RchiOBHm_Chr6g0265931

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0019822
Physical Location & Seq
Forward (+)
21951129 .. 21951784
656 bp
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UTR
Exon/CDS
Intron
PRQ23848

Sequence Viewer

Length: 486 bp
ATGGCAATACTCAGTACTATTATTCGTCATCATGGCTACGTACTCTTACCAAATTATCATCCTCGTCGTGTTAAGTGGAGATTAAGGGCATTTTTAGCCTTGGCTACTCGCACTCCGAAACAGGGGTTATTTTGCTGCATTTTCCACAGGGGATGCACGCAATCAAAAGACTCCGGTTTTACTTTCCTAGGGAGTCGAGCCGGTCGTGTCCCTGATGTGGATTTCTTGGCTGATGGTCTTGGCTTCCCATTTCTGCCGTCGGTTCACTTCGATTTCAGATATGGCGCAAACTTTGCAACAATGGGATCAGCTGTTCTCGTGCCAAACAGTATCTCTGCACCAGTAAACCCCCCAACCACAAGCCCACTCACTCCAGACTTTCAGCTCGGACAGTTTAAGGCTTCCCAAAACCGAGTGCTTCATCAATTGCAATACAATAATTCCAAGCCAGCTGGTGAATATGTATACATACATTCGCTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

161

Amino Acids

17.97

Weight (kDa)

9.81

Isoelectric Point (pI)

35.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019822)

Species Orthologous Gene IDs
pyrus_communis pycom07g14220
rosa_chinensis RchiOBHm_Chr6g0265931
rosa_laevigata RLG00000014145
rosa_roxburghii Rroxscaffold_7G00202190
rosa_samantha Rh6BG143100 Rh6DG127400
rosa_wichuraiana Rw6G012360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 465
AclWI GGATC 1 cut(s) 313
AfaI GTAC 2 cut(s) 16, 42
AfiI CCNNNNNNNGG 2 cut(s) 122, 217
AloI GAACNNNNNNTCC 2 cut(s) 297, 329
AluBI AGCT 3 cut(s) 311, 385, 452
AluI AGCT 3 cut(s) 311, 385, 452
AlwI GGATC 1 cut(s) 313
ApeKI GCWGC 1 cut(s) 135
AspA2I CCTAGG 1 cut(s) 187
AspLEI GCGC 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 467
AvrII CCTAGG 1 cut(s) 187
BauI CACGAG 1 cut(s) 317
BbvI GCAGC 1 cut(s) 122
BccI CCATC 1 cut(s) 227
BceAI ACGGC 1 cut(s) 241
BfaI CTAG 1 cut(s) 188
BisI GCNGC 1 cut(s) 136
BlnI CCTAGG 1 cut(s) 187
BlsI GCNGC 1 cut(s) 137
BmcAI AGTACT 1 cut(s) 16
BmsI GCATC 1 cut(s) 143
BpmI CTGGAG 1 cut(s) 357
BsaAI YACGTR 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 99, 187
BsaWI WCCGGW 1 cut(s) 173
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 217
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 341
BseDI CCNNGG 2 cut(s) 99, 187
BseGI GGATG 2 cut(s) 58, 158
BseLI CCNNNNNNNGG 2 cut(s) 122, 217
BseMII CTCAG 1 cut(s) 25
BseNI ACTGG 1 cut(s) 341
BseXI GCAGC 1 cut(s) 122
BsgI GTGCAG 1 cut(s) 321
Bsh1285I CGRYCG 1 cut(s) 205
BsiEI CGRYCG 1 cut(s) 205
BsiSI CCGG 2 cut(s) 174, 201
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 2 cut(s) 122, 217
BsmFI GGGAC 1 cut(s) 194
Bsp143I GATC 1 cut(s) 305
BspCNI CTCAG 1 cut(s) 24
BspPI GGATC 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 1 cut(s) 341
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 2 cut(s) 99, 187
BssMI GATC 1 cut(s) 305
BssNAI GTATAC 1 cut(s) 466
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 2 cut(s) 99, 187
Bst1107I GTATAC 1 cut(s) 466
Bst2BI CACGAG 1 cut(s) 317
Bst4CI ACNGT 2 cut(s) 329, 393
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 40
BstC8I GCNNGC 2 cut(s) 158, 450
BstDEI CTNAG 1 cut(s) 11
BstF5I GGATG 2 cut(s) 58, 158
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 1 cut(s) 308
BstMBI GATC 1 cut(s) 305
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 2 cut(s) 95, 293
BstSNI TACGTA 1 cut(s) 40
BstV1I GCAGC 1 cut(s) 122
BstZ17I GTATAC 1 cut(s) 466
BtsCI GGATG 2 cut(s) 58, 158
Cac8I GCNNGC 2 cut(s) 158, 450
CfoI GCGC 1 cut(s) 287
Cfr10I RCCGGY 1 cut(s) 200
Csp6I GTAC 2 cut(s) 15, 41
CviAII CATG 1 cut(s) 32
CviQI GTAC 2 cut(s) 15, 41
DdeI CTNAG 1 cut(s) 11
DpnI GATC 1 cut(s) 307
DpnII GATC 1 cut(s) 305
Eco105I TACGTA 1 cut(s) 40
Eco130I CCWWGG 2 cut(s) 99, 187
EcoT14I CCWWGG 2 cut(s) 99, 187
ErhI CCWWGG 2 cut(s) 99, 187
FaeI CATG 1 cut(s) 35
FaiI YATR 5 cut(s) 33, 282, 462, 466, 470
FaqI GGGAC 1 cut(s) 194
FatI CATG 1 cut(s) 31
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 1 cut(s) 136
FokI GGATG 2 cut(s) 45, 165
Fsp4HI GCNGC 1 cut(s) 136
FspBI CTAG 1 cut(s) 188
GlaI GCGC 1 cut(s) 286
GluI GCNGC 1 cut(s) 136
GsuI CTGGAG 1 cut(s) 357
HapII CCGG 2 cut(s) 174, 201
HhaI GCGC 1 cut(s) 287
Hin1II CATG 1 cut(s) 35
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HinfI GANTC 2 cut(s) 170, 193
HpaII CCGG 2 cut(s) 174, 201
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 3 cut(s) 265, 346, 466
Hpy188I TCNGA 3 cut(s) 117, 278, 389
Hpy188III TCNNGA 1 cut(s) 374
Hpy8I GTNNAC 3 cut(s) 265, 346, 466
Hpy99I CGWCG 2 cut(s) 69, 262
HpyCH4III ACNGT 2 cut(s) 329, 393
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 5 cut(s) 138, 156, 296, 338, 430
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 293
HpyF3I CTNAG 1 cut(s) 11
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 1 cut(s) 35
HspAI GCGC 1 cut(s) 285
Kzo9I GATC 1 cut(s) 305
LpnPI CCDG 9 cut(s) 107, 133, 187, 214, 225, 354, 387, 438, 462
Lsp1109I GCAGC 1 cut(s) 122
LweI GCATC 1 cut(s) 143
MaeI CTAG 1 cut(s) 188
MaeII ACGT 1 cut(s) 39
MalI GATC 1 cut(s) 307
MboI GATC 1 cut(s) 305
MfeI CAATTG 1 cut(s) 425
MluCI AATT 3 cut(s) 52, 425, 439
MlyI GAGTC 2 cut(s) 164, 202
MnlI CCTC 1 cut(s) 72
MseI TTAA 3 cut(s) 72, 83, 396
MspA1I CMGCKG 2 cut(s) 311, 452
MspI CCGG 2 cut(s) 174, 201
MunI CAATTG 1 cut(s) 425
MwoI GCNNNNNNNGC 2 cut(s) 95, 293
NdeII GATC 1 cut(s) 305
NlaIII CATG 1 cut(s) 35
PcsI WCGNNNNNNNCGW 1 cut(s) 202
PkrI GCNGC 1 cut(s) 137
PleI GAGTC 2 cut(s) 164, 201
PpsI GAGTC 2 cut(s) 164, 201
Ppu21I YACGTR 1 cut(s) 40
PvuII CAGCTG 2 cut(s) 311, 452
RsaI GTAC 2 cut(s) 16, 42
RsaNI GTAC 2 cut(s) 15, 41
SaqAI TTAA 3 cut(s) 72, 83, 396
SatI GCNGC 1 cut(s) 136
Sau3AI GATC 1 cut(s) 305
ScaI AGTACT 1 cut(s) 16
SchI GAGTC 2 cut(s) 164, 202
SetI ASST 4 cut(s) 42, 313, 387, 454
SfaNI GCATC 1 cut(s) 143
SnaBI TACGTA 1 cut(s) 40
Sse9I AATT 3 cut(s) 52, 425, 439
SspMI CTAG 1 cut(s) 188
StyI CCWWGG 2 cut(s) 99, 187
TaaI ACNGT 2 cut(s) 329, 393
TaiI ACGT 1 cut(s) 42
TaqI TCGA 2 cut(s) 196, 270
TasI AATT 3 cut(s) 52, 425, 439
TatI WGTACW 1 cut(s) 14
Tru1I TTAA 3 cut(s) 72, 83, 396
Tru9I TTAA 3 cut(s) 72, 83, 396
TseI GCWGC 1 cut(s) 135
TspDTI ATGAA 1 cut(s) 410
XmaJI CCTAGG 1 cut(s) 187
XmiI GTMKAC 1 cut(s) 465
XspI CTAG 1 cut(s) 188
ZrmI AGTACT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.