTTCCGGAGGATGATGAGATTGAAAGCTACGATGGGTTTGTGATATCCGGAAGCAGCAGGGATGCCCACGGCACTGATGCCTGGATCTGCAGGCTCCTCTCCCTCCTCCAGAAATTGGATTCCATCAGGAAAAAAGTTCTCGGCATTTGCTTTGGTCATCAGATATTGAGCCGTGCATTGGGAGGAAAATCTGGCAGAGCCATCACAGGCTGGGATATCGGAATCCGAACCATCCACGTGTCATCATCTTCCAAGGCCTTATCCACTCTCAAAATTCCAGCTCTTCTCTCCATATATGAAATCCATCGAGATGAGGTGTGGGAACTGCCGCCTAAGGCTGAGTTAATTGCCTGGTCGGATAAGACTGGTGTGGAGATGTTTAAGTATGGAGATCACATGATGGGAATCCAAGGACACCCTGAGTACACCAAAGACATTCTTCTCAATCTTATCGATCACCTTGCCAAACTCGAATACATCTTGGACTCGGAAACTGAAGAATTGAAGGCCAAGGTGGAGGCATGTGAGCCAGACAGAGAGGCATGGAAGAGGGTTTGTAGAAGCTTTCTCAAGGGTGAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GATase

Protein Analysis

244

Amino Acids

27.37

Weight (kDa)

5.46

Isoelectric Point (pI)

34.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 53 - 192 6.3e-14 Glutamine amidotransferase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 34
AccB7I CCANNNNNTGG 1 cut(s) 262
AccIII TCCGGA 2 cut(s) 151, 194
AciI CCGC 3 cut(s) 101, 141, 476
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 2 cut(s) 14, 420
AcuI CTGAAG 1 cut(s) 663
AcvI CACGTG 1 cut(s) 385
AfaI GTAC 3 cut(s) 65, 128, 572
AfiI CCNNNNNNNGG 2 cut(s) 262, 325
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 2 cut(s) 170, 652
AjnI CCWGG 2 cut(s) 227, 497
AluBI AGCT 3 cut(s) 174, 428, 711
AluI AGCT 3 cut(s) 174, 428, 711
AlwI GGATC 1 cut(s) 239
Aor13HI TCCGGA 2 cut(s) 151, 194
AoxI GGCC 2 cut(s) 402, 654
ApeKI GCWGC 1 cut(s) 201
ApoI RAATTY 2 cut(s) 14, 420
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
Asp700I GAANNNNTTC 1 cut(s) 14
AspLEI GCGC 1 cut(s) 35
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 2 cut(s) 155, 596
AxyI CCTNAGG 1 cut(s) 480
BbrPI CACGTG 1 cut(s) 385
BbvI GCAGC 1 cut(s) 213
BccI CCATC 6 cut(s) 173, 278, 356, 386, 459, 541
BceAI ACGGC 2 cut(s) 232, 303
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 2 cut(s) 229, 499
BcnI CCSGG 1 cut(s) 131
BfmI CTRYAG 1 cut(s) 235
BisI GCNGC 2 cut(s) 202, 476
BlsI GCNGC 2 cut(s) 203, 477
Bme1390I CCNGG 3 cut(s) 131, 229, 499
BmiI GGNNCC 1 cut(s) 242
BmrFI CCNGG 3 cut(s) 131, 229, 499
BmsI GCATC 2 cut(s) 199, 214
BpmI CTGGAG 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 1 cut(s) 131
Bsa29I ATCGAT 1 cut(s) 600
BsaAI YACGTR 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 551
BsaJI CCNNGG 4 cut(s) 214, 399, 556, 657
BsaWI WCCGGW 2 cut(s) 151, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 325
Bse1I ACTGG 1 cut(s) 517
Bse21I CCTNAGG 1 cut(s) 480
Bse8I GATNNNNATC 1 cut(s) 551
BseAI TCCGGA 2 cut(s) 151, 194
BseBI CCWGG 2 cut(s) 229, 499
BseCI ATCGAT 1 cut(s) 600
BseDI CCNNGG 4 cut(s) 214, 399, 556, 657
BseGI GGATG 3 cut(s) 163, 214, 378
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 2 cut(s) 262, 325
BseMII CTCAG 3 cut(s) 80, 477, 558
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 3 cut(s) 119, 233, 242
BseXI GCAGC 1 cut(s) 213
BseYI CCCAGC 1 cut(s) 357
BshFI GGCC 2 cut(s) 404, 656
BshVI ATCGAT 1 cut(s) 600
BsiSI CCGG 3 cut(s) 130, 152, 195
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 2 cut(s) 262, 325
BsmFI GGGAC 1 cut(s) 134
BsmI GAATGC 1 cut(s) 99
BsnI GGCC 2 cut(s) 404, 656
Bsp13I TCCGGA 2 cut(s) 151, 194
Bsp143I GATC 3 cut(s) 231, 538, 601
BspACI CCGC 3 cut(s) 101, 141, 476
BspANI GGCC 2 cut(s) 404, 656
BspCNI CTCAG 3 cut(s) 81, 478, 559
BspDI ATCGAT 1 cut(s) 600
BspEI TCCGGA 2 cut(s) 151, 194
BspLI GGNNCC 1 cut(s) 242
BspMAI CTGCAG 1 cut(s) 239
BspPI GGATC 1 cut(s) 239
BspQI GCTCTTC 1 cut(s) 435
BsrI ACTGG 1 cut(s) 517
BssECI CCNNGG 4 cut(s) 214, 399, 556, 657
BssMI GATC 3 cut(s) 231, 538, 601
BssT1I CCWWGG 3 cut(s) 399, 556, 657
Bst2UI CCWGG 2 cut(s) 229, 499
Bst6I CTCTTC 2 cut(s) 435, 689
BstBAI YACGTR 1 cut(s) 385
BstC8I GCNNGC 3 cut(s) 99, 139, 239
BstDEI CTNAG 4 cut(s) 89, 480, 486, 567
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 3 cut(s) 163, 214, 378
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 3 cut(s) 234, 541, 604
BstMBI GATC 3 cut(s) 231, 538, 601
BstNI CCWGG 2 cut(s) 229, 499
BstNSI RCATGY 1 cut(s) 672
BstSCI CCNGG 3 cut(s) 129, 227, 497
BstSFI CTRYAG 1 cut(s) 235
BstV1I GCAGC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
Bsu15I ATCGAT 1 cut(s) 600
Bsu36I CCTNAGG 1 cut(s) 480
BsuRI GGCC 2 cut(s) 404, 656
BsuTUI ATCGAT 1 cut(s) 600
BtgI CCRYGG 1 cut(s) 214
BtsCI GGATG 3 cut(s) 163, 214, 378
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 2 cut(s) 27, 219
Cac8I GCNNGC 3 cut(s) 99, 139, 239
CfoI GCGC 1 cut(s) 35
ClaI ATCGAT 1 cut(s) 600
Csp6I GTAC 3 cut(s) 64, 127, 571
CviAII CATG 3 cut(s) 544, 669, 690
CviQI GTAC 3 cut(s) 64, 127, 571
DdeI CTNAG 4 cut(s) 89, 480, 486, 567
DpnI GATC 3 cut(s) 233, 540, 603
DpnII GATC 3 cut(s) 231, 538, 601
Eam1104I CTCTTC 2 cut(s) 435, 689
EarI CTCTTC 2 cut(s) 435, 689
EciI GGCGGA 1 cut(s) 116
Eco130I CCWWGG 3 cut(s) 399, 556, 657
Eco147I AGGCCT 1 cut(s) 404
Eco32I GATATC 2 cut(s) 192, 364
Eco57I CTGAAG 1 cut(s) 663
Eco72I CACGTG 1 cut(s) 385
Eco81I CCTNAGG 1 cut(s) 480
EcoRII CCWGG 2 cut(s) 227, 497
EcoRV GATATC 2 cut(s) 192, 364
EcoT14I CCWWGG 3 cut(s) 399, 556, 657
ErhI CCWWGG 3 cut(s) 399, 556, 657
FaeI CATG 3 cut(s) 547, 672, 693
FaiI YATR 8 cut(s) 440, 442, 444, 534, 545, 670, 691, 730
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 1 cut(s) 134
FatI CATG 3 cut(s) 543, 668, 689
Fnu4HI GCNGC 2 cut(s) 202, 476
FokI GGATG 3 cut(s) 170, 221, 365
Fsp4HI GCNGC 2 cut(s) 202, 476
FspI TGCGCA 1 cut(s) 34
GlaI GCGC 1 cut(s) 34
GluI GCNGC 2 cut(s) 202, 476
GsaI CCCAGC 1 cut(s) 361
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 2 cut(s) 404, 656
HapII CCGG 3 cut(s) 130, 152, 195
HhaI GCGC 1 cut(s) 35
Hin1II CATG 3 cut(s) 547, 672, 693
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HindIII AAGCTT 1 cut(s) 709
HinfI GANTC 4 cut(s) 266, 369, 552, 632
HpaII CCGG 3 cut(s) 130, 152, 195
HphI GGTGA 2 cut(s) 155, 596
Hpy166II GTNNAC 1 cut(s) 573
Hpy188I TCNGA 6 cut(s) 90, 309, 368, 374, 505, 637
Hpy188III TCNNGA 5 cut(s) 152, 195, 256, 274, 455
Hpy8I GTNNAC 1 cut(s) 573
HpyAV CCTTC 1 cut(s) 646
HpyCH4IV ACGT 2 cut(s) 62, 384
HpyCH4V TGCA 2 cut(s) 237, 323
HpyF3I CTNAG 4 cut(s) 89, 480, 486, 567
HpySE526I ACGT 2 cut(s) 62, 384
Hsp92II CATG 3 cut(s) 547, 672, 693
HspAI GCGC 1 cut(s) 33
Kpn2I TCCGGA 2 cut(s) 151, 194
Kzo9I GATC 3 cut(s) 231, 538, 601
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 1 cut(s) 246
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 2 cut(s) 199, 214
MaeII ACGT 2 cut(s) 62, 384
MalI GATC 3 cut(s) 233, 540, 603
MboI GATC 3 cut(s) 231, 538, 601
MboII GAAGA 6 cut(s) 22, 387, 422, 578, 656, 706
MflI RGATCY 1 cut(s) 231
MluCI AATT 6 cut(s) 14, 260, 420, 492, 647, 725
MlyI GAGTC 1 cut(s) 626
MmeI TCCRAC 1 cut(s) 483
MroI TCCGGA 2 cut(s) 151, 194
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 2 cut(s) 491, 528
MslI CAYNNNNRTG 2 cut(s) 383, 456
MspI CCGG 3 cut(s) 130, 152, 195
MspR9I CCNGG 3 cut(s) 131, 229, 499
Mva1269I GAATGC 1 cut(s) 99
MvaI CCWGG 2 cut(s) 229, 499
NciI CCSGG 1 cut(s) 131
NdeII GATC 3 cut(s) 231, 538, 601
NlaIII CATG 3 cut(s) 547, 672, 693
NlaIV GGNNCC 1 cut(s) 242
NmeAIII GCCGAG 1 cut(s) 267
NsbI TGCGCA 1 cut(s) 34
NspI RCATGY 1 cut(s) 672
PceI AGGCCT 1 cut(s) 404
PciSI GCTCTTC 1 cut(s) 435
PctI GAATGC 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 14
PfeI GAWTC 3 cut(s) 266, 369, 552
Pfl23II CGTACG 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 262
PkrI GCNGC 2 cut(s) 203, 477
PleI GAGTC 1 cut(s) 626
PmaCI CACGTG 1 cut(s) 385
PmlI CACGTG 1 cut(s) 385
PpsI GAGTC 1 cut(s) 626
Ppu21I YACGTR 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 227, 497
PspCI CACGTG 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 357
PspGI CCWGG 2 cut(s) 227, 497
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 242
PstI CTGCAG 1 cut(s) 239
PsuI RGATCY 1 cut(s) 231
RsaI GTAC 3 cut(s) 65, 128, 572
RsaNI GTAC 3 cut(s) 64, 127, 571
RseI CAYNNNNRTG 2 cut(s) 383, 456
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 2 cut(s) 491, 528
SatI GCNGC 2 cut(s) 202, 476
Sau3AI GATC 3 cut(s) 231, 538, 601
SchI GAGTC 1 cut(s) 626
ScrFI CCNGG 3 cut(s) 131, 229, 499
SetI ASST 8 cut(s) 65, 176, 387, 430, 465, 609, 663, 713
SfaNI GCATC 2 cut(s) 199, 214
SfcI CTRYAG 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 383, 456
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse9I AATT 6 cut(s) 14, 260, 420, 492, 647, 725
SseBI AGGCCT 1 cut(s) 404
SsiI CCGC 3 cut(s) 101, 141, 476
StuI AGGCCT 1 cut(s) 404
StyD4I CCNGG 3 cut(s) 129, 227, 497
StyI CCWWGG 3 cut(s) 399, 556, 657
TaiI ACGT 2 cut(s) 65, 387
TaqI TCGA 3 cut(s) 454, 600, 618
TasI AATT 6 cut(s) 14, 260, 420, 492, 647, 725
TatI WGTACW 1 cut(s) 570
TauI GCSGC 1 cut(s) 478
TfiI GAWTC 3 cut(s) 266, 369, 552
Tru1I TTAA 2 cut(s) 491, 528
Tru9I TTAA 2 cut(s) 491, 528
TscAI CASTG 2 cut(s) 27, 226
TseI GCWGC 1 cut(s) 201
TspDTI ATGAA 1 cut(s) 459
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 2 cut(s) 27, 226
Van91I CCANNNNNTGG 1 cut(s) 262
XapI RAATTY 2 cut(s) 14, 420
XceI RCATGY 1 cut(s) 672
XmnI GAANNNNTTC 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.