pycom06g10970

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0018715
Physical Location & Seq
Forward (+)
16172466 .. 16172837
372 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g10970.1

Sequence Viewer

Length: 372 bp
ATGGCGATTCTAAGCGTTCAAGGCTTCTCGGCGCTGCCTCAGCATTGCCTACAGCTCTGCTACGGCTTTTTCGCCTTCGCGGTTTTAGTTAACCTAGTCAGAGACTTTTCGCCTAAGATTGGAAAATGGATGCCACTTCCGATGGTCATGGGCGTTCCCTTTCTGGTCGGGGCATACTTTGCCATAGACATGTGCATTGGGAGTCTGATTGTGTTTGTATGGCACAAGCTTAACAGCAAGAAGGCCGTGCTAATGGTTCCCGCGGTTGCTTCCGGATTGATTTGCGGTGAAGGGTTGTGGACTCTTCCCGCTTCCATTCTTGCTCTGGCCAAAGTAAAACCCCCAATGTGCATGAAATTTTTGGGTTCCTAG

Protein Analysis

124

Amino Acids

13.32

Weight (kDa)

9.3

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPT PF03169 2 - 107 2.6e-17 OPT oligopeptide transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018715)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45450
fragaria_vesca FvH4_7g32520
malus_domestica MD14G1126100.v1.1
pyrus_communis pycom06g10970
rosa_chinensis RchiOBHm_Chr2g0168901
rosa_multiflora Rmu_co8144192.1_g000001 Rmu_sc0001035.1_g000057 Rmu_sc0026870.1_g000001
rosa_rugosa Rorug05G0225400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 80, 263
AccIII TCCGGA 1 cut(s) 272
AciI CCGC 5 cut(s) 80, 261, 263, 285, 309
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 119
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 1 cut(s) 20
AluBI AGCT 2 cut(s) 55, 229
AluI AGCT 2 cut(s) 55, 229
Alw26I GTCTC 1 cut(s) 96
Aor13HI TCCGGA 1 cut(s) 272
AoxI GGCC 2 cut(s) 243, 327
ApeKI GCWGC 1 cut(s) 34
ApoI RAATTY 1 cut(s) 356
AspLEI GCGC 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 299
BalI TGGCCA 1 cut(s) 329
BbvCI CCTCAGC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 21
BccI CCATC 1 cut(s) 136
BceAI ACGGC 2 cut(s) 79, 230
BcoDI GTCTC 1 cut(s) 96
BfaI CTAG 2 cut(s) 95, 370
BfmI CTRYAG 1 cut(s) 50
BfoI RGCGCY 1 cut(s) 35
BisI GCNGC 1 cut(s) 35
BlsI GCNGC 1 cut(s) 36
BmiI GGNNCC 2 cut(s) 258, 367
BmsI GCATC 1 cut(s) 120
Bpu10I CCTNAGC 1 cut(s) 39
BsaJI CCNNGG 1 cut(s) 261
BsaWI WCCGGW 1 cut(s) 272
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse3DI GCAATG 1 cut(s) 43
BseAI TCCGGA 1 cut(s) 272
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 1 cut(s) 135
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 43
BseMII CTCAG 1 cut(s) 53
BseXI GCAGC 1 cut(s) 21
Bsh1236I CGCG 2 cut(s) 80, 263
BshFI GGCC 2 cut(s) 245, 329
BsiSI CCGG 1 cut(s) 273
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 96
BsnI GGCC 2 cut(s) 245, 329
Bsp13I TCCGGA 1 cut(s) 272
BspACI CCGC 5 cut(s) 80, 261, 263, 285, 309
BspANI GGCC 2 cut(s) 245, 329
BspCNI CTCAG 1 cut(s) 52
BspEI TCCGGA 1 cut(s) 272
BspFNI CGCG 2 cut(s) 80, 263
BspLI GGNNCC 2 cut(s) 258, 367
BsrDI GCAATG 1 cut(s) 43
BssECI CCNNGG 1 cut(s) 261
Bst6I CTCTTC 1 cut(s) 309
BstAPI GCANNNNNTGC 1 cut(s) 179
BstDEI CTNAG 3 cut(s) 11, 39, 114
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 1 cut(s) 135
BstFNI CGCG 2 cut(s) 80, 263
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 1 cut(s) 34
BstMAI GTCTC 1 cut(s) 96
BstMWI GCNNNNNNNGC 3 cut(s) 21, 40, 179
BstNSI RCATGY 1 cut(s) 193
BstSFI CTRYAG 1 cut(s) 50
BstUI CGCG 2 cut(s) 80, 263
BstV1I GCAGC 1 cut(s) 21
BsuRI GGCC 2 cut(s) 245, 329
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 1 cut(s) 135
CfoI GCGC 1 cut(s) 34
Cfr42I CCGCGG 1 cut(s) 264
CviAII CATG 3 cut(s) 148, 190, 352
CviJI RGCY 6 cut(s) 24, 55, 66, 229, 245, 329
CviKI_1 RGCY 6 cut(s) 24, 55, 66, 229, 245, 329
DdeI CTNAG 3 cut(s) 11, 39, 114
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
FaeI CATG 3 cut(s) 151, 193, 355
FaiI YATR 6 cut(s) 149, 175, 185, 191, 220, 353
FatI CATG 3 cut(s) 147, 189, 351
FauI CCCGC 2 cut(s) 268, 316
Fnu4HI GCNGC 1 cut(s) 35
FokI GGATG 1 cut(s) 142
Fsp4HI GCNGC 1 cut(s) 35
FspBI CTAG 2 cut(s) 95, 370
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 35
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 2 cut(s) 245, 329
HapII CCGG 1 cut(s) 273
HhaI GCGC 1 cut(s) 34
Hin1II CATG 3 cut(s) 151, 193, 355
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 3 cut(s) 7, 202, 301
HpaI GTTAAC 1 cut(s) 91
HpaII CCGG 1 cut(s) 273
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 2 cut(s) 91, 300
Hpy188I TCNGA 3 cut(s) 101, 141, 207
Hpy188III TCNNGA 1 cut(s) 273
Hpy8I GTNNAC 2 cut(s) 91, 300
HpyAV CCTTC 3 cut(s) 85, 235, 284
HpyCH4V TGCA 2 cut(s) 195, 351
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 40, 179
HpyF3I CTNAG 3 cut(s) 11, 39, 114
Hsp92II CATG 3 cut(s) 151, 193, 355
HspAI GCGC 1 cut(s) 32
Kpn2I TCCGGA 1 cut(s) 272
KspAI GTTAAC 1 cut(s) 91
KspI CCGCGG 1 cut(s) 264
LpnPI CCDG 3 cut(s) 149, 286, 311
Lsp1109I GCAGC 1 cut(s) 21
LweI GCATC 1 cut(s) 120
MaeI CTAG 2 cut(s) 95, 370
MboII GAAGA 1 cut(s) 296
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 1 cut(s) 356
MluNI TGGCCA 1 cut(s) 329
MlyI GAGTC 2 cut(s) 211, 295
MnlI CCTC 1 cut(s) 48
Mox20I TGGCCA 1 cut(s) 329
MroI TCCGGA 1 cut(s) 272
MscI TGGCCA 1 cut(s) 329
MseI TTAA 2 cut(s) 90, 231
MslI CAYNNNNRTG 1 cut(s) 188
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 1 cut(s) 273
MvnI CGCG 2 cut(s) 80, 263
MwoI GCNNNNNNNGC 3 cut(s) 21, 40, 179
NlaIII CATG 3 cut(s) 151, 193, 355
NlaIV GGNNCC 2 cut(s) 258, 367
NmeAIII GCCGAG 1 cut(s) 8
NspI RCATGY 1 cut(s) 193
PciI ACATGT 1 cut(s) 189
PfeI GAWTC 1 cut(s) 7
PkrI GCNGC 1 cut(s) 36
PleI GAGTC 2 cut(s) 210, 295
PpsI GAGTC 2 cut(s) 210, 295
PscI ACATGT 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 258, 367
RseI CAYNNNNRTG 1 cut(s) 188
SacII CCGCGG 1 cut(s) 264
SaqAI TTAA 2 cut(s) 90, 231
SatI GCNGC 1 cut(s) 35
SchI GAGTC 2 cut(s) 211, 295
SetI ASST 3 cut(s) 57, 96, 231
SfaNI GCATC 1 cut(s) 120
SfcI CTRYAG 1 cut(s) 50
Sfr303I CCGCGG 1 cut(s) 264
SgrBI CCGCGG 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 188
Sse9I AATT 1 cut(s) 356
SsiI CCGC 5 cut(s) 80, 261, 263, 285, 309
SspMI CTAG 2 cut(s) 95, 370
TasI AATT 1 cut(s) 356
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 2 cut(s) 90, 231
Tru9I TTAA 2 cut(s) 90, 231
TseI GCWGC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 368
XapI RAATTY 1 cut(s) 356
XceI RCATGY 1 cut(s) 193
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XspI CTAG 2 cut(s) 95, 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.