pycom05g18340

VHS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011482
Physical Location & Seq
Forward (+)
21314134 .. 21316834
2701 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18340.3

Sequence Viewer

Length: 1305 bp
ATGGACAAGTTGAATCTATCTCAGTTGGGGGAGAGGTTAAAGATAGGTGGAGCTCAAATGGGTCGAATGGTAAGTGGGAAAATGAAGGAAATGAAGGAGATTCTGCAAGCACCGACCCCGGAATCAAAGATGATCGACGAGGCCACTCTGGAGACCATGGAGGAGCCGAATTGGGGTATGAATTTGAGGATATGTGCAATGATCAATAGTGAAGAGTTTAGTGGGTCCGAAGTTGTTAAAGCCATAAAGAAGAAGATTTCGGGTAAGAGTGTGGTGAGCCAGAGGCTGAGTCTTGACTTGTTGGAGGCTTGTACTATGAATTGCGAAAAGGTGTTTTCGGAGGTTGCATCGGAGAAGGTGTTGGAGGAGATGCTTAGGATGATCGATAATCCGCAAACGGATCCTGAAAATAGACGAAGGGCTGTGCAATTGATTAGGGCTTGGGGAGAGTCTGAGGATCTTGCGTATCTGCCTGTATTTCATCAAACTTATGTGAGCTTGAGAGAAAGAAGCACCGATCCTTTAGCACAAGAAGGGGCTTCGCCCTCGTTGCAGTCTACCTTGGAGTCATATGTTCATGACCCGCTGTCTCCCCCTGAAAGATACCCTGTACCTGACACAGGATTGCATGGAGGAGCAAATCATAGTGACTTCCCCTTTAACTATCAAAACATAGCAGTTGAAGACAAAAAAGAACTTCTTGTCGTAGCTCGTAATAGCCTTGAACTTCTGTCAAGTATTTTGAATACCGAAACAGATCCGAAACCTTTGAAGGAAGAACTAACCGTAAACATGCTGGACAAGTGCAAGGAGTCTCAGCCAGTTATTAAAAGGATCATAGAAACCACTACCGATGATGAAGGGATGCTCTTTGAGGCCCTATTCCTTCACGAAGAACTCCAACAGATCATTTCCAAGTACGAAGAATTGGAAGCTTCTCAAAAATCTGGAATCCAACAGCTTGAAAATCCTGCAATCACCTGGGATGAGGGGATGGAAGCTGGTCTAAACCCTGGAGAACTCCCTGGAAACTCCAACACCACTGAGCGCGAGGATTTGGAAGACACCCAAAAACATGATGGAAAACTGCCCGAAAATTCCAGTGCTAATGAAGGCAGCTCGTCCGCTCAGGTAGGAAACCATAGCGAAACCAAAATAGTTGGCTCTTCGCAGCAAGGCACCAAATCCAGCGGTGCTTCTGAAGGCGGCTCATCCGCTGAGGTAGGAGGAAAACATAGCGAAACCAAAATAGTTGATTCTCCGCACAAAGACCCCAAGTCCAGCAGCGAAAAATTAGGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0032182 GO:0043130 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GAT VHS

Protein Analysis

435

Amino Acids

47.84

Weight (kDa)

4.79

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06210 AT1G06210
fragaria_vesca FvH4_2g21370 FvH4_2g21370
malus_domestica MD05G1196400.v1.1
prunus_persica Prupe.8G222800_v2.0.a1
pyrus_communis pycom05g18340
rosa_chinensis RchiOBHm_Chr6g0287961
rosa_laevigata RLG00000012451
rosa_multiflora Rmu_sc0005266.1_g000005 Rmu_sc0005309.1_g000002
rosa_roxburghii Rroxscaffold_7G00180630 Rroxscaffold_7G00180910
rosa_rugosa Rorug06G0193700
rosa_samantha Rh6AG303000 Rh6BG309200 Rh6CG300500 Rh6CG316900 Rh6DG301700
rosa_wichuraiana Rw6G026200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1178
AccBSI CCGCTC 1 cut(s) 1127
AccI GTMKAC 1 cut(s) 557
AccII CGCG 1 cut(s) 1050
AciI CCGC 7 cut(s) 392, 584, 1125, 1191, 1206, 1215, 1262
AclWI GGATC 6 cut(s) 395, 408, 465, 512, 752, 842
AcsI RAATTY 2 cut(s) 181, 1096
AcuI CTGAAG 1 cut(s) 1221
AfaI GTAC 3 cut(s) 313, 612, 920
AfiI CCNNNNNNNGG 2 cut(s) 173, 620
AgsI TTSAA 6 cut(s) 13, 683, 725, 745, 772, 965
AhdI GACNNNNNGTC 2 cut(s) 586, 1276
AjnI CCWGG 3 cut(s) 980, 1012, 1024
AluBI AGCT 7 cut(s) 53, 498, 710, 935, 961, 1001, 1119
AluI AGCT 7 cut(s) 53, 498, 710, 935, 961, 1001, 1119
Alw21I GWGCWC 1 cut(s) 55
Alw26I GTCTC 3 cut(s) 146, 594, 819
AlwI GGATC 6 cut(s) 395, 408, 465, 512, 752, 842
AlwNI CAGNNNCTG 1 cut(s) 286
AoxI GGCC 2 cut(s) 141, 876
ApeKI GCWGC 3 cut(s) 1116, 1171, 1284
ApoI RAATTY 2 cut(s) 181, 1096
AspLEI GCGC 1 cut(s) 1050
AspS9I GGNCC 2 cut(s) 225, 877
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 286, 970
AvaII GGWCC 1 cut(s) 225
BamHI GGATCC 1 cut(s) 400
BanI GGYRCC 1 cut(s) 1178
BanII GRGCYC 1 cut(s) 55
BbsI GAAGAC 2 cut(s) 690, 1068
Bbv12I GWGCWC 1 cut(s) 55
BbvCI CCTCAGC 1 cut(s) 1218
BbvI GCAGC 3 cut(s) 1128, 1183, 1296
BccI CCATC 2 cut(s) 988, 1073
BciT130I CCWGG 3 cut(s) 982, 1014, 1026
BclI TGATCA 1 cut(s) 201
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 3 cut(s) 146, 594, 819
BisI GCNGC 4 cut(s) 1117, 1172, 1207, 1285
BlsI GCNGC 4 cut(s) 1118, 1173, 1208, 1286
Bme1390I CCNGG 4 cut(s) 119, 982, 1014, 1026
Bme18I GGWCC 1 cut(s) 225
BmeRI GACNNNNNGTC 2 cut(s) 586, 1276
BmgT120I GGNCC 2 cut(s) 225, 877
BmiI GGNNCC 4 cut(s) 165, 226, 402, 1180
BmrFI CCNGG 4 cut(s) 119, 982, 1014, 1026
BmsI GCATC 3 cut(s) 356, 360, 855
BpiI GAAGAC 2 cut(s) 690, 1068
BpmI CTGGAG 2 cut(s) 170, 1035
Bpu10I CCTNAGC 3 cut(s) 374, 1128, 1218
BpuEI CTTGAG 1 cut(s) 520
BpuMI CCSGG 1 cut(s) 119
Bsa29I ATCGAT 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 6 cut(s) 117, 156, 561, 981, 1012, 1024
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 620
Bse1I ACTGG 2 cut(s) 821, 1101
Bse3DI GCAATG 1 cut(s) 204
BseBI CCWGG 3 cut(s) 982, 1014, 1026
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 6 cut(s) 117, 156, 561, 981, 1012, 1024
BseGI GGATG 5 cut(s) 384, 870, 991, 999, 1211
BseLI CCNNNNNNNGG 2 cut(s) 173, 620
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 7 cut(s) 35, 278, 444, 830, 1035, 1142, 1209
BseNI ACTGG 2 cut(s) 821, 1101
BseRI GAGGAG 3 cut(s) 176, 380, 648
BseXI GCAGC 3 cut(s) 1128, 1183, 1296
Bsh1236I CGCG 1 cut(s) 1050
BshFI GGCC 2 cut(s) 143, 878
BshNI GGYRCC 1 cut(s) 1178
BshVI ATCGAT 1 cut(s) 384
BsiHKAI GWGCWC 1 cut(s) 55
BsiSI CCGG 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 173, 620
BsmAI GTCTC 3 cut(s) 146, 594, 819
BsnI GGCC 2 cut(s) 143, 878
Bso31I GGTCTC 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 55
Bsp143I GATC 9 cut(s) 132, 201, 381, 400, 457, 517, 757, 834, 906
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 7 cut(s) 392, 584, 1125, 1191, 1206, 1215, 1262
BspANI GGCC 2 cut(s) 143, 878
BspCNI CTCAG 7 cut(s) 34, 279, 445, 829, 1036, 1141, 1210
BspDI ATCGAT 1 cut(s) 384
BspFNI CGCG 1 cut(s) 1050
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 4 cut(s) 165, 226, 402, 1180
BspPI GGATC 6 cut(s) 395, 408, 465, 512, 752, 842
BspQI GCTCTTC 1 cut(s) 1171
BspT107I GGYRCC 1 cut(s) 1178
BspTNI GGTCTC 1 cut(s) 146
BsrBI CCGCTC 1 cut(s) 1127
BsrDI GCAATG 1 cut(s) 204
BsrI ACTGG 2 cut(s) 821, 1101
BssECI CCNNGG 6 cut(s) 117, 156, 561, 981, 1012, 1024
BssMI GATC 9 cut(s) 132, 201, 381, 400, 457, 517, 757, 834, 906
BssT1I CCWWGG 2 cut(s) 156, 561
Bst2UI CCWGG 3 cut(s) 982, 1014, 1026
Bst4CI ACNGT 1 cut(s) 787
Bst6I CTCTTC 2 cut(s) 207, 1171
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 8 cut(s) 21, 287, 374, 453, 816, 1044, 1128, 1218
BstDSI CCRYGG 1 cut(s) 156
BstENI CCTNNNNNAGG 1 cut(s) 618
BstF5I GGATG 5 cut(s) 384, 870, 991, 999, 1211
BstFNI CGCG 1 cut(s) 1050
BstHHI GCGC 1 cut(s) 1050
BstKTI GATC 9 cut(s) 135, 204, 384, 403, 460, 520, 760, 837, 909
BstMAI GTCTC 3 cut(s) 146, 594, 819
BstMBI GATC 9 cut(s) 132, 201, 381, 400, 457, 517, 757, 834, 906
BstMWI GCNNNNNNNGC 1 cut(s) 550
BstNI CCWGG 3 cut(s) 982, 1014, 1026
BstNSI RCATGY 1 cut(s) 796
BstSCI CCNGG 4 cut(s) 117, 980, 1012, 1024
BstUI CGCG 1 cut(s) 1050
BstV1I GCAGC 3 cut(s) 1128, 1183, 1296
BstV2I GAAGAC 2 cut(s) 690, 1068
BstX2I RGATCY 3 cut(s) 400, 457, 757
BstYI RGATCY 3 cut(s) 400, 457, 757
Bsu15I ATCGAT 1 cut(s) 384
BsuRI GGCC 2 cut(s) 143, 878
BsuTUI ATCGAT 1 cut(s) 384
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 5 cut(s) 384, 870, 991, 999, 1211
BtsIMutI CAGTG 2 cut(s) 1041, 1108
Cac8I GCNNGC 1 cut(s) 108
CaiI CAGNNNCTG 1 cut(s) 286
CciI TCATGA 1 cut(s) 577
CfoI GCGC 1 cut(s) 1050
Cfr13I GGNCC 2 cut(s) 225, 877
ClaI ATCGAT 1 cut(s) 384
Csp6I GTAC 3 cut(s) 312, 611, 919
CviAII CATG 5 cut(s) 157, 578, 629, 793, 1076
CviQI GTAC 3 cut(s) 312, 611, 919
DdeI CTNAG 8 cut(s) 21, 287, 374, 453, 816, 1044, 1128, 1218
DpnI GATC 9 cut(s) 134, 203, 383, 402, 459, 519, 759, 836, 908
DpnII GATC 9 cut(s) 132, 201, 381, 400, 457, 517, 757, 834, 906
DriI GACNNNNNGTC 2 cut(s) 586, 1276
Eam1104I CTCTTC 2 cut(s) 207, 1171
Eam1105I GACNNNNNGTC 2 cut(s) 586, 1276
EarI CTCTTC 2 cut(s) 207, 1171
Ecl136II GAGCTC 1 cut(s) 53
Eco130I CCWWGG 2 cut(s) 156, 561
Eco24I GRGCYC 1 cut(s) 55
Eco31I GGTCTC 1 cut(s) 146
Eco47I GGWCC 1 cut(s) 225
Eco53kI GAGCTC 1 cut(s) 53
Eco57I CTGAAG 1 cut(s) 1221
EcoICRI GAGCTC 1 cut(s) 53
EcoNI CCTNNNNNAGG 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 877
EcoRII CCWGG 3 cut(s) 980, 1012, 1024
EcoT14I CCWWGG 2 cut(s) 156, 561
EcoT38I GRGCYC 1 cut(s) 55
ErhI CCWWGG 2 cut(s) 156, 561
FaeI CATG 5 cut(s) 160, 581, 632, 796, 1079
FalI AAGNNNNNCTT 2 cut(s) 684, 716
FatI CATG 5 cut(s) 156, 577, 628, 792, 1075
FauI CCCGC 1 cut(s) 591
FauNDI CATATG 1 cut(s) 571
FbaI TGATCA 1 cut(s) 201
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 4 cut(s) 1117, 1172, 1207, 1285
FokI GGATG 5 cut(s) 391, 877, 998, 1006, 1198
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 4 cut(s) 1117, 1172, 1207, 1285
GlaI GCGC 1 cut(s) 1049
GluI GCNGC 4 cut(s) 1117, 1172, 1207, 1285
GsuI CTGGAG 2 cut(s) 170, 1035
HaeIII GGCC 2 cut(s) 143, 878
HapII CCGG 1 cut(s) 119
HhaI GCGC 1 cut(s) 1050
Hin1II CATG 5 cut(s) 160, 581, 632, 796, 1079
Hin6I GCGC 1 cut(s) 1048
HinP1I GCGC 1 cut(s) 1048
HindIII AAGCTT 1 cut(s) 933
HinfI GANTC 9 cut(s) 13, 100, 122, 289, 449, 566, 812, 951, 1256
HpaII CCGG 1 cut(s) 119
HphI GGTGA 2 cut(s) 286, 970
Hpy166II GTNNAC 2 cut(s) 558, 790
Hpy188I TCNGA 6 cut(s) 229, 340, 352, 454, 762, 1201
Hpy188III TCNNGA 6 cut(s) 149, 293, 404, 578, 890, 948
Hpy8I GTNNAC 2 cut(s) 558, 790
Hpy99I CGWCG 1 cut(s) 140
HpyCH4III ACNGT 1 cut(s) 787
HpyCH4V TGCA 8 cut(s) 106, 197, 347, 427, 553, 628, 807, 974
HpyF10VI GCNNNNNNNGC 1 cut(s) 550
HpyF3I CTNAG 8 cut(s) 21, 287, 374, 453, 816, 1044, 1128, 1218
Hsp92II CATG 5 cut(s) 160, 581, 632, 796, 1079
HspAI GCGC 1 cut(s) 1048
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 9 cut(s) 132, 201, 381, 400, 457, 517, 757, 834, 906
LguI GCTCTTC 1 cut(s) 1171
LmnI GCTCC 3 cut(s) 50, 163, 635
Lsp1109I GCAGC 3 cut(s) 1128, 1183, 1296
LweI GCATC 3 cut(s) 356, 360, 855
MaeIII GTNAC 1 cut(s) 647
MalI GATC 9 cut(s) 134, 203, 383, 402, 459, 519, 759, 836, 908
MbiI CCGCTC 1 cut(s) 1127
MboI GATC 9 cut(s) 132, 201, 381, 400, 457, 517, 757, 834, 906
MboII GAAGA 9 cut(s) 224, 262, 265, 695, 788, 905, 935, 1073, 1158
MfeI CAATTG 1 cut(s) 428
MflI RGATCY 3 cut(s) 400, 457, 757
MhlI GDGCHC 1 cut(s) 55
MluCI AATT 7 cut(s) 169, 181, 319, 428, 926, 1096, 1292
MlyI GAGTC 4 cut(s) 298, 458, 575, 821
MmeI TCCRAC 5 cut(s) 282, 342, 925, 979, 1059
MseI TTAA 4 cut(s) 38, 237, 660, 828
MspA1I CMGCKG 3 cut(s) 586, 1191, 1217
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 4 cut(s) 119, 982, 1014, 1026
MunI CAATTG 1 cut(s) 428
MvaI CCWGG 3 cut(s) 982, 1014, 1026
MvnI CGCG 1 cut(s) 1050
MwoI GCNNNNNNNGC 1 cut(s) 550
NciI CCSGG 1 cut(s) 119
NcoI CCATGG 1 cut(s) 156
NdeI CATATG 1 cut(s) 571
NdeII GATC 9 cut(s) 132, 201, 381, 400, 457, 517, 757, 834, 906
NlaIII CATG 5 cut(s) 160, 581, 632, 796, 1079
NlaIV GGNNCC 4 cut(s) 165, 226, 402, 1180
NmuCI GTSAC 1 cut(s) 647
NspI RCATGY 1 cut(s) 796
PagI TCATGA 1 cut(s) 577
PciSI GCTCTTC 1 cut(s) 1171
PfeI GAWTC 5 cut(s) 13, 100, 122, 951, 1256
PkrI GCNGC 4 cut(s) 1118, 1173, 1208, 1286
PleI GAGTC 4 cut(s) 297, 457, 574, 820
PpsI GAGTC 4 cut(s) 297, 457, 574, 820
Psp124BI GAGCTC 1 cut(s) 55
Psp6I CCWGG 3 cut(s) 980, 1012, 1024
PspGI CCWGG 3 cut(s) 980, 1012, 1024
PspN4I GGNNCC 4 cut(s) 165, 226, 402, 1180
PspPI GGNCC 2 cut(s) 225, 877
PstNI CAGNNNCTG 1 cut(s) 286
PsuI RGATCY 3 cut(s) 400, 457, 757
RsaI GTAC 3 cut(s) 313, 612, 920
RsaNI GTAC 3 cut(s) 312, 611, 919
SacI GAGCTC 1 cut(s) 55
SapI GCTCTTC 1 cut(s) 1171
SaqAI TTAA 4 cut(s) 38, 237, 660, 828
SatI GCNGC 4 cut(s) 1117, 1172, 1207, 1285
Sau3AI GATC 9 cut(s) 132, 201, 381, 400, 457, 517, 757, 834, 906
Sau96I GGNCC 2 cut(s) 225, 877
SchI GAGTC 4 cut(s) 298, 458, 575, 821
ScrFI CCNGG 4 cut(s) 119, 982, 1014, 1026
SduI GDGCHC 1 cut(s) 55
SfaNI GCATC 3 cut(s) 356, 360, 855
SinI GGWCC 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
Sse9I AATT 7 cut(s) 169, 181, 319, 428, 926, 1096, 1292
SsiI CCGC 7 cut(s) 392, 584, 1125, 1191, 1206, 1215, 1262
SstI GAGCTC 1 cut(s) 55
StyD4I CCNGG 4 cut(s) 117, 980, 1012, 1024
StyI CCWWGG 2 cut(s) 156, 561
TaaI ACNGT 1 cut(s) 787
TaqI TCGA 3 cut(s) 64, 135, 384
TasI AATT 7 cut(s) 169, 181, 319, 428, 926, 1096, 1292
TatI WGTACW 1 cut(s) 311
TauI GCSGC 1 cut(s) 1209
TfiI GAWTC 5 cut(s) 13, 100, 122, 951, 1256
Tru1I TTAA 4 cut(s) 38, 237, 660, 828
Tru9I TTAA 4 cut(s) 38, 237, 660, 828
TscAI CASTG 2 cut(s) 1048, 1108
TseFI GTSAC 1 cut(s) 647
TseI GCWGC 3 cut(s) 1116, 1171, 1284
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 8 cut(s) 98, 107, 194, 332, 470, 566, 873, 1125
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 2 cut(s) 1048, 1108
VpaK11BI GGWCC 1 cut(s) 225
XagI CCTNNNNNAGG 1 cut(s) 618
XapI RAATTY 2 cut(s) 181, 1096
XceI RCATGY 1 cut(s) 796
XcmI CCANNNNNNNNNTGG 1 cut(s) 1076
XmiI GTMKAC 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.