pycom09g05840
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007799
Physical Location & Seq
Reverse (-)
4343424 .. 4348766
5343 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2472 bp
ATGTCAAAGCGAAAATCCCCCAACTTGGTTGCATTTCACTCCGGTGATCGTCTTATTGGGGAGGAAGCAGCTGGATTGGTTGCTCGGTACCCGGAAAAAGTCTATTCTCAATCAAGGGACTTGATTGGAAAACCCTTTAGTTCCAGTAAAAGCCTATTGGATTCATTGTACTTGCCATTCGACGTTACGGAGGATACTACGGGAACTGTTAGTTTCAAAATTGATGATAAAGTGACTACTTATTCAGTCGAGGAACTAACGGCGATGGTCTTAGGTTATGCTGCAAATTTGGCAGAGTTTCATTCGAAAGTGCCTGTAAAGGATGCTGTAATTTCAGTTCCTCCATACTTTGGGCAAGCAGAGAGAAAGGGGTTACTCCGAGCGGCACAGTTGGCGGGGATTAATGTTCTTGCTTTGATAAATGAGCATTCCGGTGCAGCATTGCAGTATGGGATTGATAAAGATTTCTCAAATGAGTCGAGGCATATAATTTTCTATGATATGGGCACCAGCAGTACCTATGCAGCACTTGTTTATTTCTCGGCATATAATACCAAGGAGTTTGGTAAGACTGTTTCAGTCAACCAGTTTCAGGTCAAGGATGTCAGATGGGACCCACAACTTGGGGGCCAGAACTTGGAATTACGGTTGGTGGAGCATTTTGCAGATGAGTTTAATAAACAAGTAGGGAATGGTGTTGATGTGAGGAAGTCCCCAAAAGCAATGGCTAAATTGAAGAAACAGGTCAAGCGTACAAAAGAAATTCTAAGTGCAAACAAAATGGCACCAATATCTGTTGAATCCCTTTACGATGATCGGGACTTCAGGAGCACGATAACTCGTGAAAAGTTTGAAGAGCTCTGCGAAGATCTGTGGGAGAAGTCACTTATACCTCTTAAAGAAGTGCTCAAGTATTCTGGTTTAAAGGTAGATGAGATATATGCAGTGGAACTGATAGGAGGAGCTACCAGGGTGCCAAAGCTGCAGGCTAAGCTTCAGGAATATCTTGGAAGGAAAGAGTTGGATAGACATCTGGATGCTGATGAAGCTATAGTTCTAGGTGCAGCATTATATGCTGCTAATTTAAGTGATGGAATCAAGCTGAACCGTAAGTTAGGGATGATTGATGGTTCTACCTATGGGTTTGTGCTTGAGTTAGATGGCATCGATCTTCTGAAAGAAGATATTACTAGGCAGACACTTGTGCAACGGATGAAGAAACTCCCCAGCAAGAGCAAAGATTTCGAAGTGTCGCTAGCGTACGAGAGTGAAGATCTTTTACCCCCAGGTGCCACCTCTCCTGTATTTGCTCAGTACTCTGTGTCCAGTTTGACAGAGACAAGTGAGCAGTATGCATCACGGAATTTGTCTTCACCCATTAAAGCAAGTCTGCATTTCTCTTTGAGTAGAAGTGGTGTTTTGTCGTTGGATCGTGCAGATGCTGTTATTGAAGTGTCAGAATGGGTGGAAGTTCCTAAGAAGAATCTGAGTGTGGAGAATTCAACTAATGTTGCACCGAACATTTCGACTGAAACTGGTGCTCAGAACTCTTCAGAAGACAGCAATGGCAATACTAATGATGGTGGAAATAGTAACACTTCCAACTCTACTGTAGAAGCGGATGTTGTTATTGAAAAAAAGCTGAAAAAGCGGACCTTTAGGATTCCACTGAAGATTGTTGAGAAAACAGTCGGACCTGCAATGTCTCCTTCAAAAGAATCTCTTGCTGAAGCAAAGCGTAAATTAGAAGAATTAGACAAGAAGGATGCAGAGCGGAGGAGAACGGCAGAGTTGAAAAATAACTTGGAAGGATACATATATGGTACTAAAGAAAAGTTTGAAACATCCGAGGAATTTGAAAAAATTTCAACTGGTGAGGAACGCCAATCCTTTATTGGAAAGCTAGATGAGGTGCAAGAGTGGCTTTACACCGATGGTGAAGATGCTACTGCTTCAGAGTTTCAGGAACGCCTAGAGATGCTAAAAGCTATTGGTGATCCAATATTCTTCAGATTCAAAGAGCTTACCGCACGGCCAGAAGCAGTGGAACATGCTCGAAAGTACCTTGTTGAGGTGCAACAGATTCTAAGTGGATGGGAGTCGAACAAACCCTGGATTCCGAAAGATCGAACAGATGAGGTTGCGAGTGATGCTGACAAGTTGAAGAAGTGGTTGGACGAGAGGGAGGCTGAGCAGAAAAAGACACCTGCACATAGCAAACCAGCATTCACATCGGATGAAGTGTTTGGGAAGGTGTTTGATCTGGAAGACAAGGTTGCCAGCGTCAACAGAATCCCCAAGCCAAAGCCTAAAATTGAGAAACCCACAAGCAATGAAACCGAGAGCAGCGGAGAAAAAGCGAAAGATTCTCACTCGAGTTCTGATAATTCTTCACAAGATGACCAGAAAGCCGGAGACTCAGATGACTTAGCAAATGAAAAAGTTGAGTCAGCGGGTCACGATGAGCTATGA

Protein Analysis

824

Amino Acids

91.74

Weight (kDa)

5.27

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 2 - 669 3.1e-89 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2215
Acc36I ACCTGC 2 cut(s) 1705, 2215
Acc65I GGTACC 1 cut(s) 87
AccB1I GGYRCC 5 cut(s) 87, 506, 784, 973, 1289
AccB7I CCANNNNNTGG 3 cut(s) 350, 623, 637
AccBSI CCGCTC 2 cut(s) 383, 1774
AciI CCGC 8 cut(s) 383, 395, 1619, 1651, 1774, 2028, 2349, 2453
AclWI GGATC 2 cut(s) 1437, 1991
AcoI YGGCCR 1 cut(s) 2033
AcsI RAATTY 6 cut(s) 286, 762, 1363, 1498, 1853, 1863
AcuI CTGAAG 7 cut(s) 808, 980, 1536, 1691, 1749, 1938, 1993
AfaI GTAC 8 cut(s) 89, 170, 517, 754, 1262, 1316, 1825, 2063
AfiI CCNNNNNNNGG 6 cut(s) 25, 350, 592, 623, 637, 2071
AjnI CCWGG 3 cut(s) 968, 1285, 2111
AjuI GAANNNNNNNTTGG 4 cut(s) 1787, 1819, 2156, 2188
AleI CACNNNNGTG 1 cut(s) 42
Alw21I GWGCWC 4 cut(s) 833, 861, 909, 1543
Alw26I GTCTC 3 cut(s) 1331, 1710, 2409
AlwI GGATC 2 cut(s) 1437, 1991
AlwNI CAGNNNCTG 1 cut(s) 1442
Ama87I CYCGRG 1 cut(s) 2374
AoxI GGCC 2 cut(s) 628, 2033
ApeKI GCWGC 8 cut(s) 68, 281, 437, 524, 982, 1064, 1076, 2346
ApoI RAATTY 6 cut(s) 286, 762, 1363, 1498, 1853, 1863
AseI ATTAAT 1 cut(s) 402
Asp718I GGTACC 1 cut(s) 87
AspS9I GGNCC 4 cut(s) 613, 628, 1653, 1694
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 5 cut(s) 56, 1365, 1886, 1949, 2006
AsuII TTCGAA 2 cut(s) 305, 1245
AsuNHI GCTAGC 1 cut(s) 1255
AvaI CYCGRG 1 cut(s) 2374
AvaII GGWCC 3 cut(s) 613, 1653, 1694
BaeGI GKGCMC 1 cut(s) 509
BanI GGYRCC 5 cut(s) 87, 506, 784, 973, 1289
BanII GRGCYC 1 cut(s) 861
BarI GAAGNNNNNNTAC 2 cut(s) 1263, 1295
BauI CACGAG 1 cut(s) 840
BbsI GAAGAC 3 cut(s) 1362, 1563, 2274
Bbv12I GWGCWC 4 cut(s) 833, 861, 909, 1543
BbvI GCAGC 8 cut(s) 80, 268, 449, 536, 969, 1063, 1076, 2358
BccI CCATC 8 cut(s) 259, 603, 1085, 1121, 1154, 1574, 1928, 2088
BceAI ACGGC 3 cut(s) 276, 1800, 2048
BcgI CGANNNNNNTGC 4 cut(s) 1225, 1259, 2214, 2248
BciT130I CCWGG 3 cut(s) 970, 1287, 2113
BciVI GTATCC 2 cut(s) 187, 1805
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 3 cut(s) 1331, 1710, 2409
BfaI CTAG 5 cut(s) 1058, 1191, 1256, 1904, 1973
BfmI CTRYAG 3 cut(s) 983, 1050, 1611
BfuAI ACCTGC 2 cut(s) 1705, 2215
BfuI GTATCC 2 cut(s) 187, 1805
BglII AGATCT 2 cut(s) 868, 1273
BisI GCNGC 9 cut(s) 69, 282, 384, 438, 525, 983, 1065, 1077, 2347
BlpI GCTNAGC 2 cut(s) 990, 2190
BlsI GCNGC 9 cut(s) 70, 283, 385, 439, 526, 984, 1066, 1078, 2348
BmcAI AGTACT 1 cut(s) 1316
Bme1390I CCNGG 4 cut(s) 92, 970, 1287, 2113
Bme18I GGWCC 3 cut(s) 613, 1653, 1694
BmeT110I CYCGRG 1 cut(s) 2374
BmgT120I GGNCC 4 cut(s) 613, 628, 1653, 1694
BmiI GGNNCC 8 cut(s) 89, 508, 614, 615, 629, 786, 975, 1291
BmrFI CCNGG 4 cut(s) 92, 970, 1287, 2113
BmsI GCATC 9 cut(s) 313, 1027, 1173, 1364, 1429, 1756, 1933, 1968, 2140
BmtI GCTAGC 1 cut(s) 1259
BpiI GAAGAC 3 cut(s) 1362, 1563, 2274
Bpu1102I GCTNAGC 2 cut(s) 990, 2190
Bpu14I TTCGAA 2 cut(s) 305, 1245
BpuEI CTTGAG 2 cut(s) 893, 1172
BpuMI CCSGG 1 cut(s) 92
Bsa29I ATCGAT 1 cut(s) 1167
BsaBI GATNNNNATC 1 cut(s) 1029
BsaJI CCNNGG 5 cut(s) 555, 969, 1285, 1848, 2111
BsaWI WCCGGW 2 cut(s) 41, 431
Bsc4I CCNNNNNNNGG 6 cut(s) 25, 350, 592, 623, 637, 2071
Bse1I ACTGG 5 cut(s) 144, 586, 1326, 1540, 1876
Bse3DI GCAATG 5 cut(s) 440, 729, 1570, 1707, 2338
Bse8I GATNNNNATC 1 cut(s) 1029
BseBI CCWGG 3 cut(s) 970, 1287, 2113
BseCI ATCGAT 1 cut(s) 1167
BseDI CCNNGG 5 cut(s) 555, 969, 1285, 1848, 2111
BseJI GATNNNNATC 1 cut(s) 1029
BseLI CCNNNNNNNGG 6 cut(s) 25, 350, 592, 623, 637, 2071
BseMI GCAATG 5 cut(s) 440, 729, 1570, 1707, 2338
BseMII CTCAG 5 cut(s) 1325, 1478, 1556, 2181, 2433
BseNI ACTGG 5 cut(s) 144, 586, 1326, 1540, 1876
BseRI GAGGAG 2 cut(s) 975, 1792
BseSI GKGCMC 1 cut(s) 509
BseXI GCAGC 8 cut(s) 80, 268, 449, 536, 969, 1063, 1076, 2358
BseYI CCCAGC 1 cut(s) 1226
BsgI GTGCAG 4 cut(s) 456, 1083, 1455, 2193
BshFI GGCC 2 cut(s) 630, 2035
BshNI GGYRCC 5 cut(s) 87, 506, 784, 973, 1289
BshVI ATCGAT 1 cut(s) 1167
BsiHKAI GWGCWC 4 cut(s) 833, 861, 909, 1543
BsiHKCI CYCGRG 1 cut(s) 2374
BsiSI CCGG 4 cut(s) 42, 92, 432, 2412
BsiWI CGTACG 1 cut(s) 1260
BslFI GGGAC 4 cut(s) 131, 626, 697, 833
BslI CCNNNNNNNGG 6 cut(s) 25, 350, 592, 623, 637, 2071
BsmAI GTCTC 3 cut(s) 1331, 1710, 2409
BsmFI GGGAC 4 cut(s) 131, 626, 697, 833
BsmI GAATGC 2 cut(s) 427, 2225
BsnI GGCC 2 cut(s) 630, 2035
BsoBI CYCGRG 1 cut(s) 2374
Bsp119I TTCGAA 2 cut(s) 305, 1245
Bsp1286I GDGCHC 5 cut(s) 509, 833, 861, 909, 1543
Bsp143I GATC 9 cut(s) 46, 814, 868, 1168, 1273, 1429, 1996, 2125, 2260
Bsp1720I GCTNAGC 2 cut(s) 990, 2190
BspACI CCGC 8 cut(s) 383, 395, 1619, 1651, 1774, 2028, 2349, 2453
BspANI GGCC 2 cut(s) 630, 2035
BspCNI CTCAG 5 cut(s) 1324, 1479, 1555, 2182, 2432
BspDI ATCGAT 1 cut(s) 1167
BspLI GGNNCC 8 cut(s) 89, 508, 614, 615, 629, 786, 975, 1291
BspMAI CTGCAG 1 cut(s) 987
BspMI ACCTGC 2 cut(s) 1705, 2215
BspOI GCTAGC 1 cut(s) 1259
BspPI GGATC 2 cut(s) 1437, 1991
BspQI GCTCTTC 1 cut(s) 849
BspT104I TTCGAA 2 cut(s) 305, 1245
BspT107I GGYRCC 5 cut(s) 87, 506, 784, 973, 1289
BsrBI CCGCTC 2 cut(s) 383, 1774
BsrDI GCAATG 5 cut(s) 440, 729, 1570, 1707, 2338
BsrI ACTGG 5 cut(s) 144, 586, 1326, 1540, 1876
BssECI CCNNGG 5 cut(s) 555, 969, 1285, 1848, 2111
BssMI GATC 9 cut(s) 46, 814, 868, 1168, 1273, 1429, 1996, 2125, 2260
BssSI CACGAG 1 cut(s) 840
BssT1I CCWWGG 1 cut(s) 555
Bst2BI CACGAG 1 cut(s) 840
Bst2UI CCWGG 3 cut(s) 970, 1287, 2113
Bst4CI ACNGT 7 cut(s) 208, 390, 574, 648, 1109, 1612, 1690
Bst6I CTCTTC 2 cut(s) 849, 1555
BstAPI GCANNNNNTGC 1 cut(s) 1073
BstBI TTCGAA 2 cut(s) 305, 1245
BstC8I GCNNGC 4 cut(s) 357, 987, 1257, 2281
BstENI CCTNNNNNAGG 1 cut(s) 2069
BstKTI GATC 9 cut(s) 49, 817, 871, 1171, 1276, 1432, 1999, 2128, 2263
BstMAI GTCTC 3 cut(s) 1331, 1710, 2409
BstMBI GATC 9 cut(s) 46, 814, 868, 1168, 1273, 1429, 1996, 2125, 2260
BstMWI GCNNNNNNNGC 9 cut(s) 290, 392, 982, 991, 1046, 1073, 1648, 1921, 2150
BstNI CCWGG 3 cut(s) 970, 1287, 2113
BstNSI RCATGY 1 cut(s) 2054
BstSCI CCNGG 4 cut(s) 90, 968, 1285, 2111
BstSFI CTRYAG 3 cut(s) 983, 1050, 1611
BstSLI GKGCMC 1 cut(s) 509
BstV1I GCAGC 8 cut(s) 80, 268, 449, 536, 969, 1063, 1076, 2358
BstV2I GAAGAC 3 cut(s) 1362, 1563, 2274
BstX2I RGATCY 2 cut(s) 868, 1273
BstYI RGATCY 2 cut(s) 868, 1273
Bsu15I ATCGAT 1 cut(s) 1167
BsuI GTATCC 2 cut(s) 187, 1805
BsuRI GGCC 2 cut(s) 630, 2035
BsuTUI ATCGAT 1 cut(s) 1167
BtgZI GCGATG 1 cut(s) 278
BtsI GCAGTG 2 cut(s) 951, 2049
BtsIMutI CAGTG 3 cut(s) 951, 1667, 2049
BveI ACCTGC 2 cut(s) 1705, 2215
Cac8I GCNNGC 4 cut(s) 357, 987, 1257, 2281
CaiI CAGNNNCTG 1 cut(s) 1442
Cfr13I GGNCC 4 cut(s) 613, 628, 1653, 1694
ClaI ATCGAT 1 cut(s) 1167
CseI GACGC 1 cut(s) 2272
Csp6I GTAC 8 cut(s) 88, 169, 516, 753, 1261, 1315, 1824, 2062
CviAII CATG 1 cut(s) 2051
CviQI GTAC 8 cut(s) 88, 169, 516, 753, 1261, 1315, 1824, 2062
DpnI GATC 9 cut(s) 48, 816, 870, 1170, 1275, 1431, 1998, 2127, 2262
DpnII GATC 9 cut(s) 46, 814, 868, 1168, 1273, 1429, 1996, 2125, 2260
DraI TTTAAA 1 cut(s) 924
EaeI YGGCCR 1 cut(s) 2033
Eam1104I CTCTTC 2 cut(s) 849, 1555
EarI CTCTTC 2 cut(s) 849, 1555
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 861
Eco47I GGWCC 3 cut(s) 613, 1653, 1694
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 7 cut(s) 808, 980, 1536, 1691, 1749, 1938, 1993
Eco88I CYCGRG 1 cut(s) 2374
EcoICRI GAGCTC 1 cut(s) 859
EcoNI CCTNNNNNAGG 1 cut(s) 2069
EcoO109I RGGNCCY 1 cut(s) 613
EcoRI GAATTC 1 cut(s) 1498
EcoRII CCWGG 3 cut(s) 968, 1285, 2111
EcoT14I CCWWGG 1 cut(s) 555
EcoT22I ATGCAT 1 cut(s) 1357
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 1 cut(s) 2054
FalI AAGNNNNNCTT 6 cut(s) 1640, 1672, 1707, 1739, 1908, 1940
FaqI GGGAC 4 cut(s) 131, 626, 697, 833
FatI CATG 1 cut(s) 2050
FauI CCCGC 2 cut(s) 388, 2446
Fnu4HI GCNGC 9 cut(s) 69, 282, 384, 438, 525, 983, 1065, 1077, 2347
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 9 cut(s) 69, 282, 384, 438, 525, 983, 1065, 1077, 2347
FspBI CTAG 5 cut(s) 1058, 1191, 1256, 1904, 1973
GluI GCNGC 9 cut(s) 69, 282, 384, 438, 525, 983, 1065, 1077, 2347
GsaI CCCAGC 1 cut(s) 1230
HaeIII GGCC 2 cut(s) 630, 2035
HapII CCGG 4 cut(s) 42, 92, 432, 2412
HgaI GACGC 1 cut(s) 2272
Hin1II CATG 1 cut(s) 2054
HincII GTYRAC 2 cut(s) 583, 2287
HindII GTYRAC 2 cut(s) 583, 2287
HindIII AAGCTT 1 cut(s) 992
HpaII CCGG 4 cut(s) 42, 92, 432, 2412
HphI GGTGA 5 cut(s) 56, 1365, 1886, 1949, 2006
Hpy166II GTNNAC 2 cut(s) 583, 2287
Hpy188III TCNNGA 8 cut(s) 818, 826, 842, 998, 1034, 1964, 2264, 2459
Hpy8I GTNNAC 2 cut(s) 583, 2287
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 5 cut(s) 1005, 1719, 1756, 1802, 2245
HpyCH4III ACNGT 7 cut(s) 208, 390, 574, 648, 1109, 1612, 1690
HpyCH4IV ACGT 1 cut(s) 183
HpyF10VI GCNNNNNNNGC 9 cut(s) 290, 392, 982, 991, 1046, 1073, 1648, 1921, 2150
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 1 cut(s) 2054
KflI GGGWCCC 1 cut(s) 613
KpnI GGTACC 1 cut(s) 91
Kzo9I GATC 9 cut(s) 46, 814, 868, 1168, 1273, 1429, 1996, 2125, 2260
LguI GCTCTTC 1 cut(s) 849
LmnI GCTCC 3 cut(s) 655, 828, 962
Lsp1109I GCAGC 8 cut(s) 80, 268, 449, 536, 969, 1063, 1076, 2358
LweI GCATC 9 cut(s) 313, 1027, 1173, 1364, 1429, 1756, 1933, 1968, 2140
MaeI CTAG 5 cut(s) 1058, 1191, 1256, 1904, 1973
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 6 cut(s) 184, 232, 372, 882, 1592, 2456
MalI GATC 9 cut(s) 48, 816, 870, 1170, 1275, 1431, 1998, 2127, 2262
MbiI CCGCTC 2 cut(s) 383, 1774
MboI GATC 9 cut(s) 46, 814, 868, 1168, 1273, 1429, 1996, 2125, 2260
MflI RGATCY 2 cut(s) 868, 1273
MhlI GDGCHC 5 cut(s) 509, 833, 861, 909, 1543
MlyI GAGTC 4 cut(s) 485, 2108, 2411, 2456
MmeI TCCRAC 5 cut(s) 1002, 1407, 1626, 1672, 2154
Mph1103I ATGCAT 1 cut(s) 1357
MseI TTAA 6 cut(s) 402, 675, 897, 923, 1085, 1380
MslI CAYNNNNRTG 3 cut(s) 42, 432, 1035
MspA1I CMGCKG 3 cut(s) 71, 2349, 2453
MspI CCGG 4 cut(s) 42, 92, 432, 2412
MspR9I CCNGG 4 cut(s) 92, 970, 1287, 2113
Mva1269I GAATGC 2 cut(s) 427, 2225
MvaI CCWGG 3 cut(s) 970, 1287, 2113
MwoI GCNNNNNNNGC 9 cut(s) 290, 392, 982, 991, 1046, 1073, 1648, 1921, 2150
NciI CCSGG 1 cut(s) 92
NdeII GATC 9 cut(s) 46, 814, 868, 1168, 1273, 1429, 1996, 2125, 2260
NheI GCTAGC 1 cut(s) 1255
NlaIII CATG 1 cut(s) 2054
NlaIV GGNNCC 8 cut(s) 89, 508, 614, 615, 629, 786, 975, 1291
NmeAIII GCCGAG 1 cut(s) 521
NmuCI GTSAC 3 cut(s) 232, 882, 2456
NsiI ATGCAT 1 cut(s) 1357
NspI RCATGY 1 cut(s) 2054
NspV TTCGAA 2 cut(s) 305, 1245
OliI CACNNNNGTG 1 cut(s) 42
PaeR7I CTCGAG 1 cut(s) 2374
PaqCI CACCTGC 1 cut(s) 2215
PciSI GCTCTTC 1 cut(s) 849
PctI GAATGC 2 cut(s) 427, 2225
Pfl23II CGTACG 1 cut(s) 1260
PflMI CCANNNNNTGG 3 cut(s) 350, 623, 637
PkrI GCNGC 9 cut(s) 70, 283, 385, 439, 526, 984, 1066, 1078, 2348
PleI GAGTC 4 cut(s) 484, 2107, 2411, 2455
PpsI GAGTC 4 cut(s) 484, 2107, 2411, 2455
PpuMI RGGWCCY 1 cut(s) 613
PshBI ATTAAT 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 861
Psp5II RGGWCCY 1 cut(s) 613
Psp6I CCWGG 3 cut(s) 968, 1285, 2111
PspFI CCCAGC 1 cut(s) 1226
PspGI CCWGG 3 cut(s) 968, 1285, 2111
PspLI CGTACG 1 cut(s) 1260
PspN4I GGNNCC 8 cut(s) 89, 508, 614, 615, 629, 786, 975, 1291
PspPI GGNCC 4 cut(s) 613, 628, 1653, 1694
PspPPI RGGWCCY 1 cut(s) 613
PspXI VCTCGAGB 1 cut(s) 2374
PsrI GAACNNNNNNTAC 2 cut(s) 321, 353
PstI CTGCAG 1 cut(s) 987
PstNI CAGNNNCTG 1 cut(s) 1442
PsuI RGATCY 2 cut(s) 868, 1273
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 8 cut(s) 89, 170, 517, 754, 1262, 1316, 1825, 2063
RsaNI GTAC 8 cut(s) 88, 169, 516, 753, 1261, 1315, 1824, 2062
RseI CAYNNNNRTG 3 cut(s) 42, 432, 1035
SacI GAGCTC 1 cut(s) 861
SapI GCTCTTC 1 cut(s) 849
SaqAI TTAA 6 cut(s) 402, 675, 897, 923, 1085, 1380
SatI GCNGC 9 cut(s) 69, 282, 384, 438, 525, 983, 1065, 1077, 2347
Sau3AI GATC 9 cut(s) 46, 814, 868, 1168, 1273, 1429, 1996, 2125, 2260
Sau96I GGNCC 4 cut(s) 613, 628, 1653, 1694
ScaI AGTACT 1 cut(s) 1316
SchI GAGTC 4 cut(s) 485, 2108, 2411, 2456
ScrFI CCNGG 4 cut(s) 92, 970, 1287, 2113
SduI GDGCHC 5 cut(s) 509, 833, 861, 909, 1543
SfaNI GCATC 9 cut(s) 313, 1027, 1173, 1364, 1429, 1756, 1933, 1968, 2140
SfcI CTRYAG 3 cut(s) 983, 1050, 1611
Sfr274I CTCGAG 1 cut(s) 2374
SfuI TTCGAA 2 cut(s) 305, 1245
SinI GGWCC 3 cut(s) 613, 1653, 1694
SlaI CTCGAG 1 cut(s) 2374
SmiMI CAYNNNNRTG 3 cut(s) 42, 432, 1035
SmlI CTYRAG 3 cut(s) 908, 1151, 2374
SmoI CTYRAG 3 cut(s) 908, 1151, 2374
SsiI CCGC 8 cut(s) 383, 395, 1619, 1651, 1774, 2028, 2349, 2453
SspI AATATT 1 cut(s) 2004
SspMI CTAG 5 cut(s) 1058, 1191, 1256, 1904, 1973
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 4 cut(s) 90, 968, 1285, 2111
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 7 cut(s) 208, 390, 574, 648, 1109, 1612, 1690
TaiI ACGT 1 cut(s) 186
TatI WGTACW 2 cut(s) 168, 1314
TauI GCSGC 1 cut(s) 386
Tru1I TTAA 6 cut(s) 402, 675, 897, 923, 1085, 1380
Tru9I TTAA 6 cut(s) 402, 675, 897, 923, 1085, 1380
TscAI CASTG 3 cut(s) 951, 1674, 2049
TseFI GTSAC 3 cut(s) 232, 882, 2456
TseI GCWGC 8 cut(s) 68, 281, 437, 524, 982, 1064, 1076, 2346
Tsp45I GTSAC 3 cut(s) 232, 882, 2456
TspDTI ATGAA 7 cut(s) 153, 290, 1059, 1229, 2253, 2349, 2451
TspGWI ACGGA 3 cut(s) 203, 1225, 1375
TspRI CASTG 3 cut(s) 951, 1674, 2049
Van91I CCANNNNNTGG 3 cut(s) 350, 623, 637
VpaK11BI GGWCC 3 cut(s) 613, 1653, 1694
VspI ATTAAT 1 cut(s) 402
XagI CCTNNNNNAGG 1 cut(s) 2069
XapI RAATTY 6 cut(s) 286, 762, 1363, 1498, 1853, 1863
XceI RCATGY 1 cut(s) 2054
XcmI CCANNNNNNNNNTGG 1 cut(s) 1892
XhoI CTCGAG 1 cut(s) 2374
XspI CTAG 5 cut(s) 1058, 1191, 1256, 1904, 1973
ZrmI AGTACT 1 cut(s) 1316
Zsp2I ATGCAT 1 cut(s) 1357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.