pycom05g32180
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010763
Physical Location & Seq
Forward (+)
31702148 .. 31704253
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
ATGATTTCTTTAGCACAAGACCATGAATTAACCCCCGACATCCTCAAAACTACAGTTGATTGGGAAAACATTAAACCTCCAGTTATAGAAGGTTTGGATGATTTGGTAGCTACTAGTAGTGATTTTGATTCTAAACCTATGGTCATGCTTTTGGGTCAGTATTCAACTGGGAAAACAACATTTATTAAACATTTGTTACGATGCAACTATCCAGGAGCTCACATTGGACCAGAACCTACTACAGATAGATTCGTTGTAGTGATGTCTGGACCTGATGAGAGGAGTATTCCCGGAAATACTATAGTTGTTCATGCAGACATGCCTTTCAGTGGTCTAACAACTTTCGGAGGTGCATTTTTGTCAAAATTTGAGTGTTCACAAATGCCACATCCAGTGAGTTTTGAAATTTTTGTAATCTTCTATTTATATAGCATTTCTTTGCTAGATGAAATTACATTTGTAGACACCCCTGGAGTTCTATCTGGAGAACAGCAAACAACAAATTTTGAACATGGTTATCCTAGCTTTGCAGTTTCTGTTGGATTTTTATTTCGAGGAAGACCTGTTGCTGCTGCCGTGATAGAGTTCGTTGGGGGCCCCATGTGTTGGAATACCTGCATTTTTTCTGCTAGTGCTGTTATGGAAGTTCTGCTTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005886 GO:0005911 GO:0005929 GO:0006810 GO:0006886 GO:0006897 GO:0006996 GO:0007275 GO:0007399 GO:0008104 GO:0008150 GO:0009506 GO:0009719 GO:0009987 GO:0010008 GO:0010033 GO:0010830 GO:0010831 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0020016 GO:0020018 GO:0022008 GO:0022603 GO:0022607 GO:0030030 GO:0030031 GO:0030054 GO:0030154 GO:0030182 GO:0031090 GO:0031175 GO:0031253 GO:0031410 GO:0031901 GO:0031982 GO:0032386 GO:0032388 GO:0032456 GO:0032501 GO:0032502 GO:0032879 GO:0032956 GO:0032970 GO:0033036 GO:0033043 GO:0033365 GO:0034613 GO:0042221 GO:0042886 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044441 GO:0044444 GO:0044446 GO:0044459 GO:0044463 GO:0044464 GO:0044782 GO:0045184 GO:0045595 GO:0045597 GO:0046907 GO:0048468 GO:0048518 GO:0048522 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051147 GO:0051149 GO:0051153 GO:0051155 GO:0051179 GO:0051234 GO:0051259 GO:0051260 GO:0051493 GO:0051641 GO:0051649 GO:0051716 GO:0055037 GO:0055038 GO:0055044 GO:0060142 GO:0060143 GO:0060170 GO:0060271 GO:0060341 GO:0060627 GO:0061512 GO:0065003 GO:0065007 GO:0070727 GO:0070848 GO:0070887 GO:0070925 GO:0071310 GO:0071363 GO:0071495 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0097708 GO:0098588 GO:0098590 GO:0098657 GO:0098805 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:1901739 GO:1901741 GO:1990089 GO:1990090 GO:2001135 GO:2001137
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Dynamin_N EF-hand_4 EF-hand_7 EHD_N

Protein Analysis

220

Amino Acids

24.01

Weight (kDa)

4.72

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dynamin_N PF00350 48 - 168 4.5e-11 Dynamin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 623
AccB7I CCANNNNNTGG 1 cut(s) 606
AccI GTMKAC 1 cut(s) 462
AcsI RAATTY 3 cut(s) 365, 405, 502
AfiI CCNNNNNNNGG 2 cut(s) 329, 606
AgsI TTSAA 3 cut(s) 165, 404, 509
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 2 cut(s) 211, 469
AluBI AGCT 3 cut(s) 110, 218, 525
AluI AGCT 3 cut(s) 110, 218, 525
Alw21I GWGCWC 1 cut(s) 220
AlwNI CAGNNNCTG 1 cut(s) 536
AoxI GGCC 1 cut(s) 595
ApaI GGGCCC 1 cut(s) 599
ApeKI GCWGC 2 cut(s) 569, 572
ApoI RAATTY 3 cut(s) 365, 405, 502
AspS9I GGNCC 4 cut(s) 227, 269, 595, 596
AsuC2I CCSGG 1 cut(s) 291
AvaII GGWCC 2 cut(s) 227, 269
BaeGI GKGCMC 1 cut(s) 599
BanII GRGCYC 2 cut(s) 220, 599
BbsI GAAGAC 1 cut(s) 565
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 556, 559
BceAI ACGGC 1 cut(s) 560
BciT130I CCWGG 2 cut(s) 213, 471
BcnI CCSGG 1 cut(s) 291
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 4 cut(s) 114, 443, 522, 630
BfmI CTRYAG 3 cut(s) 51, 240, 300
BfuAI ACCTGC 1 cut(s) 623
BisI GCNGC 2 cut(s) 570, 573
BlsI GCNGC 2 cut(s) 571, 574
Bme1390I CCNGG 3 cut(s) 213, 291, 471
Bme18I GGWCC 2 cut(s) 227, 269
BmgT120I GGNCC 4 cut(s) 227, 269, 595, 596
BmiI GGNNCC 3 cut(s) 596, 597, 598
BmrFI CCNGG 3 cut(s) 213, 291, 471
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 191
BmuI ACTGGG 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 565
BpmI CTGGAG 3 cut(s) 63, 492, 504
BpuMI CCSGG 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 469
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 606
Bse1I ACTGG 3 cut(s) 80, 172, 392
BseBI CCWGG 2 cut(s) 213, 471
BseDI CCNNGG 1 cut(s) 469
BseGI GGATG 3 cut(s) 39, 103, 388
BseLI CCNNNNNNNGG 2 cut(s) 329, 606
BseNI ACTGG 3 cut(s) 80, 172, 392
BseRI GAGGAG 1 cut(s) 295
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 2 cut(s) 556, 559
BshFI GGCC 1 cut(s) 597
BsiHKAI GWGCWC 1 cut(s) 220
BsiSI CCGG 1 cut(s) 291
BslI CCNNNNNNNGG 2 cut(s) 329, 606
BsnI GGCC 1 cut(s) 597
Bsp120I GGGCCC 1 cut(s) 595
Bsp1286I GDGCHC 2 cut(s) 220, 599
BspANI GGCC 1 cut(s) 597
BspLI GGNNCC 3 cut(s) 596, 597, 598
BspMI ACCTGC 1 cut(s) 623
BsrI ACTGG 3 cut(s) 80, 172, 392
BssECI CCNNGG 1 cut(s) 469
Bst2UI CCWGG 2 cut(s) 213, 471
Bst4CI ACNGT 1 cut(s) 55
BstF5I GGATG 3 cut(s) 39, 103, 388
BstNI CCWGG 2 cut(s) 213, 471
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 3 cut(s) 211, 289, 469
BstSFI CTRYAG 3 cut(s) 51, 240, 300
BstSLI GKGCMC 1 cut(s) 599
BstV1I GCAGC 2 cut(s) 556, 559
BstV2I GAAGAC 1 cut(s) 565
BsuRI GGCC 1 cut(s) 597
BtsCI GGATG 3 cut(s) 39, 103, 388
BtsIMutI CAGTG 2 cut(s) 334, 399
BveI ACCTGC 1 cut(s) 623
CaiI CAGNNNCTG 1 cut(s) 536
Cfr13I GGNCC 4 cut(s) 227, 269, 595, 596
CviAII CATG 6 cut(s) 23, 145, 311, 319, 512, 601
CviJI RGCY 4 cut(s) 110, 218, 525, 597
CviKI_1 RGCY 4 cut(s) 110, 218, 525, 597
Ecl136II GAGCTC 1 cut(s) 218
Eco24I GRGCYC 2 cut(s) 220, 599
Eco47I GGWCC 2 cut(s) 227, 269
Eco53kI GAGCTC 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 218
EcoO109I RGGNCCY 2 cut(s) 595, 596
EcoRII CCWGG 2 cut(s) 211, 469
EcoT38I GRGCYC 2 cut(s) 220, 599
FaeI CATG 6 cut(s) 26, 148, 314, 322, 515, 604
FalI AAGNNNNNCTT 1 cut(s) 636
FatI CATG 6 cut(s) 22, 144, 310, 318, 511, 600
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 2 cut(s) 570, 573
FokI GGATG 3 cut(s) 26, 110, 375
FriOI GRGCYC 2 cut(s) 220, 599
Fsp4HI GCNGC 2 cut(s) 570, 573
FspBI CTAG 4 cut(s) 114, 443, 522, 630
GluI GCNGC 2 cut(s) 570, 573
GsuI CTGGAG 3 cut(s) 63, 492, 504
HaeIII GGCC 1 cut(s) 597
HapII CCGG 1 cut(s) 291
Hin1II CATG 6 cut(s) 26, 148, 314, 322, 515, 604
HinfI GANTC 2 cut(s) 128, 249
HpaII CCGG 1 cut(s) 291
Hpy166II GTNNAC 2 cut(s) 377, 463
Hpy188I TCNGA 1 cut(s) 347
Hpy188III TCNNGA 2 cut(s) 267, 483
Hpy8I GTNNAC 2 cut(s) 377, 463
HpyAV CCTTC 1 cut(s) 83
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4V TGCA 5 cut(s) 204, 314, 353, 530, 618
Hsp92II CATG 6 cut(s) 26, 148, 314, 322, 515, 604
LmnI GCTCC 1 cut(s) 215
Lsp1109I GCAGC 2 cut(s) 556, 559
LweI GCATC 1 cut(s) 191
MaeI CTAG 4 cut(s) 114, 443, 522, 630
MaeIII GTNAC 1 cut(s) 195
MboII GAAGA 2 cut(s) 409, 570
MhlI GDGCHC 2 cut(s) 220, 599
MluCI AATT 5 cut(s) 26, 365, 405, 450, 502
MmeI TCCRAC 2 cut(s) 520, 587
MnlI CCTC 5 cut(s) 53, 87, 273, 341, 548
MseI TTAA 4 cut(s) 29, 72, 186, 654
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 3 cut(s) 213, 291, 471
MvaI CCWGG 2 cut(s) 213, 471
NciI CCSGG 1 cut(s) 291
NlaIII CATG 6 cut(s) 26, 148, 314, 322, 515, 604
NlaIV GGNNCC 3 cut(s) 596, 597, 598
NspI RCATGY 1 cut(s) 322
PfeI GAWTC 2 cut(s) 128, 249
PflMI CCANNNNNTGG 1 cut(s) 606
PfoI TCCNGGA 2 cut(s) 211, 289
PkrI GCNGC 2 cut(s) 571, 574
Psp124BI GAGCTC 1 cut(s) 220
Psp6I CCWGG 2 cut(s) 211, 469
PspGI CCWGG 2 cut(s) 211, 469
PspN4I GGNNCC 3 cut(s) 596, 597, 598
PspOMI GGGCCC 1 cut(s) 595
PspPI GGNCC 4 cut(s) 227, 269, 595, 596
PstNI CAGNNNCTG 1 cut(s) 536
SacI GAGCTC 1 cut(s) 220
SaqAI TTAA 4 cut(s) 29, 72, 186, 654
SatI GCNGC 2 cut(s) 570, 573
Sau96I GGNCC 4 cut(s) 227, 269, 595, 596
ScrFI CCNGG 3 cut(s) 213, 291, 471
SduI GDGCHC 2 cut(s) 220, 599
SfaNI GCATC 1 cut(s) 191
SfcI CTRYAG 3 cut(s) 51, 240, 300
SinI GGWCC 2 cut(s) 227, 269
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 5 cut(s) 26, 365, 405, 450, 502
SspMI CTAG 4 cut(s) 114, 443, 522, 630
SstI GAGCTC 1 cut(s) 220
StyD4I CCNGG 3 cut(s) 211, 289, 469
TaaI ACNGT 1 cut(s) 55
TaqI TCGA 1 cut(s) 553
TasI AATT 5 cut(s) 26, 365, 405, 450, 502
TfiI GAWTC 2 cut(s) 128, 249
Tru1I TTAA 4 cut(s) 29, 72, 186, 654
Tru9I TTAA 4 cut(s) 29, 72, 186, 654
TscAI CASTG 2 cut(s) 334, 399
TseI GCWGC 2 cut(s) 569, 572
TspDTI ATGAA 3 cut(s) 39, 299, 462
TspRI CASTG 2 cut(s) 334, 399
Van91I CCANNNNNTGG 1 cut(s) 606
VpaK11BI GGWCC 2 cut(s) 227, 269
XapI RAATTY 3 cut(s) 365, 405, 502
XceI RCATGY 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 462
XspI CTAG 4 cut(s) 114, 443, 522, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.