Rmu_ssc0000440.1_g000010

Belongs to the histidine acid phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000440.1
OG0004152
Physical Location & Seq
Reverse (-)
39431 .. 55303
15873 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000440.1_g000010.1.cds

Sequence Viewer

Length: 4221 bp
atggagaagaagattaagattggggtttgtgtgatggaaaagaaggtgttctccgcgccgatggggcagattctcgagcgccttcaggaatttggcgaattcgagattacacatttcggtgacaaggtcattctggaagatccggttgagagctggccgatttgtgattgcttgattgccttccattcctctggctatcctctggaaaaggctgaagcatatgctaatttaaggaaaccttttctcgtaaacgagttggagccgcaacaccttcttcatgaccgaaggaaggtatatgagcgtcttgaaatgtatggaattcctgttccgagatatgcccttgtaaatagagaagcaccatatcaagagctcgactattttgttgaggaagaagattttgttgaggttcatggtcaacgtttctggaagccatttgtggagaagcctgttgaggggaatgatcacagtataatgatatattaccctagctcggcaggtggaggaatgaaggagttgtttaggaaggttggtaaccgatctagtgagttccatccagaggtgagaagagtgagacgagaaggctcttatatatatgaggaattcatgccaactggaggcacagatgtcaaggtgtacacggtgggccctgaatatgcgcatgctgaggcaaggaaatctcctgttgtagatggtgttgtcatgagaaatcctgatggcaaggaagtgagatatcctgttttactgacacctacggagaagcaaatggcaagggaggtctgcattgcatttaggcaagcggtttgtgggtttgatcttttacgttgcgagggacgctcctatgtctgtgatgtgaatggatggagtttcgtgaagaattcctacaagtattatgatgatgctgcttgtgttctcaggaagatatttatagatgctaaagctcctcatctttcttctgtaattccaccaactttaccatggaaaatcaatgaaccaagccagccttctgagggtctaactcgccaaggaagtggaatcattggcacatttgggcaggctgaggaactacgttgcgtaattgccattattcgtcatggtgatagaactcccaaacagaaagtaaagctgaaggttaccgaggaaaagcttctaaacttgatgctaaagtacaatgggggccgacctagatctgagacaaaactgaaaagtgctatccaattgcaagatttgttggatgccacacggatgcttgtacctcgttctagaccaggtcgagaaagtgacagtgaggcagaagacattgagcatgctgaaaagcttcgtcaagttaaagcagtgctcgaggagcgtcttgaaatgtatggaattcctgttccgagatatgcccttgtaaatagagaagcaccatatcaagagctcgactattttgttgaggaagaagattttgttgaggttcatggtcaacgtttctggaagccatttgtggagaagcctgttgaggggaatgatcacagtataatgatatattaccctagctcggcaggtggaggaatgaaggagttgtttaggaaggttggtaaccgatctagtgagttccatccagaggtgagaagagtgagacgagaaggctcttatatatatgaggaattcatgccaactggaggcacagatgtcaaggtgtacacggtgggccctgaatatgcgcatgctgaggcaaggaaatctcctgttgtagatggtgttgtcatgagaaatcctgatggcaaggaagtgagatatcctgttttactgacacctacggagaagcaaatggcaagggaggtctgcattgcatttaggcaagcggtttgtgggtttgatcttttacgttgcgagggacgctcctatgtctgtgatgtgaatggatggagtttcgtgaagaattcctacaagtattatgatgatgctgcttgtgttctcaggaagatatttatagatgctaaagctcctcatctttcttctgtaattccaccaactttaccatggaaaatcaatgaaccaagccagccttctgagggtctaactcgccaaggaagtggaatcattggcacatttgggcaggctgaggaactacgttgcgtaattgccattattcgtcatggtgatagaactcccaaacagaaagtaaagctgaaggttaccgaggaaaagcttctaaacttgatgctaaagtacaatgggggccgacctagatctgagacaaaactgaaaagtgctatccaattgcaagatttgttagatgccacacggattcttgtacctcgttctagaccaggtcgagaaagtgacagtgaggcagaagacattgagcatgctgaaaagcttcgtcaagttaaagcagtgctcgaggaggggggacacttctcaggcatttataggaaagttcagctaaagccgctgaagtgggataaagttgctaaaagtaatggtgaaggtgaggaagaacgccctgttgaagctttgatggttcttaagtacgggggtgtccttacacacgccggcagaaagcaggctgaggaactgggtagatattttcggaataacatgtatccaggtgaaggtactggcctgcttcgcctccatagcacctatcgccatgatcttaaaatctacagctctgatgagggccgtgtgcagatgtctgcagctgcatttgccaaaggccttcttgatctagaagggcagctaacaccaatcctggtttctcttgttagcaaggactcctctatgttggatggacttgaaaatgcaagtgttgagatggaggaagcaaaggctaggttgaacgagattataacttcaggtgtaaagacagctcaaagcaatggaatatctccttggatggcagatggagggggactgcctccaaatgctttggagcttctacctgacttggtaaagttaactaagaaggtcactgaacaagtaagacagctagccaaggatgaagatgaggaacttatgacaagctcatatgatgtgatacttccttatgaccaagcaaaggcccttggtaagactaatattgacgtggatcgtattgctgctggattaccctgtggtagcgagggattcctgttgatgtatgctcgttggagaaagcttgtaagggacttgtacaatgagcgaaaagagcgattcgacataacgcagattccagatgtttatgactcctgcaaatatgatcttctacacaatgggcatctgaatttagacggtctagatgagctcttcaaagttggccagttacttgctgatggtgtcatcccaaacgagtatggcattaatccaacacagaagctcaagattggttccaagatcgctcgtcgtttactgggtaaaataatgattgatctgaggaatactcgtgaagaagccatcagcattgcagaaccaaagagcactcaggatgaacctttgaagttaacaatttcggagaaggacgataaggaataccacacaaagcttaatgtgaagaatgatgacagaaaatgtagcacaagtgatgtcccaaagcttcacaacaagaatgaagatatgagaagatctagtatatcaagtgagatatcaatggatcaggaggatgatgatgataaagagatcaaatatcgtttggacccgaagtatgcaaatgtcagaacacctgaacgtcatgttagaacacgcctttactttacatcagaatctcatatccattctctaatgaatgttctccgctactgcaacctggatgaatctcttcaaggagaggatggccttgtttgtgaaagtgctttagagcgtttatacaacactaaagagcttgactacatgagttatatagtgctgaggatgttcgagaacacagaagtagccttagaagacccaaaaagatttcgtgtagaaatgacctttagtcgtggtgcagatctatcgccattgaaggaaaatgacagtgaggctgcttcattgcatcaagagcacacactgccgataatgggtccagagaggctgcaagaagtgggatcatatctcacattcgataaaatggagaaaatgattcggtcatttgctatgccagcggaagattttcctccaccatcaactccagcaggattctcgggctatttctcgaagagtgcagcagtgctagagcgcctggtaaatctctggcctttcaagcatgctagttctaatggaaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000827 GO:0000828 GO:0000829 GO:0000832 GO:0002376 GO:0002682 GO:0002684 GO:0002831 GO:0002833 GO:0003674 GO:0003824 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005975 GO:0006020 GO:0006066 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0006955 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009814 GO:0009817 GO:0009861 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0017076 GO:0019222 GO:0019637 GO:0019751 GO:0030554 GO:0030656 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032101 GO:0032103 GO:0032553 GO:0032555 GO:0032559 GO:0032958 GO:0033857 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043207 GO:0043647 GO:0043900 GO:0043902 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046136 GO:0046165 GO:0046173 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0062012 GO:0062013 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0090407 GO:0097159 GO:0097367 GO:0098542 GO:1900150 GO:1900367 GO:1901265 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1904965 GO:1904966 GO:1905034 GO:1905036 GO:2000068
KEGG Pathways
Metabolic & Signaling
ko04070 map04070
Pfam Domains
Protein Families
His_Phos_2

Protein Analysis

1406

Amino Acids

159.95

Weight (kDa)

6.11

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2831
AarI CACCTGC 2 cut(s) 485, 1529
Acc16I TGCGCA 2 cut(s) 657, 1701
Acc36I ACCTGC 2 cut(s) 485, 1529
AccII CGCG 1 cut(s) 56
AciI CCGC 7 cut(s) 54, 263, 797, 1841, 2432, 3751, 4097
AclI AACGTT 2 cut(s) 418, 1462
AclWI GGATC 4 cut(s) 134, 3078, 3618, 4048
AcoI YGGCCR 2 cut(s) 155, 3277
AcsI RAATTY 9 cut(s) 89, 98, 318, 599, 874, 1362, 1643, 1918, 3244
AcuI CTGAAG 6 cut(s) 68, 234, 1145, 2189, 2456, 2820
AfaI GTAC 9 cut(s) 635, 1166, 1251, 1679, 2210, 2295, 2513, 2599, 3155
AflII CTTAAG 1 cut(s) 2507
AflIII ACRYGT 1 cut(s) 2580
AhdI GACNNNNNGTC 1 cut(s) 3930
AjiI CACGTC 1 cut(s) 3067
AjnI CCWGG 6 cut(s) 1264, 2308, 2587, 2733, 3762, 4173
AjuI GAANNNNNNNTTGG 8 cut(s) 985, 1017, 2029, 2061, 2722, 2754, 2857, 2889
AloI GAACNNNNNNTCC 4 cut(s) 309, 341, 1353, 1385
Alw21I GWGCWC 7 cut(s) 372, 1338, 1416, 2382, 3267, 3440, 3999
Alw26I GTCTC 4 cut(s) 565, 1184, 1609, 2228
AlwI GGATC 4 cut(s) 134, 3078, 3618, 4048
Ama87I CYCGRG 4 cut(s) 74, 1337, 2381, 4135
ApaI GGGCCC 2 cut(s) 647, 1691
ApeKI GCWGC 9 cut(s) 899, 1943, 2681, 2684, 2719, 3080, 3977, 4027, 4157
ApoI RAATTY 9 cut(s) 89, 98, 318, 599, 874, 1362, 1643, 1918, 3244
AseI ATTAAT 1 cut(s) 3321
Asp700I GAANNNNTTC 7 cut(s) 47, 1143, 1314, 2187, 2358, 3774, 4104
AspLEI GCGC 5 cut(s) 58, 81, 658, 1702, 4173
AsuHPI GGTGA 8 cut(s) 131, 571, 1106, 1615, 2150, 2477, 2483, 2603
AsuNHI GCTAGC 1 cut(s) 2971
AvaI CYCGRG 4 cut(s) 74, 1337, 2381, 4135
AvaII GGWCC 2 cut(s) 3652, 4016
BaeGI GKGCMC 2 cut(s) 647, 1691
BalI TGGCCA 1 cut(s) 3279
BanII GRGCYC 5 cut(s) 372, 647, 1416, 1691, 3267
BarI GAAGNNNNNNTAC 4 cut(s) 863, 895, 1907, 1939
BauI CACGAG 1 cut(s) 3402
BbsI GAAGAC 3 cut(s) 1299, 2343, 3903
Bbv12I GWGCWC 7 cut(s) 372, 1338, 1416, 2382, 3267, 3440, 3999
BbvCI CCTCAGC 6 cut(s) 663, 1056, 1707, 2100, 2550, 3863
BbvI GCAGC 9 cut(s) 886, 1930, 2671, 2693, 2731, 3067, 3964, 4014, 4169
BceAI ACGGC 1 cut(s) 2649
BcgI CGANNNNNNTGC 4 cut(s) 3930, 3964, 4116, 4150
BciT130I CCWGG 6 cut(s) 1266, 2310, 2589, 2735, 3764, 4175
BciVI GTATCC 1 cut(s) 2595
BclI TGATCA 2 cut(s) 460, 1504
BcoDI GTCTC 4 cut(s) 565, 1184, 1609, 2228
BfmI CTRYAG 2 cut(s) 2647, 2679
BfoI RGCGCY 2 cut(s) 82, 4174
BfrI CTTAAG 1 cut(s) 2507
BfuAI ACCTGC 2 cut(s) 485, 1529
BfuI GTATCC 1 cut(s) 2595
BglI GCCNNNNNGGC 1 cut(s) 64
BglII AGATCT 4 cut(s) 1184, 2228, 3581, 3943
Bme1390I CCNGG 6 cut(s) 1266, 2310, 2589, 2735, 3764, 4175
Bme18I GGWCC 2 cut(s) 3652, 4016
BmeRI GACNNNNNGTC 1 cut(s) 3930
BmeT110I CYCGRG 4 cut(s) 74, 1337, 2381, 4135
BmgBI CACGTC 1 cut(s) 3067
BmiI GGNNCC 8 cut(s) 261, 645, 1175, 1689, 2219, 3349, 3654, 4017
BmrFI CCNGG 6 cut(s) 1266, 2310, 2589, 2735, 3764, 4175
BmrI ACTGGG 2 cut(s) 2567, 3380
BmtI GCTAGC 1 cut(s) 2975
BmuI ACTGGG 2 cut(s) 2567, 3380
BpiI GAAGAC 3 cut(s) 1299, 2343, 3903
BplI GAGNNNNNCTC 6 cut(s) 818, 850, 1862, 1894, 3385, 3417
BpmI CTGGAG 3 cut(s) 633, 1677, 4107
Bpu10I CCTNAGC 6 cut(s) 663, 1056, 1707, 2100, 2550, 3863
BpuEI CTTGAG 1 cut(s) 3323
BsaJI CCNNGG 9 cut(s) 974, 1021, 1134, 2018, 2065, 2178, 2873, 2976, 3046
BsaWI WCCGGW 1 cut(s) 142
Bse118I RCCGGY 1 cut(s) 2534
Bse1I ACTGG 6 cut(s) 616, 1660, 2562, 2605, 3280, 3375
Bse3DI GCAATG 5 cut(s) 780, 1824, 2866, 3420, 3983
BseBI CCWGG 6 cut(s) 1266, 2310, 2589, 2735, 3764, 4175
BseDI CCNNGG 9 cut(s) 974, 1021, 1134, 2018, 2065, 2178, 2873, 2976, 3046
BseMI GCAATG 5 cut(s) 780, 1824, 2866, 3420, 3983
BseNI ACTGG 6 cut(s) 616, 1660, 2562, 2605, 3280, 3375
BseRI GAGGAG 5 cut(s) 930, 1355, 1974, 2399, 2749
BseSI GKGCMC 2 cut(s) 647, 1691
BseXI GCAGC 9 cut(s) 886, 1930, 2671, 2693, 2731, 3067, 3964, 4014, 4169
BsgI GTGCAG 3 cut(s) 2690, 3960, 4176
Bsh1236I CGCG 1 cut(s) 56
BsiHKAI GWGCWC 7 cut(s) 372, 1338, 1416, 2382, 3267, 3440, 3999
BsiHKCI CYCGRG 4 cut(s) 74, 1337, 2381, 4135
BsiSI CCGG 2 cut(s) 143, 2535
BslFI GGGAC 6 cut(s) 843, 1887, 2406, 2907, 3161, 3530
BsmAI GTCTC 4 cut(s) 565, 1184, 1609, 2228
BsmBI CGTCTC 2 cut(s) 565, 1609
BsmFI GGGAC 6 cut(s) 843, 1887, 2406, 2907, 3161, 3530
BsoBI CYCGRG 4 cut(s) 74, 1337, 2381, 4135
Bsp120I GGGCCC 2 cut(s) 643, 1687
Bsp1286I GDGCHC 9 cut(s) 372, 647, 1338, 1416, 1691, 2382, 3267, 3440, 3999
Bsp1407I TGTACA 3 cut(s) 633, 1677, 3153
Bsp19I CCATGG 2 cut(s) 974, 2018
BspACI CCGC 7 cut(s) 54, 263, 797, 1841, 2432, 3751, 4097
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 3 cut(s) 277, 699, 1743
BspLI GGNNCC 8 cut(s) 261, 645, 1175, 1689, 2219, 3349, 3654, 4017
BspMAI CTGCAG 1 cut(s) 2683
BspMI ACCTGC 2 cut(s) 485, 1529
BspOI GCTAGC 1 cut(s) 2975
BspPI GGATC 4 cut(s) 134, 3078, 3618, 4048
BspQI GCTCTTC 1 cut(s) 3272
BspTI CTTAAG 1 cut(s) 2507
BsrDI GCAATG 5 cut(s) 780, 1824, 2866, 3420, 3983
BsrFI RCCGGY 1 cut(s) 2534
BsrGI TGTACA 3 cut(s) 633, 1677, 3153
BsrI ACTGG 6 cut(s) 616, 1660, 2562, 2605, 3280, 3375
BssAI RCCGGY 1 cut(s) 2534
BssECI CCNNGG 9 cut(s) 974, 1021, 1134, 2018, 2065, 2178, 2873, 2976, 3046
BssSI CACGAG 1 cut(s) 3402
BssT1I CCWWGG 7 cut(s) 974, 1021, 2018, 2065, 2873, 2976, 3046
Bst2BI CACGAG 1 cut(s) 3402
Bst2UI CCWGG 6 cut(s) 1266, 2310, 2589, 2735, 3764, 4175
Bst4CI ACNGT 8 cut(s) 467, 640, 1283, 1511, 1684, 2327, 3254, 3971
Bst6I CTCTTC 5 cut(s) 559, 1603, 3272, 3780, 4145
BstAFI CTTAAG 1 cut(s) 2507
BstAPI GCANNNNNTGC 1 cut(s) 4003
BstAUI TGTACA 3 cut(s) 633, 1677, 3153
BstDSI CCRYGG 2 cut(s) 974, 2018
BstEII GGTNACC 4 cut(s) 530, 1129, 1574, 2173
BstFNI CGCG 1 cut(s) 56
BstH2I RGCGCY 2 cut(s) 82, 4174
BstHHI GCGC 5 cut(s) 58, 81, 658, 1702, 4173
BstMAI GTCTC 4 cut(s) 565, 1184, 1609, 2228
BstNI CCWGG 6 cut(s) 1266, 2310, 2589, 2735, 3764, 4175
BstNSI RCATGY 6 cut(s) 662, 1307, 1706, 2351, 2584, 4202
BstPI GGTNACC 4 cut(s) 530, 1129, 1574, 2173
BstSCI CCNGG 6 cut(s) 1264, 2308, 2587, 2733, 3762, 4173
BstSFI CTRYAG 2 cut(s) 2647, 2679
BstSLI GKGCMC 2 cut(s) 647, 1691
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 9 cut(s) 886, 1930, 2671, 2693, 2731, 3067, 3964, 4014, 4169
BstV2I GAAGAC 3 cut(s) 1299, 2343, 3903
BstX2I RGATCY 5 cut(s) 139, 1184, 2228, 3581, 3943
BstXI CCANNNNNNTGG 3 cut(s) 191, 1028, 2072
BstYI RGATCY 5 cut(s) 139, 1184, 2228, 3581, 3943
BsuI GTATCC 1 cut(s) 2595
BtgI CCRYGG 2 cut(s) 974, 2018
BtrI CACGTC 1 cut(s) 3067
BtsI GCAGTG 4 cut(s) 1338, 2382, 4001, 4167
BtsIMutI CAGTG 8 cut(s) 1288, 1338, 2332, 2382, 2952, 3976, 4001, 4167
BveI ACCTGC 2 cut(s) 485, 1529
CciI TCATGA 3 cut(s) 277, 699, 1743
CfoI GCGC 5 cut(s) 58, 81, 658, 1702, 4173
Cfr10I RCCGGY 1 cut(s) 2534
CseI GACGC 4 cut(s) 290, 840, 1334, 1884
CsiI ACCWGGT 2 cut(s) 1264, 2308
Csp6I GTAC 9 cut(s) 634, 1165, 1250, 1678, 2209, 2294, 2512, 2598, 3154
CviQI GTAC 9 cut(s) 634, 1165, 1250, 1678, 2209, 2294, 2512, 2598, 3154
DriI GACNNNNNGTC 1 cut(s) 3930
EaeI YGGCCR 2 cut(s) 155, 3277
Eam1104I CTCTTC 5 cut(s) 559, 1603, 3272, 3780, 4145
Eam1105I GACNNNNNGTC 1 cut(s) 3930
EarI CTCTTC 5 cut(s) 559, 1603, 3272, 3780, 4145
Ecl136II GAGCTC 3 cut(s) 370, 1414, 3265
Eco130I CCWWGG 7 cut(s) 974, 1021, 2018, 2065, 2873, 2976, 3046
Eco147I AGGCCT 1 cut(s) 2700
Eco24I GRGCYC 5 cut(s) 372, 647, 1416, 1691, 3267
Eco32I GATATC 3 cut(s) 731, 1775, 3603
Eco47I GGWCC 2 cut(s) 3652, 4016
Eco53kI GAGCTC 3 cut(s) 370, 1414, 3265
Eco57I CTGAAG 6 cut(s) 68, 234, 1145, 2189, 2456, 2820
Eco88I CYCGRG 4 cut(s) 74, 1337, 2381, 4135
Eco91I GGTNACC 4 cut(s) 530, 1129, 1574, 2173
EcoICRI GAGCTC 3 cut(s) 370, 1414, 3265
EcoO109I RGGNCCY 3 cut(s) 644, 1688, 3043
EcoO65I GGTNACC 4 cut(s) 530, 1129, 1574, 2173
EcoRI GAATTC 7 cut(s) 98, 318, 599, 874, 1362, 1643, 1918
EcoRII CCWGG 6 cut(s) 1264, 2308, 2587, 2733, 3762, 4173
EcoRV GATATC 3 cut(s) 731, 1775, 3603
EcoT14I CCWWGG 7 cut(s) 974, 1021, 2018, 2065, 2873, 2976, 3046
EcoT38I GRGCYC 5 cut(s) 372, 647, 1416, 1691, 3267
ErhI CCWWGG 7 cut(s) 974, 1021, 2018, 2065, 2873, 2976, 3046
Esp3I CGTCTC 2 cut(s) 565, 1609
FalI AAGNNNNNCTT 8 cut(s) 223, 255, 985, 1017, 2029, 2061, 2688, 2720
FaqI GGGAC 6 cut(s) 843, 1887, 2406, 2907, 3161, 3530
FauNDI CATATG 2 cut(s) 220, 3010
FbaI TGATCA 2 cut(s) 460, 1504
FriOI GRGCYC 5 cut(s) 372, 647, 1416, 1691, 3267
FspAI RTGCGCAY 2 cut(s) 657, 1701
FspI TGCGCA 2 cut(s) 657, 1701
GlaI GCGC 5 cut(s) 57, 80, 657, 1701, 4172
GsuI CTGGAG 3 cut(s) 633, 1677, 4107
HaeII RGCGCY 2 cut(s) 82, 4174
HapII CCGG 2 cut(s) 143, 2535
HgaI GACGC 4 cut(s) 290, 840, 1334, 1884
HhaI GCGC 5 cut(s) 58, 81, 658, 1702, 4173
Hin6I GCGC 5 cut(s) 56, 79, 656, 1700, 4171
HinP1I GCGC 5 cut(s) 56, 79, 656, 1700, 4171
HincII GTYRAC 4 cut(s) 416, 1460, 2940, 3462
HindII GTYRAC 4 cut(s) 416, 1460, 2940, 3462
HindIII AAGCTT 8 cut(s) 1142, 1313, 2186, 2357, 2493, 3137, 3500, 3551
HpaI GTTAAC 2 cut(s) 2940, 3462
HpaII CCGG 2 cut(s) 143, 2535
HphI GGTGA 8 cut(s) 131, 571, 1106, 1615, 2150, 2477, 2483, 2603
Hpy99I CGWCG 1 cut(s) 3366
HpyCH4III ACNGT 8 cut(s) 467, 640, 1283, 1511, 1684, 2327, 3254, 3971
HpyCH4IV ACGT 8 cut(s) 418, 820, 1066, 1462, 1864, 2110, 3066, 3685
HpySE526I ACGT 8 cut(s) 418, 820, 1066, 1462, 1864, 2110, 3066, 3685
HspAI GCGC 5 cut(s) 56, 79, 656, 1700, 4171
KroI GCCGGC 1 cut(s) 2534
KroNI GCCGGC 1 cut(s) 2536
Ksp22I TGATCA 2 cut(s) 460, 1504
KspAI GTTAAC 2 cut(s) 2940, 3462
LguI GCTCTTC 1 cut(s) 3272
LmnI GCTCC 7 cut(s) 259, 839, 943, 1342, 1883, 1987, 2914
Lsp1109I GCAGC 9 cut(s) 886, 1930, 2671, 2693, 2731, 3067, 3964, 4014, 4169
MabI ACCWGGT 2 cut(s) 1264, 2308
MaeII ACGT 8 cut(s) 418, 820, 1066, 1462, 1864, 2110, 3066, 3685
MaeIII GTNAC 9 cut(s) 119, 530, 1129, 1277, 1574, 2173, 2321, 2950, 3282
MfeI CAATTG 2 cut(s) 1214, 2258
MflI RGATCY 5 cut(s) 139, 1184, 2228, 3581, 3943
MhlI GDGCHC 9 cut(s) 372, 647, 1338, 1416, 1691, 2382, 3267, 3440, 3999
MlsI TGGCCA 1 cut(s) 3279
MluNI TGGCCA 1 cut(s) 3279
MlyI GAGTC 2 cut(s) 2750, 3200
MmeI TCCRAC 5 cut(s) 237, 1209, 2748, 3110, 3350
Mox20I TGGCCA 1 cut(s) 3279
MroNI GCCGGC 1 cut(s) 2534
MroXI GAANNNNTTC 7 cut(s) 47, 1143, 1314, 2187, 2358, 3774, 4104
MscI TGGCCA 1 cut(s) 3279
MslI CAYNNNNRTG 2 cut(s) 117, 1241
Msp20I TGGCCA 1 cut(s) 3279
MspA1I CMGCKG 3 cut(s) 2434, 2684, 4097
MspCI CTTAAG 1 cut(s) 2507
MspI CCGG 2 cut(s) 143, 2535
MspR9I CCNGG 6 cut(s) 1266, 2310, 2589, 2735, 3764, 4175
MunI CAATTG 2 cut(s) 1214, 2258
MvaI CCWGG 6 cut(s) 1266, 2310, 2589, 2735, 3764, 4175
MvnI CGCG 1 cut(s) 56
NaeI GCCGGC 1 cut(s) 2536
NcoI CCATGG 2 cut(s) 974, 2018
NdeI CATATG 2 cut(s) 220, 3010
NgoMIV GCCGGC 1 cut(s) 2534
NheI GCTAGC 1 cut(s) 2971
NlaIV GGNNCC 8 cut(s) 261, 645, 1175, 1689, 2219, 3349, 3654, 4017
NmeAIII GCCGAG 2 cut(s) 470, 1514
NmuCI GTSAC 4 cut(s) 119, 1277, 2321, 2950
NsbI TGCGCA 2 cut(s) 657, 1701
NspI RCATGY 6 cut(s) 662, 1307, 1706, 2351, 2584, 4202
PaeI GCATGC 5 cut(s) 662, 1307, 1706, 2351, 4202
PaeR7I CTCGAG 3 cut(s) 74, 1337, 2381
PagI TCATGA 3 cut(s) 277, 699, 1743
PaqCI CACCTGC 2 cut(s) 485, 1529
PceI AGGCCT 1 cut(s) 2700
PciI ACATGT 1 cut(s) 2580
PciSI GCTCTTC 1 cut(s) 3272
PdiI GCCGGC 1 cut(s) 2536
PdmI GAANNNNTTC 7 cut(s) 47, 1143, 1314, 2187, 2358, 3774, 4104
PflFI GACNNNGTC 3 cut(s) 125, 1266, 2310
PleI GAGTC 2 cut(s) 2750, 3200
PpsI GAGTC 2 cut(s) 2750, 3200
PscI ACATGT 1 cut(s) 2580
PshBI ATTAAT 1 cut(s) 3321
PsiI TTATAA 1 cut(s) 2831
Psp124BI GAGCTC 3 cut(s) 372, 1416, 3267
Psp1406I AACGTT 2 cut(s) 418, 1462
Psp6I CCWGG 6 cut(s) 1264, 2308, 2587, 2733, 3762, 4173
PspEI GGTNACC 4 cut(s) 530, 1129, 1574, 2173
PspGI CCWGG 6 cut(s) 1264, 2308, 2587, 2733, 3762, 4173
PspN4I GGNNCC 8 cut(s) 261, 645, 1175, 1689, 2219, 3349, 3654, 4017
PspOMI GGGCCC 2 cut(s) 643, 1687
PspXI VCTCGAGB 2 cut(s) 1337, 2381
PstI CTGCAG 1 cut(s) 2683
PsuI RGATCY 5 cut(s) 139, 1184, 2228, 3581, 3943
PsyI GACNNNGTC 3 cut(s) 125, 1266, 2310
PvuII CAGCTG 1 cut(s) 2684
RsaI GTAC 9 cut(s) 635, 1166, 1251, 1679, 2210, 2295, 2513, 2599, 3155
RsaNI GTAC 9 cut(s) 634, 1165, 1250, 1678, 2209, 2294, 2512, 2598, 3154
RseI CAYNNNNRTG 2 cut(s) 117, 1241
SacI GAGCTC 3 cut(s) 372, 1416, 3267
SapI GCTCTTC 1 cut(s) 3272
SchI GAGTC 2 cut(s) 2750, 3200
ScrFI CCNGG 6 cut(s) 1266, 2310, 2589, 2735, 3764, 4175
SduI GDGCHC 9 cut(s) 372, 647, 1338, 1416, 1691, 2382, 3267, 3440, 3999
SexAI ACCWGGT 2 cut(s) 1264, 2308
SfcI CTRYAG 2 cut(s) 2647, 2679
Sfr274I CTCGAG 3 cut(s) 74, 1337, 2381
SinI GGWCC 2 cut(s) 3652, 4016
SlaI CTCGAG 3 cut(s) 74, 1337, 2381
SmiMI CAYNNNNRTG 2 cut(s) 117, 1241
SmlI CTYRAG 5 cut(s) 74, 1337, 2381, 2507, 3338
SmoI CTYRAG 5 cut(s) 74, 1337, 2381, 2507, 3338
SphI GCATGC 5 cut(s) 662, 1307, 1706, 2351, 4202
SseBI AGGCCT 1 cut(s) 2700
SsiI CCGC 7 cut(s) 54, 263, 797, 1841, 2432, 3751, 4097
SspI AATATT 1 cut(s) 3061
SstI GAGCTC 3 cut(s) 372, 1416, 3267
StuI AGGCCT 1 cut(s) 2700
StyD4I CCNGG 6 cut(s) 1264, 2308, 2587, 2733, 3762, 4173
StyI CCWWGG 7 cut(s) 974, 1021, 2018, 2065, 2873, 2976, 3046
TaaI ACNGT 8 cut(s) 467, 640, 1283, 1511, 1684, 2327, 3254, 3971
TaiI ACGT 8 cut(s) 421, 823, 1069, 1465, 1867, 2113, 3069, 3688
TaqII GACCGA 2 cut(s) 297, 4068
TatI WGTACW 5 cut(s) 633, 1164, 1677, 2208, 3153
TauI GCSGC 2 cut(s) 265, 2434
TscAI CASTG 8 cut(s) 1288, 1338, 2332, 2382, 2959, 3976, 4008, 4167
TseFI GTSAC 4 cut(s) 119, 1277, 2321, 2950
TseI GCWGC 9 cut(s) 899, 1943, 2681, 2684, 2719, 3080, 3977, 4027, 4157
Tsp45I GTSAC 4 cut(s) 119, 1277, 2321, 2950
TspGWI ACGGA 4 cut(s) 767, 1255, 1811, 2299
TspRI CASTG 8 cut(s) 1288, 1338, 2332, 2382, 2959, 3976, 4008, 4167
Tth111I GACNNNGTC 3 cut(s) 125, 1266, 2310
Vha464I CTTAAG 1 cut(s) 2507
VpaK11BI GGWCC 2 cut(s) 3652, 4016
VspI ATTAAT 1 cut(s) 3321
XapI RAATTY 9 cut(s) 89, 98, 318, 599, 874, 1362, 1643, 1918, 3244
XbaI TCTAGA 4 cut(s) 1259, 2303, 2710, 3256
XceI RCATGY 6 cut(s) 662, 1307, 1706, 2351, 2584, 4202
XcmI CCANNNNNNNNNTGG 2 cut(s) 972, 2016
XhoI CTCGAG 3 cut(s) 74, 1337, 2381
XmnI GAANNNNTTC 7 cut(s) 47, 1143, 1314, 2187, 2358, 3774, 4104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.