pycom07g24610

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007167
Physical Location & Seq
Forward (+)
25252900 .. 25254349
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1062 bp
ATGCTGTCGCTGGTGCCGGACGACGTCGGAAAGGCGACGGTGAAGCTGGGGAAAGCGGCGGTTCAGGACGGCACCTGCATTTGGGAGAATCCAGTTTTTGAGTCAGTGAAGCTCGTTGAGGAAGTAAAAACAGGAAAACTGAAAGAGAAGATTTATCACTTCATTGTTTCAAATGGATCTTCGAAATCAGGTTATCTCGGAGAAGCTTCAATTGATTTTGCAGATATTGTGGCGGAAACTGAACCGTTAAGCATCACACTGCCCCTTAAATTTGCAAACTCTGGTGTGGTTTTACATGTGACAATTGACAGGATTCGGGAAGATGGTGATCAAAGGGAAATCGAAGAGAGTGACGATCCAAGTCTTTCGCACCATAGCAGCGTAGATGGAAGCAACCACCAAAGTTTCAACGAAACGAATAAAGGATATGCCCATCGTGACGCTGGCAGTTCCCTGTCACCCCCTGAAGTACCAACTTCTATGCCTCAAAATGGACATGCGGGTGCCAACGGAAGGAAAACCCATGTGCGTCAAAAATCAAGCTTGGATTGGTCTTCAGATGAAAGCTTATTTGACTCGCCAGACATTGTTGAAGACAAGCTTCCGACAGGAGTGCAAGCCGTTTCAGATGTTGATAAACTCAGAAATGAAATGACTGCTCTGAAGAGGCAGGCAGACCTTTCGGAACTGGAATTGCAGTCTCTTCGGAAACAGATGGCCAAAGAGAGCAATCAAGGACAGAATCTGTCTAGACAGATTGTTTGCCTTAAAGAGGAGAGAGATGCGCTCAAAATGGAATGTGAACAACTTAAGTCCTCGCAGGGACGTGGTAATGGGAAACGGACTTTCAAAACGTTGCAGCCCGAGACTGAGGATACAAGAGCGCAGTTAGAAGCAATGAAGCAAGAGCTTAGTTCTGAAAAGAAAGCAAGAACCAATCTTCGCTCACAGCTCGCGCAGACACAAGACTCAAATTCTGAGTTAGTCCTTGTCGTGAAGGATCGATCGAAGATGCATTGGGAAAGAAAAATAGAGAGATATCTGATCTTTCAAGCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

354

Amino Acids

39.32

Weight (kDa)

5.71

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 13 - 105 6.2e-15 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 83
AatII GACGTC 1 cut(s) 27
Acc36I ACCTGC 1 cut(s) 83
AccB1I GGYRCC 3 cut(s) 13, 71, 503
AccII CGCG 1 cut(s) 956
AciI CCGC 4 cut(s) 56, 59, 233, 500
AclI AACGTT 1 cut(s) 854
AclWI GGATC 3 cut(s) 184, 350, 1008
AcoI YGGCCR 1 cut(s) 717
AcsI RAATTY 2 cut(s) 269, 973
AcuI CTGAAG 3 cut(s) 486, 540, 683
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 1 cut(s) 471
AfiI CCNNNNNNNGG 4 cut(s) 81, 491, 513, 772
AflII CTTAAG 1 cut(s) 809
AflIII ACRYGT 1 cut(s) 295
AgsI TTSAA 6 cut(s) 171, 210, 409, 593, 850, 1052
AjiI CACGTC 1 cut(s) 827
AluBI AGCT 8 cut(s) 46, 112, 206, 543, 567, 601, 910, 952
AluI AGCT 8 cut(s) 46, 112, 206, 543, 567, 601, 910, 952
Alw26I GTCTC 2 cut(s) 705, 860
AlwI GGATC 3 cut(s) 184, 350, 1008
AlwNI CAGNNNCTG 1 cut(s) 745
Ama87I CYCGRG 1 cut(s) 863
AoxI GGCC 1 cut(s) 717
ApeKI GCWGC 2 cut(s) 378, 859
ApoI RAATTY 2 cut(s) 269, 973
ArsI GACNNNNNNTTYG 2 cut(s) 744, 776
AspLEI GCGC 3 cut(s) 787, 886, 958
AsuHPI GGTGA 3 cut(s) 52, 338, 450
AsuII TTCGAA 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 863
BalI TGGCCA 1 cut(s) 719
BanI GGYRCC 3 cut(s) 13, 71, 503
BbsI GAAGAC 2 cut(s) 546, 600
BbvI GCAGC 2 cut(s) 390, 871
BccI CCATC 4 cut(s) 317, 380, 441, 709
BceAI ACGGC 2 cut(s) 85, 605
BcgI CGANNNNNNTGC 6 cut(s) 595, 629, 663, 686, 697, 720
BciVI GTATCC 1 cut(s) 868
BclI TGATCA 1 cut(s) 328
BcoDI GTCTC 2 cut(s) 705, 860
BfaI CTAG 1 cut(s) 750
BfrI CTTAAG 1 cut(s) 809
BfuAI ACCTGC 1 cut(s) 83
BfuI GTATCC 1 cut(s) 868
BisI GCNGC 3 cut(s) 57, 379, 860
BlsI GCNGC 3 cut(s) 58, 380, 861
BmeT110I CYCGRG 1 cut(s) 863
BmgBI CACGTC 1 cut(s) 827
BmiI GGNNCC 3 cut(s) 15, 73, 505
BmsI GCATC 3 cut(s) 261, 772, 1002
BpiI GAAGAC 2 cut(s) 546, 600
BplI GAGNNNNNCTC 2 cut(s) 771, 803
Bpu14I TTCGAA 1 cut(s) 182
Bsa29I ATCGAT 1 cut(s) 1003
BsaBI GATNNNNATC 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 24
Bsc4I CCNNNNNNNGG 4 cut(s) 81, 491, 513, 772
Bse1I ACTGG 2 cut(s) 92, 693
Bse3DI GCAATG 1 cut(s) 903
Bse8I GATNNNNATC 1 cut(s) 327
BseCI ATCGAT 1 cut(s) 1003
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 4 cut(s) 81, 491, 513, 772
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 3 cut(s) 655, 861, 969
BseNI ACTGG 2 cut(s) 92, 693
BseRI GAGGAG 1 cut(s) 788
BseXI GCAGC 2 cut(s) 390, 871
BseYI CCCAGC 1 cut(s) 46
Bsh1236I CGCG 1 cut(s) 956
Bsh1285I CGRYCG 1 cut(s) 1007
BshFI GGCC 1 cut(s) 719
BshNI GGYRCC 3 cut(s) 13, 71, 503
BshVI ATCGAT 1 cut(s) 1003
BsiEI CGRYCG 1 cut(s) 1007
BsiHKCI CYCGRG 1 cut(s) 863
BsiSI CCGG 1 cut(s) 17
BslFI GGGAC 1 cut(s) 837
BslI CCNNNNNNNGG 4 cut(s) 81, 491, 513, 772
BsmAI GTCTC 2 cut(s) 705, 860
BsmFI GGGAC 1 cut(s) 837
BsnI GGCC 1 cut(s) 719
BsoBI CYCGRG 1 cut(s) 863
Bsp119I TTCGAA 1 cut(s) 182
Bsp143I GATC 6 cut(s) 176, 328, 355, 1000, 1004, 1044
BspACI CCGC 4 cut(s) 56, 59, 233, 500
BspANI GGCC 1 cut(s) 719
BspCNI CTCAG 3 cut(s) 654, 862, 970
BspDI ATCGAT 1 cut(s) 1003
BspFNI CGCG 1 cut(s) 956
BspLI GGNNCC 3 cut(s) 15, 73, 505
BspMI ACCTGC 1 cut(s) 83
BspPI GGATC 3 cut(s) 184, 350, 1008
BspT104I TTCGAA 1 cut(s) 182
BspT107I GGYRCC 3 cut(s) 13, 71, 503
BspTI CTTAAG 1 cut(s) 809
BsrDI GCAATG 1 cut(s) 903
BsrI ACTGG 2 cut(s) 92, 693
BssMI GATC 6 cut(s) 176, 328, 355, 1000, 1004, 1044
BssNI GRCGYC 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 40, 246
Bst6I CTCTTC 3 cut(s) 339, 659, 708
BstACI GRCGYC 1 cut(s) 24
BstAFI CTTAAG 1 cut(s) 809
BstBI TTCGAA 1 cut(s) 182
BstC8I GCNNGC 4 cut(s) 445, 618, 672, 954
BstDEI CTNAG 4 cut(s) 641, 870, 911, 978
BstENI CCTNNNNNAGG 1 cut(s) 770
BstFNI CGCG 1 cut(s) 956
BstHHI GCGC 3 cut(s) 787, 886, 958
BstKTI GATC 6 cut(s) 179, 331, 358, 1003, 1007, 1047
BstMAI GTCTC 2 cut(s) 705, 860
BstMBI GATC 6 cut(s) 176, 328, 355, 1000, 1004, 1044
BstMCI CGRYCG 1 cut(s) 1007
BstNSI RCATGY 2 cut(s) 299, 500
BstUI CGCG 1 cut(s) 956
BstV1I GCAGC 2 cut(s) 390, 871
BstV2I GAAGAC 2 cut(s) 546, 600
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
Bsu15I ATCGAT 1 cut(s) 1003
BsuI GTATCC 1 cut(s) 868
BsuRI GGCC 1 cut(s) 719
BsuTUI ATCGAT 1 cut(s) 1003
BtrI CACGTC 1 cut(s) 827
BtsI GCAGTG 1 cut(s) 257
BtsIMutI CAGTG 2 cut(s) 111, 257
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 4 cut(s) 445, 618, 672, 954
CaiI CAGNNNCTG 1 cut(s) 745
CfoI GCGC 3 cut(s) 787, 886, 958
ClaI ATCGAT 1 cut(s) 1003
CseI GACGC 2 cut(s) 449, 518
Csp6I GTAC 1 cut(s) 470
CviAII CATG 3 cut(s) 296, 497, 524
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 4 cut(s) 641, 870, 911, 978
DpnI GATC 6 cut(s) 178, 330, 357, 1002, 1006, 1046
DpnII GATC 6 cut(s) 176, 328, 355, 1000, 1004, 1044
EaeI YGGCCR 1 cut(s) 717
Eam1104I CTCTTC 3 cut(s) 339, 659, 708
EarI CTCTTC 3 cut(s) 339, 659, 708
EciI GGCGGA 1 cut(s) 248
Eco32I GATATC 1 cut(s) 1040
Eco57I CTGAAG 3 cut(s) 486, 540, 683
Eco88I CYCGRG 1 cut(s) 863
EcoNI CCTNNNNNAGG 1 cut(s) 770
EcoRV GATATC 1 cut(s) 1040
EcoT22I ATGCAT 1 cut(s) 1017
FaeI CATG 3 cut(s) 299, 500, 527
FaiI YATR 6 cut(s) 297, 375, 429, 482, 498, 525
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 1 cut(s) 837
FatI CATG 3 cut(s) 295, 496, 523
FauI CCCGC 1 cut(s) 493
FbaI TGATCA 1 cut(s) 328
Fnu4HI GCNGC 3 cut(s) 57, 379, 860
Fsp4HI GCNGC 3 cut(s) 57, 379, 860
FspBI CTAG 1 cut(s) 750
GlaI GCGC 3 cut(s) 786, 885, 957
GluI GCNGC 3 cut(s) 57, 379, 860
GsaI CCCAGC 1 cut(s) 50
HaeIII GGCC 1 cut(s) 719
HapII CCGG 1 cut(s) 17
HgaI GACGC 2 cut(s) 449, 518
HhaI GCGC 3 cut(s) 787, 886, 958
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 3 cut(s) 299, 500, 527
Hin6I GCGC 3 cut(s) 785, 884, 956
HinP1I GCGC 3 cut(s) 785, 884, 956
HindIII AAGCTT 4 cut(s) 204, 541, 565, 599
HinfI GANTC 6 cut(s) 88, 101, 313, 575, 742, 968
HpaII CCGG 1 cut(s) 17
HphI GGTGA 3 cut(s) 52, 338, 450
Hpy166II GTNNAC 1 cut(s) 803
Hpy188III TCNNGA 5 cut(s) 65, 317, 437, 750, 994
Hpy8I GTNNAC 1 cut(s) 803
Hpy99I CGWCG 3 cut(s) 26, 29, 40
HpyAV CCTTC 2 cut(s) 507, 991
HpyCH4III ACNGT 2 cut(s) 40, 246
HpyCH4IV ACGT 3 cut(s) 24, 826, 854
HpyCH4V TGCA 7 cut(s) 78, 221, 275, 616, 697, 859, 1015
HpyF3I CTNAG 4 cut(s) 641, 870, 911, 978
HpySE526I ACGT 3 cut(s) 24, 826, 854
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 3 cut(s) 299, 500, 527
HspAI GCGC 3 cut(s) 785, 884, 956
Ksp22I TGATCA 1 cut(s) 328
Kzo9I GATC 6 cut(s) 176, 328, 355, 1000, 1004, 1044
Lsp1109I GCAGC 2 cut(s) 390, 871
LweI GCATC 3 cut(s) 261, 772, 1002
MaeI CTAG 1 cut(s) 750
MaeII ACGT 3 cut(s) 24, 826, 854
MaeIII GTNAC 4 cut(s) 298, 350, 437, 456
MalI GATC 6 cut(s) 178, 330, 357, 1002, 1006, 1046
MboI GATC 6 cut(s) 176, 328, 355, 1000, 1004, 1044
MfeI CAATTG 2 cut(s) 210, 303
MflI RGATCY 1 cut(s) 176
MlsI TGGCCA 1 cut(s) 719
MluCI AATT 5 cut(s) 210, 269, 303, 692, 973
MluNI TGGCCA 1 cut(s) 719
MlyI GAGTC 3 cut(s) 110, 569, 962
MmeI TCCRAC 2 cut(s) 7, 629
MnlI CCTC 6 cut(s) 112, 495, 660, 766, 826, 865
Mox20I TGGCCA 1 cut(s) 719
Mph1103I ATGCAT 1 cut(s) 1017
MscI TGGCCA 1 cut(s) 719
MseI TTAA 4 cut(s) 248, 267, 768, 810
MslI CAYNNNNRTG 1 cut(s) 501
Msp20I TGGCCA 1 cut(s) 719
MspCI CTTAAG 1 cut(s) 809
MspI CCGG 1 cut(s) 17
MunI CAATTG 2 cut(s) 210, 303
MvnI CGCG 1 cut(s) 956
NdeII GATC 6 cut(s) 176, 328, 355, 1000, 1004, 1044
NlaIII CATG 3 cut(s) 299, 500, 527
NlaIV GGNNCC 3 cut(s) 15, 73, 505
NmuCI GTSAC 4 cut(s) 298, 350, 437, 456
NsiI ATGCAT 1 cut(s) 1017
NspI RCATGY 2 cut(s) 299, 500
NspV TTCGAA 1 cut(s) 182
PaqCI CACCTGC 1 cut(s) 83
PciI ACATGT 1 cut(s) 295
PfeI GAWTC 3 cut(s) 88, 313, 742
PflFI GACNNNGTC 1 cut(s) 23
PkrI GCNGC 3 cut(s) 58, 380, 861
Ple19I CGATCG 1 cut(s) 1007
PleI GAGTC 3 cut(s) 109, 569, 962
PpsI GAGTC 3 cut(s) 109, 569, 962
PscI ACATGT 1 cut(s) 295
Psp1406I AACGTT 1 cut(s) 854
PspFI CCCAGC 1 cut(s) 46
PspN4I GGNNCC 3 cut(s) 15, 73, 505
PstNI CAGNNNCTG 1 cut(s) 745
PsuI RGATCY 1 cut(s) 176
PsyI GACNNNGTC 1 cut(s) 23
PvuI CGATCG 1 cut(s) 1007
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
RseI CAYNNNNRTG 1 cut(s) 501
SaqAI TTAA 4 cut(s) 248, 267, 768, 810
SatI GCNGC 3 cut(s) 57, 379, 860
Sau3AI GATC 6 cut(s) 176, 328, 355, 1000, 1004, 1044
SchI GAGTC 3 cut(s) 110, 569, 962
SfaNI GCATC 3 cut(s) 261, 772, 1002
SfuI TTCGAA 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
Sse9I AATT 5 cut(s) 210, 269, 303, 692, 973
SsiI CCGC 4 cut(s) 56, 59, 233, 500
SspMI CTAG 1 cut(s) 750
TaaI ACNGT 2 cut(s) 40, 246
TaiI ACGT 3 cut(s) 27, 829, 857
TaqI TCGA 4 cut(s) 182, 342, 1003, 1007
TasI AATT 5 cut(s) 210, 269, 303, 692, 973
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 3 cut(s) 88, 313, 742
Tru1I TTAA 4 cut(s) 248, 267, 768, 810
Tru9I TTAA 4 cut(s) 248, 267, 768, 810
TscAI CASTG 2 cut(s) 111, 264
TseFI GTSAC 4 cut(s) 298, 350, 437, 456
TseI GCWGC 2 cut(s) 378, 859
Tsp45I GTSAC 4 cut(s) 298, 350, 437, 456
TspDTI ATGAA 4 cut(s) 151, 576, 663, 914
TspGWI ACGGA 2 cut(s) 525, 856
TspRI CASTG 2 cut(s) 111, 264
Tth111I GACNNNGTC 1 cut(s) 23
Vha464I CTTAAG 1 cut(s) 809
XagI CCTNNNNNAGG 1 cut(s) 770
XapI RAATTY 2 cut(s) 269, 973
XbaI TCTAGA 1 cut(s) 749
XceI RCATGY 2 cut(s) 299, 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 440
XspI CTAG 1 cut(s) 750
ZraI GACGTC 1 cut(s) 25
Zsp2I ATGCAT 1 cut(s) 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.