pycom07g06570

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0014794
Physical Location & Seq
Reverse (-)
5869217 .. 5870668
1452 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06570.5

Sequence Viewer

Length: 924 bp
ATGGTAGGTAGCATAGAAGTACATGTAAAGCAATCCCCTCAACATGTAGAAGTGAATCTCTCTGAACTGAAGGGCTACGAGAAGCATGTCATTGTGGAACTCATGAGAGAGACACAGGACAATATGCTGAACAAGTCTCTGCCATGCAGCCTTGACAACTGCCGAGGTATATATATGTGGATTAAAAACACAATAATCTTATACGAGGCGGACAAGCTGTCGACCGATGCCCTACTGTATATAAAGTGGCTTTTAGAAAGGTATAAAGGGTGTAATAAGGTCTTCTTCTGCTGCTCTGATATCTCAAAGCTTCAGGCAATCAAATCGCTTTGCACCATCGTTGAGCTTTTACCTCCTTCCAAGAATGAGATTGTTGAAGTTTTAAAATTCATATCTAAAAAAGAAGGCATAGATTTGCCAGATGAACTGGCTGAAAGGTTTGCCCAAAATTCGAGTAATATTCTCCGTCAAGCGATTCGTTCATTCGAGGCTACTTGGAAGAAAAGCTATCCTTTTAAAGAAGATCAAGTAATTCTGACAGGATGGGAAGATAACATTGCAGATATTGCCAAGAATATGATTGAAGAGCAAAGCCCAAAACAGTATGCTCCTTTGAACAAATTAATATGTAGTAAGACTACACTATACAATATATGTACCACGGAACACTATACAATATATGTACCACGGAAAACCATGTTCGCCTTGTTCTCAAATTTCCAAGGTATATATTATTGTATGAGCCGGTTTTATATAACCTGCCCCTCACAGCTATATATAGTCCGCGGAAAGCTTCAAAATCTTATAGTTCATTCCGTTTGTCCCGAATTCATTTTCAAATCCCTAGTAGCACAGCTGAAGAAGTATGTGGACGACAGCCTGCAGGATCGAGTTGAGAGGATGTACGAAGAATACAACGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

35.81

Weight (kDa)

7.47

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014794)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08091
malus_domestica MD02G1230000.v1.1 MD07G1082400.v1.1
prunus_persica Prupe.2G108000_v2.0.a1
pyrus_communis pycom07g06570 pycom07g06580
rosa_chinensis RchiOBHm_Chr1g0341191
rosa_laevigata RLG00000029069
rosa_multiflora Rmu_sc0004092.1_g000026 Rmu_sc0010892.1_g000010
rosa_roxburghii Rroxscaffold_4G00311790
rosa_rugosa Rorug01G0159600.1
rosa_samantha Rh1AG173900 Rh1BG141800 Rh1CG161700 Rh1DG173800
rosa_wichuraiana Rw1G014440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 767
AccI GTMKAC 1 cut(s) 221
AccII CGCG 1 cut(s) 786
AciI CCGC 3 cut(s) 209, 784, 786
AclWI GGATC 1 cut(s) 894
AcsI RAATTY 4 cut(s) 386, 448, 715, 827
AcuI CTGAAG 3 cut(s) 89, 296, 878
AfaI GTAC 4 cut(s) 21, 658, 684, 905
AflIII ACRYGT 2 cut(s) 22, 43
AgsI TTSAA 5 cut(s) 377, 584, 616, 797, 838
AhdI GACNNNNNGTC 1 cut(s) 217
AluBI AGCT 7 cut(s) 217, 310, 346, 507, 772, 793, 856
AluI AGCT 7 cut(s) 217, 310, 346, 507, 772, 793, 856
Alw26I GTCTC 2 cut(s) 104, 141
AlwI GGATC 1 cut(s) 894
ApeKI GCWGC 2 cut(s) 147, 291
ApoI RAATTY 4 cut(s) 386, 448, 715, 827
AseI ATTAAT 1 cut(s) 623
BbsI GAAGAC 1 cut(s) 274
BbvI GCAGC 2 cut(s) 159, 278
BccI CCATC 2 cut(s) 344, 537
BcgI CGANNNNNNTGC 2 cut(s) 306, 340
BcoDI GTCTC 2 cut(s) 104, 141
BfaI CTAG 1 cut(s) 845
BfmI CTRYAG 1 cut(s) 881
BfuAI ACCTGC 1 cut(s) 767
BisI GCNGC 2 cut(s) 148, 292
BlsI GCNGC 2 cut(s) 149, 293
BmeRI GACNNNNNGTC 1 cut(s) 217
BmsI GCATC 1 cut(s) 217
BpiI GAAGAC 1 cut(s) 274
BsaJI CCNNGG 5 cut(s) 163, 660, 686, 721, 784
Bse118I RCCGGY 1 cut(s) 744
Bse1I ACTGG 1 cut(s) 432
Bse3DI GCAATG 1 cut(s) 555
BseDI CCNNGG 5 cut(s) 163, 660, 686, 721, 784
BseGI GGATG 2 cut(s) 548, 906
BseMI GCAATG 1 cut(s) 555
BseNI ACTGG 1 cut(s) 432
BseXI GCAGC 2 cut(s) 159, 278
Bsh1236I CGCG 1 cut(s) 786
Bsh1285I CGRYCG 1 cut(s) 225
BsiEI CGRYCG 1 cut(s) 225
BsiSI CCGG 1 cut(s) 745
BslFI GGGAC 1 cut(s) 807
BsmAI GTCTC 2 cut(s) 104, 141
BsmFI GGGAC 1 cut(s) 807
Bsp143I GATC 2 cut(s) 523, 886
BspACI CCGC 3 cut(s) 209, 784, 786
BspFNI CGCG 1 cut(s) 786
BspHI TCATGA 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 885
BspMI ACCTGC 1 cut(s) 767
BspPI GGATC 1 cut(s) 894
BspQI GCTCTTC 1 cut(s) 579
BsrDI GCAATG 1 cut(s) 555
BsrFI RCCGGY 1 cut(s) 744
BsrI ACTGG 1 cut(s) 432
BssAI RCCGGY 1 cut(s) 744
BssECI CCNNGG 5 cut(s) 163, 660, 686, 721, 784
BssMI GATC 2 cut(s) 523, 886
BssT1I CCWWGG 1 cut(s) 721
Bst4CI ACNGT 2 cut(s) 237, 603
Bst6I CTCTTC 1 cut(s) 579
BstAPI GCANNNNNTGC 1 cut(s) 566
BstC8I GCNNGC 1 cut(s) 881
BstDSI CCRYGG 3 cut(s) 660, 686, 784
BstF5I GGATG 2 cut(s) 548, 906
BstFNI CGCG 1 cut(s) 786
BstKTI GATC 2 cut(s) 526, 889
BstMAI GTCTC 2 cut(s) 104, 141
BstMBI GATC 2 cut(s) 523, 886
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 1 cut(s) 566
BstNSI RCATGY 3 cut(s) 26, 47, 89
BstSFI CTRYAG 1 cut(s) 881
BstUI CGCG 1 cut(s) 786
BstV1I GCAGC 2 cut(s) 159, 278
BstV2I GAAGAC 1 cut(s) 274
BtgI CCRYGG 3 cut(s) 660, 686, 784
BtsCI GGATG 2 cut(s) 548, 906
BveI ACCTGC 1 cut(s) 767
Cac8I GCNNGC 1 cut(s) 881
CciI TCATGA 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 744
Cfr42I CCGCGG 1 cut(s) 787
Csp6I GTAC 4 cut(s) 20, 657, 683, 904
CviAII CATG 6 cut(s) 23, 44, 86, 103, 144, 697
CviQI GTAC 4 cut(s) 20, 657, 683, 904
DpnI GATC 2 cut(s) 525, 888
DpnII GATC 2 cut(s) 523, 886
DraI TTTAAA 2 cut(s) 384, 517
DriI GACNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 579
Eam1105I GACNNNNNGTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 579
EciI GGCGGA 1 cut(s) 224
Eco130I CCWWGG 1 cut(s) 721
Eco32I GATATC 1 cut(s) 301
Eco57I CTGAAG 3 cut(s) 89, 296, 878
EcoRI GAATTC 1 cut(s) 827
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 721
ErhI CCWWGG 1 cut(s) 721
FaeI CATG 6 cut(s) 26, 47, 89, 106, 147, 700
FalI AAGNNNNNCTT 4 cut(s) 269, 301, 496, 528
FaqI GGGAC 1 cut(s) 807
FatI CATG 6 cut(s) 22, 43, 85, 102, 143, 696
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 2 cut(s) 148, 292
FokI GGATG 2 cut(s) 555, 913
Fsp4HI GCNGC 2 cut(s) 148, 292
FspBI CTAG 1 cut(s) 845
GluI GCNGC 2 cut(s) 148, 292
HapII CCGG 1 cut(s) 745
Hin1II CATG 6 cut(s) 26, 47, 89, 106, 147, 700
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HindIII AAGCTT 2 cut(s) 308, 791
HinfI GANTC 2 cut(s) 55, 475
HpaII CCGG 1 cut(s) 745
Hpy166II GTNNAC 2 cut(s) 222, 871
Hpy188I TCNGA 3 cut(s) 64, 298, 537
Hpy188III TCNNGA 2 cut(s) 103, 824
Hpy8I GTNNAC 2 cut(s) 222, 871
HpyAV CCTTC 3 cut(s) 64, 366, 398
HpyCH4III ACNGT 2 cut(s) 237, 603
HpyCH4IV ACGT 1 cut(s) 918
HpyCH4V TGCA 4 cut(s) 147, 333, 560, 883
HpyF10VI GCNNNNNNNGC 1 cut(s) 566
HpySE526I ACGT 1 cut(s) 918
Hsp92II CATG 6 cut(s) 26, 47, 89, 106, 147, 700
KspI CCGCGG 1 cut(s) 787
Kzo9I GATC 2 cut(s) 523, 886
LguI GCTCTTC 1 cut(s) 579
LmnI GCTCC 1 cut(s) 613
LpnPI CCDG 9 cut(s) 101, 299, 413, 432, 525, 758, 772, 869, 893
Lsp1109I GCAGC 2 cut(s) 159, 278
LweI GCATC 1 cut(s) 217
MaeI CTAG 1 cut(s) 845
MaeII ACGT 1 cut(s) 918
MalI GATC 2 cut(s) 525, 888
MboI GATC 2 cut(s) 523, 886
MboII GAAGA 8 cut(s) 274, 277, 511, 533, 560, 596, 871, 920
MluCI AATT 6 cut(s) 386, 448, 531, 620, 715, 827
MnlI CCTC 7 cut(s) 48, 158, 199, 363, 481, 775, 891
MseI TTAA 4 cut(s) 183, 383, 516, 623
MspA1I CMGCKG 2 cut(s) 786, 856
MspI CCGG 1 cut(s) 745
MvnI CGCG 1 cut(s) 786
MwoI GCNNNNNNNGC 1 cut(s) 566
NdeII GATC 2 cut(s) 523, 886
NlaIII CATG 6 cut(s) 26, 47, 89, 106, 147, 700
NmeAIII GCCGAG 1 cut(s) 188
NspI RCATGY 3 cut(s) 26, 47, 89
PagI TCATGA 1 cut(s) 102
PciI ACATGT 2 cut(s) 22, 43
PciSI GCTCTTC 1 cut(s) 579
PfeI GAWTC 2 cut(s) 55, 475
PkrI GCNGC 2 cut(s) 149, 293
PscI ACATGT 2 cut(s) 22, 43
PshBI ATTAAT 1 cut(s) 623
PstI CTGCAG 1 cut(s) 885
PvuII CAGCTG 1 cut(s) 856
RsaI GTAC 4 cut(s) 21, 658, 684, 905
RsaNI GTAC 4 cut(s) 20, 657, 683, 904
SacII CCGCGG 1 cut(s) 787
SalI GTCGAC 1 cut(s) 220
SapI GCTCTTC 1 cut(s) 579
SaqAI TTAA 4 cut(s) 183, 383, 516, 623
SatI GCNGC 2 cut(s) 148, 292
Sau3AI GATC 2 cut(s) 523, 886
SbfI CCTGCAGG 1 cut(s) 885
SdaI CCTGCAGG 1 cut(s) 885
SfaNI GCATC 1 cut(s) 217
SfcI CTRYAG 1 cut(s) 881
Sfr303I CCGCGG 1 cut(s) 787
SgrBI CCGCGG 1 cut(s) 787
Sse8387I CCTGCAGG 1 cut(s) 885
Sse9I AATT 6 cut(s) 386, 448, 531, 620, 715, 827
SsiI CCGC 3 cut(s) 209, 784, 786
SspI AATATT 1 cut(s) 460
SspMI CTAG 1 cut(s) 845
StyI CCWWGG 1 cut(s) 721
TaaI ACNGT 2 cut(s) 237, 603
TaiI ACGT 1 cut(s) 921
TaqI TCGA 4 cut(s) 221, 452, 486, 889
TaqII GACCGA 1 cut(s) 239
TasI AATT 6 cut(s) 386, 448, 531, 620, 715, 827
TatI WGTACW 1 cut(s) 19
TfiI GAWTC 2 cut(s) 55, 475
Tru1I TTAA 4 cut(s) 183, 383, 516, 623
Tru9I TTAA 4 cut(s) 183, 383, 516, 623
TseI GCWGC 2 cut(s) 147, 291
TspDTI ATGAA 5 cut(s) 379, 438, 471, 800, 820
TspGWI ACGGA 4 cut(s) 455, 677, 703, 805
VspI ATTAAT 1 cut(s) 623
XapI RAATTY 4 cut(s) 386, 448, 715, 827
XceI RCATGY 3 cut(s) 26, 47, 89
XmiI GTMKAC 1 cut(s) 221
XspI CTAG 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.