pycom05g21250

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013249
Physical Location & Seq
Forward (+)
23909956 .. 23910952
997 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21250.1

Sequence Viewer

Length: 783 bp
ATGGCTTTTCTGCTACCAAATTTCTCTGCTTCCTCCTTACTCCTCCAAACCAAACCAAAAGAAAAACCCTTTCACCAAATGCTCTCCCAGACAAGACCCAGTTTTTCTTTCTCTCACCTCTCTCCTCTGGCCAGCGTACAGACGCCCACTTTGTTGGAGGGTGCGCAGCTTGACACCTCCAGGGTTGCGCAGGATAAGCAGCAAAGGGACGATTTTTATGTCAATCTTGGCCTGGCTGTACGGACGCTTCGTGAAGATGTCCCTTTAATTTTCGCCAAGGACCTTAATTATGACATTTATAGGGATGATATAACATTTATGGATCCTTTAAACACATTCACTGGCATTGAGAATTACAGATCGATCTTCTGGGCATTGCGATTTCATGGAAAGATTCTGTTCCGCGATATCTCCCTCGAGGTTTATAGGATTTGGCAGCCTTCAGAGAATGTGATACTGATAAGGTGGAACATGAAGGGTGTTCCTCGAGTTCCATGGGAAGCGAAGGGTCAGTTTCAGGGCACCTCGCGGTATAAATTGGACCGAAAGGGCAAAATATATGAACATAAAGTTGATAACTTGGCGTTTAACTTTCCACAGAAGCTCAAACCAGCTGCATCAGTGTTGGATTTAATGGCACCAGGCCCTGCTAGTCCTAATCTGACATTTCTGTGGGGTCCTGCAGATGCGTACTCATCTTCGTGGTTGGAGTTTTATCGAGCAGTGAGGGGGACTTTGAATCGGGATGGTTACTTGCTTCCACAAGATGGTTTGGTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

261

Amino Acids

29.88

Weight (kDa)

9.3

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 79 - 189 1.8e-39 Uncharacterized conserved protein (DUF2358)
SnoaL_2 PF12680 98 - 189 4.3e-07 SnoaL-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79510 AT1G79510
fragaria_vesca FvH4_3g14540
malus_domestica MD05G1235700.v1.1 MD10G1212200.v1.1
prunus_persica Prupe.4G130800_v2.0.a1
pyrus_communis pycom05g21250 pycom10g17980
rosa_chinensis RchiOBHm_Chr5g0023911
rosa_laevigata RLG00000032748
rosa_multiflora Rmu_sc0004175.1_g000013
rosa_roxburghii Rroxscaffold_1G00055300
rosa_rugosa Rorug05G0080300
rosa_samantha Rh5AG170600 Rh5BG167700 Rh5CG183200 Rh5DG169800
rosa_wichuraiana Rw5G015430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 416
Acc16I TGCGCA 2 cut(s) 165, 189
AccB1I GGYRCC 2 cut(s) 521, 637
AccB7I CCANNNNNTGG 1 cut(s) 767
AccII CGCG 2 cut(s) 405, 529
AciI CCGC 2 cut(s) 403, 529
AclWI GGATC 2 cut(s) 317, 330
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 3 cut(s) 138, 240, 692
AfiI CCNNNNNNNGG 1 cut(s) 767
AgsI TTSAA 1 cut(s) 739
AjnI CCWGG 3 cut(s) 179, 231, 640
AluBI AGCT 4 cut(s) 169, 604, 614, 780
AluI AGCT 4 cut(s) 169, 604, 614, 780
AlwI GGATC 2 cut(s) 317, 330
AlwNI CAGNNNCTG 1 cut(s) 647
Ama87I CYCGRG 2 cut(s) 416, 486
AoxI GGCC 3 cut(s) 129, 229, 643
ApeKI GCWGC 4 cut(s) 166, 199, 436, 614
ApoI RAATTY 1 cut(s) 19
ArsI GACNNNNNNTTYG 2 cut(s) 133, 165
AspLEI GCGC 2 cut(s) 166, 190
AspS9I GGNCC 4 cut(s) 280, 541, 644, 677
AsuHPI GGTGA 2 cut(s) 65, 107
AvaI CYCGRG 2 cut(s) 416, 486
AvaII GGWCC 3 cut(s) 280, 541, 677
BaeGI GKGCMC 1 cut(s) 524
BalI TGGCCA 1 cut(s) 131
BamHI GGATCC 1 cut(s) 322
BanI GGYRCC 2 cut(s) 521, 637
BarI GAAGNNNNNNTAC 2 cut(s) 231, 263
BbvI GCAGC 4 cut(s) 178, 211, 448, 601
BccI CCATC 2 cut(s) 740, 761
BciT130I CCWGG 3 cut(s) 181, 233, 642
BfaI CTAG 1 cut(s) 651
BfmI CTRYAG 1 cut(s) 681
BisI GCNGC 4 cut(s) 167, 200, 437, 615
BlsI GCNGC 4 cut(s) 168, 201, 438, 616
Bme1390I CCNGG 3 cut(s) 181, 233, 642
Bme18I GGWCC 3 cut(s) 280, 541, 677
BmeT110I CYCGRG 2 cut(s) 416, 486
BmgT120I GGNCC 4 cut(s) 280, 541, 644, 677
BmiI GGNNCC 4 cut(s) 324, 523, 639, 678
BmrFI CCNGG 3 cut(s) 181, 233, 642
BmrI ACTGGG 1 cut(s) 93
BmsI GCATC 2 cut(s) 626, 676
BmuI ACTGGG 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 163
Bsa29I ATCGAT 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 3 cut(s) 180, 276, 494
Bsc4I CCNNNNNNNGG 1 cut(s) 767
Bse1I ACTGG 2 cut(s) 99, 346
Bse3DI GCAATG 1 cut(s) 374
BseBI CCWGG 3 cut(s) 181, 233, 642
BseCI ATCGAT 1 cut(s) 362
BseDI CCNNGG 3 cut(s) 180, 276, 494
BseGI GGATG 2 cut(s) 310, 751
BseLI CCNNNNNNNGG 1 cut(s) 767
BseMI GCAATG 1 cut(s) 374
BseNI ACTGG 2 cut(s) 99, 346
BseRI GAGGAG 2 cut(s) 32, 114
BseSI GKGCMC 1 cut(s) 524
BseXI GCAGC 4 cut(s) 178, 211, 448, 601
Bsh1236I CGCG 2 cut(s) 405, 529
BshFI GGCC 3 cut(s) 131, 231, 645
BshNI GGYRCC 2 cut(s) 521, 637
BshVI ATCGAT 1 cut(s) 362
BsiHKCI CYCGRG 2 cut(s) 416, 486
BslFI GGGAC 3 cut(s) 221, 245, 745
BslI CCNNNNNNNGG 1 cut(s) 767
BsmFI GGGAC 3 cut(s) 221, 245, 745
BsnI GGCC 3 cut(s) 131, 231, 645
BsoBI CYCGRG 2 cut(s) 416, 486
Bsp1286I GDGCHC 1 cut(s) 524
Bsp143I GATC 3 cut(s) 322, 359, 363
Bsp19I CCATGG 1 cut(s) 494
BspACI CCGC 2 cut(s) 403, 529
BspANI GGCC 3 cut(s) 131, 231, 645
BspDI ATCGAT 1 cut(s) 362
BspFNI CGCG 2 cut(s) 405, 529
BspLI GGNNCC 4 cut(s) 324, 523, 639, 678
BspMAI CTGCAG 1 cut(s) 685
BspPI GGATC 2 cut(s) 317, 330
BspT107I GGYRCC 2 cut(s) 521, 637
BsrDI GCAATG 1 cut(s) 374
BsrI ACTGG 2 cut(s) 99, 346
BssECI CCNNGG 3 cut(s) 180, 276, 494
BssMI GATC 3 cut(s) 322, 359, 363
BssNI GRCGYC 1 cut(s) 143
BssT1I CCWWGG 2 cut(s) 276, 494
Bst2UI CCWGG 3 cut(s) 181, 233, 642
BstACI GRCGYC 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 133
BstDSI CCRYGG 1 cut(s) 494
BstF5I GGATG 2 cut(s) 310, 751
BstFNI CGCG 2 cut(s) 405, 529
BstHHI GCGC 2 cut(s) 166, 190
BstKTI GATC 3 cut(s) 325, 362, 366
BstMBI GATC 3 cut(s) 322, 359, 363
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstNI CCWGG 3 cut(s) 181, 233, 642
BstSCI CCNGG 3 cut(s) 179, 231, 640
BstSFI CTRYAG 1 cut(s) 681
BstSLI GKGCMC 1 cut(s) 524
BstUI CGCG 2 cut(s) 405, 529
BstV1I GCAGC 4 cut(s) 178, 211, 448, 601
BstX2I RGATCY 1 cut(s) 322
BstXI CCANNNNNNTGG 1 cut(s) 154
BstYI RGATCY 1 cut(s) 322
Bsu15I ATCGAT 1 cut(s) 362
BsuRI GGCC 3 cut(s) 131, 231, 645
BsuTUI ATCGAT 1 cut(s) 362
BtgI CCRYGG 1 cut(s) 494
BtsCI GGATG 2 cut(s) 310, 751
BtsI GCAGTG 1 cut(s) 729
BtsIMutI CAGTG 3 cut(s) 339, 627, 729
Cac8I GCNNGC 1 cut(s) 133
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 2 cut(s) 166, 190
Cfr13I GGNCC 4 cut(s) 280, 541, 644, 677
ClaI ATCGAT 1 cut(s) 362
CseI GACGC 2 cut(s) 151, 253
Csp6I GTAC 3 cut(s) 137, 239, 691
CviAII CATG 3 cut(s) 386, 472, 495
CviQI GTAC 3 cut(s) 137, 239, 691
DpnI GATC 3 cut(s) 324, 361, 365
DpnII GATC 3 cut(s) 322, 359, 363
DraI TTTAAA 1 cut(s) 330
EaeI YGGCCR 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 276, 494
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 3 cut(s) 280, 541, 677
Eco57I CTGAAG 1 cut(s) 426
Eco88I CYCGRG 2 cut(s) 416, 486
EcoO109I RGGNCCY 3 cut(s) 280, 644, 677
EcoRII CCWGG 3 cut(s) 179, 231, 640
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 2 cut(s) 276, 494
ErhI CCWWGG 2 cut(s) 276, 494
FaeI CATG 3 cut(s) 389, 475, 498
FaqI GGGAC 3 cut(s) 221, 245, 745
FatI CATG 3 cut(s) 385, 471, 494
Fnu4HI GCNGC 4 cut(s) 167, 200, 437, 615
FokI GGATG 2 cut(s) 317, 758
Fsp4HI GCNGC 4 cut(s) 167, 200, 437, 615
FspBI CTAG 1 cut(s) 651
FspI TGCGCA 2 cut(s) 165, 189
GlaI GCGC 2 cut(s) 165, 189
GluI GCNGC 4 cut(s) 167, 200, 437, 615
GsuI CTGGAG 1 cut(s) 163
HaeIII GGCC 3 cut(s) 131, 231, 645
HgaI GACGC 2 cut(s) 151, 253
HhaI GCGC 2 cut(s) 166, 190
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 3 cut(s) 389, 475, 498
Hin6I GCGC 2 cut(s) 164, 188
HinP1I GCGC 2 cut(s) 164, 188
HinfI GANTC 2 cut(s) 394, 739
HphI GGTGA 2 cut(s) 65, 107
Hpy188I TCNGA 2 cut(s) 445, 663
Hpy188III TCNNGA 2 cut(s) 251, 743
HpyAV CCTTC 3 cut(s) 450, 469, 499
HpyCH4V TGCA 2 cut(s) 617, 683
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 3 cut(s) 389, 475, 498
HspAI GCGC 2 cut(s) 164, 188
Kzo9I GATC 3 cut(s) 322, 359, 363
Lsp1109I GCAGC 4 cut(s) 178, 211, 448, 601
LweI GCATC 2 cut(s) 626, 676
MaeI CTAG 1 cut(s) 651
MaeIII GTNAC 1 cut(s) 749
MalI GATC 3 cut(s) 324, 361, 365
MboI GATC 3 cut(s) 322, 359, 363
MboII GAAGA 3 cut(s) 266, 358, 690
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 1 cut(s) 524
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 5 cut(s) 19, 267, 286, 352, 536
MluNI TGGCCA 1 cut(s) 131
MmeI TCCRAC 3 cut(s) 135, 606, 687
Mox20I TGGCCA 1 cut(s) 131
MscI TGGCCA 1 cut(s) 131
MseI TTAA 5 cut(s) 266, 285, 329, 588, 632
MslI CAYNNNNRTG 2 cut(s) 670, 700
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 614
MspR9I CCNGG 3 cut(s) 181, 233, 642
MvaI CCWGG 3 cut(s) 181, 233, 642
MvnI CGCG 2 cut(s) 405, 529
MwoI GCNNNNNNNGC 1 cut(s) 196
NcoI CCATGG 1 cut(s) 494
NdeII GATC 3 cut(s) 322, 359, 363
NlaIII CATG 3 cut(s) 389, 475, 498
NlaIV GGNNCC 4 cut(s) 324, 523, 639, 678
NsbI TGCGCA 2 cut(s) 165, 189
PaeR7I CTCGAG 2 cut(s) 416, 486
PcsI WCGNNNNNNNCGW 1 cut(s) 247
PfeI GAWTC 2 cut(s) 394, 739
PflMI CCANNNNNTGG 1 cut(s) 767
PkrI GCNGC 4 cut(s) 168, 201, 438, 616
PpuMI RGGWCCY 2 cut(s) 280, 677
Psp5II RGGWCCY 2 cut(s) 280, 677
Psp6I CCWGG 3 cut(s) 179, 231, 640
PspGI CCWGG 3 cut(s) 179, 231, 640
PspN4I GGNNCC 4 cut(s) 324, 523, 639, 678
PspPI GGNCC 4 cut(s) 280, 541, 644, 677
PspPPI RGGWCCY 2 cut(s) 280, 677
PspXI VCTCGAGB 2 cut(s) 416, 486
PstI CTGCAG 1 cut(s) 685
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 3 cut(s) 138, 240, 692
RsaNI GTAC 3 cut(s) 137, 239, 691
RseI CAYNNNNRTG 2 cut(s) 670, 700
SaqAI TTAA 5 cut(s) 266, 285, 329, 588, 632
SatI GCNGC 4 cut(s) 167, 200, 437, 615
Sau3AI GATC 3 cut(s) 322, 359, 363
Sau96I GGNCC 4 cut(s) 280, 541, 644, 677
ScrFI CCNGG 3 cut(s) 181, 233, 642
SduI GDGCHC 1 cut(s) 524
SfaNI GCATC 2 cut(s) 626, 676
SfcI CTRYAG 1 cut(s) 681
Sfr274I CTCGAG 2 cut(s) 416, 486
SinI GGWCC 3 cut(s) 280, 541, 677
SlaI CTCGAG 2 cut(s) 416, 486
SmiMI CAYNNNNRTG 2 cut(s) 670, 700
SmlI CTYRAG 2 cut(s) 416, 486
SmoI CTYRAG 2 cut(s) 416, 486
Sse9I AATT 5 cut(s) 19, 267, 286, 352, 536
SsiI CCGC 2 cut(s) 403, 529
SspMI CTAG 1 cut(s) 651
StyD4I CCNGG 3 cut(s) 179, 231, 640
StyI CCWWGG 2 cut(s) 276, 494
TaqI TCGA 4 cut(s) 362, 417, 487, 718
TaqII GACCGA 1 cut(s) 558
TasI AATT 5 cut(s) 19, 267, 286, 352, 536
TfiI GAWTC 2 cut(s) 394, 739
Tru1I TTAA 5 cut(s) 266, 285, 329, 588, 632
Tru9I TTAA 5 cut(s) 266, 285, 329, 588, 632
TscAI CASTG 3 cut(s) 346, 627, 729
TseI GCWGC 4 cut(s) 166, 199, 436, 614
TspDTI ATGAA 3 cut(s) 374, 488, 576
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 3 cut(s) 346, 627, 729
Van91I CCANNNNNTGG 1 cut(s) 767
VpaK11BI GGWCC 3 cut(s) 280, 541, 677
XapI RAATTY 1 cut(s) 19
XhoI CTCGAG 2 cut(s) 416, 486
XspI CTAG 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.