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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G15020 |
| fragaria_vesca | FvH4_2g07860 |
| malus_domestica | MD00G1109200.v1.1 |
| prunus_persica | Prupe.8G137000_v2.0.a1 Prupe.8G137000_v2.0.a1 |
| pyrus_communis | pycom05g09120 |
| rosa_chinensis | RchiOBHm_Chr6g0262281 |
| rosa_laevigata | RLG00000014440 |
| rosa_multiflora | Rmu_sc0002510.1_g000031 |
| rosa_roxburghii | Rroxscaffold_7G00207970 |
| rosa_rugosa | Rorug05G0596200 |
| rosa_samantha | Rh6AG116400 Rh6BG110600 Rh6CG108000 Rh6DG098400 |
| rosa_wichuraiana | Rw6G010110 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 746 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1078 |
| AatII | GACGTC | 1 cut(s) | 1082 |
| AbsI | CCTCGAGG | 1 cut(s) | 926 |
| Acc16I | TGCGCA | 1 cut(s) | 1010 |
| Acc36I | ACCTGC | 1 cut(s) | 746 |
| AccB1I | GGYRCC | 1 cut(s) | 734 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1270 |
| AccBSI | CCGCTC | 2 cut(s) | 544, 1800 |
| AccI | GTMKAC | 3 cut(s) | 1066, 1341, 1592 |
| AccIII | TCCGGA | 1 cut(s) | 491 |
| AclWI | GGATC | 2 cut(s) | 218, 794 |
| AcoI | YGGCCR | 1 cut(s) | 1695 |
| AcsI | RAATTY | 1 cut(s) | 82 |
| AcvI | CACGTG | 4 cut(s) | 105, 1337, 1360, 1701 |
| AcyI | GRCGYC | 2 cut(s) | 1079, 1659 |
| AdeI | CACNNNGTG | 1 cut(s) | 1363 |
| AfaI | GTAC | 4 cut(s) | 899, 1022, 1250, 1811 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 757, 1163, 1270, 1454, 1495, 1584, 1633, 1782 |
| AflIII | ACRYGT | 4 cut(s) | 104, 639, 1023, 1336 |
| AgsI | TTSAA | 5 cut(s) | 187, 530, 604, 904, 1864 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 412 |
| AjiI | CACGTC | 3 cut(s) | 146, 154, 1393 |
| AjnI | CCWGG | 5 cut(s) | 756, 831, 1254, 1482, 1626 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1284, 1316 |
| AleI | CACNNNNGTG | 1 cut(s) | 572 |
| AluBI | AGCT | 8 cut(s) | 51, 112, 291, 481, 489, 754, 997, 1270 |
| AluI | AGCT | 8 cut(s) | 51, 112, 291, 481, 489, 754, 997, 1270 |
| Alw21I | GWGCWC | 1 cut(s) | 483 |
| Alw26I | GTCTC | 3 cut(s) | 68, 1227, 1925 |
| AlwI | GGATC | 2 cut(s) | 218, 794 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1075, 1270 |
| Ama87I | CYCGRG | 4 cut(s) | 476, 806, 926, 1016 |
| Aor13HI | TCCGGA | 1 cut(s) | 491 |
| ApeKI | GCWGC | 4 cut(s) | 112, 288, 910, 1267 |
| ApoI | RAATTY | 1 cut(s) | 82 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 67, 99 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1415 |
| AsuC2I | CCSGG | 2 cut(s) | 536, 709 |
| AsuHPI | GGTGA | 4 cut(s) | 967, 1339, 1375, 1924 |
| AsuII | TTCGAA | 1 cut(s) | 1548 |
| AsuNHI | GCTAGC | 1 cut(s) | 485 |
| AvaI | CYCGRG | 4 cut(s) | 476, 806, 926, 1016 |
| AvaII | GGWCC | 5 cut(s) | 4, 494, 546, 1491, 1624 |
| BalI | TGGCCA | 1 cut(s) | 1697 |
| BanI | GGYRCC | 1 cut(s) | 734 |
| BanII | GRGCYC | 1 cut(s) | 483 |
| BauI | CACGAG | 1 cut(s) | 519 |
| BbrPI | CACGTG | 4 cut(s) | 105, 1337, 1360, 1701 |
| Bbv12I | GWGCWC | 1 cut(s) | 483 |
| BbvI | GCAGC | 4 cut(s) | 99, 300, 897, 1279 |
| BccI | CCATC | 6 cut(s) | 305, 886, 1307, 1429, 1712, 1840 |
| BceAI | ACGGC | 5 cut(s) | 1347, 1596, 1646, 1744, 1828 |
| BciT130I | CCWGG | 5 cut(s) | 758, 833, 1256, 1484, 1628 |
| BciVI | GTATCC | 1 cut(s) | 1705 |
| BcnI | CCSGG | 2 cut(s) | 536, 709 |
| BcoDI | GTCTC | 3 cut(s) | 68, 1227, 1925 |
| BfaI | CTAG | 1 cut(s) | 486 |
| BfmI | CTRYAG | 1 cut(s) | 1067 |
| BfuAI | ACCTGC | 1 cut(s) | 746 |
| BfuI | GTATCC | 1 cut(s) | 1705 |
| BisI | GCNGC | 7 cut(s) | 113, 289, 733, 911, 1188, 1268, 1647 |
| BlpI | GCTNAGC | 2 cut(s) | 1183, 1796 |
| BlsI | GCNGC | 7 cut(s) | 114, 290, 734, 912, 1189, 1269, 1648 |
| Bme1390I | CCNGG | 7 cut(s) | 536, 709, 758, 833, 1256, 1484, 1628 |
| Bme18I | GGWCC | 5 cut(s) | 4, 494, 546, 1491, 1624 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 412 |
| BmeT110I | CYCGRG | 4 cut(s) | 476, 806, 926, 1016 |
| BmgBI | CACGTC | 3 cut(s) | 146, 154, 1393 |
| BmiI | GGNNCC | 8 cut(s) | 6, 267, 496, 556, 736, 918, 1563, 1625 |
| BmrFI | CCNGG | 7 cut(s) | 536, 709, 758, 833, 1256, 1484, 1628 |
| BmrI | ACTGGG | 1 cut(s) | 1909 |
| BmsI | GCATC | 2 cut(s) | 344, 979 |
| BmtI | GCTAGC | 1 cut(s) | 489 |
| BmuI | ACTGGG | 1 cut(s) | 1909 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 236, 268, 446, 478, 800, 832 |
| BpmI | CTGGAG | 3 cut(s) | 740, 1292, 1636 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1183, 1796 |
| Bpu14I | TTCGAA | 1 cut(s) | 1548 |
| BpuMI | CCSGG | 2 cut(s) | 536, 709 |
| BsaAI | YACGTR | 5 cut(s) | 105, 1337, 1360, 1468, 1701 |
| BsaHI | GRCGYC | 2 cut(s) | 1079, 1659 |
| BsaJI | CCNNGG | 8 cut(s) | 280, 654, 978, 1015, 1254, 1483, 1494, 1578 |
| BsaWI | WCCGGW | 1 cut(s) | 491 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 757, 1163, 1270, 1454, 1495, 1584, 1633, 1782 |
| Bse118I | RCCGGY | 4 cut(s) | 551, 952, 1156, 1488 |
| Bse1I | ACTGG | 2 cut(s) | 1619, 1904 |
| BseAI | TCCGGA | 1 cut(s) | 491 |
| BseBI | CCWGG | 5 cut(s) | 758, 833, 1256, 1484, 1628 |
| BseDI | CCNNGG | 8 cut(s) | 280, 654, 978, 1015, 1254, 1483, 1494, 1578 |
| BseGI | GGATG | 7 cut(s) | 335, 440, 878, 994, 1173, 1593, 1605 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 757, 1163, 1270, 1454, 1495, 1584, 1633, 1782 |
| BseMII | CTCAG | 4 cut(s) | 172, 1106, 1197, 1787 |
| BseNI | ACTGG | 2 cut(s) | 1619, 1904 |
| BseRI | GAGGAG | 5 cut(s) | 241, 244, 347, 643, 1421 |
| BseXI | GCAGC | 4 cut(s) | 99, 300, 897, 1279 |
| BseYI | CCCAGC | 1 cut(s) | 750 |
| Bsh1285I | CGRYCG | 2 cut(s) | 713, 1281 |
| BshNI | GGYRCC | 1 cut(s) | 734 |
| BsiEI | CGRYCG | 2 cut(s) | 713, 1281 |
| BsiHKAI | GWGCWC | 1 cut(s) | 483 |
| BsiHKCI | CYCGRG | 4 cut(s) | 476, 806, 926, 1016 |
| BslFI | GGGAC | 4 cut(s) | 132, 451, 1637, 1817 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 757, 1163, 1270, 1454, 1495, 1584, 1633, 1782 |
| BsmAI | GTCTC | 3 cut(s) | 68, 1227, 1925 |
| BsmBI | CGTCTC | 1 cut(s) | 1925 |
| BsmFI | GGGAC | 4 cut(s) | 132, 451, 1637, 1817 |
| BsoBI | CYCGRG | 4 cut(s) | 476, 806, 926, 1016 |
| Bsp119I | TTCGAA | 1 cut(s) | 1548 |
| Bsp1286I | GDGCHC | 1 cut(s) | 483 |
| Bsp13I | TCCGGA | 1 cut(s) | 491 |
| Bsp143I | GATC | 2 cut(s) | 223, 786 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1183, 1796 |
| Bsp19I | CCATGG | 1 cut(s) | 280 |
| BspCNI | CTCAG | 4 cut(s) | 171, 1105, 1196, 1788 |
| BspEI | TCCGGA | 1 cut(s) | 491 |
| BspLI | GGNNCC | 8 cut(s) | 6, 267, 496, 556, 736, 918, 1563, 1625 |
| BspMI | ACCTGC | 1 cut(s) | 746 |
| BspOI | GCTAGC | 1 cut(s) | 489 |
| BspPI | GGATC | 2 cut(s) | 218, 794 |
| BspT104I | TTCGAA | 1 cut(s) | 1548 |
| BspT107I | GGYRCC | 1 cut(s) | 734 |
| BsrBI | CCGCTC | 2 cut(s) | 544, 1800 |
| BsrFI | RCCGGY | 4 cut(s) | 551, 952, 1156, 1488 |
| BsrI | ACTGG | 2 cut(s) | 1619, 1904 |
| BssAI | RCCGGY | 4 cut(s) | 551, 952, 1156, 1488 |
| BssECI | CCNNGG | 8 cut(s) | 280, 654, 978, 1015, 1254, 1483, 1494, 1578 |
| BssMI | GATC | 2 cut(s) | 223, 786 |
| BssNI | GRCGYC | 2 cut(s) | 1079, 1659 |
| BssSI | CACGAG | 1 cut(s) | 519 |
| BssT1I | CCWWGG | 1 cut(s) | 280 |
| Bst2BI | CACGAG | 1 cut(s) | 519 |
| Bst2UI | CCWGG | 5 cut(s) | 758, 833, 1256, 1484, 1628 |
| Bst4CI | ACNGT | 1 cut(s) | 728 |
| Bst6I | CTCTTC | 3 cut(s) | 857, 1601, 1910 |
| BstACI | GRCGYC | 2 cut(s) | 1079, 1659 |
| BstBAI | YACGTR | 5 cut(s) | 105, 1337, 1360, 1468, 1701 |
| BstBI | TTCGAA | 1 cut(s) | 1548 |
| BstDEI | CTNAG | 6 cut(s) | 135, 158, 571, 1092, 1183, 1796 |
| BstDSI | CCRYGG | 4 cut(s) | 280, 978, 1494, 1578 |
| BstF5I | GGATG | 7 cut(s) | 335, 440, 878, 994, 1173, 1593, 1605 |
| BstKTI | GATC | 2 cut(s) | 226, 789 |
| BstMAI | GTCTC | 3 cut(s) | 68, 1227, 1925 |
| BstMBI | GATC | 2 cut(s) | 223, 786 |
| BstMCI | CGRYCG | 2 cut(s) | 713, 1281 |
| BstNI | CCWGG | 5 cut(s) | 758, 833, 1256, 1484, 1628 |
| BstNSI | RCATGY | 4 cut(s) | 177, 403, 643, 1027 |
| BstSCI | CCNGG | 7 cut(s) | 534, 707, 756, 831, 1254, 1482, 1626 |
| BstSFI | CTRYAG | 1 cut(s) | 1067 |
| BstV1I | GCAGC | 4 cut(s) | 99, 300, 897, 1279 |
| BsuI | GTATCC | 1 cut(s) | 1705 |
| BtgI | CCRYGG | 4 cut(s) | 280, 978, 1494, 1578 |
| BtrI | CACGTC | 3 cut(s) | 146, 154, 1393 |
| BtsCI | GGATG | 7 cut(s) | 335, 440, 878, 994, 1173, 1593, 1605 |
| BtsIMutI | CAGTG | 1 cut(s) | 95 |
| BveI | ACCTGC | 1 cut(s) | 746 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1075, 1270 |
| Cfr10I | RCCGGY | 4 cut(s) | 551, 952, 1156, 1488 |
| Cfr42I | CCGCGG | 1 cut(s) | 981 |
| CpoI | CGGWCCG | 1 cut(s) | 546 |
| CseI | GACGC | 3 cut(s) | 1081, 1640, 1667 |
| CsiI | ACCWGGT | 1 cut(s) | 831 |
| Csp6I | GTAC | 4 cut(s) | 898, 1021, 1249, 1810 |
| CspI | CGGWCCG | 1 cut(s) | 546 |
| CviQI | GTAC | 4 cut(s) | 898, 1021, 1249, 1810 |
| DdeI | CTNAG | 6 cut(s) | 135, 158, 571, 1092, 1183, 1796 |
| DpnI | GATC | 2 cut(s) | 225, 788 |
| DpnII | GATC | 2 cut(s) | 223, 786 |
| DraI | TTTAAA | 1 cut(s) | 316 |
| DraIII | CACNNNGTG | 1 cut(s) | 1363 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1078 |
| DriI | GACNNNNNGTC | 1 cut(s) | 412 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1078 |
| EaeI | YGGCCR | 1 cut(s) | 1695 |
| Eam1104I | CTCTTC | 3 cut(s) | 857, 1601, 1910 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 412 |
| EarI | CTCTTC | 3 cut(s) | 857, 1601, 1910 |
| Ecl136II | GAGCTC | 1 cut(s) | 481 |
| Eco130I | CCWWGG | 1 cut(s) | 280 |
| Eco147I | AGGCCT | 2 cut(s) | 248, 1124 |
| Eco24I | GRGCYC | 1 cut(s) | 483 |
| Eco47I | GGWCC | 5 cut(s) | 4, 494, 546, 1491, 1624 |
| Eco53kI | GAGCTC | 1 cut(s) | 481 |
| Eco72I | CACGTG | 4 cut(s) | 105, 1337, 1360, 1701 |
| Eco88I | CYCGRG | 4 cut(s) | 476, 806, 926, 1016 |
| EcoICRI | GAGCTC | 1 cut(s) | 481 |
| EcoO109I | RGGNCCY | 1 cut(s) | 760 |
| EcoRII | CCWGG | 5 cut(s) | 756, 831, 1254, 1482, 1626 |
| EcoT14I | CCWWGG | 1 cut(s) | 280 |
| EcoT38I | GRGCYC | 1 cut(s) | 483 |
| ErhI | CCWWGG | 1 cut(s) | 280 |
| Esp3I | CGTCTC | 1 cut(s) | 1925 |
| FaqI | GGGAC | 4 cut(s) | 132, 451, 1637, 1817 |
| FauI | CCCGC | 6 cut(s) | 750, 973, 1121, 1715, 1793, 1871 |
| FauNDI | CATATG | 1 cut(s) | 410 |
| FblI | GTMKAC | 3 cut(s) | 1066, 1341, 1592 |
| Fnu4HI | GCNGC | 7 cut(s) | 113, 289, 733, 911, 1188, 1268, 1647 |
| FokI | GGATG | 7 cut(s) | 322, 427, 865, 1001, 1180, 1600, 1612 |
| FriOI | GRGCYC | 1 cut(s) | 483 |
| Fsp4HI | GCNGC | 7 cut(s) | 113, 289, 733, 911, 1188, 1268, 1647 |
| FspAI | RTGCGCAY | 1 cut(s) | 1010 |
| FspBI | CTAG | 1 cut(s) | 486 |
| FspI | TGCGCA | 1 cut(s) | 1010 |
| GluI | GCNGC | 7 cut(s) | 113, 289, 733, 911, 1188, 1268, 1647 |
| GsaI | CCCAGC | 1 cut(s) | 754 |
| GsuI | CTGGAG | 3 cut(s) | 740, 1292, 1636 |
| HgaI | GACGC | 3 cut(s) | 1081, 1640, 1667 |
| Hin1I | GRCGYC | 2 cut(s) | 1079, 1659 |
| HincII | GTYRAC | 2 cut(s) | 1342, 1593 |
| HindII | GTYRAC | 2 cut(s) | 1342, 1593 |
| HphI | GGTGA | 4 cut(s) | 967, 1339, 1375, 1924 |
| Hpy166II | GTNNAC | 8 cut(s) | 647, 1067, 1251, 1342, 1396, 1471, 1494, 1593 |
| Hpy188I | TCNGA | 8 cut(s) | 228, 445, 460, 637, 658, 883, 1095, 1373 |
| Hpy188III | TCNNGA | 4 cut(s) | 242, 476, 492, 503 |
| Hpy8I | GTNNAC | 8 cut(s) | 647, 1067, 1251, 1342, 1396, 1471, 1494, 1593 |
| Hpy99I | CGWCG | 7 cut(s) | 1120, 1391, 1394, 1597, 1661, 1684, 1820 |
| HpyCH4III | ACNGT | 1 cut(s) | 728 |
| HpyCH4V | TGCA | 4 cut(s) | 177, 595, 942, 1041 |
| HpyF3I | CTNAG | 6 cut(s) | 135, 158, 571, 1092, 1183, 1796 |
| Hsp92I | GRCGYC | 2 cut(s) | 1079, 1659 |
| Kpn2I | TCCGGA | 1 cut(s) | 491 |
| KroI | GCCGGC | 2 cut(s) | 551, 952 |
| KroNI | GCCGGC | 2 cut(s) | 553, 954 |
| KspI | CCGCGG | 1 cut(s) | 981 |
| Kzo9I | GATC | 2 cut(s) | 223, 786 |
| LmnI | GCTCC | 7 cut(s) | 296, 494, 560, 752, 759, 922, 994 |
| Lsp1109I | GCAGC | 4 cut(s) | 99, 300, 897, 1279 |
| LweI | GCATC | 2 cut(s) | 344, 979 |
| MabI | ACCWGGT | 1 cut(s) | 831 |
| MaeI | CTAG | 1 cut(s) | 486 |
| MaeIII | GTNAC | 5 cut(s) | 574, 1148, 1327, 1670, 1895 |
| MalI | GATC | 2 cut(s) | 225, 788 |
| MbiI | CCGCTC | 2 cut(s) | 544, 1800 |
| MboI | GATC | 2 cut(s) | 223, 786 |
| MboII | GAAGA | 4 cut(s) | 817, 844, 1618, 1927 |
| MhlI | GDGCHC | 1 cut(s) | 483 |
| MlsI | TGGCCA | 1 cut(s) | 1697 |
| MluCI | AATT | 6 cut(s) | 32, 82, 182, 237, 1872, 1985 |
| MluNI | TGGCCA | 1 cut(s) | 1697 |
| MlyI | GAGTC | 6 cut(s) | 455, 464, 788, 818, 866, 1229 |
| MmeI | TCCRAC | 4 cut(s) | 582, 709, 1203, 1282 |
| Mox20I | TGGCCA | 1 cut(s) | 1697 |
| MroI | TCCGGA | 1 cut(s) | 491 |
| MroNI | GCCGGC | 2 cut(s) | 551, 952 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1415 |
| MscI | TGGCCA | 1 cut(s) | 1697 |
| MseI | TTAA | 5 cut(s) | 36, 236, 315, 1377, 1988 |
| MslI | CAYNNNNRTG | 3 cut(s) | 421, 445, 572 |
| Msp20I | TGGCCA | 1 cut(s) | 1697 |
| MspA1I | CMGCKG | 2 cut(s) | 980, 1270 |
| MspR9I | CCNGG | 7 cut(s) | 536, 709, 758, 833, 1256, 1484, 1628 |
| MvaI | CCWGG | 5 cut(s) | 758, 833, 1256, 1484, 1628 |
| NaeI | GCCGGC | 2 cut(s) | 553, 954 |
| NciI | CCSGG | 2 cut(s) | 536, 709 |
| NcoI | CCATGG | 1 cut(s) | 280 |
| NdeI | CATATG | 1 cut(s) | 410 |
| NdeII | GATC | 2 cut(s) | 223, 786 |
| NgoMIV | GCCGGC | 2 cut(s) | 551, 952 |
| NheI | GCTAGC | 1 cut(s) | 485 |
| NlaIV | GGNNCC | 8 cut(s) | 6, 267, 496, 556, 736, 918, 1563, 1625 |
| NmeAIII | GCCGAG | 2 cut(s) | 478, 565 |
| NmuCI | GTSAC | 3 cut(s) | 574, 1327, 1670 |
| NsbI | TGCGCA | 1 cut(s) | 1010 |
| NspI | RCATGY | 4 cut(s) | 177, 403, 643, 1027 |
| NspV | TTCGAA | 1 cut(s) | 1548 |
| OliI | CACNNNNGTG | 1 cut(s) | 572 |
| PaeI | GCATGC | 1 cut(s) | 177 |
| PaeR7I | CTCGAG | 2 cut(s) | 476, 926 |
| PaqCI | CACCTGC | 1 cut(s) | 746 |
| PceI | AGGCCT | 2 cut(s) | 248, 1124 |
| PciI | ACATGT | 2 cut(s) | 639, 1023 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1812 |
| PdiI | GCCGGC | 2 cut(s) | 553, 954 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1415 |
| PfeI | GAWTC | 7 cut(s) | 64, 139, 526, 1198, 1366, 1429, 1975 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1270 |
| PkrI | GCNGC | 7 cut(s) | 114, 290, 734, 912, 1189, 1269, 1648 |
| PleI | GAGTC | 6 cut(s) | 455, 463, 788, 817, 866, 1229 |
| PmaCI | CACGTG | 4 cut(s) | 105, 1337, 1360, 1701 |
| PmlI | CACGTG | 4 cut(s) | 105, 1337, 1360, 1701 |
| PpsI | GAGTC | 6 cut(s) | 455, 463, 788, 817, 866, 1229 |
| Ppu21I | YACGTR | 5 cut(s) | 105, 1337, 1360, 1468, 1701 |
| PscI | ACATGT | 2 cut(s) | 639, 1023 |
| Psp124BI | GAGCTC | 1 cut(s) | 483 |
| Psp6I | CCWGG | 5 cut(s) | 756, 831, 1254, 1482, 1626 |
| PspCI | CACGTG | 4 cut(s) | 105, 1337, 1360, 1701 |
| PspFI | CCCAGC | 1 cut(s) | 750 |
| PspGI | CCWGG | 5 cut(s) | 756, 831, 1254, 1482, 1626 |
| PspN4I | GGNNCC | 8 cut(s) | 6, 267, 496, 556, 736, 918, 1563, 1625 |
| PspXI | VCTCGAGB | 1 cut(s) | 926 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1075, 1270 |
| PvuII | CAGCTG | 1 cut(s) | 1270 |
| RsaI | GTAC | 4 cut(s) | 899, 1022, 1250, 1811 |
| RsaNI | GTAC | 4 cut(s) | 898, 1021, 1249, 1810 |
| RseI | CAYNNNNRTG | 3 cut(s) | 421, 445, 572 |
| Rsr2I | CGGWCCG | 1 cut(s) | 546 |
| RsrII | CGGWCCG | 1 cut(s) | 546 |
| SacI | GAGCTC | 1 cut(s) | 483 |
| SacII | CCGCGG | 1 cut(s) | 981 |
| SalI | GTCGAC | 2 cut(s) | 1340, 1591 |
| SaqAI | TTAA | 5 cut(s) | 36, 236, 315, 1377, 1988 |
| SatI | GCNGC | 7 cut(s) | 113, 289, 733, 911, 1188, 1268, 1647 |
| Sau3AI | GATC | 2 cut(s) | 223, 786 |
| SchI | GAGTC | 6 cut(s) | 455, 464, 788, 818, 866, 1229 |
| ScrFI | CCNGG | 7 cut(s) | 536, 709, 758, 833, 1256, 1484, 1628 |
| SduI | GDGCHC | 1 cut(s) | 483 |
| SexAI | ACCWGGT | 1 cut(s) | 831 |
| SfaNI | GCATC | 2 cut(s) | 344, 979 |
| SfcI | CTRYAG | 1 cut(s) | 1067 |
| Sfr274I | CTCGAG | 2 cut(s) | 476, 926 |
| Sfr303I | CCGCGG | 1 cut(s) | 981 |
| SfuI | TTCGAA | 1 cut(s) | 1548 |
| SgrBI | CCGCGG | 1 cut(s) | 981 |
| SinI | GGWCC | 5 cut(s) | 4, 494, 546, 1491, 1624 |
| SlaI | CTCGAG | 2 cut(s) | 476, 926 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 421, 445, 572 |
| SmlI | CTYRAG | 2 cut(s) | 476, 926 |
| SmoI | CTYRAG | 2 cut(s) | 476, 926 |
| SphI | GCATGC | 1 cut(s) | 177 |
| Sse9I | AATT | 6 cut(s) | 32, 82, 182, 237, 1872, 1985 |
| SseBI | AGGCCT | 2 cut(s) | 248, 1124 |
| SspI | AATATT | 2 cut(s) | 233, 1295 |
| SspMI | CTAG | 1 cut(s) | 486 |
| SstI | GAGCTC | 1 cut(s) | 483 |
| StuI | AGGCCT | 2 cut(s) | 248, 1124 |
| StyD4I | CCNGG | 7 cut(s) | 534, 707, 756, 831, 1254, 1482, 1626 |
| StyI | CCWWGG | 1 cut(s) | 280 |
| TaaI | ACNGT | 1 cut(s) | 728 |
| TasI | AATT | 6 cut(s) | 32, 82, 182, 237, 1872, 1985 |
| TatI | WGTACW | 2 cut(s) | 897, 1248 |
| TauI | GCSGC | 3 cut(s) | 735, 1190, 1649 |
| TfiI | GAWTC | 7 cut(s) | 64, 139, 526, 1198, 1366, 1429, 1975 |
| Tru1I | TTAA | 5 cut(s) | 36, 236, 315, 1377, 1988 |
| Tru9I | TTAA | 5 cut(s) | 36, 236, 315, 1377, 1988 |
| TscAI | CASTG | 1 cut(s) | 102 |
| TseFI | GTSAC | 3 cut(s) | 574, 1327, 1670 |
| TseI | GCWGC | 4 cut(s) | 112, 288, 910, 1267 |
| Tsp45I | GTSAC | 3 cut(s) | 574, 1327, 1670 |
| TspDTI | ATGAA | 7 cut(s) | 231, 829, 994, 1184, 1550, 1586, 1704 |
| TspGWI | ACGGA | 4 cut(s) | 574, 1133, 1511, 1833 |
| TspRI | CASTG | 1 cut(s) | 102 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1270 |
| VpaK11BI | GGWCC | 5 cut(s) | 4, 494, 546, 1491, 1624 |
| XapI | RAATTY | 1 cut(s) | 82 |
| XceI | RCATGY | 4 cut(s) | 177, 403, 643, 1027 |
| XhoI | CTCGAG | 2 cut(s) | 476, 926 |
| XmiI | GTMKAC | 3 cut(s) | 1066, 1341, 1592 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1415 |
| XspI | CTAG | 1 cut(s) | 486 |
| ZraI | GACGTC | 1 cut(s) | 1080 |