pycom06g15160

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013666
Physical Location & Seq
Forward (+)
19759347 .. 19763659
4313 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3357 bp
ATGCCTGTTCATCCATGGACGACCCTCCTCTCCTCCTCCTTATTCATGTTGCTCCTCCTCTCTTTTTCTCTCGCCGCCTCAGCCTTGAACCCGCAAGGCCGAGCTTTGCTCTCATGGAAGCAGAGCCTCAACGGCTCTGTTTCCACGAGTGCATTGGGAAATTGGGACCCGAATGACGAAGCGCCGTGCGGGTGGTTTGGGGTCACCTGCAACTCCAACGGCCAAGTTGTGGAGTTGAATTTGAAGTACCTTGATTTGCTGGGTCAGCTCCCTTGTAACTTCACCTCGTTGTCGACCATCACCAAGCTCACGCTCTCCGGCACCAACCTCACCGGCTCGATCCCGAAACAGATTTCCACTCTCAGAGACCTCACCGTACTGGACTTGAGCGACAATGCATTAACCGGCGAAATCCCAACCGAGATCTGCTCGCTGCCGAAGCTCGAGCAGCTTCACCTCAGCGCGAATCGATTGGAAGGCGAAATCCCAACCCAAATCGGCAACCTCACCAGCTTGAAATGGCTGGTTCTTTACGACAACCAGCTCAGCGGCAACATTCCGAAATCGATTGGCGATTTGCACAATCTTGAAGTGATCAGAGCCGGCGGGAACAAGAACCTCGAAGGCCCTGTGCCCCACGAGATTGGAAGCTGCAACAATTTGGTAATGCTGGGATTGGCAGAAACCAGCATTTCGGGCTTCCTCCCTCCATCTCTTGGCCTCCTCAAGAAACTCGAAACACTCGCAATCTACACCGCCTTGCTCTCAGGTCCGATCCCACCGGAGCTTGGAGACTGCTCCGAGCTCCGGGACATCTATTTGTATGAGAATTCGATAACGGGATCGGTCCCAAGCCAATTGGGGAAGTTAAACAACCTTCAGAACCTTCTTCTCTGGCAGAACAACTTGGTGGGAGTCATTCCGCCGGAGCTCGGCAACTGCCTGCAGCTATTAGTCATCGACATCTCGATGAACTCATTGACTGGAATCATTCCCCTGTCTTTCGGAAACTTGACTTCGCTTCAGGAGCTGCAGCTGAGCGTCAATCAAATATCGGGCGAGATTCCGGCGCAGCTCGGAAACTGCCGGAAACTGACGCAGATCGAGCTCGACAATAACCAGATCACCGGTTCGATACCGGCGGAGTTCGGAAACTTGTCGAACCTTTCTCTTCTTTTCTTGTGGCAGAACAAGCTCGAAGGAAGCGTTCCGCCGTCGATTTCCAACTGTGTTAACCTCGAGTCCGTTGATTTGTCACAGAACGGCTTAACGGGGCCGATTCCCGGAGGGCTCTTCAATCTCCAGAATCTCACCAAGCTGCTGCTGCTATCTAACAACTTCTCCGGTGAAATTCCGTCGGAGATCGGAAACTGCTCGTCGCTGATTCGGTTTCGGGCCAGCGGCAACAAACTCGCGGGAGGGATTCCGCCGCAGATTGGGAATTTGAGAAACTTGAATTTCTTGGATCTTGGATCGAATCGGCTTAGTCGAACCATACCGGAGGAGATCTCCGGCTGCCGGAATCTGACGTTTCTGGATTTGCATTCCAATTCGATTGGGGGAGATTTGCCTGGAGGTTTTGATAAGCTTGTATATCTCCAATTCGTGGATTTCTCAGACAATTTGATCGAAGGGACGTTGAGACCTGCAATCGGATCACTGAGTTCACTCACCAAGCTCGTTTTGGGAAATAATCGGTTCGCGGGTGCGATTCCGAGTGAACTCGGTTCGTGCTCGAAGCTACAGTTGTTGGATTTGAGCGGGAACGAGCTGACAGGGAATATTCCGGCCAGCTTGGGCAAGATTCCCGCGCTGGAAATCGCTCTGAACCTGAGCTTGAACCAACTCTCCGGCGAGATACCGAAGGAGTTCGCCGATTTGGACAAGCTCGGGATTCTAGACGTCTCTCACAATCAGCTCTCCGGCGACCTCCAATTTCTTGCGGACATGCAAAATCTCGTGGTCCTCAATATCTCGCACAACAACTTCTCAGGGAGAGTCCCAGACACGCCCTTCTTTTCGAAGCTTCCACTGAGCGTCTTGTCCGGCAATCCCTCGCTCTGTCTCTCCGGAAACAGCCAGTGCTCCGCCGCGAACAAAAATCCCGACGGATCCCGCCGCCGCAATGTAGCAGCGCGCGTGGCCATGGTGGTGCTACTGTGCACCGCGTGCACGCTTCTTCTCACGGCGTTCTACATTATCCTCGGAGCAAAGATGCGGGGCCCGGCCGGACTCTTCGGAGGGTCCCATGAGCCCGACCCGGAGGACGACAGCGAGTTAGACGTGGGCCCACCGTGGGAGGTTACGCTTTACCAGAAGCTCGACCTGTCGATTGTCGACGTGGCGCGAAGTTTAACTCCTTGCAACGTCATCGGACGGGGCCGCTCGGGCGTCGTTTATCAAGTCACCATCCCCTCAGGCTTATCCATCGCAGTAAAAAGATTCCGTCCGTCAGAGAAATACTCGGCCTCCGCATTTTCCTCGGAGATTGCCACGTTGGCAAGAATACGACACCGTAACATCGTACGGTTACTCGGTTGGGGCGCGAACCGGAGGACGAAGTTACTGTTTTACGACCATCTGGTCAACGGTAATCTGGGTTCGTTGCTGCACGAGGGGTGCGCAGGATTAGTGGAGTGGGACACCCGGTTCAGGATAGCGTCGGGCGTGGCGGAGGGGTTGGCGTACTTGCACCACGATTGCCAGCCGGCTATCTTACACCGCGACGTGAAGGCGCAAAACATTCTGCTGGGGGATCGATACGAGGCCGTTTTGGCGGACTTTGGACTTGCTAGGCTGGTGGAGGACGATCCAAACGGTCCATTTTCAGCCAACCCCCAATTTGCCGGGTCCTACGGCTACATTGCGCCGGAATATGCCTGCATGCTAAAAATCACTGCAAAAAGCGATGTGTATAGCTATGGGGTTGTCCTTTTGGAGATCATAACTGGGAAAAGGCCAGTGGACCCATCATTCACAGATGGGCAACATGTGATTCAGTGGGTCCGAGACCACCTGAAGAGCAAGAAGGACCCTGTTGAGATTCTTGATCCTAAACTTCAAGGTTATCCAGACACACAGATACAAGAAATGCTCCAAGCTCTTGGAATCTCACTCCTCTGCACCAGCAACCGGGCGGAGGATCGTCCCACCATGAAGGACGTGGCGGCATTGTTGAGAGAAATTCGACACGATCAGGGCCCGGTGGCAAGCGAGGTGCATAAGCCGGCTACCAACGCCTTGAAGAATTCATCATCTTCTGTGACACCGGCTCAGTTGCTTCAGCTGCAAGGCTCCTCTCATTGCTCACTTGCTTACTCATCTGCTTCAGCTGGTTATCTGTCAGGAACTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1119

Amino Acids

120.33

Weight (kDa)

5.5

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 71 1.3e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 99 - 158 4.4e-09 Leucine rich repeat
LRR_14 PF23598 100 - 200 1.7e-12 Leucine-rich repeat region
LRR_14 PF23598 263 - 371 6.8e-08 Leucine-rich repeat region
LRR_8 PF13855 292 - 351 2.9e-06 Leucine rich repeat
LRR_8 PF13855 363 - 423 3.7e-06 Leucine rich repeat
LRR_14 PF23598 475 - 665 1e-09 Leucine-rich repeat region
Pkinase PF00069 789 - 1055 7.5e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 789 - 1056 4.8e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013666)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05780 FvH4_5g05780 FvH4_5g05780
malus_domestica MD06G1170200.v1.1
prunus_persica Prupe.5G169700_v2.0.a1
pyrus_communis pycom06g15160
rosa_chinensis RchiOBHm_Chr7g0193041
rosa_laevigata RLG00000004301
rosa_multiflora Rmu_co8431803.1_g000001 Rmu_co8478551.1_g000001 Rmu_sc0002040.1_g000078
rosa_roxburghii Rroxscaffold_3G00262440
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132300 Rh7BG133200 Rh7CG136300 Rh7DG134200
rosa_wichuraiana Rw7G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 215
AasI GACNNNNNNGTC 1 cut(s) 2582
AatII GACGTC 1 cut(s) 1905
Acc16I TGCGCA 1 cut(s) 2623
Acc36I ACCTGC 2 cut(s) 215, 1654
AccB1I GGYRCC 1 cut(s) 320
AccB7I CCANNNNNTGG 3 cut(s) 229, 716, 1606
AccBSI CCGCTC 2 cut(s) 1761, 2385
AccI GTMKAC 2 cut(s) 293, 2337
AccIII TCCGGA 1 cut(s) 2069
AcoI YGGCCR 4 cut(s) 220, 1788, 2142, 2226
AcsI RAATTY 7 cut(s) 238, 829, 1350, 1441, 1456, 3183, 3247
AcuI CTGAAG 5 cut(s) 863, 1007, 3038, 3266, 3312
AcyI GRCGYC 2 cut(s) 1902, 2391
AdeI CACNNNGTG 1 cut(s) 2169
AfaI GTAC 4 cut(s) 248, 378, 2526, 2687
AflIII ACRYGT 1 cut(s) 2989
AgeI ACCGGT 1 cut(s) 1127
AjiI CACGTC 4 cut(s) 2284, 2341, 2728, 3163
AjnI CCWGG 1 cut(s) 1570
AloI GAACNNNNNNTCC 4 cut(s) 1832, 1864, 2633, 2665
Alw21I GWGCWC 7 cut(s) 807, 933, 1110, 1736, 2087, 2165, 2174
Alw26I GTCTC 6 cut(s) 360, 786, 1636, 1909, 2069, 3003
Alw44I GTGCAC 2 cut(s) 2161, 2170
AlwNI CAGNNNCTG 1 cut(s) 1030
Ama87I CYCGRG 4 cut(s) 443, 1238, 1889, 2386
Aor13HI TCCGGA 1 cut(s) 2069
ApaI GGGCCC 3 cut(s) 2225, 2291, 3203
ApaLI GTGCAC 2 cut(s) 2161, 2170
ApoI RAATTY 7 cut(s) 238, 829, 1350, 1441, 1456, 3183, 3247
AsiGI ACCGGT 1 cut(s) 1127
Asp700I GAANNNNTTC 1 cut(s) 1868
AsuC2I CCSGG 8 cut(s) 809, 1284, 2225, 2261, 2647, 2848, 3134, 3203
AsuII TTCGAA 1 cut(s) 2021
AvaI CYCGRG 4 cut(s) 443, 1238, 1889, 2386
AxyI CCTNAGG 1 cut(s) 2416
BaeGI GKGCMC 6 cut(s) 636, 2165, 2174, 2225, 2291, 3203
BalI TGGCCA 1 cut(s) 2144
BamHI GGATCC 1 cut(s) 2111
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 8 cut(s) 807, 933, 1110, 1293, 2225, 2256, 2291, 3203
BauI CACGAG 4 cut(s) 145, 638, 1958, 2612
Bbv12I GWGCWC 7 cut(s) 807, 933, 1110, 1736, 2087, 2165, 2174
BbvCI CCTCAGC 2 cut(s) 79, 458
BccI CCATC 7 cut(s) 305, 718, 2417, 2435, 2586, 2975, 2977
BceAI ACGGC 8 cut(s) 148, 169, 235, 1198, 1279, 2202, 2753, 2872
BcgI CGANNNNNNTGC 6 cut(s) 1393, 1427, 2353, 2387, 2463, 2497
BciT130I CCWGG 1 cut(s) 1572
BclI TGATCA 1 cut(s) 594
BcnI CCSGG 8 cut(s) 809, 1284, 2225, 2261, 2647, 2848, 3134, 3203
BcoDI GTCTC 6 cut(s) 360, 786, 1636, 1909, 2069, 3003
BfaI CTAG 2 cut(s) 1898, 2793
BfmI CTRYAG 3 cut(s) 944, 1031, 1742
BfoI RGCGCY 1 cut(s) 185
BfuAI ACCTGC 2 cut(s) 215, 1654
BglI GCCNNNNNGGC 4 cut(s) 132, 2388, 2498, 2774
BglII AGATCT 2 cut(s) 423, 1506
BlpI GCTNAGC 2 cut(s) 545, 1037
Bme1390I CCNGG 9 cut(s) 809, 1284, 1572, 2225, 2261, 2647, 2848, 3134, 3203
BmeT110I CYCGRG 4 cut(s) 443, 1238, 1889, 2386
BmgBI CACGTC 4 cut(s) 2284, 2341, 2728, 3163
BmrFI CCNGG 9 cut(s) 809, 1284, 1572, 2225, 2261, 2647, 2848, 3134, 3203
BmrI ACTGGG 1 cut(s) 2958
BmsI GCATC 1 cut(s) 2205
BmuI ACTGGG 1 cut(s) 2958
BplI GAGNNNNNCTC 8 cut(s) 93, 125, 413, 445, 1493, 1525, 2447, 2479
BpmI CTGGAG 2 cut(s) 1286, 1593
Bpu10I CCTNAGC 3 cut(s) 79, 458, 1832
Bpu1102I GCTNAGC 2 cut(s) 545, 1037
Bpu14I TTCGAA 1 cut(s) 2021
BpuEI CTTGAG 2 cut(s) 406, 710
BpuMI CCSGG 8 cut(s) 809, 1284, 2225, 2261, 2647, 2848, 3134, 3203
Bsa29I ATCGAT 3 cut(s) 469, 566, 2758
BsaHI GRCGYC 2 cut(s) 1902, 2391
BsaI GGTCTC 3 cut(s) 360, 1636, 3003
BsaJI CCNNGG 5 cut(s) 14, 2145, 2203, 2294, 2481
BsaWI WCCGGW 6 cut(s) 781, 1127, 1343, 1498, 2069, 2550
BsaXI ACNNNNNCTCC 6 cut(s) 1325, 1355, 1832, 1862, 2453, 2483
Bse118I RCCGGY 8 cut(s) 332, 404, 602, 1127, 1138, 2707, 3226, 3268
Bse1I ACTGG 5 cut(s) 384, 988, 2080, 2953, 2960
Bse21I CCTNAGG 1 cut(s) 2416
Bse3DI GCAATG 3 cut(s) 2131, 2862, 3301
BseAI TCCGGA 1 cut(s) 2069
BseBI CCWGG 1 cut(s) 1572
BseCI ATCGAT 3 cut(s) 469, 566, 2758
BseDI CCNNGG 5 cut(s) 14, 2145, 2203, 2294, 2481
BseGI GGATG 2 cut(s) 10, 2409
BseMI GCAATG 3 cut(s) 2131, 2862, 3301
BseNI ACTGG 5 cut(s) 384, 988, 2080, 2953, 2960
BsePI GCGCGC 1 cut(s) 2135
BseRI GAGGAG 9 cut(s) 17, 22, 25, 44, 47, 713, 1517, 3107, 3286
BseSI GKGCMC 6 cut(s) 636, 2165, 2174, 2225, 2291, 3203
BseX3I CGGCCG 1 cut(s) 2226
BseYI CCCAGC 3 cut(s) 259, 670, 2749
BsgI GTGCAG 2 cut(s) 2594, 3106
Bsh1285I CGRYCG 1 cut(s) 2229
BshNI GGYRCC 1 cut(s) 320
BshTI ACCGGT 1 cut(s) 1127
BshVI ATCGAT 3 cut(s) 469, 566, 2758
BsiEI CGRYCG 1 cut(s) 2229
BsiHKAI GWGCWC 7 cut(s) 807, 933, 1110, 1736, 2087, 2165, 2174
BsiHKCI CYCGRG 4 cut(s) 443, 1238, 1889, 2386
BsiWI CGTACG 1 cut(s) 2524
BslFI GGGAC 8 cut(s) 179, 824, 833, 1648, 1985, 2230, 2654, 3132
BsmAI GTCTC 6 cut(s) 360, 786, 1636, 1909, 2069, 3003
BsmBI CGTCTC 1 cut(s) 1909
BsmFI GGGAC 8 cut(s) 179, 824, 833, 1648, 1985, 2230, 2654, 3132
BsmI GAATGC 1 cut(s) 1543
Bso31I GGTCTC 3 cut(s) 360, 1636, 3003
BsoBI CYCGRG 4 cut(s) 443, 1238, 1889, 2386
Bsp119I TTCGAA 1 cut(s) 2021
Bsp120I GGGCCC 3 cut(s) 2221, 2287, 3199
Bsp13I TCCGGA 1 cut(s) 2069
Bsp1720I GCTNAGC 2 cut(s) 545, 1037
Bsp19I CCATGG 2 cut(s) 14, 2145
BspDI ATCGAT 3 cut(s) 469, 566, 2758
BspEI TCCGGA 1 cut(s) 2069
BspMAI CTGCAG 2 cut(s) 948, 1035
BspMI ACCTGC 2 cut(s) 215, 1654
BspQI GCTCTTC 2 cut(s) 1298, 3014
BspT104I TTCGAA 1 cut(s) 2021
BspT107I GGYRCC 1 cut(s) 320
BspTNI GGTCTC 3 cut(s) 360, 1636, 3003
BsrBI CCGCTC 2 cut(s) 1761, 2385
BsrDI GCAATG 3 cut(s) 2131, 2862, 3301
BsrFI RCCGGY 8 cut(s) 332, 404, 602, 1127, 1138, 2707, 3226, 3268
BsrI ACTGG 5 cut(s) 384, 988, 2080, 2953, 2960
BssAI RCCGGY 8 cut(s) 332, 404, 602, 1127, 1138, 2707, 3226, 3268
BssECI CCNNGG 5 cut(s) 14, 2145, 2203, 2294, 2481
BssHII GCGCGC 1 cut(s) 2135
BssNI GRCGYC 2 cut(s) 1902, 2391
BssSI CACGAG 4 cut(s) 145, 638, 1958, 2612
BssT1I CCWWGG 2 cut(s) 14, 2145
Bst2BI CACGAG 4 cut(s) 145, 638, 1958, 2612
Bst2UI CCWGG 1 cut(s) 1572
Bst6I CTCTTC 4 cut(s) 1176, 1298, 2240, 3014
BstACI GRCGYC 2 cut(s) 1902, 2391
BstAPI GCANNNNNTGC 1 cut(s) 2169
BstBI TTCGAA 1 cut(s) 2021
BstDSI CCRYGG 3 cut(s) 14, 2145, 2294
BstEII GGTNACC 1 cut(s) 202
BstF5I GGATG 2 cut(s) 10, 2409
BstH2I RGCGCY 1 cut(s) 185
BstMAI GTCTC 6 cut(s) 360, 786, 1636, 1909, 2069, 3003
BstMCI CGRYCG 1 cut(s) 2229
BstNI CCWGG 1 cut(s) 1572
BstNSI RCATGY 3 cut(s) 1951, 2887, 2993
BstPI GGTNACC 1 cut(s) 202
BstSCI CCNGG 9 cut(s) 807, 1282, 1570, 2223, 2259, 2645, 2846, 3132, 3201
BstSFI CTRYAG 3 cut(s) 944, 1031, 1742
BstSLI GKGCMC 6 cut(s) 636, 2165, 2174, 2225, 2291, 3203
BstX2I RGATCY 4 cut(s) 423, 1465, 1506, 2111
BstXI CCANNNNNNTGG 2 cut(s) 644, 3104
BstYI RGATCY 4 cut(s) 423, 1465, 1506, 2111
BstZI CGGCCG 1 cut(s) 2226
Bsu15I ATCGAT 3 cut(s) 469, 566, 2758
Bsu36I CCTNAGG 1 cut(s) 2416
BsuTUI ATCGAT 3 cut(s) 469, 566, 2758
BtgI CCRYGG 3 cut(s) 14, 2145, 2294
BtgZI GCGATG 2 cut(s) 2413, 2922
BtrI CACGTC 4 cut(s) 2284, 2341, 2728, 3163
BtsCI GGATG 2 cut(s) 10, 2409
BtsI GCAGTG 1 cut(s) 2895
BtsIMutI CAGTG 6 cut(s) 1658, 2030, 2087, 2895, 2967, 3005
BveI ACCTGC 2 cut(s) 215, 1654
CaiI CAGNNNCTG 1 cut(s) 1030
Cfr10I RCCGGY 8 cut(s) 332, 404, 602, 1127, 1138, 2707, 3226, 3268
ClaI ATCGAT 3 cut(s) 469, 566, 2758
CseI GACGC 5 cut(s) 1030, 1105, 2026, 2380, 2649
Csp6I GTAC 4 cut(s) 247, 377, 2525, 2686
CspAI ACCGGT 1 cut(s) 1127
CviAII CATG 9 cut(s) 15, 46, 114, 1948, 2146, 2249, 2884, 2990, 3154
CviQI GTAC 4 cut(s) 247, 377, 2525, 2686
DraIII CACNNNGTG 1 cut(s) 2169
DrdI GACNNNNNNGTC 1 cut(s) 2582
DseDI GACNNNNNNGTC 1 cut(s) 2582
EaeI YGGCCR 4 cut(s) 220, 1788, 2142, 2226
EagI CGGCCG 1 cut(s) 2226
Eam1104I CTCTTC 4 cut(s) 1176, 1298, 2240, 3014
EarI CTCTTC 4 cut(s) 1176, 1298, 2240, 3014
EciI GGCGGA 8 cut(s) 912, 1157, 1200, 1416, 2077, 2687, 2792, 3152
Ecl136II GAGCTC 3 cut(s) 805, 931, 1108
EclXI CGGCCG 1 cut(s) 2226
Eco130I CCWWGG 2 cut(s) 14, 2145
Eco24I GRGCYC 8 cut(s) 807, 933, 1110, 1293, 2225, 2256, 2291, 3203
Eco31I GGTCTC 3 cut(s) 360, 1636, 3003
Eco52I CGGCCG 1 cut(s) 2226
Eco53kI GAGCTC 3 cut(s) 805, 931, 1108
Eco57I CTGAAG 5 cut(s) 863, 1007, 3038, 3266, 3312
Eco81I CCTNAGG 1 cut(s) 2416
Eco88I CYCGRG 4 cut(s) 443, 1238, 1889, 2386
Eco91I GGTNACC 1 cut(s) 202
EcoICRI GAGCTC 3 cut(s) 805, 931, 1108
EcoO109I RGGNCCY 7 cut(s) 166, 626, 2221, 2244, 2850, 3031, 3199
EcoO65I GGTNACC 1 cut(s) 202
EcoRI GAATTC 2 cut(s) 829, 3247
EcoRII CCWGG 1 cut(s) 1570
EcoT14I CCWWGG 2 cut(s) 14, 2145
EcoT22I ATGCAT 1 cut(s) 400
EcoT38I GRGCYC 8 cut(s) 807, 933, 1110, 1293, 2225, 2256, 2291, 3203
ErhI CCWWGG 2 cut(s) 14, 2145
Esp3I CGTCTC 1 cut(s) 1909
FaeI CATG 9 cut(s) 18, 49, 117, 1951, 2149, 2252, 2887, 2993, 3157
FaqI GGGAC 8 cut(s) 179, 824, 833, 1648, 1985, 2230, 2654, 3132
FatI CATG 9 cut(s) 14, 45, 113, 1947, 2145, 2248, 2883, 2989, 3153
FauI CCCGC 9 cut(s) 99, 182, 599, 1408, 1696, 1754, 1816, 2123, 2211
FbaI TGATCA 1 cut(s) 594
FblI GTMKAC 2 cut(s) 293, 2337
FokI GGATG 1 cut(s) 2396
FriOI GRGCYC 8 cut(s) 807, 933, 1110, 1293, 2225, 2256, 2291, 3203
FspBI CTAG 2 cut(s) 1898, 2793
FspI TGCGCA 1 cut(s) 2623
GsaI CCCAGC 3 cut(s) 263, 674, 2753
GsuI CTGGAG 2 cut(s) 1286, 1593
HaeII RGCGCY 1 cut(s) 185
HgaI GACGC 5 cut(s) 1030, 1105, 2026, 2380, 2649
Hin1I GRCGYC 2 cut(s) 1902, 2391
Hin1II CATG 9 cut(s) 18, 49, 117, 1951, 2149, 2252, 2887, 2993, 3157
HincII GTYRAC 4 cut(s) 294, 1234, 2338, 2587
HindII GTYRAC 4 cut(s) 294, 1234, 2338, 2587
HindIII AAGCTT 2 cut(s) 1586, 2024
HpaI GTTAAC 1 cut(s) 1234
Hpy166II GTNNAC 9 cut(s) 294, 1234, 1667, 1721, 2163, 2172, 2338, 2587, 2965
Hpy8I GTNNAC 9 cut(s) 294, 1234, 1667, 1721, 2163, 2172, 2338, 2587, 2965
Hpy99I CGWCG 8 cut(s) 1219, 1360, 1381, 2111, 2342, 2396, 2665, 2729
HpyCH4IV ACGT 9 cut(s) 1529, 1637, 1902, 2283, 2340, 2367, 2495, 2727, 3162
HpySE526I ACGT 9 cut(s) 1529, 1637, 1902, 2283, 2340, 2367, 2495, 2727, 3162
Hsp92I GRCGYC 2 cut(s) 1902, 2391
Hsp92II CATG 9 cut(s) 18, 49, 117, 1951, 2149, 2252, 2887, 2993, 3157
KflI GGGWCCC 2 cut(s) 166, 2244
Kpn2I TCCGGA 1 cut(s) 2069
KroI GCCGGC 3 cut(s) 602, 2707, 3226
KroNI GCCGGC 3 cut(s) 604, 2709, 3228
Ksp22I TGATCA 1 cut(s) 594
KspAI GTTAAC 1 cut(s) 1234
LguI GCTCTTC 2 cut(s) 1298, 3014
LweI GCATC 1 cut(s) 2205
MaeI CTAG 2 cut(s) 1898, 2793
MaeII ACGT 9 cut(s) 1529, 1637, 1902, 2283, 2340, 2367, 2495, 2727, 3162
MaeIII GTNAC 9 cut(s) 202, 275, 1254, 2302, 2404, 2516, 2529, 2562, 3262
MbiI CCGCTC 2 cut(s) 1761, 2385
MboII GAAGA 8 cut(s) 881, 1163, 1285, 2171, 2227, 3031, 3249, 3256
MfeI CAATTG 1 cut(s) 857
MflI RGATCY 4 cut(s) 423, 1465, 1506, 2111
MlsI TGGCCA 1 cut(s) 2144
MluNI TGGCCA 1 cut(s) 2144
MlyI GAGTC 4 cut(s) 924, 1250, 2007, 2226
MmeI TCCRAC 4 cut(s) 240, 1248, 1338, 1731
Mox20I TGGCCA 1 cut(s) 2144
Mph1103I ATGCAT 1 cut(s) 400
MroI TCCGGA 1 cut(s) 2069
MroNI GCCGGC 3 cut(s) 602, 2707, 3226
MroXI GAANNNNTTC 1 cut(s) 1868
MscI TGGCCA 1 cut(s) 2144
MseI TTAA 5 cut(s) 401, 869, 1233, 1268, 2354
MslI CAYNNNNRTG 2 cut(s) 968, 2150
Msp20I TGGCCA 1 cut(s) 2144
MspA1I CMGCKG 5 cut(s) 549, 1036, 1401, 3286, 3332
MspR9I CCNGG 9 cut(s) 809, 1284, 1572, 2225, 2261, 2647, 2848, 3134, 3203
MunI CAATTG 1 cut(s) 857
Mva1269I GAATGC 1 cut(s) 1543
MvaI CCWGG 1 cut(s) 1572
NaeI GCCGGC 3 cut(s) 604, 2709, 3228
NciI CCSGG 8 cut(s) 809, 1284, 2225, 2261, 2647, 2848, 3134, 3203
NcoI CCATGG 2 cut(s) 14, 2145
NgoMIV GCCGGC 3 cut(s) 602, 2707, 3226
NlaIII CATG 9 cut(s) 18, 49, 117, 1951, 2149, 2252, 2887, 2993, 3157
NmeAIII GCCGAG 3 cut(s) 125, 912, 2444
NmuCI GTSAC 4 cut(s) 202, 1254, 2404, 3262
NsbI TGCGCA 1 cut(s) 2623
NsiI ATGCAT 1 cut(s) 400
NspI RCATGY 3 cut(s) 1951, 2887, 2993
NspV TTCGAA 1 cut(s) 2021
PaeI GCATGC 1 cut(s) 2887
PaeR7I CTCGAG 2 cut(s) 443, 1238
PaqCI CACCTGC 1 cut(s) 215
PauI GCGCGC 1 cut(s) 2135
PciI ACATGT 1 cut(s) 2989
PciSI GCTCTTC 2 cut(s) 1298, 3014
PcsI WCGNNNNNNNCGW 2 cut(s) 1203, 1486
PctI GAATGC 1 cut(s) 1543
PdiI GCCGGC 3 cut(s) 604, 2709, 3228
PdmI GAANNNNTTC 1 cut(s) 1868
Pfl23II CGTACG 1 cut(s) 2524
PflMI CCANNNNNTGG 3 cut(s) 229, 716, 1606
PfoI TCCNGGA 2 cut(s) 807, 1282
PinAI ACCGGT 1 cut(s) 1127
PleI GAGTC 4 cut(s) 923, 1249, 2006, 2226
PpsI GAGTC 4 cut(s) 923, 1249, 2006, 2226
PpuMI RGGWCCY 4 cut(s) 166, 2244, 2850, 3031
PscI ACATGT 1 cut(s) 2989
Psp124BI GAGCTC 3 cut(s) 807, 933, 1110
Psp5II RGGWCCY 4 cut(s) 166, 2244, 2850, 3031
Psp6I CCWGG 1 cut(s) 1570
PspEI GGTNACC 1 cut(s) 202
PspFI CCCAGC 3 cut(s) 259, 670, 2749
PspGI CCWGG 1 cut(s) 1570
PspLI CGTACG 1 cut(s) 2524
PspOMI GGGCCC 3 cut(s) 2221, 2287, 3199
PspPPI RGGWCCY 4 cut(s) 166, 2244, 2850, 3031
PspXI VCTCGAGB 2 cut(s) 443, 1238
PsrI GAACNNNNNNTAC 2 cut(s) 2584, 2616
PstI CTGCAG 2 cut(s) 948, 1035
PstNI CAGNNNCTG 1 cut(s) 1030
PsuI RGATCY 4 cut(s) 423, 1465, 1506, 2111
PteI GCGCGC 1 cut(s) 2135
PvuII CAGCTG 3 cut(s) 1036, 3286, 3332
RsaI GTAC 4 cut(s) 248, 378, 2526, 2687
RsaNI GTAC 4 cut(s) 247, 377, 2525, 2686
RseI CAYNNNNRTG 2 cut(s) 968, 2150
SacI GAGCTC 3 cut(s) 807, 933, 1110
SalI GTCGAC 2 cut(s) 292, 2336
SapI GCTCTTC 2 cut(s) 1298, 3014
SaqAI TTAA 5 cut(s) 401, 869, 1233, 1268, 2354
SchI GAGTC 4 cut(s) 924, 1250, 2007, 2226
ScrFI CCNGG 9 cut(s) 809, 1284, 1572, 2225, 2261, 2647, 2848, 3134, 3203
SfaNI GCATC 1 cut(s) 2205
SfcI CTRYAG 3 cut(s) 944, 1031, 1742
Sfr274I CTCGAG 2 cut(s) 443, 1238
SfuI TTCGAA 1 cut(s) 2021
SlaI CTCGAG 2 cut(s) 443, 1238
SmiMI CAYNNNNRTG 2 cut(s) 968, 2150
SmlI CTYRAG 4 cut(s) 385, 443, 725, 1238
SmoI CTYRAG 4 cut(s) 385, 443, 725, 1238
SphI GCATGC 1 cut(s) 2887
SspI AATATT 1 cut(s) 1783
SspMI CTAG 2 cut(s) 1898, 2793
SstI GAGCTC 3 cut(s) 807, 933, 1110
StyD4I CCNGG 9 cut(s) 807, 1282, 1570, 2223, 2259, 2645, 2846, 3132, 3201
StyI CCWWGG 2 cut(s) 14, 2145
TaiI ACGT 9 cut(s) 1532, 1640, 1905, 2286, 2343, 2370, 2498, 2730, 3165
TaqII GACCGA 1 cut(s) 835
TauI GCSGC 9 cut(s) 77, 552, 1404, 1432, 2093, 2121, 2124, 2385, 3170
Tru1I TTAA 5 cut(s) 401, 869, 1233, 1268, 2354
Tru9I TTAA 5 cut(s) 401, 869, 1233, 1268, 2354
TscAI CASTG 6 cut(s) 1665, 2037, 2087, 2902, 2967, 3005
TseFI GTSAC 4 cut(s) 202, 1254, 2404, 3262
Tsp45I GTSAC 4 cut(s) 202, 1254, 2404, 3262
TspDTI ATGAA 4 cut(s) 34, 986, 3170, 3240
TspGWI ACGGA 5 cut(s) 1234, 1344, 2124, 2435, 2439
TspRI CASTG 6 cut(s) 1665, 2037, 2087, 2902, 2967, 3005
Van91I CCANNNNNTGG 3 cut(s) 229, 716, 1606
VneI GTGCAC 2 cut(s) 2161, 2170
XapI RAATTY 7 cut(s) 238, 829, 1350, 1441, 1456, 3183, 3247
XbaI TCTAGA 1 cut(s) 1897
XceI RCATGY 3 cut(s) 1951, 2887, 2993
XcmI CCANNNNNNNNNTGG 3 cut(s) 151, 1681, 3160
XhoI CTCGAG 2 cut(s) 443, 1238
XmiI GTMKAC 2 cut(s) 293, 2337
XmnI GAANNNNTTC 1 cut(s) 1868
XspI CTAG 2 cut(s) 1898, 2793
ZraI GACGTC 1 cut(s) 1903
Zsp2I ATGCAT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.