Rroxscaffold_7G00202190

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0019822
Physical Location & Seq
Reverse (-)
50126710 .. 50127293
584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00202190.1

Sequence Viewer

Length: 462 bp
ATGGCAATACTCATTACTATTATTCGTCATCATGGCTACGTACTCTTACCAAATGATCATCCTCCTCGTGTTAAGTGGAGATTAAGGGCATTTTTAGCCTTTGCTGCATTTTCACAGGGGATGCACGCAATCAAAAGACTCCGGTTTTACTTTCCTAGGGAGTCGAGCCGGTCGCCTGATCGTGAATTTCTTGGTGCAGCTGATGGTCTTGGCTTCCCAATTCTGCCGTCGGTTCACTTCGATTTCAGATATGGCGCAAACTTTGCAACCATGGGATCAGCTGTACTCGTGCCAAACAGTATCTCTGCACTAGTAAACCCCCCAACCACAAGCCCACTCACTCCAGCCTTTCAGCTTGGACAGTTTAAGGCTTCCCAAAACCGAGTGCTTCATCAATTGCAATACAATAATTCCAAGCCAGCTGGTGAGGGTAAATATGTATACATACATTCGCTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

153

Amino Acids

17.15

Weight (kDa)

10.21

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019822)

Species Orthologous Gene IDs
pyrus_communis pycom07g14220
rosa_chinensis RchiOBHm_Chr6g0265931
rosa_laevigata RLG00000014145
rosa_roxburghii Rroxscaffold_7G00202190
rosa_samantha Rh6BG143100 Rh6DG127400
rosa_wichuraiana Rw6G012360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 441
AclWI GGATC 1 cut(s) 283
AcsI RAATTY 1 cut(s) 185
AfaI GTAC 2 cut(s) 42, 285
AhlI ACTAGT 1 cut(s) 310
AluBI AGCT 4 cut(s) 200, 281, 355, 422
AluI AGCT 4 cut(s) 200, 281, 355, 422
AlwI GGATC 1 cut(s) 283
ApeKI GCWGC 2 cut(s) 104, 197
ApoI RAATTY 1 cut(s) 185
AspA2I CCTAGG 1 cut(s) 155
AspLEI GCGC 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 437
AvrII CCTAGG 1 cut(s) 155
BauI CACGAG 2 cut(s) 66, 287
BbvI GCAGC 2 cut(s) 91, 209
BccI CCATC 1 cut(s) 197
BceAI ACGGC 1 cut(s) 211
BclI TGATCA 1 cut(s) 55
BcuI ACTAGT 1 cut(s) 310
BfaI CTAG 2 cut(s) 156, 311
BisI GCNGC 2 cut(s) 105, 198
BlnI CCTAGG 1 cut(s) 155
BlsI GCNGC 2 cut(s) 106, 199
BmsI GCATC 1 cut(s) 111
BpmI CTGGAG 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 155, 270
BsaWI WCCGGW 1 cut(s) 141
Bse118I RCCGGY 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 155, 270
BseGI GGATG 2 cut(s) 58, 126
BseRI GAGGAG 1 cut(s) 54
BseXI GCAGC 2 cut(s) 91, 209
BsgI GTGCAG 2 cut(s) 216, 291
Bsh1285I CGRYCG 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 173
BsiSI CCGG 2 cut(s) 142, 169
Bsp143I GATC 3 cut(s) 55, 178, 275
Bsp19I CCATGG 1 cut(s) 270
BspPI GGATC 1 cut(s) 283
BsrFI RCCGGY 1 cut(s) 168
BssAI RCCGGY 1 cut(s) 168
BssECI CCNNGG 2 cut(s) 155, 270
BssMI GATC 3 cut(s) 55, 178, 275
BssNAI GTATAC 1 cut(s) 442
BssSI CACGAG 2 cut(s) 66, 287
BssT1I CCWWGG 2 cut(s) 155, 270
Bst1107I GTATAC 1 cut(s) 442
Bst2BI CACGAG 2 cut(s) 66, 287
Bst4CI ACNGT 2 cut(s) 299, 363
BstAPI GCANNNNNTGC 1 cut(s) 263
BstBAI YACGTR 1 cut(s) 40
BstC8I GCNNGC 2 cut(s) 126, 420
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 2 cut(s) 58, 126
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 3 cut(s) 58, 181, 278
BstMBI GATC 3 cut(s) 55, 178, 275
BstMCI CGRYCG 1 cut(s) 173
BstMWI GCNNNNNNNGC 3 cut(s) 95, 104, 263
BstSNI TACGTA 1 cut(s) 40
BstV1I GCAGC 2 cut(s) 91, 209
BstZ17I GTATAC 1 cut(s) 442
BtgI CCRYGG 1 cut(s) 270
BtsCI GGATG 2 cut(s) 58, 126
Cac8I GCNNGC 2 cut(s) 126, 420
CfoI GCGC 1 cut(s) 257
Cfr10I RCCGGY 1 cut(s) 168
Csp6I GTAC 2 cut(s) 41, 284
CviAII CATG 2 cut(s) 32, 271
CviQI GTAC 2 cut(s) 41, 284
DpnI GATC 3 cut(s) 57, 180, 277
DpnII GATC 3 cut(s) 55, 178, 275
Eco105I TACGTA 1 cut(s) 40
Eco130I CCWWGG 2 cut(s) 155, 270
EcoT14I CCWWGG 2 cut(s) 155, 270
ErhI CCWWGG 2 cut(s) 155, 270
FaeI CATG 2 cut(s) 35, 274
FaiI YATR 6 cut(s) 33, 252, 272, 438, 442, 446
FatI CATG 2 cut(s) 31, 270
FbaI TGATCA 1 cut(s) 55
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 2 cut(s) 105, 198
FokI GGATG 2 cut(s) 45, 133
Fsp4HI GCNGC 2 cut(s) 105, 198
FspBI CTAG 2 cut(s) 156, 311
GlaI GCGC 1 cut(s) 256
GluI GCNGC 2 cut(s) 105, 198
GsuI CTGGAG 1 cut(s) 327
HapII CCGG 2 cut(s) 142, 169
HhaI GCGC 1 cut(s) 257
Hin1II CATG 2 cut(s) 35, 274
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HinfI GANTC 2 cut(s) 138, 161
HpaII CCGG 2 cut(s) 142, 169
HphI GGTGA 1 cut(s) 437
Hpy166II GTNNAC 3 cut(s) 235, 316, 442
Hpy188I TCNGA 1 cut(s) 248
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 3 cut(s) 235, 316, 442
Hpy99I CGWCG 1 cut(s) 232
HpyCH4III ACNGT 2 cut(s) 299, 363
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 6 cut(s) 107, 124, 197, 266, 308, 400
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 104, 263
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 2 cut(s) 35, 274
HspAI GCGC 1 cut(s) 255
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 3 cut(s) 55, 178, 275
LpnPI CCDG 7 cut(s) 101, 155, 182, 189, 357, 408, 432
Lsp1109I GCAGC 2 cut(s) 91, 209
LweI GCATC 1 cut(s) 111
MaeI CTAG 2 cut(s) 156, 311
MaeII ACGT 1 cut(s) 39
MalI GATC 3 cut(s) 57, 180, 277
MboI GATC 3 cut(s) 55, 178, 275
MfeI CAATTG 1 cut(s) 395
MluCI AATT 4 cut(s) 185, 219, 395, 409
MlyI GAGTC 2 cut(s) 132, 170
MnlI CCTC 3 cut(s) 72, 75, 421
MseI TTAA 3 cut(s) 72, 83, 366
MspA1I CMGCKG 3 cut(s) 200, 281, 422
MspI CCGG 2 cut(s) 142, 169
MunI CAATTG 1 cut(s) 395
MwoI GCNNNNNNNGC 3 cut(s) 95, 104, 263
NcoI CCATGG 1 cut(s) 270
NdeII GATC 3 cut(s) 55, 178, 275
NlaIII CATG 2 cut(s) 35, 274
PkrI GCNGC 2 cut(s) 106, 199
PleI GAGTC 2 cut(s) 132, 169
PpsI GAGTC 2 cut(s) 132, 169
Ppu21I YACGTR 1 cut(s) 40
PvuII CAGCTG 3 cut(s) 200, 281, 422
RsaI GTAC 2 cut(s) 42, 285
RsaNI GTAC 2 cut(s) 41, 284
SaqAI TTAA 3 cut(s) 72, 83, 366
SatI GCNGC 2 cut(s) 105, 198
Sau3AI GATC 3 cut(s) 55, 178, 275
SchI GAGTC 2 cut(s) 132, 170
SetI ASST 5 cut(s) 42, 202, 283, 357, 424
SfaNI GCATC 1 cut(s) 111
SnaBI TACGTA 1 cut(s) 40
SpeI ACTAGT 1 cut(s) 310
Sse9I AATT 4 cut(s) 185, 219, 395, 409
SspMI CTAG 2 cut(s) 156, 311
StyI CCWWGG 2 cut(s) 155, 270
TaaI ACNGT 2 cut(s) 299, 363
TaiI ACGT 1 cut(s) 42
TaqI TCGA 2 cut(s) 164, 240
TasI AATT 4 cut(s) 185, 219, 395, 409
TatI WGTACW 1 cut(s) 283
Tru1I TTAA 3 cut(s) 72, 83, 366
Tru9I TTAA 3 cut(s) 72, 83, 366
TseI GCWGC 2 cut(s) 104, 197
TspDTI ATGAA 1 cut(s) 380
XapI RAATTY 1 cut(s) 185
XmaJI CCTAGG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 441
XspI CTAG 2 cut(s) 156, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.