pycom06g01750

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004152
Physical Location & Seq
Reverse (-)
2187519 .. 2188732
1214 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
ATGGCTGATTTAGAATTGGAGCTTGATTTATCCCCAAAACCTATTAAGATCATGGAGCAAAGGTTATCGTTTATCGAGCAGAGGAGTGGTTTTCTTCACAGTCTCATTAACCAGTATGCGAGGGCGAACAAGGAGCTACTGAAACTCAGCACTTCCTTGGACCTTATAAGTCGTCTAAAAGCCACAACCAAGGAGATTAATGGTTTGAGGACTCTGATCGATCAATGTTCTCAAGAAAAAGACATGCTTGATATGGTAACTACCAAATTGATCGATCAAGCCATCCCCAAGGAAAGAAAGCTACACAATTTATTGCTCAAGTCCCTGGTTCCTAAGGATGATGCTGATGAGAGGGATTGCATACTAGAGGTCGGAGCAGGAACTGGTGGGGATGAGGCTTCTTTGTTTGCAATGGACATATTTAAAATATGCATGACTGAATTTATTCCCTTTCTTAAGGTTTCTTTTGTTAGCAAGGACTCCTTTATGTTGGATGGACTTGACAATGCTAGTGCTAGGTTGAACGAGATTATTACTTCTGGTTTAAAGACGGGTAATAATAGTGCAACACCTCCTTGGATGGCTGATGAAGGTGGTCTGCCTTCAGATACTTCTGAACTTCTACCTAAATTGGTAATTGCATTCGGTTTCCTCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

220

Amino Acids

24.28

Weight (kDa)

4.9

Isoelectric Point (pI)

26.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 36 - 143 1.6e-17 PCRF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 167
AcsI RAATTY 1 cut(s) 440
AcuI CTGAAG 1 cut(s) 588
AflII CTTAAG 1 cut(s) 455
AgsI TTSAA 1 cut(s) 523
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 3 cut(s) 22, 136, 301
AluI AGCT 3 cut(s) 22, 136, 301
Alw26I GTCTC 1 cut(s) 107
AlwNI CAGNNNCTG 1 cut(s) 383
ApoI RAATTY 1 cut(s) 440
AseI ATTAAT 1 cut(s) 198
Asp700I GAANNNNTTC 1 cut(s) 444
AspS9I GGNCC 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 646
AvaII GGWCC 1 cut(s) 160
AxyI CCTNAGG 1 cut(s) 333
BccI CCATC 3 cut(s) 290, 488, 574
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 3 cut(s) 365, 510, 516
BfrI CTTAAG 1 cut(s) 455
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 1 cut(s) 331
BpuEI CTTGAG 2 cut(s) 216, 302
Bsa29I ATCGAT 2 cut(s) 219, 273
BsaJI CCNNGG 5 cut(s) 156, 189, 288, 324, 575
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bse1I ACTGG 2 cut(s) 112, 388
Bse21I CCTNAGG 1 cut(s) 333
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 1 cut(s) 326
BseCI ATCGAT 2 cut(s) 219, 273
BseDI CCNNGG 5 cut(s) 156, 189, 288, 324, 575
BseGI GGATG 5 cut(s) 282, 343, 397, 499, 585
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 1 cut(s) 160
BseNI ACTGG 2 cut(s) 112, 388
BseRI GAGGAG 1 cut(s) 97
BshVI ATCGAT 2 cut(s) 219, 273
BslFI GGGAC 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 307
BsmI GAATGC 1 cut(s) 641
Bsp143I GATC 5 cut(s) 48, 216, 220, 270, 274
BspCNI CTCAG 1 cut(s) 159
BspDI ATCGAT 2 cut(s) 219, 273
BspLI GGNNCC 1 cut(s) 330
BspTI CTTAAG 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 417
BsrI ACTGG 2 cut(s) 112, 388
BssECI CCNNGG 5 cut(s) 156, 189, 288, 324, 575
BssMI GATC 5 cut(s) 48, 216, 220, 270, 274
BssT1I CCWWGG 4 cut(s) 156, 189, 288, 575
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 455
BstDEI CTNAG 2 cut(s) 146, 333
BstF5I GGATG 5 cut(s) 282, 343, 397, 499, 585
BstKTI GATC 5 cut(s) 51, 219, 223, 273, 277
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 5 cut(s) 48, 216, 220, 270, 274
BstNI CCWGG 1 cut(s) 326
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 1 cut(s) 324
Bsu15I ATCGAT 2 cut(s) 219, 273
Bsu36I CCTNAGG 1 cut(s) 333
BsuTUI ATCGAT 2 cut(s) 219, 273
BtsCI GGATG 5 cut(s) 282, 343, 397, 499, 585
CaiI CAGNNNCTG 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 160
ClaI ATCGAT 2 cut(s) 219, 273
CviAII CATG 3 cut(s) 52, 244, 433
CviJI RGCY 8 cut(s) 5, 22, 136, 182, 281, 301, 398, 584
CviKI_1 RGCY 8 cut(s) 5, 22, 136, 182, 281, 301, 398, 584
DdeI CTNAG 2 cut(s) 146, 333
DpnI GATC 5 cut(s) 50, 218, 222, 272, 276
DpnII GATC 5 cut(s) 48, 216, 220, 270, 274
DraI TTTAAA 2 cut(s) 424, 546
Eco130I CCWWGG 4 cut(s) 156, 189, 288, 575
Eco47I GGWCC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 588
Eco81I CCTNAGG 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 324
EcoT14I CCWWGG 4 cut(s) 156, 189, 288, 575
EcoT22I ATGCAT 1 cut(s) 434
ErhI CCWWGG 4 cut(s) 156, 189, 288, 575
FaeI CATG 3 cut(s) 55, 247, 436
FalI AAGNNNNNCTT 4 cut(s) 231, 263, 467, 499
FaqI GGGAC 1 cut(s) 307
FatI CATG 3 cut(s) 51, 243, 432
FokI GGATG 5 cut(s) 269, 350, 404, 506, 592
FspBI CTAG 3 cut(s) 365, 510, 516
Hin1II CATG 3 cut(s) 55, 247, 436
HinfI GANTC 2 cut(s) 211, 479
HphI GGTGA 1 cut(s) 646
Hpy188I TCNGA 4 cut(s) 216, 374, 607, 616
Hpy188III TCNNGA 1 cut(s) 233
HpyAV CCTTC 2 cut(s) 584, 612
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4V TGCA 5 cut(s) 360, 410, 432, 566, 641
HpyF3I CTNAG 2 cut(s) 146, 333
Hsp92II CATG 3 cut(s) 55, 247, 436
Kzo9I GATC 5 cut(s) 48, 216, 220, 270, 274
LmnI GCTCC 4 cut(s) 19, 55, 133, 374
LpnPI CCDG 6 cut(s) 125, 311, 338, 363, 369, 525
LweI GCATC 1 cut(s) 331
MaeI CTAG 3 cut(s) 365, 510, 516
MaeIII GTNAC 1 cut(s) 256
MalI GATC 5 cut(s) 50, 218, 222, 272, 276
MboI GATC 5 cut(s) 48, 216, 220, 270, 274
MboII GAAGA 1 cut(s) 86
MluCI AATT 6 cut(s) 14, 266, 307, 440, 629, 636
MlyI GAGTC 2 cut(s) 205, 473
MmeI TCCRAC 2 cut(s) 352, 471
MnlI CCTC 7 cut(s) 75, 114, 201, 345, 361, 388, 582
Mph1103I ATGCAT 1 cut(s) 434
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 6 cut(s) 45, 108, 198, 423, 456, 545
MspCI CTTAAG 1 cut(s) 455
MspR9I CCNGG 1 cut(s) 326
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 1 cut(s) 326
NdeII GATC 5 cut(s) 48, 216, 220, 270, 274
NlaIII CATG 3 cut(s) 55, 247, 436
NlaIV GGNNCC 1 cut(s) 330
NsiI ATGCAT 1 cut(s) 434
NspI RCATGY 1 cut(s) 247
PctI GAATGC 1 cut(s) 641
PdmI GAANNNNTTC 1 cut(s) 444
PleI GAGTC 2 cut(s) 205, 473
PpsI GAGTC 2 cut(s) 205, 473
PshBI ATTAAT 1 cut(s) 198
PsiI TTATAA 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 383
SaqAI TTAA 6 cut(s) 45, 108, 198, 423, 456, 545
Sau3AI GATC 5 cut(s) 48, 216, 220, 270, 274
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 2 cut(s) 205, 473
ScrFI CCNGG 1 cut(s) 326
SfaNI GCATC 1 cut(s) 331
SinI GGWCC 1 cut(s) 160
SmlI CTYRAG 3 cut(s) 231, 317, 455
SmoI CTYRAG 3 cut(s) 231, 317, 455
Sse9I AATT 6 cut(s) 14, 266, 307, 440, 629, 636
SspMI CTAG 3 cut(s) 365, 510, 516
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 4 cut(s) 156, 189, 288, 575
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 3 cut(s) 75, 219, 273
TasI AATT 6 cut(s) 14, 266, 307, 440, 629, 636
Tru1I TTAA 6 cut(s) 45, 108, 198, 423, 456, 545
Tru9I TTAA 6 cut(s) 45, 108, 198, 423, 456, 545
TspDTI ATGAA 1 cut(s) 603
Vha464I CTTAAG 1 cut(s) 455
VpaK11BI GGWCC 1 cut(s) 160
VspI ATTAAT 1 cut(s) 198
XapI RAATTY 1 cut(s) 440
XceI RCATGY 1 cut(s) 247
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 3 cut(s) 365, 510, 516
Zsp2I ATGCAT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.