pycom07g19590
ERF Family

HAD superfamily, subfamily IIIB (Acid phosphatase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005130
Physical Location & Seq
Reverse (-)
21906738 .. 21907845
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 690 bp
ATGTCTGCCTATGGCCGTCATATGGAGCGGGAGTACTCCGCGCAGAGTCTTTCCAATGAAGGTTCAGAAAGGGCAAGCAGTTATGCCATGGAGACAGGATTCTACATGTCGTCTTTTGCTACGACAATCTTCATTGGCGCACTTATAACTGTTGGGCTCTTATTAATCGCCTTGCTGATTGCACTGACTGTAATGCTGCAGTCTTGCGAAAATAGGAGTCATGGAATCGTTGAGACTCATAACCCGAGTTTCGATTACAATCACTGCCAGATCTTGTCTCTGCATATGGAACTCAACGGTGAAGCAGATCACTTCCCTTCAATGTGCAGGGTTGTTGCCCTTCAGTACATCAAAGAAGGTCAATATGTAAGAGATTTAAATTCTACCATGTCAATGATTCAGAATTATTTCAGCGGCATTACCCCAGCACACGATGGTCTGGACGTGGTCTTGATCGACATAGATGACATTCGTTCTTCAAATCCTCGGCACATTAATTTGCCACTGCCCCAATATGATCAGTATGGTTGTAGTGATTGTGTGGAAGAGGCAAAGCATCTGAAGCACAGGTTTATTCTGAGATTATACATGGAACTTCATGCTAGCGGTTGGCCTTTGATTCTGCTATCAAGAAAGCCGGAGGCGGATCGGAATTCCTTCATAGAGCGATCTTATATCTGCAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

230

Amino Acids

26.06

Weight (kDa)

5.73

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 79 - 217 2.9e-07 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 146
AccBSI CCGCTC 1 cut(s) 28
AccII CGCG 1 cut(s) 41
AciI CCGC 5 cut(s) 28, 39, 414, 606, 644
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 2 cut(s) 379, 652
AcuI CTGAAG 2 cut(s) 326, 581
AfaI GTAC 2 cut(s) 35, 347
AfiI CCNNNNNNNGG 1 cut(s) 22
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 2 cut(s) 321, 480
AjiI CACGTC 1 cut(s) 445
Alw26I GTCTC 3 cut(s) 86, 227, 282
AlwI GGATC 1 cut(s) 654
Ama87I CYCGRG 1 cut(s) 244
AoxI GGCC 2 cut(s) 13, 611
ApeKI GCWGC 1 cut(s) 196
ApoI RAATTY 2 cut(s) 379, 652
AseI ATTAAT 2 cut(s) 164, 495
Asp700I GAANNNNTTC 2 cut(s) 407, 656
AspLEI GCGC 2 cut(s) 43, 140
AsuHPI GGTGA 1 cut(s) 311
AsuNHI GCTAGC 1 cut(s) 602
AvaI CYCGRG 1 cut(s) 244
BanII GRGCYC 1 cut(s) 159
BbvI GCAGC 1 cut(s) 183
BccI CCATC 1 cut(s) 428
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 3 cut(s) 86, 227, 282
BfaI CTAG 1 cut(s) 603
BfmI CTRYAG 2 cut(s) 197, 679
BglII AGATCT 1 cut(s) 270
BisI GCNGC 2 cut(s) 197, 415
BlsI GCNGC 2 cut(s) 198, 416
BmcAI AGTACT 1 cut(s) 35
BmeT110I CYCGRG 1 cut(s) 244
BmgBI CACGTC 1 cut(s) 445
BmsI GCATC 1 cut(s) 565
BmtI GCTAGC 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 258
BsaJI CCNNGG 2 cut(s) 87, 485
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse8I GATNNNNATC 1 cut(s) 258
BseDI CCNNGG 2 cut(s) 87, 485
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMII CTCAG 1 cut(s) 569
BseXI GCAGC 1 cut(s) 183
BseYI CCCAGC 1 cut(s) 424
BsgI GTGCAG 1 cut(s) 346
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 2 cut(s) 15, 613
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 1 cut(s) 638
BslI CCNNNNNNNGG 1 cut(s) 22
BsmAI GTCTC 3 cut(s) 86, 227, 282
BsnI GGCC 2 cut(s) 15, 613
BsoBI CYCGRG 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 6 cut(s) 270, 307, 453, 517, 646, 668
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 5 cut(s) 28, 39, 414, 606, 644
BspANI GGCC 2 cut(s) 15, 613
BspCNI CTCAG 1 cut(s) 570
BspFNI CGCG 1 cut(s) 41
BspMAI CTGCAG 2 cut(s) 201, 683
BspOI GCTAGC 1 cut(s) 606
BspPI GGATC 1 cut(s) 654
BsrBI CCGCTC 1 cut(s) 28
BssECI CCNNGG 2 cut(s) 87, 485
BssMI GATC 6 cut(s) 270, 307, 453, 517, 646, 668
BssT1I CCWWGG 1 cut(s) 87
Bst4CI ACNGT 3 cut(s) 151, 190, 299
Bst6I CTCTTC 1 cut(s) 540
BstC8I GCNNGC 2 cut(s) 76, 604
BstDEI CTNAG 1 cut(s) 578
BstDSI CCRYGG 1 cut(s) 87
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 2 cut(s) 43, 140
BstKTI GATC 6 cut(s) 273, 310, 456, 520, 649, 671
BstMAI GTCTC 3 cut(s) 86, 227, 282
BstMBI GATC 6 cut(s) 270, 307, 453, 517, 646, 668
BstMWI GCNNNNNNNGC 1 cut(s) 562
BstNSI RCATGY 1 cut(s) 109
BstSFI CTRYAG 2 cut(s) 197, 679
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BsuRI GGCC 2 cut(s) 15, 613
BtgI CCRYGG 1 cut(s) 87
BtrI CACGTC 1 cut(s) 445
BtsI GCAGTG 2 cut(s) 262, 503
BtsIMutI CAGTG 3 cut(s) 182, 262, 503
Cac8I GCNNGC 2 cut(s) 76, 604
CfoI GCGC 2 cut(s) 43, 140
Csp6I GTAC 2 cut(s) 34, 346
CviAII CATG 6 cut(s) 88, 106, 221, 388, 589, 599
CviJI RGCY 4 cut(s) 15, 157, 613, 637
CviKI_1 RGCY 4 cut(s) 15, 157, 613, 637
CviQI GTAC 2 cut(s) 34, 346
DdeI CTNAG 1 cut(s) 578
DpnI GATC 6 cut(s) 272, 309, 455, 519, 648, 670
DpnII GATC 6 cut(s) 270, 307, 453, 517, 646, 668
DraI TTTAAA 1 cut(s) 378
EaeI YGGCCR 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
EciI GGCGGA 1 cut(s) 659
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 159
Eco57I CTGAAG 2 cut(s) 326, 581
Eco88I CYCGRG 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 652
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 6 cut(s) 91, 109, 224, 391, 592, 602
FatI CATG 6 cut(s) 87, 105, 220, 387, 588, 598
FauI CCCGC 1 cut(s) 21
FauNDI CATATG 2 cut(s) 21, 285
FbaI TGATCA 1 cut(s) 517
Fnu4HI GCNGC 2 cut(s) 197, 415
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 2 cut(s) 197, 415
FspBI CTAG 1 cut(s) 603
GlaI GCGC 2 cut(s) 42, 139
GluI GCNGC 2 cut(s) 197, 415
GsaI CCCAGC 1 cut(s) 428
HaeIII GGCC 2 cut(s) 15, 613
HapII CCGG 1 cut(s) 638
HhaI GCGC 2 cut(s) 43, 140
Hin1II CATG 6 cut(s) 91, 109, 224, 391, 592, 602
Hin6I GCGC 2 cut(s) 41, 138
HinP1I GCGC 2 cut(s) 41, 138
HinfI GANTC 7 cut(s) 46, 99, 217, 225, 235, 397, 619
HpaII CCGG 1 cut(s) 638
HphI GGTGA 1 cut(s) 311
Hpy188I TCNGA 5 cut(s) 67, 402, 561, 579, 651
Hpy188III TCNNGA 3 cut(s) 440, 451, 630
HpyAV CCTTC 5 cut(s) 53, 327, 350, 350, 667
HpyCH4III ACNGT 3 cut(s) 151, 190, 299
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 5 cut(s) 182, 199, 283, 327, 681
HpyF10VI GCNNNNNNNGC 1 cut(s) 562
HpyF3I CTNAG 1 cut(s) 578
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 6 cut(s) 91, 109, 224, 391, 592, 602
HspAI GCGC 2 cut(s) 41, 138
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 6 cut(s) 270, 307, 453, 517, 646, 668
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 8 cut(s) 81, 281, 313, 425, 438, 553, 651, 667
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 1 cut(s) 565
MaeI CTAG 1 cut(s) 603
MaeII ACGT 1 cut(s) 444
MalI GATC 6 cut(s) 272, 309, 455, 519, 648, 670
MbiI CCGCTC 1 cut(s) 28
MboI GATC 6 cut(s) 270, 307, 453, 517, 646, 668
MboII GAAGA 3 cut(s) 121, 468, 557
MflI RGATCY 1 cut(s) 270
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 4 cut(s) 379, 403, 496, 652
MlyI GAGTC 3 cut(s) 55, 226, 229
MnlI CCTC 3 cut(s) 495, 541, 634
MroXI GAANNNNTTC 2 cut(s) 407, 656
MseI TTAA 3 cut(s) 164, 377, 495
MslI CAYNNNNRTG 1 cut(s) 392
MspA1I CMGCKG 1 cut(s) 414
MspI CCGG 1 cut(s) 638
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 562
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 2 cut(s) 21, 285
NdeII GATC 6 cut(s) 270, 307, 453, 517, 646, 668
NheI GCTAGC 1 cut(s) 602
NlaIII CATG 6 cut(s) 91, 109, 224, 391, 592, 602
NmeAIII GCCGAG 1 cut(s) 466
NspI RCATGY 1 cut(s) 109
PciI ACATGT 1 cut(s) 105
PdmI GAANNNNTTC 2 cut(s) 407, 656
PfeI GAWTC 4 cut(s) 99, 225, 397, 619
PflFI GACNNNGTC 1 cut(s) 446
PkrI GCNGC 2 cut(s) 198, 416
PleI GAGTC 3 cut(s) 54, 225, 229
PpsI GAGTC 3 cut(s) 54, 225, 229
PscI ACATGT 1 cut(s) 105
PshBI ATTAAT 2 cut(s) 164, 495
PsiI TTATAA 1 cut(s) 146
PspFI CCCAGC 1 cut(s) 424
PstI CTGCAG 2 cut(s) 201, 683
PsuI RGATCY 1 cut(s) 270
PsyI GACNNNGTC 1 cut(s) 446
RsaI GTAC 2 cut(s) 35, 347
RsaNI GTAC 2 cut(s) 34, 346
RseI CAYNNNNRTG 1 cut(s) 392
SaqAI TTAA 3 cut(s) 164, 377, 495
SatI GCNGC 2 cut(s) 197, 415
Sau3AI GATC 6 cut(s) 270, 307, 453, 517, 646, 668
ScaI AGTACT 1 cut(s) 35
SchI GAGTC 3 cut(s) 55, 226, 229
SduI GDGCHC 1 cut(s) 159
SetI ASST 4 cut(s) 64, 361, 447, 572
SfaNI GCATC 1 cut(s) 565
SfcI CTRYAG 2 cut(s) 197, 679
SmiI ATTTAAAT 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 392
Sse9I AATT 4 cut(s) 379, 403, 496, 652
SsiI CCGC 5 cut(s) 28, 39, 414, 606, 644
SspMI CTAG 1 cut(s) 603
StyI CCWWGG 1 cut(s) 87
SwaI ATTTAAAT 1 cut(s) 378
TaaI ACNGT 3 cut(s) 151, 190, 299
TaiI ACGT 1 cut(s) 447
TaqI TCGA 2 cut(s) 252, 456
TasI AATT 4 cut(s) 379, 403, 496, 652
TatI WGTACW 2 cut(s) 33, 345
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 4 cut(s) 99, 225, 397, 619
Tru1I TTAA 3 cut(s) 164, 377, 495
Tru9I TTAA 3 cut(s) 164, 377, 495
TscAI CASTG 3 cut(s) 189, 269, 510
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 4 cut(s) 72, 121, 587, 649
TspRI CASTG 3 cut(s) 189, 269, 510
Tth111I GACNNNGTC 1 cut(s) 446
VspI ATTAAT 2 cut(s) 164, 495
XapI RAATTY 2 cut(s) 379, 652
XceI RCATGY 1 cut(s) 109
XmnI GAANNNNTTC 2 cut(s) 407, 656
XspI CTAG 1 cut(s) 603
ZrmI AGTACT 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.