RLG00000020927

Pentatricopeptide repeat-containing protein At5g65560-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015670
Physical Location & Seq
Forward (+)
70173849 .. 70176629
2781 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020927

Sequence Viewer

Length: 2781 bp
ATGTTAAACCCCATCAAAACCCCAACTCTCCTTCACCTCAGAACCCTCCAATCTCTGTTGGGTCCTTACCATTTTCCCAAACTCTCATCAAGAACCAAGCTTTTCACCCATCCCTGTCACTCCTCCCCAAACCCTTTCCCAAACTTGGTCTCCAAAATCTGTGACATTTTGAGTGACCCCAAATGGGAAAAAAGCTCAGAGCTTTTGGGTCTGATCCACAATCTCAGAACCTATCACATGTTGAAGATTATTCAGACTCACAGGAACACAGACTCGGCTTTGAGATTCTTCTTCTGGGTTTCGAGGAGAAAACCTTACAAGCATGACATGAGCTGCTTTGTTTCAATGCTGGATAGGCTTGTGAGGGAGAAGCTTTTCGGGCCGGCAGACCATGTAAGGCTTTTGCTTGTTAAAGCTTGTAGGAATGAGGAAGAGCTTAAATGGGTGATTGGGTATTTGAATGAAAAGAAAAGAACTGGTTTTGGGTTTACATTGTATAGTTTCAATACGCTTTTGATTCAGTTGGGCAAGTTTGAGATGGTTAGTGAAGCTCAAAATGTGTATAATGAGATACTAAGTAGTGAGGTTAAGCCGAGTCTGTTGACATTTAATACTATGATCAATATATTGTGCAAGAAGGGCAAGGTTCAGGAAGCAGAGTTGATTTTGAACAGGATTATTCAGTATGAAATGTTGCCGGATGTTTTTACTTATACATCGTTGGTTCTTGGGCATTGTAGAAGTGGTAACTTAAATTTGGCATTTGAGGTTTATGACCGGATGGTGAAGGCAGGTTGTGACCCGAATTCGGTTACATACTCAACACTTATTAATGGGTTATGCAATGAGGGGATCGTGAATGAGGCACTGGATATGCTTGATGAAATGATAGGGAAGGGGATTGAACCCACAGTTTATACTTACACTGTCCCAATTACTTCACTTTGTGAGGCAAATCGACCATTGGAAGCAATCAGGCTTTTCAGAAGTATGACGAAGAGGGGTTGCTGTCCGAATATTCATACTTATACAGCATTGATCAGCGGGTTGTCTCGGACAGGGAAACTCGAAGTTGCTATTGGACTCTATCACAAATTGTTGAAGGATGGTATGGTTCCAAGCATGGTTACCTTCAACACAATTATGAATGAATCAATTGAGGCAGGAAGATATGACATGGGTCTGAAGATTATTTATTGGATGGAGAGACATGGTTATGGTATCTCGGAAAATACCCGAACACACAATCATATCATCAAGGGCTTATGCTTGATGGGTAAGATTCACAATGCGATGGCTCTTTTAAGCAAAATGCTTAAAGTGGGACCGTCTCCAAATGTGATTACATATAACACATTGATCAATGGATACCTCAATAGAGGTAATCTAAACAATGCTCTGAGGTTATTAGAGTTGATGAAAGGGAGAGGATGTAAACTAGATGAGTGGACGTATACCGAAGTTATTTCTGGGTGTTGCAAGGCGCACAAGTCTGAATTTGCATCTAATCTTTTCCACGATATGTTAAAACAGGGCATTAGCCCAAATCAGGTCACTTACAACGTTTTGATTGTTGGATGCTGTAATGAGGGGAAGGTAGATGCTGCCTTGTCATTTTTCAAGCAAATGGAAGAGAATGGTTGCTCTCCAGATATTGAAAGCTACAATGCCATTATACATCGTTTGTCGAAAGACAATCAGTTTGCTAAAGCCAAGCAACTGTGTAAAAAGATGGTGGATCAAGGACTGCTTCCAAATGTCATTACCTACACATCTTTAATTGATGGCCTCTGTAAGAATGGTAGTACTGACCTTGCGTTCAGGATTTTCCATGAAATGAGAGAAAGAGATTGCTTGCCGAACTTGTACACTTATAGTTCACTTGTTTTTGGGTTATGTCAAGAAGGCAAGGCTGATGATGCTGAGGTTTTACTTGAAGAAATGGAGAGGGAAGGATTGGTTCCTGATGTGGTAACATTTACCACGCTCATTAATGGTTTCGTTATGCTTGGTAGGCCAGATCATGCATTCTTACTTCTTAAGCGAATGGTTGATGTTGGCTGCAAACCCAACTATTTTACTTTTACTGTGTTGGTCAAAGGGTTGCAAAGGGAAAGTCTGTTACTCACAGAAAAGGTTGTGGGCCTTGTCGCGCAACATGAAGCACAGTGTGGTTCCAGCTCTGACAAGAGATTCAACTTTTTTGAGATATTGTGTAATCTCCTAGCTAGGATGTCAGAGAATGGATGTGAACCAACTATTGATACCTACAGTATTTTAGTGAGAGGCTTGTGTGAAGATTGCAAATATGATGAAGTAGATCAGTTGGTGGAGCATATGAAAGAGAAAGGCCTGTATCCCAGTGAAGAGTTTTACAGGCCTCTATTCTTTGCTCATTGCAAAAACTTAAAACTGGACTCTGCTCTGGAAATCTTTGGCTTGATGGCAGATAGAGGCTTTAAGGTTAACTTTTCAACCTATACAGCACTTATTTGTGCTCTCTGCAGGGCAAATCGGATGGAAGAAGCTGAAACGTTGTTGAAAAGTATGCTAGAATGCCAGTGGAATGCTGATGAGATTGTTTGGACTGTATTAATCGATGGGTTACTGAAAAAAGGGCAGTCAGATCCATGCATGCAGCTTCTTCATGTTATTGAATCTCAAAACTGCATTCTCTGGGTCGAGACATATGCTATCTTGGCCAGAGAACTTTCTCATGTAAATAAAACTATGGCAACTTCTCAAATTGCTGACAGAGCGATAGATTTAGAAGTACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

927

Amino Acids

105.16

Weight (kDa)

7.1

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 136 - 277 8e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 166 - 212 7e-07 PPR repeat family
PPR_1 PF12854 196 - 224 2.3e-07 PPR repeat
PPR_2 PF13041 198 - 247 8.9e-11 PPR repeat family
PPR_1 PF12854 231 - 262 5.7e-09 PPR repeat
PPR_3 PF13812 232 - 278 9.7e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 233 - 282 7.6e-19 PPR repeat family
PPR PF01535 236 - 266 2.6e-06 PPR repeat
TPR_24 PF23276 247 - 365 1e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 265 - 296 1.6e-13 PPR repeat
PPR PF01535 271 - 301 1.7e-07 PPR repeat
PPR_2 PF13041 275 - 316 2.4e-12 PPR repeat family
PPR_3 PF13812 292 - 348 7.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 299 - 332 3.4e-06 PPR repeat
PPR_2 PF13041 312 - 351 4.1e-11 PPR repeat family
PPR_3 PF13812 326 - 383 4.7e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 334 - 363 3.9e-06 PPR repeat
PPR_2 PF13041 342 - 383 2.5e-08 PPR repeat family
PPR_1 PF12854 407 - 438 1.3e-06 PPR repeat
PPR_3 PF13812 407 - 458 1.7e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 411 - 456 7.2e-11 PPR repeat family
PPR_1 PF12854 442 - 474 3e-09 PPR repeat
PPR_2 PF13041 445 - 494 2.1e-17 PPR repeat family
PPR PF01535 448 - 478 4.1e-07 PPR repeat
PPR_long PF17177 462 - 581 3.6e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 468 - 528 5.5e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 484 - 529 5.5e-14 PPR repeat family
TPR_24 PF23276 486 - 578 8.9e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 504 - 560 1.4e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 512 - 544 7.1e-11 PPR repeat
PPR_2 PF13041 516 - 556 8.4e-13 PPR repeat family
PPR PF01535 518 - 548 4.9e-09 PPR repeat
PPR_long PF17177 527 - 698 3.2e-15 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 550 - 599 6.1e-15 PPR repeat family
PPR_3 PF13812 574 - 628 3.8e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 582 - 614 1.5e-13 PPR repeat
PPR_2 PF13041 585 - 634 3.6e-18 PPR repeat family
TPR_24 PF23276 586 - 681 6.1e-06 Fungal tetratrico peptide repeats
PPR PF01535 588 - 618 6.7e-09 PPR repeat
PPR_1 PF12854 617 - 649 1.1e-11 PPR repeat
PPR PF01535 623 - 653 2.2e-06 PPR repeat
PPR_2 PF13041 629 - 668 1.8e-10 PPR repeat family
PPR_3 PF13812 644 - 700 1.5e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 652 - 684 3.5e-08 PPR repeat
PPR_2 PF13041 655 - 702 2.1e-13 PPR repeat family
PPR PF01535 658 - 688 6.9e-06 PPR repeat
PPR_long PF17177 740 - 865 1.4e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 795 - 836 3.3e-07 PPR repeat family
PPR_1 PF12854 818 - 850 1.7e-08 PPR repeat
PPR PF01535 825 - 853 1.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015670)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g41080 FvH4_6g41080 FvH4_6g41080
malus_domestica MD09G1122300.v1.1
prunus_persica Prupe.3G201200_v2.0.a1
pyrus_communis pycom09g04670
rosa_chinensis RchiOBHm_Chr2g0156641
rosa_laevigata RLG00000020927
rosa_multiflora Rmu_sc0018228.1_g000003
rosa_roxburghii Rroxscaffold_2G00092880
rosa_rugosa Rorug02G0454900
rosa_samantha Rh2AG520600 Rh2BG533700 Rh2CG505600 Rh2DG542500
rosa_wichuraiana Rw2G042950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 782
AccI GTMKAC 1 cut(s) 1454
AccII CGCG 1 cut(s) 2153
AciI CCGC 1 cut(s) 1044
AclI AACGTT 2 cut(s) 1563, 2534
AclWI GGATC 4 cut(s) 208, 860, 1746, 2621
AcoI YGGCCR 1 cut(s) 2700
AcsI RAATTY 3 cut(s) 754, 805, 1496
AcuI CTGAAG 1 cut(s) 1205
AdeI CACNNNGTG 2 cut(s) 947, 2171
AfaI GTAC 3 cut(s) 1807, 1868, 2775
AfiI CCNNNNNNNGG 4 cut(s) 145, 184, 808, 1549
AflII CTTAAG 1 cut(s) 2039
AflIII ACRYGT 1 cut(s) 237
AjuI GAANNNNNNNTTGG 2 cut(s) 1062, 1094
Alw21I GWGCWC 1 cut(s) 2500
Alw26I GTCTC 5 cut(s) 154, 1056, 1201, 1335, 2678
AlwI GGATC 4 cut(s) 208, 860, 1746, 2621
AoxI GGCC 7 cut(s) 380, 1786, 2015, 2143, 2350, 2378, 2700
ApeKI GCWGC 4 cut(s) 333, 1604, 2061, 2638
ApoI RAATTY 3 cut(s) 754, 805, 1496
ArsI GACNNNNNNTTYG 4 cut(s) 1685, 1717, 2101, 2133
AseI ATTAAT 3 cut(s) 831, 1992, 2594
Asp700I GAANNNNTTC 1 cut(s) 374
AspLEI GCGC 2 cut(s) 1486, 2155
AspS9I GGNCC 4 cut(s) 62, 380, 1325, 2143
AsuHPI GGTGA 4 cut(s) 26, 97, 457, 796
AvaII GGWCC 2 cut(s) 62, 1325
BalI TGGCCA 1 cut(s) 2702
Bbv12I GWGCWC 1 cut(s) 2500
BbvCI CCTCAGC 1 cut(s) 1923
BbvI GCAGC 4 cut(s) 320, 1591, 2048, 2650
BcgI CGANNNNNNTGC 2 cut(s) 2672, 2706
BciVI GTATCC 2 cut(s) 1361, 2367
BclI TGATCA 3 cut(s) 618, 1038, 1359
BcoDI GTCTC 5 cut(s) 154, 1056, 1201, 1335, 2678
BfaI CTAG 4 cut(s) 1439, 2225, 2229, 2552
BfmI CTRYAG 2 cut(s) 2269, 2503
BfrI CTTAAG 1 cut(s) 2039
BfuAI ACCTGC 1 cut(s) 782
BfuI GTATCC 2 cut(s) 1361, 2367
BisI GCNGC 4 cut(s) 334, 1605, 2062, 2639
BlsI GCNGC 4 cut(s) 335, 1606, 2063, 2640
BmcAI AGTACT 1 cut(s) 1807
Bme18I GGWCC 2 cut(s) 62, 1325
BmgT120I GGNCC 4 cut(s) 62, 380, 1325, 2143
BmiI GGNNCC 5 cut(s) 63, 1116, 1326, 1962, 2176
BmrI ACTGGG 1 cut(s) 2355
BmsI GCATC 4 cut(s) 1511, 1568, 1591, 1909
BmuI ACTGGG 1 cut(s) 2355
BoxI GACNNNNGTC 1 cut(s) 1179
BpmI CTGGAG 1 cut(s) 1632
Bpu10I CCTNAGC 1 cut(s) 1923
Bsa29I ATCGAT 1 cut(s) 2598
BsaI GGTCTC 1 cut(s) 154
BsaWI WCCGGW 1 cut(s) 777
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 1417, 1447
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 184, 808, 1549
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 5 cut(s) 481, 873, 2361, 2418, 2560
Bse3DI GCAATG 2 cut(s) 850, 2395
BseCI ATCGAT 1 cut(s) 2598
BseLI CCNNNNNNNGG 4 cut(s) 145, 184, 808, 1549
BseMI GCAATG 2 cut(s) 850, 2395
BseMII CTCAG 5 cut(s) 52, 210, 238, 1391, 1914
BseNI ACTGG 5 cut(s) 481, 873, 2361, 2418, 2560
BseRI GAGGAG 2 cut(s) 112, 319
BseXI GCAGC 4 cut(s) 320, 1591, 2048, 2650
Bsh1236I CGCG 1 cut(s) 2153
BshFI GGCC 7 cut(s) 382, 1788, 2017, 2145, 2352, 2380, 2702
BshVI ATCGAT 1 cut(s) 2598
BsiHKAI GWGCWC 1 cut(s) 2500
BsiSI CCGG 3 cut(s) 383, 698, 778
BslFI GGGAC 2 cut(s) 914, 1338
BslI CCNNNNNNNGG 4 cut(s) 145, 184, 808, 1549
BsmAI GTCTC 5 cut(s) 154, 1056, 1201, 1335, 2678
BsmBI CGTCTC 1 cut(s) 1335
BsmFI GGGAC 2 cut(s) 914, 1338
BsmI GAATGC 4 cut(s) 2027, 2561, 2572, 2670
BsnI GGCC 7 cut(s) 382, 1788, 2017, 2145, 2352, 2380, 2702
Bso31I GGTCTC 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 2500
Bsp1407I TGTACA 1 cut(s) 1866
Bsp143I GATC 9 cut(s) 213, 618, 852, 1038, 1359, 1738, 2020, 2320, 2626
BspACI CCGC 1 cut(s) 1044
BspANI GGCC 7 cut(s) 382, 1788, 2017, 2145, 2352, 2380, 2702
BspCNI CTCAG 5 cut(s) 51, 209, 237, 1392, 1915
BspDI ATCGAT 1 cut(s) 2598
BspFNI CGCG 1 cut(s) 2153
BspLI GGNNCC 5 cut(s) 63, 1116, 1326, 1962, 2176
BspMAI CTGCAG 1 cut(s) 2507
BspMI ACCTGC 1 cut(s) 782
BspPI GGATC 4 cut(s) 208, 860, 1746, 2621
BspQI GCTCTTC 1 cut(s) 426
BspTI CTTAAG 1 cut(s) 2039
BspTNI GGTCTC 1 cut(s) 154
BsrDI GCAATG 2 cut(s) 850, 2395
BsrFI RCCGGY 1 cut(s) 382
BsrGI TGTACA 1 cut(s) 1866
BsrI ACTGG 5 cut(s) 481, 873, 2361, 2418, 2560
BssAI RCCGGY 1 cut(s) 382
BssMI GATC 9 cut(s) 213, 618, 852, 1038, 1359, 1738, 2020, 2320, 2626
BssNAI GTATAC 1 cut(s) 1455
Bst1107I GTATAC 1 cut(s) 1455
Bst4CI ACNGT 8 cut(s) 913, 928, 1329, 1722, 2089, 2169, 2273, 2590
Bst6I CTCTTC 4 cut(s) 426, 992, 1626, 2361
BstAFI CTTAAG 1 cut(s) 2039
BstAUI TGTACA 1 cut(s) 1866
BstC8I GCNNGC 3 cut(s) 384, 1856, 2636
BstDEI CTNAG 6 cut(s) 38, 196, 224, 575, 1400, 1923
BstEII GGTNACC 1 cut(s) 1126
BstFNI CGCG 1 cut(s) 2153
BstHHI GCGC 2 cut(s) 1486, 2155
BstKTI GATC 9 cut(s) 216, 621, 855, 1041, 1362, 1741, 2023, 2323, 2629
BstMAI GTCTC 5 cut(s) 154, 1056, 1201, 1335, 2678
BstMBI GATC 9 cut(s) 213, 618, 852, 1038, 1359, 1738, 2020, 2320, 2626
BstMWI GCNNNNNNNGC 7 cut(s) 355, 379, 639, 1919, 2014, 2699, 2756
BstNSI RCATGY 2 cut(s) 241, 2638
BstPAI GACNNNNGTC 1 cut(s) 1179
BstPI GGTNACC 1 cut(s) 1126
BstSFI CTRYAG 2 cut(s) 2269, 2503
BstUI CGCG 1 cut(s) 2153
BstV1I GCAGC 4 cut(s) 320, 1591, 2048, 2650
BstX2I RGATCY 1 cut(s) 2626
BstYI RGATCY 1 cut(s) 2626
BstZ17I GTATAC 1 cut(s) 1455
Bsu15I ATCGAT 1 cut(s) 2598
BsuI GTATCC 2 cut(s) 1361, 2367
BsuRI GGCC 7 cut(s) 382, 1788, 2017, 2145, 2352, 2380, 2702
BsuTUI ATCGAT 1 cut(s) 2598
BtgZI GCGATG 1 cut(s) 1307
BtsIMutI CAGTG 5 cut(s) 866, 924, 2174, 2368, 2567
BveI ACCTGC 1 cut(s) 782
Cac8I GCNNGC 3 cut(s) 384, 1856, 2636
CfoI GCGC 2 cut(s) 1486, 2155
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 4 cut(s) 62, 380, 1325, 2143
ClaI ATCGAT 1 cut(s) 2598
Csp6I GTAC 3 cut(s) 1806, 1867, 2774
CviQI GTAC 3 cut(s) 1806, 1867, 2774
DdeI CTNAG 6 cut(s) 38, 196, 224, 575, 1400, 1923
DpnI GATC 9 cut(s) 215, 620, 854, 1040, 1361, 1740, 2022, 2322, 2628
DpnII GATC 9 cut(s) 213, 618, 852, 1038, 1359, 1738, 2020, 2320, 2626
DraIII CACNNNGTG 2 cut(s) 947, 2171
EaeI YGGCCR 1 cut(s) 2700
Eam1104I CTCTTC 4 cut(s) 426, 992, 1626, 2361
EarI CTCTTC 4 cut(s) 426, 992, 1626, 2361
Eco147I AGGCCT 2 cut(s) 2352, 2380
Eco31I GGTCTC 1 cut(s) 154
Eco47I GGWCC 2 cut(s) 62, 1325
Eco57I CTGAAG 1 cut(s) 1205
Eco91I GGTNACC 1 cut(s) 1126
EcoO109I RGGNCCY 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 1126
EcoRI GAATTC 1 cut(s) 805
EcoT22I ATGCAT 2 cut(s) 2029, 2636
Esp3I CGTCTC 1 cut(s) 1335
FalI AAGNNNNNCTT 4 cut(s) 1734, 1766, 2453, 2485
FaqI GGGAC 2 cut(s) 914, 1338
FauI CCCGC 1 cut(s) 1037
FauNDI CATATG 2 cut(s) 2337, 2689
FbaI TGATCA 3 cut(s) 618, 1038, 1359
FblI GTMKAC 1 cut(s) 1454
Fnu4HI GCNGC 4 cut(s) 334, 1605, 2062, 2639
Fsp4HI GCNGC 4 cut(s) 334, 1605, 2062, 2639
FspBI CTAG 4 cut(s) 1439, 2225, 2229, 2552
GlaI GCGC 2 cut(s) 1485, 2154
GluI GCNGC 4 cut(s) 334, 1605, 2062, 2639
GsuI CTGGAG 1 cut(s) 1632
HaeIII GGCC 7 cut(s) 382, 1788, 2017, 2145, 2352, 2380, 2702
HapII CCGG 3 cut(s) 383, 698, 778
HhaI GCGC 2 cut(s) 1486, 2155
Hin6I GCGC 2 cut(s) 1484, 2153
HinP1I GCGC 2 cut(s) 1484, 2153
HincII GTYRAC 2 cut(s) 603, 2467
HindII GTYRAC 2 cut(s) 603, 2467
HindIII AAGCTT 3 cut(s) 98, 371, 414
HpaI GTTAAC 1 cut(s) 2467
HpaII CCGG 3 cut(s) 383, 698, 778
HphI GGTGA 4 cut(s) 26, 97, 457, 796
Hpy166II GTNNAC 9 cut(s) 489, 603, 1436, 1449, 1455, 1869, 1880, 2252, 2467
Hpy188III TCNNGA 9 cut(s) 90, 650, 856, 1649, 1822, 1901, 1964, 2426, 2683
Hpy8I GTNNAC 9 cut(s) 489, 603, 1436, 1449, 1455, 1869, 1880, 2252, 2467
HpyAV CCTTC 9 cut(s) 41, 631, 781, 889, 1096, 1141, 1588, 1898, 1946
HpyCH4III ACNGT 8 cut(s) 913, 928, 1329, 1722, 2089, 2169, 2273, 2590
HpyCH4IV ACGT 3 cut(s) 1451, 1563, 2534
HpyF10VI GCNNNNNNNGC 7 cut(s) 355, 379, 639, 1919, 2014, 2699, 2756
HpyF3I CTNAG 6 cut(s) 38, 196, 224, 575, 1400, 1923
HpySE526I ACGT 3 cut(s) 1451, 1563, 2534
HspAI GCGC 2 cut(s) 1484, 2153
KroI GCCGGC 1 cut(s) 382
KroNI GCCGGC 1 cut(s) 384
Ksp22I TGATCA 3 cut(s) 618, 1038, 1359
KspAI GTTAAC 1 cut(s) 2467
Kzo9I GATC 9 cut(s) 213, 618, 852, 1038, 1359, 1738, 2020, 2320, 2626
LguI GCTCTTC 1 cut(s) 426
LmnI GCTCC 1 cut(s) 2332
Lsp1109I GCAGC 4 cut(s) 320, 1591, 2048, 2650
LweI GCATC 4 cut(s) 1511, 1568, 1591, 1909
MaeI CTAG 4 cut(s) 1439, 2225, 2229, 2552
MaeII ACGT 3 cut(s) 1451, 1563, 2534
MalI GATC 9 cut(s) 215, 620, 854, 1040, 1361, 1740, 2022, 2322, 2628
MboI GATC 9 cut(s) 213, 618, 852, 1038, 1359, 1738, 2020, 2320, 2626
MfeI CAATTG 1 cut(s) 1155
MflI RGATCY 1 cut(s) 2626
MhlI GDGCHC 1 cut(s) 2500
MlsI TGGCCA 1 cut(s) 2702
MluCI AATT 9 cut(s) 754, 805, 933, 1094, 1140, 1155, 1496, 1779, 2745
MluNI TGGCCA 1 cut(s) 2702
MlyI GAGTC 5 cut(s) 250, 266, 604, 1077, 2411
MmeI TCCRAC 1 cut(s) 1555
Mox20I TGGCCA 1 cut(s) 2702
Mph1103I ATGCAT 2 cut(s) 2029, 2636
MroNI GCCGGC 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 374
MscI TGGCCA 1 cut(s) 2702
MslI CAYNNNNRTG 2 cut(s) 1142, 1215
Msp20I TGGCCA 1 cut(s) 2702
MspA1I CMGCKG 1 cut(s) 1044
MspCI CTTAAG 1 cut(s) 2039
MspI CCGG 3 cut(s) 383, 698, 778
MunI CAATTG 1 cut(s) 1155
Mva1269I GAATGC 4 cut(s) 2027, 2561, 2572, 2670
MvnI CGCG 1 cut(s) 2153
MwoI GCNNNNNNNGC 7 cut(s) 355, 379, 639, 1919, 2014, 2699, 2756
NaeI GCCGGC 1 cut(s) 384
NdeI CATATG 2 cut(s) 2337, 2689
NdeII GATC 9 cut(s) 213, 618, 852, 1038, 1359, 1738, 2020, 2320, 2626
NgoMIV GCCGGC 1 cut(s) 382
NlaIV GGNNCC 5 cut(s) 63, 1116, 1326, 1962, 2176
NmeAIII GCCGAG 2 cut(s) 254, 618
NmuCI GTSAC 5 cut(s) 116, 161, 173, 797, 1552
NsiI ATGCAT 2 cut(s) 2029, 2636
NspI RCATGY 2 cut(s) 241, 2638
PaeI GCATGC 1 cut(s) 2638
PceI AGGCCT 2 cut(s) 2352, 2380
PciI ACATGT 1 cut(s) 237
PciSI GCTCTTC 1 cut(s) 426
PctI GAATGC 4 cut(s) 2027, 2561, 2572, 2670
PdiI GCCGGC 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 6 cut(s) 285, 517, 1151, 1282, 2193, 2657
PkrI GCNGC 4 cut(s) 335, 1606, 2063, 2640
PleI GAGTC 5 cut(s) 250, 266, 603, 1077, 2411
PpsI GAGTC 5 cut(s) 250, 266, 603, 1077, 2411
PpuMI RGGWCCY 1 cut(s) 62
PscI ACATGT 1 cut(s) 237
PshAI GACNNNNGTC 1 cut(s) 1179
PshBI ATTAAT 3 cut(s) 831, 1992, 2594
Psp1406I AACGTT 2 cut(s) 1563, 2534
Psp5II RGGWCCY 1 cut(s) 62
PspEI GGTNACC 1 cut(s) 1126
PspN4I GGNNCC 5 cut(s) 63, 1116, 1326, 1962, 2176
PspPI GGNCC 4 cut(s) 62, 380, 1325, 2143
PspPPI RGGWCCY 1 cut(s) 62
PstI CTGCAG 1 cut(s) 2507
PsuI RGATCY 1 cut(s) 2626
RsaI GTAC 3 cut(s) 1807, 1868, 2775
RsaNI GTAC 3 cut(s) 1806, 1867, 2774
RseI CAYNNNNRTG 2 cut(s) 1142, 1215
SapI GCTCTTC 1 cut(s) 426
SatI GCNGC 4 cut(s) 334, 1605, 2062, 2639
Sau3AI GATC 9 cut(s) 213, 618, 852, 1038, 1359, 1738, 2020, 2320, 2626
Sau96I GGNCC 4 cut(s) 62, 380, 1325, 2143
ScaI AGTACT 1 cut(s) 1807
SchI GAGTC 5 cut(s) 250, 266, 604, 1077, 2411
SduI GDGCHC 1 cut(s) 2500
SfaNI GCATC 4 cut(s) 1511, 1568, 1591, 1909
SfcI CTRYAG 2 cut(s) 2269, 2503
SinI GGWCC 2 cut(s) 62, 1325
SmiMI CAYNNNNRTG 2 cut(s) 1142, 1215
SmlI CTYRAG 1 cut(s) 2039
SmoI CTYRAG 1 cut(s) 2039
SphI GCATGC 1 cut(s) 2638
Sse9I AATT 9 cut(s) 754, 805, 933, 1094, 1140, 1155, 1496, 1779, 2745
SseBI AGGCCT 2 cut(s) 2352, 2380
SsiI CCGC 1 cut(s) 1044
SspI AATATT 1 cut(s) 1018
SspMI CTAG 4 cut(s) 1439, 2225, 2229, 2552
StuI AGGCCT 2 cut(s) 2352, 2380
TaaI ACNGT 8 cut(s) 913, 928, 1329, 1722, 2089, 2169, 2273, 2590
TaiI ACGT 3 cut(s) 1454, 1566, 2537
TaqI TCGA 6 cut(s) 302, 958, 1068, 1688, 2598, 2682
TasI AATT 9 cut(s) 754, 805, 933, 1094, 1140, 1155, 1496, 1779, 2745
TatI WGTACW 3 cut(s) 1805, 1866, 2773
TfiI GAWTC 6 cut(s) 285, 517, 1151, 1282, 2193, 2657
TscAI CASTG 5 cut(s) 873, 931, 2174, 2368, 2567
TseFI GTSAC 5 cut(s) 116, 161, 173, 797, 1552
TseI GCWGC 4 cut(s) 333, 1604, 2061, 2638
Tsp45I GTSAC 5 cut(s) 116, 161, 173, 797, 1552
TspRI CASTG 5 cut(s) 873, 931, 2174, 2368, 2567
Vha464I CTTAAG 1 cut(s) 2039
VpaK11BI GGWCC 2 cut(s) 62, 1325
VspI ATTAAT 3 cut(s) 831, 1992, 2594
XapI RAATTY 3 cut(s) 754, 805, 1496
XceI RCATGY 2 cut(s) 241, 2638
XmiI GTMKAC 1 cut(s) 1454
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 4 cut(s) 1439, 2225, 2229, 2552
ZrmI AGTACT 1 cut(s) 1807
Zsp2I ATGCAT 2 cut(s) 2029, 2636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.