RLG00000032748

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013249
Physical Location & Seq
Reverse (-)
18583738 .. 18584954
1217 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032748

Sequence Viewer

Length: 780 bp
ATGGCTTTTCTACTACCAAATTTCTCTCCCACTTTACCCCTCCAAACCAAGTTTAAGGAAAAACCAATTCACCACACACTTTCTCAGATGAAACCCAACTACTCTCTCCCTCCTCTCTCTCACCTGGCCAGTGTTCAGACCCCAACTTTGTCAGAGGATGCGCAGGTTAACACCTCCAAGGGTGCGCAGGATAAGCAGCAAAGAGATGATTTTTATGTCAATCTTGGCTTGGCTGTTCGAACCCTTCGTGAAGATATGCCTTTGATTTTCGCTGAAGATCTTAATTATGACATTTACAGGGATGATATAACATTTATGGATCCTTTGAACACATTCACCGGCATTGAAAACTACAAGTTGATCTTCTGGGCATTGAGATTTCATGGCAAGATTCTGTTCCGTGACATTTCCCTTGAGGTTTTTAGGATTTGGCAGCCTTCGGAGAATGTCATATTGATTAGGTGGAATATGAAGGGTGTTCCACGGGTTCCGTGGGAAGCAAAGGGCCAGTTTCAGGGCACTTCGCGGTATAAATTGGATCGGAAAGGCAAGATTTATGAACACAAGGTTGATAACTTGGCGTTTAACTTCCCACAACAGCTCAAACCAGCATCATCAGTGTTGGATTTAGTAGCAGCATGCCCAGCTAGCCCTAACTTGACATTTTTGTGGGGTCCTGCGGATCCTTACTCATCTTCGTGGTTGGAGTTCTATCGAGCAGTCAGGGGGACTTTGGATCGAGAAACTTTCTTGCTTCCACAAGATGGTTTGGCTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

260

Amino Acids

29.84

Weight (kDa)

7.81

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 78 - 188 6.9e-40 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79510 AT1G79510
fragaria_vesca FvH4_3g14540
malus_domestica MD05G1235700.v1.1 MD10G1212200.v1.1
prunus_persica Prupe.4G130800_v2.0.a1
pyrus_communis pycom05g21250 pycom10g17980
rosa_chinensis RchiOBHm_Chr5g0023911
rosa_laevigata RLG00000032748
rosa_multiflora Rmu_sc0004175.1_g000013
rosa_roxburghii Rroxscaffold_1G00055300
rosa_rugosa Rorug05G0080300
rosa_samantha Rh5AG170600 Rh5BG167700 Rh5CG183200 Rh5DG169800
rosa_wichuraiana Rw5G015430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 162, 186
Acc36I ACCTGC 1 cut(s) 154
AccB7I CCANNNNNTGG 1 cut(s) 764
AccII CGCG 1 cut(s) 526
AciI CCGC 2 cut(s) 526, 680
AclWI GGATC 6 cut(s) 314, 327, 546, 677, 690, 744
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 1 cut(s) 294
AfiI CCNNNNNNNGG 2 cut(s) 514, 764
AgsI TTSAA 2 cut(s) 328, 347
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 3 cut(s) 601, 647, 777
AluI AGCT 3 cut(s) 601, 647, 777
AlwI GGATC 6 cut(s) 314, 327, 546, 677, 690, 744
AoxI GGCC 2 cut(s) 126, 505
ApeKI GCWGC 3 cut(s) 196, 433, 635
ApoI RAATTY 1 cut(s) 19
ArsI GACNNNNNNTTYG 2 cut(s) 130, 162
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 2 cut(s) 163, 187
AspS9I GGNCC 2 cut(s) 505, 674
AsuHPI GGTGA 3 cut(s) 62, 113, 328
AsuII TTCGAA 1 cut(s) 238
AsuNHI GCTAGC 2 cut(s) 647, 773
AvaII GGWCC 1 cut(s) 674
BaeGI GKGCMC 1 cut(s) 521
BalI TGGCCA 1 cut(s) 128
BamHI GGATCC 2 cut(s) 319, 682
BbvI GCAGC 3 cut(s) 208, 445, 647
BccI CCATC 1 cut(s) 758
BciT130I CCWGG 1 cut(s) 125
BfaI CTAG 2 cut(s) 648, 774
BfuAI ACCTGC 1 cut(s) 154
BglII AGATCT 1 cut(s) 277
BisI GCNGC 3 cut(s) 197, 434, 636
BlsI GCNGC 3 cut(s) 198, 435, 637
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 674
BmgT120I GGNCC 2 cut(s) 505, 674
BmiI GGNNCC 4 cut(s) 321, 489, 675, 684
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 148, 620
BmtI GCTAGC 2 cut(s) 651, 777
Bpu14I TTCGAA 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 434
BsaJI CCNNGG 3 cut(s) 177, 482, 491
Bsc4I CCNNNNNNNGG 2 cut(s) 514, 764
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 2 cut(s) 129, 508
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 3 cut(s) 177, 482, 491
BseGI GGATG 2 cut(s) 163, 307
BseLI CCNNNNNNNGG 2 cut(s) 514, 764
BseMII CTCAG 1 cut(s) 98
BseNI ACTGG 2 cut(s) 129, 508
BseRI GAGGAG 1 cut(s) 102
BseSI GKGCMC 1 cut(s) 521
BseXI GCAGC 3 cut(s) 208, 445, 647
BseYI CCCAGC 1 cut(s) 643
Bsh1236I CGCG 1 cut(s) 526
BshFI GGCC 2 cut(s) 128, 507
BsiSI CCGG 1 cut(s) 339
BslFI GGGAC 1 cut(s) 742
BslI CCNNNNNNNGG 2 cut(s) 514, 764
BsmFI GGGAC 1 cut(s) 742
BsnI GGCC 2 cut(s) 128, 507
Bsp119I TTCGAA 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 521
Bsp143I GATC 6 cut(s) 277, 319, 360, 538, 682, 736
BspACI CCGC 2 cut(s) 526, 680
BspANI GGCC 2 cut(s) 128, 507
BspCNI CTCAG 1 cut(s) 97
BspFNI CGCG 1 cut(s) 526
BspLI GGNNCC 4 cut(s) 321, 489, 675, 684
BspMI ACCTGC 1 cut(s) 154
BspOI GCTAGC 2 cut(s) 651, 777
BspPI GGATC 6 cut(s) 314, 327, 546, 677, 690, 744
BspT104I TTCGAA 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 2 cut(s) 129, 508
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 3 cut(s) 177, 482, 491
BssMI GATC 6 cut(s) 277, 319, 360, 538, 682, 736
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 238
BstC8I GCNNGC 3 cut(s) 640, 649, 775
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 2 cut(s) 482, 491
BstF5I GGATG 2 cut(s) 163, 307
BstFNI CGCG 1 cut(s) 526
BstHHI GCGC 2 cut(s) 163, 187
BstKTI GATC 6 cut(s) 280, 322, 363, 541, 685, 739
BstMBI GATC 6 cut(s) 277, 319, 360, 538, 682, 736
BstMWI GCNNNNNNNGC 3 cut(s) 193, 644, 648
BstNI CCWGG 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 642
BstSCI CCNGG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 521
BstUI CGCG 1 cut(s) 526
BstV1I GCAGC 3 cut(s) 208, 445, 647
BstX2I RGATCY 3 cut(s) 277, 319, 682
BstYI RGATCY 3 cut(s) 277, 319, 682
BsuRI GGCC 2 cut(s) 128, 507
BtgI CCRYGG 2 cut(s) 482, 491
BtsCI GGATG 2 cut(s) 163, 307
BtsIMutI CAGTG 2 cut(s) 136, 624
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 3 cut(s) 640, 649, 775
CfoI GCGC 2 cut(s) 163, 187
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 505, 674
CviAII CATG 2 cut(s) 383, 639
DdeI CTNAG 1 cut(s) 84
DpnI GATC 6 cut(s) 279, 321, 362, 540, 684, 738
DpnII GATC 6 cut(s) 277, 319, 360, 538, 682, 736
EaeI YGGCCR 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 674
Eco57I CTGAAG 1 cut(s) 294
EcoO109I RGGNCCY 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 2 cut(s) 386, 642
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 1 cut(s) 742
FatI CATG 2 cut(s) 382, 638
Fnu4HI GCNGC 3 cut(s) 197, 434, 636
FokI GGATG 2 cut(s) 170, 314
Fsp4HI GCNGC 3 cut(s) 197, 434, 636
FspBI CTAG 2 cut(s) 648, 774
FspI TGCGCA 2 cut(s) 162, 186
GlaI GCGC 2 cut(s) 162, 186
GluI GCNGC 3 cut(s) 197, 434, 636
GsaI CCCAGC 1 cut(s) 647
HaeIII GGCC 2 cut(s) 128, 507
HapII CCGG 1 cut(s) 339
HhaI GCGC 2 cut(s) 163, 187
Hin1II CATG 2 cut(s) 386, 642
Hin6I GCGC 2 cut(s) 161, 185
HinP1I GCGC 2 cut(s) 161, 185
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HinfI GANTC 1 cut(s) 391
HpaI GTTAAC 1 cut(s) 169
HpaII CCGG 1 cut(s) 339
HphI GGTGA 3 cut(s) 62, 113, 328
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 5 cut(s) 87, 138, 154, 442, 543
Hpy188III TCNNGA 2 cut(s) 248, 740
Hpy8I GTNNAC 1 cut(s) 169
HpyAV CCTTC 3 cut(s) 254, 447, 466
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 644, 648
HpyF3I CTNAG 1 cut(s) 84
Hsp92II CATG 2 cut(s) 386, 642
HspAI GCGC 2 cut(s) 161, 185
KspAI GTTAAC 1 cut(s) 169
Kzo9I GATC 6 cut(s) 277, 319, 360, 538, 682, 736
Lsp1109I GCAGC 3 cut(s) 208, 445, 647
LweI GCATC 2 cut(s) 148, 620
MaeI CTAG 2 cut(s) 648, 774
MaeIII GTNAC 1 cut(s) 401
MalI GATC 6 cut(s) 279, 321, 362, 540, 684, 738
MboI GATC 6 cut(s) 277, 319, 360, 538, 682, 736
MboII GAAGA 4 cut(s) 263, 287, 355, 687
MflI RGATCY 3 cut(s) 277, 319, 682
MhlI GDGCHC 1 cut(s) 521
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 4 cut(s) 19, 66, 283, 533
MluNI TGGCCA 1 cut(s) 128
MmeI TCCRAC 2 cut(s) 603, 684
MnlI CCTC 6 cut(s) 50, 120, 123, 148, 184, 409
Mox20I TGGCCA 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 332
MscI TGGCCA 1 cut(s) 128
MseI TTAA 4 cut(s) 54, 168, 282, 585
MslI CAYNNNNRTG 2 cut(s) 667, 697
Msp20I TGGCCA 1 cut(s) 128
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 1 cut(s) 526
MwoI GCNNNNNNNGC 3 cut(s) 193, 644, 648
NdeII GATC 6 cut(s) 277, 319, 360, 538, 682, 736
NheI GCTAGC 2 cut(s) 647, 773
NlaIII CATG 2 cut(s) 386, 642
NlaIV GGNNCC 4 cut(s) 321, 489, 675, 684
NmuCI GTSAC 1 cut(s) 401
NsbI TGCGCA 2 cut(s) 162, 186
NspI RCATGY 1 cut(s) 642
NspV TTCGAA 1 cut(s) 238
PaeI GCATGC 1 cut(s) 642
PcsI WCGNNNNNNNCGW 1 cut(s) 244
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 764
PkrI GCNGC 3 cut(s) 198, 435, 637
PpuMI RGGWCCY 1 cut(s) 674
Psp5II RGGWCCY 1 cut(s) 674
Psp6I CCWGG 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 4 cut(s) 321, 489, 675, 684
PspPI GGNCC 2 cut(s) 505, 674
PspPPI RGGWCCY 1 cut(s) 674
PsuI RGATCY 3 cut(s) 277, 319, 682
RseI CAYNNNNRTG 2 cut(s) 667, 697
SaqAI TTAA 4 cut(s) 54, 168, 282, 585
SatI GCNGC 3 cut(s) 197, 434, 636
Sau3AI GATC 6 cut(s) 277, 319, 360, 538, 682, 736
Sau96I GGNCC 2 cut(s) 505, 674
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 521
SetI ASST 9 cut(s) 126, 168, 176, 420, 464, 570, 603, 649, 779
SfaNI GCATC 2 cut(s) 148, 620
SfuI TTCGAA 1 cut(s) 238
SinI GGWCC 1 cut(s) 674
SmiMI CAYNNNNRTG 2 cut(s) 667, 697
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SphI GCATGC 1 cut(s) 642
Sse9I AATT 4 cut(s) 19, 66, 283, 533
SsiI CCGC 2 cut(s) 526, 680
SspMI CTAG 2 cut(s) 648, 774
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 1 cut(s) 177
TaqI TCGA 3 cut(s) 238, 715, 739
TasI AATT 4 cut(s) 19, 66, 283, 533
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 4 cut(s) 54, 168, 282, 585
Tru9I TTAA 4 cut(s) 54, 168, 282, 585
TscAI CASTG 2 cut(s) 136, 624
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 3 cut(s) 196, 433, 635
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 4 cut(s) 104, 371, 485, 573
TspGWI ACGGA 2 cut(s) 389, 480
TspRI CASTG 2 cut(s) 136, 624
Van91I CCANNNNNTGG 1 cut(s) 764
VpaK11BI GGWCC 1 cut(s) 674
XapI RAATTY 1 cut(s) 19
XceI RCATGY 1 cut(s) 642
XcmI CCANNNNNNNNNTGG 1 cut(s) 489
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 2 cut(s) 648, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.