pycom07g25360

septum site-determining protein minD homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0017186
Physical Location & Seq
Forward (+)
25695866 .. 25697025
1160 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
ATGCTCTCTCTTCAACTCCTCCCCGCCCTCCTCATCCCCAAGCCTTCCTCCTCTTCCTCCCTCACCGCCGCAAAACCACCAAAAACCTTTAAACCCAAAACCCTAAAACCCAAACCTTCTCCCACCTTCACAGTCCGCGCCATCCTCCAATACAACAGGAAGCCCCAATTGTCCGGCGATACCCCCCGCGTCGTCGTCATCACCTCTGGAAAAGGAGGCGTTGGCAAGACCACGACCACCGCCAATGTCGGCCTCTCCCTCGCCCGCCTCGGCTTCTCCGTGGTTGCCATCGACGCCGACGTCGGCCTCCGCAACCTGGACCTCCTCCTCGGCCTCGAGAACCGCGTCAACTACACCGTCGTTGAGGTCCTCAACGGCGACTGCCGCCTCGACCAGGCGCTTGTCAGGGACAAGCGCTGGTCCAACTTCGAATTGCTCTGCATCTCAAAGCCCCGGTCCAAATTGCCGATGGGGTTCGGCGGAAAGGCCCTGGTCTGGGTCGTCGACGCTTTGAAAGCCAGGCAAGAGGGCTGCCCGGACTTCATCCTCATTGACTGCCCCGCCGGAATCGACGCCGGCTTCATCACCGCCATTACGCCGGCCAATGAGGCCGTGCTGGTGACCACGCCGGATATCACAAGCTTGAGAGACGCTGACAGAGTCATTGGGCTGCTGGAGTGTGATGGAATTAGGGATATAAAGATGATGGTGAACCGGGTCCGGACAGATATGATCAAAGGGGAGGACATGATGTCGGTGCTCGATGTGCAGGAGATGTTGGGATTGGCATTGTTGGGGATGATTCCGGAGGACACGGAGGTCATCAGAAGCACCAACAGAGGGTACCCTCTGGTTTTGAATAGGCCGCCCACATTGGCAGGACTGGCCTTCGAGCAGGCAGCGTGGAGGCTTGTTGAGCAGGATAGTATGCAGGCTGTCATGGTGGAGGAAGAGCCCAAGAAGCGCGGCTTCTTCTCGTTTTTCGGAGGAGTAAGTCAGTTCAATTTTGTGATTGTGTCTCTGTTAGTGATAGCTAGAATTATAGATTTCTAG

Protein Analysis

351

Amino Acids

38.2

Weight (kDa)

8.76

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ParA PF10609 63 - 301 4.6e-17 NUBPL iron-transfer P-loop NTPase
AAA_31 PF13614 63 - 216 2.4e-14 AAA domain
MipZ PF09140 63 - 214 2.9e-07 ATPase MipZ
CbiA PF01656 65 - 283 1.6e-19 CobQ/CobB/MinD/ParA nucleotide binding domain
Fer4_NifH PF00142 69 - 308 1.5e-07 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017186)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24020
fragaria_vesca FvH4_7g30210
malus_domestica MD07G1278700.v1.1
prunus_persica Prupe.2G300600_v2.0.a1
pyrus_communis pycom07g25360
rosa_chinensis RchiOBHm_Chr1g0378251
rosa_laevigata RLG00000026456
rosa_multiflora Rmu_sc0006315.1_g000003 Rmu_sc0038642.1_g000001
rosa_roxburghii Rroxscaffold_4G00280610
rosa_rugosa Rorug01G0407500
rosa_samantha Rh1AG422300
rosa_wichuraiana Rw1G037530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 299, 818
AatII GACGTC 1 cut(s) 303
Acc65I GGTACC 1 cut(s) 843
AccB1I GGYRCC 1 cut(s) 843
AccI GTMKAC 1 cut(s) 504
AccII CGCG 4 cut(s) 138, 189, 345, 966
AccIII TCCGGA 2 cut(s) 720, 805
AcoI YGGCCR 1 cut(s) 600
AcyI GRCGYC 3 cut(s) 294, 300, 573
AfaI GTAC 1 cut(s) 845
AfeI AGCGCT 1 cut(s) 416
AfiI CCNNNNNNNGG 5 cut(s) 316, 394, 495, 496, 840
AgsI TTSAA 4 cut(s) 14, 514, 859, 1003
AhdI GACNNNNNGTC 1 cut(s) 751
AjnI CCWGG 4 cut(s) 315, 393, 489, 518
AluBI AGCT 2 cut(s) 642, 1034
AluI AGCT 2 cut(s) 642, 1034
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 2 cut(s) 642, 1023
Ama87I CYCGRG 1 cut(s) 335
Aor13HI TCCGGA 2 cut(s) 720, 805
Aor51HI AGCGCT 1 cut(s) 416
AoxI GGCC 8 cut(s) 250, 304, 331, 486, 600, 609, 863, 885
ApeKI GCWGC 3 cut(s) 531, 670, 899
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
Asp718I GGTACC 1 cut(s) 843
AspLEI GCGC 4 cut(s) 140, 400, 417, 966
AspS9I GGNCC 6 cut(s) 319, 367, 420, 456, 487, 718
AsuC2I CCSGG 3 cut(s) 454, 536, 716
AsuHPI GGTGA 5 cut(s) 55, 193, 577, 631, 721
AsuII TTCGAA 1 cut(s) 429
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 5 cut(s) 319, 367, 420, 456, 718
BanI GGYRCC 1 cut(s) 843
BanII GRGCYC 1 cut(s) 957
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 3 cut(s) 518, 657, 911
BccI CCATC 5 cut(s) 149, 296, 463, 677, 700
BceAI ACGGC 2 cut(s) 391, 596
BciT130I CCWGG 4 cut(s) 317, 395, 491, 520
BclI TGATCA 1 cut(s) 732
BcnI CCSGG 3 cut(s) 454, 536, 716
BcoDI GTCTC 2 cut(s) 642, 1023
BfaI CTAG 2 cut(s) 1035, 1051
BfoI RGCGCY 2 cut(s) 401, 418
BglI GCCNNNNNGGC 1 cut(s) 608
BisI GCNGC 7 cut(s) 69, 385, 532, 671, 866, 900, 967
BlsI GCNGC 7 cut(s) 70, 386, 533, 672, 867, 901, 968
Bme1390I CCNGG 7 cut(s) 317, 395, 454, 491, 520, 536, 716
Bme18I GGWCC 5 cut(s) 319, 367, 420, 456, 718
BmeRI GACNNNNNGTC 1 cut(s) 751
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 6 cut(s) 319, 367, 420, 456, 487, 718
BmiI GGNNCC 2 cut(s) 719, 845
BmrFI CCNGG 7 cut(s) 317, 395, 454, 491, 520, 536, 716
BmsI GCATC 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 695
Bpu14I TTCGAA 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 664
BpuMI CCSGG 3 cut(s) 454, 536, 716
BsaHI GRCGYC 3 cut(s) 294, 300, 573
BsaJI CCNNGG 5 cut(s) 268, 279, 328, 452, 489
BsaWI WCCGGW 2 cut(s) 720, 805
Bsc4I CCNNNNNNNGG 5 cut(s) 316, 394, 495, 496, 840
Bse118I RCCGGY 2 cut(s) 575, 598
Bse1I ACTGG 1 cut(s) 888
BseAI TCCGGA 2 cut(s) 720, 805
BseBI CCWGG 4 cut(s) 317, 395, 491, 520
BseDI CCNNGG 5 cut(s) 268, 279, 328, 452, 489
BseGI GGATG 4 cut(s) 33, 141, 543, 804
BseLI CCNNNNNNNGG 5 cut(s) 316, 394, 495, 496, 840
BseNI ACTGG 1 cut(s) 888
BseRI GAGGAG 6 cut(s) 8, 20, 40, 314, 317, 1002
BseXI GCAGC 3 cut(s) 518, 657, 911
BsgI GTGCAG 1 cut(s) 788
Bsh1236I CGCG 4 cut(s) 138, 189, 345, 966
BshFI GGCC 8 cut(s) 252, 306, 333, 488, 602, 611, 865, 887
BshNI GGYRCC 1 cut(s) 843
BsiHKAI GWGCWC 1 cut(s) 762
BsiHKCI CYCGRG 1 cut(s) 335
BslFI GGGAC 1 cut(s) 422
BslI CCNNNNNNNGG 5 cut(s) 316, 394, 495, 496, 840
BsmAI GTCTC 2 cut(s) 642, 1023
BsmBI CGTCTC 1 cut(s) 642
BsmFI GGGAC 1 cut(s) 422
BsnI GGCC 8 cut(s) 252, 306, 333, 488, 602, 611, 865, 887
BsoBI CYCGRG 1 cut(s) 335
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 762, 957
Bsp13I TCCGGA 2 cut(s) 720, 805
Bsp143I GATC 1 cut(s) 732
BspANI GGCC 8 cut(s) 252, 306, 333, 488, 602, 611, 865, 887
BspEI TCCGGA 2 cut(s) 720, 805
BspFNI CGCG 4 cut(s) 138, 189, 345, 966
BspLI GGNNCC 2 cut(s) 719, 845
BspQI GCTCTTC 1 cut(s) 945
BspT104I TTCGAA 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 843
BsrFI RCCGGY 2 cut(s) 575, 598
BsrI ACTGG 1 cut(s) 888
BssAI RCCGGY 2 cut(s) 575, 598
BssECI CCNNGG 5 cut(s) 268, 279, 328, 452, 489
BssMI GATC 1 cut(s) 732
BssNI GRCGYC 3 cut(s) 294, 300, 573
Bst2UI CCWGG 4 cut(s) 317, 395, 491, 520
Bst4CI ACNGT 2 cut(s) 133, 358
Bst6I CTCTTC 3 cut(s) 15, 58, 945
BstACI GRCGYC 3 cut(s) 294, 300, 573
BstBI TTCGAA 1 cut(s) 429
BstC8I GCNNGC 5 cut(s) 265, 577, 600, 897, 933
BstDSI CCRYGG 1 cut(s) 279
BstEII GGTNACC 1 cut(s) 619
BstENI CCTNNNNNAGG 1 cut(s) 392
BstF5I GGATG 4 cut(s) 33, 141, 543, 804
BstFNI CGCG 4 cut(s) 138, 189, 345, 966
BstH2I RGCGCY 2 cut(s) 401, 418
BstHHI GCGC 4 cut(s) 140, 400, 417, 966
BstKTI GATC 1 cut(s) 735
BstMAI GTCTC 2 cut(s) 642, 1023
BstMBI GATC 1 cut(s) 732
BstMWI GCNNNNNNNGC 8 cut(s) 293, 384, 515, 608, 766, 884, 916, 961
BstNI CCWGG 4 cut(s) 317, 395, 491, 520
BstPI GGTNACC 1 cut(s) 619
BstSCI CCNGG 7 cut(s) 315, 393, 452, 489, 518, 534, 714
BstUI CGCG 4 cut(s) 138, 189, 345, 966
BstV1I GCAGC 3 cut(s) 518, 657, 911
BsuRI GGCC 8 cut(s) 252, 306, 333, 488, 602, 611, 865, 887
BtgI CCRYGG 1 cut(s) 279
BtsCI GGATG 4 cut(s) 33, 141, 543, 804
Cac8I GCNNGC 5 cut(s) 265, 577, 600, 897, 933
CfoI GCGC 4 cut(s) 140, 400, 417, 966
Cfr10I RCCGGY 2 cut(s) 575, 598
Cfr13I GGNCC 6 cut(s) 319, 367, 420, 456, 487, 718
CseI GACGC 6 cut(s) 178, 302, 334, 515, 581, 659
Csp6I GTAC 1 cut(s) 844
CviAII CATG 2 cut(s) 748, 940
CviQI GTAC 1 cut(s) 844
DpnI GATC 1 cut(s) 734
DpnII GATC 1 cut(s) 732
DraI TTTAAA 1 cut(s) 91
DrdI GACNNNNNNGTC 2 cut(s) 299, 818
DriI GACNNNNNGTC 1 cut(s) 751
DseDI GACNNNNNNGTC 2 cut(s) 299, 818
EaeI YGGCCR 1 cut(s) 600
Eam1104I CTCTTC 3 cut(s) 15, 58, 945
Eam1105I GACNNNNNGTC 1 cut(s) 751
EarI CTCTTC 3 cut(s) 15, 58, 945
EciI GGCGGA 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 957
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 5 cut(s) 319, 367, 420, 456, 718
Eco47III AGCGCT 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 335
Eco91I GGTNACC 1 cut(s) 619
EcoNI CCTNNNNNAGG 1 cut(s) 392
EcoO109I RGGNCCY 2 cut(s) 367, 487
EcoO65I GGTNACC 1 cut(s) 619
EcoRII CCWGG 4 cut(s) 315, 393, 489, 518
EcoRV GATATC 1 cut(s) 634
EcoT38I GRGCYC 1 cut(s) 957
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 2 cut(s) 751, 943
FaiI YATR 6 cut(s) 698, 731, 749, 929, 941, 1043
FalI AAGNNNNNCTT 2 cut(s) 953, 985
FaqI GGGAC 1 cut(s) 422
FatI CATG 2 cut(s) 747, 939
FauI CCCGC 4 cut(s) 31, 194, 272, 568
FbaI TGATCA 1 cut(s) 732
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 7 cut(s) 69, 385, 532, 671, 866, 900, 967
FokI GGATG 4 cut(s) 20, 128, 530, 811
FriOI GRGCYC 1 cut(s) 957
Fsp4HI GCNGC 7 cut(s) 69, 385, 532, 671, 866, 900, 967
FspBI CTAG 2 cut(s) 1035, 1051
GlaI GCGC 4 cut(s) 139, 399, 416, 965
GluI GCNGC 7 cut(s) 69, 385, 532, 671, 866, 900, 967
GsuI CTGGAG 1 cut(s) 695
HaeII RGCGCY 2 cut(s) 401, 418
HaeIII GGCC 8 cut(s) 252, 306, 333, 488, 602, 611, 865, 887
HgaI GACGC 6 cut(s) 178, 302, 334, 515, 581, 659
HhaI GCGC 4 cut(s) 140, 400, 417, 966
Hin1I GRCGYC 3 cut(s) 294, 300, 573
Hin1II CATG 2 cut(s) 751, 943
Hin6I GCGC 4 cut(s) 138, 398, 415, 964
HinP1I GCGC 4 cut(s) 138, 398, 415, 964
HincII GTYRAC 2 cut(s) 349, 505
HindII GTYRAC 2 cut(s) 349, 505
HindIII AAGCTT 1 cut(s) 640
HinfI GANTC 3 cut(s) 567, 660, 802
HphI GGTGA 5 cut(s) 55, 193, 577, 631, 721
Hpy166II GTNNAC 3 cut(s) 349, 505, 712
Hpy188I TCNGA 2 cut(s) 827, 986
Hpy188III TCNNGA 4 cut(s) 207, 337, 721, 806
Hpy8I GTNNAC 3 cut(s) 349, 505, 712
Hpy99I CGWCG 9 cut(s) 194, 197, 296, 302, 305, 362, 506, 509, 575
HpyAV CCTTC 4 cut(s) 54, 126, 136, 898
HpyCH4III ACNGT 2 cut(s) 133, 358
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 3 cut(s) 441, 769, 931
HpyF10VI GCNNNNNNNGC 8 cut(s) 293, 384, 515, 608, 766, 884, 916, 961
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 3 cut(s) 294, 300, 573
Hsp92II CATG 2 cut(s) 751, 943
HspAI GCGC 4 cut(s) 138, 398, 415, 964
Kpn2I TCCGGA 2 cut(s) 720, 805
KpnI GGTACC 1 cut(s) 847
KroI GCCGGC 2 cut(s) 575, 598
KroNI GCCGGC 2 cut(s) 577, 600
Ksp22I TGATCA 1 cut(s) 732
Kzo9I GATC 1 cut(s) 732
LguI GCTCTTC 1 cut(s) 945
Lsp1109I GCAGC 3 cut(s) 518, 657, 911
LweI GCATC 1 cut(s) 450
MaeI CTAG 2 cut(s) 1035, 1051
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 1 cut(s) 619
MalI GATC 1 cut(s) 734
MboI GATC 1 cut(s) 732
MboII GAAGA 3 cut(s) 45, 962, 964
MfeI CAATTG 1 cut(s) 167
MhlI GDGCHC 2 cut(s) 762, 957
MluCI AATT 6 cut(s) 167, 431, 461, 687, 1003, 1038
MlyI GAGTC 1 cut(s) 669
MmeI TCCRAC 1 cut(s) 447
MroI TCCGGA 2 cut(s) 720, 805
MroNI GCCGGC 2 cut(s) 575, 598
MseI TTAA 1 cut(s) 90
MspR9I CCNGG 7 cut(s) 317, 395, 454, 491, 520, 536, 716
MunI CAATTG 1 cut(s) 167
MvaI CCWGG 4 cut(s) 317, 395, 491, 520
MvnI CGCG 4 cut(s) 138, 189, 345, 966
MwoI GCNNNNNNNGC 8 cut(s) 293, 384, 515, 608, 766, 884, 916, 961
NaeI GCCGGC 2 cut(s) 577, 600
NciI CCSGG 3 cut(s) 454, 536, 716
NdeII GATC 1 cut(s) 732
NgoMIV GCCGGC 2 cut(s) 575, 598
NlaIII CATG 2 cut(s) 751, 943
NlaIV GGNNCC 2 cut(s) 719, 845
NmeAIII GCCGAG 2 cut(s) 249, 309
NmuCI GTSAC 1 cut(s) 619
NspV TTCGAA 1 cut(s) 429
PaeR7I CTCGAG 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 945
PcsI WCGNNNNNNNCGW 3 cut(s) 276, 297, 342
PdiI GCCGGC 2 cut(s) 577, 600
PfeI GAWTC 2 cut(s) 567, 802
PflFI GACNNNGTC 1 cut(s) 659
PkrI GCNGC 7 cut(s) 70, 386, 533, 672, 867, 901, 968
PleI GAGTC 1 cut(s) 668
PpsI GAGTC 1 cut(s) 668
PpuMI RGGWCCY 1 cut(s) 367
Psp5II RGGWCCY 1 cut(s) 367
Psp6I CCWGG 4 cut(s) 315, 393, 489, 518
PspEI GGTNACC 1 cut(s) 619
PspGI CCWGG 4 cut(s) 315, 393, 489, 518
PspN4I GGNNCC 2 cut(s) 719, 845
PspPI GGNCC 6 cut(s) 319, 367, 420, 456, 487, 718
PspPPI RGGWCCY 1 cut(s) 367
PsyI GACNNNGTC 1 cut(s) 659
RsaI GTAC 1 cut(s) 845
RsaNI GTAC 1 cut(s) 844
SalI GTCGAC 1 cut(s) 503
SapI GCTCTTC 1 cut(s) 945
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 7 cut(s) 69, 385, 532, 671, 866, 900, 967
Sau3AI GATC 1 cut(s) 732
Sau96I GGNCC 6 cut(s) 319, 367, 420, 456, 487, 718
SchI GAGTC 1 cut(s) 669
ScrFI CCNGG 7 cut(s) 317, 395, 454, 491, 520, 536, 716
SduI GDGCHC 2 cut(s) 762, 957
SfaNI GCATC 1 cut(s) 450
SfiI GGCCNNNNNGGCC 1 cut(s) 608
Sfr274I CTCGAG 1 cut(s) 335
SfuI TTCGAA 1 cut(s) 429
SgrDI CGTCGACG 1 cut(s) 503
SinI GGWCC 5 cut(s) 319, 367, 420, 456, 718
SlaI CTCGAG 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 335, 643
SmoI CTYRAG 2 cut(s) 335, 643
Sse9I AATT 6 cut(s) 167, 431, 461, 687, 1003, 1038
SspMI CTAG 2 cut(s) 1035, 1051
StyD4I CCNGG 7 cut(s) 315, 393, 452, 489, 518, 534, 714
TaaI ACNGT 2 cut(s) 133, 358
TaiI ACGT 1 cut(s) 303
TaqI TCGA 8 cut(s) 291, 336, 390, 429, 504, 570, 762, 891
TasI AATT 6 cut(s) 167, 431, 461, 687, 1003, 1038
TauI GCSGC 4 cut(s) 71, 387, 868, 969
TfiI GAWTC 2 cut(s) 567, 802
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 3 cut(s) 531, 670, 899
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 2 cut(s) 532, 571
TspGWI ACGGA 2 cut(s) 268, 830
Tth111I GACNNNGTC 1 cut(s) 659
VpaK11BI GGWCC 5 cut(s) 319, 367, 420, 456, 718
XagI CCTNNNNNAGG 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XhoI CTCGAG 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 2 cut(s) 1035, 1051
ZraI GACGTC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.