pycom06g11910

glutamyl-tRNA(Gln) amidotransferase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004947
Physical Location & Seq
Forward (+)
17099582 .. 17103053
3472 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1803 bp
ATGATGCTCAAGAACAAGTTTCTTCCATCAGTTAGGGGGTGGTGGCCCCACAGTGGCATCATCGCCGTCGTCGTGCTTCTAGCTCTCATCTGTTGCTGCCGCCCTGTAGCTACTTCAGATGTTAAAAATATGGAGAGTGATCACAAGGATGGTGTGTGTTGGACGAGAGTTGGTCAACCTTGCTCCCTCAAATGTGCATTTGAGTTGTTTGACACCAAATTCTTGAATAACACAAAGATGCTGGAGATTGTGAGGGGTCAAAAGGAGTTTAACCTCCCCATAATCACAGCTAATAGAAAATTAGTTGCTTCTGAGGATGGAGGTTTGCGTAACCCCTCTTATTTGGTATTCAATTCTGGATGGGGCAGGGAACAGTCTCAGCATGCCACTAAAAAGTTCAATTATCCTTCTCTTTCCGGCATCAAAAAACCGAGAAGTGAAGAAGATATCGCCTTTATGAGTGTTCTTGAATTAGGAGAGCTCATTAGAACAAAGCAAATTACGTCGAAGGAGCTTACGAGAATTTTTCTGAAAAGACTGAAAAGGTACAATCATGTTCTTGAGGCTGTGGTGACATATACAGAAGAGTTAGCATACAAGCAAGCAAAAGAGGCTGATCAATTGCTTGCCAAAGGAGTGTATTTGGGTCCTCTGCATGGGATTCCTTATGGATTGAAGGATATAATTGCAGTACCGCAATACAAAACAACGTGGGGTTCCAGTAGTTTCAAAAATCAAGTTCTTGACATTGAAGCTTGGGTTTACAAGAGGCTGAAGGCTGCAGGGGCAGTTCTCGTCGCGAAGCTTGTTTCCGGATCACTTGCGTATGATGACATCTGGTTTGGTGGTCGAACAAGGAACCCTTGGAATATCGAGGAGTTCTCCACAGGTTCATCAGCCGGCCCTGCTGCCTGCACTTCAGCAGGTATGGTTCCATTCGCAATTGGATCAGAAACAGCTGGATCTGTGACCTACCCTGCTGCACGCTGTGGCGTGACAGCATTGCGCCCAACGTTTGGCACTACTGGGCGAACTGGTGTAATGAGCATATCGGAAAGCTTGGACAAGCTTGGACCTTTCTGTCGAAGCGCCGCAGACTGTGCCATTGTTCTGGATGCCATTAGAGGAAAGGACCCAGATGATCTTTCGTCAAGAGACATTCCTCTTGGTGATCCGTTTTCGGTTGACATTAAAAAACTTACAGTTGGATATTTAGAAGATGCTGAGATGGAGGTTGTTCATATTCTTGCATCAGAAGGTGTAAAAATGATCCCTTTCAAGCTTAACTACACAGTTGATTCTGTTCAAGGCATCCTAAATTTTACCATGGACGTCGACATGTTGGCTCACTTTGATGAATGGCAGCGCTCCCGCCATGATGATGTTTACGAAGCTCAAGAACAGTGGCCTACTGAACTCCGCCGTGCACGTGTAATTCCTGCAGTTGATTATGTACAAGCTCAAAGAGCAAGGGGGAAGTTAATCCGCGAAGTAAAAGAAAGTTTTACCGTGGATGCGTTCATTGGCAATGCAACTGACTGGGAGAGAGTTTGTTTGGGAAATCTTGTTGGTATACCTGTAATTGTTGTTCCAACAGGGTTTAAAAGCATATCAAATCCACCTCATAGCAACCATAGTCGAAGAAGAACCACGATCACCACCGGCATTTATGCTCCTCCTGAGCGTGATCATATTGCTCTAGCACTAGCTATGGCTTTCCAATCCGTCACCAATCACCACAAGCAACGGCCTCCGATCGATAATCTTGGACCAAGTGATCCAATCCAAAGTCTTCCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amidase

Protein Analysis

601

Amino Acids

66.52

Weight (kDa)

8.68

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 171 - 415 1e-57 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1080
AatII GACGTC 1 cut(s) 1335
Acc36I ACCTGC 1 cut(s) 914
AccB7I CCANNNNNTGG 1 cut(s) 1016
AccI GTMKAC 2 cut(s) 1335, 1573
AccII CGCG 2 cut(s) 800, 1488
AccIII TCCGGA 1 cut(s) 812
AciI CCGC 6 cut(s) 100, 695, 1092, 1372, 1420, 1486
AclI AACGTT 1 cut(s) 1013
AclWI GGATC 6 cut(s) 823, 955, 970, 1166, 1264, 1772
AcsI RAATTY 3 cut(s) 218, 522, 1318
AcuI CTGAAG 3 cut(s) 99, 794, 903
AcvI CACGTG 1 cut(s) 1430
AcyI GRCGYC 1 cut(s) 1332
AdeI CACNNNGTG 1 cut(s) 989
AfaI GTAC 3 cut(s) 548, 693, 1455
AfeI AGCGCT 1 cut(s) 1367
AfiI CCNNNNNNNGG 3 cut(s) 53, 656, 1016
AflIII ACRYGT 2 cut(s) 1338, 1429
AgsI TTSAA 9 cut(s) 226, 352, 400, 470, 676, 730, 752, 1279, 1307
Alw21I GWGCWC 2 cut(s) 483, 1429
Alw26I GTCTC 2 cut(s) 381, 1149
Alw44I GTGCAC 1 cut(s) 1425
AlwI GGATC 6 cut(s) 823, 955, 970, 1166, 1264, 1772
Aor13HI TCCGGA 1 cut(s) 812
Aor51HI AGCGCT 1 cut(s) 1367
AoxI GGCC 4 cut(s) 44, 901, 1406, 1748
ApaLI GTGCAC 1 cut(s) 1425
ApeKI GCWGC 5 cut(s) 96, 779, 908, 980, 1363
ApoI RAATTY 3 cut(s) 218, 522, 1318
ArsI GACNNNNNNTTYG 2 cut(s) 824, 856
AspLEI GCGC 3 cut(s) 1008, 1091, 1368
AspS9I GGNCC 6 cut(s) 45, 647, 902, 1073, 1132, 1769
AsuHPI GGTGA 5 cut(s) 583, 1181, 1648, 1720, 1727
AvaII GGWCC 4 cut(s) 647, 1073, 1132, 1769
BaeGI GKGCMC 1 cut(s) 1429
BanII GRGCYC 1 cut(s) 483
BbrPI CACGTG 1 cut(s) 1430
BbsI GAAGAC 1 cut(s) 1784
Bbv12I GWGCWC 2 cut(s) 483, 1429
BbvI GCAGC 5 cut(s) 83, 766, 895, 967, 1375
BccI CCATC 5 cut(s) 34, 143, 311, 354, 1222
BceAI ACGGC 3 cut(s) 50, 1407, 1763
BclI TGATCA 3 cut(s) 139, 616, 1687
BcoDI GTCTC 2 cut(s) 381, 1149
BfaI CTAG 3 cut(s) 80, 1700, 1706
BfmI CTRYAG 3 cut(s) 105, 780, 1440
BfoI RGCGCY 2 cut(s) 1092, 1369
BfuAI ACCTGC 1 cut(s) 914
BisI GCNGC 7 cut(s) 97, 100, 780, 909, 981, 1092, 1364
BlsI GCNGC 7 cut(s) 98, 101, 781, 910, 982, 1093, 1365
Bme18I GGWCC 4 cut(s) 647, 1073, 1132, 1769
BmgT120I GGNCC 6 cut(s) 45, 647, 902, 1073, 1132, 1769
BmiI GGNNCC 6 cut(s) 47, 648, 718, 860, 933, 1134
BmrI ACTGGG 2 cut(s) 1035, 1549
BmsI GCATC 8 cut(s) 66, 228, 429, 1105, 1210, 1259, 1320, 1504
BmuI ACTGGG 2 cut(s) 1035, 1549
BpiI GAAGAC 1 cut(s) 1784
BplI GAGNNNNNCTC 2 cut(s) 866, 898
BpmI CTGGAG 1 cut(s) 263
Bpu10I CCTNAGC 1 cut(s) 1680
BpuEI CTTGAG 2 cut(s) 581, 1380
Bsa29I ATCGAT 1 cut(s) 1758
BsaAI YACGTR 1 cut(s) 1430
BsaHI GRCGYC 1 cut(s) 1332
BsaJI CCNNGG 3 cut(s) 863, 1326, 1509
BsaWI WCCGGW 1 cut(s) 812
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 656, 1016
Bse118I RCCGGY 2 cut(s) 899, 1661
Bse1I ACTGG 4 cut(s) 720, 1030, 1039, 1544
Bse3DI GCAATG 2 cut(s) 1001, 1534
BseAI TCCGGA 1 cut(s) 812
BseCI ATCGAT 1 cut(s) 1758
BseDI CCNNGG 3 cut(s) 863, 1326, 1509
BseGI GGATG 6 cut(s) 154, 322, 365, 1120, 1311, 1519
BseLI CCNNNNNNNGG 3 cut(s) 53, 656, 1016
BseMI GCAATG 2 cut(s) 1001, 1534
BseMII CTCAG 4 cut(s) 303, 392, 1215, 1671
BseNI ACTGG 4 cut(s) 720, 1030, 1039, 1544
BseRI GAGGAG 2 cut(s) 890, 1665
BseSI GKGCMC 1 cut(s) 1429
BseXI GCAGC 5 cut(s) 83, 766, 895, 967, 1375
BsgI GTGCAG 2 cut(s) 898, 966
Bsh1236I CGCG 2 cut(s) 800, 1488
Bsh1285I CGRYCG 1 cut(s) 1758
BshFI GGCC 4 cut(s) 46, 903, 1408, 1750
BshVI ATCGAT 1 cut(s) 1758
BsiEI CGRYCG 1 cut(s) 1758
BsiHKAI GWGCWC 2 cut(s) 483, 1429
BsiSI CCGG 4 cut(s) 417, 813, 900, 1662
BslI CCNNNNNNNGG 3 cut(s) 53, 656, 1016
BsmAI GTCTC 2 cut(s) 381, 1149
BsnI GGCC 4 cut(s) 46, 903, 1408, 1750
Bsp1286I GDGCHC 2 cut(s) 483, 1429
Bsp13I TCCGGA 1 cut(s) 812
Bsp1407I TGTACA 1 cut(s) 1453
Bsp19I CCATGG 1 cut(s) 1326
Bsp68I TCGCGA 1 cut(s) 800
BspACI CCGC 6 cut(s) 100, 695, 1092, 1372, 1420, 1486
BspANI GGCC 4 cut(s) 46, 903, 1408, 1750
BspCNI CTCAG 4 cut(s) 304, 391, 1216, 1672
BspDI ATCGAT 1 cut(s) 1758
BspEI TCCGGA 1 cut(s) 812
BspFNI CGCG 2 cut(s) 800, 1488
BspLI GGNNCC 6 cut(s) 47, 648, 718, 860, 933, 1134
BspMAI CTGCAG 2 cut(s) 784, 1444
BspMI ACCTGC 1 cut(s) 914
BspPI GGATC 6 cut(s) 823, 955, 970, 1166, 1264, 1772
BsrDI GCAATG 2 cut(s) 1001, 1534
BsrFI RCCGGY 2 cut(s) 899, 1661
BsrGI TGTACA 1 cut(s) 1453
BsrI ACTGG 4 cut(s) 720, 1030, 1039, 1544
BssAI RCCGGY 2 cut(s) 899, 1661
BssECI CCNNGG 3 cut(s) 863, 1326, 1509
BssNAI GTATAC 1 cut(s) 1574
BssNI GRCGYC 1 cut(s) 1332
BssT1I CCWWGG 2 cut(s) 863, 1326
Bst1107I GTATAC 1 cut(s) 1574
Bst4CI ACNGT 7 cut(s) 53, 375, 1100, 1204, 1294, 1404, 1510
Bst6I CTCTTC 1 cut(s) 579
BstACI GRCGYC 1 cut(s) 1332
BstAPI GCANNNNNTGC 1 cut(s) 1100
BstAUI TGTACA 1 cut(s) 1453
BstBAI YACGTR 1 cut(s) 1430
BstC8I GCNNGC 6 cut(s) 384, 603, 627, 901, 913, 985
BstDEI CTNAG 4 cut(s) 312, 378, 1224, 1680
BstDSI CCRYGG 2 cut(s) 1326, 1509
BstF5I GGATG 6 cut(s) 154, 322, 365, 1120, 1311, 1519
BstFNI CGCG 2 cut(s) 800, 1488
BstH2I RGCGCY 2 cut(s) 1092, 1369
BstHHI GCGC 3 cut(s) 1008, 1091, 1368
BstMAI GTCTC 2 cut(s) 381, 1149
BstMCI CGRYCG 1 cut(s) 1758
BstMWI GCNNNNNNNGC 5 cut(s) 611, 785, 905, 1100, 1466
BstNSI RCATGY 2 cut(s) 386, 1342
BstSFI CTRYAG 3 cut(s) 105, 780, 1440
BstSLI GKGCMC 1 cut(s) 1429
BstUI CGCG 2 cut(s) 800, 1488
BstV1I GCAGC 5 cut(s) 83, 766, 895, 967, 1375
BstV2I GAAGAC 1 cut(s) 1784
BstX2I RGATCY 1 cut(s) 962
BstXI CCANNNNNNTGG 1 cut(s) 1111
BstYI RGATCY 1 cut(s) 962
BstZ17I GTATAC 1 cut(s) 1574
Bsu15I ATCGAT 1 cut(s) 1758
BsuRI GGCC 4 cut(s) 46, 903, 1408, 1750
BsuTUI ATCGAT 1 cut(s) 1758
BtgI CCRYGG 2 cut(s) 1326, 1509
BtgZI GCGATG 1 cut(s) 46
BtsCI GGATG 6 cut(s) 154, 322, 365, 1120, 1311, 1519
BtsIMutI CAGTG 2 cut(s) 58, 1409
BtuMI TCGCGA 1 cut(s) 800
BveI ACCTGC 1 cut(s) 914
Cac8I GCNNGC 6 cut(s) 384, 603, 627, 901, 913, 985
CfoI GCGC 3 cut(s) 1008, 1091, 1368
Cfr10I RCCGGY 2 cut(s) 899, 1661
Cfr13I GGNCC 6 cut(s) 45, 647, 902, 1073, 1132, 1769
ClaI ATCGAT 1 cut(s) 1758
Csp6I GTAC 3 cut(s) 547, 692, 1454
CspCI CAANNNNNGTGG 2 cut(s) 1385, 1420
CviAII CATG 6 cut(s) 383, 554, 656, 1327, 1339, 1376
CviQI GTAC 3 cut(s) 547, 692, 1454
DdeI CTNAG 4 cut(s) 312, 378, 1224, 1680
DraI TTTAAA 1 cut(s) 1603
DraIII CACNNNGTG 1 cut(s) 989
DrdI GACNNNNNNGTC 1 cut(s) 1080
DseDI GACNNNNNNGTC 1 cut(s) 1080
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
EciI GGCGGA 1 cut(s) 1409
Ecl136II GAGCTC 1 cut(s) 481
Eco130I CCWWGG 2 cut(s) 863, 1326
Eco24I GRGCYC 1 cut(s) 483
Eco32I GATATC 1 cut(s) 448
Eco47I GGWCC 4 cut(s) 647, 1073, 1132, 1769
Eco47III AGCGCT 1 cut(s) 1367
Eco53kI GAGCTC 1 cut(s) 481
Eco57I CTGAAG 3 cut(s) 99, 794, 903
Eco72I CACGTG 1 cut(s) 1430
EcoICRI GAGCTC 1 cut(s) 481
EcoO109I RGGNCCY 2 cut(s) 647, 1132
EcoRV GATATC 1 cut(s) 448
EcoT14I CCWWGG 2 cut(s) 863, 1326
EcoT38I GRGCYC 1 cut(s) 483
ErhI CCWWGG 2 cut(s) 863, 1326
FaeI CATG 6 cut(s) 386, 557, 659, 1330, 1342, 1379
FalI AAGNNNNNCTT 2 cut(s) 847, 879
FatI CATG 6 cut(s) 382, 553, 655, 1326, 1338, 1375
FauI CCCGC 1 cut(s) 1379
FbaI TGATCA 3 cut(s) 139, 616, 1687
FblI GTMKAC 2 cut(s) 1335, 1573
Fnu4HI GCNGC 7 cut(s) 97, 100, 780, 909, 981, 1092, 1364
FokI GGATG 6 cut(s) 161, 329, 372, 1127, 1298, 1526
FriOI GRGCYC 1 cut(s) 483
Fsp4HI GCNGC 7 cut(s) 97, 100, 780, 909, 981, 1092, 1364
FspBI CTAG 3 cut(s) 80, 1700, 1706
GlaI GCGC 3 cut(s) 1007, 1090, 1367
GluI GCNGC 7 cut(s) 97, 100, 780, 909, 981, 1092, 1364
GsuI CTGGAG 1 cut(s) 263
HaeII RGCGCY 2 cut(s) 1092, 1369
HaeIII GGCC 4 cut(s) 46, 903, 1408, 1750
HapII CCGG 4 cut(s) 417, 813, 900, 1662
HhaI GCGC 3 cut(s) 1008, 1091, 1368
Hin1I GRCGYC 1 cut(s) 1332
Hin1II CATG 6 cut(s) 386, 557, 659, 1330, 1342, 1379
Hin6I GCGC 3 cut(s) 1006, 1089, 1366
HinP1I GCGC 3 cut(s) 1006, 1089, 1366
HincII GTYRAC 3 cut(s) 176, 1186, 1336
HindII GTYRAC 3 cut(s) 176, 1186, 1336
HindIII AAGCTT 5 cut(s) 753, 803, 1057, 1067, 1280
HinfI GANTC 2 cut(s) 661, 1298
HpaII CCGG 4 cut(s) 417, 813, 900, 1662
HphI GGTGA 5 cut(s) 583, 1181, 1648, 1720, 1727
Hpy166II GTNNAC 7 cut(s) 176, 763, 1186, 1336, 1387, 1427, 1574
Hpy188I TCNGA 7 cut(s) 118, 313, 531, 952, 1054, 1255, 1755
Hpy8I GTNNAC 7 cut(s) 176, 763, 1186, 1336, 1387, 1427, 1574
Hpy99I CGWCG 5 cut(s) 71, 74, 508, 800, 1337
HpyAV CCTTC 5 cut(s) 417, 502, 670, 769, 1250
HpyCH4III ACNGT 7 cut(s) 53, 375, 1100, 1204, 1294, 1404, 1510
HpyCH4IV ACGT 5 cut(s) 503, 710, 1013, 1332, 1429
HpyF10VI GCNNNNNNNGC 5 cut(s) 611, 785, 905, 1100, 1466
HpyF3I CTNAG 4 cut(s) 312, 378, 1224, 1680
HpySE526I ACGT 5 cut(s) 503, 710, 1013, 1332, 1429
Hsp92I GRCGYC 1 cut(s) 1332
Hsp92II CATG 6 cut(s) 386, 557, 659, 1330, 1342, 1379
HspAI GCGC 3 cut(s) 1006, 1089, 1366
Kpn2I TCCGGA 1 cut(s) 812
KroI GCCGGC 1 cut(s) 899
KroNI GCCGGC 1 cut(s) 901
Ksp22I TGATCA 3 cut(s) 139, 616, 1687
LmnI GCTCC 4 cut(s) 188, 511, 1373, 1678
Lsp1109I GCAGC 5 cut(s) 83, 766, 895, 967, 1375
LweI GCATC 8 cut(s) 66, 228, 429, 1105, 1210, 1259, 1320, 1504
MaeI CTAG 3 cut(s) 80, 1700, 1706
MaeII ACGT 5 cut(s) 503, 710, 1013, 1332, 1429
MaeIII GTNAC 5 cut(s) 329, 571, 967, 994, 1726
MboII GAAGA 8 cut(s) 14, 452, 455, 596, 1229, 1653, 1656, 1784
MfeI CAATTG 2 cut(s) 620, 942
MflI RGATCY 1 cut(s) 962
MhlI GDGCHC 2 cut(s) 483, 1429
MmeI TCCRAC 3 cut(s) 140, 1186, 1616
MroI TCCGGA 1 cut(s) 812
MroNI GCCGGC 1 cut(s) 899
MseI TTAA 6 cut(s) 123, 270, 1191, 1284, 1481, 1602
MslI CAYNNNNRTG 4 cut(s) 147, 236, 1353, 1380
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 4 cut(s) 417, 813, 900, 1662
MunI CAATTG 2 cut(s) 620, 942
MvnI CGCG 2 cut(s) 800, 1488
MwoI GCNNNNNNNGC 5 cut(s) 611, 785, 905, 1100, 1466
NaeI GCCGGC 1 cut(s) 901
NcoI CCATGG 1 cut(s) 1326
NgoMIV GCCGGC 1 cut(s) 899
NlaIII CATG 6 cut(s) 386, 557, 659, 1330, 1342, 1379
NlaIV GGNNCC 6 cut(s) 47, 648, 718, 860, 933, 1134
NmuCI GTSAC 4 cut(s) 571, 967, 994, 1726
NruI TCGCGA 1 cut(s) 800
NspI RCATGY 2 cut(s) 386, 1342
PaeI GCATGC 1 cut(s) 386
PciI ACATGT 1 cut(s) 1338
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PdiI GCCGGC 1 cut(s) 901
PfeI GAWTC 2 cut(s) 661, 1298
PflMI CCANNNNNTGG 1 cut(s) 1016
PkrI GCNGC 7 cut(s) 98, 101, 781, 910, 982, 1093, 1365
Ple19I CGATCG 1 cut(s) 1758
PmaCI CACGTG 1 cut(s) 1430
PmlI CACGTG 1 cut(s) 1430
Ppu21I YACGTR 1 cut(s) 1430
PpuMI RGGWCCY 2 cut(s) 647, 1132
PscI ACATGT 1 cut(s) 1338
Psp124BI GAGCTC 1 cut(s) 483
Psp1406I AACGTT 1 cut(s) 1013
Psp5II RGGWCCY 2 cut(s) 647, 1132
PspCI CACGTG 1 cut(s) 1430
PspN4I GGNNCC 6 cut(s) 47, 648, 718, 860, 933, 1134
PspPI GGNCC 6 cut(s) 45, 647, 902, 1073, 1132, 1769
PspPPI RGGWCCY 2 cut(s) 647, 1132
PsrI GAACNNNNNNTAC 2 cut(s) 1572, 1604
PstI CTGCAG 2 cut(s) 784, 1444
PsuI RGATCY 1 cut(s) 962
PvuI CGATCG 1 cut(s) 1758
PvuII CAGCTG 1 cut(s) 959
RruI TCGCGA 1 cut(s) 800
RsaI GTAC 3 cut(s) 548, 693, 1455
RsaNI GTAC 3 cut(s) 547, 692, 1454
RseI CAYNNNNRTG 4 cut(s) 147, 236, 1353, 1380
SacI GAGCTC 1 cut(s) 483
SalI GTCGAC 1 cut(s) 1334
SaqAI TTAA 6 cut(s) 123, 270, 1191, 1284, 1481, 1602
SatI GCNGC 7 cut(s) 97, 100, 780, 909, 981, 1092, 1364
Sau96I GGNCC 6 cut(s) 45, 647, 902, 1073, 1132, 1769
SduI GDGCHC 2 cut(s) 483, 1429
SfaNI GCATC 8 cut(s) 66, 228, 429, 1105, 1210, 1259, 1320, 1504
SfcI CTRYAG 3 cut(s) 105, 780, 1440
SinI GGWCC 4 cut(s) 647, 1073, 1132, 1769
SmiMI CAYNNNNRTG 4 cut(s) 147, 236, 1353, 1380
SmlI CTYRAG 3 cut(s) 8, 560, 1395
SmoI CTYRAG 3 cut(s) 8, 560, 1395
SphI GCATGC 1 cut(s) 386
SsiI CCGC 6 cut(s) 100, 695, 1092, 1372, 1420, 1486
SspMI CTAG 3 cut(s) 80, 1700, 1706
SstI GAGCTC 1 cut(s) 483
StyI CCWWGG 2 cut(s) 863, 1326
TaaI ACNGT 7 cut(s) 53, 375, 1100, 1204, 1294, 1404, 1510
TaiI ACGT 5 cut(s) 506, 713, 1016, 1335, 1432
TaqI TCGA 7 cut(s) 506, 850, 873, 1084, 1335, 1639, 1758
TatI WGTACW 1 cut(s) 1453
TauI GCSGC 2 cut(s) 102, 1094
TfiI GAWTC 2 cut(s) 661, 1298
Tru1I TTAA 6 cut(s) 123, 270, 1191, 1284, 1481, 1602
Tru9I TTAA 6 cut(s) 123, 270, 1191, 1284, 1481, 1602
TscAI CASTG 2 cut(s) 58, 1409
TseFI GTSAC 4 cut(s) 571, 967, 994, 1726
TseI GCWGC 5 cut(s) 96, 779, 908, 980, 1363
Tsp45I GTSAC 4 cut(s) 571, 967, 994, 1726
TspDTI ATGAA 4 cut(s) 882, 1229, 1371, 1510
TspGWI ACGGA 2 cut(s) 1164, 1714
TspRI CASTG 2 cut(s) 58, 1409
Van91I CCANNNNNTGG 1 cut(s) 1016
VneI GTGCAC 1 cut(s) 1425
VpaK11BI GGWCC 4 cut(s) 647, 1073, 1132, 1769
XapI RAATTY 3 cut(s) 218, 522, 1318
XceI RCATGY 2 cut(s) 386, 1342
XmiI GTMKAC 2 cut(s) 1335, 1573
XspI CTAG 3 cut(s) 80, 1700, 1706
ZraI GACGTC 1 cut(s) 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.