Rh6AG517300

TIFY 8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0008601
Physical Location & Seq
Forward (+)
68663482 .. 68668493
5012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG517300.1

Sequence Viewer

Length: 1173 bp
ATGGCCGTACTGAAAATGGCTCTGCCCAGCAACAATGCCAGTACCCAAAAGCAGCAGAAGCTGAAGCAGGAAGAAGAAGACCAATTAGTGAAGCCAATGTTTCATGACTTTCTGGGTATGAAGCCATCTACTGATTCCCCTGTTGTTTTGGGCCCAAAAGGATCCGATTCCAGGCTCACTGAGGCATCTCCCTCCGCTTCTGCTTCACTTGGGGGATCCTCCGGCGGTGGCCGTGGGCCCATCTCCACCACCTCTGATCTGGGTTCCGAAAGACAAGCTGGAAATCATCTCGAGGGGGTTCCATATTATGGTCCAAGAAGCGATATTTCTGGGCCTGAGATAAGTAACAGGATACTAGGCAGTAAGAGAAGTAATTCAGACTCTACTTTTATGGGTTCATCCAGAGATGGGATCCCTCATATGGGTCCTGACTCCCTTGAGAGCTCACATTTGATGAAGATGTTTCGAAATGGAACTGGGGGAGAGCGACCTAGAAGGTCCATTGACGATGAAGCAGTCTTTGCTACCCAGCCAATGAGGCCAACTTCTGCTAATCTCATATTTCAGACTCCACATGGCGGTAGAGTTGATAACAATATTTCCAAGTGGGAACGATCTACTCCCATGAATTTGGGTGGTGCAGTGCAATACCCTCCACGAGGAGGTCATTTTGCACCCTTTGTTCATCAATTGCCTTCTAACAGATTTAGGGATGCAAATGCTGGTGCAAATAATATATCTCAATTAGCCGCTGATGAGGGGTCTAGAACTGGAATTAAAGGACCAGGGATATTGAGTGCCATCAATGCTAGCAGTGGTGCTTCTGATAGAAACTCATCTGTGGTGCTGCCTAGCGGCAGTAGGCAGAAATCTGGGACTAATACTTCAGAGCCAGAACCTTTGACTCCAAGTCGGCATGGATTTGCATCTGCTAACCGGCAGATGACCATATTTTATGGCGGTCAAGCTCATGTTTTCGATGATGTCCACCCAAACAAGGTTTTTCTCTCTTACTCTTTTGTTCTAGTAAAGAGAATGCCCCCTTTTATTTTCATTTATGGCGCTCATGTTTTCGATGATGTCCACCCAAACAAGGTTTCCCTCTCTTACTCTTCTATTCTAGTACAGAGAATGCCCCTTTTTATTTTCATTGTGTGTAAACAAGCTTTGTAA

Protein Analysis

390

Amino Acids

41.94

Weight (kDa)

9.46

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tify PF06200 310 - 334 2.4e-10 tify domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 308
AciI CCGC 6 cut(s) 195, 225, 579, 750, 855, 960
AclWI GGATC 6 cut(s) 156, 169, 210, 223, 406, 419
AcoI YGGCCR 2 cut(s) 3, 229
AcsI RAATTY 1 cut(s) 628
AcuI CTGAAG 2 cut(s) 83, 870
AfaI GTAC 3 cut(s) 9, 43, 1125
AhdI GACNNNNNGTC 1 cut(s) 909
AjnI CCWGG 2 cut(s) 170, 784
AjuI GAANNNNNNNTTGG 2 cut(s) 1081, 1113
AluBI AGCT 5 cut(s) 61, 278, 444, 968, 1166
AluI AGCT 5 cut(s) 61, 278, 444, 968, 1166
Alw21I GWGCWC 1 cut(s) 446
AlwI GGATC 6 cut(s) 156, 169, 210, 223, 406, 419
AlwNI CAGNNNCTG 1 cut(s) 61
Ama87I CYCGRG 1 cut(s) 290
AoxI GGCC 6 cut(s) 3, 151, 229, 236, 332, 539
ApaI GGGCCC 2 cut(s) 155, 240
ApeKI GCWGC 2 cut(s) 52, 847
ApoI RAATTY 1 cut(s) 628
Asp700I GAANNNNTTC 1 cut(s) 373
AspLEI GCGC 1 cut(s) 1064
AspS9I GGNCC 9 cut(s) 151, 152, 236, 237, 311, 332, 425, 498, 782
AsuII TTCGAA 1 cut(s) 466
AsuNHI GCTAGC 1 cut(s) 809
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 4 cut(s) 311, 425, 498, 782
BaeGI GKGCMC 2 cut(s) 155, 240
BamHI GGATCC 3 cut(s) 161, 215, 411
BanII GRGCYC 3 cut(s) 155, 240, 446
BauI CACGAG 1 cut(s) 657
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 446
BbvI GCAGC 2 cut(s) 64, 834
BccI CCATC 4 cut(s) 133, 248, 401, 809
BceAI ACGGC 1 cut(s) 216
BciT130I CCWGG 2 cut(s) 172, 786
BciVI GTATCC 1 cut(s) 345
BfaI CTAG 7 cut(s) 356, 492, 765, 810, 852, 1025, 1121
BfoI RGCGCY 1 cut(s) 1065
BfuI GTATCC 1 cut(s) 345
BglI GCCNNNNNGGC 1 cut(s) 538
BisI GCNGC 4 cut(s) 53, 750, 848, 856
BlsI GCNGC 4 cut(s) 54, 751, 849, 857
Bme1390I CCNGG 2 cut(s) 172, 786
Bme18I GGWCC 4 cut(s) 311, 425, 498, 782
BmeRI GACNNNNNGTC 1 cut(s) 909
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 9 cut(s) 151, 152, 236, 237, 311, 332, 425, 498, 782
BmiI GGNNCC 8 cut(s) 153, 163, 217, 238, 265, 300, 413, 426
BmrFI CCNGG 2 cut(s) 172, 786
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 3 cut(s) 194, 703, 935
BmtI GCTAGC 1 cut(s) 813
BmuI ACTGGG 1 cut(s) 486
BpiI GAAGAC 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 466
BpuEI CTTGAG 1 cut(s) 458
BsaBI GATNNNNATC 1 cut(s) 925
BsaJI CCNNGG 2 cut(s) 232, 785
Bse118I RCCGGY 1 cut(s) 936
Bse1I ACTGG 3 cut(s) 39, 481, 775
Bse8I GATNNNNATC 1 cut(s) 925
BseBI CCWGG 2 cut(s) 172, 786
BseDI CCNNGG 2 cut(s) 232, 785
BseGI GGATG 2 cut(s) 398, 718
BseJI GATNNNNATC 1 cut(s) 925
BseMII CTCAG 2 cut(s) 171, 327
BseNI ACTGG 3 cut(s) 39, 481, 775
BseRI GAGGAG 1 cut(s) 675
BseSI GKGCMC 2 cut(s) 155, 240
BseXI GCAGC 2 cut(s) 64, 834
BseYI CCCAGC 2 cut(s) 26, 528
BsgI GTGCAG 1 cut(s) 660
BshFI GGCC 6 cut(s) 5, 153, 231, 238, 334, 541
BsiHKAI GWGCWC 1 cut(s) 446
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 2 cut(s) 222, 937
BslFI GGGAC 1 cut(s) 889
BsmFI GGGAC 1 cut(s) 889
BsmI GAATGC 2 cut(s) 1041, 1137
BsnI GGCC 6 cut(s) 5, 153, 231, 238, 334, 541
BsoBI CYCGRG 1 cut(s) 290
Bsp119I TTCGAA 1 cut(s) 466
Bsp120I GGGCCC 2 cut(s) 151, 236
Bsp1286I GDGCHC 3 cut(s) 155, 240, 446
Bsp143I GATC 5 cut(s) 161, 215, 256, 411, 614
BspACI CCGC 6 cut(s) 195, 225, 579, 750, 855, 960
BspANI GGCC 6 cut(s) 5, 153, 231, 238, 334, 541
BspCNI CTCAG 2 cut(s) 172, 328
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 8 cut(s) 153, 163, 217, 238, 265, 300, 413, 426
BspOI GCTAGC 1 cut(s) 813
BspPI GGATC 6 cut(s) 156, 169, 210, 223, 406, 419
BspT104I TTCGAA 1 cut(s) 466
BsrFI RCCGGY 1 cut(s) 936
BsrI ACTGG 3 cut(s) 39, 481, 775
BssAI RCCGGY 1 cut(s) 936
BssECI CCNNGG 2 cut(s) 232, 785
BssMI GATC 5 cut(s) 161, 215, 256, 411, 614
BssSI CACGAG 1 cut(s) 657
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 2 cut(s) 172, 786
Bst6I CTCTTC 1 cut(s) 1117
BstAPI GCANNNNNTGC 1 cut(s) 521
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 1 cut(s) 811
BstDEI CTNAG 2 cut(s) 180, 336
BstDSI CCRYGG 1 cut(s) 232
BstENI CCTNNNNNAGG 1 cut(s) 657
BstF5I GGATG 2 cut(s) 398, 718
BstH2I RGCGCY 1 cut(s) 1065
BstHHI GCGC 1 cut(s) 1064
BstKTI GATC 5 cut(s) 164, 218, 259, 414, 617
BstMBI GATC 5 cut(s) 161, 215, 256, 411, 614
BstMWI GCNNNNNNNGC 4 cut(s) 58, 521, 538, 806
BstNI CCWGG 2 cut(s) 172, 786
BstSCI CCNGG 2 cut(s) 170, 784
BstSLI GKGCMC 2 cut(s) 155, 240
BstV1I GCAGC 2 cut(s) 64, 834
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 3 cut(s) 161, 215, 411
BstXI CCANNNNNNTGG 1 cut(s) 631
BstYI RGATCY 3 cut(s) 161, 215, 411
BsuI GTATCC 1 cut(s) 345
BsuRI GGCC 6 cut(s) 5, 153, 231, 238, 334, 541
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 2 cut(s) 398, 718
BtsI GCAGTG 2 cut(s) 648, 820
BtsIMutI CAGTG 3 cut(s) 177, 648, 820
Cac8I GCNNGC 1 cut(s) 811
CaiI CAGNNNCTG 1 cut(s) 61
CciI TCATGA 1 cut(s) 103
CfoI GCGC 1 cut(s) 1064
Cfr10I RCCGGY 1 cut(s) 936
Cfr13I GGNCC 9 cut(s) 151, 152, 236, 237, 311, 332, 425, 498, 782
Csp6I GTAC 3 cut(s) 8, 42, 1124
CviAII CATG 6 cut(s) 104, 575, 625, 917, 971, 1067
CviQI GTAC 3 cut(s) 8, 42, 1124
DdeI CTNAG 2 cut(s) 180, 336
DpnI GATC 5 cut(s) 163, 217, 258, 413, 616
DpnII GATC 5 cut(s) 161, 215, 256, 411, 614
DriI GACNNNNNGTC 1 cut(s) 909
EaeI YGGCCR 2 cut(s) 3, 229
Eam1104I CTCTTC 1 cut(s) 1117
Eam1105I GACNNNNNGTC 1 cut(s) 909
EarI CTCTTC 1 cut(s) 1117
Ecl136II GAGCTC 1 cut(s) 444
Eco24I GRGCYC 3 cut(s) 155, 240, 446
Eco47I GGWCC 4 cut(s) 311, 425, 498, 782
Eco53kI GAGCTC 1 cut(s) 444
Eco57I CTGAAG 2 cut(s) 83, 870
Eco88I CYCGRG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 444
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 170, 784
EcoT38I GRGCYC 3 cut(s) 155, 240, 446
FaeI CATG 6 cut(s) 107, 578, 628, 920, 974, 1070
FaqI GGGAC 1 cut(s) 889
FatI CATG 6 cut(s) 103, 574, 624, 916, 970, 1066
FauNDI CATATG 1 cut(s) 420
Fnu4HI GCNGC 4 cut(s) 53, 750, 848, 856
FokI GGATG 2 cut(s) 385, 725
FriOI GRGCYC 3 cut(s) 155, 240, 446
Fsp4HI GCNGC 4 cut(s) 53, 750, 848, 856
FspBI CTAG 7 cut(s) 356, 492, 765, 810, 852, 1025, 1121
GlaI GCGC 1 cut(s) 1063
GluI GCNGC 4 cut(s) 53, 750, 848, 856
GsaI CCCAGC 2 cut(s) 30, 532
HaeII RGCGCY 1 cut(s) 1065
HaeIII GGCC 6 cut(s) 5, 153, 231, 238, 334, 541
HapII CCGG 2 cut(s) 222, 937
HhaI GCGC 1 cut(s) 1064
Hin1II CATG 6 cut(s) 107, 578, 628, 920, 974, 1070
Hin6I GCGC 1 cut(s) 1062
HinP1I GCGC 1 cut(s) 1062
HindIII AAGCTT 1 cut(s) 1164
HinfI GANTC 6 cut(s) 134, 167, 380, 431, 568, 904
HpaII CCGG 2 cut(s) 222, 937
Hpy166II GTNNAC 3 cut(s) 988, 1084, 1160
Hpy188I TCNGA 7 cut(s) 166, 256, 268, 379, 567, 826, 889
Hpy188III TCNNGA 5 cut(s) 104, 290, 402, 428, 765
Hpy8I GTNNAC 3 cut(s) 988, 1084, 1160
HpyAV CCTTC 2 cut(s) 489, 705
HpyCH4V TGCA 6 cut(s) 641, 646, 674, 716, 728, 926
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 521, 538, 806
HpyF3I CTNAG 2 cut(s) 180, 336
Hsp92II CATG 6 cut(s) 107, 578, 628, 920, 974, 1070
HspAI GCGC 1 cut(s) 1062
Kzo9I GATC 5 cut(s) 161, 215, 256, 411, 614
Lsp1109I GCAGC 2 cut(s) 64, 834
LweI GCATC 3 cut(s) 194, 703, 935
MaeI CTAG 7 cut(s) 356, 492, 765, 810, 852, 1025, 1121
MaeIII GTNAC 1 cut(s) 344
MalI GATC 5 cut(s) 163, 217, 258, 413, 616
MboI GATC 5 cut(s) 161, 215, 256, 411, 614
MboII GAAGA 5 cut(s) 83, 86, 89, 469, 1104
MfeI CAATTG 1 cut(s) 689
MflI RGATCY 3 cut(s) 161, 215, 411
MhlI GDGCHC 3 cut(s) 155, 240, 446
MluCI AATT 6 cut(s) 83, 373, 628, 689, 743, 774
MlyI GAGTC 4 cut(s) 374, 425, 562, 898
MroXI GAANNNNTTC 1 cut(s) 373
MseI TTAA 1 cut(s) 777
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 2 cut(s) 222, 937
MspR9I CCNGG 2 cut(s) 172, 786
MunI CAATTG 1 cut(s) 689
Mva1269I GAATGC 2 cut(s) 1041, 1137
MvaI CCWGG 2 cut(s) 172, 786
MwoI GCNNNNNNNGC 4 cut(s) 58, 521, 538, 806
NdeI CATATG 1 cut(s) 420
NdeII GATC 5 cut(s) 161, 215, 256, 411, 614
NheI GCTAGC 1 cut(s) 809
NlaIII CATG 6 cut(s) 107, 578, 628, 920, 974, 1070
NlaIV GGNNCC 8 cut(s) 153, 163, 217, 238, 265, 300, 413, 426
NspV TTCGAA 1 cut(s) 466
PaeR7I CTCGAG 1 cut(s) 290
PagI TCATGA 1 cut(s) 103
PctI GAATGC 2 cut(s) 1041, 1137
PdmI GAANNNNTTC 1 cut(s) 373
PfeI GAWTC 2 cut(s) 134, 167
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 4 cut(s) 54, 751, 849, 857
PleI GAGTC 4 cut(s) 374, 425, 562, 898
PpsI GAGTC 4 cut(s) 374, 425, 562, 898
PpuMI RGGWCCY 1 cut(s) 425
Psp124BI GAGCTC 1 cut(s) 446
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 2 cut(s) 170, 784
PspFI CCCAGC 2 cut(s) 26, 528
PspGI CCWGG 2 cut(s) 170, 784
PspN4I GGNNCC 8 cut(s) 153, 163, 217, 238, 265, 300, 413, 426
PspOMI GGGCCC 2 cut(s) 151, 236
PspPI GGNCC 9 cut(s) 151, 152, 236, 237, 311, 332, 425, 498, 782
PspPPI RGGWCCY 1 cut(s) 425
PstNI CAGNNNCTG 1 cut(s) 61
PsuI RGATCY 3 cut(s) 161, 215, 411
RsaI GTAC 3 cut(s) 9, 43, 1125
RsaNI GTAC 3 cut(s) 8, 42, 1124
SacI GAGCTC 1 cut(s) 446
SaqAI TTAA 1 cut(s) 777
SatI GCNGC 4 cut(s) 53, 750, 848, 856
Sau3AI GATC 5 cut(s) 161, 215, 256, 411, 614
Sau96I GGNCC 9 cut(s) 151, 152, 236, 237, 311, 332, 425, 498, 782
SchI GAGTC 4 cut(s) 374, 425, 562, 898
ScrFI CCNGG 2 cut(s) 172, 786
SduI GDGCHC 3 cut(s) 155, 240, 446
SfaNI GCATC 3 cut(s) 194, 703, 935
Sfr274I CTCGAG 1 cut(s) 290
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 4 cut(s) 311, 425, 498, 782
SlaI CTCGAG 1 cut(s) 290
SmlI CTYRAG 2 cut(s) 290, 437
SmoI CTYRAG 2 cut(s) 290, 437
Sse9I AATT 6 cut(s) 83, 373, 628, 689, 743, 774
SsiI CCGC 6 cut(s) 195, 225, 579, 750, 855, 960
SspI AATATT 1 cut(s) 598
SspMI CTAG 7 cut(s) 356, 492, 765, 810, 852, 1025, 1121
SstI GAGCTC 1 cut(s) 446
StyD4I CCNGG 2 cut(s) 170, 784
TaqI TCGA 4 cut(s) 291, 466, 978, 1074
TasI AATT 6 cut(s) 83, 373, 628, 689, 743, 774
TatI WGTACW 1 cut(s) 1123
TauI GCSGC 2 cut(s) 752, 858
TfiI GAWTC 2 cut(s) 134, 167
Tru1I TTAA 1 cut(s) 777
Tru9I TTAA 1 cut(s) 777
TscAI CASTG 3 cut(s) 184, 648, 820
TseI GCWGC 2 cut(s) 52, 847
TspDTI ATGAA 9 cut(s) 92, 134, 387, 470, 525, 641, 674, 1042, 1138
TspRI CASTG 3 cut(s) 184, 648, 820
Van91I CCANNNNNTGG 1 cut(s) 308
VpaK11BI GGWCC 4 cut(s) 311, 425, 498, 782
XagI CCTNNNNNAGG 1 cut(s) 657
XapI RAATTY 1 cut(s) 628
XbaI TCTAGA 1 cut(s) 764
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 373
XspI CTAG 7 cut(s) 356, 492, 765, 810, 852, 1025, 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.