pycom07g14210

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009202
Physical Location & Seq
Reverse (-)
16163438 .. 16166071
2634 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
ATGAGCCTGTGTGTACCAAAAATTTGTGTCCTCCACAAGATTTTAATCGTATGGATGACAGTGGTGGCAACTTTGAGTTGTTTAGGTCACTCTAAATGTGAATTCGAGGGAATTTTCAATTTTGGAGATTCGAACTCAGATACAGGAGGATTTTGGGCAGCTTTTCCGGCGCAATCTGGACCCAATGGCATGACATACTTCAAGAGACCTGTTGGTCGGGCTACAGATGGGAGGCTCATTGTTGATTTTCTAGCTCAAGCTCTAGGGTTACCATTTATAAGTCCATACCTGAAATCAATTGGATCTGATTATAAACATGGGGCAAACTATGCAACACTAGCATCCACTGTGCTTTTGCCAAACACTTCCTTGTTTGTCACTGGAATCAGCCCATTCTCTCTGGCCATCCAGCTCAACCAAATGAAGGCATTCAAGACCCAAGTTGAAGAGTTTCACCACTCCTCGAATCAACAAGAACTGAAGAGACTTCCCTCCCTGGATATTTTTGGGAAATCACTCTACACATTCTACATTGGCCAAAACGATTTCACCTCCAATTTGGCAGCCATTGGTGTAGGTGGAGTGAAGCAATATCTTCCTCAAGTGGTCTCTCAAATTGCTGGTACCATCAAGGAGCTATATGCGTTAGGAGGGCGTGCATTTCTGGTACTTAATCTGGCACCAGTGGGTTGTTATCCATCATTTTTGGCGGGGCTTCCTCCTTCTGAACTCGACGCATTTGGATGCTCAATTGCTTACAACAACGCAGTCGTGGACTACAACAAGTTGTTGAAGGAGGCACTTAGCCAAACTAGAGGCTCCCTCCCAAATGGTTCCCTCATATATGTGGATACCTCCTCTGTTTTGCTTGAGCTCTTTAGGCATCCCACATCTCATGGCTTGAAGTATGGTACAAAATCATGTTGTGGGCATGGTGGTGGTGCCCATAATTTTGACCCTCAAGTTTTTTGTGGAAATACAAAGGTGATCAATGGGAGACCTGTGACTGCAACTGCTTGTAGTGACCCTTACAACTATGTGAGCTGGGATGGAGTCCATGCCACAGAAGCTGCAAACAAGCTAACAACATTGGCTATCCTCAACGGCACTTTCTCAGACCCTCCCCTTCCCTTTGAGCAGTTCTGTGACCTCCACAGTATCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

389

Amino Acids

41.8

Weight (kDa)

7.13

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 362 3.4e-41 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 278, 312
AasI GACNNNNNNGTC 1 cut(s) 213
Acc65I GGTACC 1 cut(s) 623
AccB1I GGYRCC 3 cut(s) 623, 679, 941
AciI CCGC 1 cut(s) 710
AclWI GGATC 1 cut(s) 310
AcoI YGGCCR 2 cut(s) 402, 535
AcsI RAATTY 3 cut(s) 21, 101, 111
AcuI CTGAAG 1 cut(s) 500
AfaI GTAC 4 cut(s) 15, 625, 669, 913
AfiI CCNNNNNNNGG 1 cut(s) 424
AgsI TTSAA 6 cut(s) 118, 202, 433, 446, 793, 904
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 9 cut(s) 161, 254, 260, 412, 637, 874, 1044, 1070, 1081
AluI AGCT 9 cut(s) 161, 254, 260, 412, 637, 874, 1044, 1070, 1081
Alw21I GWGCWC 1 cut(s) 876
Alw26I GTCTC 4 cut(s) 199, 478, 613, 991
AlwI GGATC 1 cut(s) 310
AlwNI CAGNNNCTG 1 cut(s) 1070
AoxI GGCC 2 cut(s) 402, 535
ApeKI GCWGC 3 cut(s) 158, 563, 1070
ApoI RAATTY 3 cut(s) 21, 101, 111
Asp700I GAANNNNTTC 2 cut(s) 428, 450
Asp718I GGTACC 1 cut(s) 623
AspLEI GCGC 1 cut(s) 172
AspS9I GGNCC 1 cut(s) 179
AsuHPI GGTGA 3 cut(s) 446, 541, 997
AsuII TTCGAA 1 cut(s) 131
AvaII GGWCC 1 cut(s) 179
BaeGI GKGCMC 1 cut(s) 946
BalI TGGCCA 2 cut(s) 404, 537
BanI GGYRCC 3 cut(s) 623, 679, 941
BanII GRGCYC 1 cut(s) 876
Bbv12I GWGCWC 1 cut(s) 876
BbvI GCAGC 3 cut(s) 170, 575, 1057
BccI CCATC 5 cut(s) 221, 413, 635, 706, 1043
BceAI ACGGC 1 cut(s) 1120
BciT130I CCWGG 1 cut(s) 497
BciVI GTATCC 1 cut(s) 844
BclI TGATCA 1 cut(s) 987
BcoDI GTCTC 4 cut(s) 199, 478, 613, 991
BfaI CTAG 4 cut(s) 251, 263, 338, 813
BfmI CTRYAG 1 cut(s) 222
BfuI GTATCC 1 cut(s) 844
BisI GCNGC 3 cut(s) 159, 564, 1071
BlsI GCNGC 3 cut(s) 160, 565, 1072
Bme1390I CCNGG 1 cut(s) 497
Bme18I GGWCC 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 179
BmiI GGNNCC 6 cut(s) 181, 625, 681, 820, 835, 943
BmrFI CCNGG 1 cut(s) 497
BmsI GCATC 3 cut(s) 350, 734, 892
BplI GAGNNNNNCTC 2 cut(s) 807, 839
Bpu14I TTCGAA 1 cut(s) 131
BpuEI CTTGAG 4 cut(s) 240, 585, 890, 945
BsaBI GATNNNNATC 1 cut(s) 44
BsaI GGTCTC 3 cut(s) 199, 613, 991
BsaJI CCNNGG 1 cut(s) 495
Bsc4I CCNNNNNNNGG 1 cut(s) 424
Bse1I ACTGG 2 cut(s) 385, 683
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 6 cut(s) 60, 341, 405, 749, 883, 1054
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 1 cut(s) 424
BseMII CTCAG 2 cut(s) 150, 1128
BseNI ACTGG 2 cut(s) 385, 683
BseRI GAGGAG 2 cut(s) 451, 847
BseSI GKGCMC 1 cut(s) 946
BseXI GCAGC 3 cut(s) 170, 575, 1057
BseYI CCCAGC 1 cut(s) 1044
BshFI GGCC 2 cut(s) 404, 537
BshNI GGYRCC 3 cut(s) 623, 679, 941
BsiHKAI GWGCWC 1 cut(s) 876
BsiSI CCGG 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 424
BsmAI GTCTC 4 cut(s) 199, 478, 613, 991
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 2 cut(s) 404, 537
Bso31I GGTCTC 3 cut(s) 199, 613, 991
Bsp119I TTCGAA 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 876, 946
Bsp143I GATC 2 cut(s) 302, 987
BspACI CCGC 1 cut(s) 710
BspANI GGCC 2 cut(s) 404, 537
BspCNI CTCAG 2 cut(s) 149, 1127
BspLI GGNNCC 6 cut(s) 181, 625, 681, 820, 835, 943
BspPI GGATC 1 cut(s) 310
BspT104I TTCGAA 1 cut(s) 131
BspT107I GGYRCC 3 cut(s) 623, 679, 941
BspTNI GGTCTC 3 cut(s) 199, 613, 991
BsrI ACTGG 2 cut(s) 385, 683
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 2 cut(s) 302, 987
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 3 cut(s) 61, 349, 1157
Bst6I CTCTTC 2 cut(s) 441, 476
BstAPI GCANNNNNTGC 1 cut(s) 329
BstBI TTCGAA 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 657
BstDEI CTNAG 3 cut(s) 136, 803, 1114
BstEII GGTNACC 1 cut(s) 267
BstF5I GGATG 6 cut(s) 60, 341, 405, 749, 883, 1054
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 2 cut(s) 305, 990
BstMAI GTCTC 4 cut(s) 199, 478, 613, 991
BstMBI GATC 2 cut(s) 302, 987
BstMWI GCNNNNNNNGC 5 cut(s) 167, 329, 338, 880, 1067
BstNI CCWGG 1 cut(s) 497
BstPI GGTNACC 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 495
BstSFI CTRYAG 1 cut(s) 222
BstSLI GKGCMC 1 cut(s) 946
BstV1I GCAGC 3 cut(s) 170, 575, 1057
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BsuI GTATCC 1 cut(s) 844
BsuRI GGCC 2 cut(s) 404, 537
BtsCI GGATG 6 cut(s) 60, 341, 405, 749, 883, 1054
BtsIMutI CAGTG 4 cut(s) 66, 345, 378, 690
Cac8I GCNNGC 1 cut(s) 657
CaiI CAGNNNCTG 1 cut(s) 1070
CfoI GCGC 1 cut(s) 172
Cfr13I GGNCC 1 cut(s) 179
CseI GACGC 1 cut(s) 743
Csp6I GTAC 4 cut(s) 14, 624, 668, 912
CviAII CATG 6 cut(s) 190, 317, 896, 921, 932, 1058
CviQI GTAC 4 cut(s) 14, 624, 668, 912
DdeI CTNAG 3 cut(s) 136, 803, 1114
DpnI GATC 2 cut(s) 304, 989
DpnII GATC 2 cut(s) 302, 987
DrdI GACNNNNNNGTC 1 cut(s) 213
DseDI GACNNNNNNGTC 1 cut(s) 213
EaeI YGGCCR 2 cut(s) 402, 535
Eam1104I CTCTTC 2 cut(s) 441, 476
EarI CTCTTC 2 cut(s) 441, 476
Ecl136II GAGCTC 1 cut(s) 874
Eco24I GRGCYC 1 cut(s) 876
Eco31I GGTCTC 3 cut(s) 199, 613, 991
Eco47I GGWCC 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 874
Eco57I CTGAAG 1 cut(s) 500
Eco91I GGTNACC 1 cut(s) 267
EcoICRI GAGCTC 1 cut(s) 874
EcoO65I GGTNACC 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 495
EcoT38I GRGCYC 1 cut(s) 876
FaeI CATG 6 cut(s) 193, 320, 899, 924, 935, 1061
FatI CATG 6 cut(s) 189, 316, 895, 920, 931, 1057
FauI CCCGC 1 cut(s) 703
FbaI TGATCA 1 cut(s) 987
Fnu4HI GCNGC 3 cut(s) 159, 564, 1071
FokI GGATG 6 cut(s) 67, 328, 392, 756, 870, 1061
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 3 cut(s) 159, 564, 1071
FspBI CTAG 4 cut(s) 251, 263, 338, 813
GlaI GCGC 1 cut(s) 171
GluI GCNGC 3 cut(s) 159, 564, 1071
GsaI CCCAGC 1 cut(s) 1048
HaeIII GGCC 2 cut(s) 404, 537
HapII CCGG 1 cut(s) 167
HgaI GACGC 1 cut(s) 743
HhaI GCGC 1 cut(s) 172
Hin1II CATG 6 cut(s) 193, 320, 899, 924, 935, 1061
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HinfI GANTC 4 cut(s) 128, 384, 466, 1053
HpaII CCGG 1 cut(s) 167
HphI GGTGA 3 cut(s) 446, 541, 997
Hpy166II GTNNAC 2 cut(s) 14, 775
Hpy188I TCNGA 4 cut(s) 139, 307, 727, 1117
Hpy188III TCNNGA 3 cut(s) 177, 202, 433
Hpy8I GTNNAC 2 cut(s) 14, 775
Hpy99I CGWCG 1 cut(s) 737
HpyAV CCTTC 4 cut(s) 418, 732, 787, 1136
HpyCH4III ACNGT 3 cut(s) 61, 349, 1157
HpyCH4V TGCA 4 cut(s) 332, 659, 1010, 1073
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 329, 338, 880, 1067
HpyF3I CTNAG 3 cut(s) 136, 803, 1114
Hsp92II CATG 6 cut(s) 193, 320, 899, 924, 935, 1061
HspAI GCGC 1 cut(s) 170
KpnI GGTACC 1 cut(s) 627
Ksp22I TGATCA 1 cut(s) 987
Kzo9I GATC 2 cut(s) 302, 987
LmnI GCTCC 2 cut(s) 634, 824
Lsp1109I GCAGC 3 cut(s) 170, 575, 1057
LweI GCATC 3 cut(s) 350, 734, 892
MaeI CTAG 4 cut(s) 251, 263, 338, 813
MaeIII GTNAC 6 cut(s) 86, 267, 376, 1003, 1022, 1145
MalI GATC 2 cut(s) 304, 989
MboI GATC 2 cut(s) 302, 987
MboII GAAGA 3 cut(s) 458, 493, 587
MfeI CAATTG 2 cut(s) 297, 750
MflI RGATCY 1 cut(s) 302
MhlI GDGCHC 2 cut(s) 876, 946
MlsI TGGCCA 2 cut(s) 404, 537
MluCI AATT 9 cut(s) 21, 101, 111, 118, 297, 556, 615, 750, 949
MluNI TGGCCA 2 cut(s) 404, 537
MlyI GAGTC 1 cut(s) 1062
Mox20I TGGCCA 2 cut(s) 404, 537
MroXI GAANNNNTTC 2 cut(s) 428, 450
MscI TGGCCA 2 cut(s) 404, 537
MseI TTAA 2 cut(s) 44, 672
MslI CAYNNNNRTG 3 cut(s) 742, 845, 936
Msp20I TGGCCA 2 cut(s) 404, 537
MspI CCGG 1 cut(s) 167
MspR9I CCNGG 1 cut(s) 497
MunI CAATTG 2 cut(s) 297, 750
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 1 cut(s) 497
MwoI GCNNNNNNNGC 5 cut(s) 167, 329, 338, 880, 1067
NdeII GATC 2 cut(s) 302, 987
NlaIII CATG 6 cut(s) 193, 320, 899, 924, 935, 1061
NlaIV GGNNCC 6 cut(s) 181, 625, 681, 820, 835, 943
NmuCI GTSAC 5 cut(s) 86, 376, 1003, 1022, 1145
NspV TTCGAA 1 cut(s) 131
PctI GAATGC 1 cut(s) 428
PdmI GAANNNNTTC 2 cut(s) 428, 450
PfeI GAWTC 3 cut(s) 128, 384, 466
PkrI GCNGC 3 cut(s) 160, 565, 1072
PleI GAGTC 1 cut(s) 1061
PpsI GAGTC 1 cut(s) 1061
PsiI TTATAA 2 cut(s) 278, 312
Psp124BI GAGCTC 1 cut(s) 876
Psp6I CCWGG 1 cut(s) 495
PspEI GGTNACC 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 1044
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 6 cut(s) 181, 625, 681, 820, 835, 943
PspPI GGNCC 1 cut(s) 179
PstNI CAGNNNCTG 1 cut(s) 1070
PsuI RGATCY 1 cut(s) 302
RsaI GTAC 4 cut(s) 15, 625, 669, 913
RsaNI GTAC 4 cut(s) 14, 624, 668, 912
RseI CAYNNNNRTG 3 cut(s) 742, 845, 936
SacI GAGCTC 1 cut(s) 876
SaqAI TTAA 2 cut(s) 44, 672
SatI GCNGC 3 cut(s) 159, 564, 1071
Sau3AI GATC 2 cut(s) 302, 987
Sau96I GGNCC 1 cut(s) 179
SchI GAGTC 1 cut(s) 1062
ScrFI CCNGG 1 cut(s) 497
SduI GDGCHC 2 cut(s) 876, 946
SfaNI GCATC 3 cut(s) 350, 734, 892
SfcI CTRYAG 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 131
SinI GGWCC 1 cut(s) 179
SmiMI CAYNNNNRTG 3 cut(s) 742, 845, 936
SmlI CTYRAG 4 cut(s) 255, 600, 869, 960
SmoI CTYRAG 4 cut(s) 255, 600, 869, 960
Sse9I AATT 9 cut(s) 21, 101, 111, 118, 297, 556, 615, 750, 949
SsiI CCGC 1 cut(s) 710
SspMI CTAG 4 cut(s) 251, 263, 338, 813
SstI GAGCTC 1 cut(s) 876
StyD4I CCNGG 1 cut(s) 495
TaaI ACNGT 3 cut(s) 61, 349, 1157
TaqI TCGA 4 cut(s) 105, 131, 464, 732
TasI AATT 9 cut(s) 21, 101, 111, 118, 297, 556, 615, 750, 949
TfiI GAWTC 3 cut(s) 128, 384, 466
Tru1I TTAA 2 cut(s) 44, 672
Tru9I TTAA 2 cut(s) 44, 672
TscAI CASTG 4 cut(s) 66, 352, 385, 690
TseFI GTSAC 5 cut(s) 86, 376, 1003, 1022, 1145
TseI GCWGC 3 cut(s) 158, 563, 1070
Tsp45I GTSAC 5 cut(s) 86, 376, 1003, 1022, 1145
TspDTI ATGAA 1 cut(s) 437
TspRI CASTG 4 cut(s) 66, 352, 385, 690
VpaK11BI GGWCC 1 cut(s) 179
XapI RAATTY 3 cut(s) 21, 101, 111
XmnI GAANNNNTTC 2 cut(s) 428, 450
XspI CTAG 4 cut(s) 251, 263, 338, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.