pycom06g16020

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0015017
Physical Location & Seq
Reverse (-)
20487182 .. 20488381
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1200 bp
ATGGAATGCTACTCATCCGATCAGTACCCAGCCAATGACCTCTCTGACCAATCAGAGTTTCCCTACATCTCTCAGCCAAGTTTCCAGTCAGTTCCTTCACTCAACTCACAAAACAACTTTCCAACAAACACTTACCACCACTGCCTCACCACCTTGCAAGGCCACAACTCCTACATATCCTCTCTAACCCTAGCCGGAAACTTCATCTACACCGGCTCGTCCGATAGAGAAATCCGGTCTTGGAGGTGCAACCCTTTACTGGAATTCGACAACGAAAACACTACTCATCATGATCACAACATGGTGGCGGCAGGAAAGGGTGCAGTGAAGTCCCTGGCTATTTCGGGTGACAAGCTCTTCAGTGCTCATCAGGACCACAAGATTCGAGTGTGGAAAATCGACAGCCAGGAGCCCGATCTCCAAAAGTGCTCGCGGCTAGCCACGCTGCCAACGCTTGGTGACCGCGCTTTGAAGCTCCTGATGCCAAAGAACCATGTCCAAATAAGAAGGCACAAGAAGCGCACTTGGGTGCATCATGTTGACACAGTGTCTGCCCTAGCCTTGTCAAGCGATGAGCTGCTGCTATACTCGGTTTCATGGGACCGTACACTCAAGGTTTGGAGCACAGCAGACTTCAGATGCTTGGAGTCTGTGAGAAATGCACATGATGATGCTATAAACGCAGTAGCATCGTCTAATGATGGATATGTTTACACTGGATCAGCAGACAAAAGAATTAAGGTTTGGAAAAAGGACTTCGGAGAGAAAAAGCACTTTTTAGTTGCTACACTGGAAAGGCACAATTCTGGAGTGAACGCACTAGCTCTGAGCAGTGATGGGTGTGTGTTGTATTCTGGTGCATGTGACAGTTCCATTGTGGTTTGGAAGAATAAAGATGGGGGTGGGAATATGGAAGTTGTGGGTGTGCTTAGAGGTCATACCAAGTCAATATTGTGCTTGGCTGTGGCTGCAGACTTGGTGTGCAGTGGCTCTGCAGATAAAAGTATAAGAATTTGGAGAGGGGTTAATAAAAGTTACTCATGTTTAGCAGTTTTGGAAGGGCATAAAGGTCCAGTTAAGTGTTTAACTGCTGCAATTGATGACCTTTGCAGCTCATCTGACTCCTCTTGTGTTATTTACAGTGGTGGTTTGGACTGTGACATCAAAGTCTGGCAGGTCTTTGTTCCTAGTGACCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

400

Amino Acids

44.02

Weight (kDa)

6.97

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_WDHD1_1st PF24817 64 - 218 1.1e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 109 - 295 2.8e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 154 - 362 1.1e-22 THOC3 beta-propeller domain
WD40_Gbeta PF25391 172 - 339 3.6e-13 G protein beta WD-40 repeat protein
WDR55 PF24796 172 - 296 2.1e-09 WDR55
Beta-prop_WDR5 PF25175 178 - 255 5.8e-11 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 179 - 250 1.4e-08 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 180 - 298 2.4e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 183 - 298 1.7e-14 WDR3 second beta-propeller domain
NBCH_WD40 PF20426 183 - 251 5.9e-06 Neurobeachin beta propeller domain
Beta-prop_EML_2 PF23414 184 - 323 1.2e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML PF23409 203 - 292 1.9e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 220 - 393 1.7e-24 WDR5 beta-propeller domain
Beta-prop_WDR75_1st PF23869 226 - 299 1.2e-08 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR3_2nd PF25172 234 - 341 3.8e-15 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 239 - 377 1.7e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 257 - 393 1.6e-15 WDR3 first beta-propeller domain
WD40 PF00400 261 - 295 5.7e-07 WD domain, G-beta repeat
EIF3I PF24805 264 - 340 1.7e-06 EIF3I
WD40_Prp19 PF24814 268 - 364 1.1e-12 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 273 - 396 1.8e-09 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50120
fragaria_vesca FvH4_5g16381
malus_domestica MD06G1181200.v1.1 MD14G1187000.v1.1
prunus_persica Prupe.5G179200_v2.0.a1
pyrus_communis pycom06g16020
rosa_chinensis RchiOBHm_Chr7g0179711
rosa_laevigata RLG00000005328
rosa_multiflora Rmu_sc0005329.1_g000037
rosa_roxburghii Rroxscaffold_155G00443790 Rroxscaffold_3G00273530
rosa_samantha Rh7AG027000 Rh7BG026100 Rh7CG028100 Rh7DG027700
rosa_wichuraiana Rw7G002170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1166
Acc36I ACCTGC 1 cut(s) 1165
AccB7I CCANNNNNTGG 1 cut(s) 455
AccII CGCG 2 cut(s) 433, 465
AciI CCGC 3 cut(s) 308, 433, 463
AclWI GGATC 1 cut(s) 727
AcsI RAATTY 2 cut(s) 263, 1011
AcuI CTGAAG 2 cut(s) 343, 619
AfaI GTAC 2 cut(s) 26, 607
AfiI CCNNNNNNNGG 2 cut(s) 259, 455
AgsI TTSAA 1 cut(s) 472
AhdI GACNNNNNGTC 1 cut(s) 547
AjnI CCWGG 2 cut(s) 333, 405
AjuI GAANNNNNNNTTGG 4 cut(s) 42, 74, 727, 759
AleI CACNNNNGTG 1 cut(s) 527
AluBI AGCT 5 cut(s) 355, 475, 577, 824, 1113
AluI AGCT 5 cut(s) 355, 475, 577, 824, 1113
Alw21I GWGCWC 3 cut(s) 367, 431, 626
AlwI GGATC 1 cut(s) 727
AlwNI CAGNNNCTG 1 cut(s) 551
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 6 cut(s) 445, 577, 580, 968, 1091, 1110
ApoI RAATTY 2 cut(s) 263, 1011
AspLEI GCGC 2 cut(s) 467, 522
AspS9I GGNCC 3 cut(s) 373, 601, 1070
AsuHPI GGTGA 3 cut(s) 139, 359, 470
AsuNHI GCTAGC 1 cut(s) 436
AvaII GGWCC 3 cut(s) 373, 601, 1070
BanII GRGCYC 1 cut(s) 414
Bbv12I GWGCWC 3 cut(s) 367, 431, 626
BbvI GCAGC 6 cut(s) 432, 564, 567, 955, 1078, 1122
BccI CCATC 3 cut(s) 695, 830, 890
BcgI CGANNNNNNTGC 2 cut(s) 672, 706
BciT130I CCWGG 2 cut(s) 335, 407
BclI TGATCA 1 cut(s) 292
BfaI CTAG 5 cut(s) 191, 437, 557, 821, 1188
BfmI CTRYAG 2 cut(s) 969, 993
BfuAI ACCTGC 1 cut(s) 1165
BisI GCNGC 8 cut(s) 309, 434, 446, 578, 581, 969, 1092, 1111
BlsI GCNGC 8 cut(s) 310, 435, 447, 579, 582, 970, 1093, 1112
Bme1390I CCNGG 2 cut(s) 335, 407
Bme18I GGWCC 3 cut(s) 373, 601, 1070
BmeRI GACNNNNNGTC 1 cut(s) 547
BmgT120I GGNCC 3 cut(s) 373, 601, 1070
BmiI GGNNCC 2 cut(s) 411, 602
BmrFI CCNGG 2 cut(s) 335, 407
BmsI GCATC 5 cut(s) 471, 541, 629, 661, 698
BmtI GCTAGC 1 cut(s) 440
BpmI CTGGAG 1 cut(s) 828
BpuEI CTTGAG 1 cut(s) 596
BsaJI CCNNGG 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 234
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 455
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 5 cut(s) 85, 264, 721, 795, 1073
BseBI CCWGG 2 cut(s) 335, 407
BseDI CCNNGG 1 cut(s) 333
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 2 cut(s) 259, 455
BseMII CTCAG 2 cut(s) 86, 818
BseNI ACTGG 5 cut(s) 85, 264, 721, 795, 1073
BseRI GAGGAG 1 cut(s) 1114
BseXI GCAGC 6 cut(s) 432, 564, 567, 955, 1078, 1122
BseYI CCCAGC 1 cut(s) 28
BsgI GTGCAG 2 cut(s) 342, 1003
Bsh1236I CGCG 2 cut(s) 433, 465
BshFI GGCC 1 cut(s) 162
BsiHKAI GWGCWC 3 cut(s) 367, 431, 626
BsiSI CCGG 3 cut(s) 195, 213, 235
BslFI GGGAC 2 cut(s) 316, 614
BslI CCNNNNNNNGG 2 cut(s) 259, 455
BsmFI GGGAC 2 cut(s) 316, 614
BsmI GAATGC 1 cut(s) 11
BsnI GGCC 1 cut(s) 162
Bsp1286I GDGCHC 4 cut(s) 367, 414, 431, 626
Bsp143I GATC 4 cut(s) 19, 292, 415, 719
BspACI CCGC 3 cut(s) 308, 433, 463
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 2 cut(s) 85, 819
BspFNI CGCG 2 cut(s) 433, 465
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 411, 602
BspMAI CTGCAG 2 cut(s) 973, 997
BspMI ACCTGC 1 cut(s) 1165
BspOI GCTAGC 1 cut(s) 440
BspPI GGATC 1 cut(s) 727
BspQI GCTCTTC 1 cut(s) 362
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 5 cut(s) 85, 264, 721, 795, 1073
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 4 cut(s) 19, 292, 415, 719
Bst2UI CCWGG 2 cut(s) 335, 407
Bst4CI ACNGT 5 cut(s) 547, 605, 869, 1142, 1157
Bst6I CTCTTC 1 cut(s) 362
BstC8I GCNNGC 2 cut(s) 431, 438
BstDEI CTNAG 3 cut(s) 72, 827, 929
BstEII GGTNACC 1 cut(s) 458
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 2 cut(s) 433, 465
BstHHI GCGC 2 cut(s) 467, 522
BstKTI GATC 4 cut(s) 22, 295, 418, 722
BstMBI GATC 4 cut(s) 19, 292, 415, 719
BstMWI GCNNNNNNNGC 6 cut(s) 442, 451, 481, 517, 680, 968
BstNI CCWGG 2 cut(s) 335, 407
BstNSI RCATGY 1 cut(s) 864
BstPI GGTNACC 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 333, 405
BstSFI CTRYAG 2 cut(s) 969, 993
BstUI CGCG 2 cut(s) 433, 465
BstV1I GCAGC 6 cut(s) 432, 564, 567, 955, 1078, 1122
BsuRI GGCC 1 cut(s) 162
BtgZI GCGATG 1 cut(s) 585
BtsCI GGATG 1 cut(s) 14
BtsI GCAGTG 4 cut(s) 139, 330, 838, 991
BtsIMutI CAGTG 9 cut(s) 139, 330, 367, 552, 714, 788, 838, 991, 1147
BveI ACCTGC 1 cut(s) 1165
Cac8I GCNNGC 2 cut(s) 431, 438
CaiI CAGNNNCTG 1 cut(s) 551
CciI TCATGA 1 cut(s) 289
CfoI GCGC 2 cut(s) 467, 522
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 3 cut(s) 373, 601, 1070
Csp6I GTAC 2 cut(s) 25, 606
CviAII CATG 8 cut(s) 290, 301, 494, 536, 597, 665, 861, 1041
CviQI GTAC 2 cut(s) 25, 606
DdeI CTNAG 3 cut(s) 72, 827, 929
DpnI GATC 4 cut(s) 21, 294, 417, 721
DpnII GATC 4 cut(s) 19, 292, 415, 719
DrdI GACNNNNNNGTC 1 cut(s) 1166
DriI GACNNNNNGTC 1 cut(s) 547
DseDI GACNNNNNNGTC 1 cut(s) 1166
Eam1104I CTCTTC 1 cut(s) 362
Eam1105I GACNNNNNGTC 1 cut(s) 547
EarI CTCTTC 1 cut(s) 362
Eco24I GRGCYC 1 cut(s) 414
Eco47I GGWCC 3 cut(s) 373, 601, 1070
Eco57I CTGAAG 2 cut(s) 343, 619
Eco91I GGTNACC 1 cut(s) 458
EcoO65I GGTNACC 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 263
EcoRII CCWGG 2 cut(s) 333, 405
EcoT38I GRGCYC 1 cut(s) 414
FaeI CATG 8 cut(s) 293, 304, 497, 539, 600, 668, 864, 1044
FaqI GGGAC 2 cut(s) 316, 614
FatI CATG 8 cut(s) 289, 300, 493, 535, 596, 664, 860, 1040
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 8 cut(s) 309, 434, 446, 578, 581, 969, 1092, 1111
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 8 cut(s) 309, 434, 446, 578, 581, 969, 1092, 1111
FspBI CTAG 5 cut(s) 191, 437, 557, 821, 1188
GlaI GCGC 2 cut(s) 466, 521
GluI GCNGC 8 cut(s) 309, 434, 446, 578, 581, 969, 1092, 1111
GsaI CCCAGC 1 cut(s) 32
GsuI CTGGAG 1 cut(s) 828
HaeIII GGCC 1 cut(s) 162
HapII CCGG 3 cut(s) 195, 213, 235
HhaI GCGC 2 cut(s) 467, 522
Hin1II CATG 8 cut(s) 293, 304, 497, 539, 600, 668, 864, 1044
Hin6I GCGC 2 cut(s) 465, 520
HinP1I GCGC 2 cut(s) 465, 520
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 3 cut(s) 382, 647, 1121
HpaII CCGG 3 cut(s) 195, 213, 235
HphI GGTGA 3 cut(s) 139, 359, 470
Hpy166II GTNNAC 4 cut(s) 541, 608, 712, 814
Hpy188I TCNGA 8 cut(s) 19, 46, 55, 223, 638, 761, 828, 1120
Hpy188III TCNNGA 4 cut(s) 290, 371, 478, 807
Hpy8I GTNNAC 4 cut(s) 541, 608, 712, 814
HpyAV CCTTC 3 cut(s) 105, 501, 1052
HpyCH4III ACNGT 5 cut(s) 547, 605, 869, 1142, 1157
HpyF10VI GCNNNNNNNGC 6 cut(s) 442, 451, 481, 517, 680, 968
HpyF3I CTNAG 3 cut(s) 72, 827, 929
Hsp92II CATG 8 cut(s) 293, 304, 497, 539, 600, 668, 864, 1044
HspAI GCGC 2 cut(s) 465, 520
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 4 cut(s) 19, 292, 415, 719
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 3 cut(s) 409, 480, 621
Lsp1109I GCAGC 6 cut(s) 432, 564, 567, 955, 1078, 1122
LweI GCATC 5 cut(s) 471, 541, 629, 661, 698
MaeI CTAG 5 cut(s) 191, 437, 557, 821, 1188
MaeIII GTNAC 6 cut(s) 347, 458, 863, 1034, 1157, 1190
MalI GATC 4 cut(s) 21, 294, 417, 721
MboI GATC 4 cut(s) 19, 292, 415, 719
MboII GAAGA 2 cut(s) 349, 898
MfeI CAATTG 1 cut(s) 1095
MhlI GDGCHC 4 cut(s) 367, 414, 431, 626
MluCI AATT 5 cut(s) 263, 735, 802, 1011, 1095
MlyI GAGTC 2 cut(s) 656, 1115
MmeI TCCRAC 1 cut(s) 146
MnlI CCTC 7 cut(s) 50, 155, 190, 237, 926, 1013, 1135
MseI TTAA 5 cut(s) 738, 1026, 1077, 1085, 1198
MslI CAYNNNNRTG 2 cut(s) 527, 669
MspI CCGG 3 cut(s) 195, 213, 235
MspR9I CCNGG 2 cut(s) 335, 407
MunI CAATTG 1 cut(s) 1095
Mva1269I GAATGC 1 cut(s) 11
MvaI CCWGG 2 cut(s) 335, 407
MvnI CGCG 2 cut(s) 433, 465
MwoI GCNNNNNNNGC 6 cut(s) 442, 451, 481, 517, 680, 968
NdeII GATC 4 cut(s) 19, 292, 415, 719
NheI GCTAGC 1 cut(s) 436
NlaIII CATG 8 cut(s) 293, 304, 497, 539, 600, 668, 864, 1044
NlaIV GGNNCC 2 cut(s) 411, 602
NmuCI GTSAC 5 cut(s) 347, 458, 863, 1157, 1190
NspI RCATGY 1 cut(s) 864
OliI CACNNNNGTG 1 cut(s) 527
PagI TCATGA 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 362
PctI GAATGC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 382
PflMI CCANNNNNTGG 1 cut(s) 455
PkrI GCNGC 8 cut(s) 310, 435, 447, 579, 582, 970, 1093, 1112
PleI GAGTC 2 cut(s) 655, 1115
PpsI GAGTC 2 cut(s) 655, 1115
Psp6I CCWGG 2 cut(s) 333, 405
PspEI GGTNACC 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 2 cut(s) 333, 405
PspN4I GGNNCC 2 cut(s) 411, 602
PspPI GGNCC 3 cut(s) 373, 601, 1070
PstI CTGCAG 2 cut(s) 973, 997
PstNI CAGNNNCTG 1 cut(s) 551
RsaI GTAC 2 cut(s) 26, 607
RsaNI GTAC 2 cut(s) 25, 606
RseI CAYNNNNRTG 2 cut(s) 527, 669
SapI GCTCTTC 1 cut(s) 362
SaqAI TTAA 5 cut(s) 738, 1026, 1077, 1085, 1198
SatI GCNGC 8 cut(s) 309, 434, 446, 578, 581, 969, 1092, 1111
Sau3AI GATC 4 cut(s) 19, 292, 415, 719
Sau96I GGNCC 3 cut(s) 373, 601, 1070
SchI GAGTC 2 cut(s) 656, 1115
ScrFI CCNGG 2 cut(s) 335, 407
SduI GDGCHC 4 cut(s) 367, 414, 431, 626
SfaNI GCATC 5 cut(s) 471, 541, 629, 661, 698
SfcI CTRYAG 2 cut(s) 969, 993
SinI GGWCC 3 cut(s) 373, 601, 1070
SmiMI CAYNNNNRTG 2 cut(s) 527, 669
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 5 cut(s) 263, 735, 802, 1011, 1095
SsiI CCGC 3 cut(s) 308, 433, 463
SspI AATATT 1 cut(s) 951
SspMI CTAG 5 cut(s) 191, 437, 557, 821, 1188
StyD4I CCNGG 2 cut(s) 333, 405
TaaI ACNGT 5 cut(s) 547, 605, 869, 1142, 1157
TaqI TCGA 3 cut(s) 267, 385, 399
TasI AATT 5 cut(s) 263, 735, 802, 1011, 1095
TauI GCSGC 2 cut(s) 311, 436
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 5 cut(s) 738, 1026, 1077, 1085, 1198
Tru9I TTAA 5 cut(s) 738, 1026, 1077, 1085, 1198
TscAI CASTG 9 cut(s) 146, 330, 367, 552, 721, 795, 838, 991, 1147
TseFI GTSAC 5 cut(s) 347, 458, 863, 1157, 1190
TseI GCWGC 6 cut(s) 445, 577, 580, 968, 1091, 1110
Tsp45I GTSAC 5 cut(s) 347, 458, 863, 1157, 1190
TspDTI ATGAA 2 cut(s) 193, 585
TspRI CASTG 9 cut(s) 146, 330, 367, 552, 721, 795, 838, 991, 1147
Van91I CCANNNNNTGG 1 cut(s) 455
VpaK11BI GGWCC 3 cut(s) 373, 601, 1070
XapI RAATTY 2 cut(s) 263, 1011
XceI RCATGY 1 cut(s) 864
XspI CTAG 5 cut(s) 191, 437, 557, 821, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.