pycom05g11580

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013867
Physical Location & Seq
Reverse (-)
15180231 .. 15181139
909 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 465 bp
ATGGGTGTTTTGGATCATTTTTCTGATCTATTTGATTGCTCAAGTGGGAGTTCCAGGCTCAAGAAACGCAGACAATTCCAGACGGTGGAGATCAAGGTGAAGATGGACTGCGAAGGATGCGAGCGGAAGGTGAAGAGATCGGTGGAAGGAATGAAGGGCGTAACGCAGGTGGACGTGGACCGCAAGGCCCACAAGCTGACGGTCGTCGGATACGTGGATCCCGGCAAGGTGCTGGCGCGCGTCGCGCATCGGACGGGGAAGAAGGTGGAGTTCTGGCCATACGTCCCCTACGACGTCGTGGCGCACCCTTACGCCGCTGGAGTGTACGACAAGAAGGCGCCGTCCGGGTACGTGCGGAACGCGCAGGACCCGCAGATGAGGAACCTCGCACGTGCCAGCTCCACGGAAGTGCGCTACACCACGGCATTCAGCGATGAAAATCCCGATGCGTGCGTTGTGATGTGA

Protein Analysis

155

Amino Acids

17.29

Weight (kDa)

9.28

Isoelectric Point (pI)

29.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 32 - 87 1.4e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013867)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38580 AT4G38580
fragaria_vesca FvH4_2g01160
malus_domestica MD05G1121800.v1.1 MD10G1124400.v1.1
prunus_persica Prupe.8G165600_v2.0.a1
pyrus_communis pycom05g11580
rosa_chinensis RchiOBHm_Chr6g0251781
rosa_laevigata RLG00000015430
rosa_multiflora Rmu_ssc0000213.1_g000113
rosa_roxburghii Rroxscaffold_7G00216390
rosa_rugosa Rorug05G0503900
rosa_samantha Rh6AG014700 Rh6BG013100 Rh6CG010100 Rh6DG011300
rosa_wichuraiana Rw6G001460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 157
AatII GACGTC 1 cut(s) 297
Acc36I ACCTGC 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 337
AccB7I CCANNNNNTGG 1 cut(s) 85
AccBSI CCGCTC 1 cut(s) 124
AccII CGCG 4 cut(s) 238, 240, 245, 362
AciI CCGC 5 cut(s) 124, 181, 315, 355, 371
AclWI GGATC 3 cut(s) 21, 212, 225
AcoI YGGCCR 1 cut(s) 275
AcvI CACGTG 1 cut(s) 392
AcyI GRCGYC 2 cut(s) 294, 338
AfaI GTAC 2 cut(s) 326, 350
AfiI CCNNNNNNNGG 1 cut(s) 85
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 1 cut(s) 53
AleI CACNNNNGTG 1 cut(s) 407
AluBI AGCT 2 cut(s) 196, 399
AluI AGCT 2 cut(s) 196, 399
AlwI GGATC 3 cut(s) 21, 212, 225
AoxI GGCC 2 cut(s) 186, 275
AspLEI GCGC 7 cut(s) 238, 240, 247, 304, 340, 364, 414
AspS9I GGNCC 3 cut(s) 178, 187, 367
AsuC2I CCSGG 2 cut(s) 222, 346
AsuHPI GGTGA 2 cut(s) 109, 142
AvaII GGWCC 2 cut(s) 178, 367
BalI TGGCCA 1 cut(s) 277
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 337
BbrPI CACGTG 1 cut(s) 392
BccI CCATC 1 cut(s) 97
BceAI ACGGC 2 cut(s) 325, 438
BciT130I CCWGG 1 cut(s) 55
BciVI GTATCC 1 cut(s) 203
BcnI CCSGG 2 cut(s) 222, 346
BfoI RGCGCY 1 cut(s) 341
BfuAI ACCTGC 1 cut(s) 157
BfuI GTATCC 1 cut(s) 203
BisI GCNGC 1 cut(s) 315
BlsI GCNGC 1 cut(s) 316
Bme1390I CCNGG 3 cut(s) 55, 222, 346
Bme18I GGWCC 2 cut(s) 178, 367
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 3 cut(s) 178, 187, 367
BmiI GGNNCC 4 cut(s) 219, 339, 369, 383
BmrFI CCNGG 3 cut(s) 55, 222, 346
BmsI GCATC 3 cut(s) 107, 256, 436
BoxI GACNNNNGTC 1 cut(s) 203
BpmI CTGGAG 1 cut(s) 339
BpuEI CTTGAG 2 cut(s) 25, 44
BpuMI CCSGG 2 cut(s) 222, 346
BsaAI YACGTR 3 cut(s) 214, 352, 392
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 2 cut(s) 294, 338
BsaJI CCNNGG 2 cut(s) 402, 420
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 1 cut(s) 55
BseDI CCNNGG 2 cut(s) 402, 420
BseGI GGATG 1 cut(s) 122
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 1 cut(s) 85
BsePI GCGCGC 1 cut(s) 236
Bsh1236I CGCG 4 cut(s) 238, 240, 245, 362
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 188, 277
BshNI GGYRCC 1 cut(s) 337
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 2 cut(s) 222, 345
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 1 cut(s) 85
BsmFI GGGAC 1 cut(s) 269
BsmI GAATGC 1 cut(s) 425
BsnI GGCC 2 cut(s) 188, 277
Bsp143I GATC 5 cut(s) 13, 25, 90, 137, 217
BspACI CCGC 5 cut(s) 124, 181, 315, 355, 371
BspANI GGCC 2 cut(s) 188, 277
BspFNI CGCG 4 cut(s) 238, 240, 245, 362
BspLI GGNNCC 4 cut(s) 219, 339, 369, 383
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 3 cut(s) 21, 212, 225
BspT107I GGYRCC 1 cut(s) 337
BsrBI CCGCTC 1 cut(s) 124
BssECI CCNNGG 2 cut(s) 402, 420
BssHII GCGCGC 1 cut(s) 236
BssMI GATC 5 cut(s) 13, 25, 90, 137, 217
BssNI GRCGYC 2 cut(s) 294, 338
Bst2UI CCWGG 1 cut(s) 55
Bst4CI ACNGT 2 cut(s) 85, 202
Bst6I CTCTTC 1 cut(s) 128
BstACI GRCGYC 2 cut(s) 294, 338
BstBAI YACGTR 3 cut(s) 214, 352, 392
BstC8I GCNNGC 5 cut(s) 122, 234, 238, 397, 451
BstDSI CCRYGG 2 cut(s) 402, 420
BstF5I GGATG 1 cut(s) 122
BstFNI CGCG 4 cut(s) 238, 240, 245, 362
BstH2I RGCGCY 1 cut(s) 341
BstHHI GCGC 7 cut(s) 238, 240, 247, 304, 340, 364, 414
BstKTI GATC 5 cut(s) 16, 28, 93, 140, 220
BstMBI GATC 5 cut(s) 13, 25, 90, 137, 217
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 5 cut(s) 117, 242, 244, 361, 370
BstNI CCWGG 1 cut(s) 55
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 3 cut(s) 53, 220, 344
BstUI CGCG 4 cut(s) 238, 240, 245, 362
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuI GTATCC 1 cut(s) 203
BsuRI GGCC 2 cut(s) 188, 277
BtgI CCRYGG 2 cut(s) 402, 420
BtgZI GCGATG 1 cut(s) 447
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 1 cut(s) 122
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 5 cut(s) 122, 234, 238, 397, 451
CfoI GCGC 7 cut(s) 238, 240, 247, 304, 340, 364, 414
Cfr13I GGNCC 3 cut(s) 178, 187, 367
CseI GACGC 1 cut(s) 229
Csp6I GTAC 2 cut(s) 325, 349
CviJI RGCY 5 cut(s) 58, 188, 196, 277, 399
CviKI_1 RGCY 5 cut(s) 58, 188, 196, 277, 399
CviQI GTAC 2 cut(s) 325, 349
DinI GGCGCC 1 cut(s) 339
DpnI GATC 5 cut(s) 15, 27, 92, 139, 219
DpnII GATC 5 cut(s) 13, 25, 90, 137, 217
EaeI YGGCCR 1 cut(s) 275
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco47I GGWCC 2 cut(s) 178, 367
Eco72I CACGTG 1 cut(s) 392
EcoO109I RGGNCCY 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 53
EgeI GGCGCC 1 cut(s) 339
EheI GGCGCC 1 cut(s) 339
FaiI YATR 1 cut(s) 280
FaqI GGGAC 1 cut(s) 269
FauI CCCGC 1 cut(s) 378
Fnu4HI GCNGC 1 cut(s) 315
FokI GGATG 1 cut(s) 129
Fsp4HI GCNGC 1 cut(s) 315
GlaI GCGC 7 cut(s) 237, 239, 246, 303, 339, 363, 413
GluI GCNGC 1 cut(s) 315
GsuI CTGGAG 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 341
HaeIII GGCC 2 cut(s) 188, 277
HapII CCGG 2 cut(s) 222, 345
HgaI GACGC 1 cut(s) 229
HhaI GCGC 7 cut(s) 238, 240, 247, 304, 340, 364, 414
Hin1I GRCGYC 2 cut(s) 294, 338
Hin6I GCGC 7 cut(s) 236, 238, 245, 302, 338, 362, 412
HinP1I GCGC 7 cut(s) 236, 238, 245, 302, 338, 362, 412
HpaII CCGG 2 cut(s) 222, 345
HphI GGTGA 2 cut(s) 109, 142
Hpy166II GTNNAC 3 cut(s) 172, 178, 325
Hpy188I TCNGA 3 cut(s) 25, 209, 252
Hpy188III TCNNGA 3 cut(s) 61, 79, 443
Hpy8I GTNNAC 3 cut(s) 172, 178, 325
Hpy99I CGWCG 4 cut(s) 209, 245, 296, 299
HpyAV CCTTC 6 cut(s) 107, 121, 140, 148, 256, 328
HpyCH4III ACNGT 2 cut(s) 85, 202
HpyCH4IV ACGT 6 cut(s) 174, 213, 282, 294, 351, 391
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 242, 244, 361, 370
HpySE526I ACGT 6 cut(s) 174, 213, 282, 294, 351, 391
Hsp92I GRCGYC 2 cut(s) 294, 338
HspAI GCGC 7 cut(s) 236, 238, 245, 302, 338, 362, 412
KasI GGCGCC 1 cut(s) 337
Kzo9I GATC 5 cut(s) 13, 25, 90, 137, 217
LmnI GCTCC 1 cut(s) 404
LweI GCATC 3 cut(s) 107, 256, 436
MaeII ACGT 6 cut(s) 174, 213, 282, 294, 351, 391
MaeIII GTNAC 1 cut(s) 160
MalI GATC 5 cut(s) 15, 27, 92, 139, 219
MbiI CCGCTC 1 cut(s) 124
MboI GATC 5 cut(s) 13, 25, 90, 137, 217
MboII GAAGA 3 cut(s) 112, 145, 271
MflI RGATCY 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 277
MluCI AATT 1 cut(s) 74
MluNI TGGCCA 1 cut(s) 277
Mly113I GGCGCC 1 cut(s) 338
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 2 cut(s) 372, 395
Mox20I TGGCCA 1 cut(s) 277
MscI TGGCCA 1 cut(s) 277
MslI CAYNNNNRTG 1 cut(s) 407
Msp20I TGGCCA 1 cut(s) 277
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 2 cut(s) 222, 345
MspR9I CCNGG 3 cut(s) 55, 222, 346
Mva1269I GAATGC 1 cut(s) 425
MvaI CCWGG 1 cut(s) 55
MvnI CGCG 4 cut(s) 238, 240, 245, 362
MwoI GCNNNNNNNGC 5 cut(s) 117, 242, 244, 361, 370
NarI GGCGCC 1 cut(s) 338
NciI CCSGG 2 cut(s) 222, 346
NdeII GATC 5 cut(s) 13, 25, 90, 137, 217
NlaIV GGNNCC 4 cut(s) 219, 339, 369, 383
OliI CACNNNNGTG 1 cut(s) 407
PaqCI CACCTGC 1 cut(s) 157
PauI GCGCGC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 288
PctI GAATGC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 85
PkrI GCNGC 1 cut(s) 316
PluTI GGCGCC 1 cut(s) 341
PmaCI CACGTG 1 cut(s) 392
PmlI CACGTG 1 cut(s) 392
Ppu21I YACGTR 3 cut(s) 214, 352, 392
PpuMI RGGWCCY 1 cut(s) 367
PshAI GACNNNNGTC 1 cut(s) 203
Psp5II RGGWCCY 1 cut(s) 367
Psp6I CCWGG 1 cut(s) 53
PspCI CACGTG 1 cut(s) 392
PspGI CCWGG 1 cut(s) 53
PspN4I GGNNCC 4 cut(s) 219, 339, 369, 383
PspPI GGNCC 3 cut(s) 178, 187, 367
PspPPI RGGWCCY 1 cut(s) 367
PsuI RGATCY 1 cut(s) 217
PteI GCGCGC 1 cut(s) 236
RsaI GTAC 2 cut(s) 326, 350
RsaNI GTAC 2 cut(s) 325, 349
RseI CAYNNNNRTG 1 cut(s) 407
SatI GCNGC 1 cut(s) 315
Sau3AI GATC 5 cut(s) 13, 25, 90, 137, 217
Sau96I GGNCC 3 cut(s) 178, 187, 367
ScrFI CCNGG 3 cut(s) 55, 222, 346
SfaNI GCATC 3 cut(s) 107, 256, 436
SfoI GGCGCC 1 cut(s) 339
SinI GGWCC 2 cut(s) 178, 367
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 2 cut(s) 40, 59
SmoI CTYRAG 2 cut(s) 40, 59
Sse9I AATT 1 cut(s) 74
SsiI CCGC 5 cut(s) 124, 181, 315, 355, 371
SspDI GGCGCC 1 cut(s) 337
StyD4I CCNGG 3 cut(s) 53, 220, 344
TaaI ACNGT 2 cut(s) 85, 202
TaiI ACGT 6 cut(s) 177, 216, 285, 297, 354, 394
TasI AATT 1 cut(s) 74
TauI GCSGC 1 cut(s) 317
TspDTI ATGAA 2 cut(s) 167, 450
TspGWI ACGGA 1 cut(s) 419
Van91I CCANNNNNTGG 1 cut(s) 85
VpaK11BI GGWCC 2 cut(s) 178, 367
ZraI GACGTC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.