pycom09g08250

V-type proton ATPase catalytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0008079
Physical Location & Seq
Reverse (-)
6320186 .. 6324400
4215 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1518 bp
ATGCCGGCGGTATACGGTGCCCGATTGACCACTTTTGAGGACTCCGAGAAGGAGAGTGAGTATGGCTACGTCCGCAAGGTCTCTGGACCAGTCGTGGTTGCTGATGGCATGGCTGGAGCTGCTATGTACGAGTTGGTTCGTGTTGGACATGATAATTTGATTGGTGAAATTATTCGGTTAGAAGGAGATTCTGCCACAATTCAAGTTTATGAGGAAACAGCTGGGTTGATGGTGATCGATCCGGTTCTACGTACTCGCAAGCCTTTATCAGTGGAGTTGGGACCTGGAATATTGGGAAATATTTTTGACGGAATCCAGAGGCCCTTGAAAACTATTGCAAAGATCTCTGGTGATGTCTATATCCCACGTGGTGTATCTGTCCCTGCTCTTGACAAAGATATACTTTGGGAGTTTCAACCTAAAAAAATTGGTGAAGGTGATCATCTTACTGGTGGAGACTTGTACGCTACGGTCTTCGAAAATAGTCTGATGGAGCACCGAGTTGCACTTCCTCCCGATGCTATGGGAAAAGTTACTTACGTCGCACCACCTGGTCAATATTCATTGAAGGATACTGTGTTAGAGCTTGAGTTTCAAGGACAAAAAAAGAAATTTACAATGCTTCAGACTTGGCCTGTACGTACACCTAGGCCTGTTGCATCAAAGCTTGCTGCTGATACCCCTCTACTCACTGGGCAGCGTGTTCTTGATGCCCTTTTCCCCTCTGTTCTTGGTGGGACTTGTGCCATTCCTGGGGCTTTTGGTTGTGGAAAAACGGTTATTAGTCAAGCTCTTTCAAAGTACTCAAACTCAGACACTGTGGTTTATGTTGGTTGTGGAGAACGTGGAAATGAAATGGCAGAGGTGCTTATGGATTTCCCTCAATTGACAATGACTTTGCCTGATGGTCGTGAAGAATCTGTCATGAAGCGCACAACCCTTGTGGCCAACACTTCTAACATGCCTGTGGCCGCTCGTGAAGCTTCAATCTATACTGGAATCACTATTGCTGAATACTTCAGAGATATGGGCTACAATGTCAGCATGATGGCAGATTCAACATCTCGATGGGCTGAGGCATTGCGTGAGATTTCTGGGCGGCTGGCTGAAATGCCTGCAGATAGTGGATACCCTGCTTATTTGGCAGCACGTTTAGCATCATTTTACGAACGGGCTGGTAAAGTAAAATGTCTTGGTGGGCCAGAGCGTAATGGCAGTGTGACAATTGTTGGTGCTGTTTCTCCTCCAGGAGGAGATTTCTCAGACCCTGTGACATCTGCAACCCTTAGCATTGTTCAGGTTTTCTGGGGTCTGGACAAAAAACTTGCCCAGAGGAAGCATTTCCCCTCTGTGAACTGGCTTATTTCTTACTCGAAGTACTCAACGGCATTAGAGTCTTTCTATGATCAATTCGATCCTGATTTTATCAACATCAGGACAAAGGCACGTGAGGTTTTGCAACGAGAAGATCGATCTCAACGAAATTGTCCAACTTGTAGGAAAGGATGCTTTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0002020 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006950 GO:0006970 GO:0006996 GO:0007030 GO:0007275 GO:0008150 GO:0008324 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009555 GO:0009628 GO:0009651 GO:0009941 GO:0009987 GO:0010256 GO:0012505 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019899 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030054 GO:0030312 GO:0031090 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0034220 GO:0036442 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0046961 GO:0048046 GO:0048229 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071840 GO:0071944 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04145 ko04150 ko04721 ko04966 ko05110 ko05120 ko05323 map00190 map01100 map04145 map04150 map04721 map04966 map05110 map05120 map05323
Pfam Domains
Protein Families
ATP-synt_ab ATP-synt_ab_N ATP-synt_ab_Xtn

Protein Analysis

506

Amino Acids

55.17

Weight (kDa)

5.83

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_ab_N PF02874 25 - 85 3.9e-14 ATP synthase alpha/beta family, beta-barrel domain
ATP-synt_ab_Xtn PF16886 127 - 214 7.2e-29 ATPsynthase alpha/beta subunit barrel-sandwich domain
ATP-synt_ab PF00006 232 - 458 5.6e-75 ATP synthase alpha/beta family, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 17
AccBSI CCGCTC 1 cut(s) 974
AccI GTMKAC 1 cut(s) 12
AciI CCGC 4 cut(s) 8, 73, 972, 1099
AclWI GGATC 2 cut(s) 233, 1409
AcoI YGGCCR 2 cut(s) 945, 969
AcsI RAATTY 1 cut(s) 611
AcuI CTGAAG 2 cut(s) 608, 1003
AcvI CACGTG 2 cut(s) 368, 1448
AdeI CACNNNGTG 1 cut(s) 371
AfaI GTAC 7 cut(s) 128, 253, 464, 639, 643, 803, 1379
AfiI CCNNNNNNNGG 2 cut(s) 753, 1250
AgsI TTSAA 8 cut(s) 203, 328, 416, 568, 596, 798, 987, 1059
AjnI CCWGG 4 cut(s) 283, 550, 751, 1246
AluBI AGCT 6 cut(s) 119, 221, 586, 667, 791, 983
AluI AGCT 6 cut(s) 119, 221, 586, 667, 791, 983
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 2 cut(s) 85, 450
AlwI GGATC 2 cut(s) 233, 1409
AlwNI CAGNNNCTG 2 cut(s) 818, 1268
AoxI GGCC 6 cut(s) 320, 632, 650, 945, 969, 1199
ApeKI GCWGC 4 cut(s) 119, 671, 697, 1145
ApoI RAATTY 1 cut(s) 611
ArsI GACNNNNNNTTYG 2 cut(s) 880, 912
Asp700I GAANNNNTTC 2 cut(s) 171, 1340
AspA2I CCTAGG 1 cut(s) 647
AspLEI GCGC 1 cut(s) 933
AspS9I GGNCC 4 cut(s) 86, 281, 321, 1199
AsuHPI GGTGA 5 cut(s) 176, 244, 362, 443, 449
AsuII TTCGAA 1 cut(s) 477
AvaII GGWCC 2 cut(s) 86, 281
AvrII CCTAGG 1 cut(s) 647
BaeGI GKGCMC 1 cut(s) 22
BalI TGGCCA 1 cut(s) 947
BanI GGYRCC 1 cut(s) 17
BauI CACGAG 1 cut(s) 975
BbrPI CACGTG 2 cut(s) 368, 1448
BbsI GAAGAC 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 498
BbvCI CCTCAGC 1 cut(s) 1074
BbvI GCAGC 4 cut(s) 106, 658, 709, 1157
BccI CCATC 6 cut(s) 98, 223, 484, 899, 1042, 1062
BceAI ACGGC 1 cut(s) 1401
BciT130I CCWGG 4 cut(s) 285, 552, 753, 1248
BciVI GTATCC 2 cut(s) 565, 1121
BclI TGATCA 2 cut(s) 439, 1405
BcoDI GTCTC 2 cut(s) 85, 450
BfaI CTAG 1 cut(s) 648
BfmI CTRYAG 1 cut(s) 1116
BfuI GTATCC 2 cut(s) 565, 1121
BglII AGATCT 1 cut(s) 342
BisI GCNGC 6 cut(s) 120, 672, 698, 972, 1100, 1146
BlnI CCTAGG 1 cut(s) 647
BlsI GCNGC 6 cut(s) 121, 673, 699, 973, 1101, 1147
BmcAI AGTACT 2 cut(s) 803, 1379
Bme1390I CCNGG 4 cut(s) 285, 552, 753, 1248
Bme18I GGWCC 2 cut(s) 86, 281
BmgT120I GGNCC 4 cut(s) 86, 281, 321, 1199
BmiI GGNNCC 2 cut(s) 19, 282
BmrFI CCNGG 4 cut(s) 285, 552, 753, 1248
BmrI ACTGGG 1 cut(s) 702
BmsI GCATC 5 cut(s) 508, 668, 700, 1166, 1496
BmuI ACTGGG 1 cut(s) 702
BpiI GAAGAC 1 cut(s) 466
BpmI CTGGAG 2 cut(s) 135, 1230
Bpu10I CCTNAGC 2 cut(s) 1074, 1286
Bpu14I TTCGAA 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 608
Bsa29I ATCGAT 2 cut(s) 237, 1471
BsaAI YACGTR 4 cut(s) 251, 368, 641, 1448
BsaBI GATNNNNATC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 647, 752
BsaWI WCCGGW 1 cut(s) 241
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 2 cut(s) 753, 1250
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 5 cut(s) 89, 454, 697, 1000, 1361
Bse3DI GCAATG 1 cut(s) 1079
Bse8I GATNNNNATC 1 cut(s) 233
BseBI CCWGG 4 cut(s) 285, 552, 753, 1248
BseCI ATCGAT 2 cut(s) 237, 1471
BseDI CCNNGG 2 cut(s) 647, 752
BseGI GGATG 1 cut(s) 1511
BseJI GATNNNNATC 1 cut(s) 233
BseLI CCNNNNNNNGG 2 cut(s) 753, 1250
BseMI GCAATG 1 cut(s) 1079
BseMII CTCAG 3 cut(s) 825, 1065, 1275
BseNI ACTGG 5 cut(s) 89, 454, 697, 1000, 1361
BseRI GAGGAG 2 cut(s) 1233, 1266
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 4 cut(s) 106, 658, 709, 1157
BseYI CCCAGC 1 cut(s) 221
BshFI GGCC 6 cut(s) 322, 634, 652, 947, 971, 1201
BshNI GGYRCC 1 cut(s) 17
BshVI ATCGAT 2 cut(s) 237, 1471
BsiHKAI GWGCWC 1 cut(s) 498
BsiSI CCGG 2 cut(s) 5, 242
BslFI GGGAC 3 cut(s) 294, 365, 751
BslI CCNNNNNNNGG 2 cut(s) 753, 1250
BsmAI GTCTC 2 cut(s) 85, 450
BsmFI GGGAC 3 cut(s) 294, 365, 751
BsnI GGCC 6 cut(s) 322, 634, 652, 947, 971, 1201
Bso31I GGTCTC 1 cut(s) 85
Bsp119I TTCGAA 1 cut(s) 477
Bsp1286I GDGCHC 2 cut(s) 22, 498
Bsp143I GATC 8 cut(s) 234, 238, 342, 439, 1405, 1414, 1468, 1472
BspACI CCGC 4 cut(s) 8, 73, 972, 1099
BspANI GGCC 6 cut(s) 322, 634, 652, 947, 971, 1201
BspCNI CTCAG 3 cut(s) 824, 1066, 1274
BspDI ATCGAT 2 cut(s) 237, 1471
BspHI TCATGA 1 cut(s) 924
BspLI GGNNCC 2 cut(s) 19, 282
BspMAI CTGCAG 1 cut(s) 1120
BspPI GGATC 2 cut(s) 233, 1409
BspT104I TTCGAA 1 cut(s) 477
BspT107I GGYRCC 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 85
BsrBI CCGCTC 1 cut(s) 974
BsrDI GCAATG 1 cut(s) 1079
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 5 cut(s) 89, 454, 697, 1000, 1361
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 647, 752
BssMI GATC 8 cut(s) 234, 238, 342, 439, 1405, 1414, 1468, 1472
BssNAI GTATAC 1 cut(s) 13
BssSI CACGAG 1 cut(s) 975
BssT1I CCWWGG 1 cut(s) 647
Bst1107I GTATAC 1 cut(s) 13
Bst2BI CACGAG 1 cut(s) 975
Bst2UI CCWGG 4 cut(s) 285, 552, 753, 1248
Bst4CI ACNGT 5 cut(s) 17, 472, 577, 778, 820
BstBAI YACGTR 4 cut(s) 251, 368, 641, 1448
BstBI TTCGAA 1 cut(s) 477
BstC8I GCNNGC 5 cut(s) 6, 260, 669, 1104, 1116
BstDEI CTNAG 4 cut(s) 811, 1074, 1261, 1286
BstENI CCTNNNNNAGG 1 cut(s) 1248
BstF5I GGATG 1 cut(s) 1511
BstHHI GCGC 1 cut(s) 933
BstKTI GATC 8 cut(s) 237, 241, 345, 442, 1408, 1417, 1471, 1475
BstMAI GTCTC 2 cut(s) 85, 450
BstMBI GATC 8 cut(s) 234, 238, 342, 439, 1405, 1414, 1468, 1472
BstMWI GCNNNNNNNGC 5 cut(s) 72, 119, 980, 1142, 1154
BstNI CCWGG 4 cut(s) 285, 552, 753, 1248
BstNSI RCATGY 1 cut(s) 964
BstSCI CCNGG 4 cut(s) 283, 550, 751, 1246
BstSFI CTRYAG 1 cut(s) 1116
BstSLI GKGCMC 1 cut(s) 22
BstSNI TACGTA 2 cut(s) 251, 641
BstV1I GCAGC 4 cut(s) 106, 658, 709, 1157
BstV2I GAAGAC 1 cut(s) 466
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
BstZ17I GTATAC 1 cut(s) 13
Bsu15I ATCGAT 2 cut(s) 237, 1471
BsuI GTATCC 2 cut(s) 565, 1121
BsuRI GGCC 6 cut(s) 322, 634, 652, 947, 971, 1201
BsuTUI ATCGAT 2 cut(s) 237, 1471
BtsCI GGATG 1 cut(s) 1511
BtsI GCAGTG 1 cut(s) 1222
BtsIMutI CAGTG 4 cut(s) 276, 690, 816, 1222
Cac8I GCNNGC 5 cut(s) 6, 260, 669, 1104, 1116
CaiI CAGNNNCTG 2 cut(s) 818, 1268
CciI TCATGA 1 cut(s) 924
CfoI GCGC 1 cut(s) 933
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 4 cut(s) 86, 281, 321, 1199
ClaI ATCGAT 2 cut(s) 237, 1471
CsiI ACCWGGT 1 cut(s) 550
Csp6I GTAC 7 cut(s) 127, 252, 463, 638, 642, 802, 1378
CspCI CAANNNNNGTGG 2 cut(s) 924, 959
CviAII CATG 5 cut(s) 109, 149, 925, 961, 1045
CviQI GTAC 7 cut(s) 127, 252, 463, 638, 642, 802, 1378
DdeI CTNAG 4 cut(s) 811, 1074, 1261, 1286
DpnI GATC 8 cut(s) 236, 240, 344, 441, 1407, 1416, 1470, 1474
DpnII GATC 8 cut(s) 234, 238, 342, 439, 1405, 1414, 1468, 1472
DraIII CACNNNGTG 1 cut(s) 371
EaeI YGGCCR 2 cut(s) 945, 969
Eco105I TACGTA 2 cut(s) 251, 641
Eco130I CCWWGG 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 652
Eco31I GGTCTC 1 cut(s) 85
Eco47I GGWCC 2 cut(s) 86, 281
Eco57I CTGAAG 2 cut(s) 608, 1003
Eco72I CACGTG 2 cut(s) 368, 1448
EcoNI CCTNNNNNAGG 1 cut(s) 1248
EcoO109I RGGNCCY 2 cut(s) 281, 321
EcoRII CCWGG 4 cut(s) 283, 550, 751, 1246
EcoT14I CCWWGG 1 cut(s) 647
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 5 cut(s) 112, 152, 928, 964, 1048
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FaqI GGGAC 3 cut(s) 294, 365, 751
FatI CATG 5 cut(s) 108, 148, 924, 960, 1044
FbaI TGATCA 2 cut(s) 439, 1405
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 6 cut(s) 120, 672, 698, 972, 1100, 1146
Fsp4HI GCNGC 6 cut(s) 120, 672, 698, 972, 1100, 1146
FspBI CTAG 1 cut(s) 648
GlaI GCGC 1 cut(s) 932
GluI GCNGC 6 cut(s) 120, 672, 698, 972, 1100, 1146
GsaI CCCAGC 1 cut(s) 225
GsuI CTGGAG 2 cut(s) 135, 1230
HaeIII GGCC 6 cut(s) 322, 634, 652, 947, 971, 1201
HapII CCGG 2 cut(s) 5, 242
HhaI GCGC 1 cut(s) 933
Hin1II CATG 5 cut(s) 112, 152, 928, 964, 1048
Hin6I GCGC 1 cut(s) 931
HinP1I GCGC 1 cut(s) 931
HindIII AAGCTT 2 cut(s) 665, 981
HinfI GANTC 7 cut(s) 41, 188, 312, 917, 999, 1055, 1394
HpaII CCGG 2 cut(s) 5, 242
HphI GGTGA 5 cut(s) 176, 244, 362, 443, 449
Hpy166II GTNNAC 3 cut(s) 13, 644, 1354
Hpy188I TCNGA 6 cut(s) 46, 489, 627, 814, 1022, 1264
Hpy8I GTNNAC 3 cut(s) 13, 644, 1354
Hpy99I CGWCG 1 cut(s) 545
HpyAV CCTTC 4 cut(s) 43, 176, 428, 562
HpyCH4III ACNGT 5 cut(s) 17, 472, 577, 778, 820
HpyCH4IV ACGT 8 cut(s) 69, 250, 367, 540, 640, 844, 1150, 1447
HpyCH4V TGCA 6 cut(s) 338, 506, 659, 1118, 1280, 1459
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 119, 980, 1142, 1154
HpyF3I CTNAG 4 cut(s) 811, 1074, 1261, 1286
HpySE526I ACGT 8 cut(s) 69, 250, 367, 540, 640, 844, 1150, 1447
Hsp92II CATG 5 cut(s) 112, 152, 928, 964, 1048
HspAI GCGC 1 cut(s) 931
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 439, 1405
Kzo9I GATC 8 cut(s) 234, 238, 342, 439, 1405, 1414, 1468, 1472
LmnI GCTCC 2 cut(s) 116, 493
Lsp1109I GCAGC 4 cut(s) 106, 658, 709, 1157
LweI GCATC 5 cut(s) 508, 668, 700, 1166, 1496
MabI ACCWGGT 1 cut(s) 550
MaeI CTAG 1 cut(s) 648
MaeII ACGT 8 cut(s) 69, 250, 367, 540, 640, 844, 1150, 1447
MaeIII GTNAC 3 cut(s) 532, 1219, 1270
MalI GATC 8 cut(s) 236, 240, 344, 441, 1407, 1416, 1470, 1474
MbiI CCGCTC 1 cut(s) 974
MboI GATC 8 cut(s) 234, 238, 342, 439, 1405, 1414, 1468, 1472
MboII GAAGA 3 cut(s) 466, 926, 1478
MfeI CAATTG 2 cut(s) 884, 1224
MflI RGATCY 1 cut(s) 342
MhlI GDGCHC 2 cut(s) 22, 498
MlsI TGGCCA 1 cut(s) 947
MluCI AATT 9 cut(s) 154, 168, 198, 426, 611, 884, 1224, 1409, 1483
MluNI TGGCCA 1 cut(s) 947
MlyI GAGTC 2 cut(s) 35, 1403
MmeI TCCRAC 2 cut(s) 124, 1514
Mox20I TGGCCA 1 cut(s) 947
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 2 cut(s) 171, 1340
MscI TGGCCA 1 cut(s) 947
MslI CAYNNNNRTG 2 cut(s) 965, 1066
Msp20I TGGCCA 1 cut(s) 947
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 2 cut(s) 5, 242
MspR9I CCNGG 4 cut(s) 285, 552, 753, 1248
MunI CAATTG 2 cut(s) 884, 1224
MvaI CCWGG 4 cut(s) 285, 552, 753, 1248
MwoI GCNNNNNNNGC 5 cut(s) 72, 119, 980, 1142, 1154
NaeI GCCGGC 1 cut(s) 6
NdeII GATC 8 cut(s) 234, 238, 342, 439, 1405, 1414, 1468, 1472
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 5 cut(s) 112, 152, 928, 964, 1048
NlaIV GGNNCC 2 cut(s) 19, 282
NmuCI GTSAC 2 cut(s) 1219, 1270
NspI RCATGY 1 cut(s) 964
NspV TTCGAA 1 cut(s) 477
PagI TCATGA 1 cut(s) 924
PceI AGGCCT 1 cut(s) 652
PcsI WCGNNNNNNNCGW 2 cut(s) 1468, 1477
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 2 cut(s) 171, 1340
PfeI GAWTC 5 cut(s) 188, 312, 917, 999, 1055
PfoI TCCNGGA 1 cut(s) 1246
PkrI GCNGC 6 cut(s) 121, 673, 699, 973, 1101, 1147
PleI GAGTC 2 cut(s) 35, 1402
PmaCI CACGTG 2 cut(s) 368, 1448
PmlI CACGTG 2 cut(s) 368, 1448
PpsI GAGTC 2 cut(s) 35, 1402
Ppu21I YACGTR 4 cut(s) 251, 368, 641, 1448
PpuMI RGGWCCY 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 281
Psp6I CCWGG 4 cut(s) 283, 550, 751, 1246
PspCI CACGTG 2 cut(s) 368, 1448
PspFI CCCAGC 1 cut(s) 221
PspGI CCWGG 4 cut(s) 283, 550, 751, 1246
PspN4I GGNNCC 2 cut(s) 19, 282
PspPI GGNCC 4 cut(s) 86, 281, 321, 1199
PspPPI RGGWCCY 1 cut(s) 281
PstI CTGCAG 1 cut(s) 1120
PstNI CAGNNNCTG 2 cut(s) 818, 1268
PsuI RGATCY 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 7 cut(s) 128, 253, 464, 639, 643, 803, 1379
RsaNI GTAC 7 cut(s) 127, 252, 463, 638, 642, 802, 1378
RseI CAYNNNNRTG 2 cut(s) 965, 1066
SatI GCNGC 6 cut(s) 120, 672, 698, 972, 1100, 1146
Sau3AI GATC 8 cut(s) 234, 238, 342, 439, 1405, 1414, 1468, 1472
Sau96I GGNCC 4 cut(s) 86, 281, 321, 1199
ScaI AGTACT 2 cut(s) 803, 1379
SchI GAGTC 2 cut(s) 35, 1403
ScrFI CCNGG 4 cut(s) 285, 552, 753, 1248
SduI GDGCHC 2 cut(s) 22, 498
SexAI ACCWGGT 1 cut(s) 550
SfaNI GCATC 5 cut(s) 508, 668, 700, 1166, 1496
SfcI CTRYAG 1 cut(s) 1116
SfuI TTCGAA 1 cut(s) 477
SinI GGWCC 2 cut(s) 86, 281
SmiMI CAYNNNNRTG 2 cut(s) 965, 1066
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
SnaBI TACGTA 2 cut(s) 251, 641
Sse9I AATT 9 cut(s) 154, 168, 198, 426, 611, 884, 1224, 1409, 1483
SseBI AGGCCT 1 cut(s) 652
SsiI CCGC 4 cut(s) 8, 73, 972, 1099
SspI AATATT 3 cut(s) 291, 301, 560
SspMI CTAG 1 cut(s) 648
StuI AGGCCT 1 cut(s) 652
StyD4I CCNGG 4 cut(s) 283, 550, 751, 1246
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 5 cut(s) 17, 472, 577, 778, 820
TaiI ACGT 8 cut(s) 72, 253, 370, 543, 643, 847, 1153, 1450
TaqI TCGA 6 cut(s) 237, 477, 1066, 1373, 1413, 1471
TasI AATT 9 cut(s) 154, 168, 198, 426, 611, 884, 1224, 1409, 1483
TatI WGTACW 2 cut(s) 801, 1377
TauI GCSGC 2 cut(s) 974, 1102
TfiI GAWTC 5 cut(s) 188, 312, 917, 999, 1055
TscAI CASTG 4 cut(s) 276, 697, 823, 1222
TseFI GTSAC 2 cut(s) 1219, 1270
TseI GCWGC 4 cut(s) 119, 671, 697, 1145
Tsp45I GTSAC 2 cut(s) 1219, 1270
TspDTI ATGAA 3 cut(s) 552, 867, 941
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 4 cut(s) 276, 697, 823, 1222
VpaK11BI GGWCC 2 cut(s) 86, 281
XagI CCTNNNNNAGG 1 cut(s) 1248
XapI RAATTY 1 cut(s) 611
XceI RCATGY 1 cut(s) 964
XmaJI CCTAGG 1 cut(s) 647
XmiI GTMKAC 1 cut(s) 12
XmnI GAANNNNTTC 2 cut(s) 171, 1340
XspI CTAG 1 cut(s) 648
ZrmI AGTACT 2 cut(s) 803, 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.