pycom07g06580

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0014794
Physical Location & Seq
Reverse (-)
5872397 .. 5873626
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06580.2

Sequence Viewer

Length: 1140 bp
ATGTCATCCGACACAAAACAAATGAAGTTGCAGACGCAACTCTGCAGCACAATGCCAAGCCCTTCAATAATCTCCCGCTCTTCGTCCTACCCGCAGTTCACAAAGACATGGTCTTCGCTGTACACTCTCCCCGAAAACCTCCCTCCGGCCAACAACTCTGCATGCTCAGCTCCACCATCGAAGTTCACTTCTGCCAAGCTCTCCTTGGGCACGAAGTTCAGAAAAATCATCAGGCGGAGGGGGAATTCGAACTTGACAGAGCAAAGCCTCCAGGTTCACAATAACGCGCACGTTGAGGGAGATGTGATCAAGCTTCACAAGAGAGTAGAGCAAGTTAGTCTTCAAGACTATTATAAAGCTGAGCAGGAGAGAAGCTCAAAGTATAGTCCCTATTACATTAGGGGACTCACGTACCCAGCCTTCGTTGTCCCTAAGCATCCAAGTAATACTCCCGGGCGCGAAAGCCAGACTTCTCATCGTTCTTGGAACTCATTCTCCACCTTCTTTTCGGGGTTCAAAATGCGAGAGTGGAGCTCTTGCATTGGTTCGAAAAGAGAGGATTACAACAAACTTAGGCCGGAAATGTACACTGCGATAAAACCCCCCAAACCACCTGTAATTAACTCCTCATCATCGACAGTTACAGAGATTTCTTTGGTGGAGAGCAAAGAGAGGAAGGAGGTTGGGAGGAGTAGCAATGAATCTAACAAAAAGAAGCCCTTGAGAGAGAGAGTGAGAACAGGAGCAGCAACAGCTGCTGCTTCGGCTACGCCGAGAAATGATCAAATTAAGGTGACGAGTGTCGCTGAAAAGGAGTATGCGTGGGCAGACAAGTACCAGCCTCTGTGCTTAGAAGATTTCATATGCAATAGAGACAAAGCAATTCAACTGCAGGCTTTGGCCAAACAAGGAGGATGTGGACATTTCATATTTGAAGGACCGCCGGGAGTGGGGAAGAGAACGATGATCTGGGCTATGCTTCGAGAGGCTTTTGGACGCGACACAATAAATGTATGTAGACATGATTCACTATATTTCTCATTGCTTATTTGTAGCTTTTATTCTTCTCAATCTTTTAATGCTTCTTTGAAATATATTATCAGGCCACGGAAGAGTCCAAGGCATTCAACCTCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
No domains found.

Protein Analysis

380

Amino Acids

42.88

Weight (kDa)

9.86

Isoelectric Point (pI)

60.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014794)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08091
malus_domestica MD02G1230000.v1.1 MD07G1082400.v1.1
prunus_persica Prupe.2G108000_v2.0.a1
pyrus_communis pycom07g06570 pycom07g06580
rosa_chinensis RchiOBHm_Chr1g0341191
rosa_laevigata RLG00000029069
rosa_multiflora Rmu_sc0004092.1_g000026 Rmu_sc0010892.1_g000010
rosa_roxburghii Rroxscaffold_4G00311790
rosa_rugosa Rorug01G0159600.1
rosa_samantha Rh1AG173900 Rh1BG141800 Rh1CG161700 Rh1DG173800
rosa_wichuraiana Rw1G014440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 354
AccBSI CCGCTC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 1018
AccII CGCG 3 cut(s) 287, 459, 999
AciI CCGC 4 cut(s) 76, 92, 235, 941
AcoI YGGCCR 2 cut(s) 147, 900
AcsI RAATTY 1 cut(s) 244
AfaI GTAC 4 cut(s) 122, 413, 587, 836
AfiI CCNNNNNNNGG 2 cut(s) 145, 950
AgsI TTSAA 7 cut(s) 66, 344, 517, 887, 935, 1090, 1128
AjnI CCWGG 1 cut(s) 270
AloI GAACNNNNNNTCC 2 cut(s) 479, 511
AluBI AGCT 8 cut(s) 170, 199, 313, 359, 375, 534, 755, 1056
AluI AGCT 8 cut(s) 170, 199, 313, 359, 375, 534, 755, 1056
Alw21I GWGCWC 1 cut(s) 536
Alw26I GTCTC 1 cut(s) 867
AlwNI CAGNNNCTG 2 cut(s) 758, 844
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 4 cut(s) 147, 575, 900, 1103
ApeKI GCWGC 4 cut(s) 45, 746, 755, 758
ApoI RAATTY 1 cut(s) 244
ArsI GACNNNNNNTTYG 4 cut(s) 95, 97, 127, 129
Asp700I GAANNNNTTC 1 cut(s) 491
AspLEI GCGC 2 cut(s) 289, 459
AspS9I GGNCC 1 cut(s) 938
AsuC2I CCSGG 3 cut(s) 453, 454, 945
AsuHPI GGTGA 1 cut(s) 805
AsuII TTCGAA 2 cut(s) 248, 548
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 1 cut(s) 938
BaeGI GKGCMC 1 cut(s) 212
BaeI ACNNNNGTAYC 2 cut(s) 395, 428
BalI TGGCCA 1 cut(s) 902
BanII GRGCYC 1 cut(s) 536
BarI GAAGNNNNNNTAC 2 cut(s) 404, 436
BbsI GAAGAC 2 cut(s) 105, 332
Bbv12I GWGCWC 1 cut(s) 536
BbvI GCAGC 4 cut(s) 57, 742, 745, 758
BccI CCATC 1 cut(s) 184
BciT130I CCWGG 1 cut(s) 272
BclI TGATCA 2 cut(s) 306, 781
BcnI CCSGG 3 cut(s) 453, 454, 945
BcoDI GTCTC 1 cut(s) 867
BfmI CTRYAG 2 cut(s) 43, 890
BisI GCNGC 4 cut(s) 46, 747, 756, 759
BlpI GCTNAGC 2 cut(s) 166, 360
BlsI GCNGC 4 cut(s) 47, 748, 757, 760
Bme1390I CCNGG 4 cut(s) 272, 453, 454, 945
Bme18I GGWCC 1 cut(s) 938
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 938
BmrFI CCNGG 4 cut(s) 272, 453, 454, 945
BmsI GCATC 1 cut(s) 445
BoxI GACNNNNGTC 1 cut(s) 800
BpiI GAAGAC 2 cut(s) 105, 332
BplI GAGNNNNNCTC 4 cut(s) 359, 391, 518, 550
BpmI CTGGAG 1 cut(s) 254
Bpu10I CCTNAGC 1 cut(s) 432
Bpu1102I GCTNAGC 2 cut(s) 166, 360
Bpu14I TTCGAA 2 cut(s) 248, 548
BpuEI CTTGAG 2 cut(s) 742, 1117
BpuMI CCSGG 3 cut(s) 453, 454, 945
BsaAI YACGTR 1 cut(s) 411
BsaJI CCNNGG 4 cut(s) 204, 452, 1106, 1118
BsaXI ACNNNNNCTCC 4 cut(s) 479, 509, 806, 836
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 950
Bse3DI GCAATG 2 cut(s) 703, 1040
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 4 cut(s) 204, 452, 1106, 1118
BseGI GGATG 3 cut(s) 5, 436, 920
BseLI CCNNNNNNNGG 2 cut(s) 145, 950
BseMI GCAATG 2 cut(s) 703, 1040
BseMII CTCAG 2 cut(s) 180, 351
BseRI GAGGAG 2 cut(s) 616, 703
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 4 cut(s) 57, 742, 745, 758
BseYI CCCAGC 1 cut(s) 415
Bsh1236I CGCG 3 cut(s) 287, 459, 999
BshFI GGCC 4 cut(s) 149, 577, 902, 1105
BsiHKAI GWGCWC 1 cut(s) 536
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 4 cut(s) 146, 453, 578, 944
BslFI GGGAC 3 cut(s) 372, 413, 417
BslI CCNNNNNNNGG 2 cut(s) 145, 950
BsmAI GTCTC 1 cut(s) 867
BsmFI GGGAC 3 cut(s) 372, 413, 417
BsmI GAATGC 1 cut(s) 1123
BsnI GGCC 4 cut(s) 149, 577, 902, 1105
BsoBI CYCGRG 1 cut(s) 452
Bsp119I TTCGAA 2 cut(s) 248, 548
Bsp1286I GDGCHC 2 cut(s) 212, 536
Bsp1407I TGTACA 2 cut(s) 120, 585
Bsp143I GATC 3 cut(s) 306, 781, 966
Bsp1720I GCTNAGC 2 cut(s) 166, 360
BspACI CCGC 4 cut(s) 76, 92, 235, 941
BspANI GGCC 4 cut(s) 149, 577, 902, 1105
BspCNI CTCAG 2 cut(s) 179, 352
BspFNI CGCG 3 cut(s) 287, 459, 999
BspMAI CTGCAG 2 cut(s) 47, 894
BspQI GCTCTTC 1 cut(s) 85
BspT104I TTCGAA 2 cut(s) 248, 548
BsrBI CCGCTC 1 cut(s) 78
BsrDI GCAATG 2 cut(s) 703, 1040
BsrGI TGTACA 2 cut(s) 120, 585
BssECI CCNNGG 4 cut(s) 204, 452, 1106, 1118
BssMI GATC 3 cut(s) 306, 781, 966
BssT1I CCWWGG 2 cut(s) 204, 1118
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 3 cut(s) 85, 950, 1106
BstAPI GCANNNNNTGC 1 cut(s) 755
BstAUI TGTACA 2 cut(s) 120, 585
BstBAI YACGTR 1 cut(s) 411
BstBI TTCGAA 2 cut(s) 248, 548
BstC8I GCNNGC 2 cut(s) 163, 894
BstDEI CTNAG 5 cut(s) 166, 360, 432, 572, 850
BstDSI CCRYGG 1 cut(s) 1106
BstF5I GGATG 3 cut(s) 5, 436, 920
BstFNI CGCG 3 cut(s) 287, 459, 999
BstHHI GCGC 2 cut(s) 289, 459
BstKTI GATC 3 cut(s) 309, 784, 969
BstMAI GTCTC 1 cut(s) 867
BstMBI GATC 3 cut(s) 306, 781, 966
BstMWI GCNNNNNNNGC 4 cut(s) 167, 752, 755, 764
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 165
BstPAI GACNNNNGTC 1 cut(s) 800
BstSCI CCNGG 4 cut(s) 270, 451, 452, 943
BstSFI CTRYAG 2 cut(s) 43, 890
BstSLI GKGCMC 1 cut(s) 212
BstUI CGCG 3 cut(s) 287, 459, 999
BstV1I GCAGC 4 cut(s) 57, 742, 745, 758
BstV2I GAAGAC 2 cut(s) 105, 332
BsuRI GGCC 4 cut(s) 149, 577, 902, 1105
BtgI CCRYGG 1 cut(s) 1106
BtsCI GGATG 3 cut(s) 5, 436, 920
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 1 cut(s) 588
Cac8I GCNNGC 2 cut(s) 163, 894
CaiI CAGNNNCTG 2 cut(s) 758, 844
CfoI GCGC 2 cut(s) 289, 459
Cfr13I GGNCC 1 cut(s) 938
Cfr9I CCCGGG 1 cut(s) 452
CseI GACGC 2 cut(s) 43, 1005
Csp6I GTAC 4 cut(s) 121, 412, 586, 835
CviAII CATG 3 cut(s) 108, 162, 1022
CviQI GTAC 4 cut(s) 121, 412, 586, 835
DdeI CTNAG 5 cut(s) 166, 360, 432, 572, 850
DpnI GATC 3 cut(s) 308, 783, 968
DpnII GATC 3 cut(s) 306, 781, 966
EaeI YGGCCR 2 cut(s) 147, 900
Eam1104I CTCTTC 3 cut(s) 85, 950, 1106
EarI CTCTTC 3 cut(s) 85, 950, 1106
EciI GGCGGA 1 cut(s) 250
Ecl136II GAGCTC 1 cut(s) 534
Eco130I CCWWGG 2 cut(s) 204, 1118
Eco24I GRGCYC 1 cut(s) 536
Eco47I GGWCC 1 cut(s) 938
Eco53kI GAGCTC 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 534
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 204, 1118
EcoT38I GRGCYC 1 cut(s) 536
ErhI CCWWGG 2 cut(s) 204, 1118
FaeI CATG 3 cut(s) 111, 165, 1025
FalI AAGNNNNNCTT 8 cut(s) 188, 220, 324, 356, 454, 486, 704, 736
FaqI GGGAC 3 cut(s) 372, 413, 417
FatI CATG 3 cut(s) 107, 161, 1021
FauI CCCGC 2 cut(s) 83, 99
FauNDI CATATG 1 cut(s) 863
FbaI TGATCA 2 cut(s) 306, 781
FblI GTMKAC 1 cut(s) 1018
Fnu4HI GCNGC 4 cut(s) 46, 747, 756, 759
FokI GGATG 2 cut(s) 423, 927
FriOI GRGCYC 1 cut(s) 536
Fsp4HI GCNGC 4 cut(s) 46, 747, 756, 759
GlaI GCGC 2 cut(s) 288, 458
GluI GCNGC 4 cut(s) 46, 747, 756, 759
GsaI CCCAGC 1 cut(s) 419
GsuI CTGGAG 1 cut(s) 254
HaeIII GGCC 4 cut(s) 149, 577, 902, 1105
HapII CCGG 4 cut(s) 146, 453, 578, 944
HgaI GACGC 2 cut(s) 43, 1005
HhaI GCGC 2 cut(s) 289, 459
Hin1II CATG 3 cut(s) 111, 165, 1025
Hin6I GCGC 2 cut(s) 287, 457
HinP1I GCGC 2 cut(s) 287, 457
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 4 cut(s) 405, 701, 1025, 1114
HpaII CCGG 4 cut(s) 146, 453, 578, 944
HphI GGTGA 1 cut(s) 805
Hpy166II GTNNAC 7 cut(s) 99, 123, 186, 277, 588, 920, 1019
Hpy188I TCNGA 2 cut(s) 10, 221
Hpy188III TCNNGA 2 cut(s) 344, 983
Hpy8I GTNNAC 7 cut(s) 99, 123, 186, 277, 588, 920, 1019
HpyAV CCTTC 5 cut(s) 72, 430, 511, 670, 929
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 2 cut(s) 291, 410
HpyCH4V TGCA 6 cut(s) 31, 45, 161, 540, 867, 892
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 752, 755, 764
HpyF3I CTNAG 5 cut(s) 166, 360, 432, 572, 850
HpySE526I ACGT 2 cut(s) 291, 410
Hsp92II CATG 3 cut(s) 111, 165, 1025
HspAI GCGC 2 cut(s) 287, 457
Ksp22I TGATCA 2 cut(s) 306, 781
Kzo9I GATC 3 cut(s) 306, 781, 966
LguI GCTCTTC 1 cut(s) 85
LmnI GCTCC 3 cut(s) 175, 531, 743
Lsp1109I GCAGC 4 cut(s) 57, 742, 745, 758
LweI GCATC 1 cut(s) 445
MaeII ACGT 2 cut(s) 291, 410
MaeIII GTNAC 2 cut(s) 640, 793
MalI GATC 3 cut(s) 308, 783, 968
MbiI CCGCTC 1 cut(s) 78
MboI GATC 3 cut(s) 306, 781, 966
MboII GAAGA 7 cut(s) 72, 105, 332, 866, 967, 1056, 1123
MhlI GDGCHC 2 cut(s) 212, 536
MlsI TGGCCA 1 cut(s) 902
MluCI AATT 4 cut(s) 244, 618, 786, 882
MluNI TGGCCA 1 cut(s) 902
MlyI GAGTC 2 cut(s) 399, 1123
MmeI TCCRAC 1 cut(s) 33
Mox20I TGGCCA 1 cut(s) 902
MroXI GAANNNNTTC 1 cut(s) 491
MscI TGGCCA 1 cut(s) 902
MseI TTAA 3 cut(s) 621, 789, 1077
Msp20I TGGCCA 1 cut(s) 902
MspA1I CMGCKG 1 cut(s) 755
MspI CCGG 4 cut(s) 146, 453, 578, 944
MspR9I CCNGG 4 cut(s) 272, 453, 454, 945
Mva1269I GAATGC 1 cut(s) 1123
MvaI CCWGG 1 cut(s) 272
MvnI CGCG 3 cut(s) 287, 459, 999
MwoI GCNNNNNNNGC 4 cut(s) 167, 752, 755, 764
NciI CCSGG 3 cut(s) 453, 454, 945
NdeI CATATG 1 cut(s) 863
NdeII GATC 3 cut(s) 306, 781, 966
NlaIII CATG 3 cut(s) 111, 165, 1025
NmeAIII GCCGAG 1 cut(s) 798
NmuCI GTSAC 1 cut(s) 793
NspI RCATGY 1 cut(s) 165
NspV TTCGAA 2 cut(s) 248, 548
PaeI GCATGC 1 cut(s) 165
PciSI GCTCTTC 1 cut(s) 85
PcsI WCGNNNNNNNCGW 1 cut(s) 770
PctI GAATGC 1 cut(s) 1123
PdmI GAANNNNTTC 1 cut(s) 491
PfeI GAWTC 2 cut(s) 701, 1025
PflFI GACNNNGTC 1 cut(s) 109
PkrI GCNGC 4 cut(s) 47, 748, 757, 760
PleI GAGTC 2 cut(s) 399, 1122
PpsI GAGTC 2 cut(s) 399, 1122
Ppu21I YACGTR 1 cut(s) 411
PshAI GACNNNNGTC 1 cut(s) 800
PsiI TTATAA 1 cut(s) 354
Psp124BI GAGCTC 1 cut(s) 536
Psp6I CCWGG 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 415
PspGI CCWGG 1 cut(s) 270
PspPI GGNCC 1 cut(s) 938
PstI CTGCAG 2 cut(s) 47, 894
PstNI CAGNNNCTG 2 cut(s) 758, 844
PsyI GACNNNGTC 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 4 cut(s) 122, 413, 587, 836
RsaNI GTAC 4 cut(s) 121, 412, 586, 835
SacI GAGCTC 1 cut(s) 536
SapI GCTCTTC 1 cut(s) 85
SaqAI TTAA 3 cut(s) 621, 789, 1077
SatI GCNGC 4 cut(s) 46, 747, 756, 759
Sau3AI GATC 3 cut(s) 306, 781, 966
Sau96I GGNCC 1 cut(s) 938
SchI GAGTC 2 cut(s) 399, 1123
ScrFI CCNGG 4 cut(s) 272, 453, 454, 945
SduI GDGCHC 2 cut(s) 212, 536
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 2 cut(s) 43, 890
SfuI TTCGAA 2 cut(s) 248, 548
SinI GGWCC 1 cut(s) 938
SmaI CCCGGG 1 cut(s) 454
SmlI CTYRAG 2 cut(s) 721, 1132
SmoI CTYRAG 2 cut(s) 721, 1132
SphI GCATGC 1 cut(s) 165
Sse9I AATT 4 cut(s) 244, 618, 786, 882
SsiI CCGC 4 cut(s) 76, 92, 235, 941
SstI GAGCTC 1 cut(s) 536
StyD4I CCNGG 4 cut(s) 270, 451, 452, 943
StyI CCWWGG 2 cut(s) 204, 1118
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 2 cut(s) 294, 413
TaqI TCGA 5 cut(s) 179, 248, 548, 635, 982
TasI AATT 4 cut(s) 244, 618, 786, 882
TatI WGTACW 2 cut(s) 120, 585
TfiI GAWTC 2 cut(s) 701, 1025
Tru1I TTAA 3 cut(s) 621, 789, 1077
Tru9I TTAA 3 cut(s) 621, 789, 1077
TscAI CASTG 1 cut(s) 595
TseFI GTSAC 1 cut(s) 793
TseI GCWGC 4 cut(s) 45, 746, 755, 758
Tsp45I GTSAC 1 cut(s) 793
TspDTI ATGAA 4 cut(s) 38, 714, 850, 916
TspGWI ACGGA 1 cut(s) 1123
TspMI CCCGGG 1 cut(s) 452
TspRI CASTG 1 cut(s) 595
Tth111I GACNNNGTC 1 cut(s) 109
VpaK11BI GGWCC 1 cut(s) 938
XapI RAATTY 1 cut(s) 244
XceI RCATGY 1 cut(s) 165
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XmaI CCCGGG 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 1018
XmnI GAANNNNTTC 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.