pycom07g26260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006079
Physical Location & Seq
Reverse (-)
26298622 .. 26302206
3585 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2427 bp
ATGGCGCGGTTACTAAGTGAAAAATACTTCAATGGCTTGACGGAGTGGCCTCAAAACTTTACCCGTTTGGACTGGTTGGTTAGAATTGCTGAGGTTGAAGTTGAGTTAATGCAGCAAAAGAGAGAAACTTTGATGAGAGAGGGGGCTCTCAAGAAGCCAAGGCTAGTCGAGCCGCCCAAGAAACAAAGGTCGCCCCCTATCGATTCTACTAGAACAGATCATTCTCTGAAAAGAAAATGGTCTAGCAATGGGATAAATGATGATCGTGCGCCGAAGAAACAGAGGTCGTGCGATGGAGCGAAGCTTACGGCTTCTGCTATGGAGTCTTCACTGAGAGAGACTAATGCTATTGGTAGGGGTGACGTGGGATGGAAGAAACGAAAGGTGATGCCGAAGAACAATGTCAAGAAAGCCAAGGGATCGACCAAGAACTCTCCGAAGAAGAAAGAACCGATTGGTAGTCCTTCCACGCCTCCGAAATATGACGACTTGCCTGACGAATTCAAGAGAGAAATTGCAACGTTAAAGGGGACTAGGCTGGGCTTGGTGATACAGAAGCGAATTACCATGACTGATATATCTCCCGGCGAAAACAGACTGTCAATGCCTGAAGCCCAAATTGTATCGATGGATTTCTTGGAAGGGAAAGAGAAGGAGTTACTTGAGGGACAGCAAACAATGAAGGTTCAGTTAATAGACCCTGGTCTTGTAAAAGGAGATATAAACTTGAGACTCTGGCTTATGAAAAGCAGCAAACTGTATGTGTTGAGAACTCAATGGGGTGCGGTTGCTCAGAGGAACAAAATCAAACAAGGCCAGCTTGTTCAGGTTTGGTCATTCCGGGTTAATGGAGCGCTTTACTTGGCTCTGGTTTTGGTTAAGGAGAGTGGCGGTAAGGGGGATTGTAAGGAGAGTCATGGCAGCGATAGCGGGGGTAGGAGTATAGCTGTGGAGGGTGAAACAAGTGGTGGTAGTGGAGGAGGAACTGAGATAATGGAAGAGAAAAAGGATGATAACAACGGGAATGCTAGTGCGGCAAGTACTGTGATCAATGGGTCCGACCATGCAAGTCCCAACCCTGGAGGAGACCATGCATGTCCCAACCCTGGTGGAGACCATGCAAGTCCCAACCCTAGTGGAAGCAGCAAAGAAGATAAACCAAAAACAAAGCCAATGGCGTTGTCTCAGTCGTCGCCTATCCTTCCTCAAAACCCAGCGAGCTCCATTGGGCGTCGACAGAGAGATGGCTGCAGCAAAGGTACGAAGAGATACAGGGCTGGATTGAGCTCGGGGTACGGCCATGTGGCGCAGAGCTGGATTAGGGTTTTTCACTTCCTGCGGGGTACGGCCATGTGGCCCAGAGCTGGATTGAGCTCGGGACTGAACCTCCGGAAGCTCTCAACCGCAGTACGACGCCGTATCGAGGACGAAGGCGACTGGTCCTACGCCTCGGAGTGGCGGGGGGAAGAATCCGAAGGCCACACCGTCCTCCGCTCCACATCTGACAAAGGCAATGGCGTTGTCTCAGTCGTCGCCTATCCTTCCTCAAAACCCAGCGAACTCCATTGGGCATCGACAGAGAGATGGCTGCAGCAAAGATACGAAGAGATACAGGAGTGCGGTCAGGACAACGAGAGATTCAGAGTGCTCGGATATCAATGGCGCGCTCTCCGCTTTAACGACGACACCCGCCAGAGCACCGTTAAGGTTTTGGCTGCCTATCGGCGATCGGAACCGGAATCGATCGCCCTTATGCAGCAGCCTCATTGCTTGGCTCTTCCTTATCTTAAGAGTATGATAGCAGCTGGGTTGGCCACTATAGCATCTTGTAATTACAATCTCATGAACGCGGTGACTGGGACCGAAAATATTCGTATATTGTGCATTGGTCAGGGTGGCGGAAGCTTACCGTTGTTTTTGGCTAGTAAAATTCAAGGTGCTATTGTTGATGTTGTTGAAATCGACCCTCTTGTTATCTCAGCCTCAGTTCGAGCCATGGGATTTCCGGCTTTCTCAATTATGACTCCATCAGGCAAGCGTGCCGTGTCACCCAATACCATGGATACCGTTATGTGGAAAGGCATCCACGAGAGGCTGTTCCTCCATGAATCCGATGCTGAGGATTTCGTTCTCAATACCACCATTCAGTATGATATGGTCTTCGTTGATGCTTATGATGGGGAAGACATATTCCCACGCAAGTTATGGGATCCACATTCCCTATTTCTTAAAGCCCTCAGCATTGTGGTGAACCTTCATTCAGATTCTGATATCTTGAACCCTGACGGTTCCGCACCATCCGTTCTGCAGCAAACTTTGCCAATGGGGAAGTACGTCTCGAGGGTTTGCCTGCAGGCATACAAGGACGTGGTAGTGGGGAGGAAAGATTCTGGTTTGGGGTTTACAAGGTTTTGTGGTTACGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

809

Amino Acids

89.19

Weight (kDa)

9.32

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 180 - 263 5.6e-14 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1494
AccI GTMKAC 1 cut(s) 1234
AccII CGCG 3 cut(s) 7, 1665, 1850
AccIII TCCGGA 1 cut(s) 1389
AclI AACGTT 1 cut(s) 521
AclWI GGATC 3 cut(s) 427, 2204, 2217
AcoI YGGCCR 3 cut(s) 1297, 1347, 1812
AcsI RAATTY 2 cut(s) 500, 1929
AcuI CTGAAG 1 cut(s) 630
AcyI GRCGYC 2 cut(s) 1231, 1414
AfaI GTAC 6 cut(s) 1042, 1261, 1295, 1345, 1410, 2333
AfeI AGCGCT 1 cut(s) 855
AfiI CCNNNNNNNGG 6 cut(s) 1079, 1106, 1364, 1423, 1455, 2073
AflII CTTAAG 1 cut(s) 1787
AgsI TTSAA 6 cut(s) 31, 98, 505, 1934, 1958, 2278
AjiI CACGTC 2 cut(s) 364, 2368
AjnI CCWGG 3 cut(s) 700, 1078, 1105
Alw21I GWGCWC 5 cut(s) 1223, 1289, 1376, 1650, 1700
Alw26I GTCTC 7 cut(s) 332, 724, 1080, 1107, 1188, 1528, 2341
AlwI GGATC 3 cut(s) 427, 2204, 2217
AlwNI CAGNNNCTG 1 cut(s) 2267
Ama87I CYCGRG 3 cut(s) 1288, 1375, 2338
Aor13HI TCCGGA 1 cut(s) 1389
Aor51HI AGCGCT 1 cut(s) 855
AoxI GGCC 7 cut(s) 47, 814, 1297, 1347, 1355, 1477, 1812
ApoI RAATTY 2 cut(s) 500, 1929
Asp700I GAANNNNTTC 1 cut(s) 1869
AspLEI GCGC 6 cut(s) 7, 271, 856, 1309, 1665, 1667
AspS9I GGNCC 4 cut(s) 1056, 1356, 1440, 1860
AsuC2I CCSGG 2 cut(s) 585, 842
AsuHPI GGTGA 7 cut(s) 371, 397, 559, 968, 1864, 2040, 2260
AvaI CYCGRG 3 cut(s) 1288, 1375, 2338
AvaII GGWCC 3 cut(s) 1056, 1440, 1860
BaeI ACNNNNGTAYC 2 cut(s) 1601, 1634
BalI TGGCCA 1 cut(s) 1814
BamHI GGATCC 1 cut(s) 2209
BanII GRGCYC 4 cut(s) 148, 1223, 1289, 1376
BauI CACGAG 1 cut(s) 2087
BbsI GAAGAC 3 cut(s) 318, 2152, 2190
Bbv12I GWGCWC 5 cut(s) 1223, 1289, 1376, 1650, 1700
BbvCI CCTCAGC 3 cut(s) 90, 2118, 2237
BccI CCATC 8 cut(s) 287, 363, 622, 1238, 1578, 2035, 2171, 2305
BceAI ACGGC 5 cut(s) 324, 1312, 1362, 1401, 2027
BciT130I CCWGG 3 cut(s) 702, 1080, 1107
BciVI GTATCC 1 cut(s) 2056
BclI TGATCA 1 cut(s) 1047
BcnI CCSGG 2 cut(s) 585, 842
BcoDI GTCTC 7 cut(s) 332, 724, 1080, 1107, 1188, 1528, 2341
BfaI CTAG 7 cut(s) 164, 210, 243, 534, 1029, 1134, 1923
BfmI CTRYAG 5 cut(s) 1249, 1589, 1818, 2306, 2351
BfoI RGCGCY 1 cut(s) 857
BfrI CTTAAG 1 cut(s) 1787
BfuI GTATCC 1 cut(s) 2056
BmcAI AGTACT 1 cut(s) 1042
Bme1390I CCNGG 5 cut(s) 585, 702, 842, 1080, 1107
Bme18I GGWCC 3 cut(s) 1056, 1440, 1860
BmeT110I CYCGRG 3 cut(s) 1288, 1375, 2338
BmgBI CACGTC 2 cut(s) 364, 2368
BmgT120I GGNCC 4 cut(s) 1056, 1356, 1440, 1860
BmiI GGNNCC 5 cut(s) 1057, 1734, 1861, 2211, 2290
BmrFI CCNGG 5 cut(s) 585, 702, 842, 1080, 1107
BmrI ACTGGG 1 cut(s) 1866
BmsI GCATC 6 cut(s) 378, 1580, 1832, 2091, 2104, 2158
BmuI ACTGGG 1 cut(s) 1866
BoxI GACNNNNGTC 1 cut(s) 702
BpiI GAAGAC 3 cut(s) 318, 2152, 2190
BpmI CTGGAG 1 cut(s) 1101
Bpu10I CCTNAGC 3 cut(s) 90, 2118, 2237
BpuEI CTTGAG 3 cut(s) 134, 683, 748
BpuMI CCSGG 2 cut(s) 585, 842
Bsa29I ATCGAT 3 cut(s) 201, 626, 1742
BsaHI GRCGYC 2 cut(s) 1231, 1414
BsaI GGTCTC 2 cut(s) 1080, 1107
BsaJI CCNNGG 8 cut(s) 158, 414, 700, 1078, 1105, 1449, 1995, 2058
BsaWI WCCGGW 2 cut(s) 1389, 1735
BsaXI ACNNNNNCTCC 2 cut(s) 1371, 1401
Bsc4I CCNNNNNNNGG 6 cut(s) 1079, 1106, 1364, 1423, 1455, 2073
Bse1I ACTGG 3 cut(s) 77, 1442, 1861
Bse3DI GCAATG 3 cut(s) 253, 1519, 1765
BseAI TCCGGA 1 cut(s) 1389
BseBI CCWGG 3 cut(s) 702, 1080, 1107
BseCI ATCGAT 3 cut(s) 201, 626, 1742
BseDI CCNNGG 8 cut(s) 158, 414, 700, 1078, 1105, 1449, 1995, 2058
BseGI GGATG 4 cut(s) 374, 1015, 2082, 2297
BseLI CCNNNNNNNGG 6 cut(s) 1079, 1106, 1364, 1423, 1455, 2073
BseMI GCAATG 3 cut(s) 253, 1519, 1765
BseNI ACTGG 3 cut(s) 77, 1442, 1861
BsePI GCGCGC 1 cut(s) 1663
BseRI GAGGAG 2 cut(s) 993, 1098
BseYI CCCAGC 4 cut(s) 538, 1213, 1553, 1805
Bsh1236I CGCG 3 cut(s) 7, 1665, 1850
Bsh1285I CGRYCG 2 cut(s) 1730, 1746
BshFI GGCC 7 cut(s) 49, 816, 1299, 1349, 1357, 1479, 1814
BshVI ATCGAT 3 cut(s) 201, 626, 1742
BsiEI CGRYCG 2 cut(s) 1730, 1746
BsiHKAI GWGCWC 5 cut(s) 1223, 1289, 1376, 1650, 1700
BsiHKCI CYCGRG 3 cut(s) 1288, 1375, 2338
BsiSI CCGG 5 cut(s) 585, 841, 1390, 1736, 2006
BslFI GGGAC 7 cut(s) 544, 681, 1056, 1083, 1110, 1392, 1873
BslI CCNNNNNNNGG 6 cut(s) 1079, 1106, 1364, 1423, 1455, 2073
BsmAI GTCTC 7 cut(s) 332, 724, 1080, 1107, 1188, 1528, 2341
BsmBI CGTCTC 1 cut(s) 2341
BsmFI GGGAC 7 cut(s) 544, 681, 1056, 1083, 1110, 1392, 1873
BsmI GAATGC 1 cut(s) 1030
BsnI GGCC 7 cut(s) 49, 816, 1299, 1349, 1357, 1479, 1814
Bso31I GGTCTC 2 cut(s) 1080, 1107
BsoBI CYCGRG 3 cut(s) 1288, 1375, 2338
Bsp1286I GDGCHC 6 cut(s) 148, 1223, 1289, 1376, 1650, 1700
Bsp13I TCCGGA 1 cut(s) 1389
Bsp143I GATC 7 cut(s) 217, 262, 419, 1047, 1727, 1743, 2209
Bsp19I CCATGG 2 cut(s) 1995, 2058
BspANI GGCC 7 cut(s) 49, 816, 1299, 1349, 1357, 1479, 1814
BspDI ATCGAT 3 cut(s) 201, 626, 1742
BspEI TCCGGA 1 cut(s) 1389
BspFNI CGCG 3 cut(s) 7, 1665, 1850
BspHI TCATGA 1 cut(s) 1842
BspLI GGNNCC 5 cut(s) 1057, 1734, 1861, 2211, 2290
BspMAI CTGCAG 4 cut(s) 1253, 1593, 2310, 2355
BspPI GGATC 3 cut(s) 427, 2204, 2217
BspQI GCTCTTC 1 cut(s) 1782
BspTI CTTAAG 1 cut(s) 1787
BspTNI GGTCTC 2 cut(s) 1080, 1107
BsrBI CCGCTC 1 cut(s) 1494
BsrDI GCAATG 3 cut(s) 253, 1519, 1765
BsrI ACTGG 3 cut(s) 77, 1442, 1861
BssECI CCNNGG 8 cut(s) 158, 414, 700, 1078, 1105, 1449, 1995, 2058
BssHII GCGCGC 1 cut(s) 1663
BssMI GATC 7 cut(s) 217, 262, 419, 1047, 1727, 1743, 2209
BssNI GRCGYC 2 cut(s) 1231, 1414
BssSI CACGAG 1 cut(s) 2087
BssT1I CCWWGG 4 cut(s) 158, 414, 1995, 2058
Bst2BI CACGAG 1 cut(s) 2087
Bst2UI CCWGG 3 cut(s) 702, 1080, 1107
Bst4CI ACNGT 8 cut(s) 600, 759, 1045, 1486, 1702, 1911, 2068, 2288
Bst6I CTCTTC 4 cut(s) 993, 1259, 1599, 1782
BstACI GRCGYC 2 cut(s) 1231, 1414
BstAFI CTTAAG 1 cut(s) 1787
BstAPI GCANNNNNTGC 1 cut(s) 2317
BstC8I GCNNGC 7 cut(s) 818, 1219, 1665, 2036, 2040, 2351, 2355
BstDSI CCRYGG 2 cut(s) 1995, 2058
BstF5I GGATG 4 cut(s) 374, 1015, 2082, 2297
BstFNI CGCG 3 cut(s) 7, 1665, 1850
BstH2I RGCGCY 1 cut(s) 857
BstHHI GCGC 6 cut(s) 7, 271, 856, 1309, 1665, 1667
BstKTI GATC 7 cut(s) 220, 265, 422, 1050, 1730, 1746, 2212
BstMAI GTCTC 7 cut(s) 332, 724, 1080, 1107, 1188, 1528, 2341
BstMBI GATC 7 cut(s) 217, 262, 419, 1047, 1727, 1743, 2209
BstMCI CGRYCG 2 cut(s) 1730, 1746
BstMWI GCNNNNNNNGC 7 cut(s) 169, 927, 1034, 1671, 1811, 1820, 2317
BstNI CCWGG 3 cut(s) 702, 1080, 1107
BstNSI RCATGY 1 cut(s) 1098
BstPAI GACNNNNGTC 1 cut(s) 702
BstSCI CCNGG 5 cut(s) 583, 700, 840, 1078, 1105
BstSFI CTRYAG 5 cut(s) 1249, 1589, 1818, 2306, 2351
BstUI CGCG 3 cut(s) 7, 1665, 1850
BstV2I GAAGAC 3 cut(s) 318, 2152, 2190
BstX2I RGATCY 1 cut(s) 2209
BstXI CCANNNNNNTGG 1 cut(s) 2059
BstYI RGATCY 1 cut(s) 2209
Bsu15I ATCGAT 3 cut(s) 201, 626, 1742
BsuI GTATCC 1 cut(s) 2056
BsuRI GGCC 7 cut(s) 49, 816, 1299, 1349, 1357, 1479, 1814
BsuTUI ATCGAT 3 cut(s) 201, 626, 1742
BtgI CCRYGG 2 cut(s) 1995, 2058
BtgZI GCGATG 1 cut(s) 306
BtrI CACGTC 2 cut(s) 364, 2368
BtsCI GGATG 4 cut(s) 374, 1015, 2082, 2297
BtsIMutI CAGTG 1 cut(s) 329
Cac8I GCNNGC 7 cut(s) 818, 1219, 1665, 2036, 2040, 2351, 2355
CaiI CAGNNNCTG 1 cut(s) 2267
CciI TCATGA 1 cut(s) 1842
CfoI GCGC 6 cut(s) 7, 271, 856, 1309, 1665, 1667
Cfr13I GGNCC 4 cut(s) 1056, 1356, 1440, 1860
ClaI ATCGAT 3 cut(s) 201, 626, 1742
CseI GACGC 2 cut(s) 1220, 1422
Csp6I GTAC 6 cut(s) 1041, 1260, 1294, 1344, 1409, 2332
CspCI CAANNNNNGTGG 4 cut(s) 1090, 1117, 1125, 1152
CviQI GTAC 6 cut(s) 1041, 1260, 1294, 1344, 1409, 2332
DpnI GATC 7 cut(s) 219, 264, 421, 1049, 1729, 1745, 2211
DpnII GATC 7 cut(s) 217, 262, 419, 1047, 1727, 1743, 2209
EaeI YGGCCR 3 cut(s) 1297, 1347, 1812
Eam1104I CTCTTC 4 cut(s) 993, 1259, 1599, 1782
EarI CTCTTC 4 cut(s) 993, 1259, 1599, 1782
EciI GGCGGA 1 cut(s) 1914
Ecl136II GAGCTC 3 cut(s) 1221, 1287, 1374
Eco130I CCWWGG 4 cut(s) 158, 414, 1995, 2058
Eco24I GRGCYC 4 cut(s) 148, 1223, 1289, 1376
Eco31I GGTCTC 2 cut(s) 1080, 1107
Eco32I GATATC 2 cut(s) 1655, 2272
Eco47I GGWCC 3 cut(s) 1056, 1440, 1860
Eco47III AGCGCT 1 cut(s) 855
Eco53kI GAGCTC 3 cut(s) 1221, 1287, 1374
Eco57I CTGAAG 1 cut(s) 630
Eco88I CYCGRG 3 cut(s) 1288, 1375, 2338
EcoICRI GAGCTC 3 cut(s) 1221, 1287, 1374
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 3 cut(s) 700, 1078, 1105
EcoRV GATATC 2 cut(s) 1655, 2272
EcoT14I CCWWGG 4 cut(s) 158, 414, 1995, 2058
EcoT22I ATGCAT 1 cut(s) 1096
EcoT38I GRGCYC 4 cut(s) 148, 1223, 1289, 1376
ErhI CCWWGG 4 cut(s) 158, 414, 1995, 2058
Esp3I CGTCTC 1 cut(s) 2341
FalI AAGNNNNNCTT 2 cut(s) 804, 836
FaqI GGGAC 7 cut(s) 544, 681, 1056, 1083, 1110, 1392, 1873
FauI CCCGC 4 cut(s) 923, 1332, 1452, 1697
FbaI TGATCA 1 cut(s) 1047
FblI GTMKAC 1 cut(s) 1234
FokI GGATG 4 cut(s) 381, 1022, 2069, 2284
FriOI GRGCYC 4 cut(s) 148, 1223, 1289, 1376
FspBI CTAG 7 cut(s) 164, 210, 243, 534, 1029, 1134, 1923
GlaI GCGC 6 cut(s) 6, 270, 855, 1308, 1664, 1666
GsaI CCCAGC 4 cut(s) 542, 1217, 1557, 1809
GsuI CTGGAG 1 cut(s) 1101
HaeII RGCGCY 1 cut(s) 857
HaeIII GGCC 7 cut(s) 49, 816, 1299, 1349, 1357, 1479, 1814
HapII CCGG 5 cut(s) 585, 841, 1390, 1736, 2006
HgaI GACGC 2 cut(s) 1220, 1422
HhaI GCGC 6 cut(s) 7, 271, 856, 1309, 1665, 1667
Hin1I GRCGYC 2 cut(s) 1231, 1414
Hin6I GCGC 6 cut(s) 5, 269, 854, 1307, 1663, 1665
HinP1I GCGC 6 cut(s) 5, 269, 854, 1307, 1663, 1665
HincII GTYRAC 1 cut(s) 1235
HindII GTYRAC 1 cut(s) 1235
HindIII AAGCTT 2 cut(s) 302, 1903
HpaII CCGG 5 cut(s) 585, 841, 1390, 1736, 2006
HphI GGTGA 7 cut(s) 371, 397, 559, 968, 1864, 2040, 2260
Hpy166II GTNNAC 3 cut(s) 1235, 2251, 2403
Hpy188III TCNNGA 9 cut(s) 151, 406, 505, 1377, 1390, 1625, 1843, 2275, 2338
Hpy8I GTNNAC 3 cut(s) 1235, 2251, 2403
Hpy99I CGWCG 5 cut(s) 1195, 1236, 1416, 1535, 1685
HpyAV CCTTC 9 cut(s) 474, 635, 646, 676, 1211, 1424, 1469, 1551, 2264
HpyCH4III ACNGT 8 cut(s) 600, 759, 1045, 1486, 1702, 1911, 2068, 2288
HpyCH4IV ACGT 4 cut(s) 363, 521, 2334, 2367
HpyF10VI GCNNNNNNNGC 7 cut(s) 169, 927, 1034, 1671, 1811, 1820, 2317
HpySE526I ACGT 4 cut(s) 363, 521, 2334, 2367
Hsp92I GRCGYC 2 cut(s) 1231, 1414
HspAI GCGC 6 cut(s) 5, 269, 854, 1307, 1663, 1665
Kpn2I TCCGGA 1 cut(s) 1389
Ksp22I TGATCA 1 cut(s) 1047
Kzo9I GATC 7 cut(s) 217, 262, 419, 1047, 1727, 1743, 2209
LguI GCTCTTC 1 cut(s) 1782
LmnI GCTCC 4 cut(s) 296, 851, 1226, 1499
LweI GCATC 6 cut(s) 378, 1580, 1832, 2091, 2104, 2158
MaeI CTAG 7 cut(s) 164, 210, 243, 534, 1029, 1134, 1923
MaeII ACGT 4 cut(s) 363, 521, 2334, 2367
MaeIII GTNAC 6 cut(s) 9, 359, 657, 1852, 2046, 2417
MalI GATC 7 cut(s) 219, 264, 421, 1049, 1729, 1745, 2211
MbiI CCGCTC 1 cut(s) 1494
MboI GATC 7 cut(s) 217, 262, 419, 1047, 1727, 1743, 2209
MflI RGATCY 1 cut(s) 2209
MhlI GDGCHC 6 cut(s) 148, 1223, 1289, 1376, 1650, 1700
MlsI TGGCCA 1 cut(s) 1814
MluCI AATT 8 cut(s) 84, 500, 513, 561, 618, 1831, 1929, 2016
MluNI TGGCCA 1 cut(s) 1814
MlyI GAGTC 4 cut(s) 332, 726, 922, 2017
MmeI TCCRAC 1 cut(s) 1083
Mox20I TGGCCA 1 cut(s) 1814
Mph1103I ATGCAT 1 cut(s) 1096
MroI TCCGGA 1 cut(s) 1389
MroXI GAANNNNTTC 1 cut(s) 1869
MscI TGGCCA 1 cut(s) 1814
MseI TTAA 9 cut(s) 107, 524, 692, 846, 879, 1677, 1704, 1788, 2229
MslI CAYNNNNRTG 1 cut(s) 2246
Msp20I TGGCCA 1 cut(s) 1814
MspA1I CMGCKG 1 cut(s) 1805
MspCI CTTAAG 1 cut(s) 1787
MspI CCGG 5 cut(s) 585, 841, 1390, 1736, 2006
MspR9I CCNGG 5 cut(s) 585, 702, 842, 1080, 1107
Mva1269I GAATGC 1 cut(s) 1030
MvaI CCWGG 3 cut(s) 702, 1080, 1107
MvnI CGCG 3 cut(s) 7, 1665, 1850
MwoI GCNNNNNNNGC 7 cut(s) 169, 927, 1034, 1671, 1811, 1820, 2317
NciI CCSGG 2 cut(s) 585, 842
NcoI CCATGG 2 cut(s) 1995, 2058
NdeII GATC 7 cut(s) 217, 262, 419, 1047, 1727, 1743, 2209
NlaIV GGNNCC 5 cut(s) 1057, 1734, 1861, 2211, 2290
NmuCI GTSAC 3 cut(s) 359, 1852, 2046
NsiI ATGCAT 1 cut(s) 1096
NspI RCATGY 1 cut(s) 1098
PaeR7I CTCGAG 1 cut(s) 2338
PagI TCATGA 1 cut(s) 1842
PauI GCGCGC 1 cut(s) 1663
PciSI GCTCTTC 1 cut(s) 1782
PctI GAATGC 1 cut(s) 1030
PdmI GAANNNNTTC 1 cut(s) 1869
PfeI GAWTC 7 cut(s) 203, 1469, 1638, 1739, 2108, 2264, 2387
Ple19I CGATCG 2 cut(s) 1730, 1746
PleI GAGTC 4 cut(s) 331, 726, 921, 2017
PpsI GAGTC 4 cut(s) 331, 726, 921, 2017
PshAI GACNNNNGTC 1 cut(s) 702
Psp124BI GAGCTC 3 cut(s) 1223, 1289, 1376
Psp1406I AACGTT 1 cut(s) 521
Psp6I CCWGG 3 cut(s) 700, 1078, 1105
PspFI CCCAGC 4 cut(s) 538, 1213, 1553, 1805
PspGI CCWGG 3 cut(s) 700, 1078, 1105
PspN4I GGNNCC 5 cut(s) 1057, 1734, 1861, 2211, 2290
PspPI GGNCC 4 cut(s) 1056, 1356, 1440, 1860
PstI CTGCAG 4 cut(s) 1253, 1593, 2310, 2355
PstNI CAGNNNCTG 1 cut(s) 2267
PsuI RGATCY 1 cut(s) 2209
PteI GCGCGC 1 cut(s) 1663
PvuI CGATCG 2 cut(s) 1730, 1746
PvuII CAGCTG 1 cut(s) 1805
RsaI GTAC 6 cut(s) 1042, 1261, 1295, 1345, 1410, 2333
RsaNI GTAC 6 cut(s) 1041, 1260, 1294, 1344, 1409, 2332
RseI CAYNNNNRTG 1 cut(s) 2246
SacI GAGCTC 3 cut(s) 1223, 1289, 1376
SalI GTCGAC 1 cut(s) 1233
SapI GCTCTTC 1 cut(s) 1782
SaqAI TTAA 9 cut(s) 107, 524, 692, 846, 879, 1677, 1704, 1788, 2229
Sau3AI GATC 7 cut(s) 217, 262, 419, 1047, 1727, 1743, 2209
Sau96I GGNCC 4 cut(s) 1056, 1356, 1440, 1860
SbfI CCTGCAGG 1 cut(s) 2355
ScaI AGTACT 1 cut(s) 1042
SchI GAGTC 4 cut(s) 332, 726, 922, 2017
ScrFI CCNGG 5 cut(s) 585, 702, 842, 1080, 1107
SdaI CCTGCAGG 1 cut(s) 2355
SduI GDGCHC 6 cut(s) 148, 1223, 1289, 1376, 1650, 1700
SfaNI GCATC 6 cut(s) 378, 1580, 1832, 2091, 2104, 2158
SfcI CTRYAG 5 cut(s) 1249, 1589, 1818, 2306, 2351
Sfr274I CTCGAG 1 cut(s) 2338
SinI GGWCC 3 cut(s) 1056, 1440, 1860
SlaI CTCGAG 1 cut(s) 2338
SmiMI CAYNNNNRTG 1 cut(s) 2246
SmlI CTYRAG 5 cut(s) 149, 662, 727, 1787, 2338
SmoI CTYRAG 5 cut(s) 149, 662, 727, 1787, 2338
Sse8387I CCTGCAGG 1 cut(s) 2355
Sse9I AATT 8 cut(s) 84, 500, 513, 561, 618, 1831, 1929, 2016
SspI AATATT 1 cut(s) 1870
SspMI CTAG 7 cut(s) 164, 210, 243, 534, 1029, 1134, 1923
SstI GAGCTC 3 cut(s) 1223, 1289, 1376
StyD4I CCNGG 5 cut(s) 583, 700, 840, 1078, 1105
StyI CCWWGG 4 cut(s) 158, 414, 1995, 2058
TaaI ACNGT 8 cut(s) 600, 759, 1045, 1486, 1702, 1911, 2068, 2288
TaiI ACGT 4 cut(s) 366, 524, 2337, 2370
TaqII GACCGA 1 cut(s) 1877
TasI AATT 8 cut(s) 84, 500, 513, 561, 618, 1831, 1929, 2016
TatI WGTACW 1 cut(s) 1040
TauI GCSGC 2 cut(s) 175, 1037
TfiI GAWTC 7 cut(s) 203, 1469, 1638, 1739, 2108, 2264, 2387
Tru1I TTAA 9 cut(s) 107, 524, 692, 846, 879, 1677, 1704, 1788, 2229
Tru9I TTAA 9 cut(s) 107, 524, 692, 846, 879, 1677, 1704, 1788, 2229
TscAI CASTG 1 cut(s) 336
TseFI GTSAC 3 cut(s) 359, 1852, 2046
Tsp45I GTSAC 3 cut(s) 359, 1852, 2046
TspDTI ATGAA 5 cut(s) 695, 758, 1859, 2121, 2246
TspGWI ACGGA 2 cut(s) 56, 2290
TspRI CASTG 1 cut(s) 336
Vha464I CTTAAG 1 cut(s) 1787
VpaK11BI GGWCC 3 cut(s) 1056, 1440, 1860
XapI RAATTY 2 cut(s) 500, 1929
XceI RCATGY 1 cut(s) 1098
XcmI CCANNNNNNNNNTGG 1 cut(s) 2202
XhoI CTCGAG 1 cut(s) 2338
XmiI GTMKAC 1 cut(s) 1234
XmnI GAANNNNTTC 1 cut(s) 1869
XspI CTAG 7 cut(s) 164, 210, 243, 534, 1029, 1134, 1923
ZrmI AGTACT 1 cut(s) 1042
Zsp2I ATGCAT 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.