pycom08g02160

Belongs to the bacterial ribosomal protein bL31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012462
Physical Location & Seq
Reverse (-)
1608992 .. 1609512
521 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g02160.1

Sequence Viewer

Length: 420 bp
ATGGCGCTCAATCTAAACACCTCATTCCTCCATGGAACACCCTGCCCCACGCTCCTCCCTCCCAAGAAGGTCTGCGGTGGCGGTGGGTCGTCGGCGGGGAATTGCCGCTCTGTGGTGACGTGCAGGAAGAAGGAGATACACCCGCAGTTCCACGAGGATGCGAAGGTGTACTGCAAGGGGGAGCTGGTGATGACGACGGGTGGGACCCAGAAGGAGTACGTGGTGGACGTGTGGTCGGGCAACCACCCCTTCTACCTCGGAAACCGGTCGGGCATGCTCGTCGACGACGACCAGGTCGAGAAGTTCAGGAAGAAGTACGGCGAGCTCACCCAGATCATGGAGATTCCTGTCCTCAAGGGAGAGATTATTCTGCCCTCCCGCCGCAAAGCCGCCGCCGGTAAAGGCGGCAAGAAGAAGTAG

Protein Analysis

140

Amino Acids

15.21

Weight (kDa)

9.44

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L31 PF01197 43 - 107 4.3e-17 Ribosomal protein L31
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012462)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75350
fragaria_vesca FvH4_2g39490 FvH4_2g39490
malus_domestica MD08G1027600.v1.1 MD15G1023800.v1.1
prunus_persica Prupe.1G376100_v2.0.a1
pyrus_communis pycom08g02160 pycom15g02040
rosa_chinensis RchiOBHm_Chr6g0301461
rosa_laevigata RLG00000011251
rosa_multiflora Rmu_co8470853.1_g000001 Rmu_sc0014417.1_g000013
rosa_roxburghii Rroxscaffold_7G00166780
rosa_rugosa Rorug06G0305000
rosa_samantha Rh6AG418600 Rh6BG424800 Rh6CG432100 Rh6DG418100
rosa_wichuraiana Rw6G036220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 337
AccBSI CCGCTC 1 cut(s) 108
AccI GTMKAC 1 cut(s) 282
AfaI GTAC 3 cut(s) 170, 218, 317
AfiI CCNNNNNNNGG 2 cut(s) 112, 337
AflIII ACRYGT 1 cut(s) 228
AgeI ACCGGT 1 cut(s) 264
AhdI GACNNNNNGTC 1 cut(s) 232
AjiI CACGTC 2 cut(s) 120, 229
AjnI CCWGG 1 cut(s) 291
AluBI AGCT 2 cut(s) 184, 325
AluI AGCT 2 cut(s) 184, 325
Alw21I GWGCWC 1 cut(s) 327
AsiGI ACCGGT 1 cut(s) 264
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 204
AsuHPI GGTGA 3 cut(s) 127, 199, 319
AvaII GGWCC 1 cut(s) 204
BanII GRGCYC 1 cut(s) 327
BauI CACGAG 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 327
BceAI ACGGC 1 cut(s) 334
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciT130I CCWGG 1 cut(s) 293
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 5 cut(s) 106, 382, 390, 393, 406
BlsI GCNGC 5 cut(s) 107, 383, 391, 394, 407
Bme1390I CCNGG 1 cut(s) 293
Bme18I GGWCC 1 cut(s) 204
BmeRI GACNNNNNGTC 1 cut(s) 232
BmgBI CACGTC 2 cut(s) 120, 229
BmgT120I GGNCC 1 cut(s) 204
BmiI GGNNCC 2 cut(s) 205, 206
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 148
BpuEI CTTGAG 1 cut(s) 338
BsaAI YACGTR 1 cut(s) 220
BsaJI CCNNGG 2 cut(s) 31, 256
BsaWI WCCGGW 1 cut(s) 264
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 337
Bse118I RCCGGY 2 cut(s) 264, 395
BseBI CCWGG 1 cut(s) 293
BseDI CCNNGG 2 cut(s) 31, 256
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 2 cut(s) 112, 337
BseRI GAGGAG 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 142
Bsh1285I CGRYCG 1 cut(s) 269
BshTI ACCGGT 1 cut(s) 264
BsiEI CGRYCG 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 327
BsiSI CCGG 2 cut(s) 265, 396
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 2 cut(s) 112, 337
BsmFI GGGAC 1 cut(s) 217
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 1 cut(s) 333
Bsp19I CCATGG 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 205, 206
BsrBI CCGCTC 1 cut(s) 108
BsrFI RCCGGY 2 cut(s) 264, 395
BssAI RCCGGY 2 cut(s) 264, 395
BssECI CCNNGG 2 cut(s) 31, 256
BssMI GATC 1 cut(s) 333
BssSI CACGAG 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 2 cut(s) 275, 323
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 1 cut(s) 163
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 336
BstMBI GATC 1 cut(s) 333
BstMCI CGRYCG 1 cut(s) 269
BstNI CCWGG 1 cut(s) 293
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 291
BtgI CCRYGG 1 cut(s) 31
BtrI CACGTC 2 cut(s) 120, 229
BtsCI GGATG 1 cut(s) 163
Cac8I GCNNGC 2 cut(s) 275, 323
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 2 cut(s) 264, 395
Cfr13I GGNCC 1 cut(s) 204
CsiI ACCWGGT 1 cut(s) 291
Csp6I GTAC 3 cut(s) 169, 217, 316
CspAI ACCGGT 1 cut(s) 264
CviAII CATG 3 cut(s) 32, 274, 337
CviJI RGCY 3 cut(s) 184, 325, 389
CviKI_1 RGCY 3 cut(s) 184, 325, 389
CviQI GTAC 3 cut(s) 169, 217, 316
DpnI GATC 1 cut(s) 335
DpnII GATC 1 cut(s) 333
DrdI GACNNNNNNGTC 1 cut(s) 293
DriI GACNNNNNGTC 1 cut(s) 232
DseDI GACNNNNNNGTC 1 cut(s) 293
Eam1105I GACNNNNNGTC 1 cut(s) 232
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 204
Eco53kI GAGCTC 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 325
EcoO109I RGGNCCY 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 3 cut(s) 35, 277, 340
FaiI YATR 3 cut(s) 33, 275, 338
FaqI GGGAC 1 cut(s) 217
FatI CATG 3 cut(s) 31, 273, 336
FauI CCCGC 3 cut(s) 88, 150, 386
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 5 cut(s) 106, 382, 390, 393, 406
FokI GGATG 1 cut(s) 170
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 5 cut(s) 106, 382, 390, 393, 406
GlaI GCGC 1 cut(s) 6
GluI GCNGC 5 cut(s) 106, 382, 390, 393, 406
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 2 cut(s) 265, 396
HhaI GCGC 1 cut(s) 7
Hin1II CATG 3 cut(s) 35, 277, 340
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 1 cut(s) 343
HpaII CCGG 2 cut(s) 265, 396
HphI GGTGA 3 cut(s) 127, 199, 319
Hpy166II GTNNAC 3 cut(s) 169, 226, 283
Hpy188I TCNGA 1 cut(s) 260
Hpy188III TCNNGA 2 cut(s) 298, 307
Hpy8I GTNNAC 3 cut(s) 169, 226, 283
Hpy99I CGWCG 5 cut(s) 94, 199, 284, 287, 290
HpyAV CCTTC 5 cut(s) 61, 124, 157, 205, 259
HpyCH4IV ACGT 3 cut(s) 119, 219, 228
HpyCH4V TGCA 2 cut(s) 123, 174
HpySE526I ACGT 3 cut(s) 119, 219, 228
Hsp92II CATG 3 cut(s) 35, 277, 340
HspAI GCGC 1 cut(s) 5
KflI GGGWCCC 1 cut(s) 204
Kzo9I GATC 1 cut(s) 333
LmnI GCTCC 2 cut(s) 57, 181
LweI GCATC 1 cut(s) 148
MabI ACCWGGT 1 cut(s) 291
MaeII ACGT 3 cut(s) 119, 219, 228
MaeIII GTNAC 1 cut(s) 115
MalI GATC 1 cut(s) 335
MbiI CCGCTC 1 cut(s) 108
MboI GATC 1 cut(s) 333
MboII GAAGA 2 cut(s) 139, 322
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 1 cut(s) 100
MnlI CCTC 8 cut(s) 31, 38, 65, 69, 148, 266, 362, 385
MslI CAYNNNNRTG 1 cut(s) 156
MspI CCGG 2 cut(s) 265, 396
MspR9I CCNGG 1 cut(s) 293
MvaI CCWGG 1 cut(s) 293
NcoI CCATGG 1 cut(s) 31
NdeII GATC 1 cut(s) 333
NlaIII CATG 3 cut(s) 35, 277, 340
NlaIV GGNNCC 2 cut(s) 205, 206
NmuCI GTSAC 1 cut(s) 115
NspI RCATGY 1 cut(s) 277
PaeI GCATGC 1 cut(s) 277
PcsI WCGNNNNNNNCGW 3 cut(s) 225, 285, 294
PfeI GAWTC 1 cut(s) 343
PflFI GACNNNGTC 1 cut(s) 293
PflMI CCANNNNNTGG 1 cut(s) 337
PinAI ACCGGT 1 cut(s) 264
PkrI GCNGC 5 cut(s) 107, 383, 391, 394, 407
Ppu21I YACGTR 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 2 cut(s) 205, 206
PspPI GGNCC 1 cut(s) 204
PspPPI RGGWCCY 1 cut(s) 204
PsyI GACNNNGTC 1 cut(s) 293
RsaI GTAC 3 cut(s) 170, 218, 317
RsaNI GTAC 3 cut(s) 169, 217, 316
RseI CAYNNNNRTG 1 cut(s) 156
SacI GAGCTC 1 cut(s) 327
SalI GTCGAC 1 cut(s) 281
SatI GCNGC 5 cut(s) 106, 382, 390, 393, 406
Sau3AI GATC 1 cut(s) 333
Sau96I GGNCC 1 cut(s) 204
ScrFI CCNGG 1 cut(s) 293
SduI GDGCHC 1 cut(s) 327
SexAI ACCWGGT 1 cut(s) 291
SfaNI GCATC 1 cut(s) 148
SgrDI CGTCGACG 1 cut(s) 281
SinI GGWCC 1 cut(s) 204
SmiMI CAYNNNNRTG 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SphI GCATGC 1 cut(s) 277
Sse9I AATT 1 cut(s) 100
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 1 cut(s) 291
StyI CCWWGG 1 cut(s) 31
TaiI ACGT 3 cut(s) 122, 222, 231
TaqI TCGA 2 cut(s) 282, 297
TasI AATT 1 cut(s) 100
TatI WGTACW 1 cut(s) 168
TauI GCSGC 5 cut(s) 108, 384, 392, 395, 408
TfiI GAWTC 1 cut(s) 343
TseFI GTSAC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 115
Tth111I GACNNNGTC 1 cut(s) 293
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 204
XceI RCATGY 1 cut(s) 277
XmiI GTMKAC 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.