Rh5BG017100
ERF Family

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin Fzo YdjA family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010763
Physical Location & Seq
Reverse (-)
1229960 .. 1234299
4340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG017100.1

Sequence Viewer

Length: 1311 bp
ATGGAGATCGGGTCGGGTCCGATCGGGTCGTGCTCGAAAGAGCAGCAGAAGATTTACAGGGACTGGTTCAACTTTGTCGATTCAGATGGAGATGGAAGGATTACTGGTAACGAAGGCGTGAAGTTCTTCGCCATGTCCAAGCTTTCTAGGCCCGAACTCAAGCAGGTTTGGGCGATTGCTGATTCGAAACGACAGGGTTTTTTAGGGTTTACCGAGTTCATCACAGCAATGCAGCTGATTTCCTTTGCGCAAGATCATGAATTAAGTCCAGACATCCTCAAAGCTGAAGTTGACTGGGAGAACATTAAACCTCCAGTGATCGAAGGTGTGGATGCTTTAATAGCTAAAACTAAGAGTTCTACAACAAATGGAGTTGCCCTGAATGGAAATGGAATCACTCCTACTCAACCATCAGCTGAACGGACTACTTCAAAATCAGTTAAGAAACTACCTCTCAATGCAGTTACATCTATAAGTGATGGCTTGAAGAAATTGTACATTGAAAAACTAAAACCACTGGAAGCTGCATATCGTTTCAATGATTTTGGTAATCCATTACTGACTAACAGTGATTTTGATGCCAAGCCTATGGTCATGCTTTTGGGTCAGTATTCAACTGGAAAAACAACATTTATAAAGCATTTGTTAAGATGTAACTATCCAGGAGCTCATATTGGGCCAGAGCCTACAACTGATAGATTTGTTGTTGTGATGTCTGGACCTGATGAAAGGAGCATTCCTGGAAATACCATAGCTGTTCATGCAGACATGCCTTTTAGCGGTCTAACAAGTTTTGGAGGTTCATTTCTGTCAAAATTTGAGTGTTCCCAAATGCCTCATCCATTGCTAGATCAAATTACACTTGTAGACACTCCTGGGGTTCTATCTGGAGAAAAGCAACGAACGCAAAGGAGTTATGATTTCACTGGTGTCATATCGTGGTTTGCTGCAAAATGTGATCTCATCCTTCTTCTTTTCGACCCTCATAAGCTTGATATCAGTGATGAATTTAAGCGTGTAATTGCATCTCTACGTGGGCATGATGATAAGATTCGAGTGGTTCTAAACAAAGCAGATCAAGTTGATACTCAACAACTGATGAGAGTTTATGGGGCATTAATGTGGTCACTTGGAAAGGTTTTGAATACTCCAGAGGTTGTGCGTGTTTATATTGGCTCATTTAATGATAAACCTGTCAATGAAGAAGCTGTCGGCCCCATGGGTAGAGAGCTTTTTGAAATGGAACAAGGTGACCTCCTTGCTGACCTGGTTGATATACCAAAGAAAGCTTGTGATCGCCGGGTAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005886 GO:0005911 GO:0005929 GO:0006810 GO:0006886 GO:0006897 GO:0006996 GO:0007275 GO:0007399 GO:0008104 GO:0008150 GO:0009506 GO:0009719 GO:0009987 GO:0010008 GO:0010033 GO:0010830 GO:0010831 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0020016 GO:0020018 GO:0022008 GO:0022603 GO:0022607 GO:0030030 GO:0030031 GO:0030054 GO:0030154 GO:0030182 GO:0031090 GO:0031175 GO:0031253 GO:0031410 GO:0031901 GO:0031982 GO:0032386 GO:0032388 GO:0032456 GO:0032501 GO:0032502 GO:0032879 GO:0032956 GO:0032970 GO:0033036 GO:0033043 GO:0033365 GO:0034613 GO:0042221 GO:0042886 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044441 GO:0044444 GO:0044446 GO:0044459 GO:0044463 GO:0044464 GO:0044782 GO:0045184 GO:0045595 GO:0045597 GO:0046907 GO:0048468 GO:0048518 GO:0048522 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051128 GO:0051130 GO:0051147 GO:0051149 GO:0051153 GO:0051155 GO:0051179 GO:0051234 GO:0051259 GO:0051260 GO:0051493 GO:0051641 GO:0051649 GO:0051716 GO:0055037 GO:0055038 GO:0055044 GO:0060142 GO:0060143 GO:0060170 GO:0060271 GO:0060341 GO:0060627 GO:0061512 GO:0065003 GO:0065007 GO:0070727 GO:0070848 GO:0070887 GO:0070925 GO:0071310 GO:0071363 GO:0071495 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0097708 GO:0098588 GO:0098590 GO:0098657 GO:0098805 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:1901739 GO:1901741 GO:1990089 GO:1990090 GO:2001135 GO:2001137
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Dynamin_N EF-hand_4 EF-hand_7 EHD_N

Protein Analysis

436

Amino Acids

48.33

Weight (kDa)

6.05

Isoelectric Point (pI)

26.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EH PF12763 14 - 87 6e-10 EH domain
EF-hand_7 PF13499 23 - 78 1.4e-06 EF-hand domain pair
EHD_N PF16880 161 - 193 4.6e-15 N-terminal EH-domain containing protein
Dynamin_N PF00350 198 - 358 1.2e-13 Dynamin family
MMR_HSR1 PF01926 198 - 357 2.4e-07 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
Acc16I TGCGCA 1 cut(s) 249
Acc36I ACCTGC 1 cut(s) 154
AccI GTMKAC 1 cut(s) 867
AciI CCGC 1 cut(s) 780
AcsI RAATTY 2 cut(s) 815, 1007
AcuI CTGAAG 1 cut(s) 306
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 1 cut(s) 779
AgsI TTSAA 8 cut(s) 70, 432, 487, 503, 538, 615, 1144, 1238
AjnI CCWGG 4 cut(s) 661, 739, 874, 1266
Alw21I GWGCWC 2 cut(s) 35, 670
AlwNI CAGNNNCTG 1 cut(s) 63
AoxI GGCC 3 cut(s) 149, 677, 1213
ApeKI GCWGC 4 cut(s) 43, 232, 524, 947
ApoI RAATTY 2 cut(s) 815, 1007
AseI ATTAAT 1 cut(s) 1118
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 5 cut(s) 17, 150, 677, 719, 1214
AsuC2I CCSGG 1 cut(s) 1301
AsuHPI GGTGA 1 cut(s) 1262
AsuII TTCGAA 1 cut(s) 185
AvaII GGWCC 2 cut(s) 17, 719
BanII GRGCYC 1 cut(s) 670
BarI GAAGNNNNNNTAC 2 cut(s) 479, 511
Bbv12I GWGCWC 2 cut(s) 35, 670
BbvI GCAGC 4 cut(s) 55, 244, 511, 934
BccI CCATC 4 cut(s) 80, 86, 418, 473
BciT130I CCWGG 4 cut(s) 663, 741, 876, 1268
BcnI CCSGG 1 cut(s) 1301
BfaI CTAG 2 cut(s) 147, 848
BfuAI ACCTGC 1 cut(s) 154
BisI GCNGC 4 cut(s) 44, 233, 525, 948
BlsI GCNGC 4 cut(s) 45, 234, 526, 949
Bme1390I CCNGG 5 cut(s) 663, 741, 876, 1268, 1301
Bme18I GGWCC 2 cut(s) 17, 719
BmgT120I GGNCC 5 cut(s) 17, 150, 677, 719, 1214
BmiI GGNNCC 2 cut(s) 18, 1216
BmrFI CCNGG 5 cut(s) 663, 741, 876, 1268, 1301
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 3 cut(s) 322, 568, 1034
BmuI ACTGGG 1 cut(s) 304
BpmI CTGGAG 3 cut(s) 297, 909, 1134
Bpu14I TTCGAA 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 1301
BsaAI YACGTR 1 cut(s) 1034
BsaJI CCNNGG 2 cut(s) 875, 1218
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 779
Bse1I ACTGG 7 cut(s) 68, 109, 299, 314, 522, 622, 931
Bse3DI GCAATG 2 cut(s) 234, 842
BseBI CCWGG 4 cut(s) 663, 741, 876, 1268
BseDI CCNNGG 2 cut(s) 875, 1218
BseGI GGATG 4 cut(s) 273, 337, 838, 963
BseLI CCNNNNNNNGG 1 cut(s) 779
BseMI GCAATG 2 cut(s) 234, 842
BseNI ACTGG 7 cut(s) 68, 109, 299, 314, 522, 622, 931
BseXI GCAGC 4 cut(s) 55, 244, 511, 934
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 3 cut(s) 151, 679, 1215
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 2 cut(s) 35, 670
BsiSI CCGG 1 cut(s) 1300
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 74
BsmI GAATGC 1 cut(s) 735
BsnI GGCC 3 cut(s) 151, 679, 1215
Bsp119I TTCGAA 1 cut(s) 185
Bsp1286I GDGCHC 2 cut(s) 35, 670
Bsp1407I TGTACA 1 cut(s) 495
Bsp143I GATC 8 cut(s) 6, 21, 253, 318, 850, 958, 1075, 1294
Bsp19I CCATGG 1 cut(s) 1218
BspACI CCGC 1 cut(s) 780
BspANI GGCC 3 cut(s) 151, 679, 1215
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 2 cut(s) 18, 1216
BspMI ACCTGC 1 cut(s) 154
BspT104I TTCGAA 1 cut(s) 185
BsrDI GCAATG 2 cut(s) 234, 842
BsrGI TGTACA 1 cut(s) 495
BsrI ACTGG 7 cut(s) 68, 109, 299, 314, 522, 622, 931
BssECI CCNNGG 2 cut(s) 875, 1218
BssMI GATC 8 cut(s) 6, 21, 253, 318, 850, 958, 1075, 1294
BssT1I CCWWGG 1 cut(s) 1218
Bst2UI CCWGG 4 cut(s) 663, 741, 876, 1268
Bst4CI ACNGT 1 cut(s) 569
BstAUI TGTACA 1 cut(s) 495
BstBAI YACGTR 1 cut(s) 1034
BstBI TTCGAA 1 cut(s) 185
BstDEI CTNAG 1 cut(s) 351
BstDSI CCRYGG 1 cut(s) 1218
BstEII GGTNACC 1 cut(s) 1250
BstF5I GGATG 4 cut(s) 273, 337, 838, 963
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 8 cut(s) 9, 24, 256, 321, 853, 961, 1078, 1297
BstMBI GATC 8 cut(s) 6, 21, 253, 318, 850, 958, 1075, 1294
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 4 cut(s) 148, 341, 761, 904
BstNI CCWGG 4 cut(s) 663, 741, 876, 1268
BstNSI RCATGY 1 cut(s) 772
BstPI GGTNACC 1 cut(s) 1250
BstSCI CCNGG 5 cut(s) 661, 739, 874, 1266, 1299
BstV1I GCAGC 4 cut(s) 55, 244, 511, 934
BstXI CCANNNNNNTGG 1 cut(s) 589
BsuRI GGCC 3 cut(s) 151, 679, 1215
BtgI CCRYGG 1 cut(s) 1218
BtsCI GGATG 4 cut(s) 273, 337, 838, 963
BtsIMutI CAGTG 5 cut(s) 321, 515, 574, 924, 1006
BveI ACCTGC 1 cut(s) 154
CaiI CAGNNNCTG 1 cut(s) 63
CciI TCATGA 1 cut(s) 256
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 5 cut(s) 17, 150, 677, 719, 1214
CsiI ACCWGGT 1 cut(s) 1266
Csp6I GTAC 1 cut(s) 496
CviAII CATG 7 cut(s) 133, 257, 595, 761, 769, 1040, 1219
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 1 cut(s) 351
DpnI GATC 8 cut(s) 8, 23, 255, 320, 852, 960, 1077, 1296
DpnII GATC 8 cut(s) 6, 21, 253, 318, 850, 958, 1075, 1294
Ecl136II GAGCTC 1 cut(s) 668
Eco130I CCWWGG 1 cut(s) 1218
Eco24I GRGCYC 1 cut(s) 670
Eco32I GATATC 1 cut(s) 997
Eco47I GGWCC 2 cut(s) 17, 719
Eco53kI GAGCTC 1 cut(s) 668
Eco57I CTGAAG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 1250
EcoICRI GAGCTC 1 cut(s) 668
EcoO65I GGTNACC 1 cut(s) 1250
EcoRII CCWGG 4 cut(s) 661, 739, 874, 1266
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 1 cut(s) 1218
EcoT38I GRGCYC 1 cut(s) 670
ErhI CCWWGG 1 cut(s) 1218
FaeI CATG 7 cut(s) 136, 260, 598, 764, 772, 1043, 1222
FaqI GGGAC 1 cut(s) 74
FatI CATG 7 cut(s) 132, 256, 594, 760, 768, 1039, 1218
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 4 cut(s) 44, 233, 525, 948
FokI GGATG 4 cut(s) 260, 344, 825, 950
FriOI GRGCYC 1 cut(s) 670
Fsp4HI GCNGC 4 cut(s) 44, 233, 525, 948
FspBI CTAG 2 cut(s) 147, 848
FspI TGCGCA 1 cut(s) 249
GlaI GCGC 1 cut(s) 249
GluI GCNGC 4 cut(s) 44, 233, 525, 948
GsuI CTGGAG 3 cut(s) 297, 909, 1134
HaeIII GGCC 3 cut(s) 151, 679, 1215
HapII CCGG 1 cut(s) 1300
HhaI GCGC 1 cut(s) 250
Hin1II CATG 7 cut(s) 136, 260, 598, 764, 772, 1043, 1222
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HindIII AAGCTT 3 cut(s) 140, 989, 1287
HinfI GANTC 4 cut(s) 80, 182, 393, 1051
HpaII CCGG 1 cut(s) 1300
HphI GGTGA 1 cut(s) 1262
Hpy166II GTNNAC 3 cut(s) 210, 292, 868
Hpy188I TCNGA 2 cut(s) 21, 85
Hpy188III TCNNGA 5 cut(s) 257, 269, 717, 888, 1151
Hpy8I GTNNAC 3 cut(s) 210, 292, 868
HpyAV CCTTC 4 cut(s) 90, 107, 317, 977
HpyCH4III ACNGT 1 cut(s) 569
HpyCH4IV ACGT 1 cut(s) 1033
HpyCH4V TGCA 6 cut(s) 232, 461, 527, 764, 950, 1025
HpyF10VI GCNNNNNNNGC 4 cut(s) 148, 341, 761, 904
HpyF3I CTNAG 1 cut(s) 351
HpySE526I ACGT 1 cut(s) 1033
Hsp92II CATG 7 cut(s) 136, 260, 598, 764, 772, 1043, 1222
HspAI GCGC 1 cut(s) 248
Kzo9I GATC 8 cut(s) 6, 21, 253, 318, 850, 958, 1075, 1294
LmnI GCTCC 2 cut(s) 665, 732
Lsp1109I GCAGC 4 cut(s) 55, 244, 511, 934
LweI GCATC 3 cut(s) 322, 568, 1034
MabI ACCWGGT 1 cut(s) 1266
MaeI CTAG 2 cut(s) 147, 848
MaeII ACGT 1 cut(s) 1033
MaeIII GTNAC 5 cut(s) 107, 463, 653, 1125, 1250
MalI GATC 8 cut(s) 8, 23, 255, 320, 852, 960, 1077, 1296
MboI GATC 8 cut(s) 6, 21, 253, 318, 850, 958, 1075, 1294
MboII GAAGA 5 cut(s) 61, 118, 499, 962, 1214
MhlI GDGCHC 2 cut(s) 35, 670
MluCI AATT 6 cut(s) 260, 491, 815, 855, 1007, 1020
MnlI CCTC 8 cut(s) 287, 321, 462, 791, 846, 993, 1147, 1265
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 8 cut(s) 263, 306, 338, 441, 647, 1011, 1118, 1182
MslI CAYNNNNRTG 2 cut(s) 227, 1120
MspA1I CMGCKG 2 cut(s) 235, 416
MspI CCGG 1 cut(s) 1300
MspR9I CCNGG 5 cut(s) 663, 741, 876, 1268, 1301
Mva1269I GAATGC 1 cut(s) 735
MvaI CCWGG 4 cut(s) 663, 741, 876, 1268
MwoI GCNNNNNNNGC 4 cut(s) 148, 341, 761, 904
NciI CCSGG 1 cut(s) 1301
NcoI CCATGG 1 cut(s) 1218
NdeII GATC 8 cut(s) 6, 21, 253, 318, 850, 958, 1075, 1294
NlaIII CATG 7 cut(s) 136, 260, 598, 764, 772, 1043, 1222
NlaIV GGNNCC 2 cut(s) 18, 1216
NmuCI GTSAC 2 cut(s) 1125, 1250
NsbI TGCGCA 1 cut(s) 249
NspI RCATGY 1 cut(s) 772
NspV TTCGAA 1 cut(s) 185
PagI TCATGA 1 cut(s) 256
PctI GAATGC 1 cut(s) 735
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 4 cut(s) 80, 182, 393, 1051
PfoI TCCNGGA 2 cut(s) 661, 739
PkrI GCNGC 4 cut(s) 45, 234, 526, 949
Ple19I CGATCG 1 cut(s) 24
Ppu21I YACGTR 1 cut(s) 1034
PshBI ATTAAT 1 cut(s) 1118
PsiI TTATAA 1 cut(s) 635
Psp124BI GAGCTC 1 cut(s) 670
Psp6I CCWGG 4 cut(s) 661, 739, 874, 1266
PspEI GGTNACC 1 cut(s) 1250
PspGI CCWGG 4 cut(s) 661, 739, 874, 1266
PspN4I GGNNCC 2 cut(s) 18, 1216
PspPI GGNCC 5 cut(s) 17, 150, 677, 719, 1214
PstNI CAGNNNCTG 1 cut(s) 63
PvuI CGATCG 1 cut(s) 24
PvuII CAGCTG 2 cut(s) 235, 416
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 2 cut(s) 227, 1120
SacI GAGCTC 1 cut(s) 670
SaqAI TTAA 8 cut(s) 263, 306, 338, 441, 647, 1011, 1118, 1182
SatI GCNGC 4 cut(s) 44, 233, 525, 948
Sau3AI GATC 8 cut(s) 6, 21, 253, 318, 850, 958, 1075, 1294
Sau96I GGNCC 5 cut(s) 17, 150, 677, 719, 1214
ScrFI CCNGG 5 cut(s) 663, 741, 876, 1268, 1301
SduI GDGCHC 2 cut(s) 35, 670
SexAI ACCWGGT 1 cut(s) 1266
SfaNI GCATC 3 cut(s) 322, 568, 1034
SfuI TTCGAA 1 cut(s) 185
SinI GGWCC 2 cut(s) 17, 719
SmiMI CAYNNNNRTG 2 cut(s) 227, 1120
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
Sse9I AATT 6 cut(s) 260, 491, 815, 855, 1007, 1020
SsiI CCGC 1 cut(s) 780
SspMI CTAG 2 cut(s) 147, 848
SstI GAGCTC 1 cut(s) 670
StyD4I CCNGG 5 cut(s) 661, 739, 874, 1266, 1299
StyI CCWWGG 1 cut(s) 1218
TaaI ACNGT 1 cut(s) 569
TaiI ACGT 1 cut(s) 1036
TaqI TCGA 6 cut(s) 35, 78, 185, 321, 978, 1054
TasI AATT 6 cut(s) 260, 491, 815, 855, 1007, 1020
TatI WGTACW 1 cut(s) 495
TfiI GAWTC 4 cut(s) 80, 182, 393, 1051
Tru1I TTAA 8 cut(s) 263, 306, 338, 441, 647, 1011, 1118, 1182
Tru9I TTAA 8 cut(s) 263, 306, 338, 441, 647, 1011, 1118, 1182
TscAI CASTG 5 cut(s) 321, 522, 574, 931, 1006
TseFI GTSAC 2 cut(s) 1125, 1250
TseI GCWGC 4 cut(s) 43, 232, 524, 947
Tsp45I GTSAC 2 cut(s) 1125, 1250
TspDTI ATGAA 7 cut(s) 208, 273, 741, 749, 792, 1020, 1215
TspGWI ACGGA 1 cut(s) 436
TspRI CASTG 5 cut(s) 321, 522, 574, 931, 1006
VpaK11BI GGWCC 2 cut(s) 17, 719
VspI ATTAAT 1 cut(s) 1118
XapI RAATTY 2 cut(s) 815, 1007
XceI RCATGY 1 cut(s) 772
XmiI GTMKAC 1 cut(s) 867
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 2 cut(s) 147, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.