pycom06g15270

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012442
Physical Location & Seq
Reverse (-)
19828900 .. 19829583
684 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
ATGTGCCTTTTCCTGGATTGGAACCCTTCAGCCACTTCTATCACCGTGGGGCTTTCGAATGGCTTTGTCGCGATAGCTTCAATTGGGGAATCCCAAATTGAGACACAAGAAACATGGAAAGCGCACGATTTTGAGGTTTGGACAGCTTCCTATGATATCCACCAGCCCCAGTTGGTGTACAGTGGTTCAGATGACTGCAAATTCAAATGCTGGGATTTGCGAGACGGTCCTTCGAAGTTGGCATTTCAGAACACCAAGGCCCATACAATGGGAGTTTGCTGCATAGCCAAAAATCCCTACGATTCGAATAGTGTAGTTACTGGCAGCTATGATGAGTACCTGAGGTTGTGGGATGTAAGATCAATATCAACACCAGTTAATGAAACCTCTGTTTGCTTAGGGGGAGGAGTGTGGAGGATCAAGTTCCATCCTTTTGTGGCCGGTCTTGTCTTGACAGCTTGTATGCACAACGGATTTTCGATAGTCAACATTAATGAGGATAAAACTGAAGTGATTGAAACGTACAGCAAGCACGAGTCACTTGCATATGGAGCTGACTGGCACAGAGACAAATCGTTCCATGAAGGCAAAAGGAATAGCACTTTAGTTGCTACTTGCTCATTTTATGACCGTCTTCTTCGTTTATGGATGCCGGAAGGTGATTTGCAGGATTCTGTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

228

Amino Acids

25.55

Weight (kDa)

5.29

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 5 - 76 9.1e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 5 - 161 3.5e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 6 - 145 4.7e-12 WDR3 first beta-propeller domain
WDR55 PF24796 6 - 180 3.2e-09 WDR55
Beta-prop_WDR5 PF25175 12 - 121 8.5e-12 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 13 - 138 2.3e-06 TEP-1 second beta-propeller
Beta-prop_EIPR1 PF23609 27 - 154 1.6e-08 EIPR1 beta-propeller
WD40_Prp19 PF24814 31 - 144 5e-06 Prp19 WD40 domain
WD40_Prp19 PF24814 51 - 190 7.1e-07 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 83 - 192 6.2e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012442)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63010
fragaria_vesca FvH4_5g05540 FvH4_5g05540
malus_domestica MD06G1171600.v1.1
prunus_persica Prupe.5G171000_v2.0.a1 Prupe.5G171000_v2.0.a1
pyrus_communis pycom06g15270
rosa_chinensis RchiOBHm_Chr7g0193241
rosa_laevigata RLG00000004284
rosa_multiflora Rmu_ssc0000092.1_g000021
rosa_roxburghii Rroxscaffold_3G00262260
rosa_rugosa Rorug07G0008400 Rorug07G0008400 Rorug07G0008400
rosa_samantha Rh7AG134000 Rh7BG135000 Rh7CG138000 Rh7DG136000
rosa_wichuraiana Rw7G011460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 268
AccII CGCG 1 cut(s) 71
AclWI GGATC 1 cut(s) 425
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 2 cut(s) 12, 528
AfaI GTAC 3 cut(s) 179, 338, 524
AfiI CCNNNNNNNGG 2 cut(s) 13, 268
AgsI TTSAA 3 cut(s) 81, 205, 518
AjnI CCWGG 1 cut(s) 12
AluBI AGCT 5 cut(s) 77, 146, 327, 458, 554
AluI AGCT 5 cut(s) 77, 146, 327, 458, 554
Alw26I GTCTC 3 cut(s) 95, 216, 561
AlwI GGATC 1 cut(s) 425
AoxI GGCC 2 cut(s) 258, 438
ApeKI GCWGC 2 cut(s) 279, 324
ApoI RAATTY 1 cut(s) 200
AseI ATTAAT 1 cut(s) 492
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 2 cut(s) 227, 259
AsuHPI GGTGA 2 cut(s) 34, 671
AsuII TTCGAA 3 cut(s) 56, 233, 305
AvaII GGWCC 1 cut(s) 227
AxyI CCTNAGG 1 cut(s) 341
BauI CACGAG 1 cut(s) 533
BbsI GAAGAC 1 cut(s) 626
BbvI GCAGC 2 cut(s) 266, 336
BccI CCATC 1 cut(s) 435
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 1 cut(s) 14
BcoDI GTCTC 3 cut(s) 95, 216, 561
BisI GCNGC 2 cut(s) 280, 325
BlsI GCNGC 2 cut(s) 281, 326
Bme1390I CCNGG 1 cut(s) 14
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 227, 259
BmiI GGNNCC 1 cut(s) 23
BmrFI CCNGG 1 cut(s) 14
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 1 cut(s) 639
BmuI ACTGGG 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 626
Bpu10I CCTNAGC 1 cut(s) 397
Bpu14I TTCGAA 3 cut(s) 56, 233, 305
BsaBI GATNNNNATC 1 cut(s) 364
BsaJI CCNNGG 2 cut(s) 45, 255
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 268
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 4 cut(s) 169, 325, 374, 563
Bse21I CCTNAGG 1 cut(s) 341
Bse8I GATNNNNATC 1 cut(s) 364
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 45, 255
BseGI GGATG 3 cut(s) 358, 427, 654
BseJI GATNNNNATC 1 cut(s) 364
BseLI CCNNNNNNNGG 2 cut(s) 13, 268
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 4 cut(s) 169, 325, 374, 563
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 2 cut(s) 266, 336
BseYI CCCAGC 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 2 cut(s) 260, 440
BsiSI CCGG 2 cut(s) 441, 653
BslI CCNNNNNNNGG 2 cut(s) 13, 268
BsmAI GTCTC 3 cut(s) 95, 216, 561
BsmBI CGTCTC 1 cut(s) 216
BsnI GGCC 2 cut(s) 260, 440
Bsp119I TTCGAA 3 cut(s) 56, 233, 305
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 2 cut(s) 359, 417
Bsp68I TCGCGA 1 cut(s) 71
BspANI GGCC 2 cut(s) 260, 440
BspCNI CTCAG 1 cut(s) 333
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 1 cut(s) 23
BspPI GGATC 1 cut(s) 425
BspT104I TTCGAA 3 cut(s) 56, 233, 305
BsrFI RCCGGY 1 cut(s) 440
BsrGI TGTACA 1 cut(s) 177
BsrI ACTGG 4 cut(s) 169, 325, 374, 563
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 2 cut(s) 45, 255
BssMI GATC 2 cut(s) 359, 417
BssSI CACGAG 1 cut(s) 533
BssT1I CCWWGG 1 cut(s) 255
Bst2BI CACGAG 1 cut(s) 533
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 4 cut(s) 46, 182, 227, 632
BstAUI TGTACA 1 cut(s) 177
BstBI TTCGAA 3 cut(s) 56, 233, 305
BstC8I GCNNGC 1 cut(s) 530
BstDEI CTNAG 2 cut(s) 341, 397
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 3 cut(s) 358, 427, 654
BstFNI CGCG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 2 cut(s) 362, 420
BstMAI GTCTC 3 cut(s) 95, 216, 561
BstMBI GATC 2 cut(s) 359, 417
BstMWI GCNNNNNNNGC 1 cut(s) 551
BstNI CCWGG 1 cut(s) 14
BstSCI CCNGG 1 cut(s) 12
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 266, 336
BstV2I GAAGAC 1 cut(s) 626
Bsu36I CCTNAGG 1 cut(s) 341
BsuRI GGCC 2 cut(s) 260, 440
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 3 cut(s) 358, 427, 654
BtsIMutI CAGTG 1 cut(s) 187
BtuMI TCGCGA 1 cut(s) 71
Cac8I GCNNGC 1 cut(s) 530
CfoI GCGC 1 cut(s) 124
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 2 cut(s) 227, 259
Csp6I GTAC 3 cut(s) 178, 337, 523
CviAII CATG 2 cut(s) 114, 581
CviQI GTAC 3 cut(s) 178, 337, 523
DdeI CTNAG 2 cut(s) 341, 397
DpnI GATC 2 cut(s) 361, 419
DpnII GATC 2 cut(s) 359, 417
EaeI YGGCCR 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 255
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 2 cut(s) 12, 528
Eco81I CCTNAGG 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 12
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 216
FaeI CATG 2 cut(s) 117, 584
FatI CATG 2 cut(s) 113, 580
FauNDI CATATG 1 cut(s) 547
Fnu4HI GCNGC 2 cut(s) 280, 325
FokI GGATG 3 cut(s) 365, 414, 661
Fsp4HI GCNGC 2 cut(s) 280, 325
GlaI GCGC 1 cut(s) 123
GluI GCNGC 2 cut(s) 280, 325
GsaI CCCAGC 1 cut(s) 214
HaeIII GGCC 2 cut(s) 260, 440
HapII CCGG 2 cut(s) 441, 653
HhaI GCGC 1 cut(s) 124
Hin1II CATG 2 cut(s) 117, 584
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HinfI GANTC 4 cut(s) 89, 302, 536, 671
HpaII CCGG 2 cut(s) 441, 653
HphI GGTGA 2 cut(s) 34, 671
Hpy166II GTNNAC 2 cut(s) 178, 487
Hpy188I TCNGA 2 cut(s) 190, 249
Hpy188III TCNNGA 2 cut(s) 70, 451
Hpy8I GTNNAC 2 cut(s) 178, 487
HpyAV CCTTC 4 cut(s) 36, 240, 578, 650
HpyCH4III ACNGT 4 cut(s) 46, 182, 227, 632
HpyCH4IV ACGT 1 cut(s) 521
HpyCH4V TGCA 5 cut(s) 198, 282, 466, 545, 667
HpyF10VI GCNNNNNNNGC 1 cut(s) 551
HpyF3I CTNAG 2 cut(s) 341, 397
HpySE526I ACGT 1 cut(s) 521
Hsp92II CATG 2 cut(s) 117, 584
HspAI GCGC 1 cut(s) 122
Kzo9I GATC 2 cut(s) 359, 417
LmnI GCTCC 1 cut(s) 551
Lsp1109I GCAGC 2 cut(s) 266, 336
LweI GCATC 1 cut(s) 639
MaeII ACGT 1 cut(s) 521
MaeIII GTNAC 2 cut(s) 316, 537
MalI GATC 2 cut(s) 361, 419
MboI GATC 2 cut(s) 359, 417
MboII GAAGA 2 cut(s) 626, 629
MfeI CAATTG 1 cut(s) 81
MluCI AATT 3 cut(s) 81, 96, 200
MlyI GAGTC 1 cut(s) 545
MnlI CCTC 6 cut(s) 127, 336, 397, 398, 408, 490
MseI TTAA 2 cut(s) 378, 492
MspI CCGG 2 cut(s) 441, 653
MspR9I CCNGG 1 cut(s) 14
MunI CAATTG 1 cut(s) 81
MvaI CCWGG 1 cut(s) 14
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 551
NdeI CATATG 1 cut(s) 547
NdeII GATC 2 cut(s) 359, 417
NlaIII CATG 2 cut(s) 117, 584
NlaIV GGNNCC 1 cut(s) 23
NmuCI GTSAC 1 cut(s) 537
NruI TCGCGA 1 cut(s) 71
NspV TTCGAA 3 cut(s) 56, 233, 305
PfeI GAWTC 3 cut(s) 89, 302, 671
PflMI CCANNNNNTGG 1 cut(s) 268
PfoI TCCNGGA 1 cut(s) 12
PkrI GCNGC 2 cut(s) 281, 326
PleI GAGTC 1 cut(s) 544
PpsI GAGTC 1 cut(s) 544
PshBI ATTAAT 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 12
PspFI CCCAGC 1 cut(s) 210
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 23
PspPI GGNCC 2 cut(s) 227, 259
RruI TCGCGA 1 cut(s) 71
RsaI GTAC 3 cut(s) 179, 338, 524
RsaNI GTAC 3 cut(s) 178, 337, 523
SaqAI TTAA 2 cut(s) 378, 492
SatI GCNGC 2 cut(s) 280, 325
Sau3AI GATC 2 cut(s) 359, 417
Sau96I GGNCC 2 cut(s) 227, 259
SchI GAGTC 1 cut(s) 545
ScrFI CCNGG 1 cut(s) 14
SfaNI GCATC 1 cut(s) 639
SfuI TTCGAA 3 cut(s) 56, 233, 305
SinI GGWCC 1 cut(s) 227
Sse9I AATT 3 cut(s) 81, 96, 200
StyD4I CCNGG 1 cut(s) 12
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 4 cut(s) 46, 182, 227, 632
TaiI ACGT 1 cut(s) 524
TaqI TCGA 4 cut(s) 56, 233, 305, 479
TasI AATT 3 cut(s) 81, 96, 200
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 3 cut(s) 89, 302, 671
Tru1I TTAA 2 cut(s) 378, 492
Tru9I TTAA 2 cut(s) 378, 492
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 537
TseI GCWGC 2 cut(s) 279, 324
Tsp45I GTSAC 1 cut(s) 537
TspDTI ATGAA 2 cut(s) 396, 597
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 1 cut(s) 187
Van91I CCANNNNNTGG 1 cut(s) 268
VpaK11BI GGWCC 1 cut(s) 227
VspI ATTAAT 1 cut(s) 492
XapI RAATTY 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.