pycom08g06320

CSC1-like protein RXW8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003874
Physical Location & Seq
Reverse (-)
4884025 .. 4887212
3188 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1404 bp
ATGAATCTGGCAGCTCTTTTGACTTCTGCTGGTATTAATATAGCTGTGTGCGTGGTACTCTTTTTGCTATATTCTATATTGAGGAAACAACCGAGCAATGTGAACGTCTACTTTGGACGAATGCTTACGATATCCAAAAGAAGAAGTGATCTTTTCAATTTGGAAAGGTTTGTTCCTTCTGCTAGTTGGCTAGTGAAAGCATGGAGAACAAAAGAAGAAGATTTTTTGACAGTCGGTGGCTTGGATTCGGTTGTTTTTATCAGGATGCTTATTTTTAGTCTTCGAATCTTTTCTATTGCTGCTGTCATCTGCGTCTTTATAGTGCTTCCGGTGAATTATCATGGGCAAACGAAGGATCACCCGGGAATCCATGATGAATCCTTGGAAGTCTTTACCATCTTGAACGTTCATGAAGGTTCAAAGTGGCTTTGGACCCATTGTCTCGCATTGTACATTATCACTACTTCAGCTTGTATTCTCCTATACTTTGAATATAATCACGTCACTAGACTGAGGCTGGCATATATTATTGGATCTTCTTCAAATCCAAGTCATTTTACAATTGTTGTTCGTGCTATTCCATGGTCACCAGAAGGATCTTATAGTGATTCAGTAAGAAAATTCTTCACAAAATATCATGCATCAAGCTACTTGTCACACCAAATGGTGTATCGATCTGGTAAAGTTCAGAAACTGATGACCGATGCAGGGAAGATGTGCAAGATTCTTAAGGATGTTTCTGTGGAACAGAGTCATAGACCAGATCTATTTCATTGTGGTATGTCTGGAGGAGCTACTGATTCTTTTAAGATACTCACGAACGATTCAGAGAGTGTTAAAGGGAAGTCATCCCTTATCGACAATAGTTTGGGTGGAAGAAAAAAGGTGTGTGCTGCTGCTTTTGTCTTTTTCAAGACTCGCTGTGCTGCTCTTGTTACATCAAAGGTTCTTCATTCTTCAAATCCTATGCATTGGGTGACAGATATGGCTCCGGAACCAAATGATGTGTATTGGTCAAACCTTTGGATACCGTACGGACAACTGTGGATCCGGAAGTTAGCCACACTTCTGGCTACTATTGCCTTTATGCTTGTGTTTCTTATTCCTGTCACATTTGTTCAGGGCATGACTGCACTAAAAAGCCTGGAAAAAAAGTTTCCAGCTTTGAAAGGATTGCTCAAAAAGAACTATGTAAGCCAGGTGATAACGGGGTACCTGCCAAGTGTGGTTTTAATTGTGGCTTTTTACATCGTTCCACCTGTCATGATGCTGTTTTCAACAGTGGAGGGGTGGATCGATCTCGGAGTCAGAGGAAAAAGAGTGCATGCTTCAAAGTCTTATACTTCACAATCTGGAATGTCTTTTTTGTTAATGTTTTTACCGGCTCTGTTATCAGCCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

468

Amino Acids

52.4

Weight (kDa)

9.58

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 164 2.1e-37 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 185 - 340 8.8e-26 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 351 - 433 5.6e-16 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1224
Acc65I GGTACC 1 cut(s) 1212
AccB1I GGYRCC 1 cut(s) 1212
AccI GTMKAC 1 cut(s) 108
AccIII TCCGGA 2 cut(s) 991, 1050
AclI AACGTT 1 cut(s) 405
AclWI GGATC 6 cut(s) 363, 541, 604, 1042, 1055, 1301
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 1 cut(s) 450
AfaI GTAC 4 cut(s) 57, 452, 1034, 1214
AfiI CCNNNNNNNGG 1 cut(s) 708
AflII CTTAAG 1 cut(s) 728
AhdI GACNNNNNGTC 1 cut(s) 438
AjiI CACGTC 1 cut(s) 502
AjnI CCWGG 2 cut(s) 1143, 1197
AluBI AGCT 6 cut(s) 14, 44, 470, 648, 794, 1163
AluI AGCT 6 cut(s) 14, 44, 470, 648, 794, 1163
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 6 cut(s) 363, 541, 604, 1042, 1055, 1301
AlwNI CAGNNNCTG 1 cut(s) 694
Ama87I CYCGRG 1 cut(s) 361
Aor13HI TCCGGA 2 cut(s) 991, 1050
ApeKI GCWGC 5 cut(s) 11, 299, 893, 896, 926
ApoI RAATTY 1 cut(s) 620
AseI ATTAAT 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 289
Asp718I GGTACC 1 cut(s) 1212
AspS9I GGNCC 1 cut(s) 432
AsuC2I CCSGG 2 cut(s) 362, 363
AsuHPI GGTGA 5 cut(s) 343, 350, 579, 988, 1213
AsuII TTCGAA 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 361
AvaII GGWCC 1 cut(s) 432
BamHI GGATCC 1 cut(s) 1047
BanI GGYRCC 1 cut(s) 1212
BbsI GAAGAC 1 cut(s) 272
BbvI GCAGC 5 cut(s) 23, 286, 880, 883, 913
BccI CCATC 1 cut(s) 404
BciT130I CCWGG 2 cut(s) 1145, 1199
BciVI GTATCC 1 cut(s) 1020
BcnI CCSGG 2 cut(s) 362, 363
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 3 cut(s) 183, 191, 507
BfrI CTTAAG 1 cut(s) 728
BfuAI ACCTGC 1 cut(s) 1224
BfuI GTATCC 1 cut(s) 1020
BglII AGATCT 1 cut(s) 763
BisI GCNGC 5 cut(s) 12, 300, 894, 897, 927
BlsI GCNGC 5 cut(s) 13, 301, 895, 898, 928
Bme1390I CCNGG 4 cut(s) 362, 363, 1145, 1199
Bme18I GGWCC 1 cut(s) 432
BmeRI GACNNNNNGTC 1 cut(s) 438
BmeT110I CYCGRG 1 cut(s) 361
BmgBI CACGTC 1 cut(s) 502
BmgT120I GGNCC 1 cut(s) 432
BmiI GGNNCC 5 cut(s) 434, 990, 996, 1049, 1214
BmrFI CCNGG 4 cut(s) 362, 363, 1145, 1199
BmsI GCATC 4 cut(s) 255, 650, 694, 1257
BpiI GAAGAC 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 807
Bpu14I TTCGAA 1 cut(s) 283
BpuMI CCSGG 2 cut(s) 362, 363
Bsa29I ATCGAT 2 cut(s) 673, 1296
BsaJI CCNNGG 3 cut(s) 361, 381, 581
BsaWI WCCGGW 3 cut(s) 328, 991, 1050
Bsc4I CCNNNNNNNGG 1 cut(s) 708
Bse118I RCCGGY 1 cut(s) 1381
Bse3DI GCAATG 1 cut(s) 103
BseAI TCCGGA 2 cut(s) 991, 1050
BseBI CCWGG 2 cut(s) 1145, 1199
BseCI ATCGAT 2 cut(s) 673, 1296
BseDI CCNNGG 3 cut(s) 361, 381, 581
BseGI GGATG 3 cut(s) 270, 739, 848
BseLI CCNNNNNNNGG 1 cut(s) 708
BseMI GCAATG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 503
BseRI GAGGAG 1 cut(s) 804
BseXI GCAGC 5 cut(s) 23, 286, 880, 883, 913
BsgI GTGCAG 1 cut(s) 1116
BshNI GGYRCC 1 cut(s) 1212
BshVI ATCGAT 2 cut(s) 673, 1296
BsiHKCI CYCGRG 1 cut(s) 361
BsiSI CCGG 5 cut(s) 329, 362, 992, 1051, 1382
BsiWI CGTACG 1 cut(s) 1032
BslI CCNNNNNNNGG 1 cut(s) 708
BsmAI GTCTC 1 cut(s) 446
BsmI GAATGC 1 cut(s) 126
BsoBI CYCGRG 1 cut(s) 361
Bsp119I TTCGAA 1 cut(s) 283
Bsp13I TCCGGA 2 cut(s) 991, 1050
Bsp1407I TGTACA 1 cut(s) 450
Bsp143I GATC 9 cut(s) 148, 355, 533, 596, 674, 763, 1047, 1293, 1297
Bsp19I CCATGG 1 cut(s) 581
BspCNI CTCAG 1 cut(s) 504
BspDI ATCGAT 2 cut(s) 673, 1296
BspEI TCCGGA 2 cut(s) 991, 1050
BspHI TCATGA 2 cut(s) 409, 1263
BspLI GGNNCC 5 cut(s) 434, 990, 996, 1049, 1214
BspMI ACCTGC 1 cut(s) 1224
BspPI GGATC 6 cut(s) 363, 541, 604, 1042, 1055, 1301
BspT104I TTCGAA 1 cut(s) 283
BspT107I GGYRCC 1 cut(s) 1212
BspTI CTTAAG 1 cut(s) 728
BsrDI GCAATG 1 cut(s) 103
BsrFI RCCGGY 1 cut(s) 1381
BsrGI TGTACA 1 cut(s) 450
BssAI RCCGGY 1 cut(s) 1381
BssECI CCNNGG 3 cut(s) 361, 381, 581
BssMI GATC 9 cut(s) 148, 355, 533, 596, 674, 763, 1047, 1293, 1297
BssT1I CCWWGG 2 cut(s) 381, 581
Bst2UI CCWGG 2 cut(s) 1145, 1199
Bst4CI ACNGT 4 cut(s) 232, 1032, 1044, 1282
BstAFI CTTAAG 1 cut(s) 728
BstAUI TGTACA 1 cut(s) 450
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 2 cut(s) 519, 1326
BstDEI CTNAG 1 cut(s) 512
BstDSI CCRYGG 1 cut(s) 581
BstEII GGTNACC 1 cut(s) 585
BstF5I GGATG 3 cut(s) 270, 739, 848
BstKTI GATC 9 cut(s) 151, 358, 536, 599, 677, 766, 1050, 1296, 1300
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 9 cut(s) 148, 355, 533, 596, 674, 763, 1047, 1293, 1297
BstMWI GCNNNNNNNGC 1 cut(s) 1079
BstNI CCWGG 2 cut(s) 1145, 1199
BstNSI RCATGY 1 cut(s) 1328
BstPI GGTNACC 1 cut(s) 585
BstSCI CCNGG 4 cut(s) 360, 361, 1143, 1197
BstV1I GCAGC 5 cut(s) 23, 286, 880, 883, 913
BstV2I GAAGAC 1 cut(s) 272
BstX2I RGATCY 4 cut(s) 533, 596, 763, 1047
BstXI CCANNNNNNTGG 1 cut(s) 1069
BstYI RGATCY 4 cut(s) 533, 596, 763, 1047
Bsu15I ATCGAT 2 cut(s) 673, 1296
BsuI GTATCC 1 cut(s) 1020
BsuTUI ATCGAT 2 cut(s) 673, 1296
BtgI CCRYGG 1 cut(s) 581
BtrI CACGTC 1 cut(s) 502
BtsCI GGATG 3 cut(s) 270, 739, 848
BtsIMutI CAGTG 1 cut(s) 1287
BveI ACCTGC 1 cut(s) 1224
Cac8I GCNNGC 2 cut(s) 519, 1326
CaiI CAGNNNCTG 1 cut(s) 694
CciI TCATGA 2 cut(s) 409, 1263
Cfr10I RCCGGY 1 cut(s) 1381
Cfr13I GGNCC 1 cut(s) 432
Cfr9I CCCGGG 1 cut(s) 361
ClaI ATCGAT 2 cut(s) 673, 1296
CseI GACGC 1 cut(s) 301
Csp6I GTAC 4 cut(s) 56, 451, 1033, 1213
CviAII CATG 9 cut(s) 201, 341, 371, 410, 582, 638, 1126, 1264, 1325
CviQI GTAC 4 cut(s) 56, 451, 1033, 1213
DdeI CTNAG 1 cut(s) 512
DpnI GATC 9 cut(s) 150, 357, 535, 598, 676, 765, 1049, 1295, 1299
DpnII GATC 9 cut(s) 148, 355, 533, 596, 674, 763, 1047, 1293, 1297
DriI GACNNNNNGTC 1 cut(s) 438
Eam1105I GACNNNNNGTC 1 cut(s) 438
Eco130I CCWWGG 2 cut(s) 381, 581
Eco32I GATATC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 432
Eco57I CTGAAG 1 cut(s) 450
Eco88I CYCGRG 1 cut(s) 361
Eco91I GGTNACC 1 cut(s) 585
EcoO65I GGTNACC 1 cut(s) 585
EcoRII CCWGG 2 cut(s) 1143, 1197
EcoRV GATATC 1 cut(s) 132
EcoT14I CCWWGG 2 cut(s) 381, 581
EcoT22I ATGCAT 2 cut(s) 643, 972
ErhI CCWWGG 2 cut(s) 381, 581
FaeI CATG 9 cut(s) 204, 344, 374, 413, 585, 641, 1129, 1267, 1328
FatI CATG 9 cut(s) 200, 340, 370, 409, 581, 637, 1125, 1263, 1324
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 5 cut(s) 12, 300, 894, 897, 927
FokI GGATG 3 cut(s) 277, 746, 835
Fsp4HI GCNGC 5 cut(s) 12, 300, 894, 897, 927
FspBI CTAG 3 cut(s) 183, 191, 507
GluI GCNGC 5 cut(s) 12, 300, 894, 897, 927
GsuI CTGGAG 1 cut(s) 807
HapII CCGG 5 cut(s) 329, 362, 992, 1051, 1382
HgaI GACGC 1 cut(s) 301
Hin1II CATG 9 cut(s) 204, 344, 374, 413, 585, 641, 1129, 1267, 1328
HpaII CCGG 5 cut(s) 329, 362, 992, 1051, 1382
HphI GGTGA 5 cut(s) 343, 350, 579, 988, 1213
Hpy166II GTNNAC 2 cut(s) 103, 109
Hpy188I TCNGA 4 cut(s) 690, 829, 1304, 1310
Hpy8I GTNNAC 2 cut(s) 103, 109
HpyAV CCTTC 4 cut(s) 186, 346, 407, 587
HpyCH4III ACNGT 4 cut(s) 232, 1032, 1044, 1282
HpyCH4IV ACGT 3 cut(s) 105, 405, 501
HpyCH4V TGCA 6 cut(s) 641, 707, 720, 970, 1133, 1324
HpyF10VI GCNNNNNNNGC 1 cut(s) 1079
HpyF3I CTNAG 1 cut(s) 512
HpySE526I ACGT 3 cut(s) 105, 405, 501
Hsp92II CATG 9 cut(s) 204, 344, 374, 413, 585, 641, 1129, 1267, 1328
Kpn2I TCCGGA 2 cut(s) 991, 1050
KpnI GGTACC 1 cut(s) 1216
Kzo9I GATC 9 cut(s) 148, 355, 533, 596, 674, 763, 1047, 1293, 1297
LmnI GCTCC 2 cut(s) 791, 994
Lsp1109I GCAGC 5 cut(s) 23, 286, 880, 883, 913
LweI GCATC 4 cut(s) 255, 650, 694, 1257
MaeI CTAG 3 cut(s) 183, 191, 507
MaeII ACGT 3 cut(s) 105, 405, 501
MaeIII GTNAC 6 cut(s) 502, 585, 654, 934, 976, 1108
MalI GATC 9 cut(s) 150, 357, 535, 598, 676, 765, 1049, 1295, 1299
MboI GATC 9 cut(s) 148, 355, 533, 596, 674, 763, 1047, 1293, 1297
MfeI CAATTG 2 cut(s) 561, 1399
MflI RGATCY 4 cut(s) 533, 596, 763, 1047
MluCI AATT 6 cut(s) 157, 334, 561, 620, 1233, 1399
MlyI GAGTC 3 cut(s) 760, 910, 1314
MnlI CCTC 5 cut(s) 75, 507, 782, 1279, 1304
Mph1103I ATGCAT 2 cut(s) 643, 972
MroI TCCGGA 2 cut(s) 991, 1050
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 6 cut(s) 36, 729, 807, 837, 1232, 1370
MspCI CTTAAG 1 cut(s) 728
MspI CCGG 5 cut(s) 329, 362, 992, 1051, 1382
MspR9I CCNGG 4 cut(s) 362, 363, 1145, 1199
MunI CAATTG 2 cut(s) 561, 1399
Mva1269I GAATGC 1 cut(s) 126
MvaI CCWGG 2 cut(s) 1145, 1199
MwoI GCNNNNNNNGC 1 cut(s) 1079
NciI CCSGG 2 cut(s) 362, 363
NcoI CCATGG 1 cut(s) 581
NdeII GATC 9 cut(s) 148, 355, 533, 596, 674, 763, 1047, 1293, 1297
NlaIII CATG 9 cut(s) 204, 344, 374, 413, 585, 641, 1129, 1267, 1328
NlaIV GGNNCC 5 cut(s) 434, 990, 996, 1049, 1214
NmuCI GTSAC 5 cut(s) 502, 585, 654, 976, 1108
NsiI ATGCAT 2 cut(s) 643, 972
NspI RCATGY 1 cut(s) 1328
NspV TTCGAA 1 cut(s) 283
PaeI GCATGC 1 cut(s) 1328
PagI TCATGA 2 cut(s) 409, 1263
PctI GAATGC 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 9 cut(s) 4, 245, 285, 366, 377, 608, 724, 800, 824
Pfl23II CGTACG 1 cut(s) 1032
PkrI GCNGC 5 cut(s) 13, 301, 895, 898, 928
PleI GAGTC 3 cut(s) 759, 910, 1313
PpsI GAGTC 3 cut(s) 759, 910, 1313
PshBI ATTAAT 1 cut(s) 36
Psp1406I AACGTT 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 1143, 1197
PspEI GGTNACC 1 cut(s) 585
PspGI CCWGG 2 cut(s) 1143, 1197
PspLI CGTACG 1 cut(s) 1032
PspN4I GGNNCC 5 cut(s) 434, 990, 996, 1049, 1214
PspPI GGNCC 1 cut(s) 432
PstNI CAGNNNCTG 1 cut(s) 694
PsuI RGATCY 4 cut(s) 533, 596, 763, 1047
RsaI GTAC 4 cut(s) 57, 452, 1034, 1214
RsaNI GTAC 4 cut(s) 56, 451, 1033, 1213
SaqAI TTAA 6 cut(s) 36, 729, 807, 837, 1232, 1370
SatI GCNGC 5 cut(s) 12, 300, 894, 897, 927
Sau3AI GATC 9 cut(s) 148, 355, 533, 596, 674, 763, 1047, 1293, 1297
Sau96I GGNCC 1 cut(s) 432
SchI GAGTC 3 cut(s) 760, 910, 1314
ScrFI CCNGG 4 cut(s) 362, 363, 1145, 1199
SfaNI GCATC 4 cut(s) 255, 650, 694, 1257
SfuI TTCGAA 1 cut(s) 283
SinI GGWCC 1 cut(s) 432
SmaI CCCGGG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
SphI GCATGC 1 cut(s) 1328
Sse9I AATT 6 cut(s) 157, 334, 561, 620, 1233, 1399
SspMI CTAG 3 cut(s) 183, 191, 507
StyD4I CCNGG 4 cut(s) 360, 361, 1143, 1197
StyI CCWWGG 2 cut(s) 381, 581
TaaI ACNGT 4 cut(s) 232, 1032, 1044, 1282
TaiI ACGT 3 cut(s) 108, 408, 504
TaqI TCGA 4 cut(s) 283, 673, 858, 1296
TaqII GACCGA 1 cut(s) 716
TasI AATT 6 cut(s) 157, 334, 561, 620, 1233, 1399
TatI WGTACW 1 cut(s) 450
TfiI GAWTC 9 cut(s) 4, 245, 285, 366, 377, 608, 724, 800, 824
Tru1I TTAA 6 cut(s) 36, 729, 807, 837, 1232, 1370
Tru9I TTAA 6 cut(s) 36, 729, 807, 837, 1232, 1370
TscAI CASTG 1 cut(s) 1287
TseFI GTSAC 5 cut(s) 502, 585, 654, 976, 1108
TseI GCWGC 5 cut(s) 11, 299, 893, 896, 926
Tsp45I GTSAC 5 cut(s) 502, 585, 654, 976, 1108
TspDTI ATGAA 6 cut(s) 17, 390, 398, 426, 761, 941
TspGWI ACGGA 1 cut(s) 1050
TspMI CCCGGG 1 cut(s) 361
TspRI CASTG 1 cut(s) 1287
Vha464I CTTAAG 1 cut(s) 728
VpaK11BI GGWCC 1 cut(s) 432
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 1 cut(s) 620
XceI RCATGY 1 cut(s) 1328
XmaI CCCGGG 1 cut(s) 361
XmiI GTMKAC 1 cut(s) 108
XmnI GAANNNNTTC 1 cut(s) 289
XspI CTAG 3 cut(s) 183, 191, 507
Zsp2I ATGCAT 2 cut(s) 643, 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.