pycom07g27550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0018089
Physical Location & Seq
Reverse (-)
27233104 .. 27233631
528 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g27550.1

Sequence Viewer

Length: 528 bp
ATGGGAGGCTGTGCGAGCAAGCCAAAGGAGTTTGACGCCCCTCGTCCAGAGACCCTCCCTAGCGAGACCACCAGCCCCAAGAAGGCTGATGGCCCTCAGGAGGGTGAGGCCACTACTACAACCACCACCGCCACTACCACCGAGGCTGAGAAACAAGAGGAGACCACCACTGAAGCACCCTTGGTAGACCTCTCAAAGCCAAAGGAGAAGGGGGCTGAGGTTGCGGCCGCACCTGCTGTTGTCCCTGCCATTGCGGCCGAGGCTGAGGTGGTAGCAACTGAGAAGCCAAAAGAGGAAGTGGTTGATCAAGCCGAAGTTGTTGCCAAGCAACTGGCGGAGATTAATCTCGTTGAGGCTGTGAAGGAGAATAAGGGGAAAGAGCTGGTGGAGGAGCCTGCACCGAAGGCGAAAGAAGAGGAGCCAGCGGCGGAGATCAAGCCAAAGGCCACGGAGAAGGATTTAGCAAAGGCAGCAGCTCCGGCCGCGGCAGAGGACAAGGCTAAGGATGCACCTCTTGTTACTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

18.18

Weight (kDa)

4.6

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018089)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g33240
prunus_persica Prupe.2G321400_v2.0.a1
pyrus_communis pycom07g27550
rosa_laevigata RLG00000026121
rosa_multiflora Rmu_sc0007173.1_g000026
rosa_roxburghii Rroxscaffold_4G00277490
rosa_samantha Rh1AG465300 Rh1BG422700 Rh1CG435900 Rh1DG455400
rosa_wichuraiana Rw1G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 241
Acc36I ACCTGC 1 cut(s) 241
AccI GTMKAC 1 cut(s) 186
AccII CGCG 1 cut(s) 485
AciI CCGC 9 cut(s) 129, 224, 228, 254, 335, 425, 428, 483, 485
AcoI YGGCCR 3 cut(s) 225, 255, 480
AcuI CTGAAG 1 cut(s) 192
AcyI GRCGYC 1 cut(s) 36
AfiI CCNNNNNNNGG 3 cut(s) 82, 100, 101
AluBI AGCT 2 cut(s) 382, 476
AluI AGCT 2 cut(s) 382, 476
Alw26I GTCTC 3 cut(s) 44, 59, 155
AoxI GGCC 6 cut(s) 91, 108, 225, 255, 444, 480
ApeKI GCWGC 2 cut(s) 470, 473
AseI ATTAAT 1 cut(s) 342
AspS9I GGNCC 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 116
AxyI CCTNAGG 1 cut(s) 96
BbvCI CCTCAGC 2 cut(s) 216, 264
BbvI GCAGC 2 cut(s) 482, 485
BccI CCATC 1 cut(s) 83
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BclI TGATCA 1 cut(s) 304
BcoDI GTCTC 3 cut(s) 44, 59, 155
BfaI CTAG 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 241
BglI GCCNNNNNGGC 1 cut(s) 254
BisI GCNGC 8 cut(s) 225, 228, 255, 426, 471, 474, 483, 486
BlsI GCNGC 8 cut(s) 226, 229, 256, 427, 472, 475, 484, 487
BmgT120I GGNCC 1 cut(s) 92
BmiI GGNNCC 2 cut(s) 393, 420
BmsI GCATC 1 cut(s) 496
Bpu10I CCTNAGC 3 cut(s) 216, 264, 501
BsaHI GRCGYC 1 cut(s) 36
BsaI GGTCTC 3 cut(s) 44, 59, 155
BsaJI CCNNGG 5 cut(s) 141, 180, 258, 447, 483
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 100, 101
Bse1I ACTGG 1 cut(s) 336
Bse21I CCTNAGG 1 cut(s) 96
Bse3DI GCAATG 1 cut(s) 249
BseDI CCNNGG 5 cut(s) 141, 180, 258, 447, 483
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 3 cut(s) 82, 100, 101
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 5 cut(s) 110, 138, 207, 255, 270
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 3 cut(s) 173, 404, 431
BseX3I CGGCCG 3 cut(s) 225, 255, 480
BseXI GCAGC 2 cut(s) 482, 485
BsgI GTGCAG 1 cut(s) 381
Bsh1236I CGCG 1 cut(s) 485
Bsh1285I CGRYCG 3 cut(s) 228, 258, 483
BshFI GGCC 6 cut(s) 93, 110, 227, 257, 446, 482
BsiEI CGRYCG 3 cut(s) 228, 258, 483
BsiSI CCGG 1 cut(s) 479
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 3 cut(s) 82, 100, 101
BsmAI GTCTC 3 cut(s) 44, 59, 155
BsmFI GGGAC 1 cut(s) 227
BsnI GGCC 6 cut(s) 93, 110, 227, 257, 446, 482
Bso31I GGTCTC 3 cut(s) 44, 59, 155
Bsp143I GATC 2 cut(s) 304, 432
BspACI CCGC 9 cut(s) 129, 224, 228, 254, 335, 425, 428, 483, 485
BspANI GGCC 6 cut(s) 93, 110, 227, 257, 446, 482
BspCNI CTCAG 5 cut(s) 109, 139, 208, 256, 271
BspFNI CGCG 1 cut(s) 485
BspLI GGNNCC 2 cut(s) 393, 420
BspMI ACCTGC 1 cut(s) 241
BspTNI GGTCTC 3 cut(s) 44, 59, 155
BsrDI GCAATG 1 cut(s) 249
BsrI ACTGG 1 cut(s) 336
BssECI CCNNGG 5 cut(s) 141, 180, 258, 447, 483
BssMI GATC 2 cut(s) 304, 432
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 523
Bst6I CTCTTC 1 cut(s) 408
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 4 cut(s) 16, 20, 396, 423
BstDEI CTNAG 6 cut(s) 96, 147, 216, 264, 279, 501
BstDSI CCRYGG 2 cut(s) 447, 483
BstF5I GGATG 1 cut(s) 511
BstFNI CGCG 1 cut(s) 485
BstKTI GATC 2 cut(s) 307, 435
BstMAI GTCTC 3 cut(s) 44, 59, 155
BstMBI GATC 2 cut(s) 304, 432
BstMCI CGRYCG 3 cut(s) 228, 258, 483
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 2 cut(s) 482, 485
BstXI CCANNNNNNTGG 1 cut(s) 331
BstZI CGGCCG 3 cut(s) 225, 255, 480
Bsu36I CCTNAGG 1 cut(s) 96
BsuRI GGCC 6 cut(s) 93, 110, 227, 257, 446, 482
BtgI CCRYGG 2 cut(s) 447, 483
BtsCI GGATG 1 cut(s) 511
BtsIMutI CAGTG 1 cut(s) 168
BveI ACCTGC 1 cut(s) 241
Cac8I GCNNGC 4 cut(s) 16, 20, 396, 423
CciNI GCGGCCGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 92
Cfr42I CCGCGG 1 cut(s) 486
CseI GACGC 1 cut(s) 44
DdeI CTNAG 6 cut(s) 96, 147, 216, 264, 279, 501
DpnI GATC 2 cut(s) 306, 434
DpnII GATC 2 cut(s) 304, 432
EaeI YGGCCR 3 cut(s) 225, 255, 480
EagI CGGCCG 3 cut(s) 225, 255, 480
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EciI GGCGGA 2 cut(s) 350, 443
EclXI CGGCCG 3 cut(s) 225, 255, 480
Eco130I CCWWGG 1 cut(s) 180
Eco31I GGTCTC 3 cut(s) 44, 59, 155
Eco52I CGGCCG 3 cut(s) 225, 255, 480
Eco57I CTGAAG 1 cut(s) 192
Eco81I CCTNAGG 1 cut(s) 96
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaqI GGGAC 1 cut(s) 227
FbaI TGATCA 1 cut(s) 304
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 8 cut(s) 225, 228, 255, 426, 471, 474, 483, 486
FokI GGATG 1 cut(s) 518
Fsp4HI GCNGC 8 cut(s) 225, 228, 255, 426, 471, 474, 483, 486
FspBI CTAG 1 cut(s) 60
GluI GCNGC 8 cut(s) 225, 228, 255, 426, 471, 474, 483, 486
HaeIII GGCC 6 cut(s) 93, 110, 227, 257, 446, 482
HapII CCGG 1 cut(s) 479
HgaI GACGC 1 cut(s) 44
Hin1I GRCGYC 1 cut(s) 36
HpaII CCGG 1 cut(s) 479
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 1 cut(s) 187
Hpy188III TCNNGA 2 cut(s) 47, 98
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 5 cut(s) 76, 202, 355, 397, 448
HpyCH4III ACNGT 1 cut(s) 523
HpyCH4V TGCA 2 cut(s) 398, 509
HpyF3I CTNAG 6 cut(s) 96, 147, 216, 264, 279, 501
Hsp92I GRCGYC 1 cut(s) 36
Ksp22I TGATCA 1 cut(s) 304
KspI CCGCGG 1 cut(s) 486
Kzo9I GATC 2 cut(s) 304, 432
LmnI GCTCC 3 cut(s) 391, 418, 481
Lsp1109I GCAGC 2 cut(s) 482, 485
LweI GCATC 1 cut(s) 496
MaeI CTAG 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 517
MalI GATC 2 cut(s) 306, 434
MboI GATC 2 cut(s) 304, 432
MboII GAAGA 1 cut(s) 425
MseI TTAA 1 cut(s) 342
MspA1I CMGCKG 2 cut(s) 425, 485
MspI CCGG 1 cut(s) 479
MvnI CGCG 1 cut(s) 485
NdeII GATC 2 cut(s) 304, 432
NlaIV GGNNCC 2 cut(s) 393, 420
NmeAIII GCCGAG 1 cut(s) 283
NotI GCGGCCGC 1 cut(s) 225
PaqCI CACCTGC 1 cut(s) 241
PkrI GCNGC 8 cut(s) 226, 229, 256, 427, 472, 475, 484, 487
PshBI ATTAAT 1 cut(s) 342
PspN4I GGNNCC 2 cut(s) 393, 420
PspPI GGNCC 1 cut(s) 92
SacII CCGCGG 1 cut(s) 486
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 8 cut(s) 225, 228, 255, 426, 471, 474, 483, 486
Sau3AI GATC 2 cut(s) 304, 432
Sau96I GGNCC 1 cut(s) 92
SetI ASST 7 cut(s) 192, 222, 235, 270, 384, 478, 514
SfaNI GCATC 1 cut(s) 496
Sfr303I CCGCGG 1 cut(s) 486
SgrBI CCGCGG 1 cut(s) 486
SsiI CCGC 9 cut(s) 129, 224, 228, 254, 335, 425, 428, 483, 485
SspMI CTAG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 523
TauI GCSGC 6 cut(s) 227, 230, 257, 428, 485, 488
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 175
TseI GCWGC 2 cut(s) 470, 473
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 1 cut(s) 175
VspI ATTAAT 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.