Rmu_sc0004175.1_g000013

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004175.1
OG0013249
Physical Location & Seq
Forward (+)
59979 .. 61475
1497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004175.1_g000013.1.cds

Sequence Viewer

Length: 693 bp
atgaaacccaaccactctctccctcctgtctctcacctggccagtgttcagaccccaactttgtcagaggatgcgcaggttaacacctccaagggtgcgcaggataagcaggaaagagatgatttttatgtcaatcttggcgtggctgttcgaacccttcgtgaagatatgcctttgattttcgctgaagatcttaattatgacatttacagggatgatataacatttatggatcctttgaacacgttcactggcattgaaaactacaagttgatcttctgggcattgagatttcatgccaagattctgttccgtgacatttcccttgaggtttataggatttggcagccttcggagaatgtcatattgattaggtggaatatgaagggtgttccacgggttccgtgggaagcaaagggccagtttcagggcacgtcgcggtataaattggatcggaaaggcaagatttatgaacataaggttgataacttggcgtttaacttcccacaacagctcaaaccagcatcatcagtgttggatttagtagcagcatgcccagctagccctaacttgacatttttgtggggtcctgcggatgcttactcatcttcgtggttggagttctatcgagcagtcagggggactttggatcgagaaactttcttgcttccacaagatggtttggctagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

230

Amino Acids

26.45

Weight (kDa)

6.1

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79510 AT1G79510
fragaria_vesca FvH4_3g14540
malus_domestica MD05G1235700.v1.1 MD10G1212200.v1.1
prunus_persica Prupe.4G130800_v2.0.a1
pyrus_communis pycom05g21250 pycom10g17980
rosa_chinensis RchiOBHm_Chr5g0023911
rosa_laevigata RLG00000032748
rosa_multiflora Rmu_sc0004175.1_g000013
rosa_roxburghii Rroxscaffold_1G00055300
rosa_rugosa Rorug05G0080300
rosa_samantha Rh5AG170600 Rh5BG167700 Rh5CG183200 Rh5DG169800
rosa_wichuraiana Rw5G015430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 75, 99
Acc36I ACCTGC 1 cut(s) 67
AccB7I CCANNNNNTGG 1 cut(s) 677
AccII CGCG 1 cut(s) 439
AciI CCGC 2 cut(s) 439, 593
AclWI GGATC 4 cut(s) 227, 240, 459, 657
AcoI YGGCCR 1 cut(s) 39
AcuI CTGAAG 1 cut(s) 207
AfiI CCNNNNNNNGG 2 cut(s) 427, 677
AflIII ACRYGT 1 cut(s) 243
AgsI TTSAA 2 cut(s) 241, 260
AjiI CACGTC 1 cut(s) 435
AjnI CCWGG 1 cut(s) 36
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 3 cut(s) 514, 560, 690
AluI AGCT 3 cut(s) 514, 560, 690
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 4 cut(s) 227, 240, 459, 657
AoxI GGCC 2 cut(s) 39, 418
ApeKI GCWGC 2 cut(s) 346, 548
ArsI GACNNNNNNTTYG 2 cut(s) 43, 75
Asp700I GAANNNNTTC 1 cut(s) 245
AspLEI GCGC 2 cut(s) 76, 100
AspS9I GGNCC 2 cut(s) 418, 587
AsuHPI GGTGA 1 cut(s) 26
AsuII TTCGAA 1 cut(s) 151
AsuNHI GCTAGC 2 cut(s) 560, 686
AvaII GGWCC 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 434
BalI TGGCCA 1 cut(s) 41
BamHI GGATCC 1 cut(s) 232
BbvI GCAGC 2 cut(s) 358, 560
BccI CCATC 1 cut(s) 671
BciT130I CCWGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 34
BfaI CTAG 2 cut(s) 561, 687
BfuAI ACCTGC 1 cut(s) 67
BglII AGATCT 1 cut(s) 190
BisI GCNGC 2 cut(s) 347, 549
BlsI GCNGC 2 cut(s) 348, 550
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 1 cut(s) 587
BmgBI CACGTC 1 cut(s) 435
BmgT120I GGNCC 2 cut(s) 418, 587
BmiI GGNNCC 3 cut(s) 234, 402, 588
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 3 cut(s) 61, 533, 586
BmtI GCTAGC 2 cut(s) 564, 690
Bpu14I TTCGAA 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 347
BsaJI CCNNGG 3 cut(s) 90, 395, 404
Bsc4I CCNNNNNNNGG 2 cut(s) 427, 677
Bse1I ACTGG 3 cut(s) 42, 256, 421
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 3 cut(s) 90, 395, 404
BseGI GGATG 3 cut(s) 76, 220, 601
BseLI CCNNNNNNNGG 2 cut(s) 427, 677
BseNI ACTGG 3 cut(s) 42, 256, 421
BseSI GKGCMC 1 cut(s) 434
BseXI GCAGC 2 cut(s) 358, 560
BseYI CCCAGC 1 cut(s) 556
Bsh1236I CGCG 1 cut(s) 439
BshFI GGCC 2 cut(s) 41, 420
BslFI GGGAC 1 cut(s) 655
BslI CCNNNNNNNGG 2 cut(s) 427, 677
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 655
BsnI GGCC 2 cut(s) 41, 420
Bsp119I TTCGAA 1 cut(s) 151
Bsp1286I GDGCHC 1 cut(s) 434
Bsp143I GATC 5 cut(s) 190, 232, 273, 451, 649
BspACI CCGC 2 cut(s) 439, 593
BspANI GGCC 2 cut(s) 41, 420
BspFNI CGCG 1 cut(s) 439
BspLI GGNNCC 3 cut(s) 234, 402, 588
BspMI ACCTGC 1 cut(s) 67
BspOI GCTAGC 2 cut(s) 564, 690
BspPI GGATC 4 cut(s) 227, 240, 459, 657
BspT104I TTCGAA 1 cut(s) 151
BsrI ACTGG 3 cut(s) 42, 256, 421
BssECI CCNNGG 3 cut(s) 90, 395, 404
BssMI GATC 5 cut(s) 190, 232, 273, 451, 649
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 38
BstBI TTCGAA 1 cut(s) 151
BstC8I GCNNGC 3 cut(s) 553, 562, 688
BstDSI CCRYGG 2 cut(s) 395, 404
BstF5I GGATG 3 cut(s) 76, 220, 601
BstFNI CGCG 1 cut(s) 439
BstHHI GCGC 2 cut(s) 76, 100
BstKTI GATC 5 cut(s) 193, 235, 276, 454, 652
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 5 cut(s) 190, 232, 273, 451, 649
BstMWI GCNNNNNNNGC 3 cut(s) 106, 557, 561
BstNI CCWGG 1 cut(s) 38
BstNSI RCATGY 1 cut(s) 555
BstSCI CCNGG 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 434
BstUI CGCG 1 cut(s) 439
BstV1I GCAGC 2 cut(s) 358, 560
BstX2I RGATCY 2 cut(s) 190, 232
BstYI RGATCY 2 cut(s) 190, 232
BsuRI GGCC 2 cut(s) 41, 420
BtgI CCRYGG 2 cut(s) 395, 404
BtrI CACGTC 1 cut(s) 435
BtsCI GGATG 3 cut(s) 76, 220, 601
BtsIMutI CAGTG 3 cut(s) 49, 249, 537
BveI ACCTGC 1 cut(s) 67
Cac8I GCNNGC 3 cut(s) 553, 562, 688
CfoI GCGC 2 cut(s) 76, 100
Cfr13I GGNCC 2 cut(s) 418, 587
CviAII CATG 2 cut(s) 296, 552
CviJI RGCY 9 cut(s) 41, 146, 349, 420, 514, 560, 564, 686, 690
CviKI_1 RGCY 9 cut(s) 41, 146, 349, 420, 514, 560, 564, 686, 690
DpnI GATC 5 cut(s) 192, 234, 275, 453, 651
DpnII GATC 5 cut(s) 190, 232, 273, 451, 649
EaeI YGGCCR 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 207
EcoO109I RGGNCCY 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 2 cut(s) 299, 555
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FaqI GGGAC 1 cut(s) 655
FatI CATG 2 cut(s) 295, 551
Fnu4HI GCNGC 2 cut(s) 347, 549
FokI GGATG 3 cut(s) 83, 227, 608
Fsp4HI GCNGC 2 cut(s) 347, 549
FspBI CTAG 2 cut(s) 561, 687
FspI TGCGCA 2 cut(s) 75, 99
GlaI GCGC 2 cut(s) 75, 99
GluI GCNGC 2 cut(s) 347, 549
GsaI CCCAGC 1 cut(s) 560
HaeIII GGCC 2 cut(s) 41, 420
HhaI GCGC 2 cut(s) 76, 100
Hin1II CATG 2 cut(s) 299, 555
Hin6I GCGC 2 cut(s) 74, 98
HinP1I GCGC 2 cut(s) 74, 98
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 1 cut(s) 304
HpaI GTTAAC 1 cut(s) 82
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 2 cut(s) 82, 249
Hpy188I TCNGA 4 cut(s) 51, 67, 355, 456
Hpy188III TCNNGA 2 cut(s) 161, 653
Hpy8I GTNNAC 2 cut(s) 82, 249
Hpy99I CGWCG 1 cut(s) 439
HpyAV CCTTC 3 cut(s) 167, 360, 379
HpyCH4IV ACGT 2 cut(s) 245, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 557, 561
HpySE526I ACGT 2 cut(s) 245, 434
Hsp92II CATG 2 cut(s) 299, 555
HspAI GCGC 2 cut(s) 74, 98
KspAI GTTAAC 1 cut(s) 82
Kzo9I GATC 5 cut(s) 190, 232, 273, 451, 649
Lsp1109I GCAGC 2 cut(s) 358, 560
LweI GCATC 3 cut(s) 61, 533, 586
MaeI CTAG 2 cut(s) 561, 687
MaeII ACGT 2 cut(s) 245, 434
MaeIII GTNAC 1 cut(s) 314
MalI GATC 5 cut(s) 192, 234, 275, 453, 651
MboI GATC 5 cut(s) 190, 232, 273, 451, 649
MboII GAAGA 4 cut(s) 176, 200, 268, 600
MflI RGATCY 2 cut(s) 190, 232
MhlI GDGCHC 1 cut(s) 434
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 2 cut(s) 196, 446
MluNI TGGCCA 1 cut(s) 41
MmeI TCCRAC 2 cut(s) 516, 597
MnlI CCTC 4 cut(s) 33, 61, 97, 322
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 245
MscI TGGCCA 1 cut(s) 41
MseI TTAA 3 cut(s) 81, 195, 498
MslI CAYNNNNRTG 2 cut(s) 580, 610
Msp20I TGGCCA 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 38
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 3 cut(s) 106, 557, 561
NdeII GATC 5 cut(s) 190, 232, 273, 451, 649
NheI GCTAGC 2 cut(s) 560, 686
NlaIII CATG 2 cut(s) 299, 555
NlaIV GGNNCC 3 cut(s) 234, 402, 588
NmuCI GTSAC 1 cut(s) 314
NsbI TGCGCA 2 cut(s) 75, 99
NspI RCATGY 1 cut(s) 555
NspV TTCGAA 1 cut(s) 151
PaeI GCATGC 1 cut(s) 555
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PdmI GAANNNNTTC 1 cut(s) 245
PfeI GAWTC 1 cut(s) 304
PflMI CCANNNNNTGG 1 cut(s) 677
PkrI GCNGC 2 cut(s) 348, 550
PpuMI RGGWCCY 1 cut(s) 587
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 36
PspFI CCCAGC 1 cut(s) 556
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 3 cut(s) 234, 402, 588
PspPI GGNCC 2 cut(s) 418, 587
PspPPI RGGWCCY 1 cut(s) 587
PsuI RGATCY 2 cut(s) 190, 232
RseI CAYNNNNRTG 2 cut(s) 580, 610
SaqAI TTAA 3 cut(s) 81, 195, 498
SatI GCNGC 2 cut(s) 347, 549
Sau3AI GATC 5 cut(s) 190, 232, 273, 451, 649
Sau96I GGNCC 2 cut(s) 418, 587
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 1 cut(s) 434
SfaNI GCATC 3 cut(s) 61, 533, 586
SfuI TTCGAA 1 cut(s) 151
SinI GGWCC 1 cut(s) 587
SmiMI CAYNNNNRTG 2 cut(s) 580, 610
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SphI GCATGC 1 cut(s) 555
Sse9I AATT 2 cut(s) 196, 446
SsiI CCGC 2 cut(s) 439, 593
SspMI CTAG 2 cut(s) 561, 687
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 1 cut(s) 90
TaiI ACGT 2 cut(s) 248, 437
TaqI TCGA 3 cut(s) 151, 628, 652
TasI AATT 2 cut(s) 196, 446
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 3 cut(s) 81, 195, 498
Tru9I TTAA 3 cut(s) 81, 195, 498
TscAI CASTG 3 cut(s) 49, 256, 537
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 346, 548
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 4 cut(s) 17, 284, 398, 486
TspGWI ACGGA 2 cut(s) 302, 393
TspRI CASTG 3 cut(s) 49, 256, 537
Van91I CCANNNNNTGG 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 587
XceI RCATGY 1 cut(s) 555
XcmI CCANNNNNNNNNTGG 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 245
XspI CTAG 2 cut(s) 561, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.