pycom08g05320

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0016033
Physical Location & Seq
Reverse (-)
4097722 .. 4101412
3691 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1101 bp
ATGTTCAGTAATTTAATTCTTATTTCTTCAATCCCCTGGCGTTTAGAGAGAGAGAGAGAGAGAGAGAGAAAGAGAGGGATGTGGGAGTCGATCTTCCTAACGCTGGTGGCGACGGCCGGAAATAACATCGGCAAAATTCTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTGATCAGGGCTTATGCTTTGAACAAAGCATGGCTGATAGGTTTTCTGATGGATATATTTGGAGCTTTGCTAATGTTGAGAGCATTGTCCCTAGCACCTGTGTCTGTCATACAACCAGTTTCCGGGTGTGGACTTGCGATTCTCTCAATCTTTTCCCATTTTTATCTGAAGGAAATGATGAATGCTGTTGACTGGATGGGGATTACATTAGCAGGCATTGGCACTATAGGCGTTGGTGCCGGAGGTGAGGAGCAAAAGGCTTCCGCAATATCTGTGTTTCATCTACCATGGTTGGCAATTGTAGTCGCCTTCTTGTTTGCCCTCCTTAATGGGTGGCTACGCATCTACAGACGTCAACGCAAAAAACAGGAGTTGATGGAATATGAAGTTGTTGAAGAGATTATATGTGGCTTGGAATCTGGCATTTTGTTCGGGATGGCATCTGTAATATCGAAGATGGGATTTGTATTCTTGGAGCAGGGTTTCCACAGCATACTGGTTCCGATATGTGTCATAATCAGCATATGTTGTAGTGGTACAGGGTTTTATTATCAGACTTGGGGCTTAAAGCATGGGAGGGCAATAGTAGTGTCTACATGTGCTGCTGTGGCATCAATTGTGACTGGTGTTCTTGCTGGAATGCTTGCTTTGGGTGAAAAATTGCCTTCAGCACCAACAGCTCGTCTTTCACTGATGCTTGGATGGTTACTCATAATTGTCGGTGTGATTTTGCTTGTGAGCTCAACACGGCTGATGCGATGCCTTCCTCGACCATTACGACAACTTTTACAGAGTGGCGTTGATCAGAGGAAATCCGGGACTATCCGTGTTAGGGATGGAAGCCCGAGCACTGTTGTTCAGGCAGCAACTCTGCATCATTTGATACCAACTGCTTCGAAAGAGAAGGCTTGA

Protein Analysis

367

Amino Acids

40.08

Weight (kDa)

9.86

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 66 - 309 2.9e-06 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11960
fragaria_vesca FvH4_2g31600
malus_domestica MD08G1064900.v1.1 MD15G1056700.v1.1
prunus_persica Prupe.1G409300_v2.0.a1
pyrus_communis pycom08g05320 pycom15g05310
rosa_chinensis RchiOBHm_Chr6g0311221
rosa_laevigata RLG00000010426
rosa_roxburghii Rroxscaffold_7G00157890
rosa_rugosa Rorug06G0386600.1
rosa_samantha Rh6AG500700 Rh6BG511300 Rh6DG503600
rosa_wichuraiana Rw6G043600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 544
AccB1I GGYRCC 2 cut(s) 152, 425
AccI GTMKAC 1 cut(s) 782
AciI CCGC 1 cut(s) 453
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 1 cut(s) 135
AcuI CTGAAG 3 cut(s) 125, 377, 840
AcyI GRCGYC 1 cut(s) 541
AfaI GTAC 1 cut(s) 727
AfiI CCNNNNNNNGG 3 cut(s) 103, 311, 1021
AflIII ACRYGT 1 cut(s) 785
AgsI TTSAA 4 cut(s) 30, 181, 211, 584
AjnI CCWGG 1 cut(s) 35
AluBI AGCT 4 cut(s) 184, 254, 869, 930
AluI AGCT 4 cut(s) 184, 254, 869, 930
Alw21I GWGCWC 2 cut(s) 932, 1040
Ama87I CYCGRG 1 cut(s) 1033
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 2 cut(s) 791, 1052
ApoI RAATTY 1 cut(s) 135
AsuC2I CCSGG 2 cut(s) 313, 1006
AsuHPI GGTGA 3 cut(s) 202, 446, 854
AsuII TTCGAA 1 cut(s) 1085
AvaI CYCGRG 1 cut(s) 1033
BaeGI GKGCMC 1 cut(s) 155
BanI GGYRCC 2 cut(s) 152, 425
BanII GRGCYC 1 cut(s) 932
Bbv12I GWGCWC 2 cut(s) 932, 1040
BbvI GCAGC 2 cut(s) 778, 1064
BccI CCATC 7 cut(s) 232, 379, 559, 619, 640, 885, 1019
BceAI ACGGC 2 cut(s) 129, 953
BcgI CGANNNNNNTGC 2 cut(s) 601, 635
BciT130I CCWGG 1 cut(s) 37
BclI TGATCA 2 cut(s) 192, 991
BcnI CCSGG 2 cut(s) 313, 1006
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 2 cut(s) 414, 535
BisI GCNGC 2 cut(s) 792, 1053
BlsI GCNGC 2 cut(s) 793, 1054
Bme1390I CCNGG 3 cut(s) 37, 313, 1006
BmeT110I CYCGRG 1 cut(s) 1033
BmiI GGNNCC 3 cut(s) 154, 427, 690
BmrFI CCNGG 3 cut(s) 37, 313, 1006
BmsI GCATC 7 cut(s) 540, 638, 809, 873, 933, 938, 1072
Bpu14I TTCGAA 1 cut(s) 1085
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 2 cut(s) 313, 1006
BsaHI GRCGYC 1 cut(s) 541
BsaJI CCNNGG 2 cut(s) 35, 476
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 311, 1021
Bse1I ACTGG 4 cut(s) 305, 386, 690, 817
BseBI CCWGG 1 cut(s) 37
BseDI CCNNGG 2 cut(s) 35, 476
BseGI GGATG 5 cut(s) 84, 390, 630, 896, 1030
BseLI CCNNNNNNNGG 3 cut(s) 103, 311, 1021
BseNI ACTGG 4 cut(s) 305, 386, 690, 817
BseRI GAGGAG 2 cut(s) 159, 452
BseSI GKGCMC 1 cut(s) 155
BseX3I CGGCCG 1 cut(s) 114
BseXI GCAGC 2 cut(s) 778, 1064
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 1 cut(s) 116
BshNI GGYRCC 2 cut(s) 152, 425
BsiEI CGRYCG 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 932, 1040
BsiHKCI CYCGRG 1 cut(s) 1033
BsiSI CCGG 4 cut(s) 117, 312, 429, 1005
BslFI GGGAC 2 cut(s) 262, 1021
BslI CCNNNNNNNGG 3 cut(s) 103, 311, 1021
BsmFI GGGAC 2 cut(s) 262, 1021
BsmI GAATGC 2 cut(s) 376, 834
BsnI GGCC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 1033
Bsp119I TTCGAA 1 cut(s) 1085
Bsp1286I GDGCHC 3 cut(s) 155, 932, 1040
Bsp143I GATC 3 cut(s) 90, 192, 991
Bsp19I CCATGG 1 cut(s) 476
BspACI CCGC 1 cut(s) 453
BspANI GGCC 1 cut(s) 116
BspLI GGNNCC 3 cut(s) 154, 427, 690
BspT104I TTCGAA 1 cut(s) 1085
BspT107I GGYRCC 2 cut(s) 152, 425
BsrI ACTGG 4 cut(s) 305, 386, 690, 817
BssECI CCNNGG 2 cut(s) 35, 476
BssMI GATC 3 cut(s) 90, 192, 991
BssNI GRCGYC 1 cut(s) 541
BssT1I CCWWGG 1 cut(s) 476
Bst2UI CCWGG 1 cut(s) 37
Bst4CI ACNGT 2 cut(s) 157, 1042
Bst6I CTCTTC 1 cut(s) 579
BstACI GRCGYC 1 cut(s) 541
BstBI TTCGAA 1 cut(s) 1085
BstC8I GCNNGC 2 cut(s) 403, 834
BstDSI CCRYGG 1 cut(s) 476
BstF5I GGATG 5 cut(s) 84, 390, 630, 896, 1030
BstKTI GATC 3 cut(s) 93, 195, 994
BstMBI GATC 3 cut(s) 90, 192, 991
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 3 cut(s) 417, 797, 866
BstNI CCWGG 1 cut(s) 37
BstNSI RCATGY 1 cut(s) 789
BstSCI CCNGG 3 cut(s) 35, 311, 1004
BstSFI CTRYAG 2 cut(s) 414, 535
BstSLI GKGCMC 1 cut(s) 155
BstV1I GCAGC 2 cut(s) 778, 1064
BstZI CGGCCG 1 cut(s) 114
BsuRI GGCC 1 cut(s) 116
BtgI CCRYGG 1 cut(s) 476
BtgZI GCGATG 1 cut(s) 961
BtsCI GGATG 5 cut(s) 84, 390, 630, 896, 1030
BtsIMutI CAGTG 2 cut(s) 878, 1038
Cac8I GCNNGC 2 cut(s) 403, 834
Csp6I GTAC 1 cut(s) 726
CviAII CATG 4 cut(s) 219, 477, 761, 786
CviQI GTAC 1 cut(s) 726
DpnI GATC 3 cut(s) 92, 194, 993
DpnII GATC 3 cut(s) 90, 192, 991
EaeI YGGCCR 1 cut(s) 114
EagI CGGCCG 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
Ecl136II GAGCTC 1 cut(s) 930
EclXI CGGCCG 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 932
Eco52I CGGCCG 1 cut(s) 114
Eco53kI GAGCTC 1 cut(s) 930
Eco57I CTGAAG 3 cut(s) 125, 377, 840
Eco88I CYCGRG 1 cut(s) 1033
EcoICRI GAGCTC 1 cut(s) 930
EcoRII CCWGG 1 cut(s) 35
EcoT14I CCWWGG 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 932
ErhI CCWWGG 1 cut(s) 476
FaeI CATG 4 cut(s) 222, 480, 764, 789
FaqI GGGAC 2 cut(s) 262, 1021
FatI CATG 4 cut(s) 218, 476, 760, 785
FauNDI CATATG 1 cut(s) 713
FbaI TGATCA 2 cut(s) 192, 991
FblI GTMKAC 1 cut(s) 782
Fnu4HI GCNGC 2 cut(s) 792, 1053
FokI GGATG 5 cut(s) 91, 397, 637, 903, 1037
FriOI GRGCYC 1 cut(s) 932
Fsp4HI GCNGC 2 cut(s) 792, 1053
FspBI CTAG 1 cut(s) 281
GluI GCNGC 2 cut(s) 792, 1053
HaeIII GGCC 1 cut(s) 116
HapII CCGG 4 cut(s) 117, 312, 429, 1005
Hin1I GRCGYC 1 cut(s) 541
Hin1II CATG 4 cut(s) 222, 480, 764, 789
HincII GTYRAC 2 cut(s) 379, 545
HindII GTYRAC 2 cut(s) 379, 545
HinfI GANTC 3 cut(s) 86, 328, 605
HpaII CCGG 4 cut(s) 117, 312, 429, 1005
HphI GGTGA 3 cut(s) 202, 446, 854
Hpy166II GTNNAC 4 cut(s) 320, 379, 545, 783
Hpy188I TCNGA 6 cut(s) 144, 237, 357, 693, 744, 996
Hpy188III TCNNGA 1 cut(s) 622
Hpy8I GTNNAC 4 cut(s) 320, 379, 545, 783
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 6 cut(s) 142, 352, 508, 864, 962, 1087
HpyCH4III ACNGT 2 cut(s) 157, 1042
HpyCH4IV ACGT 1 cut(s) 541
HpyCH4V TGCA 1 cut(s) 1063
HpyF10VI GCNNNNNNNGC 3 cut(s) 417, 797, 866
HpySE526I ACGT 1 cut(s) 541
Hsp92I GRCGYC 1 cut(s) 541
Hsp92II CATG 4 cut(s) 222, 480, 764, 789
Ksp22I TGATCA 2 cut(s) 192, 991
Kzo9I GATC 3 cut(s) 90, 192, 991
LmnI GCTCC 3 cut(s) 251, 439, 664
Lsp1109I GCAGC 2 cut(s) 778, 1064
LweI GCATC 7 cut(s) 540, 638, 809, 873, 933, 938, 1072
MaeI CTAG 1 cut(s) 281
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 2 cut(s) 808, 894
MalI GATC 3 cut(s) 92, 194, 993
MboI GATC 3 cut(s) 90, 192, 991
MboII GAAGA 7 cut(s) 18, 85, 131, 155, 157, 596, 655
MfeI CAATTG 2 cut(s) 486, 804
MhlI GDGCHC 3 cut(s) 155, 932, 1040
MluCI AATT 7 cut(s) 10, 15, 135, 486, 804, 848, 903
MlyI GAGTC 1 cut(s) 95
MnlI CCTC 9 cut(s) 68, 177, 180, 425, 430, 521, 759, 966, 990
MseI TTAA 3 cut(s) 14, 516, 755
MspI CCGG 4 cut(s) 117, 312, 429, 1005
MspR9I CCNGG 3 cut(s) 37, 313, 1006
MunI CAATTG 2 cut(s) 486, 804
Mva1269I GAATGC 2 cut(s) 376, 834
MvaI CCWGG 1 cut(s) 37
MwoI GCNNNNNNNGC 3 cut(s) 417, 797, 866
NciI CCSGG 2 cut(s) 313, 1006
NcoI CCATGG 1 cut(s) 476
NdeI CATATG 1 cut(s) 713
NdeII GATC 3 cut(s) 90, 192, 991
NlaIII CATG 4 cut(s) 222, 480, 764, 789
NlaIV GGNNCC 3 cut(s) 154, 427, 690
NmuCI GTSAC 1 cut(s) 808
NspI RCATGY 1 cut(s) 789
NspV TTCGAA 1 cut(s) 1085
PciI ACATGT 1 cut(s) 785
PcsI WCGNNNNNNNCGW 3 cut(s) 107, 943, 964
PctI GAATGC 2 cut(s) 376, 834
PfeI GAWTC 2 cut(s) 328, 605
PfoI TCCNGGA 1 cut(s) 1004
PkrI GCNGC 2 cut(s) 793, 1054
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PscI ACATGT 1 cut(s) 785
Psp124BI GAGCTC 1 cut(s) 932
Psp6I CCWGG 1 cut(s) 35
PspGI CCWGG 1 cut(s) 35
PspN4I GGNNCC 3 cut(s) 154, 427, 690
RsaI GTAC 1 cut(s) 727
RsaNI GTAC 1 cut(s) 726
SacI GAGCTC 1 cut(s) 932
SaqAI TTAA 3 cut(s) 14, 516, 755
SatI GCNGC 2 cut(s) 792, 1053
Sau3AI GATC 3 cut(s) 90, 192, 991
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 3 cut(s) 37, 313, 1006
SduI GDGCHC 3 cut(s) 155, 932, 1040
SetI ASST 9 cut(s) 186, 192, 232, 256, 289, 436, 544, 871, 932
SfaNI GCATC 7 cut(s) 540, 638, 809, 873, 933, 938, 1072
SfcI CTRYAG 2 cut(s) 414, 535
SfuI TTCGAA 1 cut(s) 1085
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 7 cut(s) 10, 15, 135, 486, 804, 848, 903
SsiI CCGC 1 cut(s) 453
SspMI CTAG 1 cut(s) 281
SstI GAGCTC 1 cut(s) 932
StyD4I CCNGG 3 cut(s) 35, 311, 1004
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 2 cut(s) 157, 1042
TaiI ACGT 1 cut(s) 544
TaqI TCGA 4 cut(s) 89, 641, 958, 1085
TasI AATT 7 cut(s) 10, 15, 135, 486, 804, 848, 903
TfiI GAWTC 2 cut(s) 328, 605
Tru1I TTAA 3 cut(s) 14, 516, 755
Tru9I TTAA 3 cut(s) 14, 516, 755
TscAI CASTG 2 cut(s) 885, 1045
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 2 cut(s) 791, 1052
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 3 cut(s) 383, 458, 588
TspGWI ACGGA 1 cut(s) 1004
TspRI CASTG 2 cut(s) 885, 1045
XapI RAATTY 1 cut(s) 135
XceI RCATGY 1 cut(s) 789
XmiI GTMKAC 1 cut(s) 782
XspI CTAG 1 cut(s) 281
ZraI GACGTC 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.