Rorug05G0225400

metal-nicotianamine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0018715
Physical Location & Seq
Forward (+)
23407399 .. 23408437
1039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0225400.1

Sequence Viewer

Length: 825 bp
ATGGTTTGGAAGAAGGAATACTTGGATTTAATCTTGGTCCCAACTGGGTTGCTAATCATGTTGTGCTACCATCTCTTTCTCCTCTACAGATGCCTACGGCATCCTGAAACCACAGTCATTGGCAATGAAAACCACTGCAGAAATGCTTGGGTTGAGAGAATGTTGCAGATTGAAGCAAAGGATCGAGGTCAGTCCCTAGCAGTAATCTCCAGCACTATAACAGCAGCAAATTTCTTGGCTTCAACCTCTTTAGCCTTGAGTTCTTTGATTGGAACGTGGGTTCTAAACTCTTCAAACAGCATCACTAGTTTTACGTATGGTGACACAAGTCCTGCAATGAATTCTATTAAATACGTTAGCATTCTGGCCTGCTTCCTATTAGCTCTAGCTTCTTTCTTACACTGTATAAGAAGCTTTATCCATGCCACTTTCCTCATTAGCATGCCACACAGTGATACTCCTCTGAGTGATGTTCAGAAGGCTGTGATACATGGCGGCCTTTTTTGGACATTGGGGTTGAGAGCAATCTATTTTGCCACTAATTTACTCCTCTGGGTTTTTGGTCCAATTCCGATGTTTGTGTGTTCAGTGACTATGGTGTTGATTCTGAACAAGCTGGATTCGAATTCCACTCCATTGCATCAGTTCCAACCAGCCAGGAGTCATGACAATAGGTTGATGGGGAAACTTGCTGAAGAAAAAACCCAAATACATAGGGTTATCGAGCAACACGAAAGATCACACATAGATGATAGCAAAACTCAAGGTTCTAACTGTCAACAATCACCTGCCTCTAGTAAAGATTTTTCAGAGGCAACAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPT

Protein Analysis

274

Amino Acids

30.62

Weight (kDa)

7.69

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 211 1.3e-57 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018715)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45450
fragaria_vesca FvH4_7g32520
malus_domestica MD14G1126100.v1.1
pyrus_communis pycom06g10970
rosa_chinensis RchiOBHm_Chr2g0168901
rosa_multiflora Rmu_co8144192.1_g000001 Rmu_sc0001035.1_g000057 Rmu_sc0026870.1_g000001
rosa_rugosa Rorug05G0225400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 796
Acc36I ACCTGC 1 cut(s) 796
AciI CCGC 1 cut(s) 495
AclWI GGATC 1 cut(s) 189
AcsI RAATTY 3 cut(s) 229, 340, 625
AcuI CTGAAG 1 cut(s) 714
AdeI CACNNNGTG 1 cut(s) 452
AgsI TTSAA 3 cut(s) 173, 243, 294
AhlI ACTAGT 1 cut(s) 305
AjnI CCWGG 1 cut(s) 656
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AloI GAACNNNNNNTCC 2 cut(s) 264, 296
AluBI AGCT 4 cut(s) 383, 389, 414, 616
AluI AGCT 4 cut(s) 383, 389, 414, 616
AlwI GGATC 1 cut(s) 189
AoxI GGCC 2 cut(s) 366, 496
ApeKI GCWGC 1 cut(s) 224
ApoI RAATTY 3 cut(s) 229, 340, 625
AspS9I GGNCC 2 cut(s) 37, 563
AsuHPI GGTGA 2 cut(s) 332, 777
AsuII TTCGAA 1 cut(s) 623
AvaII GGWCC 2 cut(s) 37, 563
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbvI GCAGC 1 cut(s) 236
BccI CCATC 2 cut(s) 78, 673
BceAI ACGGC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 658
BcuI ACTAGT 1 cut(s) 305
BfaI CTAG 4 cut(s) 197, 306, 386, 795
BfmI CTRYAG 2 cut(s) 85, 136
BfuAI ACCTGC 1 cut(s) 796
BisI GCNGC 2 cut(s) 225, 496
BlsI GCNGC 2 cut(s) 226, 497
Bme1390I CCNGG 1 cut(s) 658
Bme18I GGWCC 2 cut(s) 37, 563
BmgT120I GGNCC 2 cut(s) 37, 563
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 658
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 4 cut(s) 80, 109, 309, 649
BmuI ACTGGG 1 cut(s) 54
BoxI GACNNNNGTC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 193
Bpu14I TTCGAA 1 cut(s) 623
BpuEI CTTGAG 2 cut(s) 277, 747
BsaAI YACGTR 1 cut(s) 315
Bse1I ACTGG 1 cut(s) 49
Bse3DI GCAATG 3 cut(s) 130, 342, 635
BseBI CCWGG 1 cut(s) 658
BseGI GGATG 1 cut(s) 100
BseMI GCAATG 3 cut(s) 130, 342, 635
BseMII CTCAG 1 cut(s) 455
BseNI ACTGG 1 cut(s) 49
BseRI GAGGAG 3 cut(s) 71, 450, 539
BseXI GCAGC 1 cut(s) 236
BshFI GGCC 2 cut(s) 368, 498
BslFI GGGAC 2 cut(s) 23, 178
BsmFI GGGAC 2 cut(s) 23, 178
BsmI GAATGC 1 cut(s) 360
BsnI GGCC 2 cut(s) 368, 498
Bsp119I TTCGAA 1 cut(s) 623
Bsp143I GATC 2 cut(s) 181, 737
BspACI CCGC 1 cut(s) 495
BspANI GGCC 2 cut(s) 368, 498
BspCNI CTCAG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 1 cut(s) 39
BspMAI CTGCAG 1 cut(s) 140
BspMI ACCTGC 1 cut(s) 796
BspPI GGATC 1 cut(s) 189
BspT104I TTCGAA 1 cut(s) 623
BsrDI GCAATG 3 cut(s) 130, 342, 635
BsrI ACTGG 1 cut(s) 49
BssMI GATC 2 cut(s) 181, 737
Bst2UI CCWGG 1 cut(s) 658
Bst4CI ACNGT 4 cut(s) 115, 404, 452, 776
Bst6I CTCTTC 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 315
BstBI TTCGAA 1 cut(s) 623
BstC8I GCNNGC 2 cut(s) 370, 443
BstDEI CTNAG 1 cut(s) 464
BstF5I GGATG 1 cut(s) 100
BstKTI GATC 2 cut(s) 184, 740
BstMBI GATC 2 cut(s) 181, 737
BstNI CCWGG 1 cut(s) 658
BstNSI RCATGY 1 cut(s) 445
BstPAI GACNNNNGTC 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 656
BstSFI CTRYAG 2 cut(s) 85, 136
BstSNI TACGTA 1 cut(s) 315
BstV1I GCAGC 1 cut(s) 236
BsuRI GGCC 2 cut(s) 368, 498
BtsCI GGATG 1 cut(s) 100
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 4 cut(s) 133, 400, 457, 594
BveI ACCTGC 1 cut(s) 796
Cac8I GCNNGC 2 cut(s) 370, 443
CciI TCATGA 1 cut(s) 664
Cfr13I GGNCC 2 cut(s) 37, 563
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 5 cut(s) 58, 422, 442, 491, 665
DdeI CTNAG 1 cut(s) 464
DpnI GATC 2 cut(s) 183, 739
DpnII GATC 2 cut(s) 181, 737
DraIII CACNNNGTG 1 cut(s) 452
Eam1104I CTCTTC 1 cut(s) 295
EarI CTCTTC 1 cut(s) 295
Eco105I TACGTA 1 cut(s) 315
Eco47I GGWCC 2 cut(s) 37, 563
Eco57I CTGAAG 1 cut(s) 714
EcoRI GAATTC 2 cut(s) 340, 625
EcoRII CCWGG 1 cut(s) 656
FaeI CATG 5 cut(s) 61, 425, 445, 494, 668
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 2 cut(s) 23, 178
FatI CATG 5 cut(s) 57, 421, 441, 490, 664
Fnu4HI GCNGC 2 cut(s) 225, 496
FokI GGATG 1 cut(s) 87
Fsp4HI GCNGC 2 cut(s) 225, 496
FspBI CTAG 4 cut(s) 197, 306, 386, 795
GluI GCNGC 2 cut(s) 225, 496
GsuI CTGGAG 1 cut(s) 193
HaeIII GGCC 2 cut(s) 368, 498
Hin1II CATG 5 cut(s) 61, 425, 445, 494, 668
HincII GTYRAC 1 cut(s) 779
HindII GTYRAC 1 cut(s) 779
HindIII AAGCTT 1 cut(s) 412
HinfI GANTC 3 cut(s) 604, 620, 661
HphI GGTGA 2 cut(s) 332, 777
Hpy166II GTNNAC 1 cut(s) 779
Hpy188I TCNGA 5 cut(s) 465, 477, 573, 609, 811
Hpy188III TCNNGA 2 cut(s) 104, 665
Hpy8I GTNNAC 1 cut(s) 779
HpyAV CCTTC 2 cut(s) 7, 472
HpyCH4III ACNGT 4 cut(s) 115, 404, 452, 776
HpyCH4IV ACGT 3 cut(s) 275, 314, 354
HpyCH4V TGCA 4 cut(s) 138, 166, 335, 640
HpyF3I CTNAG 1 cut(s) 464
HpySE526I ACGT 3 cut(s) 275, 314, 354
Hsp92II CATG 5 cut(s) 61, 425, 445, 494, 668
Kzo9I GATC 2 cut(s) 181, 737
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 4 cut(s) 80, 109, 309, 649
MaeI CTAG 4 cut(s) 197, 306, 386, 795
MaeII ACGT 3 cut(s) 275, 314, 354
MaeIII GTNAC 2 cut(s) 320, 589
MalI GATC 2 cut(s) 183, 739
MboI GATC 2 cut(s) 181, 737
MboII GAAGA 3 cut(s) 22, 282, 707
MluCI AATT 5 cut(s) 229, 340, 541, 567, 625
MlyI GAGTC 1 cut(s) 670
MmeI TCCRAC 1 cut(s) 673
MnlI CCTC 8 cut(s) 92, 179, 256, 443, 471, 560, 802, 805
MseI TTAA 3 cut(s) 29, 348, 823
MslI CAYNNNNRTG 2 cut(s) 440, 747
MspR9I CCNGG 1 cut(s) 658
Mva1269I GAATGC 1 cut(s) 360
MvaI CCWGG 1 cut(s) 658
NdeII GATC 2 cut(s) 181, 737
NlaIII CATG 5 cut(s) 61, 425, 445, 494, 668
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 320, 589
NspI RCATGY 1 cut(s) 445
NspV TTCGAA 1 cut(s) 623
PaeI GCATGC 1 cut(s) 445
PagI TCATGA 1 cut(s) 664
PaqCI CACCTGC 1 cut(s) 796
PcsI WCGNNNNNNNCGW 1 cut(s) 729
PctI GAATGC 1 cut(s) 360
PfeI GAWTC 2 cut(s) 604, 620
PkrI GCNGC 2 cut(s) 226, 497
PleI GAGTC 1 cut(s) 669
PpsI GAGTC 1 cut(s) 669
Ppu21I YACGTR 1 cut(s) 315
PshAI GACNNNNGTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 656
PspGI CCWGG 1 cut(s) 656
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 37, 563
PstI CTGCAG 1 cut(s) 140
RseI CAYNNNNRTG 2 cut(s) 440, 747
SaqAI TTAA 3 cut(s) 29, 348, 823
SatI GCNGC 2 cut(s) 225, 496
Sau3AI GATC 2 cut(s) 181, 737
Sau96I GGNCC 2 cut(s) 37, 563
SchI GAGTC 1 cut(s) 670
ScrFI CCNGG 1 cut(s) 658
SfaNI GCATC 4 cut(s) 80, 109, 309, 649
SfcI CTRYAG 2 cut(s) 85, 136
SfuI TTCGAA 1 cut(s) 623
SinI GGWCC 2 cut(s) 37, 563
SmiMI CAYNNNNRTG 2 cut(s) 440, 747
SmlI CTYRAG 2 cut(s) 256, 762
SmoI CTYRAG 2 cut(s) 256, 762
SnaBI TACGTA 1 cut(s) 315
SpeI ACTAGT 1 cut(s) 305
SphI GCATGC 1 cut(s) 445
Sse9I AATT 5 cut(s) 229, 340, 541, 567, 625
SsiI CCGC 1 cut(s) 495
SspMI CTAG 4 cut(s) 197, 306, 386, 795
StyD4I CCNGG 1 cut(s) 656
TaaI ACNGT 4 cut(s) 115, 404, 452, 776
TaiI ACGT 3 cut(s) 278, 317, 357
TaqI TCGA 3 cut(s) 184, 623, 723
TasI AATT 5 cut(s) 229, 340, 541, 567, 625
TauI GCSGC 1 cut(s) 498
TfiI GAWTC 2 cut(s) 604, 620
Tru1I TTAA 3 cut(s) 29, 348, 823
Tru9I TTAA 3 cut(s) 29, 348, 823
TscAI CASTG 4 cut(s) 140, 407, 457, 594
TseFI GTSAC 2 cut(s) 320, 589
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 2 cut(s) 320, 589
TspDTI ATGAA 2 cut(s) 141, 353
TspRI CASTG 4 cut(s) 140, 407, 457, 594
VpaK11BI GGWCC 2 cut(s) 37, 563
XapI RAATTY 3 cut(s) 229, 340, 625
XceI RCATGY 1 cut(s) 445
XspI CTAG 4 cut(s) 197, 306, 386, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.