pycom09g04670

Pentatricopeptide repeat-containing protein At5g65560-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0015670
Physical Location & Seq
Reverse (-)
3451738 .. 3454497
2760 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g04670.1

Sequence Viewer

Length: 2760 bp
ATGTTAAAGCCCCTCCAAACCCTCCACGTCCATACCCTCCGCTCTCTATTGGGTTCCGACCATTTTCTCAAATTCTCAACAAGAACCAGGCTTTTCTCCGAACCCGCTCAACCCTCCAAACCTTTCCAGGACTTGGTCGCCAGAATCTGCGACATTTTGAGCGACCCCCAATGGGAACGGAGCTCGGAGCTCAGCTGGCTCGGACCCGAGCTCAGAACCCATCACGTGTCGAAGATCATCGAGTCCCACCGTAACACTGACTCGGCGTTGAGATTCTTCTACTGGGTTTCTAAGAGGCATTTTTACAAACACGATATGGGCTGCTTCTCGTCAATGCTTAATCGGCTTGTGAGGGAGCGGCTTTTTGCGCCTGCGGACCGTGTGAGGATTTTGATGATCAAGGCTTGTAGGAAGGAGGAAGAGCTCAAATGGGTAATTGAGTATTTGAATGATTTGAATGGACTTGGTTTTCAGTTCACATTGTTTAGTTTTAATACACTTTTGATACAATTGGGAAAGTTCGAGATGGTTAGTGTAGCTCGAAATGTTTATACTCAGATGCTTAATAGTGGGATTAGACCAAGTTTGTTGACATTTAATACAATGATCAATATATTGTGTAAGAAAGGCAGGGTTCAGGAAGCAGAGGTGATTTTGAGCCGGATTTTTCAGTTCGATATGTTCCCGGATGTTTTTACTTATACATCGTTGATTCTTGGGCATTGTAGAAATCGTAAGGTGAATTTGGCATTTGAAGTTTATGATCAGATGGTGAAGGCAGGTTGTGACCCCAATTCAGTTACGTATTCAACTCTCATCAATGGGCTATGCAATGAGGGAAGGGTGGATGAGGCACTGGATATGCTTGATGAAATGGTAGAGAAAGGGATTGAACCCACAGTATTTACTTACACTGTCCCAATTACTTCACTATGTGAGGCCAATCGACTGGTGGAAGCAATTGGGCTTTTCAGAAGTATGAGGGGCAGGGGTTGCCATCCTAACGTTCATACTTATACAGCATTGATCAGCGGGTTGTCTCAGACAGGAAAACTCAATGTTGCTGTTGGACTCTATCACAAACTTGTGAAGGACGGTTTGGTCCCAAACACGGTTACCTTCAATACATTGATAAATGAATTATGTGAGACAGGAAGATATGGCATGGCTCAGAAGATTTTTTATTGGATGGAAAGACATGGTACCATGTCAAATACCCAAACACACAACGGAATCATCAAGGTTTTCTGTTTGATGGGTAACATTAATAAGGCAATGACGCTTTTAAGCAAAATGCTGAAAGTGGGACCCTCTCCAAATGTGATAACTTATAACACACTTATCAATGCATACCTTGATGGGGGTAAATTAAACAATGCAGTGAGGTTATTGGATTTGATGAAATGGAGTGGATGTGAACCAGATGAATGGACTTATACTGAACTTATTTCTGGGTTATGCAAGGAGGGTAAGTCTGAAGATGCATCTACTATTTTCCATGAGATGGTAGAACAAGGCATTCTTCCCAATCAGGTCACTTACACTGCTTTGATTGCTGGATATTGTGCGGAGGGGAAGGTAGATGCTGCATTGGCATTATTTGAGCGGATGGAGGAGGATGGTTGCTGTCCGGGTATTGAAACCTACAATACCATTATAAGTGGTTTGTGCAATGATAATCAGATTGAAAAAGCTAAAAAATTATGTACAAAGATGGGGGAGCAAGGACTGGCTCCAAATGTCATTACCTACACGTCTCTAATTAGGTGCCTCTGTGGGAATGGCAGTACTGACGTTGCATTCCAAATCTACCATGAAATGGGAAAGCAGGGTTGCTTGCCGAACTTGTATACATATAGTTCACTCATTTTTGGGTTATGTCGTGAGGGTAAGGCTGATGATGCTGAGAGGTTACTTAAAGAAATGGAGAGGAAAGGATTGGTTCCTGATGAGGTAACATATACCACACTCATTGATGGATTTGTTATGCTTGGCAAGCTAGATCATGCGTTTCTGCTCCTTAGGCGAATGGTTGATGTTGGCTGCAAGCCCAACTACCGAACTTATTCTGTGTTGGTCAAGGGGTTGCAAAGGGAAAGCCAGTTGCTGACAGAGAATGTTGTGGGCCTTGAGTCCCAACATGAAGAAACGTATAGTTGCAGCTCTGATGAGAGCTATACCTTTTTTGAGGTGTTGTGTAATCTCTTAGCTAGGATGTCAGAGTATGGATGTGAACCAACTGTTCATACCTATGATATTTTAGTGAGAGGCTTGTGCAGAGATGGCAGATATTATGTGGCGGATCAGTTGCTGCAGCATATGAAAGATAAAGGGTTCTGCCCCACTAATAAGATTTATCTGTCTCTATTCTTTGTTTATTGTACAAATTTGAAAGTGGATTCTGCGCTGGAAATTTTTGGTTTGATGGCAGATAGAGGATTTGAGGTTCACTTATCATCTTATAAGGCACTTATTAGTGTCCTCTGCAGGGTGGGTCGAGTGGAAGAAGCTGAAACATTATTCAGAAGGATGCTAGAGAAACAATGGAATACTGATGAGATTGTTTGGACTGTGTTAATTGATGGCTTACTGAAGGAAGGGAATTCAGATTTATGCATGAAGTTTCTTCATGTTATTGAATCTGAAAAATGCAGTATCAGTACCGAGACGTATGTTATTTTGGCCAGAGAACTTTCTAAAATAAACGATACTGTGGTGACTTCGCAAATTGTTAACAAAGATTTGAAGGATGTACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

920

Amino Acids

104.39

Weight (kDa)

6.26

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 162 - 208 3e-07 PPR repeat family
PPR_1 PF12854 191 - 220 6.4e-08 PPR repeat
PPR_2 PF13041 194 - 243 1.3e-10 PPR repeat family
PPR_3 PF13812 227 - 274 2.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 227 - 258 1.3e-09 PPR repeat
PPR_2 PF13041 229 - 278 1.3e-20 PPR repeat family
PPR PF01535 232 - 262 1.2e-06 PPR repeat
TPR_24 PF23276 243 - 361 8e-09 Fungal tetratrico peptide repeats
PPR_long PF17177 248 - 350 1.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 261 - 293 2.7e-16 PPR repeat
PPR PF01535 267 - 297 2.1e-10 PPR repeat
PPR_2 PF13041 271 - 312 2e-14 PPR repeat family
PPR_1 PF12854 295 - 328 3.8e-07 PPR repeat
PPR_2 PF13041 308 - 347 1.8e-10 PPR repeat family
PPR_3 PF13812 323 - 379 4.6e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 330 - 360 3.3e-06 PPR repeat
PPR_2 PF13041 338 - 382 1.4e-11 PPR repeat family
PPR_long PF17177 347 - 491 2.9e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 365 - 394 7.6e-07 PPR repeat
PPR_2 PF13041 369 - 417 8.6e-12 PPR repeat family
PPR_3 PF13812 393 - 451 3.6e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 410 - 451 1.5e-08 PPR repeat family
PPR_1 PF12854 436 - 468 3.3e-08 PPR repeat
PPR_2 PF13041 439 - 488 4.2e-17 PPR repeat family
PPR PF01535 442 - 472 8.1e-06 PPR repeat
PPR_3 PF13812 462 - 514 1.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 471 - 503 1.9e-10 PPR repeat
PPR PF01535 478 - 507 8.5e-07 PPR repeat
PPR_2 PF13041 478 - 522 4.4e-14 PPR repeat family
PPR_long PF17177 493 - 579 7e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 498 - 554 5.6e-15 Pentatricopeptide repeat domain
PPR_1 PF12854 506 - 538 9.2e-12 PPR repeat
PPR_2 PF13041 509 - 558 4.7e-17 PPR repeat family
PPR PF01535 512 - 542 2.5e-09 PPR repeat
PPR_1 PF12854 540 - 572 5.5e-09 PPR repeat
PPR_2 PF13041 544 - 592 8.1e-16 PPR repeat family
PPR_3 PF13812 568 - 623 1e-11 Pentatricopeptide repeat domain
PPR_long PF17177 570 - 691 3e-12 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 576 - 607 8.2e-09 PPR repeat
PPR_2 PF13041 579 - 628 3.7e-17 PPR repeat family
PPR_1 PF12854 611 - 643 8.2e-14 PPR repeat
PPR PF01535 617 - 647 2.6e-08 PPR repeat
PPR_2 PF13041 622 - 662 4.6e-13 PPR repeat family
PPR_3 PF13812 637 - 694 2.1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 645 - 678 1.9e-09 PPR repeat
PPR_2 PF13041 652 - 696 1.1e-11 PPR repeat family
PPR PF01535 652 - 682 3.4e-06 PPR repeat
PPR_1 PF12854 743 - 775 6e-07 PPR repeat
PPR_1 PF12854 813 - 844 1.9e-06 PPR repeat
PPR PF01535 820 - 847 1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015670)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g41080 FvH4_6g41080 FvH4_6g41080
malus_domestica MD09G1122300.v1.1
prunus_persica Prupe.3G201200_v2.0.a1
pyrus_communis pycom09g04670
rosa_chinensis RchiOBHm_Chr2g0156641
rosa_laevigata RLG00000020927
rosa_multiflora Rmu_sc0018228.1_g000003
rosa_roxburghii Rroxscaffold_2G00092880
rosa_rugosa Rorug02G0454900
rosa_samantha Rh2AG520600 Rh2BG533700 Rh2CG505600 Rh2DG542500
rosa_wichuraiana Rw2G042950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1332, 1658, 2463
AasI GACNNNNNNGTC 1 cut(s) 1100
Acc36I ACCTGC 1 cut(s) 770
Acc65I GGTACC 1 cut(s) 1202
AccB1I GGYRCC 2 cut(s) 1202, 1767
AccB7I CCANNNNNTGG 3 cut(s) 133, 1504, 1819
AccBSI CCGCTC 4 cut(s) 42, 107, 358, 1606
AccI GTMKAC 1 cut(s) 1850
AciI CCGC 8 cut(s) 40, 105, 358, 374, 1032, 1568, 1606, 2300
AclI AACGTT 1 cut(s) 1005
AclWI GGATC 1 cut(s) 2310
AcoI YGGCCR 1 cut(s) 2682
AcsI RAATTY 5 cut(s) 71, 742, 2386, 2412, 2602
AcuI CTGAAG 2 cut(s) 1497, 2612
AcvI CACGTG 1 cut(s) 226
AdeI CACNNNGTG 1 cut(s) 935
AfaI GTAC 6 cut(s) 1204, 1708, 1789, 2383, 2662, 2754
AfiI CCNNNNNNNGG 7 cut(s) 133, 172, 1111, 1360, 1504, 1819, 2488
AflIII ACRYGT 2 cut(s) 225, 1752
AjiI CACGTC 2 cut(s) 28, 1755
AjnI CCWGG 2 cut(s) 86, 126
AjuI GAANNNNNNNTTGG 2 cut(s) 1082, 1114
AloI GAACNNNNNNTCC 2 cut(s) 2430, 2462
Alw21I GWGCWC 4 cut(s) 185, 192, 213, 426
Alw26I GTCTC 5 cut(s) 1044, 1142, 1761, 2367, 2660
AlwI GGATC 1 cut(s) 2310
AlwNI CAGNNNCTG 3 cut(s) 147, 2107, 2311
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 3 cut(s) 939, 2125, 2682
ApeKI GCWGC 6 cut(s) 321, 1586, 2043, 2160, 2311, 2314
ApoI RAATTY 5 cut(s) 71, 742, 2386, 2412, 2602
AseI ATTAAT 1 cut(s) 1266
Asp700I GAANNNNTTC 1 cut(s) 2065
Asp718I GGTACC 1 cut(s) 1202
AspLEI GCGC 2 cut(s) 370, 2407
AspS9I GGNCC 5 cut(s) 203, 376, 1102, 1307, 2125
AsuC2I CCSGG 2 cut(s) 686, 1632
AsuHPI GGTGA 4 cut(s) 661, 751, 784, 2728
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 4 cut(s) 203, 376, 1102, 1307
AxyI CCTNAGG 1 cut(s) 2021
BaeI ACNNNNGTAYC 2 cut(s) 2700, 2733
BalI TGGCCA 1 cut(s) 2684
BanI GGYRCC 2 cut(s) 1202, 1767
BanII GRGCYC 4 cut(s) 185, 192, 213, 426
BbrPI CACGTG 1 cut(s) 226
Bbv12I GWGCWC 4 cut(s) 185, 192, 213, 426
BbvI GCAGC 6 cut(s) 308, 1573, 2030, 2172, 2298, 2326
BciT130I CCWGG 2 cut(s) 88, 128
BclI TGATCA 4 cut(s) 396, 606, 763, 1026
BcnI CCSGG 2 cut(s) 686, 1632
BcoDI GTCTC 5 cut(s) 1044, 1142, 1761, 2367, 2660
BfaI CTAG 3 cut(s) 2000, 2211, 2534
BfmI CTRYAG 2 cut(s) 2312, 2485
BfuAI ACCTGC 1 cut(s) 770
BisI GCNGC 7 cut(s) 322, 359, 1587, 2044, 2161, 2312, 2315
BlpI GCTNAGC 1 cut(s) 191
BlsI GCNGC 7 cut(s) 323, 360, 1588, 2045, 2162, 2313, 2316
BmcAI AGTACT 1 cut(s) 1789
Bme1390I CCNGG 4 cut(s) 88, 128, 686, 1632
Bme18I GGWCC 4 cut(s) 203, 376, 1102, 1307
BmeT110I CYCGRG 1 cut(s) 206
BmgBI CACGTC 2 cut(s) 28, 1755
BmgT120I GGNCC 5 cut(s) 203, 376, 1102, 1307, 2125
BmiI GGNNCC 9 cut(s) 55, 205, 1104, 1204, 1308, 1309, 1734, 1769, 1944
BmrFI CCNGG 4 cut(s) 88, 128, 686, 1632
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 6 cut(s) 549, 1471, 1493, 1573, 1891, 2520
BmuI ACTGGG 1 cut(s) 292
Bpu1102I GCTNAGC 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 2150
BpuMI CCSGG 2 cut(s) 686, 1632
BsaAI YACGTR 2 cut(s) 226, 804
BsaXI ACNNNNNCTCC 4 cut(s) 1399, 1429, 1457, 1487
Bsc4I CCNNNNNNNGG 7 cut(s) 133, 172, 1111, 1360, 1504, 1819, 2488
Bse1I ACTGG 5 cut(s) 287, 861, 954, 1734, 2101
Bse21I CCTNAGG 1 cut(s) 2021
Bse3DI GCAATG 3 cut(s) 838, 1281, 1678
BseBI CCWGG 2 cut(s) 88, 128
BseLI CCNNNNNNNGG 7 cut(s) 133, 172, 1111, 1360, 1504, 1819, 2488
BseMI GCAATG 3 cut(s) 838, 1281, 1678
BseMII CTCAG 6 cut(s) 205, 226, 569, 1055, 1184, 1896
BseNI ACTGG 5 cut(s) 287, 861, 954, 1734, 2101
BseRI GAGGAG 1 cut(s) 1628
BseXI GCAGC 6 cut(s) 308, 1573, 2030, 2172, 2298, 2326
BsgI GTGCAG 1 cut(s) 2296
BshFI GGCC 3 cut(s) 941, 2127, 2684
BshNI GGYRCC 2 cut(s) 1202, 1767
BsiHKAI GWGCWC 4 cut(s) 185, 192, 213, 426
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 3 cut(s) 661, 686, 1631
BslFI GGGAC 5 cut(s) 229, 902, 1088, 1320, 2119
BslI CCNNNNNNNGG 7 cut(s) 133, 172, 1111, 1360, 1504, 1819, 2488
BsmAI GTCTC 5 cut(s) 1044, 1142, 1761, 2367, 2660
BsmBI CGTCTC 2 cut(s) 1761, 2660
BsmFI GGGAC 5 cut(s) 229, 902, 1088, 1320, 2119
BsmI GAATGC 2 cut(s) 1518, 1799
BsnI GGCC 3 cut(s) 941, 2127, 2684
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 4 cut(s) 185, 192, 213, 426
Bsp1407I TGTACA 3 cut(s) 1706, 2381, 2752
Bsp143I GATC 7 cut(s) 234, 396, 606, 763, 1026, 2002, 2302
Bsp1720I GCTNAGC 1 cut(s) 191
BspACI CCGC 8 cut(s) 40, 105, 358, 374, 1032, 1568, 1606, 2300
BspANI GGCC 3 cut(s) 941, 2127, 2684
BspCNI CTCAG 6 cut(s) 204, 225, 568, 1054, 1183, 1897
BspLI GGNNCC 9 cut(s) 55, 205, 1104, 1204, 1308, 1309, 1734, 1769, 1944
BspMAI CTGCAG 2 cut(s) 2316, 2489
BspMI ACCTGC 1 cut(s) 770
BspPI GGATC 1 cut(s) 2310
BspQI GCTCTTC 1 cut(s) 414
BspT107I GGYRCC 2 cut(s) 1202, 1767
BsrBI CCGCTC 4 cut(s) 42, 107, 358, 1606
BsrDI GCAATG 3 cut(s) 838, 1281, 1678
BsrGI TGTACA 3 cut(s) 1706, 2381, 2752
BsrI ACTGG 5 cut(s) 287, 861, 954, 1734, 2101
BssMI GATC 7 cut(s) 234, 396, 606, 763, 1026, 2002, 2302
BssNAI GTATAC 1 cut(s) 1851
Bst1107I GTATAC 1 cut(s) 1851
Bst2UI CCWGG 2 cut(s) 88, 128
Bst4CI ACNGT 9 cut(s) 251, 380, 901, 916, 1097, 1114, 2242, 2572, 2713
Bst6I CTCTTC 1 cut(s) 414
BstAPI GCANNNNNTGC 1 cut(s) 993
BstAUI TGTACA 3 cut(s) 1706, 2381, 2752
BstBAI YACGTR 2 cut(s) 226, 804
BstC8I GCNNGC 5 cut(s) 197, 372, 1838, 1997, 2048
BstDEI CTNAG 9 cut(s) 191, 212, 291, 555, 1041, 1170, 1905, 2021, 2206
BstEII GGTNACC 1 cut(s) 1114
BstHHI GCGC 2 cut(s) 370, 2407
BstKTI GATC 7 cut(s) 237, 399, 609, 766, 1029, 2005, 2305
BstMAI GTCTC 5 cut(s) 1044, 1142, 1761, 2367, 2660
BstMBI GATC 7 cut(s) 234, 396, 606, 763, 1026, 2002, 2302
BstNI CCWGG 2 cut(s) 88, 128
BstPI GGTNACC 1 cut(s) 1114
BstSCI CCNGG 4 cut(s) 86, 126, 684, 1630
BstSFI CTRYAG 2 cut(s) 2312, 2485
BstSNI TACGTA 1 cut(s) 804
BstV1I GCAGC 6 cut(s) 308, 1573, 2030, 2172, 2298, 2326
BstXI CCANNNNNNTGG 2 cut(s) 949, 1428
BstZ17I GTATAC 1 cut(s) 1851
Bsu36I CCTNAGG 1 cut(s) 2021
BsuRI GGCC 3 cut(s) 941, 2127, 2684
BtrI CACGTC 2 cut(s) 28, 1755
BtsI GCAGTG 2 cut(s) 1386, 1542
BtsIMutI CAGTG 5 cut(s) 255, 854, 912, 1386, 1542
BveI ACCTGC 1 cut(s) 770
Cac8I GCNNGC 5 cut(s) 197, 372, 1838, 1997, 2048
CaiI CAGNNNCTG 3 cut(s) 147, 2107, 2311
CfoI GCGC 2 cut(s) 370, 2407
Cfr13I GGNCC 5 cut(s) 203, 376, 1102, 1307, 2125
CpoI CGGWCCG 1 cut(s) 376
CseI GACGC 1 cut(s) 1288
Csp6I GTAC 6 cut(s) 1203, 1707, 1788, 2382, 2661, 2753
CspCI CAANNNNNGTGG 2 cut(s) 1954, 1989
CspI CGGWCCG 1 cut(s) 376
CviAII CATG 9 cut(s) 1165, 1199, 1207, 1499, 1814, 2006, 2141, 2617, 2630
CviQI GTAC 6 cut(s) 1203, 1707, 1788, 2382, 2661, 2753
DdeI CTNAG 9 cut(s) 191, 212, 291, 555, 1041, 1170, 1905, 2021, 2206
DpnI GATC 7 cut(s) 236, 398, 608, 765, 1028, 2004, 2304
DpnII GATC 7 cut(s) 234, 396, 606, 763, 1026, 2002, 2302
DraIII CACNNNGTG 1 cut(s) 935
DrdI GACNNNNNNGTC 1 cut(s) 1100
DseDI GACNNNNNNGTC 1 cut(s) 1100
EaeI YGGCCR 1 cut(s) 2682
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
EciI GGCGGA 1 cut(s) 2315
Ecl136II GAGCTC 4 cut(s) 183, 190, 211, 424
Eco105I TACGTA 1 cut(s) 804
Eco24I GRGCYC 4 cut(s) 185, 192, 213, 426
Eco47I GGWCC 4 cut(s) 203, 376, 1102, 1307
Eco53kI GAGCTC 4 cut(s) 183, 190, 211, 424
Eco57I CTGAAG 2 cut(s) 1497, 2612
Eco72I CACGTG 1 cut(s) 226
Eco81I CCTNAGG 1 cut(s) 2021
Eco88I CYCGRG 1 cut(s) 206
Eco91I GGTNACC 1 cut(s) 1114
EcoICRI GAGCTC 4 cut(s) 183, 190, 211, 424
EcoO109I RGGNCCY 1 cut(s) 1307
EcoO65I GGTNACC 1 cut(s) 1114
EcoRI GAATTC 1 cut(s) 2602
EcoRII CCWGG 2 cut(s) 86, 126
EcoT22I ATGCAT 3 cut(s) 1351, 1486, 2618
EcoT38I GRGCYC 4 cut(s) 185, 192, 213, 426
Esp3I CGTCTC 2 cut(s) 1761, 2660
FaeI CATG 9 cut(s) 1168, 1202, 1210, 1502, 1817, 2009, 2144, 2620, 2633
FalI AAGNNNNNCTT 2 cut(s) 1506, 1538
FaqI GGGAC 5 cut(s) 229, 902, 1088, 1320, 2119
FatI CATG 9 cut(s) 1164, 1198, 1206, 1498, 1813, 2005, 2140, 2616, 2629
FauI CCCGC 2 cut(s) 112, 1025
FauNDI CATATG 1 cut(s) 2319
FbaI TGATCA 4 cut(s) 396, 606, 763, 1026
FblI GTMKAC 1 cut(s) 1850
Fnu4HI GCNGC 7 cut(s) 322, 359, 1587, 2044, 2161, 2312, 2315
FriOI GRGCYC 4 cut(s) 185, 192, 213, 426
Fsp4HI GCNGC 7 cut(s) 322, 359, 1587, 2044, 2161, 2312, 2315
FspBI CTAG 3 cut(s) 2000, 2211, 2534
GlaI GCGC 2 cut(s) 369, 2406
GluI GCNGC 7 cut(s) 322, 359, 1587, 2044, 2161, 2312, 2315
HaeIII GGCC 3 cut(s) 941, 2127, 2684
HapII CCGG 3 cut(s) 661, 686, 1631
HgaI GACGC 1 cut(s) 1288
HhaI GCGC 2 cut(s) 370, 2407
Hin1II CATG 9 cut(s) 1168, 1202, 1210, 1502, 1817, 2009, 2144, 2620, 2633
Hin6I GCGC 2 cut(s) 368, 2405
HinP1I GCGC 2 cut(s) 368, 2405
HincII GTYRAC 2 cut(s) 591, 2734
HindII GTYRAC 2 cut(s) 591, 2734
HpaI GTTAAC 1 cut(s) 2734
HpaII CCGG 3 cut(s) 661, 686, 1631
HphI GGTGA 4 cut(s) 661, 751, 784, 2728
Hpy166II GTNNAC 8 cut(s) 477, 591, 1418, 1851, 1862, 2234, 2449, 2734
Hpy188III TCNNGA 4 cut(s) 523, 638, 1883, 1946
Hpy8I GTNNAC 8 cut(s) 477, 591, 1418, 1851, 1862, 2234, 2449, 2734
HpyCH4III ACNGT 9 cut(s) 251, 380, 901, 916, 1097, 1114, 2242, 2572, 2713
HpyCH4IV ACGT 8 cut(s) 27, 225, 803, 1005, 1754, 1794, 2150, 2669
HpyF3I CTNAG 9 cut(s) 191, 212, 291, 555, 1041, 1170, 1905, 2021, 2206
HpySE526I ACGT 8 cut(s) 27, 225, 803, 1005, 1754, 1794, 2150, 2669
Hsp92II CATG 9 cut(s) 1168, 1202, 1210, 1502, 1817, 2009, 2144, 2620, 2633
HspAI GCGC 2 cut(s) 368, 2405
KflI GGGWCCC 1 cut(s) 1307
KpnI GGTACC 1 cut(s) 1206
Ksp22I TGATCA 4 cut(s) 396, 606, 763, 1026
KspAI GTTAAC 1 cut(s) 2734
Kzo9I GATC 7 cut(s) 234, 396, 606, 763, 1026, 2002, 2302
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 6 cut(s) 180, 187, 355, 1720, 1738, 2022
Lsp1109I GCAGC 6 cut(s) 308, 1573, 2030, 2172, 2298, 2326
LweI GCATC 6 cut(s) 549, 1471, 1493, 1573, 1891, 2520
MaeI CTAG 3 cut(s) 2000, 2211, 2534
MaeII ACGT 8 cut(s) 27, 225, 803, 1005, 1754, 1794, 2150, 2669
MaeIII GTNAC 9 cut(s) 251, 785, 799, 1114, 1259, 1534, 1911, 1954, 2716
MalI GATC 7 cut(s) 236, 398, 608, 765, 1028, 2004, 2304
MbiI CCGCTC 4 cut(s) 42, 107, 358, 1606
MboI GATC 7 cut(s) 234, 396, 606, 763, 1026, 2002, 2302
MfeI CAATTG 2 cut(s) 509, 960
MhlI GDGCHC 4 cut(s) 185, 192, 213, 426
MlsI TGGCCA 1 cut(s) 2684
MluNI TGGCCA 1 cut(s) 2684
MlyI GAGTC 4 cut(s) 251, 254, 1065, 2141
MmeI TCCRAC 2 cut(s) 81, 1048
Mox20I TGGCCA 1 cut(s) 2684
Mph1103I ATGCAT 3 cut(s) 1351, 1486, 2618
MroXI GAANNNNTTC 1 cut(s) 2065
MscI TGGCCA 1 cut(s) 2684
MslI CAYNNNNRTG 1 cut(s) 2250
Msp20I TGGCCA 1 cut(s) 2684
MspA1I CMGCKG 2 cut(s) 195, 1032
MspI CCGG 3 cut(s) 661, 686, 1631
MspR9I CCNGG 4 cut(s) 88, 128, 686, 1632
MunI CAATTG 2 cut(s) 509, 960
Mva1269I GAATGC 2 cut(s) 1518, 1799
MvaI CCWGG 2 cut(s) 88, 128
NciI CCSGG 2 cut(s) 686, 1632
NdeI CATATG 1 cut(s) 2319
NdeII GATC 7 cut(s) 234, 396, 606, 763, 1026, 2002, 2302
NlaIII CATG 9 cut(s) 1168, 1202, 1210, 1502, 1817, 2009, 2144, 2620, 2633
NlaIV GGNNCC 9 cut(s) 55, 205, 1104, 1204, 1308, 1309, 1734, 1769, 1944
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 3 cut(s) 785, 1534, 2716
NsiI ATGCAT 3 cut(s) 1351, 1486, 2618
PciSI GCTCTTC 1 cut(s) 414
PctI GAATGC 2 cut(s) 1518, 1799
PdmI GAANNNNTTC 1 cut(s) 2065
PfeI GAWTC 6 cut(s) 144, 273, 712, 1233, 2399, 2639
PflFI GACNNNGTC 1 cut(s) 134
PflMI CCANNNNNTGG 3 cut(s) 133, 1504, 1819
PfoI TCCNGGA 2 cut(s) 126, 684
PkrI GCNGC 7 cut(s) 323, 360, 1588, 2045, 2162, 2313, 2316
PleI GAGTC 4 cut(s) 250, 254, 1065, 2140
PmaCI CACGTG 1 cut(s) 226
PmlI CACGTG 1 cut(s) 226
PpsI GAGTC 4 cut(s) 250, 254, 1065, 2140
Ppu21I YACGTR 2 cut(s) 226, 804
PpuMI RGGWCCY 1 cut(s) 1307
PshBI ATTAAT 1 cut(s) 1266
PsiI TTATAA 3 cut(s) 1332, 1658, 2463
Psp124BI GAGCTC 4 cut(s) 185, 192, 213, 426
Psp1406I AACGTT 1 cut(s) 1005
Psp5II RGGWCCY 1 cut(s) 1307
Psp6I CCWGG 2 cut(s) 86, 126
PspCI CACGTG 1 cut(s) 226
PspEI GGTNACC 1 cut(s) 1114
PspGI CCWGG 2 cut(s) 86, 126
PspN4I GGNNCC 9 cut(s) 55, 205, 1104, 1204, 1308, 1309, 1734, 1769, 1944
PspPI GGNCC 5 cut(s) 203, 376, 1102, 1307, 2125
PspPPI RGGWCCY 1 cut(s) 1307
PstI CTGCAG 2 cut(s) 2316, 2489
PstNI CAGNNNCTG 3 cut(s) 147, 2107, 2311
PsyI GACNNNGTC 1 cut(s) 134
PvuII CAGCTG 1 cut(s) 195
RsaI GTAC 6 cut(s) 1204, 1708, 1789, 2383, 2662, 2754
RsaNI GTAC 6 cut(s) 1203, 1707, 1788, 2382, 2661, 2753
RseI CAYNNNNRTG 1 cut(s) 2250
Rsr2I CGGWCCG 1 cut(s) 376
RsrII CGGWCCG 1 cut(s) 376
SacI GAGCTC 4 cut(s) 185, 192, 213, 426
SapI GCTCTTC 1 cut(s) 414
SatI GCNGC 7 cut(s) 322, 359, 1587, 2044, 2161, 2312, 2315
Sau3AI GATC 7 cut(s) 234, 396, 606, 763, 1026, 2002, 2302
Sau96I GGNCC 5 cut(s) 203, 376, 1102, 1307, 2125
ScaI AGTACT 1 cut(s) 1789
SchI GAGTC 4 cut(s) 251, 254, 1065, 2141
ScrFI CCNGG 4 cut(s) 88, 128, 686, 1632
SduI GDGCHC 4 cut(s) 185, 192, 213, 426
SfaNI GCATC 6 cut(s) 549, 1471, 1493, 1573, 1891, 2520
SfcI CTRYAG 2 cut(s) 2312, 2485
SinI GGWCC 4 cut(s) 203, 376, 1102, 1307
SmiMI CAYNNNNRTG 1 cut(s) 2250
SmlI CTYRAG 1 cut(s) 2129
SmoI CTYRAG 1 cut(s) 2129
SnaBI TACGTA 1 cut(s) 804
SsiI CCGC 8 cut(s) 40, 105, 358, 374, 1032, 1568, 1606, 2300
SspMI CTAG 3 cut(s) 2000, 2211, 2534
SstI GAGCTC 4 cut(s) 185, 192, 213, 426
StyD4I CCNGG 4 cut(s) 86, 126, 684, 1630
TaaI ACNGT 9 cut(s) 251, 380, 901, 916, 1097, 1114, 2242, 2572, 2713
TaiI ACGT 8 cut(s) 30, 228, 806, 1008, 1757, 1797, 2153, 2672
TaqI TCGA 7 cut(s) 230, 240, 522, 541, 675, 946, 2497
TatI WGTACW 4 cut(s) 1706, 1787, 2381, 2752
TauI GCSGC 1 cut(s) 361
TfiI GAWTC 6 cut(s) 144, 273, 712, 1233, 2399, 2639
TscAI CASTG 5 cut(s) 262, 861, 919, 1386, 1549
TseFI GTSAC 3 cut(s) 785, 1534, 2716
TseI GCWGC 6 cut(s) 321, 1586, 2043, 2160, 2311, 2314
Tsp45I GTSAC 3 cut(s) 785, 1534, 2716
TspGWI ACGGA 2 cut(s) 193, 1245
TspRI CASTG 5 cut(s) 262, 861, 919, 1386, 1549
Tth111I GACNNNGTC 1 cut(s) 134
Van91I CCANNNNNTGG 3 cut(s) 133, 1504, 1819
VpaK11BI GGWCC 4 cut(s) 203, 376, 1102, 1307
VspI ATTAAT 1 cut(s) 1266
XapI RAATTY 5 cut(s) 71, 742, 2386, 2412, 2602
XcmI CCANNNNNNNNNTGG 1 cut(s) 949
XmiI GTMKAC 1 cut(s) 1850
XmnI GAANNNNTTC 1 cut(s) 2065
XspI CTAG 3 cut(s) 2000, 2211, 2534
ZrmI AGTACT 1 cut(s) 1789
Zsp2I ATGCAT 3 cut(s) 1351, 1486, 2618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.