pycom06g17800

Activating signal cointegrator 1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008552
Physical Location & Seq
Reverse (-)
21718371 .. 21733574
15204 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5634 bp
ATGATCGATCAACCTACTTCCAATGGACAAAGCTTCAACTTGAGTTGGTTACAAGATGCATGTGATCGGATAATAACAAAAAGTACTTCACAGCTTTCTGGAGATGAATTGGCTATGGCCATCTGTCGGGTTCTTGACTCTGACAAATCTGGTGATGAGATAGCAGGTGACTTGTTAGATCTTGTTGGGGATAGTGCGTTTGAAACTGTCCAAGACCTCATATCACATCGAAAGGAACTCGTCGATGCCATCCATCAAGGGCTACTGGGACTGAAATCTGATAAATTATCTTCAAGCTCTCAACCACGTATGCCTAGTTATGGCACACAGGTTACTGTTCAAACAGAATCTGAACGGCAAATTGATAAACTTCGGCGCAAAGAGGAAAAGCGACAAAGACGAGGAACAGAATATGGCGCTGATAGTGATCTGGCTGCTGTAAAATTTTCTTCTCTAGTACAAGCAAGTGAAAGAAAAAAACCTGTTGATGATCTCTTAGGGTATGGTGAAGCTCACTCTTTAGCAGTTAGTGCTCTTCCTCAAGGAACCGTGAGGAAGCATCATAAGGGGTATGAGGAAGTCATTATTCCCCCAACACCAACAGCACAAATGAAACCAGGAGAAAAGCTGATTGAGATAACAGAGTTAGATGAATTTGCTCAAGCTGCTTTTCGGGGATACAAATCTTTGAACCGTATTCAGAGCCGCATATTTCATACTGTTTATAACACTAATGAAAATATACTAGTTTGTGCTCCAACAGGAGCTGGTAAGACGAACATAGCTATGATTTCTATTCTACATGAGATTGGACAGCACTTCAAAGATGGTTACTTGCATAAAAATGAATTTAAAATAGTTTTTGTTGCACCTATGAAGGCATTAGCTTCAGAAGTCACATCAACTTTTAGCCATCGCTTATCTCCATTGAATATGACTGTGAAAGAACTTACTGGCGACATGCAACTATCTAAGAATGAACTTGAAGAAACTCAGATGATAGTTACTACACCTGAAAAATGGGATGTCATTACTCGCAAGAGCAGTGACATGGCACTTTCAATGCTCGTGAAGCTTTTAATTATTGATGAAGTGCATCTATTGAATGATGACAGGGGCCCTGTAATTGAGGCACTAGTTGCTCGGACTTTGCGCCAGGTGGAATCTACACAGACGATGATACGCATTGTTGGCCTTTCAGCAACTCTACCCAACTATTTGGAGGTTGCACAGTTTCTGAGAGTGAATCCAGATGCAGGACTCTTCTTTTTTGATGCTAGTTATCGTCCTGTGCCCCTTGCACAGCAGTATATTGGTATTAGTGAGCAGAACTTTGCAGCCCGAAATGAATTAATGAATGAGATATGCTACAAGAAGGTTGTTGAATCACTCAGACAAGGGTATCAGGCAATGGTATTCGTTCATTCTCGAAAGGACACAGCAAAAACAGCTCAGAAATTGGTTGAACTTGCTCGCAAATTTGAAGGACTGGATCTTTTCAAGAACGATGAACACCCACAGTTTTCATTGGTTCAGAGGGATGTTAAGAAGTCCAGAAACAAAGATCTTGTTGCACTTTTTGAATTTGGAGTTGGAGTTCATCATGCTGGGATGTTACGGTCTGATAGAGGTTTGACTGAGCGCCTTTTCTCTGATGGACTATTGAAGGTACTTGTTTGTACAGCAACTTTGGCATGGGGTGTAAATCTACCAGCTCACACTGTTGTGATAAAGGGGACTCAATTGTATGATCCAAAGGCTGGTGGGTGGAAAGACCTGGGCATGCTAGATGTTATGCAGATCTTCGGGCGTGCTGGGAGACCTCAATTCGACAAAAGTGGTGAGGGCATTATAATTACGTCTCACGACAAACTGGCCTACTATCTTCGGTTGCTGACAAGTCAACTTCCTATAGAAAGTCAGTTTATTAGTTCCTTGAAAGACAATTTAAATGCAGAGGTGGCACTTGGTACTGTCACCAATGTCAAGGAAGCTTGTGCATGGCTTGGATATACATACCTTTTTATAAGAATGAGGCTGAACCCTTTGGTGTACGGTATCGGGTGGGATGAGGTTGTTGCAGATCCTTCCTTGAGCTTGAAGCAAAGATCTCTTATTGCGGATGCTGCACGTTCCCTGGACAAAGCAAAAATGATGCGATTTGATGAAAAAAGTGGCAACTTTTATTGTACAGAACTTGGTCGAATTGCAAGCCACTTTTATATTCAATACTCAAGTGTTGAAACGTACAATGAATGTTTGAGACGCCACATGAATGAGACTGAGGTAATTGATATGGTAGCCCACTCCTCAGAATTTGAAAATATTGTTGTTCGAGATGAGGAACAGCATGAACTTGAGGCGTTGGTGCGATCATCATGCCCTTTAGAAGTTAAGGGGGGTCCTTCAAATAAACATGGAAAGATTTCCATTCTCATTCAGTTGTACATATCTCGTGGCTCAATCGATACCTTTTCCTTGGTTTCTGATGCTGCATATATAAGTGCAAGCTTGGCACGCATTATGCGGGCTTTATTTGAGATTTGCTTACGAAAGGGCTGGTCTGAGATGTCATTGTTCATGTTGGACTATTGTAAGGCTGTGGATCACCAAGTTTGGCCCCACCAGCATCCTCTCAGACAATTTGACAAGGATCTTTCAGGAGATATACTGCGAAAGCTTGAAGAACGAGAGGCTGACTTAGATCGCCTATATGAGATGGAGGAAAAAGACATTGGAGCATTGATTCGCTATGGGCCTGGAGGAAGGTTGGTCAAGCAATATTTGGGATATTTTCCATGGATCCAGTTGTCAGCAACTGTGAGTCCAATCACCAGAACTGTTTTAAAGGTGGACTTACTTATTACACCAGACTTCATATGGAAAGATCGTTTTCATGGCACAGCCCAACGTTGGTGGATTCTCGTTGAGGATTCGGAGAATGACCACATCTACCATTCAGAGCTATTCACTCTGACAAAGCGGATGGCTAAGGGGGAACCTCAGAAGCTTTCTTTTACTGTGCCAATATTTGAGCCACATCCACCACAGTATTATATCCGTGCTGTTTCGGATTCTTGGTTGCGCTCAGAGGCTTTCTACACCATATCTTTCCAGAATCTAGCACTGCCAGAGGCTCATACTTCACATACAGAACTGCTAGATTTGAAACCTCTCCCTGTGACTTCACTTGGCAACAATTCATACGAAGCATTGTACAGATTCTCACACTTTAATCCGATACAAACACAGACTTTCCATGTCTTGTATCACACAGACAATAATGTTTTGTTAGGAGCTCCCACTGGAAGTGGGAAAACTATATCTGCCGAGCTTGCTATGCTTCGTCTCTTCAATACCCAGCCTGATATGAAGGTCATTTACATAGCACCTTTGAAGGCTATTGTTCGGGAAAGAATGAATGATTGGAAAGGACGCCTCGTCTCTCAGCTAGGGAAAGAGATGGTTGAAATGACTGGTGATTATACTCCAGATATGATGGCCATTATGTCAGCGGATATCATAATATCTACTCCTGAGAAGTGGGATGGTATCAGTCGTAATTGGCATAGTCGAGACTATGTTAAGAAGGTTGGGCTTATGATCATGGATGAGATTCACTTGTTGGGAGCTGACCGTGGACCCATCCTTGAGGTTATTGTGTCAAGGATGAGATACATATCATCACAGACAGAGCGTGAAGTACGATTTGTAGGCCTTTCTACAGCTTTAGCAAATGCAGGGGATTTGGCTGATTGGTTGGGTGTTGGGGAAATCGGGCTCTTCAATTTTAAACCAAGCGTGAGACCTGTACCTCTTGAAGTTCATATCCAGGGATACCCTGGAAAGTTCTATTGCCCAAGGATGAATAGCATGAACAAACCAGCATATGCTGCGATATGCACCCACTCACCTACAAAACCAGTTTTAATATTTGTTTCCTCTCGTCGACAGACAAGGCTTACTGCATTGGACCTCATTCAGTTTGCAGCTTCAGATGAACATCCAAGGCAGTTTCTTAGCATGCCAGAAGACGACCTGCAGATGGTGCTTTCTCAGGTCACTGATAATAACCTTAGGCACACTTTGCAATTTGGCATTGGATTACATCACGCTGGTCTGAATGACAAGGATAGATCTCTAGTTGAAGAGCTTTTTGCAAACAACAAGATTCAGGTATTGGTTTGTACGAGCACATTGGCATGGGGTGTAAACCTTCCAGCCCATCTAGTCATCATTAAGGGAACAGAATATTATGATGGGAAAGCAAAAAGATATGTTGATTTTCCAATCACAGATATCTTGCAAATGATGGGTCGTGCTGGCCGGCCACAATTTGATCAGCATGGGAAAGCAGTCATTCTTGTTCATGAACCCAAAAAGAGCTTCTATAAAAAGTTCTTATATGAACCATTTCCCGTCGAGAGTAGTCTGAGGGAACAGTTGCATAATCACATCAACGCTGAGATAGTTTCTGGCACAATTTGCCATAGAGAGGATGCGCTCCATTATCTTACCTGGACATATTTATACCGTAGACTGATGTTTAATCCAGCTTACTATGGCTTGGACAATGCAGAAGCTGAAGTTGTAAATTCTTACTTGTCTAGACTAGTGCAGAACACATTTGAGGATCTCGAAGACAGTGGATGCATCGAGATGAATGATGACAGTGTGAAGCCAACGATGCTGGGTTCAATAGCATCCCAGTATTACCTTAGCTACATGACTGTATCAATGTTTGGTTCAAACATAAGTTCAGATACGTCGCTTGAAGTGTTCTTGCATATCCTATCGGCTGCTTCTGAATATAACGAACTTCCTGTGCGGCACAATGAGGAGAATTACAATGGAGCGTTGGCTGAGAGAGTTCGATATAAAGTGGATAAGGACCGGTTAGATGATCCACATGTCAAAGCCAATCTTCTGTTTCAGGCACATTTCTCGCAGTTAGAATTACCAATCAGTGATTACGTCACAGACTTGAAGTCGGTACTGGATCAAAGTATACGGGTCATCCAGGCCATGATAGATATATGTGCCAACAGTGGGTGGCTTTCAAGCTCAGTTACTTGCATGCATCTTCTGCAAATGGTCATGCAGGGTTTATGGTTTGATAAGGATTCTTCCTTGTGGATGATGCCATGCATGAATGTTGAACTTGCCGACTCACTAAGCAAAAGAGGAATATTCAGCGTGCAGCAACTACTGAATCTCCCCAAGACAACTTTGCAGACCATGATCGGAAACTTCCCGGTTTCAAAGTTTTATCAGGATCTGCAGCGTTTTCCCCACATCGAAATGAAGTTAAGAATTCAGGAAAAGGACAGCGGAAAGTCTCACTCTTTAAACATCAGATTGGTTAAGACCAATTACAGGCAAAACAAATCAAGAGCGTTTACCCCGCGATTTCCTAAGGTGAAAAACGAAGCGTGGTGGTTGGTCCTTGGGAACACCTCAACATCTGAATTATACGCTCTGAAGAGAGTATCCTTCTCCGATCACTTGGTTACTCACATGGAGTTACCAGCAGCTCCGAATAATATCCAGGGGATGAAATTGATTTTAGTTTCGGATTGTTATTTGGGCTTCGAGCAAGAGCACTCTATCTCAGAACTTATTCAACGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD Helicase_C Sec63

Protein Analysis

1878

Amino Acids

213.04

Weight (kDa)

6.33

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 231 - 405 1.1e-30 DEAD/DEAH box helicase
ResIII PF04851 238 - 404 3.3e-14 Type III restriction enzyme, res subunit
Helicase_C PF00271 526 - 608 3.7e-07 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 639 - 706 6.3e-15 Winged Helix-turn-helix domain
Sec63 PF02889 730 - 1037 4.6e-87 Sec63 Brl domain
DEAD PF00270 1079 - 1245 2.4e-27 DEAD/DEAH box helicase
ResIII PF04851 1087 - 1212 2.8e-11 Type III restriction enzyme, res subunit
Helicase_C PF00271 1357 - 1444 2.8e-09 Helicase conserved C-terminal domain
WHD_SNRNP200 PF23445 1475 - 1556 3.7e-31 Winged Helix-turn-helix domain
Sec63 PF02889 1561 - 1871 2.8e-66 Sec63 Brl domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 726, 1853, 2027
AarI CACCTGC 1 cut(s) 155
Acc36I ACCTGC 2 cut(s) 155, 4047
AccB7I CCANNNNNTGG 1 cut(s) 1760
AccI GTMKAC 3 cut(s) 3949, 4537, 5008
AccII CGCG 1 cut(s) 5407
AciI CCGC 9 cut(s) 706, 2120, 2527, 2983, 3515, 4828, 5331, 5405, 5629
AclI AACGTT 1 cut(s) 2911
AcoI YGGCCR 4 cut(s) 117, 3501, 4324, 4328
AcsI RAATTY 8 cut(s) 443, 653, 848, 1478, 1583, 2315, 4594, 5313
AcuI CTGAAG 4 cut(s) 873, 3978, 4605, 5501
AcyI GRCGYC 2 cut(s) 2266, 3436
AfiI CCNNNNNNNGG 9 cut(s) 126, 320, 1256, 1760, 2913, 4045, 4495, 5049, 5376
AflIII ACRYGT 1 cut(s) 4909
AgeI ACCGGT 1 cut(s) 4893
AhdI GACNNNNNGTC 2 cut(s) 3440, 4987
AhlI ACTAGT 3 cut(s) 745, 1135, 4612
AjuI GAANNNNNNNTTGG 8 cut(s) 1575, 1607, 2718, 2750, 3409, 3441, 4370, 4402
AleI CACNNNNGTG 1 cut(s) 1724
AloI GAACNNNNNNTCC 2 cut(s) 1581, 1613
Alw21I GWGCWC 5 cut(s) 535, 757, 3301, 4196, 5604
Alw26I GTCTC 9 cut(s) 1813, 1866, 2257, 2273, 3353, 3448, 3570, 3799, 5343
AlwNI CAGNNNCTG 6 cut(s) 350, 767, 1237, 2819, 4583, 5278
ApaI GGGCCC 1 cut(s) 1121
ApoI RAATTY 8 cut(s) 443, 653, 848, 1478, 1583, 2315, 4594, 5313
ArsI GACNNNNNNTTYG 2 cut(s) 4300, 4332
AseI ATTAAT 1 cut(s) 1352
AsiGI ACCGGT 1 cut(s) 4893
Asp700I GAANNNNTTC 3 cut(s) 2425, 4413, 5619
AspLEI GCGC 6 cut(s) 378, 419, 1155, 1644, 3087, 4504
AspS9I GGNCC 9 cut(s) 1117, 1118, 2402, 2619, 2756, 3641, 3973, 4891, 5443
AsuC2I CCSGG 1 cut(s) 5255
AvaII GGWCC 5 cut(s) 2402, 3641, 3973, 4891, 5443
AxyI CCTNAGG 2 cut(s) 4076, 5415
BaeGI GKGCMC 2 cut(s) 1121, 1296
BaeI ACNNNNGTAYC 2 cut(s) 1385, 1418
BalI TGGCCA 2 cut(s) 119, 3503
BamHI GGATCC 1 cut(s) 2802
BanII GRGCYC 3 cut(s) 1121, 3301, 3783
BarI GAAGNNNNNNTAC 2 cut(s) 3365, 3397
BauI CACGAG 2 cut(s) 1067, 2454
BbsI GAAGAC 2 cut(s) 4038, 4647
Bbv12I GWGCWC 5 cut(s) 535, 757, 3301, 4196, 5604
BceAI ACGGC 1 cut(s) 371
BcgI CGANNNNNNTGC 4 cut(s) 2361, 2395, 4926, 4960
BciVI GTATCC 3 cut(s) 671, 3830, 5500
BclI TGATCA 2 cut(s) 3603, 4339
BcnI CCSGG 1 cut(s) 5255
BcoDI GTCTC 9 cut(s) 1813, 1866, 2257, 2273, 3353, 3448, 3570, 3799, 5343
BcuI ACTAGT 3 cut(s) 745, 1135, 4612
BfmI CTRYAG 4 cut(s) 1911, 3723, 4040, 5279
BfoI RGCGCY 2 cut(s) 420, 1645
BfuAI ACCTGC 2 cut(s) 155, 4047
BfuI GTATCC 3 cut(s) 671, 3830, 5500
BglII AGATCT 5 cut(s) 178, 1564, 1800, 2108, 4136
BmcAI AGTACT 1 cut(s) 85
Bme18I GGWCC 5 cut(s) 2402, 3641, 3973, 4891, 5443
BmeRI GACNNNNNGTC 2 cut(s) 3440, 4987
BmgT120I GGNCC 9 cut(s) 1117, 1118, 2402, 2619, 2756, 3641, 3973, 4891, 5443
BmiI GGNNCC 8 cut(s) 547, 1118, 1119, 2403, 2621, 2804, 3000, 3643
BmrI ACTGGG 2 cut(s) 275, 4702
BmuI ACTGGG 2 cut(s) 275, 4702
BpiI GAAGAC 2 cut(s) 4038, 4647
BpmI CTGGAG 3 cut(s) 120, 2781, 3474
Bpu10I CCTNAGC 2 cut(s) 2991, 4718
BpuEI CTTGAG 7 cut(s) 61, 525, 645, 2113, 2218, 2378, 3671
BpuMI CCSGG 1 cut(s) 5255
Bsa29I ATCGAT 2 cut(s) 6, 2466
BsaAI YACGTR 1 cut(s) 308
BsaBI GATNNNNATC 4 cut(s) 426, 682, 3679, 4002
BsaHI GRCGYC 2 cut(s) 2266, 3436
BsaI GGTCTC 2 cut(s) 1813, 3799
BsaWI WCCGGW 1 cut(s) 4893
BsaXI ACNNNNNCTCC 4 cut(s) 1581, 1611, 5199, 5229
Bsc4I CCNNNNNNNGG 9 cut(s) 126, 320, 1256, 1760, 2913, 4045, 4495, 5049, 5376
Bse118I RCCGGY 2 cut(s) 4326, 4893
Bse21I CCTNAGG 2 cut(s) 4076, 5415
Bse3DI GCAATG 1 cut(s) 1416
Bse8I GATNNNNATC 4 cut(s) 426, 682, 3679, 4002
BseCI ATCGAT 2 cut(s) 6, 2466
BseJI GATNNNNATC 4 cut(s) 426, 682, 3679, 4002
BseLI CCNNNNNNNGG 9 cut(s) 126, 320, 1256, 1760, 2913, 4045, 4495, 5049, 5376
BseMI GCAATG 1 cut(s) 1416
BseRI GAGGAG 2 cut(s) 2299, 4853
BseSI GKGCMC 2 cut(s) 1121, 1296
BseYI CCCAGC 4 cut(s) 1607, 1814, 3360, 4690
BsgI GTGCAG 3 cut(s) 2112, 4637, 5219
Bsh1236I CGCG 1 cut(s) 5407
BshTI ACCGGT 1 cut(s) 4893
BshVI ATCGAT 2 cut(s) 6, 2466
BsiHKAI GWGCWC 5 cut(s) 535, 757, 3301, 4196, 5604
BsiSI CCGG 3 cut(s) 4327, 4894, 5255
BslFI GGGAC 2 cut(s) 282, 1750
BslI CCNNNNNNNGG 9 cut(s) 126, 320, 1256, 1760, 2913, 4045, 4495, 5049, 5376
BsmAI GTCTC 9 cut(s) 1813, 1866, 2257, 2273, 3353, 3448, 3570, 3799, 5343
BsmBI CGTCTC 4 cut(s) 1866, 2257, 3353, 3448
BsmFI GGGAC 2 cut(s) 282, 1750
Bso31I GGTCTC 2 cut(s) 1813, 3799
Bsp120I GGGCCC 1 cut(s) 1117
Bsp1286I GDGCHC 8 cut(s) 535, 757, 1121, 1296, 3301, 3783, 4196, 5604
Bsp1407I TGTACA 4 cut(s) 1679, 2189, 2445, 3216
Bsp19I CCATGG 1 cut(s) 2798
BspACI CCGC 9 cut(s) 706, 2120, 2527, 2983, 3515, 4828, 5331, 5405, 5629
BspDI ATCGAT 2 cut(s) 6, 2466
BspFNI CGCG 1 cut(s) 5407
BspHI TCATGA 1 cut(s) 4369
BspLI GGNNCC 8 cut(s) 547, 1118, 1119, 2403, 2621, 2804, 3000, 3643
BspMAI CTGCAG 2 cut(s) 4044, 5283
BspMI ACCTGC 2 cut(s) 155, 4047
BspQI GCTCTTC 3 cut(s) 540, 3788, 4143
BspTNI GGTCTC 2 cut(s) 1813, 3799
BsrDI GCAATG 1 cut(s) 1416
BsrFI RCCGGY 2 cut(s) 4326, 4893
BsrGI TGTACA 4 cut(s) 1679, 2189, 2445, 3216
BssAI RCCGGY 2 cut(s) 4326, 4893
BssNAI GTATAC 1 cut(s) 5009
BssNI GRCGYC 2 cut(s) 2266, 3436
BssSI CACGAG 2 cut(s) 1067, 2454
BssT1I CCWWGG 5 cut(s) 2478, 2798, 3860, 4007, 5446
Bst1107I GTATAC 1 cut(s) 5009
Bst2BI CACGAG 2 cut(s) 1067, 2454
Bst6I CTCTTC 6 cut(s) 540, 1268, 3356, 3788, 4143, 5477
BstACI GRCGYC 2 cut(s) 2266, 3436
BstAPI GCANNNNNTGC 7 cut(s) 530, 1139, 3893, 4048, 4087, 4485, 5086
BstAUI TGTACA 4 cut(s) 1679, 2189, 2445, 3216
BstBAI YACGTR 1 cut(s) 308
BstDSI CCRYGG 2 cut(s) 2798, 3637
BstFNI CGCG 1 cut(s) 5407
BstH2I RGCGCY 2 cut(s) 420, 1645
BstHHI GCGC 6 cut(s) 378, 419, 1155, 1644, 3087, 4504
BstMAI GTCTC 9 cut(s) 1813, 1866, 2257, 2273, 3353, 3448, 3570, 3799, 5343
BstNSI RCATGY 6 cut(s) 63, 964, 1786, 4027, 4913, 5080
BstSFI CTRYAG 4 cut(s) 1911, 3723, 4040, 5279
BstSLI GKGCMC 2 cut(s) 1121, 1296
BstUI CGCG 1 cut(s) 5407
BstV2I GAAGAC 2 cut(s) 4038, 4647
BstXI CCANNNNNNTGG 1 cut(s) 1219
BstZ17I GTATAC 1 cut(s) 5009
Bsu15I ATCGAT 2 cut(s) 6, 2466
Bsu36I CCTNAGG 2 cut(s) 4076, 5415
BsuI GTATCC 3 cut(s) 671, 3830, 5500
BsuTUI ATCGAT 2 cut(s) 6, 2466
BtgI CCRYGG 2 cut(s) 2798, 3637
BtgZI GCGATG 1 cut(s) 899
BtsI GCAGTG 2 cut(s) 1051, 3125
BtsIMutI CAGTG 9 cut(s) 1051, 1719, 3125, 3303, 4062, 4651, 4678, 4972, 5053
BveI ACCTGC 2 cut(s) 155, 4047
CaiI CAGNNNCTG 6 cut(s) 350, 767, 1237, 2819, 4583, 5278
CciI TCATGA 1 cut(s) 4369
CfoI GCGC 6 cut(s) 378, 419, 1155, 1644, 3087, 4504
Cfr10I RCCGGY 2 cut(s) 4326, 4893
Cfr13I GGNCC 9 cut(s) 1117, 1118, 2402, 2619, 2756, 3641, 3973, 4891, 5443
ClaI ATCGAT 2 cut(s) 6, 2466
CseI GACGC 2 cut(s) 2274, 3444
CspAI ACCGGT 1 cut(s) 4893
CspCI CAANNNNNGTGG 2 cut(s) 2897, 2932
DraI TTTAAA 5 cut(s) 853, 1950, 2847, 3793, 5349
DriI GACNNNNNGTC 2 cut(s) 3440, 4987
EaeI YGGCCR 4 cut(s) 117, 3501, 4324, 4328
Eam1104I CTCTTC 6 cut(s) 540, 1268, 3356, 3788, 4143, 5477
Eam1105I GACNNNNNGTC 2 cut(s) 3440, 4987
EarI CTCTTC 6 cut(s) 540, 1268, 3356, 3788, 4143, 5477
Ecl136II GAGCTC 1 cut(s) 3299
Eco130I CCWWGG 5 cut(s) 2478, 2798, 3860, 4007, 5446
Eco147I AGGCCT 1 cut(s) 3717
Eco24I GRGCYC 3 cut(s) 1121, 3301, 3783
Eco31I GGTCTC 2 cut(s) 1813, 3799
Eco32I GATATC 2 cut(s) 3520, 4300
Eco47I GGWCC 5 cut(s) 2402, 3641, 3973, 4891, 5443
Eco53kI GAGCTC 1 cut(s) 3299
Eco57I CTGAAG 4 cut(s) 873, 3978, 4605, 5501
Eco81I CCTNAGG 2 cut(s) 4076, 5415
EcoICRI GAGCTC 1 cut(s) 3299
EcoO109I RGGNCCY 3 cut(s) 1117, 1118, 2402
EcoRI GAATTC 1 cut(s) 5313
EcoRV GATATC 2 cut(s) 3520, 4300
EcoT14I CCWWGG 5 cut(s) 2478, 2798, 3860, 4007, 5446
EcoT22I ATGCAT 4 cut(s) 61, 4655, 5082, 5150
EcoT38I GRGCYC 3 cut(s) 1121, 3301, 3783
ErhI CCWWGG 5 cut(s) 2478, 2798, 3860, 4007, 5446
Esp3I CGTCTC 4 cut(s) 1866, 2257, 3353, 3448
FalI AAGNNNNNCTT 4 cut(s) 2840, 2872, 4908, 4940
FaqI GGGAC 2 cut(s) 282, 1750
FauI CCCGC 2 cut(s) 2520, 5412
FauNDI CATATG 2 cut(s) 2879, 3889
FbaI TGATCA 2 cut(s) 3603, 4339
FblI GTMKAC 3 cut(s) 3949, 4537, 5008
FriOI GRGCYC 3 cut(s) 1121, 3301, 3783
FseI GGCCGGCC 1 cut(s) 4330
GlaI GCGC 6 cut(s) 377, 418, 1154, 1643, 3086, 4503
GsaI CCCAGC 4 cut(s) 1611, 1818, 3364, 4694
GsuI CTGGAG 3 cut(s) 120, 2781, 3474
HaeII RGCGCY 2 cut(s) 420, 1645
HapII CCGG 3 cut(s) 4327, 4894, 5255
HgaI GACGC 2 cut(s) 2274, 3444
HhaI GCGC 6 cut(s) 378, 419, 1155, 1644, 3087, 4504
Hin1I GRCGYC 2 cut(s) 2266, 3436
Hin6I GCGC 6 cut(s) 376, 417, 1153, 1642, 3085, 4502
HinP1I GCGC 6 cut(s) 376, 417, 1153, 1642, 3085, 4502
HincII GTYRAC 2 cut(s) 1904, 3950
HindII GTYRAC 2 cut(s) 1904, 3950
HindIII AAGCTT 6 cut(s) 31, 1073, 1992, 2509, 2678, 3008
HpaII CCGG 3 cut(s) 4327, 4894, 5255
Hpy166II GTNNAC 9 cut(s) 1904, 2053, 2854, 3641, 3950, 4213, 4538, 5009, 5400
Hpy8I GTNNAC 9 cut(s) 1904, 2053, 2854, 3641, 3950, 4213, 4538, 5009, 5400
Hpy99I CGWCG 4 cut(s) 245, 3951, 4424, 4771
HpyCH4IV ACGT 7 cut(s) 307, 1859, 2131, 2246, 2911, 4766, 4974
HpySE526I ACGT 7 cut(s) 307, 1859, 2131, 2246, 2911, 4766, 4974
Hsp92I GRCGYC 2 cut(s) 2266, 3436
HspAI GCGC 6 cut(s) 376, 417, 1153, 1642, 3085, 4502
KroI GCCGGC 1 cut(s) 4326
KroNI GCCGGC 1 cut(s) 4328
Ksp22I TGATCA 2 cut(s) 3603, 4339
LguI GCTCTTC 3 cut(s) 540, 3788, 4143
LmnI GCTCC 9 cut(s) 760, 764, 2738, 3296, 3304, 3629, 4509, 4853, 5539
MaeII ACGT 7 cut(s) 307, 1859, 2131, 2246, 2911, 4766, 4974
MfeI CAATTG 1 cut(s) 1742
MhlI GDGCHC 8 cut(s) 535, 757, 1121, 1296, 3301, 3783, 4196, 5604
MlsI TGGCCA 2 cut(s) 119, 3503
MluNI TGGCCA 2 cut(s) 119, 3503
MlyI GAGTC 5 cut(s) 131, 1254, 1732, 2833, 5162
MmeI TCCRAC 3 cut(s) 782, 1573, 2565
Mox20I TGGCCA 2 cut(s) 119, 3503
Mph1103I ATGCAT 4 cut(s) 61, 4655, 5082, 5150
MroNI GCCGGC 1 cut(s) 4326
MroXI GAANNNNTTC 3 cut(s) 2425, 4413, 5619
MscI TGGCCA 2 cut(s) 119, 3503
MslI CAYNNNNRTG 7 cut(s) 785, 843, 1724, 2274, 4201, 4658, 5300
Msp20I TGGCCA 2 cut(s) 119, 3503
MspA1I CMGCKG 2 cut(s) 3515, 5331
MspI CCGG 3 cut(s) 4327, 4894, 5255
MunI CAATTG 1 cut(s) 1742
MvnI CGCG 1 cut(s) 5407
NaeI GCCGGC 1 cut(s) 4328
NciI CCSGG 1 cut(s) 5255
NcoI CCATGG 1 cut(s) 2798
NdeI CATATG 2 cut(s) 2879, 3889
NgoMIV GCCGGC 1 cut(s) 4326
NlaIV GGNNCC 8 cut(s) 547, 1118, 1119, 2403, 2621, 2804, 3000, 3643
NmeAIII GCCGAG 1 cut(s) 3355
NmuCI GTSAC 7 cut(s) 167, 895, 1046, 1975, 3181, 4060, 4975
NsiI ATGCAT 4 cut(s) 61, 4655, 5082, 5150
NspI RCATGY 6 cut(s) 63, 964, 1786, 4027, 4913, 5080
OliI CACNNNNGTG 1 cut(s) 1724
PaeI GCATGC 3 cut(s) 1786, 4027, 5080
PagI TCATGA 1 cut(s) 4369
PaqCI CACCTGC 1 cut(s) 155
PceI AGGCCT 1 cut(s) 3717
PciI ACATGT 1 cut(s) 4909
PciSI GCTCTTC 3 cut(s) 540, 3788, 4143
PdiI GCCGGC 1 cut(s) 4328
PdmI GAANNNNTTC 3 cut(s) 2425, 4413, 5619
PflMI CCANNNNNTGG 1 cut(s) 1760
PinAI ACCGGT 1 cut(s) 4893
PleI GAGTC 5 cut(s) 131, 1254, 1732, 2832, 5162
PpsI GAGTC 5 cut(s) 131, 1254, 1732, 2832, 5162
Ppu21I YACGTR 1 cut(s) 308
PpuMI RGGWCCY 1 cut(s) 2402
PscI ACATGT 1 cut(s) 4909
PshBI ATTAAT 1 cut(s) 1352
PsiI TTATAA 3 cut(s) 726, 1853, 2027
Psp124BI GAGCTC 1 cut(s) 3301
Psp1406I AACGTT 1 cut(s) 2911
Psp5II RGGWCCY 1 cut(s) 2402
PspFI CCCAGC 4 cut(s) 1607, 1814, 3360, 4690
PspN4I GGNNCC 8 cut(s) 547, 1118, 1119, 2403, 2621, 2804, 3000, 3643
PspOMI GGGCCC 1 cut(s) 1117
PspPI GGNCC 9 cut(s) 1117, 1118, 2402, 2619, 2756, 3641, 3973, 4891, 5443
PspPPI RGGWCCY 1 cut(s) 2402
PstI CTGCAG 2 cut(s) 4044, 5283
PstNI CAGNNNCTG 6 cut(s) 350, 767, 1237, 2819, 4583, 5278
RigI GGCCGGCC 1 cut(s) 4330
RseI CAYNNNNRTG 7 cut(s) 785, 843, 1724, 2274, 4201, 4658, 5300
SacI GAGCTC 1 cut(s) 3301
SalI GTCGAC 1 cut(s) 3948
SapI GCTCTTC 3 cut(s) 540, 3788, 4143
Sau96I GGNCC 9 cut(s) 1117, 1118, 2402, 2619, 2756, 3641, 3973, 4891, 5443
ScaI AGTACT 1 cut(s) 85
SchI GAGTC 5 cut(s) 131, 1254, 1732, 2833, 5162
SduI GDGCHC 8 cut(s) 535, 757, 1121, 1296, 3301, 3783, 4196, 5604
SfcI CTRYAG 4 cut(s) 1911, 3723, 4040, 5279
SinI GGWCC 5 cut(s) 2402, 3641, 3973, 4891, 5443
SmiI ATTTAAAT 1 cut(s) 1950
SmiMI CAYNNNNRTG 7 cut(s) 785, 843, 1724, 2274, 4201, 4658, 5300
SmlI CTYRAG 7 cut(s) 40, 540, 660, 2092, 2233, 2357, 3650
SmoI CTYRAG 7 cut(s) 40, 540, 660, 2092, 2233, 2357, 3650
SpeI ACTAGT 3 cut(s) 745, 1135, 4612
SphI GCATGC 3 cut(s) 1786, 4027, 5080
SseBI AGGCCT 1 cut(s) 3717
SsiI CCGC 9 cut(s) 706, 2120, 2527, 2983, 3515, 4828, 5331, 5405, 5629
SspI AATATT 6 cut(s) 2326, 2783, 3030, 3933, 4253, 5190
SstI GAGCTC 1 cut(s) 3301
StuI AGGCCT 1 cut(s) 3717
StyI CCWWGG 5 cut(s) 2478, 2798, 3860, 4007, 5446
SwaI ATTTAAAT 1 cut(s) 1950
TaiI ACGT 7 cut(s) 310, 1862, 2134, 2249, 2914, 4769, 4977
TatI WGTACW 6 cut(s) 83, 457, 1679, 2189, 2445, 3216
TauI GCSGC 2 cut(s) 708, 4831
TscAI CASTG 9 cut(s) 1051, 1726, 3132, 3310, 4069, 4651, 4678, 4972, 5053
TseFI GTSAC 7 cut(s) 167, 895, 1046, 1975, 3181, 4060, 4975
Tsp45I GTSAC 7 cut(s) 167, 895, 1046, 1975, 3181, 4060, 4975
TspGWI ACGGA 1 cut(s) 3050
TspRI CASTG 9 cut(s) 1051, 1726, 3132, 3310, 4069, 4651, 4678, 4972, 5053
Van91I CCANNNNNTGG 1 cut(s) 1760
VpaK11BI GGWCC 5 cut(s) 2402, 3641, 3973, 4891, 5443
VspI ATTAAT 1 cut(s) 1352
XapI RAATTY 8 cut(s) 443, 653, 848, 1478, 1583, 2315, 4594, 5313
XbaI TCTAGA 1 cut(s) 4607
XceI RCATGY 6 cut(s) 63, 964, 1786, 4027, 4913, 5080
XcmI CCANNNNNNNNNTGG 2 cut(s) 2877, 3839
XmiI GTMKAC 3 cut(s) 3949, 4537, 5008
XmnI GAANNNNTTC 3 cut(s) 2425, 4413, 5619
ZrmI AGTACT 1 cut(s) 85
Zsp2I ATGCAT 4 cut(s) 61, 4655, 5082, 5150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.