pycom07g14220

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0019822
Physical Location & Seq
Reverse (-)
16173038 .. 16174568
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g14220.3

Sequence Viewer

Length: 579 bp
ATGACATACTTCAAGAGACCTGTTGGTCGGGCTACAGATGGGAGGCTCATTGTTGATTTTCTAGGGTTACCATTTATAAGTCCATATCTGAAATCAATTGGATCTGATTATAAACATGGGGCAAACTTTGCAACGCTAGCATCCACTGTGCTTCTGCCGAACACTTCCTTATTTGTCACTGTAACCAGCTCATTCTCTCTGGCAATCCAGCTCAACCAAATGAAGGCATTCAAGACCCAAGTTGAAGAGTTTCACCATTCCTCGGATCAACAAGTTTTTGTTAGTTCTGATGACTGTGATTGTTGCTGCTTAGAACAGAAGAGACTTCCTTCCCTGGATATTTTTGGGAAATCACTCTACACATTCCATATTGGCCAAAACGATTTCACCTCCAATTTGGCAGCCATCGGTGTAGGTGGAGTGAAGCAATATCTTCCTCTGGTCTCCCAAATTGCTGGTACCATCAAGGAGCTTTATACATTAGGAGGGCGTGCATTTCTGGTTCTTAATCTGGCACCAGTGGGTTGTTATCCATCATTTTTGGCGGGGCTTCCTCCTTCAGAACTCGACGCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

193

Amino Acids

20.93

Weight (kDa)

6.39

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019822)

Species Orthologous Gene IDs
pyrus_communis pycom07g14220
rosa_chinensis RchiOBHm_Chr6g0265931
rosa_laevigata RLG00000014145
rosa_roxburghii Rroxscaffold_7G00202190
rosa_samantha Rh6BG143100 Rh6DG127400
rosa_wichuraiana Rw6G012360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 77, 111
AasI GACNNNNNNGTC 1 cut(s) 24
Acc65I GGTACC 1 cut(s) 458
AccB1I GGYRCC 2 cut(s) 458, 514
AciI CCGC 1 cut(s) 545
AclWI GGATC 2 cut(s) 109, 273
AcoI YGGCCR 1 cut(s) 373
AcuI CTGAAG 1 cut(s) 543
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 2 cut(s) 223, 262
AgsI TTSAA 3 cut(s) 13, 232, 245
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 3 cut(s) 189, 211, 472
AluI AGCT 3 cut(s) 189, 211, 472
Alw26I GTCTC 3 cut(s) 10, 316, 448
AlwI GGATC 2 cut(s) 109, 273
AoxI GGCC 1 cut(s) 373
ApeKI GCWGC 2 cut(s) 306, 401
Asp700I GAANNNNTTC 2 cut(s) 227, 249
Asp718I GGTACC 1 cut(s) 458
AsuHPI GGTGA 2 cut(s) 245, 379
AsuNHI GCTAGC 1 cut(s) 136
BalI TGGCCA 1 cut(s) 375
BanI GGYRCC 2 cut(s) 458, 514
BbvI GCAGC 2 cut(s) 293, 413
BccI CCATC 4 cut(s) 32, 413, 470, 541
BciT130I CCWGG 1 cut(s) 335
BcoDI GTCTC 3 cut(s) 10, 316, 448
BfaI CTAG 2 cut(s) 62, 137
BfmI CTRYAG 1 cut(s) 33
BisI GCNGC 2 cut(s) 307, 402
BlsI GCNGC 2 cut(s) 308, 403
Bme1390I CCNGG 1 cut(s) 335
BmiI GGNNCC 2 cut(s) 460, 516
BmrFI CCNGG 1 cut(s) 335
BmsI GCATC 1 cut(s) 149
BmtI GCTAGC 1 cut(s) 140
BsaI GGTCTC 2 cut(s) 10, 448
BsaJI CCNNGG 2 cut(s) 261, 333
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 262
Bse1I ACTGG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 2 cut(s) 261, 333
BseGI GGATG 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 223, 262
BseNI ACTGG 1 cut(s) 518
BseXI GCAGC 2 cut(s) 293, 413
BshFI GGCC 1 cut(s) 375
BshNI GGYRCC 2 cut(s) 458, 514
BslI CCNNNNNNNGG 2 cut(s) 223, 262
BsmAI GTCTC 3 cut(s) 10, 316, 448
BsmI GAATGC 1 cut(s) 227
BsnI GGCC 1 cut(s) 375
Bso31I GGTCTC 2 cut(s) 10, 448
Bsp143I GATC 2 cut(s) 101, 265
BspACI CCGC 1 cut(s) 545
BspANI GGCC 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 460, 516
BspOI GCTAGC 1 cut(s) 140
BspPI GGATC 2 cut(s) 109, 273
BspT107I GGYRCC 2 cut(s) 458, 514
BspTNI GGTCTC 2 cut(s) 10, 448
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 261, 333
BssMI GATC 2 cut(s) 101, 265
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 3 cut(s) 148, 181, 296
Bst6I CTCTTC 2 cut(s) 240, 314
BstAPI GCANNNNNTGC 1 cut(s) 128
BstC8I GCNNGC 2 cut(s) 138, 492
BstDEI CTNAG 1 cut(s) 310
BstEII GGTNACC 1 cut(s) 66
BstF5I GGATG 1 cut(s) 140
BstKTI GATC 2 cut(s) 104, 268
BstMAI GTCTC 3 cut(s) 10, 316, 448
BstMBI GATC 2 cut(s) 101, 265
BstMWI GCNNNNNNNGC 2 cut(s) 128, 137
BstNI CCWGG 1 cut(s) 335
BstPI GGTNACC 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 333
BstSFI CTRYAG 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 293, 413
BstX2I RGATCY 1 cut(s) 101
BstXI CCANNNNNNTGG 1 cut(s) 455
BstYI RGATCY 1 cut(s) 101
BsuRI GGCC 1 cut(s) 375
BtsCI GGATG 1 cut(s) 140
BtsIMutI CAGTG 3 cut(s) 144, 177, 525
Cac8I GCNNGC 2 cut(s) 138, 492
Csp6I GTAC 1 cut(s) 459
CviAII CATG 1 cut(s) 116
CviJI RGCY 8 cut(s) 32, 46, 189, 211, 375, 404, 472, 550
CviKI_1 RGCY 8 cut(s) 32, 46, 189, 211, 375, 404, 472, 550
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 1 cut(s) 310
DpnI GATC 2 cut(s) 103, 267
DpnII GATC 2 cut(s) 101, 265
DrdI GACNNNNNNGTC 1 cut(s) 24
DseDI GACNNNNNNGTC 1 cut(s) 24
EaeI YGGCCR 1 cut(s) 373
Eam1104I CTCTTC 2 cut(s) 240, 314
EarI CTCTTC 2 cut(s) 240, 314
Eco31I GGTCTC 2 cut(s) 10, 448
Eco57I CTGAAG 1 cut(s) 543
Eco91I GGTNACC 1 cut(s) 66
EcoO65I GGTNACC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 333
FaeI CATG 1 cut(s) 119
FaiI YATR 7 cut(s) 7, 77, 85, 111, 117, 369, 477
FatI CATG 1 cut(s) 115
FauI CCCGC 1 cut(s) 538
Fnu4HI GCNGC 2 cut(s) 307, 402
FokI GGATG 1 cut(s) 127
Fsp4HI GCNGC 2 cut(s) 307, 402
FspBI CTAG 2 cut(s) 62, 137
GluI GCNGC 2 cut(s) 307, 402
HaeIII GGCC 1 cut(s) 375
Hin1II CATG 1 cut(s) 119
HphI GGTGA 2 cut(s) 245, 379
Hpy188I TCNGA 5 cut(s) 90, 106, 265, 289, 562
Hpy188III TCNNGA 2 cut(s) 13, 232
Hpy99I CGWCG 1 cut(s) 572
HpyAV CCTTC 3 cut(s) 217, 339, 567
HpyCH4III ACNGT 3 cut(s) 148, 181, 296
HpyCH4V TGCA 2 cut(s) 131, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 137
HpyF3I CTNAG 1 cut(s) 310
Hsp92II CATG 1 cut(s) 119
KpnI GGTACC 1 cut(s) 462
Kzo9I GATC 2 cut(s) 101, 265
LmnI GCTCC 1 cut(s) 469
Lsp1109I GCAGC 2 cut(s) 293, 413
LweI GCATC 1 cut(s) 149
MaeI CTAG 2 cut(s) 62, 137
MaeIII GTNAC 3 cut(s) 66, 175, 181
MalI GATC 2 cut(s) 103, 267
MboI GATC 2 cut(s) 101, 265
MboII GAAGA 3 cut(s) 257, 331, 425
MfeI CAATTG 1 cut(s) 96
MflI RGATCY 1 cut(s) 101
MlsI TGGCCA 1 cut(s) 375
MluCI AATT 3 cut(s) 96, 394, 450
MluNI TGGCCA 1 cut(s) 375
MnlI CCTC 6 cut(s) 36, 271, 400, 447, 479, 564
Mox20I TGGCCA 1 cut(s) 375
MroXI GAANNNNTTC 2 cut(s) 227, 249
MscI TGGCCA 1 cut(s) 375
MseI TTAA 1 cut(s) 507
Msp20I TGGCCA 1 cut(s) 375
MspR9I CCNGG 1 cut(s) 335
MunI CAATTG 1 cut(s) 96
Mva1269I GAATGC 1 cut(s) 227
MvaI CCWGG 1 cut(s) 335
MwoI GCNNNNNNNGC 2 cut(s) 128, 137
NdeII GATC 2 cut(s) 101, 265
NheI GCTAGC 1 cut(s) 136
NlaIII CATG 1 cut(s) 119
NlaIV GGNNCC 2 cut(s) 460, 516
NmuCI GTSAC 1 cut(s) 175
PctI GAATGC 1 cut(s) 227
PdmI GAANNNNTTC 2 cut(s) 227, 249
PkrI GCNGC 2 cut(s) 308, 403
PsiI TTATAA 2 cut(s) 77, 111
Psp6I CCWGG 1 cut(s) 333
PspEI GGTNACC 1 cut(s) 66
PspGI CCWGG 1 cut(s) 333
PspN4I GGNNCC 2 cut(s) 460, 516
PsuI RGATCY 1 cut(s) 101
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 2 cut(s) 307, 402
Sau3AI GATC 2 cut(s) 101, 265
ScrFI CCNGG 1 cut(s) 335
SetI ASST 6 cut(s) 22, 191, 213, 392, 418, 474
SfaNI GCATC 1 cut(s) 149
SfcI CTRYAG 1 cut(s) 33
Sse9I AATT 3 cut(s) 96, 394, 450
SsiI CCGC 1 cut(s) 545
SspMI CTAG 2 cut(s) 62, 137
StyD4I CCNGG 1 cut(s) 333
TaaI ACNGT 3 cut(s) 148, 181, 296
TaqI TCGA 1 cut(s) 567
TasI AATT 3 cut(s) 96, 394, 450
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 3 cut(s) 151, 184, 525
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 2 cut(s) 306, 401
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 1 cut(s) 236
TspRI CASTG 3 cut(s) 151, 184, 525
XmnI GAANNNNTTC 2 cut(s) 227, 249
XspI CTAG 2 cut(s) 62, 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.