Rroxscaffold_2G00082320

Belongs to the histidine acid phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004152
Physical Location & Seq
Forward (+)
5192389 .. 5204125
11737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00082320.1

Sequence Viewer

Length: 3201 bp
ATGGAGAAGAAGATTAAGATTGGGGTTTGTGTGATGGAAAAGAAGGTGAAATGCGGCTCCGAGGTGTTCTCGGCGCCGATGGGGCAGATTCTCGAGCGCCTTCAGGAATTTGGCGAATTTGAGATTACACATTTCGGTGACAAGGTCATTCTGGAAGATCCGGTTGAGAGCTGGCCGATTTGTGATTGCTTGATTGCCTTCCATTCCTCTGGCTATCCTCTGGAAAAGGCTGAAGCATATGCTAATTTAAGGAAACCTTTTCTCGTAAACGAGTTGGAGCCACAACACCTTCTTCATGACCGAAGGAAGGTATATGAGCGTCTTGAAATGTTTGGAATTCCTGTTCCGAGATATGCCCTTGTAAATAGAGAAGCTCCATATCAAGAGCTTGACTATTTTGTTGAGGAAGAAGATTTTGTTGAGGTTCATGGTCAACGTTTCTGGAAGCCATTTGTGGAGAAGCCTGTTGAGGGGGATGATCACAGTATAATGATATATTACCCTAGCTCGGCAGGTGGAGGAATGAAGGAGTTGTTTAGGAAGGTTGGTAACCGATCTAGTGAGTTCCATCCAGAGGTGAGAAGAGTGAGACGAGAAGGCTCCTATATATATGAGGAATTCATGCCAACTGGAGGCACAGATGTCAAGGTGTACACGGTGGGCCCTGAATATGCGCATGCTGAGGCAAGGAAATCTCCCGTTGTAGATGGGGTTGTCATGAGAAATCCTGATGGCAAGGAAGTGAGATATCCTGTTTTACTGACACCTACGGAGAAGCAAATGGCAAGGGAGGTCTGCATTGCATTTAGGCAAGCGGTTTGTGGGTTCGATCTTTTACGTTGTGAGGGACGCTCCTATGTCTGTGATGTGAATGGATGGAGTTTCGTGAAGAATTCCTACAAGTATTATGATGATGCTGCTTGTGTGCTCAGGAAGATATTTATAGATGCTAAAGCTCCTCATCTTTCTTCTGTAATTCCACCAACTTTACCATGGAAAATCAATGAACCAAGCCAACCTTCTGAGGGGCTAACTCGCCAAGGAAGTGGAATCATTGGCACATTTGGGCAGGCTGAGGAACTACGTTGCGTAATTGCCATTATTCGTCATGGTGATAGAACTCCCAAACAGAAAGTAAAGCTGAAGGTTACCGAGGAAAAGCTTCTAAACTTGATGCTAAAGTACAATGGGGGCCGACCTAGATCTGAGACAAAACTGAAAAGTGCTATCCAATTGCAAGATTTGTTGGATGCCACACGGATGCTTGTACCTCGTTCTAGACCAGGTCGAGAAAGTGACAGTGAGGCAGAAGACATTGAGCATGCTGAAAAGCTTCGTCAAGTTAAAGCAGTGCTTGAGGAGGGGGGACACTTCTCTGGCATTTATAGGAAAGTTCAGCTAAAGCCGCTGAAGTGGGATAAAGTTGCTAAAAGTAATGGTGAAGGTGAGGAAGAACGCCCTGTTGAAGCTTTGATGGTTCTTAAGTACGGGGGTGTCCTTACACACGCCGGCAGAAAGCAGGCTGAGGAACTGGGTAGATATTTTCGGAATAACATGTATCCAGGTGAAGGTACTGGCCTGCTTCGCCTTCATAGCACCTATCGCCATGATCTTAAAATCTACAGCTCTGATGAGGGCCGTGTGCAGATGTCTGCAGCTGCATTTGCCAAAGGCCTACTTGATCTAGAAGGGCAGCTAACACCAATCCTGGTTTCTCTTGTTAGCAAGGACTCCTCTATGTTGGATGGACTTGAAAATGCAAGTGTTGAGATGGAGGAAGCAAAGGCTAGGTTGAACGAGATTATAACTTCAGGTGTAAAGACAGCTCAAAGCAATGGAATACCTCCTTGGATGGCTGATGGAGGGGGACTGCCACCAAATGCTTTGGAGCTTCTACCTGACTTGGTAAAGTTAACTAAGAAGGTCACTGAACAAGTAAGACTGCTAGCCAAGGATGAAGATGAGGAACTTATGACAAGCTCATATGACGTGATACTTCCTTATGACCAAGCAAAGGCCCTTGGTAAGACTAACATTGACGTTGATCGTATTGCTGCTGGATTACCCTGTGGTAGCGAGGGATTCCTGTTGATGTATGCTCGTTGGAGAAAGCTTGTAAGGGACTTGTACAATGAACGAAAAGAACGGTTCGACATAACGCAGATCCCAGATGTTTATGACTCCTGCAAATATGATCTTCTACACAATGGGCATCTGAATTTAGACGGTCTAGATGAGCTCTTCAAAGTTGCCCAGTTACTTGCTGATGGTGTCATCCCAAATGAGTATGGCATCAATCCAACACAGAAGCTCAAGATTGGTTCGAAGATTGCTCGTCGTTTACTGGGTAAAATAATGATTGATCTGAGGAATACTCGTGAAGAAGCCATCAGCATTGCAGAACCAAAGAGTACTCAGGATGAACCTTTGAAGTTAACAATTTCGGAGAAGGACGATAAGGAATACCACACAAAGCTTAATGTGAAGAATGATGACAGAAAATGTAGCACAAGTGATGTACCAAAGCTTCACAATAAGAATGAAGATATGAGGAGATCTAGTATTTCAAGTGAGATATCAATGGATCAGGAGGATGATGATGATAAAGAGATCAAATATCGTTTGGACCCGAAGTATGCAAATGTCAGAACTCCTGAACGTCATGTTCGAACACGCCTTTACTTTACATCAGAATCTCACATTCATTCTCTAATGAATGTTCTCCGCTACTGCAACCTGGATGAATCTCTTCAAGGAGAGGATGGCCTTATTTGTGAAAGTGCTTTAGAGCGTTTATACAACACTAAAGAGCTTGACTACATGAGTTATATAGTGCTGAGGATGTTCGAGAACACAGAACAGGTAGCCTTAGAAGACCCAAAAAGATTTCGTGTAGAAATGACCTTTAGTCGTGGTGCAGATCTATCGCCATTGAAGAAAAATGACAGTGAGGCGGCTTCGTTGCATCAAGAGCACACACTACCGATAATGGGTCCAGAGAGGCTGCAAGAAGTGGGATCGTATCTCACATTCGATAAAATGGAGAAAATGATTCGGTCATTTGCTATGCCAGCGGAAGATTTTCCTCCACCATCAACTCCAGCAGGATTCTCGGGCTATTTCTCGAAGAGTGCAGCAGTGCTGGAGCGCCTGGTAAATCTCTGGCCTTTCCATAAGCATGCTAGTTCTAATGGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000827 GO:0000828 GO:0000829 GO:0000832 GO:0002376 GO:0002682 GO:0002684 GO:0002831 GO:0002833 GO:0003674 GO:0003824 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005975 GO:0006020 GO:0006066 GO:0006793 GO:0006796 GO:0006950 GO:0006952 GO:0006955 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009814 GO:0009817 GO:0009861 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0017076 GO:0019222 GO:0019637 GO:0019751 GO:0030554 GO:0030656 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032101 GO:0032103 GO:0032553 GO:0032555 GO:0032559 GO:0032958 GO:0033857 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043207 GO:0043647 GO:0043900 GO:0043902 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046136 GO:0046165 GO:0046173 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0062012 GO:0062013 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0090407 GO:0097159 GO:0097367 GO:0098542 GO:1900150 GO:1900367 GO:1901265 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1904965 GO:1904966 GO:1905034 GO:1905036 GO:2000068
KEGG Pathways
Metabolic & Signaling
ko04070 map04070
Pfam Domains
Protein Families
His_Phos_2

Protein Analysis

1066

Amino Acids

121.16

Weight (kDa)

5.96

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPIP5K2_N PF18086 5 - 100 3.4e-36 Diphosphoinositol pentakisphosphate kinase 2 N-terminal domain
His_Phos_2 PF00328 360 - 969 6.6e-70 Histidine phosphatase superfamily (branch 2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1805
AarI CACCTGC 1 cut(s) 503
Acc16I TGCGCA 1 cut(s) 675
Acc36I ACCTGC 1 cut(s) 503
AccB1I GGYRCC 1 cut(s) 73
AciI CCGC 6 cut(s) 54, 815, 1406, 2725, 2954, 3074
AclI AACGTT 1 cut(s) 436
AclWI GGATC 4 cut(s) 152, 2158, 2592, 3025
AcoI YGGCCR 1 cut(s) 173
AcsI RAATTY 6 cut(s) 107, 116, 336, 617, 892, 2218
AcuI CTGAAG 5 cut(s) 86, 252, 1163, 1430, 1794
AcyI GRCGYC 1 cut(s) 74
AfaI GTAC 8 cut(s) 653, 1184, 1269, 1487, 1573, 2129, 2413, 2520
AfiI CCNNNNNNNGG 9 cut(s) 307, 470, 508, 574, 632, 1025, 1568, 2014, 2990
AflII CTTAAG 1 cut(s) 1481
AflIII ACRYGT 1 cut(s) 1554
AgsI TTSAA 9 cut(s) 326, 1466, 1754, 1795, 2245, 2431, 2568, 2753, 2935
AhdI GACNNNNNGTC 1 cut(s) 2907
AjiI CACGTC 1 cut(s) 1990
AjnI CCWGG 5 cut(s) 1282, 1561, 1707, 2736, 3150
AjuI GAANNNNNNNTTGG 6 cut(s) 1003, 1035, 1696, 1728, 1831, 1863
AloI GAACNNNNNNTCC 2 cut(s) 327, 359
Alw21I GWGCWC 3 cut(s) 930, 2241, 2976
Alw26I GTCTC 2 cut(s) 583, 1202
AlwI GGATC 4 cut(s) 152, 2158, 2592, 3025
Ama87I CYCGRG 2 cut(s) 92, 3112
AoxI GGCC 9 cut(s) 173, 661, 1192, 1576, 1636, 1672, 2016, 2764, 3164
ApaI GGGCCC 1 cut(s) 665
ApeKI GCWGC 7 cut(s) 917, 1655, 1658, 1693, 2054, 3004, 3134
ApoI RAATTY 6 cut(s) 107, 116, 336, 617, 892, 2218
Asp700I GAANNNNTTC 4 cut(s) 1161, 1332, 2748, 3081
AspLEI GCGC 4 cut(s) 76, 99, 676, 3150
AspS9I GGNCC 7 cut(s) 661, 662, 1192, 1636, 2017, 2626, 2993
AsuHPI GGTGA 7 cut(s) 58, 149, 589, 1124, 1451, 1457, 1577
AsuII TTCGAA 2 cut(s) 2324, 2668
AsuNHI GCTAGC 1 cut(s) 1945
AvaI CYCGRG 2 cut(s) 92, 3112
AvaII GGWCC 2 cut(s) 2626, 2993
BaeGI GKGCMC 1 cut(s) 665
BanI GGYRCC 1 cut(s) 73
BanII GRGCYC 2 cut(s) 665, 2241
BarI GAAGNNNNNNTAC 4 cut(s) 881, 913, 2511, 2543
BauI CACGAG 1 cut(s) 2376
BbsI GAAGAC 2 cut(s) 1317, 2880
Bbv12I GWGCWC 3 cut(s) 930, 2241, 2976
BbvCI CCTCAGC 4 cut(s) 681, 1074, 1524, 2837
BbvI GCAGC 7 cut(s) 904, 1645, 1667, 1705, 2041, 2991, 3146
BceAI ACGGC 1 cut(s) 1623
BcgI CGANNNNNNTGC 4 cut(s) 2907, 2941, 3093, 3127
BciT130I CCWGG 5 cut(s) 1284, 1563, 1709, 2738, 3152
BciVI GTATCC 1 cut(s) 1569
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 583, 1202
BfmI CTRYAG 2 cut(s) 1621, 1653
BfoI RGCGCY 3 cut(s) 77, 100, 3151
BfrI CTTAAG 1 cut(s) 1481
BfuAI ACCTGC 1 cut(s) 503
BfuI GTATCC 1 cut(s) 1569
BglI GCCNNNNNGGC 1 cut(s) 82
BglII AGATCT 3 cut(s) 1202, 2555, 2920
BmcAI AGTACT 1 cut(s) 2413
Bme1390I CCNGG 5 cut(s) 1284, 1563, 1709, 2738, 3152
Bme18I GGWCC 2 cut(s) 2626, 2993
BmeRI GACNNNNNGTC 1 cut(s) 2907
BmeT110I CYCGRG 2 cut(s) 92, 3112
BmgBI CACGTC 1 cut(s) 1990
BmgT120I GGNCC 7 cut(s) 661, 662, 1192, 1636, 2017, 2626, 2993
BmiI GGNNCC 8 cut(s) 58, 75, 279, 601, 663, 1193, 2628, 2994
BmrFI CCNGG 5 cut(s) 1284, 1563, 1709, 2738, 3152
BmrI ACTGGG 3 cut(s) 1541, 2248, 2354
BmsI GCATC 8 cut(s) 904, 937, 1164, 1240, 1251, 2221, 2301, 2974
BmtI GCTAGC 1 cut(s) 1949
BmuI ACTGGG 3 cut(s) 1541, 2248, 2354
BpiI GAAGAC 2 cut(s) 1317, 2880
BplI GAGNNNNNCTC 6 cut(s) 53, 85, 836, 868, 2359, 2391
BpmI CTGGAG 3 cut(s) 651, 3084, 3164
Bpu10I CCTNAGC 5 cut(s) 681, 929, 1074, 1524, 2837
Bpu14I TTCGAA 2 cut(s) 2324, 2668
BpuEI CTTGAG 2 cut(s) 1376, 2297
BsaHI GRCGYC 1 cut(s) 74
BsaJI CCNNGG 7 cut(s) 60, 992, 1039, 1152, 1847, 1950, 2020
BsaWI WCCGGW 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 2545, 2575
Bsc4I CCNNNNNNNGG 9 cut(s) 307, 470, 508, 574, 632, 1025, 1568, 2014, 2990
Bse118I RCCGGY 1 cut(s) 1508
Bse1I ACTGG 5 cut(s) 634, 1536, 1579, 2254, 2349
Bse3DI GCAATG 3 cut(s) 798, 1840, 2394
BseBI CCWGG 5 cut(s) 1284, 1563, 1709, 2738, 3152
BseDI CCNNGG 7 cut(s) 60, 992, 1039, 1152, 1847, 1950, 2020
BseLI CCNNNNNNNGG 9 cut(s) 307, 470, 508, 574, 632, 1025, 1568, 2014, 2990
BseMI GCAATG 3 cut(s) 798, 1840, 2394
BseMII CTCAG 9 cut(s) 672, 943, 1014, 1065, 1197, 1515, 2357, 2429, 2828
BseNI ACTGG 5 cut(s) 634, 1536, 1579, 2254, 2349
BseRI GAGGAG 4 cut(s) 948, 1373, 1723, 2566
BseSI GKGCMC 1 cut(s) 665
BseXI GCAGC 7 cut(s) 904, 1645, 1667, 1705, 2041, 2991, 3146
BsgI GTGCAG 3 cut(s) 1664, 2937, 3153
BshFI GGCC 9 cut(s) 175, 663, 1194, 1578, 1638, 1674, 2018, 2766, 3166
BshNI GGYRCC 1 cut(s) 73
BsiHKAI GWGCWC 3 cut(s) 930, 2241, 2976
BsiHKCI CYCGRG 2 cut(s) 92, 3112
BsiSI CCGG 2 cut(s) 161, 1509
BslFI GGGAC 4 cut(s) 861, 1380, 1881, 2135
BslI CCNNNNNNNGG 9 cut(s) 307, 470, 508, 574, 632, 1025, 1568, 2014, 2990
BsmAI GTCTC 2 cut(s) 583, 1202
BsmBI CGTCTC 1 cut(s) 583
BsmFI GGGAC 4 cut(s) 861, 1380, 1881, 2135
BsnI GGCC 9 cut(s) 175, 663, 1194, 1578, 1638, 1674, 2018, 2766, 3166
BsoBI CYCGRG 2 cut(s) 92, 3112
Bsp119I TTCGAA 2 cut(s) 2324, 2668
Bsp120I GGGCCC 1 cut(s) 661
Bsp1286I GDGCHC 4 cut(s) 665, 930, 2241, 2976
Bsp1407I TGTACA 2 cut(s) 651, 2127
Bsp19I CCATGG 1 cut(s) 992
BspACI CCGC 6 cut(s) 54, 815, 1406, 2725, 2954, 3074
BspANI GGCC 9 cut(s) 175, 663, 1194, 1578, 1638, 1674, 2018, 2766, 3166
BspCNI CTCAG 9 cut(s) 673, 942, 1015, 1066, 1198, 1516, 2358, 2428, 2829
BspHI TCATGA 2 cut(s) 295, 717
BspLI GGNNCC 8 cut(s) 58, 75, 279, 601, 663, 1193, 2628, 2994
BspMAI CTGCAG 1 cut(s) 1657
BspMI ACCTGC 1 cut(s) 503
BspOI GCTAGC 1 cut(s) 1949
BspPI GGATC 4 cut(s) 152, 2158, 2592, 3025
BspQI GCTCTTC 1 cut(s) 2246
BspT104I TTCGAA 2 cut(s) 2324, 2668
BspT107I GGYRCC 1 cut(s) 73
BspTI CTTAAG 1 cut(s) 1481
BsrDI GCAATG 3 cut(s) 798, 1840, 2394
BsrFI RCCGGY 1 cut(s) 1508
BsrGI TGTACA 2 cut(s) 651, 2127
BsrI ACTGG 5 cut(s) 634, 1536, 1579, 2254, 2349
BssAI RCCGGY 1 cut(s) 1508
BssECI CCNNGG 7 cut(s) 60, 992, 1039, 1152, 1847, 1950, 2020
BssNI GRCGYC 1 cut(s) 74
BssSI CACGAG 1 cut(s) 2376
BssT1I CCWWGG 5 cut(s) 992, 1039, 1847, 1950, 2020
Bst2BI CACGAG 1 cut(s) 2376
Bst2UI CCWGG 5 cut(s) 1284, 1563, 1709, 2738, 3152
Bst4CI ACNGT 6 cut(s) 485, 658, 1301, 2148, 2228, 2948
Bst6I CTCTTC 4 cut(s) 577, 2246, 2754, 3122
BstACI GRCGYC 1 cut(s) 74
BstAFI CTTAAG 1 cut(s) 1481
BstAUI TGTACA 2 cut(s) 651, 2127
BstBI TTCGAA 2 cut(s) 2324, 2668
BstDSI CCRYGG 1 cut(s) 992
BstEII GGTNACC 2 cut(s) 548, 1147
BstH2I RGCGCY 3 cut(s) 77, 100, 3151
BstHHI GCGC 4 cut(s) 76, 99, 676, 3150
BstMAI GTCTC 2 cut(s) 583, 1202
BstMWI GCNNNNNNNGC 8 cut(s) 82, 1405, 1584, 1593, 1602, 1664, 2971, 3071
BstNI CCWGG 5 cut(s) 1284, 1563, 1709, 2738, 3152
BstNSI RCATGY 4 cut(s) 680, 1325, 1558, 3182
BstPI GGTNACC 2 cut(s) 548, 1147
BstSCI CCNGG 5 cut(s) 1282, 1561, 1707, 2736, 3150
BstSFI CTRYAG 2 cut(s) 1621, 1653
BstSLI GKGCMC 1 cut(s) 665
BstV1I GCAGC 7 cut(s) 904, 1645, 1667, 1705, 2041, 2991, 3146
BstV2I GAAGAC 2 cut(s) 1317, 2880
BstX2I RGATCY 5 cut(s) 157, 1202, 2163, 2555, 2920
BstXI CCANNNNNNTGG 2 cut(s) 209, 1046
BstYI RGATCY 5 cut(s) 157, 1202, 2163, 2555, 2920
BsuI GTATCC 1 cut(s) 1569
BsuRI GGCC 9 cut(s) 175, 663, 1194, 1578, 1638, 1674, 2018, 2766, 3166
BtgI CCRYGG 1 cut(s) 992
BtrI CACGTC 1 cut(s) 1990
BtsI GCAGTG 2 cut(s) 1356, 3144
BtsIMutI CAGTG 5 cut(s) 1306, 1356, 1926, 2953, 3144
BveI ACCTGC 1 cut(s) 503
CciI TCATGA 2 cut(s) 295, 717
CfoI GCGC 4 cut(s) 76, 99, 676, 3150
Cfr10I RCCGGY 1 cut(s) 1508
Cfr13I GGNCC 7 cut(s) 661, 662, 1192, 1636, 2017, 2626, 2993
CseI GACGC 2 cut(s) 308, 858
CsiI ACCWGGT 1 cut(s) 1282
Csp6I GTAC 8 cut(s) 652, 1183, 1268, 1486, 1572, 2128, 2412, 2519
CviQI GTAC 8 cut(s) 652, 1183, 1268, 1486, 1572, 2128, 2412, 2519
DinI GGCGCC 1 cut(s) 75
DriI GACNNNNNGTC 1 cut(s) 2907
EaeI YGGCCR 1 cut(s) 173
Eam1104I CTCTTC 4 cut(s) 577, 2246, 2754, 3122
Eam1105I GACNNNNNGTC 1 cut(s) 2907
EarI CTCTTC 4 cut(s) 577, 2246, 2754, 3122
Ecl136II GAGCTC 1 cut(s) 2239
Eco130I CCWWGG 5 cut(s) 992, 1039, 1847, 1950, 2020
Eco147I AGGCCT 1 cut(s) 1674
Eco24I GRGCYC 2 cut(s) 665, 2241
Eco32I GATATC 2 cut(s) 749, 2577
Eco47I GGWCC 2 cut(s) 2626, 2993
Eco53kI GAGCTC 1 cut(s) 2239
Eco57I CTGAAG 5 cut(s) 86, 252, 1163, 1430, 1794
Eco88I CYCGRG 2 cut(s) 92, 3112
Eco91I GGTNACC 2 cut(s) 548, 1147
EcoICRI GAGCTC 1 cut(s) 2239
EcoO109I RGGNCCY 2 cut(s) 662, 2017
EcoO65I GGTNACC 2 cut(s) 548, 1147
EcoRI GAATTC 3 cut(s) 336, 617, 892
EcoRII CCWGG 5 cut(s) 1282, 1561, 1707, 2736, 3150
EcoRV GATATC 2 cut(s) 749, 2577
EcoT14I CCWWGG 5 cut(s) 992, 1039, 1847, 1950, 2020
EcoT38I GRGCYC 2 cut(s) 665, 2241
EgeI GGCGCC 1 cut(s) 75
EheI GGCGCC 1 cut(s) 75
ErhI CCWWGG 5 cut(s) 992, 1039, 1847, 1950, 2020
Esp3I CGTCTC 1 cut(s) 583
FalI AAGNNNNNCTT 8 cut(s) 241, 273, 1003, 1035, 1338, 1370, 1662, 1694
FaqI GGGAC 4 cut(s) 861, 1380, 1881, 2135
FauNDI CATATG 2 cut(s) 238, 1984
FbaI TGATCA 1 cut(s) 478
FriOI GRGCYC 2 cut(s) 665, 2241
FspAI RTGCGCAY 1 cut(s) 675
FspI TGCGCA 1 cut(s) 675
GlaI GCGC 4 cut(s) 75, 98, 675, 3149
GsuI CTGGAG 3 cut(s) 651, 3084, 3164
HaeII RGCGCY 3 cut(s) 77, 100, 3151
HaeIII GGCC 9 cut(s) 175, 663, 1194, 1578, 1638, 1674, 2018, 2766, 3166
HapII CCGG 2 cut(s) 161, 1509
HgaI GACGC 2 cut(s) 308, 858
HhaI GCGC 4 cut(s) 76, 99, 676, 3150
Hin1I GRCGYC 1 cut(s) 74
Hin6I GCGC 4 cut(s) 74, 97, 674, 3148
HinP1I GCGC 4 cut(s) 74, 97, 674, 3148
HincII GTYRAC 3 cut(s) 434, 1914, 2436
HindII GTYRAC 3 cut(s) 434, 1914, 2436
HindIII AAGCTT 6 cut(s) 1160, 1331, 1467, 2111, 2474, 2525
HinfI GANTC 9 cut(s) 88, 1050, 1730, 2082, 2180, 2693, 2744, 3052, 3108
HpaI GTTAAC 2 cut(s) 1914, 2436
HpaII CCGG 2 cut(s) 161, 1509
HphI GGTGA 7 cut(s) 58, 149, 589, 1124, 1451, 1457, 1577
Hpy166II GTNNAC 7 cut(s) 268, 434, 652, 654, 1914, 2342, 2436
Hpy8I GTNNAC 7 cut(s) 268, 434, 652, 654, 1914, 2342, 2436
Hpy99I CGWCG 1 cut(s) 2340
HpyCH4III ACNGT 6 cut(s) 485, 658, 1301, 2148, 2228, 2948
HpyCH4IV ACGT 6 cut(s) 436, 838, 1084, 1989, 2040, 2659
HpyF10VI GCNNNNNNNGC 8 cut(s) 82, 1405, 1584, 1593, 1602, 1664, 2971, 3071
HpySE526I ACGT 6 cut(s) 436, 838, 1084, 1989, 2040, 2659
Hsp92I GRCGYC 1 cut(s) 74
HspAI GCGC 4 cut(s) 74, 97, 674, 3148
KasI GGCGCC 1 cut(s) 73
KroI GCCGGC 1 cut(s) 1508
KroNI GCCGGC 1 cut(s) 1510
Ksp22I TGATCA 1 cut(s) 478
KspAI GTTAAC 2 cut(s) 1914, 2436
LguI GCTCTTC 1 cut(s) 2246
LmnI GCTCC 8 cut(s) 62, 277, 379, 605, 857, 961, 1888, 3145
Lsp1109I GCAGC 7 cut(s) 904, 1645, 1667, 1705, 2041, 2991, 3146
LweI GCATC 8 cut(s) 904, 937, 1164, 1240, 1251, 2221, 2301, 2974
MabI ACCWGGT 1 cut(s) 1282
MaeII ACGT 6 cut(s) 436, 838, 1084, 1989, 2040, 2659
MaeIII GTNAC 6 cut(s) 137, 548, 1147, 1295, 1924, 2256
MfeI CAATTG 1 cut(s) 1232
MflI RGATCY 5 cut(s) 157, 1202, 2163, 2555, 2920
MhlI GDGCHC 4 cut(s) 665, 930, 2241, 2976
Mly113I GGCGCC 1 cut(s) 74
MlyI GAGTC 2 cut(s) 1724, 2174
MmeI TCCRAC 5 cut(s) 255, 1227, 1722, 2084, 2324
MroNI GCCGGC 1 cut(s) 1508
MroXI GAANNNNTTC 4 cut(s) 1161, 1332, 2748, 3081
MseI TTAA 8 cut(s) 15, 248, 1344, 1482, 1614, 1913, 2435, 2478
MslI CAYNNNNRTG 3 cut(s) 135, 1259, 3177
MspA1I CMGCKG 3 cut(s) 1408, 1658, 3074
MspCI CTTAAG 1 cut(s) 1481
MspI CCGG 2 cut(s) 161, 1509
MspR9I CCNGG 5 cut(s) 1284, 1563, 1709, 2738, 3152
MunI CAATTG 1 cut(s) 1232
MvaI CCWGG 5 cut(s) 1284, 1563, 1709, 2738, 3152
MwoI GCNNNNNNNGC 8 cut(s) 82, 1405, 1584, 1593, 1602, 1664, 2971, 3071
NaeI GCCGGC 1 cut(s) 1510
NarI GGCGCC 1 cut(s) 74
NcoI CCATGG 1 cut(s) 992
NdeI CATATG 2 cut(s) 238, 1984
NgoMIV GCCGGC 1 cut(s) 1508
NheI GCTAGC 1 cut(s) 1945
NlaIV GGNNCC 8 cut(s) 58, 75, 279, 601, 663, 1193, 2628, 2994
NmeAIII GCCGAG 2 cut(s) 50, 488
NmuCI GTSAC 3 cut(s) 137, 1295, 1924
NsbI TGCGCA 1 cut(s) 675
NspI RCATGY 4 cut(s) 680, 1325, 1558, 3182
NspV TTCGAA 2 cut(s) 2324, 2668
PaeI GCATGC 3 cut(s) 680, 1325, 3182
PaeR7I CTCGAG 1 cut(s) 92
PagI TCATGA 2 cut(s) 295, 717
PaqCI CACCTGC 1 cut(s) 503
PceI AGGCCT 1 cut(s) 1674
PciI ACATGT 1 cut(s) 1554
PciSI GCTCTTC 1 cut(s) 2246
PcsI WCGNNNNNNNCGW 1 cut(s) 2665
PdiI GCCGGC 1 cut(s) 1510
PdmI GAANNNNTTC 4 cut(s) 1161, 1332, 2748, 3081
PfeI GAWTC 7 cut(s) 88, 1050, 2082, 2693, 2744, 3052, 3108
PflFI GACNNNGTC 2 cut(s) 143, 1284
PleI GAGTC 2 cut(s) 1724, 2174
PluTI GGCGCC 1 cut(s) 77
PpsI GAGTC 2 cut(s) 1724, 2174
PscI ACATGT 1 cut(s) 1554
PsiI TTATAA 1 cut(s) 1805
Psp124BI GAGCTC 1 cut(s) 2241
Psp1406I AACGTT 1 cut(s) 436
Psp6I CCWGG 5 cut(s) 1282, 1561, 1707, 2736, 3150
PspEI GGTNACC 2 cut(s) 548, 1147
PspGI CCWGG 5 cut(s) 1282, 1561, 1707, 2736, 3150
PspN4I GGNNCC 8 cut(s) 58, 75, 279, 601, 663, 1193, 2628, 2994
PspOMI GGGCCC 1 cut(s) 661
PspPI GGNCC 7 cut(s) 661, 662, 1192, 1636, 2017, 2626, 2993
PstI CTGCAG 1 cut(s) 1657
PsuI RGATCY 5 cut(s) 157, 1202, 2163, 2555, 2920
PsyI GACNNNGTC 2 cut(s) 143, 1284
PvuII CAGCTG 1 cut(s) 1658
RsaI GTAC 8 cut(s) 653, 1184, 1269, 1487, 1573, 2129, 2413, 2520
RsaNI GTAC 8 cut(s) 652, 1183, 1268, 1486, 1572, 2128, 2412, 2519
RseI CAYNNNNRTG 3 cut(s) 135, 1259, 3177
SacI GAGCTC 1 cut(s) 2241
SapI GCTCTTC 1 cut(s) 2246
SaqAI TTAA 8 cut(s) 15, 248, 1344, 1482, 1614, 1913, 2435, 2478
Sau96I GGNCC 7 cut(s) 661, 662, 1192, 1636, 2017, 2626, 2993
ScaI AGTACT 1 cut(s) 2413
SchI GAGTC 2 cut(s) 1724, 2174
ScrFI CCNGG 5 cut(s) 1284, 1563, 1709, 2738, 3152
SduI GDGCHC 4 cut(s) 665, 930, 2241, 2976
SexAI ACCWGGT 1 cut(s) 1282
SfaNI GCATC 8 cut(s) 904, 937, 1164, 1240, 1251, 2221, 2301, 2974
SfcI CTRYAG 2 cut(s) 1621, 1653
SfoI GGCGCC 1 cut(s) 75
Sfr274I CTCGAG 1 cut(s) 92
SfuI TTCGAA 2 cut(s) 2324, 2668
SinI GGWCC 2 cut(s) 2626, 2993
SlaI CTCGAG 1 cut(s) 92
SmiMI CAYNNNNRTG 3 cut(s) 135, 1259, 3177
SmlI CTYRAG 4 cut(s) 92, 1355, 1481, 2312
SmoI CTYRAG 4 cut(s) 92, 1355, 1481, 2312
SphI GCATGC 3 cut(s) 680, 1325, 3182
SseBI AGGCCT 1 cut(s) 1674
SsiI CCGC 6 cut(s) 54, 815, 1406, 2725, 2954, 3074
SspDI GGCGCC 1 cut(s) 73
SstI GAGCTC 1 cut(s) 2241
StuI AGGCCT 1 cut(s) 1674
StyD4I CCNGG 5 cut(s) 1282, 1561, 1707, 2736, 3150
StyI CCWWGG 5 cut(s) 992, 1039, 1847, 1950, 2020
TaaI ACNGT 6 cut(s) 485, 658, 1301, 2148, 2228, 2948
TaiI ACGT 6 cut(s) 439, 841, 1087, 1992, 2043, 2662
TaqI TCGA 9 cut(s) 93, 828, 1288, 2151, 2324, 2668, 2847, 3033, 3125
TaqII GACCGA 2 cut(s) 315, 3045
TatI WGTACW 4 cut(s) 651, 1182, 2127, 2411
TauI GCSGC 3 cut(s) 57, 1408, 2957
TfiI GAWTC 7 cut(s) 88, 1050, 2082, 2693, 2744, 3052, 3108
Tru1I TTAA 8 cut(s) 15, 248, 1344, 1482, 1614, 1913, 2435, 2478
Tru9I TTAA 8 cut(s) 15, 248, 1344, 1482, 1614, 1913, 2435, 2478
TscAI CASTG 5 cut(s) 1306, 1356, 1933, 2953, 3144
TseFI GTSAC 3 cut(s) 137, 1295, 1924
TseI GCWGC 7 cut(s) 917, 1655, 1658, 1693, 2054, 3004, 3134
Tsp45I GTSAC 3 cut(s) 137, 1295, 1924
TspGWI ACGGA 2 cut(s) 785, 1273
TspRI CASTG 5 cut(s) 1306, 1356, 1933, 2953, 3144
Tth111I GACNNNGTC 2 cut(s) 143, 1284
Vha464I CTTAAG 1 cut(s) 1481
VpaK11BI GGWCC 2 cut(s) 2626, 2993
XapI RAATTY 6 cut(s) 107, 116, 336, 617, 892, 2218
XbaI TCTAGA 3 cut(s) 1277, 1684, 2230
XceI RCATGY 4 cut(s) 680, 1325, 1558, 3182
XcmI CCANNNNNNNNNTGG 1 cut(s) 990
XhoI CTCGAG 1 cut(s) 92
XmnI GAANNNNTTC 4 cut(s) 1161, 1332, 2748, 3081
ZrmI AGTACT 1 cut(s) 2413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.