pycom06g00850

Protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0011315
Physical Location & Seq
Reverse (-)
805068 .. 811438
6371 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2754 bp
ATGGATGTTGAAGTGATTGATGTCGAAGGAATGGTGCATCATGGAATAGCGGATGATGGTGATGCTGAACGAAGTGAAGGTGGAGAAGTGAATAACACGGAAAATTCCGAGGCTCATGGTGAAGATGGGATTTCCGAGCCATATGTGGGTATGGAATTTACTTCTGAAGAGGCTGCCAAGACTTTCTATGATGAATACGCAAGGCGTTTGGGTTTTAGCTCTAAAGTTGGTCAATCTAGTCGTTCTAAACCTGATGCGACAACCATTGCTCGGGAGTTTGTATGTGGCAGAGAGGGTTTGAAAAGAAGGCAGGCTGATAGTTGTGGTGCAATGCTTAGGATAGAGTTAAAGCGTCAAGATAAGTGGGTTTTGACGAAATTTGTGAAGGAGCATAGTCATGCATTAGCGAGTCCTGGTAAAATGCAATACCTTCGGCCTCGTCGGCATTTTGCTGGTGCTGCAAAGAATGCAGCTGAAACCTATCAAGGAGTAGGGATTGTTCCCAGTGGTGTTATGTATCTTTCCATGGATGGAAACCATGGTAACCGTGCCATTGTAGAGAAAAACCGTGTAGTTAGGAATACTTCATCTGCAGAATCAAATAGACCAGTCAAGAATGCTGTAACAATCAATCATGCTCTTAGGCCTACTAGTCGAAGGAGGACACTAGGGAAGGATGCTCAGAATTTGCTGGAGTATTTCAAGAAAATGCAGGCTGAGAACCCTGGTTTCTTTTATGCAATACAGCTTGATGAAGATAATCACATGGCAAACGTGTTCTGGGCAGATGTGAGGTCAAGAACTGCGTACTGTCATTTTGGTGATTTTGTGACTCTGGATACCACATACAGAGTAAATCAGTATAGAGTGCCATTTGCTCCATTTACCGGAGTGAACCATCATGGTCAAACGGTTTTGTTTGGTTGTGCATTACTTCTAGATGAGTCTGAAGCGTCTTTTATTTGGCTGTTCAAGACATTCCTTACAGCAATGAATGATAGCCCTCCTGTTTCGATTATGACTGATCAGGACAGAGCCATACAGACAGCAGTTGCTCAGGTGTTTCCAGAAGTCCGCCACTGCATTAGCAAGTGGCATGTTTTAAGAGAGGGTCAGGATAGGTTGGCACATGTGTGCCACGCACACCCCAATTTTCAGTTGGAACTTTATAATTGTATCAATTTGACTGAAACCGTCGAGGAGTTTGAATTGTCGTGGGATTCTATCGTTGATAAATATGACCTCAGGAGAAATGATTGGCTTCAATCATTATACAATGCTCGCGCTCAATGGGTACCTGTGTATTTTCGGGATTCCTTTTTTGCTGCTATATCTCCCAATCAAGGGTATGATGGCTCCTTTTTTGAAGGCTATGTGAATCAACAGACTACATTACCATTGTTCTTTAGGCAATATGAAAGAGCTTTGGAGAACTGGTTTGAAAGGGAAATAGAAGCAGACTTTGATACAATTTGCACGACACCAGTTTTGAGAACACCATCACCCATGGAGAAACAGGCAGCCAATCTTTATACAAGAAAAATTTTTGCAAAATTTCAGGAAGAGCTAGTTGAGACTTTTGTGTACACAGCAAATAGGATTGAAGGTGATGGAGCTATTAGCACATTCAGAGTTGCGAAATTTGAAGATGACAACAAGGCATACATCGTTACATTTAACTATCCGGAGTTGAGAGCTAATTGTAGTTGTCAAATGTTTGAGTATTCAGGCATTCTTTGTAGACATGTTTTGACAGTATTTACTGTAACAAATGTGCTTACATTGCCTTCCCATTATATTTTGAAACGATGGACAAGAAATGCAAAGAATGGAACTAACCCAGATGAACGTAGTGGTGAGTTACATGGCCAAGAGTCTCTAACTTTACGGTACAACAATCTATGTCGAGAAGCCATAAAGTATGCAGAGGATGGGGCAACCACTACAGAAACTTTTATTGCAGCAATGACTGCTCTTAAAGAGGGTGGGAAGAAGGTTTCTGTTGTGAAAAACAATGTTGCTAAAGTTGCCCCTCCTAGCGGTCAGATTAGTGTGACTGGCTATGATGACAAAAAAAACTCGACATCAACATCAGATTTGACTCCTTCGTTGTGGCCACGCCATGATGAAGTAATGAGGCGTTTTAATTTGAATGATGCTGGTGCACCTGCTCAAAATGTTTCTGACCTGAATTTGCCACGGATGGCCCCTGTATCTCTTCATCGGGATGATGGTACTCCTGAGAACATGGTGGTACTTCCGTGTCTAAAGTCAATGACTTGGGTCATGGAAAGTAAGAATTCAGTACCAGGAAATAGAGTAGCTGTCATTAACCTGAAGTTGCAAGATTATAGCAGAATTCCGTCAACTGAATCAGAGGTTAAGTTTCAGCTCTCAAGGGTTTCATTGGAGCCAATGTTAAGGTCCATGTCCTACATCAGTATCGATCAGCTATCAACACCAGCTAATAAGGCTGATAGGCTCATGGCAATGCCCTTGGAGTATCATTTTTCAATCCCTTACTATGTTTTGTTTGAAGCTCCAACTCACAGAGGGAAAGAGGGAGTGTTTCTGTTTTGGAAGGATTTATCGCTTCAAGACACAGAGACAACTTCAGGGGAGTCAGAGGTCAAGTTTCAAGTTTCTAGGGATACATTAGGTGCCATGCTAAGATCAATGGCCTATATCCGCGAGCAGCTTTCAAGTGCTGCTGAGATCCAGCCAGAGTCCGTCGCTAAAAAGCAGCGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COMM_domain FAR1 MULE SWIM

Protein Analysis

918

Amino Acids

104.05

Weight (kDa)

6.16

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 61 - 136 3.4e-16 FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like PF21056 237 - 359 7.9e-10 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 277 - 368 1.1e-27 MULE transposase domain
SWIM PF04434 564 - 589 7.5e-09 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1170
AarI CACCTGC 1 cut(s) 2176
Acc36I ACCTGC 1 cut(s) 2176
Acc65I GGTACC 1 cut(s) 1294
AccB1I GGYRCC 2 cut(s) 1294, 2660
AccI GTMKAC 1 cut(s) 1741
AccII CGCG 2 cut(s) 1284, 2691
AccIII TCCGGA 1 cut(s) 1684
AciI CCGC 5 cut(s) 50, 1075, 2040, 2689, 2746
AclWI GGATC 1 cut(s) 2710
AcoI YGGCCR 2 cut(s) 1867, 2114
AcuI CTGAAG 4 cut(s) 186, 969, 2357, 2598
AfaI GTAC 7 cut(s) 809, 1296, 1586, 1892, 2236, 2256, 2307
AfiI CCNNNNNNNGG 6 cut(s) 146, 270, 887, 1343, 1344, 2039
AflIII ACRYGT 3 cut(s) 774, 1129, 1744
AhlI ACTAGT 1 cut(s) 650
AjnI CCWGG 3 cut(s) 412, 724, 2308
AleI CACNNNNGTG 1 cut(s) 1132
Alw21I GWGCWC 1 cut(s) 2167
Alw26I GTCTC 3 cut(s) 1568, 1881, 2600
Alw44I GTGCAC 1 cut(s) 2163
AlwI GGATC 1 cut(s) 2710
Ama87I CYCGRG 1 cut(s) 270
Aor13HI TCCGGA 1 cut(s) 1684
AoxI GGCC 6 cut(s) 434, 644, 1867, 2114, 2205, 2679
ApaLI GTGCAC 1 cut(s) 2163
ApeKI GCWGC 9 cut(s) 173, 458, 470, 1325, 1520, 1961, 2695, 2708, 2743
ArsI GACNNNNNNTTYG 2 cut(s) 1471, 1503
Asp718I GGTACC 1 cut(s) 1294
AspLEI GCGC 1 cut(s) 1286
AspS9I GGNCC 2 cut(s) 2206, 2424
AsuHPI GGTGA 6 cut(s) 71, 131, 833, 1494, 1619, 1868
AvaI CYCGRG 1 cut(s) 270
AvaII GGWCC 1 cut(s) 2424
AxyI CCTNAGG 1 cut(s) 1244
BaeGI GKGCMC 1 cut(s) 2167
BaeI ACNNNNGTAYC 6 cut(s) 1286, 1319, 2246, 2279, 2425, 2458
BalI TGGCCA 2 cut(s) 1869, 2116
BanI GGYRCC 2 cut(s) 1294, 2660
Bbv12I GWGCWC 1 cut(s) 2167
BbvI GCAGC 8 cut(s) 160, 445, 482, 1312, 1532, 1973, 2695, 2707
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 3 cut(s) 414, 726, 2310
BciVI GTATCC 2 cut(s) 832, 2644
BclI TGATCA 1 cut(s) 1024
BcoDI GTCTC 3 cut(s) 1568, 1881, 2600
BcuI ACTAGT 1 cut(s) 650
BfaI CTAG 7 cut(s) 237, 651, 668, 938, 1568, 2037, 2646
BfmI CTRYAG 2 cut(s) 591, 1944
BfuAI ACCTGC 1 cut(s) 2176
BfuI GTATCC 2 cut(s) 832, 2644
BglI GCCNNNNNGGC 1 cut(s) 442
BisI GCNGC 9 cut(s) 174, 459, 471, 1326, 1521, 1962, 2696, 2709, 2744
BlsI GCNGC 9 cut(s) 175, 460, 472, 1327, 1522, 1963, 2697, 2710, 2745
Bme1390I CCNGG 3 cut(s) 414, 726, 2310
Bme18I GGWCC 1 cut(s) 2424
BmeT110I CYCGRG 1 cut(s) 270
BmgT120I GGNCC 2 cut(s) 2206, 2424
BmiI GGNNCC 5 cut(s) 1296, 1357, 2208, 2412, 2662
BmrFI CCNGG 3 cut(s) 414, 726, 2310
BmrI ACTGGG 1 cut(s) 498
BmsI GCATC 5 cut(s) 46, 52, 244, 667, 2146
BmuI ACTGGG 1 cut(s) 498
BoxI GACNNNNGTC 1 cut(s) 2282
BpmI CTGGAG 1 cut(s) 713
Bpu10I CCTNAGC 2 cut(s) 335, 1056
BpuEI CTTGAG 1 cut(s) 2380
Bsa29I ATCGAT 1 cut(s) 2445
BsaJI CCNNGG 7 cut(s) 108, 525, 538, 724, 1506, 2198, 2496
BsaWI WCCGGW 2 cut(s) 887, 1684
BsaXI ACNNNNNCTCC 4 cut(s) 1421, 1451, 2402, 2432
Bsc4I CCNNNNNNNGG 6 cut(s) 146, 270, 887, 1343, 1344, 2039
Bse1I ACTGG 5 cut(s) 504, 608, 1439, 1484, 2062
Bse21I CCTNAGG 1 cut(s) 1244
Bse3DI GCAATG 6 cut(s) 264, 336, 996, 1781, 1971, 2496
BseAI TCCGGA 1 cut(s) 1684
BseBI CCWGG 3 cut(s) 414, 726, 2310
BseCI ATCGAT 1 cut(s) 2445
BseDI CCNNGG 7 cut(s) 108, 525, 538, 724, 1506, 2198, 2496
BseGI GGATG 7 cut(s) 10, 58, 535, 682, 1936, 2208, 2233
BseLI CCNNNNNNNGG 6 cut(s) 146, 270, 887, 1343, 1344, 2039
BseMI GCAATG 6 cut(s) 264, 336, 996, 1781, 1971, 2496
BseMII CTCAG 6 cut(s) 695, 708, 1070, 1258, 2232, 2703
BseNI ACTGG 5 cut(s) 504, 608, 1439, 1484, 2062
BseRI GAGGAG 1 cut(s) 1214
BseSI GKGCMC 1 cut(s) 2167
BseXI GCAGC 8 cut(s) 160, 445, 482, 1312, 1532, 1973, 2695, 2707
Bsh1236I CGCG 2 cut(s) 1284, 2691
BshFI GGCC 6 cut(s) 436, 646, 1869, 2116, 2207, 2681
BshNI GGYRCC 2 cut(s) 1294, 2660
BshVI ATCGAT 1 cut(s) 2445
BsiHKAI GWGCWC 1 cut(s) 2167
BsiHKCI CYCGRG 1 cut(s) 270
BsiSI CCGG 2 cut(s) 888, 1685
BslI CCNNNNNNNGG 6 cut(s) 146, 270, 887, 1343, 1344, 2039
BsmAI GTCTC 3 cut(s) 1568, 1881, 2600
BsmI GAATGC 3 cut(s) 472, 622, 1731
BsnI GGCC 6 cut(s) 436, 646, 1869, 2116, 2207, 2681
BsoBI CYCGRG 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 2167
Bsp13I TCCGGA 1 cut(s) 1684
Bsp1407I TGTACA 1 cut(s) 1584
Bsp143I GATC 4 cut(s) 1024, 2446, 2672, 2715
Bsp19I CCATGG 3 cut(s) 525, 538, 1506
BspACI CCGC 5 cut(s) 50, 1075, 2040, 2689, 2746
BspANI GGCC 6 cut(s) 436, 646, 1869, 2116, 2207, 2681
BspCNI CTCAG 6 cut(s) 694, 709, 1069, 1257, 2233, 2704
BspDI ATCGAT 1 cut(s) 2445
BspEI TCCGGA 1 cut(s) 1684
BspFNI CGCG 2 cut(s) 1284, 2691
BspLI GGNNCC 5 cut(s) 1296, 1357, 2208, 2412, 2662
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 2176
BspPI GGATC 1 cut(s) 2710
BspQI GCTCTTC 1 cut(s) 1557
BspT107I GGYRCC 2 cut(s) 1294, 2660
BsrDI GCAATG 6 cut(s) 264, 336, 996, 1781, 1971, 2496
BsrGI TGTACA 1 cut(s) 1584
BsrI ACTGG 5 cut(s) 504, 608, 1439, 1484, 2062
BssECI CCNNGG 7 cut(s) 108, 525, 538, 724, 1506, 2198, 2496
BssMI GATC 4 cut(s) 1024, 2446, 2672, 2715
BssT1I CCWWGG 4 cut(s) 525, 538, 1506, 2496
Bst2UI CCWGG 3 cut(s) 414, 726, 2310
Bst4CI ACNGT 8 cut(s) 548, 569, 812, 913, 1195, 1756, 1765, 1890
Bst6I CTCTTC 3 cut(s) 162, 1557, 2223
BstAPI GCANNNNNTGC 2 cut(s) 467, 1970
BstAUI TGTACA 1 cut(s) 1584
BstC8I GCNNGC 4 cut(s) 312, 714, 1282, 2693
BstDEI CTNAG 9 cut(s) 335, 641, 681, 717, 1056, 1244, 2241, 2669, 2712
BstDSI CCRYGG 4 cut(s) 525, 538, 1506, 2198
BstEII GGTNACC 1 cut(s) 542
BstF5I GGATG 7 cut(s) 10, 58, 535, 682, 1936, 2208, 2233
BstFNI CGCG 2 cut(s) 1284, 2691
BstHHI GCGC 1 cut(s) 1286
BstKTI GATC 4 cut(s) 1027, 2449, 2675, 2718
BstMAI GTCTC 3 cut(s) 1568, 1881, 2600
BstMBI GATC 4 cut(s) 1024, 2446, 2672, 2715
BstMWI GCNNNNNNNGC 7 cut(s) 442, 458, 467, 1783, 1970, 2027, 2471
BstNI CCWGG 3 cut(s) 414, 726, 2310
BstNSI RCATGY 3 cut(s) 1100, 1133, 1748
BstPAI GACNNNNGTC 1 cut(s) 2282
BstPI GGTNACC 1 cut(s) 542
BstSCI CCNGG 3 cut(s) 412, 724, 2308
BstSFI CTRYAG 2 cut(s) 591, 1944
BstSLI GKGCMC 1 cut(s) 2167
BstUI CGCG 2 cut(s) 1284, 2691
BstV1I GCAGC 8 cut(s) 160, 445, 482, 1312, 1532, 1973, 2695, 2707
BstX2I RGATCY 1 cut(s) 2715
BstYI RGATCY 1 cut(s) 2715
Bsu15I ATCGAT 1 cut(s) 2445
Bsu36I CCTNAGG 1 cut(s) 1244
BsuI GTATCC 2 cut(s) 832, 2644
BsuRI GGCC 6 cut(s) 436, 646, 1869, 2116, 2207, 2681
BsuTUI ATCGAT 1 cut(s) 2445
BtgI CCRYGG 4 cut(s) 525, 538, 1506, 2198
BtsCI GGATG 7 cut(s) 10, 58, 535, 682, 1936, 2208, 2233
BtsI GCAGTG 1 cut(s) 1078
BtsIMutI CAGTG 2 cut(s) 511, 1078
BveI ACCTGC 1 cut(s) 2176
Cac8I GCNNGC 4 cut(s) 312, 714, 1282, 2693
CfoI GCGC 1 cut(s) 1286
Cfr13I GGNCC 2 cut(s) 2206, 2424
ClaI ATCGAT 1 cut(s) 2445
CseI GACGC 2 cut(s) 341, 942
Csp6I GTAC 7 cut(s) 808, 1295, 1585, 1891, 2235, 2255, 2306
CspCI CAANNNNNGTGG 2 cut(s) 344, 379
CviQI GTAC 7 cut(s) 808, 1295, 1585, 1891, 2235, 2255, 2306
DdeI CTNAG 9 cut(s) 335, 641, 681, 717, 1056, 1244, 2241, 2669, 2712
DpnI GATC 4 cut(s) 1026, 2448, 2674, 2717
DpnII GATC 4 cut(s) 1024, 2446, 2672, 2715
EaeI YGGCCR 2 cut(s) 1867, 2114
Eam1104I CTCTTC 3 cut(s) 162, 1557, 2223
EarI CTCTTC 3 cut(s) 162, 1557, 2223
EciI GGCGGA 1 cut(s) 1064
Eco130I CCWWGG 4 cut(s) 525, 538, 1506, 2496
Eco147I AGGCCT 1 cut(s) 646
Eco47I GGWCC 1 cut(s) 2424
Eco57I CTGAAG 4 cut(s) 186, 969, 2357, 2598
Eco81I CCTNAGG 1 cut(s) 1244
Eco88I CYCGRG 1 cut(s) 270
Eco91I GGTNACC 1 cut(s) 542
EcoO65I GGTNACC 1 cut(s) 542
EcoRI GAATTC 2 cut(s) 2299, 2358
EcoRII CCWGG 3 cut(s) 412, 724, 2308
EcoT14I CCWWGG 4 cut(s) 525, 538, 1506, 2496
EcoT22I ATGCAT 1 cut(s) 403
ErhI CCWWGG 4 cut(s) 525, 538, 1506, 2496
FauNDI CATATG 1 cut(s) 142
FbaI TGATCA 1 cut(s) 1024
FblI GTMKAC 1 cut(s) 1741
Fnu4HI GCNGC 9 cut(s) 174, 459, 471, 1326, 1521, 1962, 2696, 2709, 2744
FokI GGATG 7 cut(s) 17, 65, 542, 689, 1943, 2215, 2240
Fsp4HI GCNGC 9 cut(s) 174, 459, 471, 1326, 1521, 1962, 2696, 2709, 2744
FspBI CTAG 7 cut(s) 237, 651, 668, 938, 1568, 2037, 2646
GlaI GCGC 1 cut(s) 1285
GluI GCNGC 9 cut(s) 174, 459, 471, 1326, 1521, 1962, 2696, 2709, 2744
GsuI CTGGAG 1 cut(s) 713
HaeIII GGCC 6 cut(s) 436, 646, 1869, 2116, 2207, 2681
HapII CCGG 2 cut(s) 888, 1685
HgaI GACGC 2 cut(s) 341, 942
HhaI GCGC 1 cut(s) 1286
Hin6I GCGC 1 cut(s) 1284
HinP1I GCGC 1 cut(s) 1284
HincII GTYRAC 1 cut(s) 2367
HindII GTYRAC 1 cut(s) 2367
HpaII CCGG 2 cut(s) 888, 1685
HphI GGTGA 6 cut(s) 71, 131, 833, 1494, 1619, 1868
Hpy166II GTNNAC 6 cut(s) 895, 1585, 1587, 1742, 2165, 2367
Hpy8I GTNNAC 6 cut(s) 895, 1585, 1587, 1742, 2165, 2367
Hpy99I CGWCG 3 cut(s) 444, 1199, 2735
HpyCH4III ACNGT 8 cut(s) 548, 569, 812, 913, 1195, 1756, 1765, 1890
HpyCH4IV ACGT 2 cut(s) 774, 1849
HpyF10VI GCNNNNNNNGC 7 cut(s) 442, 458, 467, 1783, 1970, 2027, 2471
HpyF3I CTNAG 9 cut(s) 335, 641, 681, 717, 1056, 1244, 2241, 2669, 2712
HpySE526I ACGT 2 cut(s) 774, 1849
HspAI GCGC 1 cut(s) 1284
Kpn2I TCCGGA 1 cut(s) 1684
KpnI GGTACC 1 cut(s) 1298
Ksp22I TGATCA 1 cut(s) 1024
Kzo9I GATC 4 cut(s) 1024, 2446, 2672, 2715
LguI GCTCTTC 1 cut(s) 1557
LmnI GCTCC 6 cut(s) 388, 883, 1361, 1613, 2410, 2545
Lsp1109I GCAGC 8 cut(s) 160, 445, 482, 1312, 1532, 1973, 2695, 2707
LweI GCATC 5 cut(s) 46, 52, 244, 667, 2146
MaeI CTAG 7 cut(s) 237, 651, 668, 938, 1568, 2037, 2646
MaeII ACGT 2 cut(s) 774, 1849
MaeIII GTNAC 7 cut(s) 542, 622, 829, 1669, 1765, 1860, 2053
MalI GATC 4 cut(s) 1026, 2448, 2674, 2717
MboI GATC 4 cut(s) 1024, 2446, 2672, 2715
MboII GAAGA 7 cut(s) 134, 179, 767, 1574, 1658, 2002, 2210
MflI RGATCY 1 cut(s) 2715
MhlI GDGCHC 1 cut(s) 2167
MlsI TGGCCA 2 cut(s) 1869, 2116
MluNI TGGCCA 2 cut(s) 1869, 2116
MlyI GAGTC 7 cut(s) 418, 826, 953, 1883, 2095, 2630, 2735
MmeI TCCRAC 2 cut(s) 1140, 2567
Mox20I TGGCCA 2 cut(s) 1869, 2116
Mph1103I ATGCAT 1 cut(s) 403
MroI TCCGGA 1 cut(s) 1684
MscI TGGCCA 2 cut(s) 1869, 2116
MseI TTAA 8 cut(s) 347, 1103, 1677, 1977, 2145, 2331, 2382, 2420
MslI CAYNNNNRTG 5 cut(s) 396, 819, 1132, 2226, 2489
Msp20I TGGCCA 2 cut(s) 1869, 2116
MspA1I CMGCKG 2 cut(s) 473, 2746
MspI CCGG 2 cut(s) 888, 1685
MspR9I CCNGG 3 cut(s) 414, 726, 2310
Mva1269I GAATGC 3 cut(s) 472, 622, 1731
MvaI CCWGG 3 cut(s) 414, 726, 2310
MvnI CGCG 2 cut(s) 1284, 2691
MwoI GCNNNNNNNGC 7 cut(s) 442, 458, 467, 1783, 1970, 2027, 2471
NcoI CCATGG 3 cut(s) 525, 538, 1506
NdeI CATATG 1 cut(s) 142
NdeII GATC 4 cut(s) 1024, 2446, 2672, 2715
NlaIV GGNNCC 5 cut(s) 1296, 1357, 2208, 2412, 2662
NmuCI GTSAC 2 cut(s) 829, 2053
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 3 cut(s) 1100, 1133, 1748
OliI CACNNNNGTG 1 cut(s) 1132
PaqCI CACCTGC 1 cut(s) 2176
PceI AGGCCT 1 cut(s) 646
PciI ACATGT 2 cut(s) 1129, 1744
PciSI GCTCTTC 1 cut(s) 1557
PctI GAATGC 3 cut(s) 472, 622, 1731
PfeI GAWTC 5 cut(s) 596, 1220, 1313, 1378, 2372
PkrI GCNGC 9 cut(s) 175, 460, 472, 1327, 1522, 1963, 2697, 2710, 2745
PleI GAGTC 7 cut(s) 417, 826, 952, 1882, 2095, 2629, 2734
PpsI GAGTC 7 cut(s) 417, 826, 952, 1882, 2095, 2629, 2734
PscI ACATGT 2 cut(s) 1129, 1744
PshAI GACNNNNGTC 1 cut(s) 2282
PsiI TTATAA 1 cut(s) 1170
Psp6I CCWGG 3 cut(s) 412, 724, 2308
PspEI GGTNACC 1 cut(s) 542
PspGI CCWGG 3 cut(s) 412, 724, 2308
PspN4I GGNNCC 5 cut(s) 1296, 1357, 2208, 2412, 2662
PspPI GGNCC 2 cut(s) 2206, 2424
PsrI GAACNNNNNNTAC 2 cut(s) 483, 515
PstI CTGCAG 1 cut(s) 595
PsuI RGATCY 1 cut(s) 2715
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 7 cut(s) 809, 1296, 1586, 1892, 2236, 2256, 2307
RsaNI GTAC 7 cut(s) 808, 1295, 1585, 1891, 2235, 2255, 2306
RseI CAYNNNNRTG 5 cut(s) 396, 819, 1132, 2226, 2489
SapI GCTCTTC 1 cut(s) 1557
SaqAI TTAA 8 cut(s) 347, 1103, 1677, 1977, 2145, 2331, 2382, 2420
SatI GCNGC 9 cut(s) 174, 459, 471, 1326, 1521, 1962, 2696, 2709, 2744
Sau3AI GATC 4 cut(s) 1024, 2446, 2672, 2715
Sau96I GGNCC 2 cut(s) 2206, 2424
SchI GAGTC 7 cut(s) 418, 826, 953, 1883, 2095, 2630, 2735
ScrFI CCNGG 3 cut(s) 414, 726, 2310
SduI GDGCHC 1 cut(s) 2167
SfaNI GCATC 5 cut(s) 46, 52, 244, 667, 2146
SfcI CTRYAG 2 cut(s) 591, 1944
SinI GGWCC 1 cut(s) 2424
SmiMI CAYNNNNRTG 5 cut(s) 396, 819, 1132, 2226, 2489
SmlI CTYRAG 1 cut(s) 2395
SmoI CTYRAG 1 cut(s) 2395
SpeI ACTAGT 1 cut(s) 650
SseBI AGGCCT 1 cut(s) 646
SsiI CCGC 5 cut(s) 50, 1075, 2040, 2689, 2746
SspMI CTAG 7 cut(s) 237, 651, 668, 938, 1568, 2037, 2646
StuI AGGCCT 1 cut(s) 646
StyD4I CCNGG 3 cut(s) 412, 724, 2308
StyI CCWWGG 4 cut(s) 525, 538, 1506, 2496
TaaI ACNGT 8 cut(s) 548, 569, 812, 913, 1195, 1756, 1765, 1890
TaiI ACGT 2 cut(s) 777, 1852
TaqI TCGA 7 cut(s) 24, 655, 1013, 1197, 1906, 2081, 2445
TatI WGTACW 1 cut(s) 1584
TfiI GAWTC 5 cut(s) 596, 1220, 1313, 1378, 2372
Tru1I TTAA 8 cut(s) 347, 1103, 1677, 1977, 2145, 2331, 2382, 2420
Tru9I TTAA 8 cut(s) 347, 1103, 1677, 1977, 2145, 2331, 2382, 2420
TscAI CASTG 2 cut(s) 511, 1085
TseFI GTSAC 2 cut(s) 829, 2053
TseI GCWGC 9 cut(s) 173, 458, 470, 1325, 1520, 1961, 2695, 2708, 2743
Tsp45I GTSAC 2 cut(s) 829, 2053
TspDTI ATGAA 9 cut(s) 207, 576, 768, 1007, 1431, 1860, 2142, 2210, 2394
TspGWI ACGGA 5 cut(s) 113, 2215, 2250, 2352, 2719
TspRI CASTG 2 cut(s) 511, 1085
VneI GTGCAC 1 cut(s) 2163
VpaK11BI GGWCC 1 cut(s) 2424
XbaI TCTAGA 1 cut(s) 937
XceI RCATGY 3 cut(s) 1100, 1133, 1748
XcmI CCANNNNNNNNNTGG 1 cut(s) 1156
XmiI GTMKAC 1 cut(s) 1741
XspI CTAG 7 cut(s) 237, 651, 668, 938, 1568, 2037, 2646
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.