pycom08g11640

TIFY 8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008601
Physical Location & Seq
Forward (+)
9880105 .. 9884600
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1338 bp
ATGGCTGTTCTGAAAATGGCCCAGCAAGGCATCCCCAACAACAATGCCACAACTCTTAGCGGCCAACAGCAAAAACAGCAGCCGGGGAAGCAGAAGCTAGAGGAGCAGCAGCTGGGGAAGCACACCATCTTCCATGACTTTCTGGGCATGAAGCCTGGAGATTCCCCTGTGCTTTTGCCAGCAAAAGCCTCCACCGATTGCAGGCTCTCTGAGCCTTCTCCCTCGGCCTCCGCTTCCCTTGGGGCTTCCTCCGGCGGCGGCCGGGGCCCCATTTCTACCACCTCTGATCTGGGTTCCGAAAGGCACGCTGGAAACCATCTTGAGGGGGTTCCGTTCTATGGCCATAGGAGCGATGTATCTGGGCTTGAGATAAGTAACAGGGTACTAGGAAGTAAACGGAGTAATTCAGACTCGACTTTTATGGGTTCATCCAGAGATGGTGTCCCTCATACCGGTCCTGATCACCTTGAGAGCTCGCATTTGATGAAGATGCTTCGAAATGGACCAGGGGGAGAACGACCTAGAATGTCCAATGACGATGAAGCAGTCTTTGCTACACAGCCACCGAGGCCAACTTCTGGTTCTCTGATTTTTCAGCCTCCACTTGGTGGTAGAGTTGACACCAAATGGGAACGACCTAGTCCTATGAATATGGGTGCTGCAGTACAGTACCCTTCACGAGGGGGTCATTTTGTACCCATGGTGCATCAATTACCTTCAAACCGATTTAGGGATGCCAATGCCATTCCAAACAATATCTCTCAATCAGCAGCAGATGAGGGATCTAGAACTGGAATTAAAGGCCCGGGAATTTTGAGTTCCATTAATGCCAGCAGTGATGCTCCTGAAAGAAACTCGTCTGGGGTGCTACCAAGTGGCAGCAGGCAGAAATCTGGGACTCATAATTTAGAGTCAGAACCTTCTAATCCAAGTCAACATGGATTTACTTCAGCTGGTCGGCAGATGACCATATTTTATGGTGGTCAAGCTCATGTTTTTGACAATGTCCATCCGAACAAGGCAGATGTTATAATGGCTTTAGCTGGATCAAATGGAGGATCTTGGTCAACAACCTACTCACTAAAACCTAATGCAAAGCTGGGCAGTGAAAGTTGCATGCCTAGTGGAGAGGTTGAAACCGGTGCAGCAAGTAACAACGCACTGTTGCGAGAATACCATGGGAGGTTAAGTGTTCCTGGGAATTCTAGTCCAGCTGCGGGTTTTGCTGATCGAATATTGACACCAGCAGGTGGCCATCAAGGTAGCAATTTAGTCAGAGATACAAGAAACCTGATTCAAGCTGCGGAACCCAGTTCAAAAGAGAAAAATGAGCTGTGA

Protein Analysis

446

Amino Acids

46.83

Weight (kDa)

8.79

Isoelectric Point (pI)

53.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tify PF06200 318 - 349 2.2e-14 tify domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1031
AarI CACCTGC 1 cut(s) 1238
Acc36I ACCTGC 1 cut(s) 1238
AccB7I CCANNNNNTGG 3 cut(s) 578, 608, 1250
AciI CCGC 6 cut(s) 60, 231, 255, 258, 1217, 1304
AclWI GGATC 3 cut(s) 790, 1054, 1066
AcoI YGGCCR 4 cut(s) 61, 259, 340, 1252
AcsI RAATTY 2 cut(s) 810, 1201
AcuI CTGAAG 1 cut(s) 933
AdeI CACNNNGTG 1 cut(s) 608
AfaI GTAC 4 cut(s) 384, 666, 671, 696
AgeI ACCGGT 2 cut(s) 452, 1139
AgsI TTSAA 4 cut(s) 720, 1136, 1298, 1317
AjnI CCWGG 3 cut(s) 154, 505, 1195
AjuI GAANNNNNNNTTGG 2 cut(s) 565, 597
Alw21I GWGCWC 1 cut(s) 476
AlwI GGATC 3 cut(s) 790, 1054, 1066
AlwNI CAGNNNCTG 1 cut(s) 112
Ama87I CYCGRG 1 cut(s) 805
AoxI GGCC 9 cut(s) 18, 61, 225, 259, 265, 340, 569, 802, 1252
ApaI GGGCCC 1 cut(s) 269
ApeKI GCWGC 9 cut(s) 79, 106, 109, 659, 770, 879, 1145, 1214, 1301
ApoI RAATTY 2 cut(s) 810, 1201
AseI ATTAAT 1 cut(s) 825
AsiGI ACCGGT 2 cut(s) 452, 1139
AspS9I GGNCC 6 cut(s) 19, 265, 266, 455, 503, 803
AsuC2I CCSGG 4 cut(s) 84, 263, 806, 807
AsuHPI GGTGA 1 cut(s) 455
AsuII TTCGAA 1 cut(s) 496
AvaI CYCGRG 1 cut(s) 805
AvaII GGWCC 2 cut(s) 455, 503
BaeGI GKGCMC 1 cut(s) 269
BalI TGGCCA 2 cut(s) 342, 1254
BanII GRGCYC 2 cut(s) 269, 476
BauI CACGAG 1 cut(s) 678
Bbv12I GWGCWC 1 cut(s) 476
BbvI GCAGC 9 cut(s) 91, 118, 121, 646, 782, 891, 1157, 1201, 1288
BccI CCATC 5 cut(s) 134, 324, 431, 1017, 1263
BciT130I CCWGG 3 cut(s) 156, 507, 1197
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 4 cut(s) 84, 263, 806, 807
BfaI CTAG 7 cut(s) 98, 386, 522, 639, 786, 1122, 1206
BfmI CTRYAG 1 cut(s) 660
BfuAI ACCTGC 1 cut(s) 1238
BglI GCCNNNNNGGC 1 cut(s) 568
Bme1390I CCNGG 7 cut(s) 84, 156, 263, 507, 806, 807, 1197
Bme18I GGWCC 2 cut(s) 455, 503
BmeT110I CYCGRG 1 cut(s) 805
BmgT120I GGNCC 6 cut(s) 19, 265, 266, 455, 503, 803
BmiI GGNNCC 6 cut(s) 266, 267, 268, 295, 330, 1308
BmrFI CCNGG 7 cut(s) 84, 156, 263, 507, 806, 807, 1197
BmrI ACTGGG 1 cut(s) 1305
BmsI GCATC 5 cut(s) 39, 480, 715, 724, 829
BmuI ACTGGG 1 cut(s) 1305
BpmI CTGGAG 1 cut(s) 177
Bpu14I TTCGAA 1 cut(s) 496
BpuEI CTTGAG 3 cut(s) 341, 386, 488
BpuMI CCSGG 4 cut(s) 84, 263, 806, 807
BsaWI WCCGGW 2 cut(s) 452, 1139
BsaXI ACNNNNNCTCC 2 cut(s) 1174, 1204
Bse118I RCCGGY 2 cut(s) 452, 1139
Bse1I ACTGG 2 cut(s) 796, 1311
BseBI CCWGG 3 cut(s) 156, 507, 1197
BseGI GGATG 4 cut(s) 30, 428, 739, 1009
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 2 cut(s) 796, 1311
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 269
BseX3I CGGCCG 1 cut(s) 259
BseXI GCAGC 9 cut(s) 91, 118, 121, 646, 782, 891, 1157, 1201, 1288
BseYI CCCAGC 3 cut(s) 21, 112, 1099
BsgI GTGCAG 1 cut(s) 1164
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 9 cut(s) 20, 63, 227, 261, 267, 342, 571, 804, 1254
BshTI ACCGGT 2 cut(s) 452, 1139
BsiEI CGRYCG 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 476
BsiHKCI CYCGRG 1 cut(s) 805
BsiSI CCGG 6 cut(s) 83, 252, 262, 453, 806, 1140
BslFI GGGAC 2 cut(s) 428, 910
BsmFI GGGAC 2 cut(s) 428, 910
BsnI GGCC 9 cut(s) 20, 63, 227, 261, 267, 342, 571, 804, 1254
BsoBI CYCGRG 1 cut(s) 805
Bsp119I TTCGAA 1 cut(s) 496
Bsp120I GGGCCC 1 cut(s) 265
Bsp1286I GDGCHC 2 cut(s) 269, 476
Bsp143I GATC 6 cut(s) 286, 460, 782, 1046, 1058, 1228
Bsp19I CCATGG 2 cut(s) 699, 1177
BspACI CCGC 6 cut(s) 60, 231, 255, 258, 1217, 1304
BspANI GGCC 9 cut(s) 20, 63, 227, 261, 267, 342, 571, 804, 1254
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 6 cut(s) 266, 267, 268, 295, 330, 1308
BspMAI CTGCAG 1 cut(s) 664
BspMI ACCTGC 1 cut(s) 1238
BspPI GGATC 3 cut(s) 790, 1054, 1066
BspT104I TTCGAA 1 cut(s) 496
BsrFI RCCGGY 2 cut(s) 452, 1139
BsrI ACTGG 2 cut(s) 796, 1311
BssAI RCCGGY 2 cut(s) 452, 1139
BssMI GATC 6 cut(s) 286, 460, 782, 1046, 1058, 1228
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 3 cut(s) 238, 699, 1177
Bst2BI CACGAG 1 cut(s) 678
Bst2UI CCWGG 3 cut(s) 156, 507, 1197
Bst4CI ACNGT 2 cut(s) 669, 1164
BstAPI GCANNNNNTGC 1 cut(s) 551
BstBI TTCGAA 1 cut(s) 496
BstC8I GCNNGC 7 cut(s) 180, 203, 306, 476, 832, 884, 1118
BstDEI CTNAG 2 cut(s) 56, 210
BstDSI CCRYGG 2 cut(s) 699, 1177
BstENI CCTNNNNNAGG 1 cut(s) 678
BstF5I GGATG 4 cut(s) 30, 428, 739, 1009
BstKTI GATC 6 cut(s) 289, 463, 785, 1049, 1061, 1231
BstMBI GATC 6 cut(s) 286, 460, 782, 1046, 1058, 1228
BstMCI CGRYCG 1 cut(s) 262
BstNI CCWGG 3 cut(s) 156, 507, 1197
BstNSI RCATGY 1 cut(s) 1120
BstSCI CCNGG 7 cut(s) 82, 154, 261, 505, 804, 805, 1195
BstSFI CTRYAG 1 cut(s) 660
BstSLI GKGCMC 1 cut(s) 269
BstV1I GCAGC 9 cut(s) 91, 118, 121, 646, 782, 891, 1157, 1201, 1288
BstX2I RGATCY 2 cut(s) 782, 1058
BstYI RGATCY 2 cut(s) 782, 1058
BstZI CGGCCG 1 cut(s) 259
BsuRI GGCC 9 cut(s) 20, 63, 227, 261, 267, 342, 571, 804, 1254
BtgI CCRYGG 2 cut(s) 699, 1177
BtgZI GCGATG 1 cut(s) 366
BtsCI GGATG 4 cut(s) 30, 428, 739, 1009
BtsI GCAGTG 2 cut(s) 841, 1111
BtsIMutI CAGTG 3 cut(s) 841, 1111, 1160
BveI ACCTGC 1 cut(s) 1238
Cac8I GCNNGC 7 cut(s) 180, 203, 306, 476, 832, 884, 1118
CaiI CAGNNNCTG 1 cut(s) 112
Cfr10I RCCGGY 2 cut(s) 452, 1139
Cfr13I GGNCC 6 cut(s) 19, 265, 266, 455, 503, 803
Cfr9I CCCGGG 1 cut(s) 805
Csp6I GTAC 4 cut(s) 383, 665, 670, 695
CspAI ACCGGT 2 cut(s) 452, 1139
CviAII CATG 7 cut(s) 134, 148, 700, 938, 992, 1117, 1178
CviQI GTAC 4 cut(s) 383, 665, 670, 695
DdeI CTNAG 2 cut(s) 56, 210
DpnI GATC 6 cut(s) 288, 462, 784, 1048, 1060, 1230
DpnII GATC 6 cut(s) 286, 460, 782, 1046, 1058, 1228
DraIII CACNNNGTG 1 cut(s) 608
EaeI YGGCCR 4 cut(s) 61, 259, 340, 1252
EagI CGGCCG 1 cut(s) 259
Ecl136II GAGCTC 1 cut(s) 474
EclXI CGGCCG 1 cut(s) 259
Eco130I CCWWGG 3 cut(s) 238, 699, 1177
Eco24I GRGCYC 2 cut(s) 269, 476
Eco47I GGWCC 2 cut(s) 455, 503
Eco52I CGGCCG 1 cut(s) 259
Eco53kI GAGCTC 1 cut(s) 474
Eco57I CTGAAG 1 cut(s) 933
Eco88I CYCGRG 1 cut(s) 805
EcoICRI GAGCTC 1 cut(s) 474
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO109I RGGNCCY 2 cut(s) 265, 266
EcoRI GAATTC 1 cut(s) 1201
EcoRII CCWGG 3 cut(s) 154, 505, 1195
EcoT14I CCWWGG 3 cut(s) 238, 699, 1177
EcoT38I GRGCYC 2 cut(s) 269, 476
ErhI CCWWGG 3 cut(s) 238, 699, 1177
FaeI CATG 7 cut(s) 137, 151, 703, 941, 995, 1120, 1181
FaqI GGGAC 2 cut(s) 428, 910
FatI CATG 7 cut(s) 133, 147, 699, 937, 991, 1116, 1177
FauI CCCGC 1 cut(s) 1210
FbaI TGATCA 1 cut(s) 460
FokI GGATG 4 cut(s) 17, 415, 746, 996
FriOI GRGCYC 2 cut(s) 269, 476
FspBI CTAG 7 cut(s) 98, 386, 522, 639, 786, 1122, 1206
GsaI CCCAGC 3 cut(s) 25, 116, 1103
GsuI CTGGAG 1 cut(s) 177
HaeIII GGCC 9 cut(s) 20, 63, 227, 261, 267, 342, 571, 804, 1254
HapII CCGG 6 cut(s) 83, 252, 262, 453, 806, 1140
Hin1II CATG 7 cut(s) 137, 151, 703, 941, 995, 1120, 1181
HincII GTYRAC 3 cut(s) 619, 935, 1068
HindII GTYRAC 3 cut(s) 619, 935, 1068
HinfI GANTC 5 cut(s) 161, 410, 898, 911, 1294
HpaII CCGG 6 cut(s) 83, 252, 262, 453, 806, 1140
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 4 cut(s) 395, 619, 935, 1068
Hpy188I TCNGA 9 cut(s) 12, 211, 286, 298, 409, 588, 916, 1014, 1277
Hpy188III TCNNGA 6 cut(s) 320, 432, 458, 678, 786, 845
Hpy8I GTNNAC 4 cut(s) 395, 619, 935, 1068
HpyAV CCTTC 4 cut(s) 225, 684, 726, 930
HpyCH4III ACNGT 2 cut(s) 669, 1164
HpyCH4V TGCA 6 cut(s) 201, 662, 706, 1094, 1116, 1145
HpyF3I CTNAG 2 cut(s) 56, 210
Hsp92II CATG 7 cut(s) 137, 151, 703, 941, 995, 1120, 1181
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 6 cut(s) 286, 460, 782, 1046, 1058, 1228
LmnI GCTCC 3 cut(s) 103, 348, 847
Lsp1109I GCAGC 9 cut(s) 91, 118, 121, 646, 782, 891, 1157, 1201, 1288
LweI GCATC 5 cut(s) 39, 480, 715, 724, 829
MaeI CTAG 7 cut(s) 98, 386, 522, 639, 786, 1122, 1206
MaeIII GTNAC 2 cut(s) 374, 1151
MalI GATC 6 cut(s) 288, 462, 784, 1048, 1060, 1230
MboI GATC 6 cut(s) 286, 460, 782, 1046, 1058, 1228
MboII GAAGA 2 cut(s) 121, 499
MflI RGATCY 2 cut(s) 782, 1058
MhlI GDGCHC 2 cut(s) 269, 476
MlsI TGGCCA 2 cut(s) 342, 1254
MluCI AATT 7 cut(s) 403, 710, 795, 810, 904, 1201, 1267
MluNI TGGCCA 2 cut(s) 342, 1254
MlyI GAGTC 3 cut(s) 404, 892, 920
Mox20I TGGCCA 2 cut(s) 342, 1254
MscI TGGCCA 2 cut(s) 342, 1254
MseI TTAA 3 cut(s) 798, 825, 1187
Msp20I TGGCCA 2 cut(s) 342, 1254
MspA1I CMGCKG 3 cut(s) 112, 953, 1214
MspI CCGG 6 cut(s) 83, 252, 262, 453, 806, 1140
MspR9I CCNGG 7 cut(s) 84, 156, 263, 507, 806, 807, 1197
MvaI CCWGG 3 cut(s) 156, 507, 1197
NciI CCSGG 4 cut(s) 84, 263, 806, 807
NcoI CCATGG 2 cut(s) 699, 1177
NdeII GATC 6 cut(s) 286, 460, 782, 1046, 1058, 1228
NlaIII CATG 7 cut(s) 137, 151, 703, 941, 995, 1120, 1181
NlaIV GGNNCC 6 cut(s) 266, 267, 268, 295, 330, 1308
NmeAIII GCCGAG 1 cut(s) 203
NspI RCATGY 1 cut(s) 1120
NspV TTCGAA 1 cut(s) 496
PaeI GCATGC 1 cut(s) 1120
PaqCI CACCTGC 1 cut(s) 1238
PfeI GAWTC 2 cut(s) 161, 1294
PflFI GACNNNGTC 2 cut(s) 639, 1004
PflMI CCANNNNNTGG 3 cut(s) 578, 608, 1250
PinAI ACCGGT 2 cut(s) 452, 1139
PleI GAGTC 3 cut(s) 404, 892, 919
PpsI GAGTC 3 cut(s) 404, 892, 919
PshBI ATTAAT 1 cut(s) 825
PsiI TTATAA 1 cut(s) 1031
Psp124BI GAGCTC 1 cut(s) 476
Psp6I CCWGG 3 cut(s) 154, 505, 1195
PspFI CCCAGC 3 cut(s) 21, 112, 1099
PspGI CCWGG 3 cut(s) 154, 505, 1195
PspN4I GGNNCC 6 cut(s) 266, 267, 268, 295, 330, 1308
PspOMI GGGCCC 1 cut(s) 265
PspPI GGNCC 6 cut(s) 19, 265, 266, 455, 503, 803
PstI CTGCAG 1 cut(s) 664
PstNI CAGNNNCTG 1 cut(s) 112
PsuI RGATCY 2 cut(s) 782, 1058
PsyI GACNNNGTC 2 cut(s) 639, 1004
PvuII CAGCTG 3 cut(s) 112, 953, 1214
RsaI GTAC 4 cut(s) 384, 666, 671, 696
RsaNI GTAC 4 cut(s) 383, 665, 670, 695
SacI GAGCTC 1 cut(s) 476
SaqAI TTAA 3 cut(s) 798, 825, 1187
Sau3AI GATC 6 cut(s) 286, 460, 782, 1046, 1058, 1228
Sau96I GGNCC 6 cut(s) 19, 265, 266, 455, 503, 803
SchI GAGTC 3 cut(s) 404, 892, 920
ScrFI CCNGG 7 cut(s) 84, 156, 263, 507, 806, 807, 1197
SduI GDGCHC 2 cut(s) 269, 476
SfaNI GCATC 5 cut(s) 39, 480, 715, 724, 829
SfcI CTRYAG 1 cut(s) 660
SfuI TTCGAA 1 cut(s) 496
SinI GGWCC 2 cut(s) 455, 503
SmaI CCCGGG 1 cut(s) 807
SmlI CTYRAG 3 cut(s) 320, 365, 467
SmoI CTYRAG 3 cut(s) 320, 365, 467
SphI GCATGC 1 cut(s) 1120
Sse9I AATT 7 cut(s) 403, 710, 795, 810, 904, 1201, 1267
SsiI CCGC 6 cut(s) 60, 231, 255, 258, 1217, 1304
SspI AATATT 1 cut(s) 1236
SspMI CTAG 7 cut(s) 98, 386, 522, 639, 786, 1122, 1206
SstI GAGCTC 1 cut(s) 476
StyD4I CCNGG 7 cut(s) 82, 154, 261, 505, 804, 805, 1195
StyI CCWWGG 3 cut(s) 238, 699, 1177
TaaI ACNGT 2 cut(s) 669, 1164
TaqI TCGA 3 cut(s) 413, 496, 1231
TasI AATT 7 cut(s) 403, 710, 795, 810, 904, 1201, 1267
TatI WGTACW 1 cut(s) 664
TauI GCSGC 3 cut(s) 63, 258, 261
TfiI GAWTC 2 cut(s) 161, 1294
Tru1I TTAA 3 cut(s) 798, 825, 1187
Tru9I TTAA 3 cut(s) 798, 825, 1187
TscAI CASTG 3 cut(s) 841, 1111, 1167
TseI GCWGC 9 cut(s) 79, 106, 109, 659, 770, 879, 1145, 1214, 1301
TspDTI ATGAA 5 cut(s) 164, 417, 500, 555, 662
TspGWI ACGGA 2 cut(s) 321, 412
TspMI CCCGGG 1 cut(s) 805
TspRI CASTG 3 cut(s) 841, 1111, 1167
Tth111I GACNNNGTC 2 cut(s) 639, 1004
Van91I CCANNNNNTGG 3 cut(s) 578, 608, 1250
VpaK11BI GGWCC 2 cut(s) 455, 503
VspI ATTAAT 1 cut(s) 825
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 2 cut(s) 810, 1201
XbaI TCTAGA 1 cut(s) 785
XceI RCATGY 1 cut(s) 1120
XcmI CCANNNNNNNNNTGG 1 cut(s) 286
XmaI CCCGGG 1 cut(s) 805
XspI CTAG 7 cut(s) 98, 386, 522, 639, 786, 1122, 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.