pycom06g00970

Uncharacterized protein family, UPF0114

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008652
Physical Location & Seq
Reverse (-)
950394 .. 954556
4163 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
ATGACACCGCCTCGGCCTCTGCTCTCAAAACCCATCCGAGCAGCGACACCTTCTCCGCCGTTACCATCATCCTTCAACAACGCCTCAAAACCGAGCCCAAATTGGAATAGGGCATCTGGGTACTGTCTCCATGCTCTCCGACCCATCGTCTTCATGGCCGCCAGCTCGAGGCCTGGCGTTTCTTCTGCCCCACAACACTCATCCTCCCTGCCCATCAGTTCCGATATCATTTCCGATGCCGGAGTCGGTCAGAATTCAGCAAATTCACCAAATAAAGTAGAAGCCGTCGAGGAATACTTAGACAAGGTGGTATACAGATTTCGGTTCTTGCCGTTTATGGGAGTTTTGGGGTCTTTGATTGGGTCATTTCTTTGTTTCATCAAGGGCTGCACTTATGTGGTACAGTCTTTCATGGAGTACTCTGTAAATCGCAGTAAAGTAATCTGGTCGCTGGTTGAGGCTATTGATGTTTATCTTTTGGGAACAGTGATGTTGGTGTTTGGAATGGGTCTTTATGAGCTCTTTGTCAGTAATCTCGGTCCTTCGGAATGTTCGGCAGATGATAAAGCTTCTCACAAATCAAATCTTTTTGGCATGTTCACTCTAAAGGAGCGACCGAAATGGTTGGATATCACAAGCGTGAATGAGCTGAAAACGAAGCTTGGCCATGTAATAGTGATGCTACTTCTAATTGGGTTGTTCGAGAAGAGTAAGAAAGCTGTTATACAATCTCCTCTGGACTTGCTTTGTTTTTCCGCGTCTGTCCTCCTTGCTTCTGTTTGCCTCTTTTTATTGTCTAAGCTCAGCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0114

Protein Analysis

271

Amino Acids

29.45

Weight (kDa)

8.99

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0114 PF03350 105 - 236 9.8e-36 Uncharacterized protein family, UPF0114
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 312
AccII CGCG 1 cut(s) 758
AciI CCGC 4 cut(s) 8, 56, 159, 756
AcoI YGGCCR 2 cut(s) 156, 664
AcsI RAATTY 2 cut(s) 253, 262
AfaI GTAC 3 cut(s) 122, 402, 419
AfiI CCNNNNNNNGG 2 cut(s) 168, 338
AgsI TTSAA 1 cut(s) 76
AhdI GACNNNNNGTC 1 cut(s) 146
AjnI CCWGG 1 cut(s) 172
AleI CACNNNNGTG 2 cut(s) 395, 638
AluBI AGCT 7 cut(s) 165, 520, 569, 649, 661, 719, 802
AluI AGCT 7 cut(s) 165, 520, 569, 649, 661, 719, 802
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 166
AoxI GGCC 4 cut(s) 14, 156, 170, 664
ApeKI GCWGC 2 cut(s) 41, 387
ApoI RAATTY 2 cut(s) 253, 262
AspS9I GGNCC 1 cut(s) 539
AsuHPI GGTGA 1 cut(s) 258
AvaI CYCGRG 1 cut(s) 166
AvaII GGWCC 1 cut(s) 539
BalI TGGCCA 1 cut(s) 666
BanII GRGCYC 2 cut(s) 98, 522
BbsI GAAGAC 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 522
BbvI GCAGC 2 cut(s) 53, 374
BccI CCATC 4 cut(s) 41, 73, 152, 221
BceAI ACGGC 3 cut(s) 43, 269, 316
BciT130I CCWGG 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 1 cut(s) 811
BisI GCNGC 3 cut(s) 42, 159, 388
BlpI GCTNAGC 1 cut(s) 803
BlsI GCNGC 3 cut(s) 43, 160, 389
BmcAI AGTACT 1 cut(s) 419
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 539
BmeRI GACNNNNNGTC 1 cut(s) 146
BmeT110I CYCGRG 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 539
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 3 cut(s) 122, 226, 669
BpiI GAAGAC 1 cut(s) 142
Bpu1102I GCTNAGC 1 cut(s) 803
BsaBI GATNNNNATC 1 cut(s) 471
BsaJI CCNNGG 1 cut(s) 11
BsaXI ACNNNNNCTCC 4 cut(s) 37, 67, 188, 218
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 338
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 3 cut(s) 33, 68, 200
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 2 cut(s) 168, 338
BseRI GAGGAG 1 cut(s) 723
BseXI GCAGC 2 cut(s) 53, 374
BsgI GTGCAG 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 758
Bsh1285I CGRYCG 1 cut(s) 617
BshFI GGCC 4 cut(s) 16, 158, 172, 666
BsiEI CGRYCG 1 cut(s) 617
BsiHKAI GWGCWC 1 cut(s) 522
BsiHKCI CYCGRG 1 cut(s) 166
BsiSI CCGG 1 cut(s) 240
BslI CCNNNNNNNGG 2 cut(s) 168, 338
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 4 cut(s) 16, 158, 172, 666
BsoBI CYCGRG 1 cut(s) 166
Bsp1286I GDGCHC 2 cut(s) 98, 522
Bsp1720I GCTNAGC 1 cut(s) 803
BspACI CCGC 4 cut(s) 8, 56, 159, 756
BspANI GGCC 4 cut(s) 16, 158, 172, 666
BspFNI CGCG 1 cut(s) 758
BssECI CCNNGG 1 cut(s) 11
BssNAI GTATAC 1 cut(s) 313
Bst1107I GTATAC 1 cut(s) 313
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 3 cut(s) 125, 405, 487
Bst6I CTCTTC 1 cut(s) 701
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 3 cut(s) 298, 798, 803
BstF5I GGATG 3 cut(s) 33, 68, 200
BstFNI CGCG 1 cut(s) 758
BstMAI GTCTC 1 cut(s) 131
BstMCI CGRYCG 1 cut(s) 617
BstNI CCWGG 1 cut(s) 174
BstNSI RCATGY 1 cut(s) 598
BstSCI CCNGG 1 cut(s) 172
BstUI CGCG 1 cut(s) 758
BstV1I GCAGC 2 cut(s) 53, 374
BstV2I GAAGAC 1 cut(s) 142
BstZ17I GTATAC 1 cut(s) 313
BsuRI GGCC 4 cut(s) 16, 158, 172, 666
BtsCI GGATG 3 cut(s) 33, 68, 200
BtsIMutI CAGTG 1 cut(s) 492
Cac8I GCNNGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 539
CseI GACGC 1 cut(s) 747
Csp6I GTAC 3 cut(s) 121, 401, 418
CviAII CATG 5 cut(s) 131, 154, 412, 595, 668
CviQI GTAC 3 cut(s) 121, 401, 418
DdeI CTNAG 3 cut(s) 298, 798, 803
DriI GACNNNNNGTC 1 cut(s) 146
EaeI YGGCCR 2 cut(s) 156, 664
Eam1104I CTCTTC 1 cut(s) 701
Eam1105I GACNNNNNGTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 701
EciI GGCGGA 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 520
Eco147I AGGCCT 1 cut(s) 172
Eco24I GRGCYC 2 cut(s) 98, 522
Eco32I GATATC 2 cut(s) 226, 631
Eco47I GGWCC 1 cut(s) 539
Eco53kI GAGCTC 1 cut(s) 520
Eco88I CYCGRG 1 cut(s) 166
EcoICRI GAGCTC 1 cut(s) 520
EcoRI GAATTC 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 172
EcoRV GATATC 2 cut(s) 226, 631
EcoT38I GRGCYC 2 cut(s) 98, 522
FaeI CATG 5 cut(s) 134, 157, 415, 598, 671
FatI CATG 5 cut(s) 130, 153, 411, 594, 667
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 3 cut(s) 42, 159, 388
FokI GGATG 3 cut(s) 20, 55, 187
FriOI GRGCYC 2 cut(s) 98, 522
Fsp4HI GCNGC 3 cut(s) 42, 159, 388
FspBI CTAG 1 cut(s) 811
GluI GCNGC 3 cut(s) 42, 159, 388
HaeIII GGCC 4 cut(s) 16, 158, 172, 666
HapII CCGG 1 cut(s) 240
HgaI GACGC 1 cut(s) 747
Hin1II CATG 5 cut(s) 134, 157, 415, 598, 671
HindIII AAGCTT 2 cut(s) 567, 659
HinfI GANTC 1 cut(s) 243
HpaII CCGG 1 cut(s) 240
HphI GGTGA 1 cut(s) 258
Hpy166II GTNNAC 2 cut(s) 313, 600
Hpy188I TCNGA 6 cut(s) 38, 140, 223, 235, 252, 547
Hpy188III TCNNGA 2 cut(s) 703, 737
Hpy8I GTNNAC 2 cut(s) 313, 600
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 3 cut(s) 60, 82, 552
HpyCH4III ACNGT 3 cut(s) 125, 405, 487
HpyCH4V TGCA 1 cut(s) 390
HpyF3I CTNAG 3 cut(s) 298, 798, 803
Hsp92II CATG 5 cut(s) 134, 157, 415, 598, 671
LmnI GCTCC 1 cut(s) 610
LpnPI CCDG 9 cut(s) 102, 159, 175, 186, 221, 253, 430, 437, 722
Lsp1109I GCAGC 2 cut(s) 53, 374
LweI GCATC 3 cut(s) 122, 226, 669
MaeI CTAG 1 cut(s) 811
MaeIII GTNAC 1 cut(s) 60
MboII GAAGA 3 cut(s) 142, 174, 718
MhlI GDGCHC 2 cut(s) 98, 522
MlsI TGGCCA 1 cut(s) 666
MluCI AATT 4 cut(s) 100, 253, 262, 690
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 1 cut(s) 252
MmeI TCCRAC 2 cut(s) 163, 606
Mox20I TGGCCA 1 cut(s) 666
MscI TGGCCA 1 cut(s) 666
MslI CAYNNNNRTG 2 cut(s) 395, 638
Msp20I TGGCCA 1 cut(s) 666
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 1 cut(s) 174
MvaI CCWGG 1 cut(s) 174
MvnI CGCG 1 cut(s) 758
NlaIII CATG 5 cut(s) 134, 157, 415, 598, 671
NspI RCATGY 1 cut(s) 598
OliI CACNNNNGTG 2 cut(s) 395, 638
PaeR7I CTCGAG 1 cut(s) 166
PceI AGGCCT 1 cut(s) 172
PkrI GCNGC 3 cut(s) 43, 160, 389
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 172
PspGI CCWGG 1 cut(s) 172
PspPI GGNCC 1 cut(s) 539
PspXI VCTCGAGB 1 cut(s) 166
RsaI GTAC 3 cut(s) 122, 402, 419
RsaNI GTAC 3 cut(s) 121, 401, 418
RseI CAYNNNNRTG 2 cut(s) 395, 638
SacI GAGCTC 1 cut(s) 522
SatI GCNGC 3 cut(s) 42, 159, 388
Sau96I GGNCC 1 cut(s) 539
ScaI AGTACT 1 cut(s) 419
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 2 cut(s) 98, 522
SetI ASST 9 cut(s) 52, 167, 309, 522, 571, 651, 663, 721, 804
SfaNI GCATC 3 cut(s) 122, 226, 669
Sfr274I CTCGAG 1 cut(s) 166
SinI GGWCC 1 cut(s) 539
SlaI CTCGAG 1 cut(s) 166
SmiMI CAYNNNNRTG 2 cut(s) 395, 638
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 4 cut(s) 100, 253, 262, 690
SseBI AGGCCT 1 cut(s) 172
SsiI CCGC 4 cut(s) 8, 56, 159, 756
SspMI CTAG 1 cut(s) 811
SstI GAGCTC 1 cut(s) 522
StuI AGGCCT 1 cut(s) 172
StyD4I CCNGG 1 cut(s) 172
TaaI ACNGT 3 cut(s) 125, 405, 487
TaqI TCGA 3 cut(s) 167, 288, 702
TaqII GACCGA 3 cut(s) 236, 527, 631
TasI AATT 4 cut(s) 100, 253, 262, 690
TatI WGTACW 1 cut(s) 417
TauI GCSGC 1 cut(s) 161
TscAI CASTG 1 cut(s) 492
TseI GCWGC 2 cut(s) 41, 387
TspDTI ATGAA 3 cut(s) 142, 367, 400
TspRI CASTG 1 cut(s) 492
VpaK11BI GGWCC 1 cut(s) 539
XapI RAATTY 2 cut(s) 253, 262
XceI RCATGY 1 cut(s) 598
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XhoI CTCGAG 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 1 cut(s) 811
ZrmI AGTACT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.