Rmu_sc0004092.1_g000026

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004092.1
OG0014794
Physical Location & Seq
Reverse (-)
98476 .. 102500
4025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004092.1_g000026.1.cds

Sequence Viewer

Length: 1203 bp
ctggctgccgagcttgagaccaaatatagtccatactacattcgaggactcacatatccggcagctcttgtacctcctagtcctggaccaggcgaaagcgaaccatcttctggtgctacttggacctctatctcaactttcttgtttaaaatgagggagtggagctcttgccgtgtcagctctaagaaaggtcagctttacagcaagaagcttaaggctcaagcaacctacactgcagtgaaatctaggcgtcctcaaccaataacacctaatgaactgtcagttaattcagaaacatcgaatatggtggtaaccgaagacatcatcagcactgttgaaaacaaagagaagccattgagggagagagtacttgaagcagtagcaacagctgctacaccaacaaataccgaaagggagtacatatgggcagacaagtaccagccccagtgcttagaagatttcatatgtaatagagacaaagcaactcagctacaggttttggccagaggtggagggtgtggccatttcatattcgaaggacctccaggcgtggggaagagaactatgatctgggctatgctgagagaggcttttggacgtgacactatacatggtgaagtggtaggtagtattaatgtacaagtgaagcaatcccctcaacatgtagaagtaaatctctctgaactgaaaggttatgagaagcatgtgattgttgaactcatgaaggaaacacaatacaacatacctaacaaatcagctttgccgtgcagccttgacaactgccgagacaagctatccacagatgccctactttatattaagtggctattagaaaggtataaagggtgtaataaggtcttcttctgctgttctgatgtggcaaagcttcaggccatcaagacaatttgtactgtcgttgagcttttgccgccttcaaagaatgagattgttgaagttttaaaatttatagccaaacaagaaggcatagatttgccacatcaactggctgaaaggtttgctgaaaattccaaaaacagtctccgtctggcaattcgttcattcgaggccacttggaaaaagggttcttatccctttaaagaagatcaagtaattttgacaggatgggaggatgaaattgcagatattggaaagaatatgatcgaagagcaaagcccaaaacagtatgttcatctgaaattttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
No domains found.

Protein Analysis

400

Amino Acids

45.11

Weight (kDa)

8.22

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014794)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08091
malus_domestica MD02G1230000.v1.1 MD07G1082400.v1.1
prunus_persica Prupe.2G108000_v2.0.a1
pyrus_communis pycom07g06570 pycom07g06580
rosa_chinensis RchiOBHm_Chr1g0341191
rosa_laevigata RLG00000029069
rosa_multiflora Rmu_sc0004092.1_g000026 Rmu_sc0010892.1_g000010
rosa_roxburghii Rroxscaffold_4G00311790
rosa_rugosa Rorug01G0159600.1
rosa_samantha Rh1AG173900 Rh1BG141800 Rh1CG161700 Rh1DG173800
rosa_wichuraiana Rw1G014440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 110
AciI CCGC 1 cut(s) 929
AcoI YGGCCR 2 cut(s) 501, 520
AcsI RAATTY 3 cut(s) 962, 1024, 1196
AcuI CTGAAG 1 cut(s) 872
AcyI GRCGYC 1 cut(s) 250
AfaI GTAC 6 cut(s) 72, 369, 419, 437, 639, 910
AfiI CCNNNNNNNGG 4 cut(s) 83, 89, 110, 551
AflII CTTAAG 1 cut(s) 212
AflIII ACRYGT 1 cut(s) 661
AgsI TTSAA 5 cut(s) 338, 374, 716, 936, 953
AjiI CACGTC 1 cut(s) 599
AjnI CCWGG 3 cut(s) 82, 88, 544
AleI CACNNNNGTG 1 cut(s) 236
AloI GAACNNNNNNTCC 2 cut(s) 1066, 1098
Alw21I GWGCWC 1 cut(s) 167
Alw26I GTCTC 4 cut(s) 11, 468, 780, 1043
AoxI GGCC 4 cut(s) 501, 520, 891, 1065
ApeKI GCWGC 4 cut(s) 5, 62, 389, 768
ApoI RAATTY 3 cut(s) 962, 1024, 1196
AseI ATTAAT 1 cut(s) 633
AspS9I GGNCC 3 cut(s) 86, 123, 539
AsuHPI GGTGA 1 cut(s) 626
AsuII TTCGAA 1 cut(s) 534
AvaII GGWCC 3 cut(s) 86, 123, 539
BalI TGGCCA 2 cut(s) 503, 522
BanII GRGCYC 1 cut(s) 167
BbsI GAAGAC 2 cut(s) 324, 850
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 3 cut(s) 74, 376, 780
BccI CCATC 3 cut(s) 112, 902, 1116
BceAI ACGGC 2 cut(s) 156, 748
BciT130I CCWGG 3 cut(s) 84, 90, 546
BcoDI GTCTC 4 cut(s) 11, 468, 780, 1043
BfaI CTAG 2 cut(s) 78, 246
BfmI CTRYAG 2 cut(s) 234, 491
BfrI CTTAAG 1 cut(s) 212
BisI GCNGC 5 cut(s) 6, 63, 390, 769, 929
BlsI GCNGC 5 cut(s) 7, 64, 391, 770, 930
BmcAI AGTACT 1 cut(s) 369
Bme1390I CCNGG 3 cut(s) 84, 90, 546
Bme18I GGWCC 3 cut(s) 86, 123, 539
BmgBI CACGTC 1 cut(s) 599
BmgT120I GGNCC 3 cut(s) 86, 123, 539
BmrFI CCNGG 3 cut(s) 84, 90, 546
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 1 cut(s) 793
BmuI ACTGGG 1 cut(s) 439
BpiI GAAGAC 2 cut(s) 324, 850
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpmI CTGGAG 1 cut(s) 528
Bpu14I TTCGAA 1 cut(s) 534
BpuEI CTTGAG 2 cut(s) 35, 204
BsaHI GRCGYC 1 cut(s) 250
BsaI GGTCTC 1 cut(s) 11
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 89, 110, 551
Bse1I ACTGG 2 cut(s) 445, 1008
BseBI CCWGG 3 cut(s) 84, 90, 546
BseGI GGATG 2 cut(s) 1127, 1135
BseLI CCNNNNNNNGG 4 cut(s) 83, 89, 110, 551
BseMII CTCAG 2 cut(s) 500, 572
BseNI ACTGG 2 cut(s) 445, 1008
BseXI GCAGC 3 cut(s) 74, 376, 780
BsgI GTGCAG 1 cut(s) 787
BshFI GGCC 4 cut(s) 503, 522, 893, 1067
BsiHKAI GWGCWC 1 cut(s) 167
BsiSI CCGG 1 cut(s) 59
BslI CCNNNNNNNGG 4 cut(s) 83, 89, 110, 551
BsmAI GTCTC 4 cut(s) 11, 468, 780, 1043
BsnI GGCC 4 cut(s) 503, 522, 893, 1067
Bso31I GGTCTC 1 cut(s) 11
Bsp119I TTCGAA 1 cut(s) 534
Bsp1286I GDGCHC 1 cut(s) 167
Bsp1407I TGTACA 1 cut(s) 637
Bsp143I GATC 3 cut(s) 567, 1102, 1158
BspACI CCGC 1 cut(s) 929
BspANI GGCC 4 cut(s) 503, 522, 893, 1067
BspCNI CTCAG 2 cut(s) 499, 573
BspHI TCATGA 1 cut(s) 720
BspMAI CTGCAG 1 cut(s) 238
BspQI GCTCTTC 1 cut(s) 1158
BspT104I TTCGAA 1 cut(s) 534
BspTI CTTAAG 1 cut(s) 212
BspTNI GGTCTC 1 cut(s) 11
BsrGI TGTACA 1 cut(s) 637
BsrI ACTGG 2 cut(s) 445, 1008
BssMI GATC 3 cut(s) 567, 1102, 1158
BssNI GRCGYC 1 cut(s) 250
Bst2UI CCWGG 3 cut(s) 84, 90, 546
Bst4CI ACNGT 5 cut(s) 279, 334, 913, 1037, 1182
Bst6I CTCTTC 2 cut(s) 551, 1158
BstACI GRCGYC 1 cut(s) 250
BstAFI CTTAAG 1 cut(s) 212
BstAPI GCANNNNNTGC 1 cut(s) 389
BstAUI TGTACA 1 cut(s) 637
BstBI TTCGAA 1 cut(s) 534
BstDEI CTNAG 4 cut(s) 183, 451, 486, 581
BstEII GGTNACC 1 cut(s) 310
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 1127, 1135
BstKTI GATC 3 cut(s) 570, 1105, 1161
BstMAI GTCTC 4 cut(s) 11, 468, 780, 1043
BstMBI GATC 3 cut(s) 567, 1102, 1158
BstMWI GCNNNNNNNGC 3 cut(s) 177, 389, 928
BstNI CCWGG 3 cut(s) 84, 90, 546
BstNSI RCATGY 2 cut(s) 665, 707
BstPI GGTNACC 1 cut(s) 310
BstSCI CCNGG 3 cut(s) 82, 88, 544
BstSFI CTRYAG 2 cut(s) 234, 491
BstV1I GCAGC 3 cut(s) 74, 376, 780
BstV2I GAAGAC 2 cut(s) 324, 850
BsuRI GGCC 4 cut(s) 503, 522, 893, 1067
BtrI CACGTC 1 cut(s) 599
BtsCI GGATG 2 cut(s) 1127, 1135
BtsI GCAGTG 2 cut(s) 231, 243
BtsIMutI CAGTG 4 cut(s) 231, 243, 330, 452
CciI TCATGA 1 cut(s) 720
Cfr13I GGNCC 3 cut(s) 86, 123, 539
CseI GACGC 1 cut(s) 239
Csp6I GTAC 6 cut(s) 71, 368, 418, 436, 638, 909
CviAII CATG 4 cut(s) 611, 662, 704, 721
CviQI GTAC 6 cut(s) 71, 368, 418, 436, 638, 909
DdeI CTNAG 4 cut(s) 183, 451, 486, 581
DpnI GATC 3 cut(s) 569, 1104, 1160
DpnII GATC 3 cut(s) 567, 1102, 1158
DraI TTTAAA 3 cut(s) 148, 960, 1096
EaeI YGGCCR 2 cut(s) 501, 520
Eam1104I CTCTTC 2 cut(s) 551, 1158
EarI CTCTTC 2 cut(s) 551, 1158
Ecl136II GAGCTC 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 167
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 3 cut(s) 86, 123, 539
Eco53kI GAGCTC 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 872
Eco91I GGTNACC 1 cut(s) 310
EcoICRI GAGCTC 1 cut(s) 165
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 539
EcoO65I GGTNACC 1 cut(s) 310
EcoRII CCWGG 3 cut(s) 82, 88, 544
EcoT38I GRGCYC 1 cut(s) 167
FaeI CATG 4 cut(s) 614, 665, 707, 724
FalI AAGNNNNNCTT 4 cut(s) 180, 212, 845, 877
FatI CATG 4 cut(s) 610, 661, 703, 720
FauNDI CATATG 2 cut(s) 422, 464
Fnu4HI GCNGC 5 cut(s) 6, 63, 390, 769, 929
FokI GGATG 2 cut(s) 1134, 1142
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 5 cut(s) 6, 63, 390, 769, 929
FspBI CTAG 2 cut(s) 78, 246
GluI GCNGC 5 cut(s) 6, 63, 390, 769, 929
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 4 cut(s) 503, 522, 893, 1067
HapII CCGG 1 cut(s) 59
HgaI GACGC 1 cut(s) 239
Hin1I GRCGYC 1 cut(s) 250
Hin1II CATG 4 cut(s) 614, 665, 707, 724
HindIII AAGCTT 2 cut(s) 209, 884
HinfI GANTC 1 cut(s) 48
HpaII CCGG 1 cut(s) 59
HphI GGTGA 1 cut(s) 626
Hpy188I TCNGA 4 cut(s) 292, 682, 874, 1194
Hpy188III TCNNGA 2 cut(s) 721, 898
HpyAV CCTTC 4 cut(s) 530, 718, 942, 974
HpyCH4III ACNGT 5 cut(s) 279, 334, 913, 1037, 1182
HpyCH4IV ACGT 1 cut(s) 598
HpyCH4V TGCA 3 cut(s) 236, 768, 1139
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 389, 928
HpyF3I CTNAG 4 cut(s) 183, 451, 486, 581
HpySE526I ACGT 1 cut(s) 598
Hsp92I GRCGYC 1 cut(s) 250
Hsp92II CATG 4 cut(s) 614, 665, 707, 724
Kzo9I GATC 3 cut(s) 567, 1102, 1158
LguI GCTCTTC 1 cut(s) 1158
LmnI GCTCC 1 cut(s) 162
Lsp1109I GCAGC 3 cut(s) 74, 376, 780
LweI GCATC 1 cut(s) 793
MaeI CTAG 2 cut(s) 78, 246
MaeII ACGT 1 cut(s) 598
MaeIII GTNAC 2 cut(s) 310, 599
MalI GATC 3 cut(s) 569, 1104, 1160
MboI GATC 3 cut(s) 567, 1102, 1158
MboII GAAGA 8 cut(s) 99, 329, 467, 568, 850, 853, 1112, 1175
MhlI GDGCHC 1 cut(s) 167
MlsI TGGCCA 2 cut(s) 503, 522
MluCI AATT 8 cut(s) 286, 903, 962, 1024, 1050, 1110, 1134, 1196
MluNI TGGCCA 2 cut(s) 503, 522
MlyI GAGTC 1 cut(s) 42
Mox20I TGGCCA 2 cut(s) 503, 522
MscI TGGCCA 2 cut(s) 503, 522
MseI TTAA 7 cut(s) 147, 213, 285, 633, 819, 959, 1095
MslI CAYNNNNRTG 1 cut(s) 236
Msp20I TGGCCA 2 cut(s) 503, 522
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 212
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 3 cut(s) 84, 90, 546
MvaI CCWGG 3 cut(s) 84, 90, 546
MwoI GCNNNNNNNGC 3 cut(s) 177, 389, 928
NdeI CATATG 2 cut(s) 422, 464
NdeII GATC 3 cut(s) 567, 1102, 1158
NlaIII CATG 4 cut(s) 614, 665, 707, 724
NmeAIII GCCGAG 2 cut(s) 34, 809
NmuCI GTSAC 1 cut(s) 599
NspI RCATGY 2 cut(s) 665, 707
NspV TTCGAA 1 cut(s) 534
OliI CACNNNNGTG 1 cut(s) 236
PagI TCATGA 1 cut(s) 720
PciI ACATGT 1 cut(s) 661
PciSI GCTCTTC 1 cut(s) 1158
PflMI CCANNNNNTGG 1 cut(s) 110
PfoI TCCNGGA 1 cut(s) 82
PkrI GCNGC 5 cut(s) 7, 64, 391, 770, 930
PleI GAGTC 1 cut(s) 42
PpsI GAGTC 1 cut(s) 42
PpuMI RGGWCCY 1 cut(s) 539
PscI ACATGT 1 cut(s) 661
PshBI ATTAAT 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 539
Psp6I CCWGG 3 cut(s) 82, 88, 544
PspEI GGTNACC 1 cut(s) 310
PspGI CCWGG 3 cut(s) 82, 88, 544
PspPI GGNCC 3 cut(s) 86, 123, 539
PspPPI RGGWCCY 1 cut(s) 539
PstI CTGCAG 1 cut(s) 238
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 6 cut(s) 72, 369, 419, 437, 639, 910
RsaNI GTAC 6 cut(s) 71, 368, 418, 436, 638, 909
RseI CAYNNNNRTG 1 cut(s) 236
SacI GAGCTC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 1158
SaqAI TTAA 7 cut(s) 147, 213, 285, 633, 819, 959, 1095
SatI GCNGC 5 cut(s) 6, 63, 390, 769, 929
Sau3AI GATC 3 cut(s) 567, 1102, 1158
Sau96I GGNCC 3 cut(s) 86, 123, 539
ScaI AGTACT 1 cut(s) 369
SchI GAGTC 1 cut(s) 42
ScrFI CCNGG 3 cut(s) 84, 90, 546
SduI GDGCHC 1 cut(s) 167
SfaNI GCATC 1 cut(s) 793
SfcI CTRYAG 2 cut(s) 234, 491
SfuI TTCGAA 1 cut(s) 534
SinI GGWCC 3 cut(s) 86, 123, 539
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 3 cut(s) 14, 212, 219
SmoI CTYRAG 3 cut(s) 14, 212, 219
Sse9I AATT 8 cut(s) 286, 903, 962, 1024, 1050, 1110, 1134, 1196
SsiI CCGC 1 cut(s) 929
SspMI CTAG 2 cut(s) 78, 246
SstI GAGCTC 1 cut(s) 167
StyD4I CCNGG 3 cut(s) 82, 88, 544
TaaI ACNGT 5 cut(s) 279, 334, 913, 1037, 1182
TaiI ACGT 1 cut(s) 601
TaqI TCGA 5 cut(s) 43, 299, 534, 1062, 1161
TasI AATT 8 cut(s) 286, 903, 962, 1024, 1050, 1110, 1134, 1196
TatI WGTACW 4 cut(s) 367, 417, 637, 908
TauI GCSGC 1 cut(s) 931
Tru1I TTAA 7 cut(s) 147, 213, 285, 633, 819, 959, 1095
Tru9I TTAA 7 cut(s) 147, 213, 285, 633, 819, 959, 1095
TscAI CASTG 4 cut(s) 238, 243, 337, 452
TseFI GTSAC 1 cut(s) 599
TseI GCWGC 4 cut(s) 5, 62, 389, 768
Tsp45I GTSAC 1 cut(s) 599
TspDTI ATGAA 7 cut(s) 288, 451, 517, 737, 1047, 1146, 1178
TspGWI ACGGA 1 cut(s) 1031
TspRI CASTG 4 cut(s) 238, 243, 337, 452
Van91I CCANNNNNTGG 1 cut(s) 110
Vha464I CTTAAG 1 cut(s) 212
VpaK11BI GGWCC 3 cut(s) 86, 123, 539
VspI ATTAAT 1 cut(s) 633
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 3 cut(s) 962, 1024, 1196
XceI RCATGY 2 cut(s) 665, 707
XspI CTAG 2 cut(s) 78, 246
ZrmI AGTACT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.