Rh2AG520600

Pentatricopeptide repeat-containing protein At5g65560-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015670
Physical Location & Seq
Forward (+)
74786566 .. 74789334
2769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG520600.1

Sequence Viewer

Length: 2769 bp
ATGTTAAACCCCATCAAAACCCCAACTCTCCTTCACCTCAGAACCCTCCAATCTCTATTGGGTCCTCACCATTTTCCCAAACTCTCATCAAGAACCAAGCTTTTCACCCATCCCTGTCACTCCTTCCCAAACTTGGTCTCCAAAATCTGTGACATTTTGAGTGACTCCAAATGGGAAAAAAGCTCAGAGCTTTTGGGTCTGATCCACAATCTCAGAACCTATCACATGTTGAAGATTATTCAGACTCACAGGAACACAGACTCGGCTTTGAGATTCTTCTTCTGGGTTTCGAGGAGAAAACCTTACAAGCATGACATGAGCTGCTTTGTTTCAATGCTGGATAGGCTTGTGAGGGAGAAGCTTTTCGGGCCGGCAGACCATGTAAGGCTTTTGCTTGTTAAAGCTTGTAGGAATGAGGAAGAGCTTAAATGGGTGATTGGGTATTTGAATGAAAAGAAAAGAACTGGTTTTGGGTTTACATTGTATAGTTTCAATACGCTTTTGATTCAGTTGGGCAAGTTTGAGATGGTTAGCGAAGCTCAAAATGTGTATAATGAGATACTAAGTAGTGAGGTTAAGCCGAGTTTGTTGACATTTAATACTATGATCAATATATTGTGCAAGAAGGGCAAGGTTCAGGAAGCAGAGTTGATTTTGAGCAGGATTATGCAGTATGAAATGTTGCCGGATGTTTTTACTTATACATCGTTGGTTCTTGGGCATTGTAGAAGTGGTAACTTAAATTTGGCATTTGAGGTATATGACCGGATGGTGAAGGCGGGTTGTGACCCGAATTCAGTTACGTACTCAACACTTATTAATGGGTTATGCAATGAGGGGAGGGTGGATGAGGCACTGGATATGCTTGATGAAATGATAGGGAAGGGGATTGAACCCACAGTTTATACTTACACTGTCCCAATTACTTCACTTTGTGAGGCAAATCGACCATTGGAAGCAATCAGGCTTTTCGGAAGTATGACGAAGAGGGGTTGCTGTCCGAATATTCATACTTATACAGCATTGATCAGTGGGTTGTCTCGGACAGGGAAACTCGAAGTTGCTATTGGACTCTATCACAAACTGTTGAAGGATGGTATGGTTCCAAGCATGGTTACCTTCAACACAATTATGAATGAATCAATTGAGGCAGGAAGATATGACATGGGTCTGAAGATTATTTATTGGATGGAGAGACATGGTTATGGTATCTCGGAAAATACCCGAACACACAATCATATCATCAAGGGCTTATGCTTGATGGGTAAGATTCACAATGCGATGGCTCTTTTAAGCAAAATGCTTAAAGTGGGACCGTCTCCAAATGTGATTACATATAACACATTGATCAATGGATACCTCAATAGAGGTAATCTAAACAATGCTCTGAGGTTATTAGAGTTGATGAAAGGGAGAGGATGTAAACTAGATGAGTGGACGTATACCGAAGTTATTTCTGGGTGTTGCAAGGCGCACAAGTCTGAATTTGCATCTAATCTTTTCCATGATATGTTAAAACAGGGCATTAGCCCAAATCAGGTCACTTACAACGTTTTGATTGTTGGATGCTGTAATGAGGGGAAGGTAGATGCTGCCCTGTCATTTTTCAAGCAAATGGAAGAGAATGGTTGCTCTCCAGATATTGAAAGCTACAATGCCATTATACATCGTTTGTCCAAAGACAATCAGTTTGCTAAAGCCAAGCAACTGTGTAAAAAGATGGTGGATCAAGGACTGCTTCCAAATGTCATTACCTACACATCTTTAATTGATGGCCTCTGTAAGAATGGTAGTACTGACCTTGCGTACAGGATTTTCCATGAAATGAGAGAAAGAGATTGCTTGCCGAACTTGTACACTTATAGTTCACTTGTTTTTGGGTTATGTCAAGAAGGCAAGGCTGATGATGCTGAGAGGTTACTTGAAGAAATGGAGAGGGAAGGATTGGTTCCTGATGTGGTAACATTTACCACGCTCATTAATGGTTTTGTTATGCTTGGTAGGCTAGATCATGCATTCTTACTTCTTAAGCGAATGGTTGATGTTGGCTGCAAACCCAACTATTTTACTTTTACTGTGTTGGTCAAAGGGTTGCAAAGGGAAAGTCAGTTACTCACAGAAAAGGTTGTGGGCCTTGTCGCGCAACATGAAGCACAGTGTGGTTCCAGCTCTGACAAGAGATTCAACTTTTTTGAGATATTGTGTAATCTCCTAGCTAGGATGTCAGAGAATGGATGTGAACCAACTATTGATACCTACAGTATTTTAGTGAGAGGCTTGTGTGAAGATCGCAAGTATGATGAAGTAGATCAGTTGGTGGAGCATATGAAAGAGAAAGGCCTGTATCCCAGTGAAGAGTTTTACAGGCCTCTATTCTTTGCTCATTGCAAAAACTTAAAACTGGACTCTGCTCTGGAAATCTTTGGCTTGATGGCAGATAGAGGCTTTAAGGTTAACTTTTCAACCTATACAGCACTCATTTGTGCTCTCTGCAGGGCAAATCGGATGGAAGAAGCTGAAATGTCGTTGAAAAGTATGCTAGAATGCCAGTGGAATGCTGATGAGATTGTTTGGACTGTATTAATCGATGGGTTACTGAAAAAAGGGCAGTCAGATCCATGCATGCAGCTTCTTCATGTTATTGAATCTCAAAACTGCATTCTCTGGGTCGAGACATATGCTATCTTGGCCAGAGAACTTTCTCATGTAAGCAAAACTATGGCGACTTCTCAAATTGCTGACAGAGCGATAGATTTAGAAGTACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

922

Amino Acids

104.79

Weight (kDa)

7.28

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 132 - 274 3.1e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 162 - 208 6.9e-07 PPR repeat family
PPR_1 PF12854 192 - 219 4.4e-07 PPR repeat
PPR_2 PF13041 194 - 243 2.7e-10 PPR repeat family
PPR_3 PF13812 227 - 274 8.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 227 - 258 5.6e-09 PPR repeat
PPR_2 PF13041 229 - 278 7.6e-19 PPR repeat family
PPR PF01535 232 - 262 2.6e-06 PPR repeat
TPR_24 PF23276 243 - 361 1.3e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 261 - 292 3.7e-16 PPR repeat
PPR PF01535 267 - 297 7.5e-10 PPR repeat
PPR_2 PF13041 271 - 312 7.4e-14 PPR repeat family
PPR_3 PF13812 288 - 344 6.3e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 308 - 347 1.1e-10 PPR repeat family
PPR_3 PF13812 323 - 379 2.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 330 - 359 3.9e-06 PPR repeat
PPR_2 PF13041 339 - 379 3e-07 PPR repeat family
PPR_3 PF13812 403 - 454 1.7e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 403 - 434 1.3e-06 PPR repeat
PPR_2 PF13041 407 - 452 7.2e-11 PPR repeat family
PPR_1 PF12854 438 - 470 3e-09 PPR repeat
PPR_2 PF13041 441 - 490 2.1e-17 PPR repeat family
PPR PF01535 444 - 474 4.1e-07 PPR repeat
PPR_long PF17177 457 - 610 2.7e-13 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 464 - 524 5.4e-10 Pentatricopeptide repeat domain
TPR_24 PF23276 482 - 574 8.9e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 484 - 525 1.5e-12 PPR repeat family
PPR_3 PF13812 500 - 556 1.4e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 508 - 540 7e-11 PPR repeat
PPR_2 PF13041 511 - 552 1.2e-13 PPR repeat family
PPR PF01535 514 - 544 4.9e-09 PPR repeat
PPR_long PF17177 523 - 694 1.8e-14 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 546 - 595 6e-15 PPR repeat family
PPR_3 PF13812 570 - 624 1.1e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 578 - 610 3.5e-13 PPR repeat
PPR_2 PF13041 581 - 630 9e-18 PPR repeat family
TPR_24 PF23276 582 - 678 2.9e-06 Fungal tetratrico peptide repeats
PPR PF01535 584 - 614 1.4e-08 PPR repeat
PPR_1 PF12854 613 - 645 2.6e-12 PPR repeat
PPR PF01535 619 - 649 5.4e-07 PPR repeat
PPR_2 PF13041 625 - 664 3.2e-11 PPR repeat family
PPR_3 PF13812 639 - 696 2.6e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 648 - 680 2.1e-09 PPR repeat
PPR_2 PF13041 651 - 698 1.5e-13 PPR repeat family
PPR PF01535 654 - 684 7.9e-06 PPR repeat
PPR_long PF17177 736 - 859 7e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 791 - 832 3.3e-07 PPR repeat family
PPR_1 PF12854 814 - 846 1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015670)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g41080 FvH4_6g41080 FvH4_6g41080
malus_domestica MD09G1122300.v1.1
prunus_persica Prupe.3G201200_v2.0.a1
pyrus_communis pycom09g04670
rosa_chinensis RchiOBHm_Chr2g0156641
rosa_laevigata RLG00000020927
rosa_multiflora Rmu_sc0018228.1_g000003
rosa_roxburghii Rroxscaffold_2G00092880
rosa_rugosa Rorug02G0454900
rosa_samantha Rh2AG520600 Rh2BG533700 Rh2CG505600 Rh2DG542500
rosa_wichuraiana Rw2G042950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1442
AccII CGCG 1 cut(s) 2141
AciI CCGC 1 cut(s) 779
AclI AACGTT 1 cut(s) 1551
AclWI GGATC 3 cut(s) 196, 1734, 2609
AcoI YGGCCR 1 cut(s) 2688
AcsI RAATTY 3 cut(s) 742, 793, 1484
AcuI CTGAAG 1 cut(s) 1193
AdeI CACNNNGTG 2 cut(s) 935, 2159
AfaI GTAC 5 cut(s) 806, 1795, 1808, 1856, 2763
AfiI CCNNNNNNNGG 2 cut(s) 133, 1537
AflII CTTAAG 1 cut(s) 2027
AflIII ACRYGT 1 cut(s) 225
AjuI GAANNNNNNNTTGG 2 cut(s) 1050, 1082
Alw21I GWGCWC 1 cut(s) 2488
Alw26I GTCTC 5 cut(s) 142, 1044, 1189, 1323, 2666
AlwI GGATC 3 cut(s) 196, 1734, 2609
AoxI GGCC 6 cut(s) 368, 1774, 2131, 2338, 2366, 2688
ApeKI GCWGC 4 cut(s) 321, 1592, 2049, 2626
ApoI RAATTY 3 cut(s) 742, 793, 1484
ArsI GACNNNNNNTTYG 4 cut(s) 1673, 1705, 2089, 2121
AseI ATTAAT 3 cut(s) 819, 1980, 2582
Asp700I GAANNNNTTC 1 cut(s) 362
AspLEI GCGC 2 cut(s) 1474, 2143
AspS9I GGNCC 4 cut(s) 62, 368, 1313, 2131
AsuHPI GGTGA 5 cut(s) 26, 59, 97, 445, 784
AvaII GGWCC 2 cut(s) 62, 1313
BalI TGGCCA 1 cut(s) 2690
Bbv12I GWGCWC 1 cut(s) 2488
BbvI GCAGC 4 cut(s) 308, 1579, 2036, 2638
BcgI CGANNNNNNTGC 2 cut(s) 2660, 2694
BciVI GTATCC 2 cut(s) 1349, 2355
BclI TGATCA 3 cut(s) 606, 1026, 1347
BcoDI GTCTC 5 cut(s) 142, 1044, 1189, 1323, 2666
BfaI CTAG 5 cut(s) 1427, 2006, 2213, 2217, 2540
BfmI CTRYAG 2 cut(s) 2257, 2491
BfrI CTTAAG 1 cut(s) 2027
BfuI GTATCC 2 cut(s) 1349, 2355
BisI GCNGC 4 cut(s) 322, 1593, 2050, 2627
BlsI GCNGC 4 cut(s) 323, 1594, 2051, 2628
BmcAI AGTACT 1 cut(s) 1795
Bme18I GGWCC 2 cut(s) 62, 1313
BmgT120I GGNCC 4 cut(s) 62, 368, 1313, 2131
BmiI GGNNCC 5 cut(s) 63, 1104, 1314, 1950, 2164
BmrI ACTGGG 1 cut(s) 2343
BmsI GCATC 4 cut(s) 1499, 1556, 1579, 1897
BmuI ACTGGG 1 cut(s) 2343
BoxI GACNNNNGTC 1 cut(s) 1167
BpmI CTGGAG 1 cut(s) 1620
Bsa29I ATCGAT 1 cut(s) 2586
BsaAI YACGTR 1 cut(s) 804
BsaI GGTCTC 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 765
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 1405, 1435
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 1537
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 5 cut(s) 469, 861, 2349, 2406, 2548
Bse3DI GCAATG 2 cut(s) 838, 2383
BseCI ATCGAT 1 cut(s) 2586
BseLI CCNNNNNNNGG 2 cut(s) 133, 1537
BseMI GCAATG 2 cut(s) 838, 2383
BseMII CTCAG 5 cut(s) 52, 198, 226, 1379, 1902
BseNI ACTGG 5 cut(s) 469, 861, 2349, 2406, 2548
BseRI GAGGAG 1 cut(s) 307
BseXI GCAGC 4 cut(s) 308, 1579, 2036, 2638
Bsh1236I CGCG 1 cut(s) 2141
BshFI GGCC 6 cut(s) 370, 1776, 2133, 2340, 2368, 2690
BshVI ATCGAT 1 cut(s) 2586
BsiHKAI GWGCWC 1 cut(s) 2488
BsiSI CCGG 3 cut(s) 371, 686, 766
BslFI GGGAC 2 cut(s) 902, 1326
BslI CCNNNNNNNGG 2 cut(s) 133, 1537
BsmAI GTCTC 5 cut(s) 142, 1044, 1189, 1323, 2666
BsmBI CGTCTC 1 cut(s) 1323
BsmFI GGGAC 2 cut(s) 902, 1326
BsmI GAATGC 4 cut(s) 2015, 2549, 2560, 2658
BsnI GGCC 6 cut(s) 370, 1776, 2133, 2340, 2368, 2690
Bso31I GGTCTC 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 2488
Bsp1407I TGTACA 1 cut(s) 1854
Bsp143I GATC 9 cut(s) 201, 606, 1026, 1347, 1726, 2008, 2287, 2308, 2614
BspACI CCGC 1 cut(s) 779
BspANI GGCC 6 cut(s) 370, 1776, 2133, 2340, 2368, 2690
BspCNI CTCAG 5 cut(s) 51, 197, 225, 1380, 1903
BspDI ATCGAT 1 cut(s) 2586
BspFNI CGCG 1 cut(s) 2141
BspLI GGNNCC 5 cut(s) 63, 1104, 1314, 1950, 2164
BspMAI CTGCAG 1 cut(s) 2495
BspPI GGATC 3 cut(s) 196, 1734, 2609
BspQI GCTCTTC 1 cut(s) 414
BspTI CTTAAG 1 cut(s) 2027
BspTNI GGTCTC 1 cut(s) 142
BsrDI GCAATG 2 cut(s) 838, 2383
BsrFI RCCGGY 1 cut(s) 370
BsrGI TGTACA 1 cut(s) 1854
BsrI ACTGG 5 cut(s) 469, 861, 2349, 2406, 2548
BssAI RCCGGY 1 cut(s) 370
BssMI GATC 9 cut(s) 201, 606, 1026, 1347, 1726, 2008, 2287, 2308, 2614
BssNAI GTATAC 1 cut(s) 1443
Bst1107I GTATAC 1 cut(s) 1443
Bst4CI ACNGT 9 cut(s) 901, 916, 1086, 1317, 1710, 2077, 2157, 2261, 2578
Bst6I CTCTTC 4 cut(s) 414, 980, 1614, 2349
BstAFI CTTAAG 1 cut(s) 2027
BstAUI TGTACA 1 cut(s) 1854
BstBAI YACGTR 1 cut(s) 804
BstC8I GCNNGC 3 cut(s) 372, 1844, 2624
BstDEI CTNAG 6 cut(s) 38, 184, 212, 563, 1388, 1911
BstEII GGTNACC 1 cut(s) 1114
BstFNI CGCG 1 cut(s) 2141
BstHHI GCGC 2 cut(s) 1474, 2143
BstKTI GATC 9 cut(s) 204, 609, 1029, 1350, 1729, 2011, 2290, 2311, 2617
BstMAI GTCTC 5 cut(s) 142, 1044, 1189, 1323, 2666
BstMBI GATC 9 cut(s) 201, 606, 1026, 1347, 1726, 2008, 2287, 2308, 2614
BstMWI GCNNNNNNNGC 7 cut(s) 343, 367, 627, 1907, 2002, 2687, 2744
BstNSI RCATGY 2 cut(s) 229, 2626
BstPAI GACNNNNGTC 1 cut(s) 1167
BstPI GGTNACC 1 cut(s) 1114
BstSFI CTRYAG 2 cut(s) 2257, 2491
BstSNI TACGTA 1 cut(s) 804
BstUI CGCG 1 cut(s) 2141
BstV1I GCAGC 4 cut(s) 308, 1579, 2036, 2638
BstX2I RGATCY 1 cut(s) 2614
BstYI RGATCY 1 cut(s) 2614
BstZ17I GTATAC 1 cut(s) 1443
Bsu15I ATCGAT 1 cut(s) 2586
BsuI GTATCC 2 cut(s) 1349, 2355
BsuRI GGCC 6 cut(s) 370, 1776, 2133, 2340, 2368, 2690
BsuTUI ATCGAT 1 cut(s) 2586
BtgZI GCGATG 1 cut(s) 1295
BtsIMutI CAGTG 6 cut(s) 854, 912, 1036, 2162, 2356, 2555
Cac8I GCNNGC 3 cut(s) 372, 1844, 2624
CfoI GCGC 2 cut(s) 1474, 2143
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 4 cut(s) 62, 368, 1313, 2131
ClaI ATCGAT 1 cut(s) 2586
Csp6I GTAC 5 cut(s) 805, 1794, 1807, 1855, 2762
CviQI GTAC 5 cut(s) 805, 1794, 1807, 1855, 2762
DdeI CTNAG 6 cut(s) 38, 184, 212, 563, 1388, 1911
DpnI GATC 9 cut(s) 203, 608, 1028, 1349, 1728, 2010, 2289, 2310, 2616
DpnII GATC 9 cut(s) 201, 606, 1026, 1347, 1726, 2008, 2287, 2308, 2614
DraIII CACNNNGTG 2 cut(s) 935, 2159
EaeI YGGCCR 1 cut(s) 2688
Eam1104I CTCTTC 4 cut(s) 414, 980, 1614, 2349
EarI CTCTTC 4 cut(s) 414, 980, 1614, 2349
Eco105I TACGTA 1 cut(s) 804
Eco147I AGGCCT 2 cut(s) 2340, 2368
Eco31I GGTCTC 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 62, 1313
Eco57I CTGAAG 1 cut(s) 1193
Eco91I GGTNACC 1 cut(s) 1114
EcoO109I RGGNCCY 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 1114
EcoRI GAATTC 1 cut(s) 793
EcoT22I ATGCAT 2 cut(s) 2017, 2624
Esp3I CGTCTC 1 cut(s) 1323
FalI AAGNNNNNCTT 4 cut(s) 1722, 1754, 2441, 2473
FaqI GGGAC 2 cut(s) 902, 1326
FauI CCCGC 1 cut(s) 772
FauNDI CATATG 2 cut(s) 2325, 2677
FbaI TGATCA 3 cut(s) 606, 1026, 1347
FblI GTMKAC 1 cut(s) 1442
Fnu4HI GCNGC 4 cut(s) 322, 1593, 2050, 2627
Fsp4HI GCNGC 4 cut(s) 322, 1593, 2050, 2627
FspBI CTAG 5 cut(s) 1427, 2006, 2213, 2217, 2540
GlaI GCGC 2 cut(s) 1473, 2142
GluI GCNGC 4 cut(s) 322, 1593, 2050, 2627
GsuI CTGGAG 1 cut(s) 1620
HaeIII GGCC 6 cut(s) 370, 1776, 2133, 2340, 2368, 2690
HapII CCGG 3 cut(s) 371, 686, 766
HhaI GCGC 2 cut(s) 1474, 2143
Hin6I GCGC 2 cut(s) 1472, 2141
HinP1I GCGC 2 cut(s) 1472, 2141
HincII GTYRAC 2 cut(s) 591, 2455
HindII GTYRAC 2 cut(s) 591, 2455
HindIII AAGCTT 3 cut(s) 98, 359, 402
HpaI GTTAAC 1 cut(s) 2455
HpaII CCGG 3 cut(s) 371, 686, 766
HphI GGTGA 5 cut(s) 26, 59, 97, 445, 784
Hpy166II GTNNAC 9 cut(s) 477, 591, 1424, 1437, 1443, 1857, 1868, 2240, 2455
Hpy188III TCNNGA 7 cut(s) 90, 638, 1637, 1889, 1952, 2414, 2671
Hpy8I GTNNAC 9 cut(s) 477, 591, 1424, 1437, 1443, 1857, 1868, 2240, 2455
HpyCH4III ACNGT 9 cut(s) 901, 916, 1086, 1317, 1710, 2077, 2157, 2261, 2578
HpyCH4IV ACGT 3 cut(s) 803, 1439, 1551
HpyF10VI GCNNNNNNNGC 7 cut(s) 343, 367, 627, 1907, 2002, 2687, 2744
HpyF3I CTNAG 6 cut(s) 38, 184, 212, 563, 1388, 1911
HpySE526I ACGT 3 cut(s) 803, 1439, 1551
HspAI GCGC 2 cut(s) 1472, 2141
KroI GCCGGC 1 cut(s) 370
KroNI GCCGGC 1 cut(s) 372
Ksp22I TGATCA 3 cut(s) 606, 1026, 1347
KspAI GTTAAC 1 cut(s) 2455
Kzo9I GATC 9 cut(s) 201, 606, 1026, 1347, 1726, 2008, 2287, 2308, 2614
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 1 cut(s) 2320
Lsp1109I GCAGC 4 cut(s) 308, 1579, 2036, 2638
LweI GCATC 4 cut(s) 1499, 1556, 1579, 1897
MaeI CTAG 5 cut(s) 1427, 2006, 2213, 2217, 2540
MaeII ACGT 3 cut(s) 803, 1439, 1551
MalI GATC 9 cut(s) 203, 608, 1028, 1349, 1728, 2010, 2289, 2310, 2616
MboI GATC 9 cut(s) 201, 606, 1026, 1347, 1726, 2008, 2287, 2308, 2614
MfeI CAATTG 1 cut(s) 1143
MflI RGATCY 1 cut(s) 2614
MhlI GDGCHC 1 cut(s) 2488
MlsI TGGCCA 1 cut(s) 2690
MluCI AATT 8 cut(s) 742, 793, 921, 1128, 1143, 1484, 1767, 2733
MluNI TGGCCA 1 cut(s) 2690
MlyI GAGTC 5 cut(s) 158, 238, 254, 1065, 2399
MmeI TCCRAC 1 cut(s) 1543
Mox20I TGGCCA 1 cut(s) 2690
Mph1103I ATGCAT 2 cut(s) 2017, 2624
MroNI GCCGGC 1 cut(s) 370
MroXI GAANNNNTTC 1 cut(s) 362
MscI TGGCCA 1 cut(s) 2690
MslI CAYNNNNRTG 2 cut(s) 1130, 1203
Msp20I TGGCCA 1 cut(s) 2690
MspCI CTTAAG 1 cut(s) 2027
MspI CCGG 3 cut(s) 371, 686, 766
MunI CAATTG 1 cut(s) 1143
Mva1269I GAATGC 4 cut(s) 2015, 2549, 2560, 2658
MvnI CGCG 1 cut(s) 2141
MwoI GCNNNNNNNGC 7 cut(s) 343, 367, 627, 1907, 2002, 2687, 2744
NaeI GCCGGC 1 cut(s) 372
NdeI CATATG 2 cut(s) 2325, 2677
NdeII GATC 9 cut(s) 201, 606, 1026, 1347, 1726, 2008, 2287, 2308, 2614
NgoMIV GCCGGC 1 cut(s) 370
NlaIV GGNNCC 5 cut(s) 63, 1104, 1314, 1950, 2164
NmeAIII GCCGAG 2 cut(s) 242, 606
NmuCI GTSAC 5 cut(s) 116, 149, 161, 785, 1540
NsiI ATGCAT 2 cut(s) 2017, 2624
NspI RCATGY 2 cut(s) 229, 2626
PaeI GCATGC 1 cut(s) 2626
PceI AGGCCT 2 cut(s) 2340, 2368
PciI ACATGT 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 414
PctI GAATGC 4 cut(s) 2015, 2549, 2560, 2658
PdiI GCCGGC 1 cut(s) 372
PdmI GAANNNNTTC 1 cut(s) 362
PfeI GAWTC 6 cut(s) 273, 505, 1139, 1270, 2181, 2645
PkrI GCNGC 4 cut(s) 323, 1594, 2051, 2628
PleI GAGTC 5 cut(s) 158, 238, 254, 1065, 2399
PpsI GAGTC 5 cut(s) 158, 238, 254, 1065, 2399
Ppu21I YACGTR 1 cut(s) 804
PpuMI RGGWCCY 1 cut(s) 62
PscI ACATGT 1 cut(s) 225
PshAI GACNNNNGTC 1 cut(s) 1167
PshBI ATTAAT 3 cut(s) 819, 1980, 2582
Psp1406I AACGTT 1 cut(s) 1551
Psp5II RGGWCCY 1 cut(s) 62
PspEI GGTNACC 1 cut(s) 1114
PspN4I GGNNCC 5 cut(s) 63, 1104, 1314, 1950, 2164
PspPI GGNCC 4 cut(s) 62, 368, 1313, 2131
PspPPI RGGWCCY 1 cut(s) 62
PstI CTGCAG 1 cut(s) 2495
PsuI RGATCY 1 cut(s) 2614
RsaI GTAC 5 cut(s) 806, 1795, 1808, 1856, 2763
RsaNI GTAC 5 cut(s) 805, 1794, 1807, 1855, 2762
RseI CAYNNNNRTG 2 cut(s) 1130, 1203
SapI GCTCTTC 1 cut(s) 414
SatI GCNGC 4 cut(s) 322, 1593, 2050, 2627
Sau3AI GATC 9 cut(s) 201, 606, 1026, 1347, 1726, 2008, 2287, 2308, 2614
Sau96I GGNCC 4 cut(s) 62, 368, 1313, 2131
ScaI AGTACT 1 cut(s) 1795
SchI GAGTC 5 cut(s) 158, 238, 254, 1065, 2399
SduI GDGCHC 1 cut(s) 2488
SfaNI GCATC 4 cut(s) 1499, 1556, 1579, 1897
SfcI CTRYAG 2 cut(s) 2257, 2491
SinI GGWCC 2 cut(s) 62, 1313
SmiMI CAYNNNNRTG 2 cut(s) 1130, 1203
SmlI CTYRAG 1 cut(s) 2027
SmoI CTYRAG 1 cut(s) 2027
SnaBI TACGTA 1 cut(s) 804
SphI GCATGC 1 cut(s) 2626
Sse9I AATT 8 cut(s) 742, 793, 921, 1128, 1143, 1484, 1767, 2733
SseBI AGGCCT 2 cut(s) 2340, 2368
SsiI CCGC 1 cut(s) 779
SspI AATATT 1 cut(s) 1006
SspMI CTAG 5 cut(s) 1427, 2006, 2213, 2217, 2540
StuI AGGCCT 2 cut(s) 2340, 2368
TaaI ACNGT 9 cut(s) 901, 916, 1086, 1317, 1710, 2077, 2157, 2261, 2578
TaiI ACGT 3 cut(s) 806, 1442, 1554
TaqI TCGA 5 cut(s) 290, 946, 1056, 2586, 2670
TasI AATT 8 cut(s) 742, 793, 921, 1128, 1143, 1484, 1767, 2733
TatI WGTACW 3 cut(s) 1793, 1854, 2761
TfiI GAWTC 6 cut(s) 273, 505, 1139, 1270, 2181, 2645
TscAI CASTG 6 cut(s) 861, 919, 1036, 2162, 2356, 2555
TseFI GTSAC 5 cut(s) 116, 149, 161, 785, 1540
TseI GCWGC 4 cut(s) 321, 1592, 2049, 2626
Tsp45I GTSAC 5 cut(s) 116, 149, 161, 785, 1540
TspRI CASTG 6 cut(s) 861, 919, 1036, 2162, 2356, 2555
Vha464I CTTAAG 1 cut(s) 2027
VpaK11BI GGWCC 2 cut(s) 62, 1313
VspI ATTAAT 3 cut(s) 819, 1980, 2582
XapI RAATTY 3 cut(s) 742, 793, 1484
XceI RCATGY 2 cut(s) 229, 2626
XmiI GTMKAC 1 cut(s) 1442
XmnI GAANNNNTTC 1 cut(s) 362
XspI CTAG 5 cut(s) 1427, 2006, 2213, 2217, 2540
ZrmI AGTACT 1 cut(s) 1795
Zsp2I ATGCAT 2 cut(s) 2017, 2624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.