pycom06g01160

Protein of unknown function (DUF3464)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0015437
Physical Location & Seq
Reverse (-)
1225104 .. 1226225
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGGCTGCAATTGCAGCAGGGCTCGCTTCATGGTCATCATCCAAGCCCTCCCTGATTCCTTCCCAGCACCAAATATCATCACCATCATCATCACCTAATAAGTTTGACGCTAAGCCTTGGAGCCTCTCCATGACCAGCAGTTTAAATGAGGATATAAAAATCCAGTACCCAATCTCCGCAACAAAACAATGCCCAAATACTCAGAAACAATTGAATGCCACCTTGAGAAGCCCAAGAGGCTTCGGACCCTCCCCAAGGAAAAAGAAAAAGATTACCGAGAAGCCGAAGAAGACCCGAAACAATGATAGCGACGACGACGACGACGATGAAGATGAAGATGAGGAGGAAGGAGAGGAAGGAGTGATACCAGAGGTGGTGACCAACAGGATGATTGGAAGGATGGGCTTCACAGTTGGGCTTCCATTATTTTTGGGCCTCTTGTTTTTCCCTTTCTTTTACTATTTGAAAGTTGGTCTGAAAATTGATGTTCCCACCTGGGTGCCCTTCATTGTGTCATTTGTCTTCTTTGGGACAGCCCTATTGGGGGTCAGCTATGGGATTGTGTCCTCCAGCTGGGATCCTATGAGGGAAGGCTCCCTCTTGGGTTGGACTGAAGCTCAAAAAAATTGGCCTGTATTTTGGCGGTCCCTTCGTGGTGGTGGATCGAAAAAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3464

Protein Analysis

226

Amino Acids

25.0

Weight (kDa)

7.75

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3464 PF11947 81 - 216 1.2e-50 Photosynthesis affected mutant 68
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19100 AT4G19100
fragaria_vesca FvH4_5g23870
malus_domestica MD06G1014500.v1.1
prunus_persica Prupe.5G021700_v2.0.a1
pyrus_communis pycom06g01160
rosa_chinensis RchiOBHm_Chr7g0213261
rosa_laevigata RLG00000002817
rosa_multiflora Rmu_ssc0000084.1_g000025
rosa_roxburghii Rroxscaffold_3G00246130
rosa_rugosa Rorug07G0140900
rosa_samantha Rh7AG272400 Rh7BG268100 Rh7CG291800 Rh7DG280300
rosa_wichuraiana Rw7G023640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 499
AciI CCGC 2 cut(s) 177, 643
AclWI GGATC 3 cut(s) 572, 585, 670
AcuI CTGAAG 1 cut(s) 633
AfaI GTAC 1 cut(s) 167
AfiI CCNNNNNNNGG 4 cut(s) 255, 543, 544, 573
AgsI TTSAA 2 cut(s) 214, 466
AjnI CCWGG 1 cut(s) 494
AleI CACNNNNGTG 1 cut(s) 497
AluBI AGCT 3 cut(s) 552, 573, 617
AluI AGCT 3 cut(s) 552, 573, 617
AlwI GGATC 3 cut(s) 572, 585, 670
AoxI GGCC 2 cut(s) 433, 629
ApeKI GCWGC 2 cut(s) 5, 14
AspS9I GGNCC 3 cut(s) 245, 433, 645
AsuHPI GGTGA 3 cut(s) 72, 84, 388
AvaII GGWCC 2 cut(s) 245, 645
BaeGI GKGCMC 1 cut(s) 504
BamHI GGATCC 1 cut(s) 577
BanI GGYRCC 1 cut(s) 499
BanII GRGCYC 1 cut(s) 24
BarI GAAGNNNNNNTAC 2 cut(s) 348, 380
BbsI GAAGAC 2 cut(s) 296, 514
BbvI GCAGC 1 cut(s) 26
BccI CCATC 2 cut(s) 91, 394
BciT130I CCWGG 1 cut(s) 496
BglI GCCNNNNNGGC 1 cut(s) 237
BisI GCNGC 2 cut(s) 6, 15
BlpI GCTNAGC 1 cut(s) 111
BlsI GCNGC 2 cut(s) 7, 16
Bme1390I CCNGG 1 cut(s) 496
Bme18I GGWCC 2 cut(s) 245, 645
BmgT120I GGNCC 3 cut(s) 245, 433, 645
BmiI GGNNCC 6 cut(s) 122, 247, 501, 579, 595, 647
BmrFI CCNGG 1 cut(s) 496
BpiI GAAGAC 2 cut(s) 296, 514
BpmI CTGGAG 1 cut(s) 553
Bpu1102I GCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 244
BsaJI CCNNGG 3 cut(s) 116, 254, 495
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 4 cut(s) 255, 543, 544, 573
Bse1I ACTGG 1 cut(s) 163
BseBI CCWGG 1 cut(s) 496
BseDI CCNNGG 3 cut(s) 116, 254, 495
BseGI GGATG 3 cut(s) 38, 393, 405
BseLI CCNNNNNNNGG 4 cut(s) 255, 543, 544, 573
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 163
BseRI GAGGAG 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 1 cut(s) 26
BseYI CCCAGC 2 cut(s) 63, 573
BshFI GGCC 2 cut(s) 435, 631
BshNI GGYRCC 1 cut(s) 499
BslFI GGGAC 2 cut(s) 544, 631
BslI CCNNNNNNNGG 4 cut(s) 255, 543, 544, 573
BsmFI GGGAC 2 cut(s) 544, 631
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 2 cut(s) 435, 631
Bsp1286I GDGCHC 2 cut(s) 24, 504
Bsp143I GATC 2 cut(s) 577, 662
Bsp1720I GCTNAGC 1 cut(s) 111
BspACI CCGC 2 cut(s) 177, 643
BspANI GGCC 2 cut(s) 435, 631
BspCNI CTCAG 1 cut(s) 214
BspLI GGNNCC 6 cut(s) 122, 247, 501, 579, 595, 647
BspPI GGATC 3 cut(s) 572, 585, 670
BspT107I GGYRCC 1 cut(s) 499
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 3 cut(s) 116, 254, 495
BssMI GATC 2 cut(s) 577, 662
BssT1I CCWWGG 2 cut(s) 116, 254
Bst2UI CCWGG 1 cut(s) 496
Bst4CI ACNGT 1 cut(s) 412
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 2 cut(s) 111, 201
BstEII GGTNACC 1 cut(s) 376
BstENI CCTNNNNNAGG 1 cut(s) 253
BstF5I GGATG 3 cut(s) 38, 393, 405
BstKTI GATC 2 cut(s) 580, 665
BstMBI GATC 2 cut(s) 577, 662
BstMWI GCNNNNNNNGC 4 cut(s) 11, 14, 23, 237
BstNI CCWGG 1 cut(s) 496
BstPI GGTNACC 1 cut(s) 376
BstSCI CCNGG 1 cut(s) 494
BstSLI GKGCMC 1 cut(s) 504
BstV1I GCAGC 1 cut(s) 26
BstV2I GAAGAC 2 cut(s) 296, 514
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BsuRI GGCC 2 cut(s) 435, 631
BtsCI GGATG 3 cut(s) 38, 393, 405
Cac8I GCNNGC 1 cut(s) 24
Cfr13I GGNCC 3 cut(s) 245, 433, 645
CseI GACGC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 166
CviAII CATG 2 cut(s) 30, 130
CviQI GTAC 1 cut(s) 166
DdeI CTNAG 2 cut(s) 111, 201
DpnI GATC 2 cut(s) 579, 664
DpnII GATC 2 cut(s) 577, 662
DraI TTTAAA 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 116, 254
Eco24I GRGCYC 1 cut(s) 24
Eco47I GGWCC 2 cut(s) 245, 645
Eco57I CTGAAG 1 cut(s) 633
Eco91I GGTNACC 1 cut(s) 376
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoO65I GGTNACC 1 cut(s) 376
EcoRII CCWGG 1 cut(s) 494
EcoT14I CCWWGG 2 cut(s) 116, 254
EcoT38I GRGCYC 1 cut(s) 24
ErhI CCWWGG 2 cut(s) 116, 254
FaeI CATG 2 cut(s) 33, 133
FaiI YATR 5 cut(s) 31, 131, 155, 555, 584
FaqI GGGAC 2 cut(s) 544, 631
FatI CATG 2 cut(s) 29, 129
Fnu4HI GCNGC 2 cut(s) 6, 15
FokI GGATG 3 cut(s) 25, 400, 412
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 2 cut(s) 6, 15
GluI GCNGC 2 cut(s) 6, 15
GsaI CCCAGC 2 cut(s) 67, 577
GsuI CTGGAG 1 cut(s) 553
HaeIII GGCC 2 cut(s) 435, 631
HgaI GACGC 1 cut(s) 116
Hin1II CATG 2 cut(s) 33, 133
HinfI GANTC 1 cut(s) 55
HphI GGTGA 3 cut(s) 72, 84, 388
Hpy188I TCNGA 3 cut(s) 204, 245, 477
Hpy99I CGWCG 5 cut(s) 314, 317, 320, 323, 326
HpyAV CCTTC 7 cut(s) 69, 341, 350, 390, 514, 584, 659
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4V TGCA 2 cut(s) 8, 14
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 14, 23, 237
HpyF3I CTNAG 2 cut(s) 111, 201
Hsp92II CATG 2 cut(s) 33, 133
Kzo9I GATC 2 cut(s) 577, 662
LmnI GCTCC 2 cut(s) 120, 599
Lsp1109I GCAGC 1 cut(s) 26
MaeIII GTNAC 1 cut(s) 376
MalI GATC 2 cut(s) 579, 664
MboI GATC 2 cut(s) 577, 662
MboII GAAGA 5 cut(s) 298, 301, 341, 347, 514
MfeI CAATTG 2 cut(s) 9, 209
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 2 cut(s) 24, 504
MluCI AATT 4 cut(s) 9, 209, 480, 625
MmeI TCCRAC 1 cut(s) 587
MseI TTAA 1 cut(s) 143
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 1 cut(s) 573
MspR9I CCNGG 1 cut(s) 496
MunI CAATTG 2 cut(s) 9, 209
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 1 cut(s) 496
MwoI GCNNNNNNNGC 4 cut(s) 11, 14, 23, 237
NdeII GATC 2 cut(s) 577, 662
NlaIII CATG 2 cut(s) 33, 133
NlaIV GGNNCC 6 cut(s) 122, 247, 501, 579, 595, 647
NmuCI GTSAC 1 cut(s) 376
OliI CACNNNNGTG 1 cut(s) 497
PcsI WCGNNNNNNNCGW 2 cut(s) 318, 321
PctI GAATGC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 55
PkrI GCNGC 2 cut(s) 7, 16
Psp6I CCWGG 1 cut(s) 494
PspEI GGTNACC 1 cut(s) 376
PspFI CCCAGC 2 cut(s) 63, 573
PspGI CCWGG 1 cut(s) 494
PspN4I GGNNCC 6 cut(s) 122, 247, 501, 579, 595, 647
PspPI GGNCC 3 cut(s) 245, 433, 645
PsuI RGATCY 1 cut(s) 577
PvuII CAGCTG 1 cut(s) 573
RsaI GTAC 1 cut(s) 167
RsaNI GTAC 1 cut(s) 166
RseI CAYNNNNRTG 1 cut(s) 497
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 2 cut(s) 6, 15
Sau3AI GATC 2 cut(s) 577, 662
Sau96I GGNCC 3 cut(s) 245, 433, 645
ScrFI CCNGG 1 cut(s) 496
SduI GDGCHC 2 cut(s) 24, 504
SetI ASST 7 cut(s) 97, 224, 375, 497, 554, 575, 619
SinI GGWCC 2 cut(s) 245, 645
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 4 cut(s) 9, 209, 480, 625
SsiI CCGC 2 cut(s) 177, 643
StyD4I CCNGG 1 cut(s) 494
StyI CCWWGG 2 cut(s) 116, 254
TaaI ACNGT 1 cut(s) 412
TaqI TCGA 1 cut(s) 665
TasI AATT 4 cut(s) 9, 209, 480, 625
TfiI GAWTC 1 cut(s) 55
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 2 cut(s) 5, 14
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 4 cut(s) 18, 342, 348, 496
VpaK11BI GGWCC 2 cut(s) 245, 645
XagI CCTNNNNNAGG 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.