pycom05g11540

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0017042
Physical Location & Seq
Forward (+)
15145849 .. 15147045
1197 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 447 bp
ATGGCCTCAAAGAGACTATTGAAGGAGCTCAAGGACTTGCAAAGAGATCCACCAACTTCATGTAGTGCAGGTCCTGTGGCTGAGGACATGTTTCATTGGCAAGCAACAATAATTGGTCCAAATGATAGCCCTTATTCTGGGGGTGTCTTCCTTGTGACTATCCATTTTCCACCTGATTATCCTTTCAAACCTCCAAAGGTTGCCTTCAGAACTAAAGTGTTCCATCCAAATATAAACAGCAGCGGCAGTATCTGCTTGGACATTCTCAAGGAGCAATGGAGTCCAGCACTCACCATTTCCAAGGTTTTACTGTCCATATGTTCATTGCTAACCGATCCAAACCCTGATGACCCTCTTGTGCCTGAGATTGCTCATATGTGCAAGAGTGACAAAGTCAAATATGAGTCAACGGCTCGGAGTTGGACCCAGAAGTATGCCAATTCTTAA

Protein Analysis

149

Amino Acids

16.47

Weight (kDa)

7.68

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 2.8e-53 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017042)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16740
fragaria_vesca FvH4_2g01240
malus_domestica MD05G1121200.v1.1
prunus_persica Prupe.8G165100_v2.0.a1
pyrus_communis pycom05g11540 pycom10g10670
rosa_chinensis RchiOBHm_Chr6g0251901
rosa_laevigata RLG00000015421
rosa_multiflora Rmu_ssc0000303.1_g000012
rosa_roxburghii Rroxscaffold_7G00216320
rosa_rugosa Rorug05G0504900
rosa_samantha Rh6BG014100
rosa_wichuraiana Rw6G001540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 59
AciI CCGC 1 cut(s) 243
AclWI GGATC 2 cut(s) 41, 329
AcuI CTGAAG 1 cut(s) 190
AfiI CCNNNNNNNGG 1 cut(s) 137
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 2 cut(s) 22, 187
AluBI AGCT 1 cut(s) 28
AluI AGCT 1 cut(s) 28
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 7
AlwI GGATC 2 cut(s) 41, 329
AlwNI CAGNNNCTG 2 cut(s) 74, 252
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 240
AspS9I GGNCC 3 cut(s) 71, 116, 423
AsuHPI GGTGA 1 cut(s) 283
AvaII GGWCC 3 cut(s) 71, 116, 423
BanII GRGCYC 1 cut(s) 30
BbsI GAAGAC 1 cut(s) 139
Bbv12I GWGCWC 1 cut(s) 30
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 252
BccI CCATC 1 cut(s) 231
BceAI ACGGC 1 cut(s) 426
BcoDI GTCTC 1 cut(s) 7
BfuAI ACCTGC 1 cut(s) 59
BisI GCNGC 2 cut(s) 241, 244
BlsI GCNGC 2 cut(s) 242, 245
Bme18I GGWCC 3 cut(s) 71, 116, 423
BmgT120I GGNCC 3 cut(s) 71, 116, 423
BmiI GGNNCC 1 cut(s) 425
BpiI GAAGAC 1 cut(s) 139
Bpu10I CCTNAGC 1 cut(s) 81
BpuEI CTTGAG 2 cut(s) 14, 251
BsaJI CCNNGG 1 cut(s) 300
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse3DI GCAATG 2 cut(s) 281, 323
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMI GCAATG 2 cut(s) 281, 323
BseMII CTCAG 2 cut(s) 72, 354
BseXI GCAGC 1 cut(s) 252
BsgI GTGCAG 1 cut(s) 87
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 137
BsmAI GTCTC 1 cut(s) 7
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 2 cut(s) 46, 334
BspACI CCGC 1 cut(s) 243
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 73, 355
BspLI GGNNCC 1 cut(s) 425
BspMI ACCTGC 1 cut(s) 59
BspPI GGATC 2 cut(s) 41, 329
BsrDI GCAATG 2 cut(s) 281, 323
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 2 cut(s) 46, 334
BssT1I CCWWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 312
BstAPI GCANNNNNTGC 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 2 cut(s) 81, 363
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 2 cut(s) 49, 337
BstMAI GTCTC 1 cut(s) 7
BstMBI GATC 2 cut(s) 46, 334
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstNSI RCATGY 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 139
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 223
BveI ACCTGC 1 cut(s) 59
Cac8I GCNNGC 1 cut(s) 102
CaiI CAGNNNCTG 2 cut(s) 74, 252
Cfr13I GGNCC 3 cut(s) 71, 116, 423
CviAII CATG 2 cut(s) 60, 88
CviJI RGCY 5 cut(s) 5, 28, 80, 129, 413
CviKI_1 RGCY 5 cut(s) 5, 28, 80, 129, 413
DdeI CTNAG 2 cut(s) 81, 363
DpnI GATC 2 cut(s) 48, 336
DpnII GATC 2 cut(s) 46, 334
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 30
Eco47I GGWCC 3 cut(s) 71, 116, 423
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 190
EcoICRI GAGCTC 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 2 cut(s) 63, 91
FaiI YATR 9 cut(s) 61, 89, 233, 317, 319, 375, 377, 402, 435
FalI AAGNNNNNCTT 2 cut(s) 188, 220
FatI CATG 2 cut(s) 59, 87
FauNDI CATATG 2 cut(s) 317, 375
Fnu4HI GCNGC 2 cut(s) 241, 244
FokI GGATG 1 cut(s) 210
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 2 cut(s) 241, 244
GluI GCNGC 2 cut(s) 241, 244
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 2 cut(s) 63, 91
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 2 cut(s) 280, 404
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 2 cut(s) 209, 417
Hpy8I GTNNAC 1 cut(s) 408
HpyAV CCTTC 2 cut(s) 16, 214
HpyCH4III ACNGT 1 cut(s) 312
HpyCH4V TGCA 3 cut(s) 40, 68, 381
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
HpyF3I CTNAG 2 cut(s) 81, 363
Hsp92II CATG 2 cut(s) 63, 91
Kzo9I GATC 2 cut(s) 46, 334
LmnI GCTCC 2 cut(s) 25, 271
LpnPI CCDG 8 cut(s) 54, 87, 123, 186, 297, 357, 375, 440
Lsp1109I GCAGC 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 154, 386
MalI GATC 2 cut(s) 48, 336
MboI GATC 2 cut(s) 46, 334
MboII GAAGA 1 cut(s) 139
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 2 cut(s) 111, 439
MlyI GAGTC 2 cut(s) 289, 413
MmeI TCCRAC 1 cut(s) 401
MnlI CCTC 4 cut(s) 16, 76, 201, 363
MseI TTAA 1 cut(s) 445
MspA1I CMGCKG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 252
NdeI CATATG 2 cut(s) 317, 375
NdeII GATC 2 cut(s) 46, 334
NlaIII CATG 2 cut(s) 63, 91
NlaIV GGNNCC 1 cut(s) 425
NmuCI GTSAC 2 cut(s) 154, 386
NspI RCATGY 1 cut(s) 91
PciI ACATGT 1 cut(s) 87
PflFI GACNNNGTC 1 cut(s) 392
PkrI GCNGC 2 cut(s) 242, 245
PleI GAGTC 2 cut(s) 288, 412
PpsI GAGTC 2 cut(s) 288, 412
PpuMI RGGWCCY 1 cut(s) 71
PscI ACATGT 1 cut(s) 87
Psp124BI GAGCTC 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 425
PspPI GGNCC 3 cut(s) 71, 116, 423
PspPPI RGGWCCY 1 cut(s) 71
PstNI CAGNNNCTG 2 cut(s) 74, 252
PsuI RGATCY 1 cut(s) 46
PsyI GACNNNGTC 1 cut(s) 392
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 1 cut(s) 445
SatI GCNGC 2 cut(s) 241, 244
Sau3AI GATC 2 cut(s) 46, 334
Sau96I GGNCC 3 cut(s) 71, 116, 423
SchI GAGTC 2 cut(s) 289, 413
SduI GDGCHC 1 cut(s) 30
SetI ASST 6 cut(s) 30, 73, 175, 193, 201, 306
SinI GGWCC 3 cut(s) 71, 116, 423
SmlI CTYRAG 2 cut(s) 29, 266
SmoI CTYRAG 2 cut(s) 29, 266
Sse9I AATT 2 cut(s) 111, 439
SsiI CCGC 1 cut(s) 243
SstI GAGCTC 1 cut(s) 30
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 312
TasI AATT 2 cut(s) 111, 439
TauI GCSGC 1 cut(s) 246
Tru1I TTAA 1 cut(s) 445
Tru9I TTAA 1 cut(s) 445
TseFI GTSAC 2 cut(s) 154, 386
TseI GCWGC 1 cut(s) 240
Tsp45I GTSAC 2 cut(s) 154, 386
TspDTI ATGAA 3 cut(s) 48, 83, 312
Tth111I GACNNNGTC 1 cut(s) 392
VpaK11BI GGWCC 3 cut(s) 71, 116, 423
XceI RCATGY 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.